TY  - JOUR
AU  - Trivanović, Drenka
AU  - Jauković, Aleksandra
AU  - Popović, Branka
AU  - Krstić, Jelena
AU  - Mojsilović, Slavko
AU  - Okic-Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Santibanez, Juan Francisco
AU  - Bugarski, Diana
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2018
AB  - Aims: In vitro expansion changes replication and differentiation capacity of mesenchymal stem cells (MSCs), increasing challenges and risks, while limiting the sufficient number of MSCs required for cytotherapy. Here, we characterized and compared proliferation, differentiation, telomere length and pluripotency marker expression in MSCs of various origins. Main methods: Immunophenotyping, proliferation and differentiation assays were performed. Pluripotency marker (Nanog, Oct-4, SOX-2, SSEA-4) expression was determined by immunofluorescence. Quantitative PCR was performed for relative telomere length (RTL) analyses, while expression of relevant genes for pluripotency markers, differentiation state (Cbfa1, human placental alkaline phosphatase, peroxisome proliferator activated receptor, Sox9 and Collagen II a1), and telomerase reverse transcriptase (hTERT) was determined by semiquantitative RT-PCR. Key findings: Peripheral blood MSCs (PB-MSCs) and umbilical cord MSCs (UC-MSCs) showed the highest, while periodontal ligament MSCs (PDL-MSCs) and adipose tissue MSCs (AT-MSCs) the lowest values of both the replication potential and RTL. Although MSCs from exfoliated deciduous teeth (SHEDs), PDL-MSCs and AT-MSCs showed higher mRNA expression of pluripotency markers, all MSCs expressed pluripotency marker proteins. SHEDs and PDL-MSCs showed prominent capacity for osteogenesis, PB-MSCs and UC-MSCs showed strengthened adipogenic differentiation potential, while AT-MSCs displayed similar differentiation into both lines. Significance: The MSCs populations derived from different sources, although displaying similar phenotype, exhibited high degree of variability regarding biological properties related to their self-renewal and differentiation capacity. These data indicate that for more accurate use in cell therapy, individualities of MSCs isolated from different tissues should be identified and taken into consideration when planning their use in clinical protocols.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression
VL  - 141
SP  - 61
EP  - 73
DO  - 10.1016/j.lfs.2015.09.019
ER  - 

@article{
author = "Trivanović, Drenka and Jauković, Aleksandra and Popović, Branka and Krstić, Jelena and Mojsilović, Slavko and Okic-Đorđević, Ivana and Kukolj, Tamara and Obradović, Hristina and Santibanez, Juan Francisco and Bugarski, Diana",
year = "2015",
abstract = "Aims: In vitro expansion changes replication and differentiation capacity of mesenchymal stem cells (MSCs), increasing challenges and risks, while limiting the sufficient number of MSCs required for cytotherapy. Here, we characterized and compared proliferation, differentiation, telomere length and pluripotency marker expression in MSCs of various origins. Main methods: Immunophenotyping, proliferation and differentiation assays were performed. Pluripotency marker (Nanog, Oct-4, SOX-2, SSEA-4) expression was determined by immunofluorescence. Quantitative PCR was performed for relative telomere length (RTL) analyses, while expression of relevant genes for pluripotency markers, differentiation state (Cbfa1, human placental alkaline phosphatase, peroxisome proliferator activated receptor, Sox9 and Collagen II a1), and telomerase reverse transcriptase (hTERT) was determined by semiquantitative RT-PCR. Key findings: Peripheral blood MSCs (PB-MSCs) and umbilical cord MSCs (UC-MSCs) showed the highest, while periodontal ligament MSCs (PDL-MSCs) and adipose tissue MSCs (AT-MSCs) the lowest values of both the replication potential and RTL. Although MSCs from exfoliated deciduous teeth (SHEDs), PDL-MSCs and AT-MSCs showed higher mRNA expression of pluripotency markers, all MSCs expressed pluripotency marker proteins. SHEDs and PDL-MSCs showed prominent capacity for osteogenesis, PB-MSCs and UC-MSCs showed strengthened adipogenic differentiation potential, while AT-MSCs displayed similar differentiation into both lines. Significance: The MSCs populations derived from different sources, although displaying similar phenotype, exhibited high degree of variability regarding biological properties related to their self-renewal and differentiation capacity. These data indicate that for more accurate use in cell therapy, individualities of MSCs isolated from different tissues should be identified and taken into consideration when planning their use in clinical protocols.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression",
volume = "141",
pages = "61-73",
doi = "10.1016/j.lfs.2015.09.019"
}

