TY  - JOUR
AU  - Petrović, V.
AU  - Opačić-Galić, Vanja
AU  - Živković, Slavoljub
AU  - Nikolić, B.
AU  - Danilović, Vesna
AU  - Miletić, Vesna
AU  - Jokanović, Vukoman
AU  - Mitić-Ćulafić, Dragana
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2518
AB  - AimTo evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems (CS) and hydroxyapatite (HA-CS). MethodologyCytotoxicity of eluates of new nanostructural noncommercial materials CS and HA-CS, and MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil) as a control, were tested using the MTT assay on MRC-5 cells. Eluates of set materials were tested in 100% and 50% concentrations, 24h, 7days and 21days post-elution. The pH values were determined for undiluted eluates of set materials. Polyethylene tubes containing the test materials (CS, HA-CS, MTA) were implanted in subcutaneous tissue of Wistar rats. Histopathological examinations were conducted at 7, 15, 30 and 60days after the implantation. Data were statistically analyzed using three-way and one-way anova Tukey's post hoc test as well as Kruskall-Wallis test with Dunn's post hoc test at =0.05. ResultsAll materials significantly reduced cell viability; especially when undiluted eluates were used (P lt 0.001). After 24h elution, cell viability was 101.8%, 49.5 +/- 4.2% and 61 +/- 7.4%, for MTA, and HA-CS, respectively. However, CS and HA-CS were significantly less toxic than the control material MTA (P lt 0.05). Cytotoxicity could be at least partially attributed to pH kinetics over time. Dilution of eluates of all tested materials resulted in better cell survival. Histopathological examination indicated similar inflammatory reaction, vascular congestion and connective tissue integrity associated with CS, HA-CS and MTA at each observation period (P>0.05). The only significant difference was found for capsule thickness, that is thicker capsule was associated with HA-CS compared to MTA at 60days (P=0.0039). HA-CS induced moderately thick capsules (median score 3, score range 2-3), whereas MTA resulted in thin capsule formation (median score 2, score range 1-3). ConclusionsEvaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil).
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study
VL  - 48
IS  - 10
SP  - 966
EP  - 975
DO  - 10.1111/iej.12391
ER  - 

@article{
author = "Petrović, V. and Opačić-Galić, Vanja and Živković, Slavoljub and Nikolić, B. and Danilović, Vesna and Miletić, Vesna and Jokanović, Vukoman and Mitić-Ćulafić, Dragana",
year = "2015",
abstract = "AimTo evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems (CS) and hydroxyapatite (HA-CS). MethodologyCytotoxicity of eluates of new nanostructural noncommercial materials CS and HA-CS, and MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil) as a control, were tested using the MTT assay on MRC-5 cells. Eluates of set materials were tested in 100% and 50% concentrations, 24h, 7days and 21days post-elution. The pH values were determined for undiluted eluates of set materials. Polyethylene tubes containing the test materials (CS, HA-CS, MTA) were implanted in subcutaneous tissue of Wistar rats. Histopathological examinations were conducted at 7, 15, 30 and 60days after the implantation. Data were statistically analyzed using three-way and one-way anova Tukey's post hoc test as well as Kruskall-Wallis test with Dunn's post hoc test at =0.05. ResultsAll materials significantly reduced cell viability; especially when undiluted eluates were used (P lt 0.001). After 24h elution, cell viability was 101.8%, 49.5 +/- 4.2% and 61 +/- 7.4%, for MTA, and HA-CS, respectively. However, CS and HA-CS were significantly less toxic than the control material MTA (P lt 0.05). Cytotoxicity could be at least partially attributed to pH kinetics over time. Dilution of eluates of all tested materials resulted in better cell survival. Histopathological examination indicated similar inflammatory reaction, vascular congestion and connective tissue integrity associated with CS, HA-CS and MTA at each observation period (P>0.05). The only significant difference was found for capsule thickness, that is thicker capsule was associated with HA-CS compared to MTA at 60days (P=0.0039). HA-CS induced moderately thick capsules (median score 3, score range 2-3), whereas MTA resulted in thin capsule formation (median score 2, score range 1-3). ConclusionsEvaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil).",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study",
volume = "48",
number = "10",
pages = "966-975",
doi = "10.1111/iej.12391"
}

