Obradović, Radmila

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Antimicrobial activity of essential oils against oral pathogens: Informative article

Stanković, Ivana; Kesić, Ljiljana; Milašin, Jelena; Obradović, Radmila; Petrović, Milica S.; Bojović, Marija

(Univerzitet u Nišu - Medicinski fakultet, Niš, 2018)

TY  - JOUR
AU  - Stanković, Ivana
AU  - Kesić, Ljiljana
AU  - Milašin, Jelena
AU  - Obradović, Radmila
AU  - Petrović, Milica S.
AU  - Bojović, Marija
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2338
AB  - Periodontal disease and Dental caries associated with dental plaque are the most common bacterial diseases, but also, significant oral health problem is Candidiasis. Candida albicans, is an opportunistic pathogen that can, under certain conditions proliferate and cause infections. The need for prevention and alternative forms of treatment and products for oral diseases comes from the rise in disease incidence, increased resistance by pathogenic bacteria to currently used chemotherapeutics. The products derived from medicinal plants have proven to be a source of biologically active substances, and thanks to their active principles, products based on medical herbs are more prevalent in modern phytotherapy. Essential oils are complex natural mixtures of volatile secondary metabolites - aliphatic and aromatic, terpinen and phenyl- propane compounds isolated from plants. The main constituents of essential oils are terpenes and sesquiterpenes including carbohydrates, alcohols, ethers, aldehydes and ketones, which are responsible for the fragrant and biological properties of plants. Different oils produce various pharmacological effects such as anti-inflammatory, antioxidant and anticancerogenic properties, but also oils are biocides. There are numerous in vitro studies that dealt with the research activities of natural herbal substances against oral bacteria that are known to be etiological factors in the development of oral and dental diseases. The phenolic major compounds of essential oils have been suggested to have a potential antifungal activity. There is ample of evidence that plant extracts and essential oils have the potential to be developed into agents that can be used as preventative or treatment therapies of oral diseases.
AB  - Parodontopatija i dentalni karijes, čiji je nastanak povezan sa dentalnim plakom, predstavljaju najčešća bakterijska oboljenja. Takođe, značajni oralni zdravstveni problem predstavlja i kandidijaza. Candida albicans je oportunistički patogen koji pod pojedinim okolnostima može da proliferiše i uzrokuje infekciju. Potreba za prevencijom i alternativnim oblicima lečenja i produktima za oralna oboljenja proizilazi zbog povećanja učestalosti oboljenja i povećane rezistencije patogenih bakterija na primenu hemoterapeutika koji se trenutno upotrebljavaju. Dokazano je da su produkti dobijeni od lekovitog bilja izvor biološko aktivnih supstanci, a zahvaljujući svojim aktivnim principima, produkti na bazi lekovitog bilja zastupljeniji su u modernoj fitoterapiji. Eterična ulja su kompleksne prirodne mešavine isparljivih sekundarnih metabolita - alifatičnih i aromatičnih, terpenskih i fenil-propanskih jedinjenja izolovanih iz biljaka. Glavni sastojci etarskih ulja su terpeni i seskviterpeni, uključujući i ugljene hidrate, alkohol, etar, aldehide i ketone, koji su odgovorni za mirisna i biološka svojstva biljaka. Različita ulja imaju brojne farmakološke efekte, kao što su antiinflamatorno, antioksidativno i antikancerogeno dejstvo, ali su takođe i biocidi. Postoje brojne in vitro studije koje su se bavile istraživanjem aktivnosti prirodnih biljnih supstanci usmerenih na oralne bakterije za koje je poznato da su etiološki faktori u nastanku oralnih i dentalnih oboljenja. Navedeno je da glavne fenolne komponente eteričnih ulja imaju potencijalnu antigljivičnu aktivnost. Postoje brojni dokazi da biljni ekstrakti i eterična ulja imaju potencijal da se razviju u agense koji se mogu koristiti u preventivi ili u lečenju oralnih oboljenja.
PB  - Univerzitet u Nišu - Medicinski fakultet, Niš
T2  - Acta medica Medianae
T1  - Antimicrobial activity of essential oils against oral pathogens: Informative article
T1  - Antimikrobno dejstvo eteričnih ulja na oralne patogene
VL  - 57
IS  - 3
SP  - 115
EP  - 123
DO  - 10.5633/amm.2018.0316
ER  - 
@article{
author = "Stanković, Ivana and Kesić, Ljiljana and Milašin, Jelena and Obradović, Radmila and Petrović, Milica S. and Bojović, Marija",
year = "2018",