Trivanović, D., Jauković, A., Popović, B., Krstić, J., Mojsilović, S., Okic-Đorđević, I., Kukolj, T., Obradović, H., Santibanez, J. F.,& Bugarski, D.. (2015). Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 141, 61-73.
https://doi.org/10.1016/j.lfs.2015.09.019

Trivanović D, Jauković A, Popović B, Krstić J, Mojsilović S, Okic-Đorđević I, Kukolj T, Obradović H, Santibanez JF, Bugarski D. Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression. in Life Sciences. 2015;141:61-73.
doi:10.1016/j.lfs.2015.09.019 .

Trivanović, Drenka, Jauković, Aleksandra, Popović, Branka, Krstić, Jelena, Mojsilović, Slavko, Okic-Đorđević, Ivana, Kukolj, Tamara, Obradović, Hristina, Santibanez, Juan Francisco, Bugarski, Diana, "Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression" in Life Sciences, 141 (2015):61-73,
https://doi.org/10.1016/j.lfs.2015.09.019 . .

TY  - JOUR
AU  - Miletić, Maja
AU  - Mojsilović, S.
AU  - Okic-Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Jauković, Aleksandra
AU  - Santibanez, J. F.
AU  - Jovcić, Gordana
AU  - Bugarski, Diana
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1951
AB  - Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Mesenchymal stem cells isolated from human periodontal ligament
VL  - 66
IS  - 1
SP  - 261
EP  - 271
DO  - 10.2298/ABS1401261M
ER  - 

@article{
author = "Miletić, Maja and Mojsilović, S. and Okic-Đorđević, Ivana and Kukolj, Tamara and Jauković, Aleksandra and Santibanez, J. F. and Jovcić, Gordana and Bugarski, Diana",
year = "2014",
abstract = "Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Mesenchymal stem cells isolated from human periodontal ligament",
volume = "66",
number = "1",
pages = "261-271",
doi = "10.2298/ABS1401261M"
}

Miletić, M., Mojsilović, S., Okic-Đorđević, I., Kukolj, T., Jauković, A., Santibanez, J. F., Jovcić, G.,& Bugarski, D.. (2014). Mesenchymal stem cells isolated from human periodontal ligament. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(1), 261-271.
https://doi.org/10.2298/ABS1401261M

Miletić M, Mojsilović S, Okic-Đorđević I, Kukolj T, Jauković A, Santibanez JF, Jovcić G, Bugarski D. Mesenchymal stem cells isolated from human periodontal ligament. in Archives of Biological Sciences. 2014;66(1):261-271.
doi:10.2298/ABS1401261M .

Miletić, Maja, Mojsilović, S., Okic-Đorđević, Ivana, Kukolj, Tamara, Jauković, Aleksandra, Santibanez, J. F., Jovcić, Gordana, Bugarski, Diana, "Mesenchymal stem cells isolated from human periodontal ligament" in Archives of Biological Sciences, 66, no. 1 (2014):261-271,
https://doi.org/10.2298/ABS1401261M . .

