TY  - JOUR
AU  - Kanjevac, Tatjana
AU  - Milovanović, Marija
AU  - Volarević, Vladislav
AU  - Lukić, Miodrag L.
AU  - Arsenijević, Nebojša
AU  - Marković, Dejan
AU  - Zdravković, Nebojša
AU  - Tešić, Živoslav
AU  - Lukić, Aleksandra
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1752
AB  - Objectives: Glass ionomer cements (GICs) are commonly used as restorative materials. Responses to GICs differ among cell types and it is therefore of importance to thoroughly investigate the influence of these restorative materials on pulp stem cells that are potential source for dental tissue regeneration. Eight biomaterials were tested: Fuji I, Fuji II, Fuji VIII, Fuji IX, Fuji Plus, Fuji Triage, Vitrebond and Composit. We compared their cytotoxic activity on human dental pulp stem cells (DPSC) and correlated this activity with the content of Fluoride, Aluminium and Strontium ions in their eluates. Methods: Elution samples of biomaterials were prepared in sterile tissue culture medium and the medium was tested for toxicity by an assay of cell survival/proliferation (MTT test) and apoptosis (Annexin V FITC Detection Kit). Concentrations of Fluoride, Aluminium and Strontium ions were tested by appropriate methods in the same eluates. Results: Cell survival ranged between 79.62% (Fuji Triage) to 1.5% (Fuji Plus) and most dead DPSCs were in the stage of late apoptosis. Fluoride release correlated with cytotoxicity of GICs, while Aluminium and Strontium ions, present in significant amount in eluates of tested GICs did not. Significance: Fuji Plus, Vitrebond and Fuji VIII, which released fluoride in higher quantities than other GICs, were highly toxic to human DPSCs. Opposite, low levels of released fluoride correlated to low cytotoxic effect of Composit, Fuji I and Fuji Triage.
PB  - Bentham Science Publ Ltd, Sharjah
T2  - Medicinal Chemistry
T1  - Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release
VL  - 8
IS  - 1
SP  - 40
EP  - 45
DO  - 10.2174/157340612799278351
ER  - 

@article{
author = "Kanjevac, Tatjana and Milovanović, Marija and Volarević, Vladislav and Lukić, Miodrag L. and Arsenijević, Nebojša and Marković, Dejan and Zdravković, Nebojša and Tešić, Živoslav and Lukić, Aleksandra",
year = "2012",
abstract = "Objectives: Glass ionomer cements (GICs) are commonly used as restorative materials. Responses to GICs differ among cell types and it is therefore of importance to thoroughly investigate the influence of these restorative materials on pulp stem cells that are potential source for dental tissue regeneration. Eight biomaterials were tested: Fuji I, Fuji II, Fuji VIII, Fuji IX, Fuji Plus, Fuji Triage, Vitrebond and Composit. We compared their cytotoxic activity on human dental pulp stem cells (DPSC) and correlated this activity with the content of Fluoride, Aluminium and Strontium ions in their eluates. Methods: Elution samples of biomaterials were prepared in sterile tissue culture medium and the medium was tested for toxicity by an assay of cell survival/proliferation (MTT test) and apoptosis (Annexin V FITC Detection Kit). Concentrations of Fluoride, Aluminium and Strontium ions were tested by appropriate methods in the same eluates. Results: Cell survival ranged between 79.62% (Fuji Triage) to 1.5% (Fuji Plus) and most dead DPSCs were in the stage of late apoptosis. Fluoride release correlated with cytotoxicity of GICs, while Aluminium and Strontium ions, present in significant amount in eluates of tested GICs did not. Significance: Fuji Plus, Vitrebond and Fuji VIII, which released fluoride in higher quantities than other GICs, were highly toxic to human DPSCs. Opposite, low levels of released fluoride correlated to low cytotoxic effect of Composit, Fuji I and Fuji Triage.",
publisher = "Bentham Science Publ Ltd, Sharjah",
journal = "Medicinal Chemistry",
title = "Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release",
volume = "8",
number = "1",
pages = "40-45",
doi = "10.2174/157340612799278351"
}

Kanjevac, T., Milovanović, M., Volarević, V., Lukić, M. L., Arsenijević, N., Marković, D., Zdravković, N., Tešić, Ž.,& Lukić, A.. (2012). Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release. in Medicinal Chemistry
Bentham Science Publ Ltd, Sharjah., 8(1), 40-45.
https://doi.org/10.2174/157340612799278351

Kanjevac T, Milovanović M, Volarević V, Lukić ML, Arsenijević N, Marković D, Zdravković N, Tešić Ž, Lukić A. Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release. in Medicinal Chemistry. 2012;8(1):40-45.
doi:10.2174/157340612799278351 .

