TY  - JOUR
AU  - Marković, Aleksa
AU  - Mišić, Tijana
AU  - Calvo Guirado, Jose Luis
AU  - Delgado-Ruiz, Rafael
AU  - Janjić, Bojan
AU  - Abboud, Marcus
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2126
AB  - Purpose: To evaluate stability and success rate of hydrophilic nanostructured implants placed via osteotome sinus floor elevation (OSFE) without grafting material or using beta-tricalcium phosphate (beta-TCP), deproteinized bovine bone (DBB), or their combination, and also to assess three-dimensional volumetric stability of endo-sinus bone gained in the aforementioned conditions. Materials and Methods: OSFE with simultaneous implant placement (10-mm long SLActive-BL (R), Straumann, Basel, Switzerland) was performed. Grafting materials were randomly allocated to implant sites, whereas one site was left without graft. Implant stability was measured by resonance frequency analysis over 6 months. Implant success was evaluated after 2 years of loading. Volume of new endo-sinus bone was calculated from CBCT images using 3D Slicer (R) software. Results: A total of 180 implants were inserted into posterior maxilla of 45 patients with 6.59 +/- 0.45 mm of residual bone height, and all remained successful after 2 years. Implant stability steadily increased during healing, without significant difference between groups (p =.658). After 2 years, endo-sinus bone significantly shrank (p  lt .001) in all groups (DBB: 66.34%; beta-TCP: 61.44%; new bone formed from coagulum: 53.02%; beta-TCP + DBB: 33.47%). Conclusions: Endo-sinus bone gained after OSFE inevitably and significantly shrinks regardless of whether grafting material is applied or not. Grafting material offers no significant advantage to stability nor clinical success of hydrophilic and nanostructured implants placed simultaneously with OSFE.
PB  - Wiley, Hoboken
T2  - Clinical Implant Dentistry & Related Research
T1  - Two-Center Prospective, Randomized, Clinical, and Radiographic Study Comparing Osteotome Sinus Floor Elevation with or without Bone Graft and Simultaneous Implant Placement
VL  - 18
IS  - 5
SP  - 873
EP  - 882
DO  - 10.1111/cid.12373
ER  - 

@article{
author = "Marković, Aleksa and Mišić, Tijana and Calvo Guirado, Jose Luis and Delgado-Ruiz, Rafael and Janjić, Bojan and Abboud, Marcus",
year = "2016",
abstract = "Purpose: To evaluate stability and success rate of hydrophilic nanostructured implants placed via osteotome sinus floor elevation (OSFE) without grafting material or using beta-tricalcium phosphate (beta-TCP), deproteinized bovine bone (DBB), or their combination, and also to assess three-dimensional volumetric stability of endo-sinus bone gained in the aforementioned conditions. Materials and Methods: OSFE with simultaneous implant placement (10-mm long SLActive-BL (R), Straumann, Basel, Switzerland) was performed. Grafting materials were randomly allocated to implant sites, whereas one site was left without graft. Implant stability was measured by resonance frequency analysis over 6 months. Implant success was evaluated after 2 years of loading. Volume of new endo-sinus bone was calculated from CBCT images using 3D Slicer (R) software. Results: A total of 180 implants were inserted into posterior maxilla of 45 patients with 6.59 +/- 0.45 mm of residual bone height, and all remained successful after 2 years. Implant stability steadily increased during healing, without significant difference between groups (p =.658). After 2 years, endo-sinus bone significantly shrank (p  lt .001) in all groups (DBB: 66.34%; beta-TCP: 61.44%; new bone formed from coagulum: 53.02%; beta-TCP + DBB: 33.47%). Conclusions: Endo-sinus bone gained after OSFE inevitably and significantly shrinks regardless of whether grafting material is applied or not. Grafting material offers no significant advantage to stability nor clinical success of hydrophilic and nanostructured implants placed simultaneously with OSFE.",
publisher = "Wiley, Hoboken",
journal = "Clinical Implant Dentistry & Related Research",
title = "Two-Center Prospective, Randomized, Clinical, and Radiographic Study Comparing Osteotome Sinus Floor Elevation with or without Bone Graft and Simultaneous Implant Placement",
volume = "18",
number = "5",
pages = "873-882",
doi = "10.1111/cid.12373"
}

Marković, A., Mišić, T., Calvo Guirado, J. L., Delgado-Ruiz, R., Janjić, B.,& Abboud, M.. (2016). Two-Center Prospective, Randomized, Clinical, and Radiographic Study Comparing Osteotome Sinus Floor Elevation with or without Bone Graft and Simultaneous Implant Placement. in Clinical Implant Dentistry & Related Research
Wiley, Hoboken., 18(5), 873-882.
https://doi.org/10.1111/cid.12373

Marković A, Mišić T, Calvo Guirado JL, Delgado-Ruiz R, Janjić B, Abboud M. Two-Center Prospective, Randomized, Clinical, and Radiographic Study Comparing Osteotome Sinus Floor Elevation with or without Bone Graft and Simultaneous Implant Placement. in Clinical Implant Dentistry & Related Research. 2016;18(5):873-882.
doi:10.1111/cid.12373 .