abstract = "Periodontal disease and Dental caries associated with dental plaque are the most common bacterial diseases, but also, significant oral health problem is Candidiasis. Candida albicans, is an opportunistic pathogen that can, under certain conditions proliferate and cause infections. The need for prevention and alternative forms of treatment and products for oral diseases comes from the rise in disease incidence, increased resistance by pathogenic bacteria to currently used chemotherapeutics. The products derived from medicinal plants have proven to be a source of biologically active substances, and thanks to their active principles, products based on medical herbs are more prevalent in modern phytotherapy. Essential oils are complex natural mixtures of volatile secondary metabolites - aliphatic and aromatic, terpinen and phenyl- propane compounds isolated from plants. The main constituents of essential oils are terpenes and sesquiterpenes including carbohydrates, alcohols, ethers, aldehydes and ketones, which are responsible for the fragrant and biological properties of plants. Different oils produce various pharmacological effects such as anti-inflammatory, antioxidant and anticancerogenic properties, but also oils are biocides. There are numerous in vitro studies that dealt with the research activities of natural herbal substances against oral bacteria that are known to be etiological factors in the development of oral and dental diseases. The phenolic major compounds of essential oils have been suggested to have a potential antifungal activity. There is ample of evidence that plant extracts and essential oils have the potential to be developed into agents that can be used as preventative or treatment therapies of oral diseases., Parodontopatija i dentalni karijes, čiji je nastanak povezan sa dentalnim plakom, predstavljaju najčešća bakterijska oboljenja. Takođe, značajni oralni zdravstveni problem predstavlja i kandidijaza. Candida albicans je oportunistički patogen koji pod pojedinim okolnostima može da proliferiše i uzrokuje infekciju. Potreba za prevencijom i alternativnim oblicima lečenja i produktima za oralna oboljenja proizilazi zbog povećanja učestalosti oboljenja i povećane rezistencije patogenih bakterija na primenu hemoterapeutika koji se trenutno upotrebljavaju. Dokazano je da su produkti dobijeni od lekovitog bilja izvor biološko aktivnih supstanci, a zahvaljujući svojim aktivnim principima, produkti na bazi lekovitog bilja zastupljeniji su u modernoj fitoterapiji. Eterična ulja su kompleksne prirodne mešavine isparljivih sekundarnih metabolita - alifatičnih i aromatičnih, terpenskih i fenil-propanskih jedinjenja izolovanih iz biljaka. Glavni sastojci etarskih ulja su terpeni i seskviterpeni, uključujući i ugljene hidrate, alkohol, etar, aldehide i ketone, koji su odgovorni za mirisna i biološka svojstva biljaka. Različita ulja imaju brojne farmakološke efekte, kao što su antiinflamatorno, antioksidativno i antikancerogeno dejstvo, ali su takođe i biocidi. Postoje brojne in vitro studije koje su se bavile istraživanjem aktivnosti prirodnih biljnih supstanci usmerenih na oralne bakterije za koje je poznato da su etiološki faktori u nastanku oralnih i dentalnih oboljenja. Navedeno je da glavne fenolne komponente eteričnih ulja imaju potencijalnu antigljivičnu aktivnost. Postoje brojni dokazi da biljni ekstrakti i eterična ulja imaju potencijal da se razviju u agense koji se mogu koristiti u preventivi ili u lečenju oralnih oboljenja.",
publisher = "Univerzitet u Nišu - Medicinski fakultet, Niš",
journal = "Acta medica Medianae",
title = "Antimicrobial activity of essential oils against oral pathogens: Informative article, Antimikrobno dejstvo eteričnih ulja na oralne patogene",
volume = "57",
number = "3",
pages = "115-123",
doi = "10.5633/amm.2018.0316"
}
Stanković, I., Kesić, L., Milašin, J., Obradović, R., Petrović, M. S.,& Bojović, M.. (2018). Antimicrobial activity of essential oils against oral pathogens: Informative article. in Acta medica Medianae
Univerzitet u Nišu - Medicinski fakultet, Niš., 57(3), 115-123.
https://doi.org/10.5633/amm.2018.0316
Stanković I, Kesić L, Milašin J, Obradović R, Petrović MS, Bojović M. Antimicrobial activity of essential oils against oral pathogens: Informative article. in Acta medica Medianae. 2018;57(3):115-123.
doi:10.5633/amm.2018.0316 .
Stanković, Ivana, Kesić, Ljiljana, Milašin, Jelena, Obradović, Radmila, Petrović, Milica S., Bojović, Marija, "Antimicrobial activity of essential oils against oral pathogens: Informative article" in Acta medica Medianae, 57, no. 3 (2018):115-123,
https://doi.org/10.5633/amm.2018.0316 . .

