TY  - JOUR
AU  - Stojić, Vanja
AU  - Glišić, Branislav
AU  - Đukić, Ljiljana
AU  - Prokić, Bogomir
AU  - Janović, Aleksa
AU  - Stamenković, Zorana
AU  - Milutinović-Smiljanić, Sanja
AU  - Danilović, Vesna
AU  - Brković, Božidar
AU  - Roganović, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2512
AB  - Objective: We aimed to investigate alteration in cellular signaling mediated by vascular endothelial growth factor (VEGF) and parameters of oxidative stress/nitric oxide generation, superoxide dismutase (SOD) and neuronal nitric oxide synthase (nNOS), underlying altered functional mechanical loading of TMJ (temporomandibular joint) during lateral mandibular deviation. Design: Thirty-eight 5-week-old male Wistar rats were divided into experimental group, which received acrylic resin appliance that shifted mandible to the left during closure, and control group. Computed tomography and histomorphometry were used for condyle analyses, while samples of condyle, synovial membrane and m. masseter were analyzed with enzyme-linked immunosorbent assay and spectrophotometry to determine VEGF and nNOS protein concentrations, and SOD activity. Results: Experimental group of rats developed smaller and asymmetrical mandibles. Less of new bone and cartilage formation and larger bone marrow cavities area were found in the experimental group. Higher VEGF expression in condyle and m. masseter as well as higher nNOS expression in m. masseter and synovial membrane were found in the experimental compared to the control group. Alteration of SOD activity was found in m. masseter and synovial membrane in the experimental group. Conclusions: Lateral mandibular deviation induces mandibular and condylar morphological changes as well as significant cellular signaling alterations in condyle, synovial membrane and masticatory muscle. Cellular VEGF protein overexpression and oxidative stress/nitric oxide disbalance could be the mechanisms underlying unbalanced functional TMJ loading due to mandibular deviation.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Archives of Oral Biology
T1  - Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle
VL  - 110
DO  - 10.1016/j.archoralbio.2019.104599
ER  - 

@article{
author = "Stojić, Vanja and Glišić, Branislav and Đukić, Ljiljana and Prokić, Bogomir and Janović, Aleksa and Stamenković, Zorana and Milutinović-Smiljanić, Sanja and Danilović, Vesna and Brković, Božidar and Roganović, Jelena",
year = "2020",
abstract = "Objective: We aimed to investigate alteration in cellular signaling mediated by vascular endothelial growth factor (VEGF) and parameters of oxidative stress/nitric oxide generation, superoxide dismutase (SOD) and neuronal nitric oxide synthase (nNOS), underlying altered functional mechanical loading of TMJ (temporomandibular joint) during lateral mandibular deviation. Design: Thirty-eight 5-week-old male Wistar rats were divided into experimental group, which received acrylic resin appliance that shifted mandible to the left during closure, and control group. Computed tomography and histomorphometry were used for condyle analyses, while samples of condyle, synovial membrane and m. masseter were analyzed with enzyme-linked immunosorbent assay and spectrophotometry to determine VEGF and nNOS protein concentrations, and SOD activity. Results: Experimental group of rats developed smaller and asymmetrical mandibles. Less of new bone and cartilage formation and larger bone marrow cavities area were found in the experimental group. Higher VEGF expression in condyle and m. masseter as well as higher nNOS expression in m. masseter and synovial membrane were found in the experimental compared to the control group. Alteration of SOD activity was found in m. masseter and synovial membrane in the experimental group. Conclusions: Lateral mandibular deviation induces mandibular and condylar morphological changes as well as significant cellular signaling alterations in condyle, synovial membrane and masticatory muscle. Cellular VEGF protein overexpression and oxidative stress/nitric oxide disbalance could be the mechanisms underlying unbalanced functional TMJ loading due to mandibular deviation.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Archives of Oral Biology",
title = "Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle",
volume = "110",
doi = "10.1016/j.archoralbio.2019.104599"
}

Stojić, V., Glišić, B., Đukić, L., Prokić, B., Janović, A., Stamenković, Z., Milutinović-Smiljanić, S., Danilović, V., Brković, B.,& Roganović, J.. (2020). Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle. in Archives of Oral Biology
Pergamon-Elsevier Science Ltd, Oxford., 110.
https://doi.org/10.1016/j.archoralbio.2019.104599

Stojić V, Glišić B, Đukić L, Prokić B, Janović A, Stamenković Z, Milutinović-Smiljanić S, Danilović V, Brković B, Roganović J. Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle. in Archives of Oral Biology. 2020;110.
doi:10.1016/j.archoralbio.2019.104599 .

Stojić, Vanja, Glišić, Branislav, Đukić, Ljiljana, Prokić, Bogomir, Janović, Aleksa, Stamenković, Zorana, Milutinović-Smiljanić, Sanja, Danilović, Vesna, Brković, Božidar, Roganović, Jelena, "Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle" in Archives of Oral Biology, 110 (2020),
https://doi.org/10.1016/j.archoralbio.2019.104599 . .

TY  - JOUR
AU  - Bajić, Dragana
AU  - Skorić, Tamara
AU  - Milutinović-Smiljanić, Sanja
AU  - Japundžić-Žigon, Nina
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2418
AB  - This paper proposes a method that maps the coupling strength of an arbitrary number of signals D, D >= 2, into a single time series. It is motivated by the inability of multiscale entropy to jointly analyze more than two signals. The coupling strength is determined using the copula density defined over a [0 1](D) copula domain. The copula domain is decomposed into the Voronoi regions, with volumes inversely proportional to the dependency level (coupling strength) of the observed joint signals. A stream of dependency levels, ordered in time, creates a new time series that shows the fluctuation of the signals' coupling strength along the time axis. The composite multiscale entropy (CMSE) is then applied to three signals, systolic blood pressure (SBP), pulse interval (PI), and body temperature (t(B)), simultaneously recorded from rats exposed to different ambient temperatures (t(A)). The obtained results are consistent with the results from the classical studies, and the method itself offers more levels of freedom than the classical analysis.
PB  - Mdpi, Basel
T2  - Entropy
T1  - Voronoi Decomposition of Cardiovascular Dependency Structures in Different Ambient Conditions: An Entropy Study
VL  - 21
IS  - 11
DO  - 10.3390/e21111103
ER  - 

@article{
author = "Bajić, Dragana and Skorić, Tamara and Milutinović-Smiljanić, Sanja and Japundžić-Žigon, Nina",
year = "2019",
abstract = "This paper proposes a method that maps the coupling strength of an arbitrary number of signals D, D >= 2, into a single time series. It is motivated by the inability of multiscale entropy to jointly analyze more than two signals. The coupling strength is determined using the copula density defined over a [0 1](D) copula domain. The copula domain is decomposed into the Voronoi regions, with volumes inversely proportional to the dependency level (coupling strength) of the observed joint signals. A stream of dependency levels, ordered in time, creates a new time series that shows the fluctuation of the signals' coupling strength along the time axis. The composite multiscale entropy (CMSE) is then applied to three signals, systolic blood pressure (SBP), pulse interval (PI), and body temperature (t(B)), simultaneously recorded from rats exposed to different ambient temperatures (t(A)). The obtained results are consistent with the results from the classical studies, and the method itself offers more levels of freedom than the classical analysis.",
publisher = "Mdpi, Basel",
journal = "Entropy",
title = "Voronoi Decomposition of Cardiovascular Dependency Structures in Different Ambient Conditions: An Entropy Study",
volume = "21",
number = "11",
doi = "10.3390/e21111103"
}

Bajić, D., Skorić, T., Milutinović-Smiljanić, S.,& Japundžić-Žigon, N.. (2019). Voronoi Decomposition of Cardiovascular Dependency Structures in Different Ambient Conditions: An Entropy Study. in Entropy
Mdpi, Basel., 21(11).
https://doi.org/10.3390/e21111103

Bajić D, Skorić T, Milutinović-Smiljanić S, Japundžić-Žigon N. Voronoi Decomposition of Cardiovascular Dependency Structures in Different Ambient Conditions: An Entropy Study. in Entropy. 2019;21(11).
doi:10.3390/e21111103 .

Bajić, Dragana, Skorić, Tamara, Milutinović-Smiljanić, Sanja, Japundžić-Žigon, Nina, "Voronoi Decomposition of Cardiovascular Dependency Structures in Different Ambient Conditions: An Entropy Study" in Entropy, 21, no. 11 (2019),
https://doi.org/10.3390/e21111103 . .

