TY  - JOUR
AU  - Pejin, Boris
AU  - Ćirić, Ana
AU  - Marković, Dejan
AU  - Tommonaro, Giuseppina
AU  - Soković, Marina
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2131
AB  - This work extends in vitro screening of antimicrobial activity of avarol, the marine natural product firstly isolated from the Mediterranean sponge Dysidea avara. Its anticandidial activity was evaluated by microdilution method against eight Candida strains, two ATCC and six clinical ones. At a different extent this compound was proven to be active against all the strains tested (MIC 0.8-6.0g/mL and MFC 1.6-12.0g/mL, respectively). According to the best of our knowledge, this is the first report on avarol activity towards any yeast strain which may be of relevance for Alzheimer's disease. Indeed, avarol derivatives showing moderate AChE activity should be screened for anticandidial activity both in vitro and in vivo. [GRAPHICS] .
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - In vitro avarol does affect the growth of Candida sp.
VL  - 30
IS  - 17
SP  - 1956
EP  - 1960
DO  - 10.1080/14786419.2015.1091454
ER  - 

@article{
author = "Pejin, Boris and Ćirić, Ana and Marković, Dejan and Tommonaro, Giuseppina and Soković, Marina",
year = "2016",
abstract = "This work extends in vitro screening of antimicrobial activity of avarol, the marine natural product firstly isolated from the Mediterranean sponge Dysidea avara. Its anticandidial activity was evaluated by microdilution method against eight Candida strains, two ATCC and six clinical ones. At a different extent this compound was proven to be active against all the strains tested (MIC 0.8-6.0g/mL and MFC 1.6-12.0g/mL, respectively). According to the best of our knowledge, this is the first report on avarol activity towards any yeast strain which may be of relevance for Alzheimer's disease. Indeed, avarol derivatives showing moderate AChE activity should be screened for anticandidial activity both in vitro and in vivo. [GRAPHICS] .",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "In vitro avarol does affect the growth of Candida sp.",
volume = "30",
number = "17",
pages = "1956-1960",
doi = "10.1080/14786419.2015.1091454"
}

Pejin, B., Ćirić, A., Marković, D., Tommonaro, G.,& Soković, M.. (2016). In vitro avarol does affect the growth of Candida sp.. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 30(17), 1956-1960.
https://doi.org/10.1080/14786419.2015.1091454

Pejin B, Ćirić A, Marković D, Tommonaro G, Soković M. In vitro avarol does affect the growth of Candida sp.. in Natural Product Research. 2016;30(17):1956-1960.
doi:10.1080/14786419.2015.1091454 .

Pejin, Boris, Ćirić, Ana, Marković, Dejan, Tommonaro, Giuseppina, Soković, Marina, "In vitro avarol does affect the growth of Candida sp." in Natural Product Research, 30, no. 17 (2016):1956-1960,
https://doi.org/10.1080/14786419.2015.1091454 . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Marković, Tatjana
AU  - Mojović, Miloš
AU  - Pejin, Boris
AU  - Savić, Aleksandar
AU  - Perić, Tamara
AU  - Marković, Dejan
AU  - Stević, Tatjana
AU  - Soković, Marina
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1829
AB  - The aim of this study was to examine chemical composition and biological activity of Gaultheria procumbens L essential oil (EO) against food spoilage and oral microorganisms. The components of EO were identified by GC-MS. Antimicrobial activity was determined against food spoilage (five bacteria and six fungal species) and oral microorganisms (eight bacteria and thirty two fungal species) by microdilution and microplate biofilm assay, antioxidant activity was tested using the persistent free radical 2,2-diphenyl-1-picryl hydrazyl (DPPH), while antiradical activity was examined by fluorescence spectroscopy and electron paramagnetic resonance spectroscopy (EPR). GC-MS analysis showed that methyl salicylate (96.90%) was the main component of the oil. Essential oil inhibited the growth of all microorganisms tested, i.e. food and oral bacteria and fungi, respectively (MIC 0.18-3.00 mg/ml and MBC 1.25-4.00 mg/ml; MIC 0.73-5.00 mg/ml and MFC 2.92-26.67 mg/ml); The oil effectively inhibited the biofilm formation of oral Streptococcus mutans and Candida albicans as well (MIC 25.00 MBC 50.00 mg/ml; MIC 12.50, MFC 50.00 mg/ml). In addition, oil exhibited a dose-dependent DPPH-radical-scavenging activity with IC50 value of 30.61 mg/ml. The specific fluorescence probe 2-[6-(4'-amino)phenoxy-3H-xanten-3-on-9-yl] benzoic acid (APP) and the the spin trap 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO), capable for simultaneous detection of different free radical specie were used in antiradical activity of the oil measurements. Oil showed a moderate antiradical activity, reducing quantity of produced hydroxyl radicals to about 20% of initial value. This study succeeds in creating directly comparable and quantitative data for the oil unsufficiently examined so far.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops & Products
T1  - Chemical composition and biological activity of Gaultheria procumbens L. essential oil
VL  - 49
SP  - 561
EP  - 567
DO  - 10.1016/j.indcrop.2013.06.002
ER  - 