Clinical, microbiological and cytomorphometric evaluation of low-level laser therapy as an adjunct to periodontal therapy in patients with chronic periodontitis

Petrović, M. S.; Kannosh, Ibrahim; Milašin, Jelena; Mihailović, D. S.; Obradović, Radmila; Bubanj, S. R.; Kesić, Ljiljana

(Wiley, Hoboken, 2018)

TY  - JOUR
AU  - Petrović, M. S.
AU  - Kannosh, Ibrahim
AU  - Milašin, Jelena
AU  - Mihailović, D. S.
AU  - Obradović, Radmila
AU  - Bubanj, S. R.
AU  - Kesić, Ljiljana
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2286
AB  - ObjectiveA clinical prospective study was designed to evaluate microbiological, cytomorphometric and clinical efficacy of low-level laser therapy (LLLT) as an adjunct to periodontal therapy in the treatment of chronic periodontitis. MethodsSixty subjects were included and randomly assigned into 2 groups: SRP (scaling root planning) group (n=30) and LLLT+SRP group (n=30). Clinical parameters were measured before intervention, after the fifth treatment, and after a month. All subjects received oral hygiene instructions and full-mouth conservative periodontal treatment (removal of dental plaque followed by SRP). Afterwards, in group II, Kavo LLLT (980nm, 0.2W, 6J/cm(2)) was applied. Subgingival samples were collected at baseline and after the fifth treatment to quantify Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythensis and Treponema denticola by polymerase chain reaction (PCR). Gingival swabs were taken, and direct smears were prepared on slides for cytomorphometric analysis. ResultsEvaluation using clinical parameters showed better results in LLLT group. A statistically significant decrease in the prevalence of bacteria after treatment in LLLT group was observed for the following: T.forsythensis and T.denticola (P lt .001), P.gingivalis (P lt .01), A.actinomycetemcomitans and P.intermedia (P lt .05). The values of nuclear area, perimeter and Ferret's diameter were significantly lower in both studied groups after treatment, but statistical significance was higher in LLLT group (P lt .001) than in the SRP therapy group (P lt .05). ConclusionLow-level laser therapy as an adjunct to periodontal therapy demonstrates short-term additional bacteriological, cytological and clinical benefits.
PB  - Wiley, Hoboken
T2  - International Journal of Dental Hygiene
T1  - Clinical, microbiological and cytomorphometric evaluation of low-level laser therapy as an adjunct to periodontal therapy in patients with chronic periodontitis
VL  - 16
IS  - 2
SP  - E120
EP  - E127
DO  - 10.1111/idh.12328
ER  - 
@article{
author = "Petrović, M. S. and Kannosh, Ibrahim and Milašin, Jelena and Mihailović, D. S. and Obradović, Radmila and Bubanj, S. R. and Kesić, Ljiljana",
year = "2018",