TY  - JOUR
AU  - Jovanović, Slađana
AU  - Skorić, Tamara
AU  - Sarenac, Olivera
AU  - Milutinović-Smiljanić, Sanja
AU  - Japundžić-Žigon, Nina
AU  - Bajić, Dragana
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2291
AB  - Objectives: Copula is a tool for measuring linear and non-linear interactions between two or more time series. The aim of this paper is to prove that a copula approach can accurately capture and visualize the spatial and temporal fluctuations in dependency structures of cardiovascular signals, and to outline the application possibilities. Methods: The method for measuring the level of interaction between systolic blood pressure and the corresponding pulse interval is validated statistically and pharmacologically. The time series are recorded from the freely moving male Wistar rats equipped with radio-telemetry device for blood pressure recording, before and after administration of autonomic blockers scopolamine, atenolol, prazosin and hexamethonium. Implicit (Gaussian and t) and explicit (Clayton, Frank and Gumbel) copulas were calculated and compared to the conventional bivariate methods (Kendal, Pearson, Spearman and classical correlation). Further statistical validation was done using artificially generated surrogate data. A window sliding procedure for dynamic monitoring the signals' coupling strength is implemented. Results: Under the baseline physiological conditions, SBP-PI dependency is significant for time lags 0 s-4 s. Hexamethonium completely abolished the dependency, scopolamine abolished it for time lags 0 s-2 s, atenolol first slightly increased, than for lags greater than 2 s decreased the dependency and prazosin had no effect. Isospectral and isodistributional surrogate data tests confirm that copulas successfully notify the absence of dependency as well. Conclusion: Copula approach accurately captures the temporal fluctuations in dependency structures of SBP and PI, simultaneously enabling a visualization of dependency levels within the particular signal zones. An analysis showed that copulas are more sensitive than the conventional statistical measures, with Frank copula exhibiting the best characterization of SBP and PI dependency.
PB  - Elsevier Sci Ltd, Oxford
T2  - Biomedical Signal Processing & Control
T1  - Copula as a dynamic measure of cardiovascular signal interactions
VL  - 43
SP  - 250
EP  - 264
DO  - 10.1016/j.bspc.2018.03.007
ER  - 

@article{
author = "Jovanović, Slađana and Skorić, Tamara and Sarenac, Olivera and Milutinović-Smiljanić, Sanja and Japundžić-Žigon, Nina and Bajić, Dragana",
year = "2018",
abstract = "Objectives: Copula is a tool for measuring linear and non-linear interactions between two or more time series. The aim of this paper is to prove that a copula approach can accurately capture and visualize the spatial and temporal fluctuations in dependency structures of cardiovascular signals, and to outline the application possibilities. Methods: The method for measuring the level of interaction between systolic blood pressure and the corresponding pulse interval is validated statistically and pharmacologically. The time series are recorded from the freely moving male Wistar rats equipped with radio-telemetry device for blood pressure recording, before and after administration of autonomic blockers scopolamine, atenolol, prazosin and hexamethonium. Implicit (Gaussian and t) and explicit (Clayton, Frank and Gumbel) copulas were calculated and compared to the conventional bivariate methods (Kendal, Pearson, Spearman and classical correlation). Further statistical validation was done using artificially generated surrogate data. A window sliding procedure for dynamic monitoring the signals' coupling strength is implemented. Results: Under the baseline physiological conditions, SBP-PI dependency is significant for time lags 0 s-4 s. Hexamethonium completely abolished the dependency, scopolamine abolished it for time lags 0 s-2 s, atenolol first slightly increased, than for lags greater than 2 s decreased the dependency and prazosin had no effect. Isospectral and isodistributional surrogate data tests confirm that copulas successfully notify the absence of dependency as well. Conclusion: Copula approach accurately captures the temporal fluctuations in dependency structures of SBP and PI, simultaneously enabling a visualization of dependency levels within the particular signal zones. An analysis showed that copulas are more sensitive than the conventional statistical measures, with Frank copula exhibiting the best characterization of SBP and PI dependency.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Biomedical Signal Processing & Control",
title = "Copula as a dynamic measure of cardiovascular signal interactions",
volume = "43",
pages = "250-264",
doi = "10.1016/j.bspc.2018.03.007"
}

Jovanović, S., Skorić, T., Sarenac, O., Milutinović-Smiljanić, S., Japundžić-Žigon, N.,& Bajić, D.. (2018). Copula as a dynamic measure of cardiovascular signal interactions. in Biomedical Signal Processing & Control
Elsevier Sci Ltd, Oxford., 43, 250-264.
https://doi.org/10.1016/j.bspc.2018.03.007

Jovanović S, Skorić T, Sarenac O, Milutinović-Smiljanić S, Japundžić-Žigon N, Bajić D. Copula as a dynamic measure of cardiovascular signal interactions. in Biomedical Signal Processing & Control. 2018;43:250-264.
doi:10.1016/j.bspc.2018.03.007 .

Jovanović, Slađana, Skorić, Tamara, Sarenac, Olivera, Milutinović-Smiljanić, Sanja, Japundžić-Žigon, Nina, Bajić, Dragana, "Copula as a dynamic measure of cardiovascular signal interactions" in Biomedical Signal Processing & Control, 43 (2018):250-264,
https://doi.org/10.1016/j.bspc.2018.03.007 . .

TY  - JOUR
AU  - Stanković, Marija
AU  - Pantić, Igor
AU  - de Luka, Silvio R.
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - Milutinović-Smiljanić, Sanja
AU  - Pantić, Senka
AU  - Trbovich, Alexander M.
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2129
AB  - The aim of the study was to examine alteration and possible application of fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely inflamed tissue. Acute inflammation was induced by injection of turpentine oil into the right and left hind limb muscles of mice, whereas control animals received intramuscular saline injection. After 12h, animals were anesthetised and treated muscles collected. The tissue was stained by hematoxylin and eosin, digital micrographs produced, enabling determination of fractal dimension of the cells, angular second moment and correlation of studied tissue. Histopathological analysis showed presence of inflammatory infiltrate and tissue damage in inflammatory group, whereas tissue structure in control group was preserved, devoid of inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of treated tissue in inflammatory group decreased in comparison to the control group. In this study, we were first to observe and report that fractal dimension of the cells, angular second moment, and correlation were reduced in acutely inflamed tissue, indicating loss of overall complexity of the cells in the tissue, the tissue uniformity and structure regularity. Fractal dimension, angular second moment and correlation could be useful methods for quantification of structural changes in acute inflammation. Lay Description The aim of this study was to examine alteration, and possible application of mathematical parameters fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely-inflamed tissue. An acute inflammation was induced by injection of turpentine oil into mice muscles, whereas control group received intramuscular injection of saline. After 12 h animals were anesthetized, and treated muscles were collected. The tissue was stained, and photos of the tissue were made. Mathematical parameters, namely fractal dimension, angular second moment, and correlation of the tissue photo, were determined by computer program. Standard histopathological analysis showed that inflammatory infiltrate and tissue damage were present in inflammatory group, whereas tissue structure in control group was preserved, and without inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of the treated tissue in inflammatory group decreased, when compared to control group. In this study we reported, for the first time, that fractal dimension of the cells, angular second moment, and correlation had decreased in acutely-inflamed tissue, indicating loss of overall complexity of cells in tissue, tissue uniformity, and structure regularity. Fractal dimension, angular second moment, and correlation could be useful methods for quantification of structural changes in acute inflammation.
PB  - Wiley, Hoboken
T2  - Journal of Microscopy
T1  - Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation
VL  - 261
IS  - 3
SP  - 277
EP  - 284
DO  - 10.1111/jmi.12330
ER  - 

