TY  - JOUR
AU  - Karadžić, Ivana
AU  - Vučić, Vesna
AU  - Jokanović, Vukoman
AU  - Debeljak-Martačić, Jasmina
AU  - Marković, Dejan
AU  - Petrović, Snježana
AU  - Glibetić, Marija
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2063
AB  - The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells
VL  - 103
IS  - 1
SP  - 350
EP  - 357
DO  - 10.1002/jbm.a.35180
ER  - 

@article{
author = "Karadžić, Ivana and Vučić, Vesna and Jokanović, Vukoman and Debeljak-Martačić, Jasmina and Marković, Dejan and Petrović, Snježana and Glibetić, Marija",
year = "2015",
abstract = "The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells",
volume = "103",
number = "1",
pages = "350-357",
doi = "10.1002/jbm.a.35180"
}

Karadžić, I., Vučić, V., Jokanović, V., Debeljak-Martačić, J., Marković, D., Petrović, S.,& Glibetić, M.. (2015). Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research Part A
Wiley-Blackwell, Hoboken., 103(1), 350-357.
https://doi.org/10.1002/jbm.a.35180

Karadžić I, Vučić V, Jokanović V, Debeljak-Martačić J, Marković D, Petrović S, Glibetić M. Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research Part A. 2015;103(1):350-357.
doi:10.1002/jbm.a.35180 .

Karadžić, Ivana, Vučić, Vesna, Jokanović, Vukoman, Debeljak-Martačić, Jasmina, Marković, Dejan, Petrović, Snježana, Glibetić, Marija, "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells" in Journal of Biomedical Materials Research Part A, 103, no. 1 (2015):350-357,
https://doi.org/10.1002/jbm.a.35180 . .

TY  - JOUR
AU  - Marković, Dejan
AU  - Milenković, Ana
AU  - Koliakos, George
AU  - Kostidou, Elena
AU  - Karadžić, Ivana
AU  - Debeljak-Martačić, Jasmina
AU  - Jokanović, Vukoman
AU  - Perić, Tamara
AU  - Petrović, Bojan
AU  - Arsenijević, Nebojša
AU  - Kanjevac, Tatjana
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1517
AB  - Dental pulp stem cells (DPSCs) as postnatal stem cells have recently been described. They are clonogenic cells, capable for self-renewal with high proliferative potential. Their multilineage potential and plasticity enables their differentiation into different kind of cells, such as osteoblasts, chondrocytes, adipocytes, muscle cells, neural cells, odontoblasts, cementoblasts and ameloblasts. DPSCs are an important human stem cells source, especially in patients who lost their chance for umbilical cord blood isolation and preservation. As these cells became useful for tissue engineering and cell therapy, proper mode of their preservation also became important. The most important points in the cryopreservation and recovery procedure are: growth phase of harvested cells, number of cells, the proper cryopreservative concentration and serum concentration. The cryopreservation process includes the following general components: harvesting of the cells, addition of cryopreservative, the freezing procedure, the thawing procedure and assessment of the viability prior to transplantation. There is no single and perfect cryopreservation method. Further investigations should be regarding capability of DPSCs and their differentiated cells to recover and restart proliferation, differentiation and new tissue production for therapeutic use after cryopreservation.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Stomatology
T1  - Potential preservation of dental pulp stem cells
VL  - 14
IS  - 1
SP  - 4
EP  - 7
UR  - https://hdl.handle.net/21.15107/rcub_smile_1517
ER  - 

@article{
author = "Marković, Dejan and Milenković, Ana and Koliakos, George and Kostidou, Elena and Karadžić, Ivana and Debeljak-Martačić, Jasmina and Jokanović, Vukoman and Perić, Tamara and Petrović, Bojan and Arsenijević, Nebojša and Kanjevac, Tatjana",
year = "2010",
abstract = "Dental pulp stem cells (DPSCs) as postnatal stem cells have recently been described. They are clonogenic cells, capable for self-renewal with high proliferative potential. Their multilineage potential and plasticity enables their differentiation into different kind of cells, such as osteoblasts, chondrocytes, adipocytes, muscle cells, neural cells, odontoblasts, cementoblasts and ameloblasts. DPSCs are an important human stem cells source, especially in patients who lost their chance for umbilical cord blood isolation and preservation. As these cells became useful for tissue engineering and cell therapy, proper mode of their preservation also became important. The most important points in the cryopreservation and recovery procedure are: growth phase of harvested cells, number of cells, the proper cryopreservative concentration and serum concentration. The cryopreservation process includes the following general components: harvesting of the cells, addition of cryopreservative, the freezing procedure, the thawing procedure and assessment of the viability prior to transplantation. There is no single and perfect cryopreservation method. Further investigations should be regarding capability of DPSCs and their differentiated cells to recover and restart proliferation, differentiation and new tissue production for therapeutic use after cryopreservation.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Stomatology",
title = "Potential preservation of dental pulp stem cells",
volume = "14",
number = "1",
pages = "4-7",
url = "https://hdl.handle.net/21.15107/rcub_smile_1517"
}

Marković, D., Milenković, A., Koliakos, G., Kostidou, E., Karadžić, I., Debeljak-Martačić, J., Jokanović, V., Perić, T., Petrović, B., Arsenijević, N.,& Kanjevac, T.. (2010). Potential preservation of dental pulp stem cells. in Balkan Journal of Stomatology
Udruženje stomatologa Balkana., 14(1), 4-7.
https://hdl.handle.net/21.15107/rcub_smile_1517

Marković D, Milenković A, Koliakos G, Kostidou E, Karadžić I, Debeljak-Martačić J, Jokanović V, Perić T, Petrović B, Arsenijević N, Kanjevac T. Potential preservation of dental pulp stem cells. in Balkan Journal of Stomatology. 2010;14(1):4-7.
https://hdl.handle.net/21.15107/rcub_smile_1517 .

Marković, Dejan, Milenković, Ana, Koliakos, George, Kostidou, Elena, Karadžić, Ivana, Debeljak-Martačić, Jasmina, Jokanović, Vukoman, Perić, Tamara, Petrović, Bojan, Arsenijević, Nebojša, Kanjevac, Tatjana, "Potential preservation of dental pulp stem cells" in Balkan Journal of Stomatology, 14, no. 1 (2010):4-7,
https://hdl.handle.net/21.15107/rcub_smile_1517 .