Marković, Aleksa, Mišić, Tijana, Calvo Guirado, Jose Luis, Delgado-Ruiz, Rafael, Janjić, Bojan, Abboud, Marcus, "Two-Center Prospective, Randomized, Clinical, and Radiographic Study Comparing Osteotome Sinus Floor Elevation with or without Bone Graft and Simultaneous Implant Placement" in Clinical Implant Dentistry & Related Research, 18, no. 5 (2016):873-882,
https://doi.org/10.1111/cid.12373 . .

TY  - JOUR
AU  - Delgado-Ruiz, Rafael
AU  - Gomez-Moreno, Gerardo
AU  - Aguilar-Salvatierra, Antonio
AU  - Marković, Aleksa
AU  - Eduardo Mate-Sanchez, Jose
AU  - Calvo Guirado, Jose Luis
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2125
AB  - ObjectivesTo measure the lateral surface area of microgrooved zirconia implants, to evaluate the cell geometry and cell density of human fetal osteoblasts seeded on zirconia microgrooved implants, to describe the surface roughness and chemistry, and to evaluate the activity of human fetal osteoblasts seeded on zirconia microgrooved disks. Materials and methodsThis experimental in vitro study used 62 zirconia implants and 130 zirconia disks. Two experimental groups were created for the implants: 31 non-microgrooved implants (Control) and 31 microgrooved implants (Test); two experimental groups were created for the disks: 65 non-microgrooved disks (Control) and 65 microgrooved disks (Test). The following evaluations of the implants were made: lateral surface area (LSA), cell morphology, and density of human fetal osteoblasts seeded on implant surfaces. On the disks, surface parameters (roughness and chemistry) and cell activity (alkaline phosphatase - ALP and alizarin red - ALZ) were evaluated at 7 and 15days. ResultsLSA was lower for control implants (62.8mm) compared with test implants (128.74mm) (P lt 0.05). Cell bodies on control surfaces were flattened and disorganized, while in the test group, they were aligned inside the microgrooves. Control group cells showed few lamellipodia, which were attached mainly inside topographical accidents (surface cracks, valleys, and pits). Test group implants presented cells rich in lamellipodia prolongations, attached to the inner walls or to the borders of the microgrooves and in the flat areas between the microgrooves. Cell density was higher in the test group compared with controls (P lt 0.05) Surface roughness and oxygen content increased in test disks samples compared with controls (P lt 0.05). Carbon and aluminum were reduced in disks test samples compared with controls (P lt 0.05), and ALP and ALZ levels were significantly increased on test surfaces (P lt 0.05) at both study times. ConclusionsWithin the limitations of this experimental study, it may be concluded that (i) Roughness is increased and chemical composition enhanced on the surface of zirconia implants with microgrooves. (ii) The LSA of microgrooved zirconia implants is greater and provides more available surface compared with implants of the same dimensions without microgrooves. (iii) Microgrooves on zirconia implants modify the morphology and guide the size and alignment of human fetal osteoblasts. (iv) Zirconia surfaces with microgrooves of 30m width and 70m separation between grooves enhance ALP and ALZ expression by human fetal osteoblasts.
PB  - Wiley, Hoboken
T2  - Clinical Oral Implants Research
T1  - Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study
VL  - 27
IS  - 11
SP  - e144
EP  - e153
DO  - 10.1111/clr.12585
ER  - 

@article{
author = "Delgado-Ruiz, Rafael and Gomez-Moreno, Gerardo and Aguilar-Salvatierra, Antonio and Marković, Aleksa and Eduardo Mate-Sanchez, Jose and Calvo Guirado, Jose Luis",
year = "2016",
abstract = "ObjectivesTo measure the lateral surface area of microgrooved zirconia implants, to evaluate the cell geometry and cell density of human fetal osteoblasts seeded on zirconia microgrooved implants, to describe the surface roughness and chemistry, and to evaluate the activity of human fetal osteoblasts seeded on zirconia microgrooved disks. Materials and methodsThis experimental in vitro study used 62 zirconia implants and 130 zirconia disks. Two experimental groups were created for the implants: 31 non-microgrooved implants (Control) and 31 microgrooved implants (Test); two experimental groups were created for the disks: 65 non-microgrooved disks (Control) and 65 microgrooved disks (Test). The following evaluations of the implants were made: lateral surface area (LSA), cell morphology, and density of human fetal osteoblasts seeded on implant surfaces. On the disks, surface parameters (roughness and chemistry) and cell activity (alkaline phosphatase - ALP and alizarin red - ALZ) were evaluated at 7 and 15days. ResultsLSA was lower for control implants (62.8mm) compared with test implants (128.74mm) (P lt 0.05). Cell bodies on control surfaces were flattened and disorganized, while in the test group, they were aligned inside the microgrooves. Control group cells showed few lamellipodia, which were attached mainly inside topographical accidents (surface cracks, valleys, and pits). Test group implants presented cells rich in lamellipodia prolongations, attached to the inner walls or to the borders of the microgrooves and in the flat areas between the microgrooves. Cell density was higher in the test group compared with controls (P lt 0.05) Surface roughness and oxygen content increased in test disks samples compared with controls (P lt 0.05). Carbon and aluminum were reduced in disks test samples compared with controls (P lt 0.05), and ALP and ALZ levels were significantly increased on test surfaces (P lt 0.05) at both study times. ConclusionsWithin the limitations of this experimental study, it may be concluded that (i) Roughness is increased and chemical composition enhanced on the surface of zirconia implants with microgrooves. (ii) The LSA of microgrooved zirconia implants is greater and provides more available surface compared with implants of the same dimensions without microgrooves. (iii) Microgrooves on zirconia implants modify the morphology and guide the size and alignment of human fetal osteoblasts. (iv) Zirconia surfaces with microgrooves of 30m width and 70m separation between grooves enhance ALP and ALZ expression by human fetal osteoblasts.",
publisher = "Wiley, Hoboken",
journal = "Clinical Oral Implants Research",
title = "Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study",
volume = "27",
number = "11",
pages = "e144-e153",
doi = "10.1111/clr.12585"
}