@article{
author = "Stanković, Marija and Pantić, Igor and de Luka, Silvio R. and Puškaš, Nela and Zaletel, Ivan and Milutinović-Smiljanić, Sanja and Pantić, Senka and Trbovich, Alexander M.",
year = "2016",
abstract = "The aim of the study was to examine alteration and possible application of fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely inflamed tissue. Acute inflammation was induced by injection of turpentine oil into the right and left hind limb muscles of mice, whereas control animals received intramuscular saline injection. After 12h, animals were anesthetised and treated muscles collected. The tissue was stained by hematoxylin and eosin, digital micrographs produced, enabling determination of fractal dimension of the cells, angular second moment and correlation of studied tissue. Histopathological analysis showed presence of inflammatory infiltrate and tissue damage in inflammatory group, whereas tissue structure in control group was preserved, devoid of inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of treated tissue in inflammatory group decreased in comparison to the control group. In this study, we were first to observe and report that fractal dimension of the cells, angular second moment, and correlation were reduced in acutely inflamed tissue, indicating loss of overall complexity of the cells in the tissue, the tissue uniformity and structure regularity. Fractal dimension, angular second moment and correlation could be useful methods for quantification of structural changes in acute inflammation. Lay Description The aim of this study was to examine alteration, and possible application of mathematical parameters fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely-inflamed tissue. An acute inflammation was induced by injection of turpentine oil into mice muscles, whereas control group received intramuscular injection of saline. After 12 h animals were anesthetized, and treated muscles were collected. The tissue was stained, and photos of the tissue were made. Mathematical parameters, namely fractal dimension, angular second moment, and correlation of the tissue photo, were determined by computer program. Standard histopathological analysis showed that inflammatory infiltrate and tissue damage were present in inflammatory group, whereas tissue structure in control group was preserved, and without inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of the treated tissue in inflammatory group decreased, when compared to control group. In this study we reported, for the first time, that fractal dimension of the cells, angular second moment, and correlation had decreased in acutely-inflamed tissue, indicating loss of overall complexity of cells in tissue, tissue uniformity, and structure regularity. Fractal dimension, angular second moment, and correlation could be useful methods for quantification of structural changes in acute inflammation.",
publisher = "Wiley, Hoboken",
journal = "Journal of Microscopy",
title = "Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation",
volume = "261",
number = "3",
pages = "277-284",
doi = "10.1111/jmi.12330"
}

Stanković, M., Pantić, I., de Luka, S. R., Puškaš, N., Zaletel, I., Milutinović-Smiljanić, S., Pantić, S.,& Trbovich, A. M.. (2016). Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation. in Journal of Microscopy
Wiley, Hoboken., 261(3), 277-284.
https://doi.org/10.1111/jmi.12330

Stanković M, Pantić I, de Luka SR, Puškaš N, Zaletel I, Milutinović-Smiljanić S, Pantić S, Trbovich AM. Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation. in Journal of Microscopy. 2016;261(3):277-284.
doi:10.1111/jmi.12330 .

Stanković, Marija, Pantić, Igor, de Luka, Silvio R., Puškaš, Nela, Zaletel, Ivan, Milutinović-Smiljanić, Sanja, Pantić, Senka, Trbovich, Alexander M., "Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation" in Journal of Microscopy, 261, no. 3 (2016):277-284,
https://doi.org/10.1111/jmi.12330 . .

TY  - CONF
AU  - Stanković, Marija
AU  - Pantić, I
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - de Luka, Silvio R.
AU  - Milutinović-Smiljanić, Sanja
AU  - Trbovich, Alexander M.
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2006
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy
T1  - The role of IL-33/ST2 axis in enhancing inflammation, tissue damage and structural changes in acute inflammation
VL  - 70
SP  - 188
EP  - 188
UR  - https://hdl.handle.net/21.15107/rcub_smile_2006
ER  - 

@conference{
author = "Stanković, Marija and Pantić, I and Puškaš, Nela and Zaletel, Ivan and de Luka, Silvio R. and Milutinović-Smiljanić, Sanja and Trbovich, Alexander M.",
year = "2015",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "The role of IL-33/ST2 axis in enhancing inflammation, tissue damage and structural changes in acute inflammation",
volume = "70",
pages = "188-188",
url = "https://hdl.handle.net/21.15107/rcub_smile_2006"
}

Stanković, M., Pantić, I., Puškaš, N., Zaletel, I., de Luka, S. R., Milutinović-Smiljanić, S.,& Trbovich, A. M.. (2015). The role of IL-33/ST2 axis in enhancing inflammation, tissue damage and structural changes in acute inflammation. in Allergy
Wiley-Blackwell, Hoboken., 70, 188-188.
https://hdl.handle.net/21.15107/rcub_smile_2006

Stanković M, Pantić I, Puškaš N, Zaletel I, de Luka SR, Milutinović-Smiljanić S, Trbovich AM. The role of IL-33/ST2 axis in enhancing inflammation, tissue damage and structural changes in acute inflammation. in Allergy. 2015;70:188-188.
https://hdl.handle.net/21.15107/rcub_smile_2006 .

Stanković, Marija, Pantić, I, Puškaš, Nela, Zaletel, Ivan, de Luka, Silvio R., Milutinović-Smiljanić, Sanja, Trbovich, Alexander M., "The role of IL-33/ST2 axis in enhancing inflammation, tissue damage and structural changes in acute inflammation" in Allergy, 70 (2015):188-188,
https://hdl.handle.net/21.15107/rcub_smile_2006 .

TY  - JOUR
AU  - Stanković, Marija S.
AU  - Turuntas, Vladimir
AU  - de Luka, Silvio R.
AU  - Janković, Saša
AU  - Stefanović, Srđan
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - Milutinović-Smiljanić, Sanja
AU  - Trbovich, Alexander M.
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2003
AB  - Aim: The aim of this study was to examine the role of the IL-33/ST2 pathway in pathogenesis of acute inflammation by investigating its possible role in alteration of iron and hematological parameters in experimental model of acute inflammation. Material and methods: Wild-type and ST2 knockout BALB/c mice were divided into four groups: wild-type control group, ST2-/- control group, wild-type inflammatory group, and ST2-/- inflammatory group. Acute inflammation was induced by intramuscular injection of turpentine oil, while control groups were injected with saline. After 12 h animals were anesthetized, and the treated tissue, blood and spleen were collected. Iron concentration in the treated tissue, hemoglobin blood concentration, mean corpuscular hemoglobin (MCH), hematocrit, erythrocyte, neutrophil and lymphocyte blood count, and erythrocytes percentage in spleen were determined. Results: Iron concentration in the treated tissue was significantly higher in wild-type inflammatory group (WT-I) when compared to both, the wild-type control group (WT-C) and ST2-/- inflammatory group (KO-I). There was no significant difference in iron concentration between ST2-/- control group (KO-C) and the KO-I. MCH had significantly decreased in WT-I when compared to WT-C, while there was no significant difference between KO-C and KO-I. Hemoglobin blood concentration significantly increased in KO-I in comparison to KO-C, while it did not significantly differ between WT-I and KO-I. Erythrocyte count and hematocrit had significantly increased, while the percentage of erythrocytes in spleen decreased in both inflammatory groups when compared to their controls. Neutrophil count significantly decreased in WT-I, when compared to WT-C. Lymphocyte count decreased in both inflammatory groups when compared to their controls. Conclusion: Results of this study indicate that the IL-33/ST2 axis could have a role in the alteration of iron in acute inflammation, namely in an increase of iron concentration at the site of acute inflammation and a decrease of blood mean corpuscular hemoglobin.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Experimental & Molecular Pathology
T1  - Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation
VL  - 99
IS  - 3
SP  - 687
EP  - 692
DO  - 10.1016/j.yexmp.2015.11.016
ER  - 

@article{
author = "Stanković, Marija S. and Turuntas, Vladimir and de Luka, Silvio R. and Janković, Saša and Stefanović, Srđan and Puškaš, Nela and Zaletel, Ivan and Milutinović-Smiljanić, Sanja and Trbovich, Alexander M.",
year = "2015",
abstract = "Aim: The aim of this study was to examine the role of the IL-33/ST2 pathway in pathogenesis of acute inflammation by investigating its possible role in alteration of iron and hematological parameters in experimental model of acute inflammation. Material and methods: Wild-type and ST2 knockout BALB/c mice were divided into four groups: wild-type control group, ST2-/- control group, wild-type inflammatory group, and ST2-/- inflammatory group. Acute inflammation was induced by intramuscular injection of turpentine oil, while control groups were injected with saline. After 12 h animals were anesthetized, and the treated tissue, blood and spleen were collected. Iron concentration in the treated tissue, hemoglobin blood concentration, mean corpuscular hemoglobin (MCH), hematocrit, erythrocyte, neutrophil and lymphocyte blood count, and erythrocytes percentage in spleen were determined. Results: Iron concentration in the treated tissue was significantly higher in wild-type inflammatory group (WT-I) when compared to both, the wild-type control group (WT-C) and ST2-/- inflammatory group (KO-I). There was no significant difference in iron concentration between ST2-/- control group (KO-C) and the KO-I. MCH had significantly decreased in WT-I when compared to WT-C, while there was no significant difference between KO-C and KO-I. Hemoglobin blood concentration significantly increased in KO-I in comparison to KO-C, while it did not significantly differ between WT-I and KO-I. Erythrocyte count and hematocrit had significantly increased, while the percentage of erythrocytes in spleen decreased in both inflammatory groups when compared to their controls. Neutrophil count significantly decreased in WT-I, when compared to WT-C. Lymphocyte count decreased in both inflammatory groups when compared to their controls. Conclusion: Results of this study indicate that the IL-33/ST2 axis could have a role in the alteration of iron in acute inflammation, namely in an increase of iron concentration at the site of acute inflammation and a decrease of blood mean corpuscular hemoglobin.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Experimental & Molecular Pathology",
title = "Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation",
volume = "99",
number = "3",
pages = "687-692",
doi = "10.1016/j.yexmp.2015.11.016"
}