Petrović, V., Opačić-Galić, V., Živković, S., Nikolić, B., Danilović, V., Miletić, V., Jokanović, V.,& Mitić-Ćulafić, D.. (2015). Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study. in International Endodontic Journal
Wiley, Hoboken., 48(10), 966-975.
https://doi.org/10.1111/iej.12391

Petrović V, Opačić-Galić V, Živković S, Nikolić B, Danilović V, Miletić V, Jokanović V, Mitić-Ćulafić D. Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study. in International Endodontic Journal. 2015;48(10):966-975.
doi:10.1111/iej.12391 .

Petrović, V., Opačić-Galić, Vanja, Živković, Slavoljub, Nikolić, B., Danilović, Vesna, Miletić, Vesna, Jokanović, Vukoman, Mitić-Ćulafić, Dragana, "Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study" in International Endodontic Journal, 48, no. 10 (2015):966-975,
https://doi.org/10.1111/iej.12391 . .

TY  - JOUR
AU  - Petrović, V.
AU  - Opačić-Galić, Vanja
AU  - Živković, Slavoljub
AU  - Nikolić, B.
AU  - Danilović, Vesna
AU  - Miletić, Vesna
AU  - Jokanović, Vukoman
AU  - Mitić-Ćulafić, Dragana
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2012
AB  - AimTo evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems (CS) and hydroxyapatite (HA-CS). MethodologyCytotoxicity of eluates of new nanostructural noncommercial materials CS and HA-CS, and MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil) as a control, were tested using the MTT assay on MRC-5 cells. Eluates of set materials were tested in 100% and 50% concentrations, 24h, 7days and 21days post-elution. The pH values were determined for undiluted eluates of set materials. Polyethylene tubes containing the test materials (CS, HA-CS, MTA) were implanted in subcutaneous tissue of Wistar rats. Histopathological examinations were conducted at 7, 15, 30 and 60days after the implantation. Data were statistically analyzed using three-way and one-way anova Tukey's post hoc test as well as Kruskall-Wallis test with Dunn's post hoc test at =0.05. ResultsAll materials significantly reduced cell viability; especially when undiluted eluates were used (P lt 0.001). After 24h elution, cell viability was 101.8%, 49.5 +/- 4.2% and 61 +/- 7.4%, for MTA, and HA-CS, respectively. However, CS and HA-CS were significantly less toxic than the control material MTA (P lt 0.05). Cytotoxicity could be at least partially attributed to pH kinetics over time. Dilution of eluates of all tested materials resulted in better cell survival. Histopathological examination indicated similar inflammatory reaction, vascular congestion and connective tissue integrity associated with CS, HA-CS and MTA at each observation period (P>0.05). The only significant difference was found for capsule thickness, that is thicker capsule was associated with HA-CS compared to MTA at 60days (P=0.0039). HA-CS induced moderately thick capsules (median score 3, score range 2-3), whereas MTA resulted in thin capsule formation (median score 2, score range 1-3). ConclusionsEvaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil).
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study
VL  - 48
IS  - 10
SP  - 966
EP  - 975
DO  - 10.1111/iej.12391
ER  - 