Delgado-Ruiz, R., Gomez-Moreno, G., Aguilar-Salvatierra, A., Marković, A., Eduardo Mate-Sanchez, J.,& Calvo Guirado, J. L.. (2016). Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study. in Clinical Oral Implants Research
Wiley, Hoboken., 27(11), e144-e153.
https://doi.org/10.1111/clr.12585

Delgado-Ruiz R, Gomez-Moreno G, Aguilar-Salvatierra A, Marković A, Eduardo Mate-Sanchez J, Calvo Guirado JL. Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study. in Clinical Oral Implants Research. 2016;27(11):e144-e153.
doi:10.1111/clr.12585 .

Delgado-Ruiz, Rafael, Gomez-Moreno, Gerardo, Aguilar-Salvatierra, Antonio, Marković, Aleksa, Eduardo Mate-Sanchez, Jose, Calvo Guirado, Jose Luis, "Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study" in Clinical Oral Implants Research, 27, no. 11 (2016):e144-e153,
https://doi.org/10.1111/clr.12585 . .

TY  - JOUR
AU  - Delgado-Ruiz, Rafael
AU  - Calvo Guirado, Jose Luis
AU  - Abboud, Marcus
AU  - Piedad Ramirez-Fernandez, Maria
AU  - Eduardo Mate-Sanchez, Jose
AU  - Negri, Bruno
AU  - Gomez-Moreno, Gerardo
AU  - Marković, Aleksa
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1970
AB  - PurposeTo describe contact, thickness, density, and orientation of connective tissue fibers around healing abutments of different geometries by means of a new method using coordinates. Materials and MethodsFollowing the bilateral extraction of mandibular premolars (P2, P3, and P4) from six fox hound dogs and a 2-month healing period, 36 titanium implants were inserted, onto which two groups of healing abutments of different geometry were screwed: Group A (concave abutments) and Group B (wider healing abutment). After 3 months the animals were sacrificed and samples extracted containing each implant and surrounding soft and hard tissues. Histological analysis was performed without decalcifying the samples by means of circularly polarized light under optical microscope and a system of vertical and horizontal coordinates across all the connective tissue in an area delimited by the implant/abutment, epithelium, and bone tissue. ResultsIn no case had the connective tissue formed a connection to the healing abutment/implant in the internal zone; a space of 3510m separated the connective tissue fibers from the healing abutment surface. The total thickness of connective tissue in the horizontal direction was significantly greater in the medial zone in Group B than in Group A (p lt .05). The orientation of the fibers varied according to the coordinate area so that internal coordinates showed a higher percentage of parallel fibers in Group A (p lt .05) and a higher percentage of oblique fibers in Group B (p lt .05); medial coordinates showed more oblique fibers (p lt .05); and the area of external coordinates showed the highest percentage of perpendicular fibers (p lt .05). The fiber density was higher in the basal and medial areas (p lt .05). ConclusionsAbutment geometry influences the orientation of collagen fibers; therefore, an abutment with a profile wider than the implant platform favors oblique and perpendicular orientation of collagen fibers and greater connective tissue thickness.
PB  - Wiley, Hoboken
T2  - Clinical Implant Dentistry & Related Research
T1  - Connective Tissue Characteristics around Healing Abutments of Different Geometries: New Methodological Technique under Circularly Polarized Light
VL  - 17
IS  - 4
SP  - 667
EP  - 680
DO  - 10.1111/cid.12161
ER  - 

@article{
author = "Delgado-Ruiz, Rafael and Calvo Guirado, Jose Luis and Abboud, Marcus and Piedad Ramirez-Fernandez, Maria and Eduardo Mate-Sanchez, Jose and Negri, Bruno and Gomez-Moreno, Gerardo and Marković, Aleksa",
year = "2015",
abstract = "PurposeTo describe contact, thickness, density, and orientation of connective tissue fibers around healing abutments of different geometries by means of a new method using coordinates. Materials and MethodsFollowing the bilateral extraction of mandibular premolars (P2, P3, and P4) from six fox hound dogs and a 2-month healing period, 36 titanium implants were inserted, onto which two groups of healing abutments of different geometry were screwed: Group A (concave abutments) and Group B (wider healing abutment). After 3 months the animals were sacrificed and samples extracted containing each implant and surrounding soft and hard tissues. Histological analysis was performed without decalcifying the samples by means of circularly polarized light under optical microscope and a system of vertical and horizontal coordinates across all the connective tissue in an area delimited by the implant/abutment, epithelium, and bone tissue. ResultsIn no case had the connective tissue formed a connection to the healing abutment/implant in the internal zone; a space of 3510m separated the connective tissue fibers from the healing abutment surface. The total thickness of connective tissue in the horizontal direction was significantly greater in the medial zone in Group B than in Group A (p lt .05). The orientation of the fibers varied according to the coordinate area so that internal coordinates showed a higher percentage of parallel fibers in Group A (p lt .05) and a higher percentage of oblique fibers in Group B (p lt .05); medial coordinates showed more oblique fibers (p lt .05); and the area of external coordinates showed the highest percentage of perpendicular fibers (p lt .05). The fiber density was higher in the basal and medial areas (p lt .05). ConclusionsAbutment geometry influences the orientation of collagen fibers; therefore, an abutment with a profile wider than the implant platform favors oblique and perpendicular orientation of collagen fibers and greater connective tissue thickness.",
publisher = "Wiley, Hoboken",
journal = "Clinical Implant Dentistry & Related Research",
title = "Connective Tissue Characteristics around Healing Abutments of Different Geometries: New Methodological Technique under Circularly Polarized Light",
volume = "17",
number = "4",
pages = "667-680",
doi = "10.1111/cid.12161"
}