Stanković, M. S., Turuntas, V., de Luka, S. R., Janković, S., Stefanović, S., Puškaš, N., Zaletel, I., Milutinović-Smiljanić, S.,& Trbovich, A. M.. (2015). Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation. in Experimental & Molecular Pathology
Academic Press Inc Elsevier Science, San Diego., 99(3), 687-692.
https://doi.org/10.1016/j.yexmp.2015.11.016

Stanković MS, Turuntas V, de Luka SR, Janković S, Stefanović S, Puškaš N, Zaletel I, Milutinović-Smiljanić S, Trbovich AM. Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation. in Experimental & Molecular Pathology. 2015;99(3):687-692.
doi:10.1016/j.yexmp.2015.11.016 .

Stanković, Marija S., Turuntas, Vladimir, de Luka, Silvio R., Janković, Saša, Stefanović, Srđan, Puškaš, Nela, Zaletel, Ivan, Milutinović-Smiljanić, Sanja, Trbovich, Alexander M., "Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation" in Experimental & Molecular Pathology, 99, no. 3 (2015):687-692,
https://doi.org/10.1016/j.yexmp.2015.11.016 . .

TY  - JOUR
AU  - Milutinović-Smiljanić, Sanja
AU  - Sarenac, Olivera
AU  - Lozic-Đurić, Maja
AU  - Murphy, David
AU  - Japundžić-Žigon, Nina
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1834
AB  - Background and Purpose It is well recognized that vasopressin modulates the neurogenic control of the circulation. Here, we report the central mechanisms by which vasopressin modulates cardiovascular response to stress induced by immobilization. Experimental Approach Experiments were performed in conscious male Wistar rats equipped with radiotelemetric device for continuous measurement of haemodynamic parameters: systolic and diastolic BP and heart rate (HR). The functioning of the spontaneous baro-receptor reflex (BRR) was evaluated using the sequence method and the following parameters were evaluated: BRR sensitivity (BRS) and BRR effectiveness index (BEI). Key Results Under baseline physiological conditions intracerebroventricular injection of 100 and 500ng of selective non-peptide V1a or V1b or V2 receptor antagonist did not modify BP, HR and BRR. Rats exposed to 15min long stress by immobilization exhibited increase of BP, HR, reduction of BRS and no change in BEI. Pretreatment of rats with V1a receptor antagonist did not modulate the BP, HR, BRS and BEI response to stress. Pretreatment of rats with V1b receptor and V2 receptor antagonist, at both doses, prevented BRR desensitization and tachycardia, but failed to modulate stress-induced hypertension. Conclusions and Implications Vasopressin by the stimulation of central V1b- and V2-like receptors mediates stress-induced tachycardia and BRR desensitization. If these mechanisms are involved, BRR desensitization in heart failure and hypertension associated with poor outcome, they could be considered as novel targets for cardiovascular drug development.
PB  - Wiley-Blackwell, Hoboken
T2  - British Journal of Pharmacology
T1  - Evidence for involvement of central vasopressin V1b and V2 receptors in stress-induced baroreflex desensitization
VL  - 169
IS  - 4
SP  - 900
EP  - 908
DO  - 10.1111/bph.12161
ER  - 

@article{
author = "Milutinović-Smiljanić, Sanja and Sarenac, Olivera and Lozic-Đurić, Maja and Murphy, David and Japundžić-Žigon, Nina",
year = "2013",
abstract = "Background and Purpose It is well recognized that vasopressin modulates the neurogenic control of the circulation. Here, we report the central mechanisms by which vasopressin modulates cardiovascular response to stress induced by immobilization. Experimental Approach Experiments were performed in conscious male Wistar rats equipped with radiotelemetric device for continuous measurement of haemodynamic parameters: systolic and diastolic BP and heart rate (HR). The functioning of the spontaneous baro-receptor reflex (BRR) was evaluated using the sequence method and the following parameters were evaluated: BRR sensitivity (BRS) and BRR effectiveness index (BEI). Key Results Under baseline physiological conditions intracerebroventricular injection of 100 and 500ng of selective non-peptide V1a or V1b or V2 receptor antagonist did not modify BP, HR and BRR. Rats exposed to 15min long stress by immobilization exhibited increase of BP, HR, reduction of BRS and no change in BEI. Pretreatment of rats with V1a receptor antagonist did not modulate the BP, HR, BRS and BEI response to stress. Pretreatment of rats with V1b receptor and V2 receptor antagonist, at both doses, prevented BRR desensitization and tachycardia, but failed to modulate stress-induced hypertension. Conclusions and Implications Vasopressin by the stimulation of central V1b- and V2-like receptors mediates stress-induced tachycardia and BRR desensitization. If these mechanisms are involved, BRR desensitization in heart failure and hypertension associated with poor outcome, they could be considered as novel targets for cardiovascular drug development.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "British Journal of Pharmacology",
title = "Evidence for involvement of central vasopressin V1b and V2 receptors in stress-induced baroreflex desensitization",
volume = "169",
number = "4",
pages = "900-908",
doi = "10.1111/bph.12161"
}

Milutinović-Smiljanić, S., Sarenac, O., Lozic-Đurić, M., Murphy, D.,& Japundžić-Žigon, N.. (2013). Evidence for involvement of central vasopressin V1b and V2 receptors in stress-induced baroreflex desensitization. in British Journal of Pharmacology
Wiley-Blackwell, Hoboken., 169(4), 900-908.
https://doi.org/10.1111/bph.12161

Milutinović-Smiljanić S, Sarenac O, Lozic-Đurić M, Murphy D, Japundžić-Žigon N. Evidence for involvement of central vasopressin V1b and V2 receptors in stress-induced baroreflex desensitization. in British Journal of Pharmacology. 2013;169(4):900-908.
doi:10.1111/bph.12161 .

Milutinović-Smiljanić, Sanja, Sarenac, Olivera, Lozic-Đurić, Maja, Murphy, David, Japundžić-Žigon, Nina, "Evidence for involvement of central vasopressin V1b and V2 receptors in stress-induced baroreflex desensitization" in British Journal of Pharmacology, 169, no. 4 (2013):900-908,
https://doi.org/10.1111/bph.12161 . .

TY  - CONF
AU  - Loncar-Turukalo, Tatjana
AU  - Milutinović-Smiljanić, Sanja
AU  - Japundžić-Žigon, Nina
AU  - Bajić, Dragana
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1588
AB  - The baroreceptor reflex (BRR) bears the important part in short term blood pressure (BP) control. Joint order pattern analysis is proposed to assess the complex nature of BP and pulse interval (PI) dynamic. The BP and PI signals were acquired from conscious radiotelemetred Wistar male rats with intact BRR loop and pharmacologically opened BRR loop at different levels using blockade of beta-adrenergic, alpha-adrenergic and M-cholinergic receptors. The study revealed increase in complexity of relationship between BP and PI due to opening of BRR loop, as measured by permutation entropy and probability density function of transcriptions translating BP variations into PI responses. Synchronization measure significantly decreases in open BRR loop changing from 0.22 towards the values characteristic for random and independent data (0.02). It follows that BRR buffers random BP and PI changes and increases their synchronization.
PB  - IEEE, NEW YORK
C3  - Computing in Cardiology 2010, Vol 37
T1  - Joint Order Pattern Analysis to Assess Baroreflex Coupling of SBP and PI Series in Rats
VL  - 37
SP  - 249
EP  - 252
UR  - https://hdl.handle.net/21.15107/rcub_smile_1588
ER  - 