abstract = "ObjectiveA clinical prospective study was designed to evaluate microbiological, cytomorphometric and clinical efficacy of low-level laser therapy (LLLT) as an adjunct to periodontal therapy in the treatment of chronic periodontitis. MethodsSixty subjects were included and randomly assigned into 2 groups: SRP (scaling root planning) group (n=30) and LLLT+SRP group (n=30). Clinical parameters were measured before intervention, after the fifth treatment, and after a month. All subjects received oral hygiene instructions and full-mouth conservative periodontal treatment (removal of dental plaque followed by SRP). Afterwards, in group II, Kavo LLLT (980nm, 0.2W, 6J/cm(2)) was applied. Subgingival samples were collected at baseline and after the fifth treatment to quantify Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythensis and Treponema denticola by polymerase chain reaction (PCR). Gingival swabs were taken, and direct smears were prepared on slides for cytomorphometric analysis. ResultsEvaluation using clinical parameters showed better results in LLLT group. A statistically significant decrease in the prevalence of bacteria after treatment in LLLT group was observed for the following: T.forsythensis and T.denticola (P lt .001), P.gingivalis (P lt .01), A.actinomycetemcomitans and P.intermedia (P lt .05). The values of nuclear area, perimeter and Ferret's diameter were significantly lower in both studied groups after treatment, but statistical significance was higher in LLLT group (P lt .001) than in the SRP therapy group (P lt .05). ConclusionLow-level laser therapy as an adjunct to periodontal therapy demonstrates short-term additional bacteriological, cytological and clinical benefits.",
publisher = "Wiley, Hoboken",
journal = "International Journal of Dental Hygiene",
title = "Clinical, microbiological and cytomorphometric evaluation of low-level laser therapy as an adjunct to periodontal therapy in patients with chronic periodontitis",
volume = "16",
number = "2",
pages = "E120-E127",
doi = "10.1111/idh.12328"
}
Petrović, M. S., Kannosh, I., Milašin, J., Mihailović, D. S., Obradović, R., Bubanj, S. R.,& Kesić, L.. (2018). Clinical, microbiological and cytomorphometric evaluation of low-level laser therapy as an adjunct to periodontal therapy in patients with chronic periodontitis. in International Journal of Dental Hygiene
Wiley, Hoboken., 16(2), E120-E127.
https://doi.org/10.1111/idh.12328
Petrović MS, Kannosh I, Milašin J, Mihailović DS, Obradović R, Bubanj SR, Kesić L. Clinical, microbiological and cytomorphometric evaluation of low-level laser therapy as an adjunct to periodontal therapy in patients with chronic periodontitis. in International Journal of Dental Hygiene. 2018;16(2):E120-E127.
doi:10.1111/idh.12328 .
Petrović, M. S., Kannosh, Ibrahim, Milašin, Jelena, Mihailović, D. S., Obradović, Radmila, Bubanj, S. R., Kesić, Ljiljana, "Clinical, microbiological and cytomorphometric evaluation of low-level laser therapy as an adjunct to periodontal therapy in patients with chronic periodontitis" in International Journal of Dental Hygiene, 16, no. 2 (2018):E120-E127,
https://doi.org/10.1111/idh.12328 . .
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Microbial etiology of periodontal disease: Mini review