@article{
author = "Petrović, V. and Opačić-Galić, Vanja and Živković, Slavoljub and Nikolić, B. and Danilović, Vesna and Miletić, Vesna and Jokanović, Vukoman and Mitić-Ćulafić, Dragana",
year = "2015",
abstract = "AimTo evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems (CS) and hydroxyapatite (HA-CS). MethodologyCytotoxicity of eluates of new nanostructural noncommercial materials CS and HA-CS, and MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil) as a control, were tested using the MTT assay on MRC-5 cells. Eluates of set materials were tested in 100% and 50% concentrations, 24h, 7days and 21days post-elution. The pH values were determined for undiluted eluates of set materials. Polyethylene tubes containing the test materials (CS, HA-CS, MTA) were implanted in subcutaneous tissue of Wistar rats. Histopathological examinations were conducted at 7, 15, 30 and 60days after the implantation. Data were statistically analyzed using three-way and one-way anova Tukey's post hoc test as well as Kruskall-Wallis test with Dunn's post hoc test at =0.05. ResultsAll materials significantly reduced cell viability; especially when undiluted eluates were used (P lt 0.001). After 24h elution, cell viability was 101.8%, 49.5 +/- 4.2% and 61 +/- 7.4%, for MTA, and HA-CS, respectively. However, CS and HA-CS were significantly less toxic than the control material MTA (P lt 0.05). Cytotoxicity could be at least partially attributed to pH kinetics over time. Dilution of eluates of all tested materials resulted in better cell survival. Histopathological examination indicated similar inflammatory reaction, vascular congestion and connective tissue integrity associated with CS, HA-CS and MTA at each observation period (P>0.05). The only significant difference was found for capsule thickness, that is thicker capsule was associated with HA-CS compared to MTA at 60days (P=0.0039). HA-CS induced moderately thick capsules (median score 3, score range 2-3), whereas MTA resulted in thin capsule formation (median score 2, score range 1-3). ConclusionsEvaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil).",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study",
volume = "48",
number = "10",
pages = "966-975",
doi = "10.1111/iej.12391"
}

Petrović, V., Opačić-Galić, V., Živković, S., Nikolić, B., Danilović, V., Miletić, V., Jokanović, V.,& Mitić-Ćulafić, D.. (2015). Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study. in International Endodontic Journal
Wiley, Hoboken., 48(10), 966-975.
https://doi.org/10.1111/iej.12391

Petrović V, Opačić-Galić V, Živković S, Nikolić B, Danilović V, Miletić V, Jokanović V, Mitić-Ćulafić D. Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study. in International Endodontic Journal. 2015;48(10):966-975.
doi:10.1111/iej.12391 .

Petrović, V., Opačić-Galić, Vanja, Živković, Slavoljub, Nikolić, B., Danilović, Vesna, Miletić, Vesna, Jokanović, Vukoman, Mitić-Ćulafić, Dragana, "Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study" in International Endodontic Journal, 48, no. 10 (2015):966-975,
https://doi.org/10.1111/iej.12391 . .

TY  - JOUR
AU  - Opačić-Galić, Vanja
AU  - Petrović, V.
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
AU  - Nikolić, B.
AU  - Knežević-Vukcević, Jelena
AU  - Mitić-Ćulafić, Dragana
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1838
AB  - Aim To characterize and investigate the genotoxic effect of a new endodontic cement based on dicalcium- and tricalcium-silicate (CS) with hydroxyapatite (HA) on human lymphocytes. Methodology Hydrothermal treatment was applied for synthesis of CS and HA. The final mixture HA-CS, with potential to be used in endodontic practice, is composed of CS (34%) and HA (66%). Human lymphocytes were incubated with HA, HA-CS and CS for 1h, at 37 degrees C and 5% CO2. Cell viability was determined using the trypan blue exclusion assay. To evaluate the level of DNA damage comet assay (single cell gel electrophoresis) was performed. For the statistical analysis anova and Duncans Post Hoc Test were used. Results The SEM analysis indicated that CS consisted mostly of agglomerates of several micrometers in size, built up from smaller particles, with dimensions between 117 and 477nm. This is promising because dimensions of agglomerates are not comparable with channels inside the cell membranes, whereas their nano-elements provide evident activity, important for faster setting of these mixtures compared to MTA. Values of DNA damage obtained in the comet assay indicated low genotoxic risk of the new endodontic materials. Conclusions The significantly improved setting characteristics and low genotoxic risk of the new material support further research.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes
VL  - 46
IS  - 6
SP  - 506
EP  - 516
DO  - 10.1111/iej.12017
ER  - 