@conference{
author = "Loncar-Turukalo, Tatjana and Milutinović-Smiljanić, Sanja and Japundžić-Žigon, Nina and Bajić, Dragana",
year = "2010",
abstract = "The baroreceptor reflex (BRR) bears the important part in short term blood pressure (BP) control. Joint order pattern analysis is proposed to assess the complex nature of BP and pulse interval (PI) dynamic. The BP and PI signals were acquired from conscious radiotelemetred Wistar male rats with intact BRR loop and pharmacologically opened BRR loop at different levels using blockade of beta-adrenergic, alpha-adrenergic and M-cholinergic receptors. The study revealed increase in complexity of relationship between BP and PI due to opening of BRR loop, as measured by permutation entropy and probability density function of transcriptions translating BP variations into PI responses. Synchronization measure significantly decreases in open BRR loop changing from 0.22 towards the values characteristic for random and independent data (0.02). It follows that BRR buffers random BP and PI changes and increases their synchronization.",
publisher = "IEEE, NEW YORK",
journal = "Computing in Cardiology 2010, Vol 37",
title = "Joint Order Pattern Analysis to Assess Baroreflex Coupling of SBP and PI Series in Rats",
volume = "37",
pages = "249-252",
url = "https://hdl.handle.net/21.15107/rcub_smile_1588"
}

Loncar-Turukalo, T., Milutinović-Smiljanić, S., Japundžić-Žigon, N.,& Bajić, D.. (2010). Joint Order Pattern Analysis to Assess Baroreflex Coupling of SBP and PI Series in Rats. in Computing in Cardiology 2010, Vol 37
IEEE, NEW YORK., 37, 249-252.
https://hdl.handle.net/21.15107/rcub_smile_1588

Loncar-Turukalo T, Milutinović-Smiljanić S, Japundžić-Žigon N, Bajić D. Joint Order Pattern Analysis to Assess Baroreflex Coupling of SBP and PI Series in Rats. in Computing in Cardiology 2010, Vol 37. 2010;37:249-252.
https://hdl.handle.net/21.15107/rcub_smile_1588 .

Loncar-Turukalo, Tatjana, Milutinović-Smiljanić, Sanja, Japundžić-Žigon, Nina, Bajić, Dragana, "Joint Order Pattern Analysis to Assess Baroreflex Coupling of SBP and PI Series in Rats" in Computing in Cardiology 2010, Vol 37, 37 (2010):249-252,
https://hdl.handle.net/21.15107/rcub_smile_1588 .

TY  - JOUR
AU  - Stojičić, Sonja
AU  - Milutinović-Smiljanić, Sanja
AU  - Sarenac, Olivera
AU  - Milosavljević, S.
AU  - Paton, J. F. R.
AU  - Murphy, David
AU  - Japundžić-Žigon, Nina
PY  - 2008
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1431
AB  - To investigate the contribution of central vasopressin receptors to blood pressure (BP) and heart rate (HR) response to stress we injected non-peptide selective V-1a (SR49059), V-1b (SSR 149415), V-2 (SR 121463) receptor antagonists, diazepam or vehicle in the lateral cerebral ventricle of conscious freely moving rats stressed by blowing air on their heads for 2 min. Cardiovascular effects of stress were evaluated by analyzing maximum increase of BP and HR (MAX), latency of maximum response (LAT), integral under BP and HR curve (integral), duration of their recovery and spectral parameters of BP and HR indicative of increased sympathetic outflow (LFBP and LF/HFHR). Moreover, the increase of serum corticosterone was measured. Exposure to air-jet stress induced simultaneous increase in BP and HR followed by gradual decline during recovery while LFBP oscillation remained increased as well as serum corticosterone level. Rats pre-treated with vasopressin receptor antagonists were not sedated while diazepam induced sedation that persisted during exposure to stress. V-1a, V-1b and V-2, receptor antagonists applied separately did not modify basal values of cardiovascular parameters but prevented the increase in integral(BP). In addition, V-1b and V-2 receptor antagonists reduced BPMAX whereas V-1a, V-1b antagonist and diazepam reduced HRMAX induced by exposure to air-jet stress. All drugs shortened the recovery period, prevented the increase of LFBP without affecting the increase in serum corticosterone levels. Results indicate that vasopressin receptors located within the central nervous system mediate, in part, the cardiovascular response to air-jet stress without affecting either the neuroendocrine component or inducing sedation. They support the view that the V-1b receptor antagonist may be of potential therapeutic value in reducing arterial pressure induced by stress-related disorders.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Neuropharmacology
T1  - Blockade of central vasopressin receptors reduces the cardiovascular response to acute stress in freely moving rats
VL  - 54
IS  - 5
SP  - 824
EP  - 836
DO  - 10.1016/j.neuropharm.2007.12.013
ER  - 

@article{
author = "Stojičić, Sonja and Milutinović-Smiljanić, Sanja and Sarenac, Olivera and Milosavljević, S. and Paton, J. F. R. and Murphy, David and Japundžić-Žigon, Nina",
year = "2008",
abstract = "To investigate the contribution of central vasopressin receptors to blood pressure (BP) and heart rate (HR) response to stress we injected non-peptide selective V-1a (SR49059), V-1b (SSR 149415), V-2 (SR 121463) receptor antagonists, diazepam or vehicle in the lateral cerebral ventricle of conscious freely moving rats stressed by blowing air on their heads for 2 min. Cardiovascular effects of stress were evaluated by analyzing maximum increase of BP and HR (MAX), latency of maximum response (LAT), integral under BP and HR curve (integral), duration of their recovery and spectral parameters of BP and HR indicative of increased sympathetic outflow (LFBP and LF/HFHR). Moreover, the increase of serum corticosterone was measured. Exposure to air-jet stress induced simultaneous increase in BP and HR followed by gradual decline during recovery while LFBP oscillation remained increased as well as serum corticosterone level. Rats pre-treated with vasopressin receptor antagonists were not sedated while diazepam induced sedation that persisted during exposure to stress. V-1a, V-1b and V-2, receptor antagonists applied separately did not modify basal values of cardiovascular parameters but prevented the increase in integral(BP). In addition, V-1b and V-2 receptor antagonists reduced BPMAX whereas V-1a, V-1b antagonist and diazepam reduced HRMAX induced by exposure to air-jet stress. All drugs shortened the recovery period, prevented the increase of LFBP without affecting the increase in serum corticosterone levels. Results indicate that vasopressin receptors located within the central nervous system mediate, in part, the cardiovascular response to air-jet stress without affecting either the neuroendocrine component or inducing sedation. They support the view that the V-1b receptor antagonist may be of potential therapeutic value in reducing arterial pressure induced by stress-related disorders.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Neuropharmacology",
title = "Blockade of central vasopressin receptors reduces the cardiovascular response to acute stress in freely moving rats",
volume = "54",
number = "5",
pages = "824-836",
doi = "10.1016/j.neuropharm.2007.12.013"
}

Stojičić, S., Milutinović-Smiljanić, S., Sarenac, O., Milosavljević, S., Paton, J. F. R., Murphy, D.,& Japundžić-Žigon, N.. (2008). Blockade of central vasopressin receptors reduces the cardiovascular response to acute stress in freely moving rats. in Neuropharmacology
Pergamon-Elsevier Science Ltd, Oxford., 54(5), 824-836.
https://doi.org/10.1016/j.neuropharm.2007.12.013

Stojičić S, Milutinović-Smiljanić S, Sarenac O, Milosavljević S, Paton JFR, Murphy D, Japundžić-Žigon N. Blockade of central vasopressin receptors reduces the cardiovascular response to acute stress in freely moving rats. in Neuropharmacology. 2008;54(5):824-836.
doi:10.1016/j.neuropharm.2007.12.013 .

Stojičić, Sonja, Milutinović-Smiljanić, Sanja, Sarenac, Olivera, Milosavljević, S., Paton, J. F. R., Murphy, David, Japundžić-Žigon, Nina, "Blockade of central vasopressin receptors reduces the cardiovascular response to acute stress in freely moving rats" in Neuropharmacology, 54, no. 5 (2008):824-836,
https://doi.org/10.1016/j.neuropharm.2007.12.013 . .