Delgado-Ruiz, R., Calvo Guirado, J. L., Abboud, M., Piedad Ramirez-Fernandez, M., Eduardo Mate-Sanchez, J., Negri, B., Gomez-Moreno, G.,& Marković, A.. (2015). Connective Tissue Characteristics around Healing Abutments of Different Geometries: New Methodological Technique under Circularly Polarized Light. in Clinical Implant Dentistry & Related Research
Wiley, Hoboken., 17(4), 667-680.
https://doi.org/10.1111/cid.12161

Delgado-Ruiz R, Calvo Guirado JL, Abboud M, Piedad Ramirez-Fernandez M, Eduardo Mate-Sanchez J, Negri B, Gomez-Moreno G, Marković A. Connective Tissue Characteristics around Healing Abutments of Different Geometries: New Methodological Technique under Circularly Polarized Light. in Clinical Implant Dentistry & Related Research. 2015;17(4):667-680.
doi:10.1111/cid.12161 .

Delgado-Ruiz, Rafael, Calvo Guirado, Jose Luis, Abboud, Marcus, Piedad Ramirez-Fernandez, Maria, Eduardo Mate-Sanchez, Jose, Negri, Bruno, Gomez-Moreno, Gerardo, Marković, Aleksa, "Connective Tissue Characteristics around Healing Abutments of Different Geometries: New Methodological Technique under Circularly Polarized Light" in Clinical Implant Dentistry & Related Research, 17, no. 4 (2015):667-680,
https://doi.org/10.1111/cid.12161 . .

TY  - JOUR
AU  - Delgado-Ruiz, Rafael
AU  - Marković, Aleksa
AU  - Calvo Guirado, Jose Luis
AU  - Lazić, Zoran
AU  - Piattelli, Adriano
AU  - Boticelli, Daniele
AU  - Maté-Sánchez, José Eduardo
AU  - Negri, Bruno
AU  - Ramírez-Fernández, María Piedad
AU  - Mišić, Tijana
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1900
AB  - Background/Aim. The modification of implant surfaces could affect mechanical implant stability as well as dynamics and quality of peri-implant bone healing. The aim of this 3-month experimental study in dogs was to investigate implant stability, marginal bone levels and bone tissue response to zirconia dental implants with two laser-micro-grooved intraosseous surfaces in comparison with nongrooved sandblasted zirconia and sandblasted, high-temperature etched titanium implants. Methods. Implant surface characterization was performed using optical interferometric profilometry and energy dispersive X-ray spectroscopy. A total of 96 implants (4 mm in diameter and 10 mm in length) were inserted randomly in both sides of the lower jaw of 12 Fox Hound dogs divided into groups of 24 each: the control (titanium), the group A (sandblasted zirconia), the group B (sandblasted zirconia plus microgrooved neck) and the group C (sandblasted zirconia plus all microgrooved). All the implants were immediately loaded. Insertion torque, periotest values, radiographic crestal bone level and removal torque were recorded during the 3-month follow-up. Qualitative scanning electon microscope (SEM) analysis of the bone-implant interfaces of each group was performed. Results. Insertion torque values were higher in the group C and control implants (p  lt  0.05). Periotest values increased in all the periods in proportion to the extent of microgrooving as follows: the group C > the control > the group B > the group A (p  lt  0.05). Radiographic measurements showed minimal crestal bone loss at 3 months for microgrooved zirconia implants (groups C and B) and control implants compared with the group A implants (p  lt  0.05). The removal torque values increased with time for all the groups as follows: the group C > the control > the group B > the group A (p  lt  0.05). SEM showed that implant surfaces of the groups B and C had an extra bone growth inside the microgrooves that corresponded to the shape and direction of the microgrooves. Conclusion. The addition of microgrooves to the entire intraosseous surface of zirconia dental implants enhances primary and secondary implant stability, promotes bone tissue in growth and preserves crestal bone levels.
AB  - Uvod/Cilj. Modifikacija površine implantata može uticati na njegovu mehaničku stabilnost kao i na dinamiku i kvalitet periimplantatnog koštanog zarastanja. Cilj ove tromesečne eksperimentalne studije na psima bio je da se ispita stabilnost implantata, nivo marginalne kosti i odgovor koštanog tkiva na cirkonijum endoosealne implantate sa dve intraosealne površine mikrostrukturirane laserom u poređenju sa peskiranim cirkonijum implantatima čija površina nije mikrostrukturirana kao i sa titanijum implantatima čije su površine peskirane i nagrižene visokom temperaturom. Metode. Karakterizacija površine implantata učinjena je optičkom interferometrijskom profilometrijom i analizom energetskog spektra pri difrakciji X-zračenja. Ukupno 96 implantata (prečnika 4 mm i dužine 10 mm) ugrađeno je nasumično i obostrano u donju vilicu kod 12 pasa (lisičara) i podeljeno u četiri grupe po 24: kontrolna (titanijum implantati); grupa A (peskirani cirkonijum implantati); grupa B (peskirani cirkonijum implantati sa mikrokanalima u koronarnoj trećini); grupa C (peskirani cirkonijum implantati sa mikrokanalima duž cele površine). Svi implantati su odmah opterećeni. Meren je obrtni momenat pri ugradnji implantata, vrednosti periotesta, radiografski nivo marginalne kosti i obrtni moment za uklanjanje implantata tokom tromesečnog perioda praćenja. Međuspoj kosti i implantata iz svake grupe ispitivan je kvalitativnom skenirajućom elektronskom mikroskopijom (SEM). Rezultati. Veći obrtni momenat zabeležen je pri ugradnji implantata kod grupe C i kontrolne grupe (p  lt  0,05). U ispitivanom vremenskom periodu, vrednosti periotesta uvećavale su se srazmerno obimu mikrostrukturiranja površine i to: grupa C > kontrolna grupa > grupa B > grupa A (p  lt  0,05). Radiografskom analizom utvrđen je minimalni gubitak marginalne kosti u trećem mesecu praćenja oko cirkonijum implantata sa mikrokanalima (grupa B i C) i kontrola u poređenju sa implantatima grupe A (p  lt  0,05). Vrednosti obrtnog momenta za uklanjanje implantata vremenom su se uvećavale u svim grupama na sledeći način: grupa C > kontrolna grupa > grupa B > grupa A (p  lt  0,05). Kod implantatnih površina grupa B i C, SEM je pokazala dodatni rast koštanog tkiva unutar mikrokanala koji odgovara njihovom obliku i pravcu. Zaključak. Formiranje mikrokanala duž cele intraosealne površine cirkonijum endoosealnih implantata povećava primarnu i sekundarnu implantatnu stabilnost, podstiče urastanje koštanog tkiva i održava nivo marginalne kosti.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Implant stability and marginal bone level of microgrooved zirconia dental implants: A 3-month experimental study on dogs
T1  - Implantatna stabilnost i nivo marginalne kosti kod cirkonijum endoosealnih implantata sa mikrostrukturiranom površinom - tromesečna eksperimentalna studija na psima
VL  - 71
IS  - 5
SP  - 451
EP  - 461
DO  - 10.2298/VSP121003034D
ER  - 