TY  - JOUR
AU  - Miletić, Maja
AU  - Mojsilović, S.
AU  - Okic-Đorđević, Ivana
AU  - Maletić, D.
AU  - Puač, Nevena
AU  - Lazović, Saša
AU  - Malović, Gordana
AU  - Milenković, P.
AU  - Petrović, Zoran Lj.
AU  - Bugarski, Diana
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1833
AB  - Here we investigate the influences of non-thermal atmospheric plasma on human mesenchymal stem cells isolated from periodontal ligament (hPDL-MSCs). A specially redesigned plasma needle was used as the source of low-temperature plasma and its effects on different hPDL-MSC functions were investigated. Cell cultures were obtained from extracted normal impacted third molars and characterized for their phenotype and multi-potential differentiation. The hPDL-MSCs possessed all the typical MSC properties, including clonogenic ability, high proliferation rate, specific phenotype and multilineage differentiation. The data regarding the interaction of plasma with hPDL-MSCs demonstrated that plasma treatment inhibited the migration of hPDL-MSCs and induced some detachment, while not affecting their viability. Additionally, plasma significantly attenuated hPDL-MSCs' proliferation, but promoted their osteogenic differentiation. The results of this study indicated that a non-thermal plasma offers specific activity with non-destructive properties that can be advantageous for future dental applications.
PB  - Iop Publishing Ltd, Bristol
T2  - Journal of Physics D - Applied Physics
T1  - Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells
VL  - 46
IS  - 34
DO  - 10.1088/0022-3727/46/34/345401
ER  - 

@article{
author = "Miletić, Maja and Mojsilović, S. and Okic-Đorđević, Ivana and Maletić, D. and Puač, Nevena and Lazović, Saša and Malović, Gordana and Milenković, P. and Petrović, Zoran Lj. and Bugarski, Diana",
year = "2013",
abstract = "Here we investigate the influences of non-thermal atmospheric plasma on human mesenchymal stem cells isolated from periodontal ligament (hPDL-MSCs). A specially redesigned plasma needle was used as the source of low-temperature plasma and its effects on different hPDL-MSC functions were investigated. Cell cultures were obtained from extracted normal impacted third molars and characterized for their phenotype and multi-potential differentiation. The hPDL-MSCs possessed all the typical MSC properties, including clonogenic ability, high proliferation rate, specific phenotype and multilineage differentiation. The data regarding the interaction of plasma with hPDL-MSCs demonstrated that plasma treatment inhibited the migration of hPDL-MSCs and induced some detachment, while not affecting their viability. Additionally, plasma significantly attenuated hPDL-MSCs' proliferation, but promoted their osteogenic differentiation. The results of this study indicated that a non-thermal plasma offers specific activity with non-destructive properties that can be advantageous for future dental applications.",
publisher = "Iop Publishing Ltd, Bristol",
journal = "Journal of Physics D - Applied Physics",
title = "Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells",
volume = "46",
number = "34",
doi = "10.1088/0022-3727/46/34/345401"
}

Miletić, M., Mojsilović, S., Okic-Đorđević, I., Maletić, D., Puač, N., Lazović, S., Malović, G., Milenković, P., Petrović, Z. Lj.,& Bugarski, D.. (2013). Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells. in Journal of Physics D - Applied Physics
Iop Publishing Ltd, Bristol., 46(34).
https://doi.org/10.1088/0022-3727/46/34/345401

Miletić M, Mojsilović S, Okic-Đorđević I, Maletić D, Puač N, Lazović S, Malović G, Milenković P, Petrović ZL, Bugarski D. Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells. in Journal of Physics D - Applied Physics. 2013;46(34).
doi:10.1088/0022-3727/46/34/345401 .

Miletić, Maja, Mojsilović, S., Okic-Đorđević, Ivana, Maletić, D., Puač, Nevena, Lazović, Saša, Malović, Gordana, Milenković, P., Petrović, Zoran Lj., Bugarski, Diana, "Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells" in Journal of Physics D - Applied Physics, 46, no. 34 (2013),
https://doi.org/10.1088/0022-3727/46/34/345401 . .