@article{
author = "Opačić-Galić, Vanja and Petrović, V. and Živković, Slavoljub and Jokanović, Vukoman and Nikolić, B. and Knežević-Vukcević, Jelena and Mitić-Ćulafić, Dragana",
year = "2013",
abstract = "Aim To characterize and investigate the genotoxic effect of a new endodontic cement based on dicalcium- and tricalcium-silicate (CS) with hydroxyapatite (HA) on human lymphocytes. Methodology Hydrothermal treatment was applied for synthesis of CS and HA. The final mixture HA-CS, with potential to be used in endodontic practice, is composed of CS (34%) and HA (66%). Human lymphocytes were incubated with HA, HA-CS and CS for 1h, at 37 degrees C and 5% CO2. Cell viability was determined using the trypan blue exclusion assay. To evaluate the level of DNA damage comet assay (single cell gel electrophoresis) was performed. For the statistical analysis anova and Duncans Post Hoc Test were used. Results The SEM analysis indicated that CS consisted mostly of agglomerates of several micrometers in size, built up from smaller particles, with dimensions between 117 and 477nm. This is promising because dimensions of agglomerates are not comparable with channels inside the cell membranes, whereas their nano-elements provide evident activity, important for faster setting of these mixtures compared to MTA. Values of DNA damage obtained in the comet assay indicated low genotoxic risk of the new endodontic materials. Conclusions The significantly improved setting characteristics and low genotoxic risk of the new material support further research.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes",
volume = "46",
number = "6",
pages = "506-516",
doi = "10.1111/iej.12017"
}

Opačić-Galić, V., Petrović, V., Živković, S., Jokanović, V., Nikolić, B., Knežević-Vukcević, J.,& Mitić-Ćulafić, D.. (2013). New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes. in International Endodontic Journal
Wiley, Hoboken., 46(6), 506-516.
https://doi.org/10.1111/iej.12017

Opačić-Galić V, Petrović V, Živković S, Jokanović V, Nikolić B, Knežević-Vukcević J, Mitić-Ćulafić D. New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes. in International Endodontic Journal. 2013;46(6):506-516.
doi:10.1111/iej.12017 .

Opačić-Galić, Vanja, Petrović, V., Živković, Slavoljub, Jokanović, Vukoman, Nikolić, B., Knežević-Vukcević, Jelena, Mitić-Ćulafić, Dragana, "New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes" in International Endodontic Journal, 46, no. 6 (2013):506-516,
https://doi.org/10.1111/iej.12017 . .

TY  - CONF
AU  - Petrović, V.
AU  - Beloica, Dragan
AU  - Vulović, M
AU  - Vulićević, Zoran
PY  - 1997
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1087
PB  - Sage Publications Inc, Thousand Oaks
C3  - Journal of Dental Research
T1  - Clinical trial of contemporary pit and fissure sealants
VL  - 76
SP  - 226
EP  - 226
UR  - https://hdl.handle.net/21.15107/rcub_smile_1087
ER  - 

@conference{
author = "Petrović, V. and Beloica, Dragan and Vulović, M and Vulićević, Zoran",
year = "1997",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Journal of Dental Research",
title = "Clinical trial of contemporary pit and fissure sealants",
volume = "76",
pages = "226-226",
url = "https://hdl.handle.net/21.15107/rcub_smile_1087"
}

Petrović, V., Beloica, D., Vulović, M.,& Vulićević, Z.. (1997). Clinical trial of contemporary pit and fissure sealants. in Journal of Dental Research
Sage Publications Inc, Thousand Oaks., 76, 226-226.
https://hdl.handle.net/21.15107/rcub_smile_1087

Petrović V, Beloica D, Vulović M, Vulićević Z. Clinical trial of contemporary pit and fissure sealants. in Journal of Dental Research. 1997;76:226-226.
https://hdl.handle.net/21.15107/rcub_smile_1087 .

Petrović, V., Beloica, Dragan, Vulović, M, Vulićević, Zoran, "Clinical trial of contemporary pit and fissure sealants" in Journal of Dental Research, 76 (1997):226-226,
https://hdl.handle.net/21.15107/rcub_smile_1087 .