@article{
author = "Delgado-Ruiz, Rafael and Marković, Aleksa and Calvo Guirado, Jose Luis and Lazić, Zoran and Piattelli, Adriano and Boticelli, Daniele and Maté-Sánchez, José Eduardo and Negri, Bruno and Ramírez-Fernández, María Piedad and Mišić, Tijana",
year = "2014",
abstract = "Background/Aim. The modification of implant surfaces could affect mechanical implant stability as well as dynamics and quality of peri-implant bone healing. The aim of this 3-month experimental study in dogs was to investigate implant stability, marginal bone levels and bone tissue response to zirconia dental implants with two laser-micro-grooved intraosseous surfaces in comparison with nongrooved sandblasted zirconia and sandblasted, high-temperature etched titanium implants. Methods. Implant surface characterization was performed using optical interferometric profilometry and energy dispersive X-ray spectroscopy. A total of 96 implants (4 mm in diameter and 10 mm in length) were inserted randomly in both sides of the lower jaw of 12 Fox Hound dogs divided into groups of 24 each: the control (titanium), the group A (sandblasted zirconia), the group B (sandblasted zirconia plus microgrooved neck) and the group C (sandblasted zirconia plus all microgrooved). All the implants were immediately loaded. Insertion torque, periotest values, radiographic crestal bone level and removal torque were recorded during the 3-month follow-up. Qualitative scanning electon microscope (SEM) analysis of the bone-implant interfaces of each group was performed. Results. Insertion torque values were higher in the group C and control implants (p  lt  0.05). Periotest values increased in all the periods in proportion to the extent of microgrooving as follows: the group C > the control > the group B > the group A (p  lt  0.05). Radiographic measurements showed minimal crestal bone loss at 3 months for microgrooved zirconia implants (groups C and B) and control implants compared with the group A implants (p  lt  0.05). The removal torque values increased with time for all the groups as follows: the group C > the control > the group B > the group A (p  lt  0.05). SEM showed that implant surfaces of the groups B and C had an extra bone growth inside the microgrooves that corresponded to the shape and direction of the microgrooves. Conclusion. The addition of microgrooves to the entire intraosseous surface of zirconia dental implants enhances primary and secondary implant stability, promotes bone tissue in growth and preserves crestal bone levels., Uvod/Cilj. Modifikacija površine implantata može uticati na njegovu mehaničku stabilnost kao i na dinamiku i kvalitet periimplantatnog koštanog zarastanja. Cilj ove tromesečne eksperimentalne studije na psima bio je da se ispita stabilnost implantata, nivo marginalne kosti i odgovor koštanog tkiva na cirkonijum endoosealne implantate sa dve intraosealne površine mikrostrukturirane laserom u poređenju sa peskiranim cirkonijum implantatima čija površina nije mikrostrukturirana kao i sa titanijum implantatima čije su površine peskirane i nagrižene visokom temperaturom. Metode. Karakterizacija površine implantata učinjena je optičkom interferometrijskom profilometrijom i analizom energetskog spektra pri difrakciji X-zračenja. Ukupno 96 implantata (prečnika 4 mm i dužine 10 mm) ugrađeno je nasumično i obostrano u donju vilicu kod 12 pasa (lisičara) i podeljeno u četiri grupe po 24: kontrolna (titanijum implantati); grupa A (peskirani cirkonijum implantati); grupa B (peskirani cirkonijum implantati sa mikrokanalima u koronarnoj trećini); grupa C (peskirani cirkonijum implantati sa mikrokanalima duž cele površine). Svi implantati su odmah opterećeni. Meren je obrtni momenat pri ugradnji implantata, vrednosti periotesta, radiografski nivo marginalne kosti i obrtni moment za uklanjanje implantata tokom tromesečnog perioda praćenja. Međuspoj kosti i implantata iz svake grupe ispitivan je kvalitativnom skenirajućom elektronskom mikroskopijom (SEM). Rezultati. Veći obrtni momenat zabeležen je pri ugradnji implantata kod grupe C i kontrolne grupe (p  lt  0,05). U ispitivanom vremenskom periodu, vrednosti periotesta uvećavale su se srazmerno obimu mikrostrukturiranja površine i to: grupa C > kontrolna grupa > grupa B > grupa A (p  lt  0,05). Radiografskom analizom utvrđen je minimalni gubitak marginalne kosti u trećem mesecu praćenja oko cirkonijum implantata sa mikrokanalima (grupa B i C) i kontrola u poređenju sa implantatima grupe A (p  lt  0,05). Vrednosti obrtnog momenta za uklanjanje implantata vremenom su se uvećavale u svim grupama na sledeći način: grupa C > kontrolna grupa > grupa B > grupa A (p  lt  0,05). Kod implantatnih površina grupa B i C, SEM je pokazala dodatni rast koštanog tkiva unutar mikrokanala koji odgovara njihovom obliku i pravcu. Zaključak. Formiranje mikrokanala duž cele intraosealne površine cirkonijum endoosealnih implantata povećava primarnu i sekundarnu implantatnu stabilnost, podstiče urastanje koštanog tkiva i održava nivo marginalne kosti.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Implant stability and marginal bone level of microgrooved zirconia dental implants: A 3-month experimental study on dogs, Implantatna stabilnost i nivo marginalne kosti kod cirkonijum endoosealnih implantata sa mikrostrukturiranom površinom - tromesečna eksperimentalna studija na psima",
volume = "71",
number = "5",
pages = "451-461",
doi = "10.2298/VSP121003034D"
}