Kesić, Ljiljana; Milašin, Jelena; Igić, Marija; Obradović, Radmila

(Univerzitet u Nišu, Niš, 2008)

TY  - JOUR
AU  - Kesić, Ljiljana
AU  - Milašin, Jelena
AU  - Igić, Marija
AU  - Obradović, Radmila
PY  - 2008
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1380
AB  - The periodontal disease is a chronic, degenerative disease which is localised on the gingiva, periodontal ligament, cementum and alveolar bone. The main etiological factor is oral biofilm with microorganisms. The search for the pathogens of periodontal diseases has been underway for more than 100 years, and continues up today. The currently recognized key Gram negative periodontopathogens include: Porphyromonas gingivalis, Prevotella intermedia, Bacteroides forsythus, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Capnocytophaga species, Campylobacter rectus.All bacteria in the periodontal pocket could damage periodontal tissues, and good knowledge of these as well as an adequate treatment could be helpful in treatment of this disease. A full understanding of the microbial factors, their pathogenicity as well as host factors are of the essential importance for pathogenesis of periodontal disease. In this way, it could be possible to treat the periodontal patients adequately.
AB  - Parodontopatija predstavlja hroničnu, destruktivnu bolest, koja zahvata čitav potporni aparat zuba (gingiva, cement, alveolarna kost i periodoncijum). Glavni etiološki faktor u nastanku ovog oboljenja je oralni biofilm sa mikroorganizmima. Poslednjih 100 godina istraživači ispituju sastav mikrobiološke flore parodontalnog džepa. Do danas ključni Gram negativni periodontopatogeni su: Porphyromonas gingivalis, Prevotella intermedia, Bacteroides forsythus, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Capnocytophaga species, Campylobacter rectus. Sve prisutne bakterije u parodontalnom džepu mogu oštetiti parodontalna tkiva, i dobro poznavanje mikroorganizama omogućava i adekvatan tretman parodontopatije. Potpuno poznavanje mikroroganizama, njihove patogenosti, kao i poznavanje odgovora domaćina su osnovni za razumevanje patogeneze parodontopatije. ovo bi omogućilo i lečenje pacijenata sa parodontopatijom na odgovarajući način.
PB  - Univerzitet u Nišu, Niš
T2  - Facta universitatis - series: Medicine & Biology
T1  - Microbial etiology of periodontal disease: Mini review
T1  - Mikrobiološka etiologija parodontopatije
VL  - 15
IS  - 1
SP  - 1
EP  - 6
UR  - https://hdl.handle.net/21.15107/rcub_smile_1380
ER  - 
@article{
author = "Kesić, Ljiljana and Milašin, Jelena and Igić, Marija and Obradović, Radmila",
year = "2008",
abstract = "The periodontal disease is a chronic, degenerative disease which is localised on the gingiva, periodontal ligament, cementum and alveolar bone. The main etiological factor is oral biofilm with microorganisms. The search for the pathogens of periodontal diseases has been underway for more than 100 years, and continues up today. The currently recognized key Gram negative periodontopathogens include: Porphyromonas gingivalis, Prevotella intermedia, Bacteroides forsythus, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Capnocytophaga species, Campylobacter rectus.All bacteria in the periodontal pocket could damage periodontal tissues, and good knowledge of these as well as an adequate treatment could be helpful in treatment of this disease. A full understanding of the microbial factors, their pathogenicity as well as host factors are of the essential importance for pathogenesis of periodontal disease. In this way, it could be possible to treat the periodontal patients adequately., Parodontopatija predstavlja hroničnu, destruktivnu bolest, koja zahvata čitav potporni aparat zuba (gingiva, cement, alveolarna kost i periodoncijum). Glavni etiološki faktor u nastanku ovog oboljenja je oralni biofilm sa mikroorganizmima. Poslednjih 100 godina istraživači ispituju sastav mikrobiološke flore parodontalnog džepa. Do danas ključni Gram negativni periodontopatogeni su: Porphyromonas gingivalis, Prevotella intermedia, Bacteroides forsythus, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Capnocytophaga species, Campylobacter rectus. Sve prisutne bakterije u parodontalnom džepu mogu oštetiti parodontalna tkiva, i dobro poznavanje mikroorganizama omogućava i adekvatan tretman parodontopatije. Potpuno poznavanje mikroroganizama, njihove patogenosti, kao i poznavanje odgovora domaćina su osnovni za razumevanje patogeneze parodontopatije. ovo bi omogućilo i lečenje pacijenata sa parodontopatijom na odgovarajući način.",
publisher = "Univerzitet u Nišu, Niš",
journal = "Facta universitatis - series: Medicine & Biology",
title = "Microbial etiology of periodontal disease: Mini review, Mikrobiološka etiologija parodontopatije",
volume = "15",
number = "1",
pages = "1-6",
url = "https://hdl.handle.net/21.15107/rcub_smile_1380"
}
Kesić, L., Milašin, J., Igić, M.,& Obradović, R.. (2008). Microbial etiology of periodontal disease: Mini review. in Facta universitatis - series: Medicine & Biology
Univerzitet u Nišu, Niš., 15(1), 1-6.
https://hdl.handle.net/21.15107/rcub_smile_1380
Kesić L, Milašin J, Igić M, Obradović R. Microbial etiology of periodontal disease: Mini review. in Facta universitatis - series: Medicine & Biology. 2008;15(1):1-6.
https://hdl.handle.net/21.15107/rcub_smile_1380 .
Kesić, Ljiljana, Milašin, Jelena, Igić, Marija, Obradović, Radmila, "Microbial etiology of periodontal disease: Mini review" in Facta universitatis - series: Medicine & Biology, 15, no. 1 (2008):1-6,
https://hdl.handle.net/21.15107/rcub_smile_1380 .