@article{
author = "Nikolić, Miloš and Marković, Tatjana and Mojović, Miloš and Pejin, Boris and Savić, Aleksandar and Perić, Tamara and Marković, Dejan and Stević, Tatjana and Soković, Marina",
year = "2013",
abstract = "The aim of this study was to examine chemical composition and biological activity of Gaultheria procumbens L essential oil (EO) against food spoilage and oral microorganisms. The components of EO were identified by GC-MS. Antimicrobial activity was determined against food spoilage (five bacteria and six fungal species) and oral microorganisms (eight bacteria and thirty two fungal species) by microdilution and microplate biofilm assay, antioxidant activity was tested using the persistent free radical 2,2-diphenyl-1-picryl hydrazyl (DPPH), while antiradical activity was examined by fluorescence spectroscopy and electron paramagnetic resonance spectroscopy (EPR). GC-MS analysis showed that methyl salicylate (96.90%) was the main component of the oil. Essential oil inhibited the growth of all microorganisms tested, i.e. food and oral bacteria and fungi, respectively (MIC 0.18-3.00 mg/ml and MBC 1.25-4.00 mg/ml; MIC 0.73-5.00 mg/ml and MFC 2.92-26.67 mg/ml); The oil effectively inhibited the biofilm formation of oral Streptococcus mutans and Candida albicans as well (MIC 25.00 MBC 50.00 mg/ml; MIC 12.50, MFC 50.00 mg/ml). In addition, oil exhibited a dose-dependent DPPH-radical-scavenging activity with IC50 value of 30.61 mg/ml. The specific fluorescence probe 2-[6-(4'-amino)phenoxy-3H-xanten-3-on-9-yl] benzoic acid (APP) and the the spin trap 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO), capable for simultaneous detection of different free radical specie were used in antiradical activity of the oil measurements. Oil showed a moderate antiradical activity, reducing quantity of produced hydroxyl radicals to about 20% of initial value. This study succeeds in creating directly comparable and quantitative data for the oil unsufficiently examined so far.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops & Products",
title = "Chemical composition and biological activity of Gaultheria procumbens L. essential oil",
volume = "49",
pages = "561-567",
doi = "10.1016/j.indcrop.2013.06.002"
}

Nikolić, M., Marković, T., Mojović, M., Pejin, B., Savić, A., Perić, T., Marković, D., Stević, T.,& Soković, M.. (2013). Chemical composition and biological activity of Gaultheria procumbens L. essential oil. in Industrial Crops & Products
Elsevier, Amsterdam., 49, 561-567.
https://doi.org/10.1016/j.indcrop.2013.06.002

Nikolić M, Marković T, Mojović M, Pejin B, Savić A, Perić T, Marković D, Stević T, Soković M. Chemical composition and biological activity of Gaultheria procumbens L. essential oil. in Industrial Crops & Products. 2013;49:561-567.
doi:10.1016/j.indcrop.2013.06.002 .

Nikolić, Miloš, Marković, Tatjana, Mojović, Miloš, Pejin, Boris, Savić, Aleksandar, Perić, Tamara, Marković, Dejan, Stević, Tatjana, Soković, Marina, "Chemical composition and biological activity of Gaultheria procumbens L. essential oil" in Industrial Crops & Products, 49 (2013):561-567,
https://doi.org/10.1016/j.indcrop.2013.06.002 . .