Delgado-Ruiz, R., Marković, A., Calvo Guirado, J. L., Lazić, Z., Piattelli, A., Boticelli, D., Maté-Sánchez, J. E., Negri, B., Ramírez-Fernández, M. P.,& Mišić, T.. (2014). Implant stability and marginal bone level of microgrooved zirconia dental implants: A 3-month experimental study on dogs. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 71(5), 451-461.
https://doi.org/10.2298/VSP121003034D

Delgado-Ruiz R, Marković A, Calvo Guirado JL, Lazić Z, Piattelli A, Boticelli D, Maté-Sánchez JE, Negri B, Ramírez-Fernández MP, Mišić T. Implant stability and marginal bone level of microgrooved zirconia dental implants: A 3-month experimental study on dogs. in Vojnosanitetski pregled. 2014;71(5):451-461.
doi:10.2298/VSP121003034D .

Delgado-Ruiz, Rafael, Marković, Aleksa, Calvo Guirado, Jose Luis, Lazić, Zoran, Piattelli, Adriano, Boticelli, Daniele, Maté-Sánchez, José Eduardo, Negri, Bruno, Ramírez-Fernández, María Piedad, Mišić, Tijana, "Implant stability and marginal bone level of microgrooved zirconia dental implants: A 3-month experimental study on dogs" in Vojnosanitetski pregled, 71, no. 5 (2014):451-461,
https://doi.org/10.2298/VSP121003034D . .

TY  - JOUR
AU  - Marković, Aleksa
AU  - Luis Calvo-Guirado, Jose
AU  - Lazić, Zoran
AU  - Gomez-Moreno, Gerardo
AU  - Ćalasan, Dejan
AU  - Guardia, Javier
AU  - Čolić, Snježana
AU  - Aguilar-Salvatierra, Antonio
AU  - Gačić, Bojan
AU  - Delgado-Ruiz, Rafael
AU  - Janjić, Bojan
AU  - Mišić, Tijana
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1816
AB  - Purpose: The aim of this study was to investigate the relationship between surgical techniques and implant macro-design (self-tapping/non-self-tapping) for the optimization of implant stability in the low-density bone present in the posterior maxilla using resonance frequency analysis (RFA). Materials and Methods: A total of 102 implants were studied. Fifty-six self-tapping BlueSkyBredent (R) (Bredent GmbH &, Senden, Germany) and 56 non-self-tapping Standard Plus Straumann (R) (Institut Straumann AG (R), Waldenburg, Switzerland) were placed in the posterior segment of the maxilla. Implants of both types were placed in sites prepared with either lateral bone-condensing or with bone-drilling techniques. Implant stability measurements were performed using RFA immediately after implant placement and weekly during a 12-week follow-up period. Results: Both types of implants placed after bone condensing achieved significantly higher stability immediately after surgery, as well as during the entire 12-week observation period compared with those placed following bone drilling. After bone condensation, there were no significant differences in primary stability or in implant stability after the first week between both implant types. From 2 to 12 postoperative weeks, significantly higher stability was shown by self-tapping implants. After bone drilling, self-tapping implants achieved significantly higher stability than non-self-tapping implants during the entire follow-up period. Conclusions: The outcomes of the present study indicate that bone drilling is not an effective technique for improving implant stability and, following this technique, the use of self-tapping implants is highly recommended. Implant stability optimization in the soft bone can be achieved by lateral bone-condensing technique, regardless of implant macro-design.
PB  - Wiley, Hoboken
T2  - Clinical Implant Dentistry & Related Research
T1  - Evaluation of Primary Stability of Self-Tapping and Non-Self-Tapping Dental Implants. A 12-Week Clinical Study
VL  - 15
IS  - 3
SP  - 341
EP  - 349
DO  - 10.1111/j.1708-8208.2011.00415.x
ER  - 

