TY  - JOUR
AU  - Trivanović, Drenka
AU  - Jauković, Aleksandra
AU  - Popović, Branka
AU  - Krstić, Jelena
AU  - Mojsilović, Slavko
AU  - Okic-Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Santibanez, Juan Francisco
AU  - Bugarski, Diana
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2018
AB  - Aims: In vitro expansion changes replication and differentiation capacity of mesenchymal stem cells (MSCs), increasing challenges and risks, while limiting the sufficient number of MSCs required for cytotherapy. Here, we characterized and compared proliferation, differentiation, telomere length and pluripotency marker expression in MSCs of various origins. Main methods: Immunophenotyping, proliferation and differentiation assays were performed. Pluripotency marker (Nanog, Oct-4, SOX-2, SSEA-4) expression was determined by immunofluorescence. Quantitative PCR was performed for relative telomere length (RTL) analyses, while expression of relevant genes for pluripotency markers, differentiation state (Cbfa1, human placental alkaline phosphatase, peroxisome proliferator activated receptor, Sox9 and Collagen II a1), and telomerase reverse transcriptase (hTERT) was determined by semiquantitative RT-PCR. Key findings: Peripheral blood MSCs (PB-MSCs) and umbilical cord MSCs (UC-MSCs) showed the highest, while periodontal ligament MSCs (PDL-MSCs) and adipose tissue MSCs (AT-MSCs) the lowest values of both the replication potential and RTL. Although MSCs from exfoliated deciduous teeth (SHEDs), PDL-MSCs and AT-MSCs showed higher mRNA expression of pluripotency markers, all MSCs expressed pluripotency marker proteins. SHEDs and PDL-MSCs showed prominent capacity for osteogenesis, PB-MSCs and UC-MSCs showed strengthened adipogenic differentiation potential, while AT-MSCs displayed similar differentiation into both lines. Significance: The MSCs populations derived from different sources, although displaying similar phenotype, exhibited high degree of variability regarding biological properties related to their self-renewal and differentiation capacity. These data indicate that for more accurate use in cell therapy, individualities of MSCs isolated from different tissues should be identified and taken into consideration when planning their use in clinical protocols.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression
VL  - 141
SP  - 61
EP  - 73
DO  - 10.1016/j.lfs.2015.09.019
ER  - 

@article{
author = "Trivanović, Drenka and Jauković, Aleksandra and Popović, Branka and Krstić, Jelena and Mojsilović, Slavko and Okic-Đorđević, Ivana and Kukolj, Tamara and Obradović, Hristina and Santibanez, Juan Francisco and Bugarski, Diana",
year = "2015",
abstract = "Aims: In vitro expansion changes replication and differentiation capacity of mesenchymal stem cells (MSCs), increasing challenges and risks, while limiting the sufficient number of MSCs required for cytotherapy. Here, we characterized and compared proliferation, differentiation, telomere length and pluripotency marker expression in MSCs of various origins. Main methods: Immunophenotyping, proliferation and differentiation assays were performed. Pluripotency marker (Nanog, Oct-4, SOX-2, SSEA-4) expression was determined by immunofluorescence. Quantitative PCR was performed for relative telomere length (RTL) analyses, while expression of relevant genes for pluripotency markers, differentiation state (Cbfa1, human placental alkaline phosphatase, peroxisome proliferator activated receptor, Sox9 and Collagen II a1), and telomerase reverse transcriptase (hTERT) was determined by semiquantitative RT-PCR. Key findings: Peripheral blood MSCs (PB-MSCs) and umbilical cord MSCs (UC-MSCs) showed the highest, while periodontal ligament MSCs (PDL-MSCs) and adipose tissue MSCs (AT-MSCs) the lowest values of both the replication potential and RTL. Although MSCs from exfoliated deciduous teeth (SHEDs), PDL-MSCs and AT-MSCs showed higher mRNA expression of pluripotency markers, all MSCs expressed pluripotency marker proteins. SHEDs and PDL-MSCs showed prominent capacity for osteogenesis, PB-MSCs and UC-MSCs showed strengthened adipogenic differentiation potential, while AT-MSCs displayed similar differentiation into both lines. Significance: The MSCs populations derived from different sources, although displaying similar phenotype, exhibited high degree of variability regarding biological properties related to their self-renewal and differentiation capacity. These data indicate that for more accurate use in cell therapy, individualities of MSCs isolated from different tissues should be identified and taken into consideration when planning their use in clinical protocols.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression",
volume = "141",
pages = "61-73",
doi = "10.1016/j.lfs.2015.09.019"
}

Trivanović, D., Jauković, A., Popović, B., Krstić, J., Mojsilović, S., Okic-Đorđević, I., Kukolj, T., Obradović, H., Santibanez, J. F.,& Bugarski, D.. (2015). Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 141, 61-73.
https://doi.org/10.1016/j.lfs.2015.09.019

Trivanović D, Jauković A, Popović B, Krstić J, Mojsilović S, Okic-Đorđević I, Kukolj T, Obradović H, Santibanez JF, Bugarski D. Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression. in Life Sciences. 2015;141:61-73.
doi:10.1016/j.lfs.2015.09.019 .

Trivanović, Drenka, Jauković, Aleksandra, Popović, Branka, Krstić, Jelena, Mojsilović, Slavko, Okic-Đorđević, Ivana, Kukolj, Tamara, Obradović, Hristina, Santibanez, Juan Francisco, Bugarski, Diana, "Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression" in Life Sciences, 141 (2015):61-73,
https://doi.org/10.1016/j.lfs.2015.09.019 . .