TY  - JOUR
AU  - Stojičić, Sonja
AU  - Milutinović-Smiljanić, Sanja
AU  - Sarenac, Olivera
AU  - Živković, Slavoljub
AU  - Japundžić-Žigon, Nina
PY  - 2006
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1316
AB  - This study investigates the contribution of central vasopressin receptors in the modulation of systolic arterial pressure (SAP) and heart rate (HR) response to air-jet stress in conscious Wistar rats equipped with a femoral arterial catheter and intracerebroventricular cannula using novel non-peptide and selective vasopressin V, (SR49059) and V-1b (SSR149415) antagonists. The effects of stress on SAP and HR were evaluated by measuring the maximal response to stress, the latency of the maximal response, the duration of the recovery period, and the increase in the low frequency (LF) short-term variability component. Stress induced a parallel and almost immediate increase in both SAP and HR, followed by enhanced LF SAP variability in the recovery period. Pretreatment of rats with V-1a antagonist did not affect the maximal increase or the latency of SAP and HR response to acute stress, but shortened the recovery period of SAP and HR and prevented the increase in LF SAP. The V-1b antagonist reduced the maximal increase in SAP without affecting HR and their latencies, shortened the recovery period of SAP and inhibited the increase in LF SAP variability. These results indicate that both central V-1a and V-1b receptors mediate cardiovascular changes induced by air-jet stress in conscious rats.
PB  - Walter De Gruyter Gmbh, Berlin
T2  - Biomedizinische Technik
T1  - Central vasopressin V-1a and V-1b receptors modulate the cardiovascular response to air-jet stress in conscious rats
VL  - 51
IS  - 4
SP  - 268
EP  - 271
DO  - 10.1515/BMT.2006.053
ER  - 

@article{
author = "Stojičić, Sonja and Milutinović-Smiljanić, Sanja and Sarenac, Olivera and Živković, Slavoljub and Japundžić-Žigon, Nina",
year = "2006",
abstract = "This study investigates the contribution of central vasopressin receptors in the modulation of systolic arterial pressure (SAP) and heart rate (HR) response to air-jet stress in conscious Wistar rats equipped with a femoral arterial catheter and intracerebroventricular cannula using novel non-peptide and selective vasopressin V, (SR49059) and V-1b (SSR149415) antagonists. The effects of stress on SAP and HR were evaluated by measuring the maximal response to stress, the latency of the maximal response, the duration of the recovery period, and the increase in the low frequency (LF) short-term variability component. Stress induced a parallel and almost immediate increase in both SAP and HR, followed by enhanced LF SAP variability in the recovery period. Pretreatment of rats with V-1a antagonist did not affect the maximal increase or the latency of SAP and HR response to acute stress, but shortened the recovery period of SAP and HR and prevented the increase in LF SAP. The V-1b antagonist reduced the maximal increase in SAP without affecting HR and their latencies, shortened the recovery period of SAP and inhibited the increase in LF SAP variability. These results indicate that both central V-1a and V-1b receptors mediate cardiovascular changes induced by air-jet stress in conscious rats.",
publisher = "Walter De Gruyter Gmbh, Berlin",
journal = "Biomedizinische Technik",
title = "Central vasopressin V-1a and V-1b receptors modulate the cardiovascular response to air-jet stress in conscious rats",
volume = "51",
number = "4",
pages = "268-271",
doi = "10.1515/BMT.2006.053"
}

Stojičić, S., Milutinović-Smiljanić, S., Sarenac, O., Živković, S.,& Japundžić-Žigon, N.. (2006). Central vasopressin V-1a and V-1b receptors modulate the cardiovascular response to air-jet stress in conscious rats. in Biomedizinische Technik
Walter De Gruyter Gmbh, Berlin., 51(4), 268-271.
https://doi.org/10.1515/BMT.2006.053

Stojičić S, Milutinović-Smiljanić S, Sarenac O, Živković S, Japundžić-Žigon N. Central vasopressin V-1a and V-1b receptors modulate the cardiovascular response to air-jet stress in conscious rats. in Biomedizinische Technik. 2006;51(4):268-271.
doi:10.1515/BMT.2006.053 .

Stojičić, Sonja, Milutinović-Smiljanić, Sanja, Sarenac, Olivera, Živković, Slavoljub, Japundžić-Žigon, Nina, "Central vasopressin V-1a and V-1b receptors modulate the cardiovascular response to air-jet stress in conscious rats" in Biomedizinische Technik, 51, no. 4 (2006):268-271,
https://doi.org/10.1515/BMT.2006.053 . .

TY  - JOUR
AU  - Milutinović-Smiljanić, Sanja
AU  - Murphy, David
AU  - Japundžić-Žigon, Nina
PY  - 2006
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1319
AB  - Although it has been suggested that vasopressin (VP) acts within the central nervous system to modulate autonomic cardiovascular controls, the mechanisms involved are not understood. Using nonpeptide, selective V-1a, V-1b, and V-2 antagonists, in conscious rats, we assessed the roles of central VP receptors, under basal conditions, after the central application of exogenous VP, and after immobilization, on cardiovascular short-term variability. Equidistant sampling of blood pressure (BP) and heart rate (HR) at 20 Hz allowed direct spectral analysis in very-low frequency (VLF-BP), low-frequency (LF-BP), and high-frequency (HF-BP) blood pressure domains. The effect of VP antagonists and of exogenous VP on body temperature (T-b) was also investigated. Under basal conditions, V-1a antagonist increased HF-BP and T-b, and this was prevented by metamizol. V-1b antagonist enhanced HF-BP without affecting T-b, and V-2 antagonist increased VLF-BP variability which could be prevented by quinapril. Immobilization increased BP, LF-BP, HF-BP, and HF-HR variability. V-1a antagonist prevented BP and HR variability changes induced by immobilization and potentiated tachycardia. V-1b antagonist prevented BP but not HR variability changes, whereas V-2 antagonist had no effect. Exogenous VP increased systolic arterial pressure ( SAP) and HF-SAP variability, and this was prevented by V-1a and V-1b but not V-2 antagonist pretreatment. Our results suggest that, under basal conditions, VP, by stimulation of V-1a, V-1b, and cognate V-2 receptors, buffers BP variability, mostly due to thermoregulation. Immobilization and exogenous V-P, by stimulation of V-1a or V-1b, but not V-2 receptors, increases BP variability, revealing cardiorespiratory adjustment to stress and respiratory stimulation, respectively.
PB  - Amer Physiological Soc, Bethesda
T2  - American Journal of Physiology - Regulatory Integrative & Comparative Physiology
T1  - The role of central vasopressin receptors in the modulation of autonomic cardiovascular controls: a spectral analysis study
VL  - 291
IS  - 6
SP  - R1579
EP  - R1591
DO  - 10.1152/ajpregu.00764.2005
ER  - 

@article{
author = "Milutinović-Smiljanić, Sanja and Murphy, David and Japundžić-Žigon, Nina",
year = "2006",
abstract = "Although it has been suggested that vasopressin (VP) acts within the central nervous system to modulate autonomic cardiovascular controls, the mechanisms involved are not understood. Using nonpeptide, selective V-1a, V-1b, and V-2 antagonists, in conscious rats, we assessed the roles of central VP receptors, under basal conditions, after the central application of exogenous VP, and after immobilization, on cardiovascular short-term variability. Equidistant sampling of blood pressure (BP) and heart rate (HR) at 20 Hz allowed direct spectral analysis in very-low frequency (VLF-BP), low-frequency (LF-BP), and high-frequency (HF-BP) blood pressure domains. The effect of VP antagonists and of exogenous VP on body temperature (T-b) was also investigated. Under basal conditions, V-1a antagonist increased HF-BP and T-b, and this was prevented by metamizol. V-1b antagonist enhanced HF-BP without affecting T-b, and V-2 antagonist increased VLF-BP variability which could be prevented by quinapril. Immobilization increased BP, LF-BP, HF-BP, and HF-HR variability. V-1a antagonist prevented BP and HR variability changes induced by immobilization and potentiated tachycardia. V-1b antagonist prevented BP but not HR variability changes, whereas V-2 antagonist had no effect. Exogenous VP increased systolic arterial pressure ( SAP) and HF-SAP variability, and this was prevented by V-1a and V-1b but not V-2 antagonist pretreatment. Our results suggest that, under basal conditions, VP, by stimulation of V-1a, V-1b, and cognate V-2 receptors, buffers BP variability, mostly due to thermoregulation. Immobilization and exogenous V-P, by stimulation of V-1a or V-1b, but not V-2 receptors, increases BP variability, revealing cardiorespiratory adjustment to stress and respiratory stimulation, respectively.",
publisher = "Amer Physiological Soc, Bethesda",
journal = "American Journal of Physiology - Regulatory Integrative & Comparative Physiology",
title = "The role of central vasopressin receptors in the modulation of autonomic cardiovascular controls: a spectral analysis study",
volume = "291",
number = "6",
pages = "R1579-R1591",
doi = "10.1152/ajpregu.00764.2005"
}

Milutinović-Smiljanić, S., Murphy, D.,& Japundžić-Žigon, N.. (2006). The role of central vasopressin receptors in the modulation of autonomic cardiovascular controls: a spectral analysis study. in American Journal of Physiology - Regulatory Integrative & Comparative Physiology
Amer Physiological Soc, Bethesda., 291(6), R1579-R1591.
https://doi.org/10.1152/ajpregu.00764.2005

Milutinović-Smiljanić S, Murphy D, Japundžić-Žigon N. The role of central vasopressin receptors in the modulation of autonomic cardiovascular controls: a spectral analysis study. in American Journal of Physiology - Regulatory Integrative & Comparative Physiology. 2006;291(6):R1579-R1591.
doi:10.1152/ajpregu.00764.2005 .