@article{
author = "Marković, Aleksa and Luis Calvo-Guirado, Jose and Lazić, Zoran and Gomez-Moreno, Gerardo and Ćalasan, Dejan and Guardia, Javier and Čolić, Snježana and Aguilar-Salvatierra, Antonio and Gačić, Bojan and Delgado-Ruiz, Rafael and Janjić, Bojan and Mišić, Tijana",
year = "2013",
abstract = "Purpose: The aim of this study was to investigate the relationship between surgical techniques and implant macro-design (self-tapping/non-self-tapping) for the optimization of implant stability in the low-density bone present in the posterior maxilla using resonance frequency analysis (RFA). Materials and Methods: A total of 102 implants were studied. Fifty-six self-tapping BlueSkyBredent (R) (Bredent GmbH &, Senden, Germany) and 56 non-self-tapping Standard Plus Straumann (R) (Institut Straumann AG (R), Waldenburg, Switzerland) were placed in the posterior segment of the maxilla. Implants of both types were placed in sites prepared with either lateral bone-condensing or with bone-drilling techniques. Implant stability measurements were performed using RFA immediately after implant placement and weekly during a 12-week follow-up period. Results: Both types of implants placed after bone condensing achieved significantly higher stability immediately after surgery, as well as during the entire 12-week observation period compared with those placed following bone drilling. After bone condensation, there were no significant differences in primary stability or in implant stability after the first week between both implant types. From 2 to 12 postoperative weeks, significantly higher stability was shown by self-tapping implants. After bone drilling, self-tapping implants achieved significantly higher stability than non-self-tapping implants during the entire follow-up period. Conclusions: The outcomes of the present study indicate that bone drilling is not an effective technique for improving implant stability and, following this technique, the use of self-tapping implants is highly recommended. Implant stability optimization in the soft bone can be achieved by lateral bone-condensing technique, regardless of implant macro-design.",
publisher = "Wiley, Hoboken",
journal = "Clinical Implant Dentistry & Related Research",
title = "Evaluation of Primary Stability of Self-Tapping and Non-Self-Tapping Dental Implants. A 12-Week Clinical Study",
volume = "15",
number = "3",
pages = "341-349",
doi = "10.1111/j.1708-8208.2011.00415.x"
}

Marković, A., Luis Calvo-Guirado, J., Lazić, Z., Gomez-Moreno, G., Ćalasan, D., Guardia, J., Čolić, S., Aguilar-Salvatierra, A., Gačić, B., Delgado-Ruiz, R., Janjić, B.,& Mišić, T.. (2013). Evaluation of Primary Stability of Self-Tapping and Non-Self-Tapping Dental Implants. A 12-Week Clinical Study. in Clinical Implant Dentistry & Related Research
Wiley, Hoboken., 15(3), 341-349.
https://doi.org/10.1111/j.1708-8208.2011.00415.x

Marković A, Luis Calvo-Guirado J, Lazić Z, Gomez-Moreno G, Ćalasan D, Guardia J, Čolić S, Aguilar-Salvatierra A, Gačić B, Delgado-Ruiz R, Janjić B, Mišić T. Evaluation of Primary Stability of Self-Tapping and Non-Self-Tapping Dental Implants. A 12-Week Clinical Study. in Clinical Implant Dentistry & Related Research. 2013;15(3):341-349.
doi:10.1111/j.1708-8208.2011.00415.x .

Marković, Aleksa, Luis Calvo-Guirado, Jose, Lazić, Zoran, Gomez-Moreno, Gerardo, Ćalasan, Dejan, Guardia, Javier, Čolić, Snježana, Aguilar-Salvatierra, Antonio, Gačić, Bojan, Delgado-Ruiz, Rafael, Janjić, Bojan, Mišić, Tijana, "Evaluation of Primary Stability of Self-Tapping and Non-Self-Tapping Dental Implants. A 12-Week Clinical Study" in Clinical Implant Dentistry & Related Research, 15, no. 3 (2013):341-349,
https://doi.org/10.1111/j.1708-8208.2011.00415.x . .