TY  - CONF
AU  - Petrović, V.
AU  - Vulović, M
AU  - Vulićević, Zoran
PY  - 1996
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1068
PB  - Sage Publications Inc, Thousand Oaks
C3  - Journal of Dental Research
T1  - Clinical evaluation of pit and fissure sealants
VL  - 75
SP  - 2153
EP  - 2153
UR  - https://hdl.handle.net/21.15107/rcub_smile_1068
ER  - 

@conference{
author = "Petrović, V. and Vulović, M and Vulićević, Zoran",
year = "1996",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Journal of Dental Research",
title = "Clinical evaluation of pit and fissure sealants",
volume = "75",
pages = "2153-2153",
url = "https://hdl.handle.net/21.15107/rcub_smile_1068"
}

Petrović, V., Vulović, M.,& Vulićević, Z.. (1996). Clinical evaluation of pit and fissure sealants. in Journal of Dental Research
Sage Publications Inc, Thousand Oaks., 75, 2153-2153.
https://hdl.handle.net/21.15107/rcub_smile_1068

Petrović V, Vulović M, Vulićević Z. Clinical evaluation of pit and fissure sealants. in Journal of Dental Research. 1996;75:2153-2153.
https://hdl.handle.net/21.15107/rcub_smile_1068 .

Petrović, V., Vulović, M, Vulićević, Zoran, "Clinical evaluation of pit and fissure sealants" in Journal of Dental Research, 75 (1996):2153-2153,
https://hdl.handle.net/21.15107/rcub_smile_1068 .

TY  - JOUR
AU  - Milašin, Jelena
AU  - Pujić, N.
AU  - Dedović, Nasta
AU  - Nikolić, Ž.
AU  - Petrović, V.
AU  - Dimitrijević, B.
PY  - 1994
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1061
AB  - Nine specimens of well‐differentiated squamous cell carcinoma of the lip vermilion have been analyzed for the pressence of H‐ras oncogene mutations, using the technique of hybridization with synthetic oligonucleotide probes on in vitro amplified tumour DNA. Five specimens harbored mutations: four in codon 12 and one in codon 13. This high incidence (55%) of mutated H‐ras genes suggests that their activation may play an important role in lip tumour development and may be connected to the exposure to chemical and/or physical carcinogens.
T2  - Journal of Oral Pathology & Medicine
T1  - High incidence of H‐ras oncogene mutations in squamous cell carcinoma of lip vermilion
VL  - 23
IS  - 7
SP  - 298
EP  - 301
DO  - 10.1111/j.1600-0714.1994.tb00065.x
ER  - 

@article{
author = "Milašin, Jelena and Pujić, N. and Dedović, Nasta and Nikolić, Ž. and Petrović, V. and Dimitrijević, B.",
year = "1994",
abstract = "Nine specimens of well‐differentiated squamous cell carcinoma of the lip vermilion have been analyzed for the pressence of H‐ras oncogene mutations, using the technique of hybridization with synthetic oligonucleotide probes on in vitro amplified tumour DNA. Five specimens harbored mutations: four in codon 12 and one in codon 13. This high incidence (55%) of mutated H‐ras genes suggests that their activation may play an important role in lip tumour development and may be connected to the exposure to chemical and/or physical carcinogens.",
journal = "Journal of Oral Pathology & Medicine",
title = "High incidence of H‐ras oncogene mutations in squamous cell carcinoma of lip vermilion",
volume = "23",
number = "7",
pages = "298-301",
doi = "10.1111/j.1600-0714.1994.tb00065.x"
}

Milašin, J., Pujić, N., Dedović, N., Nikolić, Ž., Petrović, V.,& Dimitrijević, B.. (1994). High incidence of H‐ras oncogene mutations in squamous cell carcinoma of lip vermilion. in Journal of Oral Pathology & Medicine, 23(7), 298-301.
https://doi.org/10.1111/j.1600-0714.1994.tb00065.x

Milašin J, Pujić N, Dedović N, Nikolić Ž, Petrović V, Dimitrijević B. High incidence of H‐ras oncogene mutations in squamous cell carcinoma of lip vermilion. in Journal of Oral Pathology & Medicine. 1994;23(7):298-301.
doi:10.1111/j.1600-0714.1994.tb00065.x .

Milašin, Jelena, Pujić, N., Dedović, Nasta, Nikolić, Ž., Petrović, V., Dimitrijević, B., "High incidence of H‐ras oncogene mutations in squamous cell carcinoma of lip vermilion" in Journal of Oral Pathology & Medicine, 23, no. 7 (1994):298-301,
https://doi.org/10.1111/j.1600-0714.1994.tb00065.x . .