Milutinović-Smiljanić, Sanja, Murphy, David, Japundžić-Žigon, Nina, "The role of central vasopressin receptors in the modulation of autonomic cardiovascular controls: a spectral analysis study" in American Journal of Physiology - Regulatory Integrative & Comparative Physiology, 291, no. 6 (2006):R1579-R1591,
https://doi.org/10.1152/ajpregu.00764.2005 . .

TY  - JOUR
AU  - Milutinović-Smiljanić, Sanja
AU  - Murphy, David
AU  - Japundžić-Žigon, Nina
PY  - 2006
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1307
AB  - The role of neurally born acetylcholine in the central modulation of cardiovascular short-term variability was assessed using a pharmacological probe physostigmine, a cholinesterase inhibitor that can act centrally also. Experiments were performed in instrumented conscious rats. Equidistant sampling at 20 Hz of systolic arterial pressure (SAP), diastolic arterial pressure (DAP) and heart rate (HR) allowed direct spectral analysis. Spectra were analysed in the whole, very-low frequency (VLF), low-frequency (LF) and high-frequency (HF) domains. Physostigmine, but not neostigmine, increased SAP, LF SAP and HF SAP variability while neostigmine, but not physostigmine, decreased HR without affecting HR variability. Atropine methyl nitrate prevented neostigmine-induced bradycardia and potentiated the effects of physostigmine on DAP, LF DAP and HF DAP variability. Atropine sulphate, hexamethonium, phentolamine and metoprolol inhibited physostigmine-induced increase of SAP and LF SAP. Pre-treatment of rats by quinapril prevented physostigmine-induced increase of SAP, but not of LF SAP, while the V-1a antagonist prevented the increase of HF SAP. The results suggest that central cholinergic neurons facilitate but do not create LF SAP and HF SAP variability. The effect of physostigmine on LF SAP seems to be mediated via central muscarinic sites and the peripheral sympathetic system, while non-muscarinic central sites and vasopressin pathways subserve the increase of HF SAP.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Neuropharmacology
T1  - Central cholinergic modulation of blood pressure short-term variability
VL  - 50
IS  - 7
SP  - 874
EP  - 883
DO  - 10.1016/j.neuropharm.2005.12.009
ER  - 

@article{
author = "Milutinović-Smiljanić, Sanja and Murphy, David and Japundžić-Žigon, Nina",
year = "2006",
abstract = "The role of neurally born acetylcholine in the central modulation of cardiovascular short-term variability was assessed using a pharmacological probe physostigmine, a cholinesterase inhibitor that can act centrally also. Experiments were performed in instrumented conscious rats. Equidistant sampling at 20 Hz of systolic arterial pressure (SAP), diastolic arterial pressure (DAP) and heart rate (HR) allowed direct spectral analysis. Spectra were analysed in the whole, very-low frequency (VLF), low-frequency (LF) and high-frequency (HF) domains. Physostigmine, but not neostigmine, increased SAP, LF SAP and HF SAP variability while neostigmine, but not physostigmine, decreased HR without affecting HR variability. Atropine methyl nitrate prevented neostigmine-induced bradycardia and potentiated the effects of physostigmine on DAP, LF DAP and HF DAP variability. Atropine sulphate, hexamethonium, phentolamine and metoprolol inhibited physostigmine-induced increase of SAP and LF SAP. Pre-treatment of rats by quinapril prevented physostigmine-induced increase of SAP, but not of LF SAP, while the V-1a antagonist prevented the increase of HF SAP. The results suggest that central cholinergic neurons facilitate but do not create LF SAP and HF SAP variability. The effect of physostigmine on LF SAP seems to be mediated via central muscarinic sites and the peripheral sympathetic system, while non-muscarinic central sites and vasopressin pathways subserve the increase of HF SAP.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Neuropharmacology",
title = "Central cholinergic modulation of blood pressure short-term variability",
volume = "50",
number = "7",
pages = "874-883",
doi = "10.1016/j.neuropharm.2005.12.009"
}

Milutinović-Smiljanić, S., Murphy, D.,& Japundžić-Žigon, N.. (2006). Central cholinergic modulation of blood pressure short-term variability. in Neuropharmacology
Pergamon-Elsevier Science Ltd, Oxford., 50(7), 874-883.
https://doi.org/10.1016/j.neuropharm.2005.12.009

Milutinović-Smiljanić S, Murphy D, Japundžić-Žigon N. Central cholinergic modulation of blood pressure short-term variability. in Neuropharmacology. 2006;50(7):874-883.
doi:10.1016/j.neuropharm.2005.12.009 .

Milutinović-Smiljanić, Sanja, Murphy, David, Japundžić-Žigon, Nina, "Central cholinergic modulation of blood pressure short-term variability" in Neuropharmacology, 50, no. 7 (2006):874-883,
https://doi.org/10.1016/j.neuropharm.2005.12.009 . .

TY  - JOUR
AU  - Arteaga, GM
AU  - Warren, CM
AU  - Milutinović-Smiljanić, Sanja
AU  - Martin, AF
AU  - Solaro, RJ
PY  - 2005
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1249
AB  - Alteration in myofilament response to Ca2+ is a major mechanism for depressed cardiac function after ischemia-reperfusion (I/R) dysfunction. We tested the hypothesis that hearts with increased myofilament response to Ca2+ are less susceptible to I/R. In one approach, we studied transgenic (TG) mice with a constitutive increase in myofilament Ca2+ sensitivity in which the adult form of cardiac troponin I (cTnI) is stoichiometrically replaced with the embryonic/neonatal isoform, slow skeletal TnI (ssTnI). We also studied mouse hearts with EMD-57033, which acts specifically to enhance myofilament response to Ca2+. We subjected isolated, perfused hearts to an I/R protocol consisting of 25 min of no-flow ischemia followed by 30 min of reperfusion. After I/R, developed pressure and rates of pressure change were significantly depressed and end-diastolic pressure was significantly elevated in nontransgenic (NTG) control hearts. These changes were significantly blunted in TG hearts and in NTG hearts perfused with EMD-57033 during reperfusion, with function returning to nearly baseline levels. Ca2+- and cross bridge-dependent activation, protein breakdown, and phosphorylation in detergent-extracted fiber bundles were also investigated. After I/R NTG fiber bundles exhibited a significant depression of cross bridge-dependent activation and Ca2+-activated tension and length dependence of activation that were not evident in TG preparations. Only NTG hearts demonstrated a significant increase in cTnI phosphorylation. Our results support the hypothesis that specific increases in myofilament Ca2+ sensitivity are able to diminish the effect of I/R on cardiac function.
PB  - Amer Physiological Soc, Bethesda
T2  - American Journal of Physiology - Heart & Circulatory Physiology
T1  - Specific enhancement of sarcomeric response to Ca2+ protects murine myocardium against ischemia-reperfusion dysfunction
VL  - 289
IS  - 5
SP  - H2183
EP  - H2192
DO  - 10.1152/ajpheart.00520.2005
ER  - 