TY  - JOUR
AU  - Šćepanović, Miodrag
AU  - Luis Calvo-Guirado, Jose
AU  - Marković, Aleksa
AU  - Delgado-Ruiz, Rafael
AU  - Todorović, Aleksandar
AU  - Miličić, Biljana
AU  - Mišić, Tijana
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1687
AB  - Aim: The aim of this 1-year prospective cohort study was to evaluate the outcomes and complications of immediately loaded mini dental implants used for stabilizing mandibular overdentures in edentulous patients wearing conventional complete dentures. Materials and methods: Thirty patients received mandibular conventional complete dentures that were subsequently retained by 4 immediately loaded mini dental implants placed in the interforaminal region. Overdenture success, implant success and biological and prosthetic complications were evaluated after the first year of service. Quality of life using the Oral Heath Impact Profile (OHIP)-EDENT test, satisfaction with dental prosthesis and chewing efficiency using questionnaires and a Visual Analogue Scale (VAS) were evaluated twice: after they had received mandibular complete dentures, and again after they had received mandibular overdentures. Results: After 1 year, no overdenture failed and 2 implants did not osseointegrate, resulting in a 98.3% success rate for loaded implants and 95.9% for total implants used (3 out of 123 were not loaded due to fracture). A flap surgical approach was performed in 7 patients, 3 implants fractured during insertion, 3 overdentures fractured, occlusion balancing was necessary for 11 dentures and relining for 8. Implant rehabilitation provided significant improvement in quality of life, stability, comfort, chewing and speaking ability while no significant differences were found in quality of maintenance of hygiene and for aesthetics. Conclusions: Mini dental implant retained overdentures can be a successful therapeutic procedure for treating mandibular edentulism that improves quality of life, patient satisfaction and chewing ability in patients wearing maxillary dentures. Longer follow-ups are needed to validate this therapy in the medium and long-term.
PB  - Quintessence Publishing Co., Ltd
T2  - European Journal of Oral Implantology
T1  - A 1-year prospective cohort study on mandibular overdentures retained by mini dental implants
VL  - 5
IS  - 4
SP  - 367
EP  - 379
UR  - https://hdl.handle.net/21.15107/rcub_smile_1687
ER  - 

@article{
author = "Šćepanović, Miodrag and Luis Calvo-Guirado, Jose and Marković, Aleksa and Delgado-Ruiz, Rafael and Todorović, Aleksandar and Miličić, Biljana and Mišić, Tijana",
year = "2012",
abstract = "Aim: The aim of this 1-year prospective cohort study was to evaluate the outcomes and complications of immediately loaded mini dental implants used for stabilizing mandibular overdentures in edentulous patients wearing conventional complete dentures. Materials and methods: Thirty patients received mandibular conventional complete dentures that were subsequently retained by 4 immediately loaded mini dental implants placed in the interforaminal region. Overdenture success, implant success and biological and prosthetic complications were evaluated after the first year of service. Quality of life using the Oral Heath Impact Profile (OHIP)-EDENT test, satisfaction with dental prosthesis and chewing efficiency using questionnaires and a Visual Analogue Scale (VAS) were evaluated twice: after they had received mandibular complete dentures, and again after they had received mandibular overdentures. Results: After 1 year, no overdenture failed and 2 implants did not osseointegrate, resulting in a 98.3% success rate for loaded implants and 95.9% for total implants used (3 out of 123 were not loaded due to fracture). A flap surgical approach was performed in 7 patients, 3 implants fractured during insertion, 3 overdentures fractured, occlusion balancing was necessary for 11 dentures and relining for 8. Implant rehabilitation provided significant improvement in quality of life, stability, comfort, chewing and speaking ability while no significant differences were found in quality of maintenance of hygiene and for aesthetics. Conclusions: Mini dental implant retained overdentures can be a successful therapeutic procedure for treating mandibular edentulism that improves quality of life, patient satisfaction and chewing ability in patients wearing maxillary dentures. Longer follow-ups are needed to validate this therapy in the medium and long-term.",
publisher = "Quintessence Publishing Co., Ltd",
journal = "European Journal of Oral Implantology",
title = "A 1-year prospective cohort study on mandibular overdentures retained by mini dental implants",
volume = "5",
number = "4",
pages = "367-379",
url = "https://hdl.handle.net/21.15107/rcub_smile_1687"
}

Šćepanović, M., Luis Calvo-Guirado, J., Marković, A., Delgado-Ruiz, R., Todorović, A., Miličić, B.,& Mišić, T.. (2012). A 1-year prospective cohort study on mandibular overdentures retained by mini dental implants. in European Journal of Oral Implantology
Quintessence Publishing Co., Ltd., 5(4), 367-379.
https://hdl.handle.net/21.15107/rcub_smile_1687

Šćepanović M, Luis Calvo-Guirado J, Marković A, Delgado-Ruiz R, Todorović A, Miličić B, Mišić T. A 1-year prospective cohort study on mandibular overdentures retained by mini dental implants. in European Journal of Oral Implantology. 2012;5(4):367-379.
https://hdl.handle.net/21.15107/rcub_smile_1687 .

Šćepanović, Miodrag, Luis Calvo-Guirado, Jose, Marković, Aleksa, Delgado-Ruiz, Rafael, Todorović, Aleksandar, Miličić, Biljana, Mišić, Tijana, "A 1-year prospective cohort study on mandibular overdentures retained by mini dental implants" in European Journal of Oral Implantology, 5, no. 4 (2012):367-379,
https://hdl.handle.net/21.15107/rcub_smile_1687 .