Kanjevac, Tatjana, Milovanović, Marija, Volarević, Vladislav, Lukić, Miodrag L., Arsenijević, Nebojša, Marković, Dejan, Zdravković, Nebojša, Tešić, Živoslav, Lukić, Aleksandra, "Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release" in Medicinal Chemistry, 8, no. 1 (2012):40-45,
https://doi.org/10.2174/157340612799278351 . .

TY  - JOUR
AU  - Marković, Dejan
AU  - Milenković, Ana
AU  - Koliakos, George
AU  - Kostidou, Elena
AU  - Karadžić, Ivana
AU  - Debeljak-Martačić, Jasmina
AU  - Jokanović, Vukoman
AU  - Perić, Tamara
AU  - Petrović, Bojan
AU  - Arsenijević, Nebojša
AU  - Kanjevac, Tatjana
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1517
AB  - Dental pulp stem cells (DPSCs) as postnatal stem cells have recently been described. They are clonogenic cells, capable for self-renewal with high proliferative potential. Their multilineage potential and plasticity enables their differentiation into different kind of cells, such as osteoblasts, chondrocytes, adipocytes, muscle cells, neural cells, odontoblasts, cementoblasts and ameloblasts. DPSCs are an important human stem cells source, especially in patients who lost their chance for umbilical cord blood isolation and preservation. As these cells became useful for tissue engineering and cell therapy, proper mode of their preservation also became important. The most important points in the cryopreservation and recovery procedure are: growth phase of harvested cells, number of cells, the proper cryopreservative concentration and serum concentration. The cryopreservation process includes the following general components: harvesting of the cells, addition of cryopreservative, the freezing procedure, the thawing procedure and assessment of the viability prior to transplantation. There is no single and perfect cryopreservation method. Further investigations should be regarding capability of DPSCs and their differentiated cells to recover and restart proliferation, differentiation and new tissue production for therapeutic use after cryopreservation.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Stomatology
T1  - Potential preservation of dental pulp stem cells
VL  - 14
IS  - 1
SP  - 4
EP  - 7
UR  - https://hdl.handle.net/21.15107/rcub_smile_1517
ER  - 

@article{
author = "Marković, Dejan and Milenković, Ana and Koliakos, George and Kostidou, Elena and Karadžić, Ivana and Debeljak-Martačić, Jasmina and Jokanović, Vukoman and Perić, Tamara and Petrović, Bojan and Arsenijević, Nebojša and Kanjevac, Tatjana",
year = "2010",
abstract = "Dental pulp stem cells (DPSCs) as postnatal stem cells have recently been described. They are clonogenic cells, capable for self-renewal with high proliferative potential. Their multilineage potential and plasticity enables their differentiation into different kind of cells, such as osteoblasts, chondrocytes, adipocytes, muscle cells, neural cells, odontoblasts, cementoblasts and ameloblasts. DPSCs are an important human stem cells source, especially in patients who lost their chance for umbilical cord blood isolation and preservation. As these cells became useful for tissue engineering and cell therapy, proper mode of their preservation also became important. The most important points in the cryopreservation and recovery procedure are: growth phase of harvested cells, number of cells, the proper cryopreservative concentration and serum concentration. The cryopreservation process includes the following general components: harvesting of the cells, addition of cryopreservative, the freezing procedure, the thawing procedure and assessment of the viability prior to transplantation. There is no single and perfect cryopreservation method. Further investigations should be regarding capability of DPSCs and their differentiated cells to recover and restart proliferation, differentiation and new tissue production for therapeutic use after cryopreservation.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Stomatology",
title = "Potential preservation of dental pulp stem cells",
volume = "14",
number = "1",
pages = "4-7",
url = "https://hdl.handle.net/21.15107/rcub_smile_1517"
}

Marković, D., Milenković, A., Koliakos, G., Kostidou, E., Karadžić, I., Debeljak-Martačić, J., Jokanović, V., Perić, T., Petrović, B., Arsenijević, N.,& Kanjevac, T.. (2010). Potential preservation of dental pulp stem cells. in Balkan Journal of Stomatology
Udruženje stomatologa Balkana., 14(1), 4-7.
https://hdl.handle.net/21.15107/rcub_smile_1517

Marković D, Milenković A, Koliakos G, Kostidou E, Karadžić I, Debeljak-Martačić J, Jokanović V, Perić T, Petrović B, Arsenijević N, Kanjevac T. Potential preservation of dental pulp stem cells. in Balkan Journal of Stomatology. 2010;14(1):4-7.
https://hdl.handle.net/21.15107/rcub_smile_1517 .

Marković, Dejan, Milenković, Ana, Koliakos, George, Kostidou, Elena, Karadžić, Ivana, Debeljak-Martačić, Jasmina, Jokanović, Vukoman, Perić, Tamara, Petrović, Bojan, Arsenijević, Nebojša, Kanjevac, Tatjana, "Potential preservation of dental pulp stem cells" in Balkan Journal of Stomatology, 14, no. 1 (2010):4-7,
https://hdl.handle.net/21.15107/rcub_smile_1517 .