@article{
author = "Arteaga, GM and Warren, CM and Milutinović-Smiljanić, Sanja and Martin, AF and Solaro, RJ",
year = "2005",
abstract = "Alteration in myofilament response to Ca2+ is a major mechanism for depressed cardiac function after ischemia-reperfusion (I/R) dysfunction. We tested the hypothesis that hearts with increased myofilament response to Ca2+ are less susceptible to I/R. In one approach, we studied transgenic (TG) mice with a constitutive increase in myofilament Ca2+ sensitivity in which the adult form of cardiac troponin I (cTnI) is stoichiometrically replaced with the embryonic/neonatal isoform, slow skeletal TnI (ssTnI). We also studied mouse hearts with EMD-57033, which acts specifically to enhance myofilament response to Ca2+. We subjected isolated, perfused hearts to an I/R protocol consisting of 25 min of no-flow ischemia followed by 30 min of reperfusion. After I/R, developed pressure and rates of pressure change were significantly depressed and end-diastolic pressure was significantly elevated in nontransgenic (NTG) control hearts. These changes were significantly blunted in TG hearts and in NTG hearts perfused with EMD-57033 during reperfusion, with function returning to nearly baseline levels. Ca2+- and cross bridge-dependent activation, protein breakdown, and phosphorylation in detergent-extracted fiber bundles were also investigated. After I/R NTG fiber bundles exhibited a significant depression of cross bridge-dependent activation and Ca2+-activated tension and length dependence of activation that were not evident in TG preparations. Only NTG hearts demonstrated a significant increase in cTnI phosphorylation. Our results support the hypothesis that specific increases in myofilament Ca2+ sensitivity are able to diminish the effect of I/R on cardiac function.",
publisher = "Amer Physiological Soc, Bethesda",
journal = "American Journal of Physiology - Heart & Circulatory Physiology",
title = "Specific enhancement of sarcomeric response to Ca2+ protects murine myocardium against ischemia-reperfusion dysfunction",
volume = "289",
number = "5",
pages = "H2183-H2192",
doi = "10.1152/ajpheart.00520.2005"
}

Arteaga, G., Warren, C., Milutinović-Smiljanić, S., Martin, A.,& Solaro, R.. (2005). Specific enhancement of sarcomeric response to Ca2+ protects murine myocardium against ischemia-reperfusion dysfunction. in American Journal of Physiology - Heart & Circulatory Physiology
Amer Physiological Soc, Bethesda., 289(5), H2183-H2192.
https://doi.org/10.1152/ajpheart.00520.2005

Arteaga G, Warren C, Milutinović-Smiljanić S, Martin A, Solaro R. Specific enhancement of sarcomeric response to Ca2+ protects murine myocardium against ischemia-reperfusion dysfunction. in American Journal of Physiology - Heart & Circulatory Physiology. 2005;289(5):H2183-H2192.
doi:10.1152/ajpheart.00520.2005 .

Arteaga, GM, Warren, CM, Milutinović-Smiljanić, Sanja, Martin, AF, Solaro, RJ, "Specific enhancement of sarcomeric response to Ca2+ protects murine myocardium against ischemia-reperfusion dysfunction" in American Journal of Physiology - Heart & Circulatory Physiology, 289, no. 5 (2005):H2183-H2192,
https://doi.org/10.1152/ajpheart.00520.2005 . .

TY  - JOUR
AU  - Japundžić-Žigon, Nina
AU  - Milutinović-Smiljanić, Sanja
AU  - Jovanović, A
PY  - 2004
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1220
AB  - Effects of V-1 (OPC-21268) and V-2 (OPC-31260) vasopressin antagonists on blood pressure (BP) short-term variability were investigated in adult spontaneously hypertensive rats (SHR) under basal conditions and after the stimulation of vasopressin release by hemorrhage. BP was recorded intra-arterially and sampled at 20 Hz to be analyzed on a personal computer. BP time spectra were calculated on 30 stationary overlapping 2048 point-time series. Spectral power was estimated in total (0.00976 - 3 Hz), very low frequency (VLF: 0.00976 - 0.195 Hz), low frequency (LF: 0.195 - 0.605 Hz), and high frequency (HF: 0. 8 - 3 Hz) regions. Under basal conditions a V, antagonist (5 mg/kg, i.v.) decreased BP without affecting BP variability, while combined (V-1 + V-2) blockade or V-2 blockade (1 mg/kg, i.v.) alone did not affect cardiovascular parameters. Mild hemorrhage (5 ml/kg per min) increased HF-BP variability, while moderate (10 ml/kg per min) and massive (15 ml/kg per min) hemorrhage did not affect it. In V-1, but not V-2, antagonist pre-treated SHR HF-BP increased significantly after moderate and massive hemorrhage. V-1 or V-2 antagonist pre-treatment also enhanced VLF-BP variability during massive hemorrhage. Moreover V-1 blockade prevented hemorrhage-induced bradycardia, while V-2 blockade potentiated it. It follows that in adult SHR, vasopressin buffers BP oscillations in HF and VLF frequency domains only in hypovolaemic conditions and that the modulation of the autonomic adjustment of the HR to hemorrhage by vasopressin is preserved.
PB  - Japanese Pharmacological Soc, Kyoto
T2  - Journal of Pharmacological Sciences
T1  - Effects of nonpeptide and selective V-1 and V-2 antagonists on blood pressure short-term variability in spontaneously hypertensive rats
VL  - 95
IS  - 1
SP  - 47
EP  - 55
DO  - 10.1254/jphs.95.47
ER  - 

@article{
author = "Japundžić-Žigon, Nina and Milutinović-Smiljanić, Sanja and Jovanović, A",
year = "2004",
abstract = "Effects of V-1 (OPC-21268) and V-2 (OPC-31260) vasopressin antagonists on blood pressure (BP) short-term variability were investigated in adult spontaneously hypertensive rats (SHR) under basal conditions and after the stimulation of vasopressin release by hemorrhage. BP was recorded intra-arterially and sampled at 20 Hz to be analyzed on a personal computer. BP time spectra were calculated on 30 stationary overlapping 2048 point-time series. Spectral power was estimated in total (0.00976 - 3 Hz), very low frequency (VLF: 0.00976 - 0.195 Hz), low frequency (LF: 0.195 - 0.605 Hz), and high frequency (HF: 0. 8 - 3 Hz) regions. Under basal conditions a V, antagonist (5 mg/kg, i.v.) decreased BP without affecting BP variability, while combined (V-1 + V-2) blockade or V-2 blockade (1 mg/kg, i.v.) alone did not affect cardiovascular parameters. Mild hemorrhage (5 ml/kg per min) increased HF-BP variability, while moderate (10 ml/kg per min) and massive (15 ml/kg per min) hemorrhage did not affect it. In V-1, but not V-2, antagonist pre-treated SHR HF-BP increased significantly after moderate and massive hemorrhage. V-1 or V-2 antagonist pre-treatment also enhanced VLF-BP variability during massive hemorrhage. Moreover V-1 blockade prevented hemorrhage-induced bradycardia, while V-2 blockade potentiated it. It follows that in adult SHR, vasopressin buffers BP oscillations in HF and VLF frequency domains only in hypovolaemic conditions and that the modulation of the autonomic adjustment of the HR to hemorrhage by vasopressin is preserved.",
publisher = "Japanese Pharmacological Soc, Kyoto",
journal = "Journal of Pharmacological Sciences",
title = "Effects of nonpeptide and selective V-1 and V-2 antagonists on blood pressure short-term variability in spontaneously hypertensive rats",
volume = "95",
number = "1",
pages = "47-55",
doi = "10.1254/jphs.95.47"
}

Japundžić-Žigon, N., Milutinović-Smiljanić, S.,& Jovanović, A.. (2004). Effects of nonpeptide and selective V-1 and V-2 antagonists on blood pressure short-term variability in spontaneously hypertensive rats. in Journal of Pharmacological Sciences
Japanese Pharmacological Soc, Kyoto., 95(1), 47-55.
https://doi.org/10.1254/jphs.95.47

Japundžić-Žigon N, Milutinović-Smiljanić S, Jovanović A. Effects of nonpeptide and selective V-1 and V-2 antagonists on blood pressure short-term variability in spontaneously hypertensive rats. in Journal of Pharmacological Sciences. 2004;95(1):47-55.
doi:10.1254/jphs.95.47 .

Japundžić-Žigon, Nina, Milutinović-Smiljanić, Sanja, Jovanović, A, "Effects of nonpeptide and selective V-1 and V-2 antagonists on blood pressure short-term variability in spontaneously hypertensive rats" in Journal of Pharmacological Sciences, 95, no. 1 (2004):47-55,
https://doi.org/10.1254/jphs.95.47 . .