TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Andrić, Miroslav
AU  - Knežević, Aleksandra
AU  - Soldatović, Ivan
AU  - Nikolić, Nadja
AU  - Karalić, Danijela
AU  - Milašin, Jelena
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1996
AB  - Introduction: Different genotypes of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) possess specific pathogenic abilities because of various interactions with the host's immune system and differences in cell tropism. The aim of this study was to determine the distribution of HCMV and EBV genotypes in apical periodontitis lesions in relation to their clinical and histopathologic features. Methods: One hundred samples of apical periodontitis lesions and 25 control samples (healthy pulp tissue) were collected. The presence of HCMV glycoprotein B (gB) and EBV nuclear antigen-2 genotypes was analyzed by nested polymerase chain reaction and restriction fragment length polymorphisms analysis. Results: EBV and HCMV were detected in apical periodontitis lesions at significantly higher frequencies than in healthy pulp controls (P = .020 and P = .020, respectively). HCMV gB type II was significantly more frequent compared with gB type I in the examined groups (P = .036). No HCMV gB type III or IV products were found. In both periapical lesions and controls, EBV-1 occurred more often compared with EBV-2 (P = .001). Dual EBV and HCMV coinfection was more frequently detected in large-size periapical lesions (P = .038). Conclusions: Both HCMV and EBV are associated with inflammatory processes of periapical bone destruction. HCMV gB type II and EBV-1 are the most prevalent genotypes in apical periodontitis lesions.
PB  - Elsevier Science Inc, New York
T2  - Journal of Endodontics
T1  - Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions
VL  - 41
IS  - 11
SP  - 1847
EP  - 1851
DO  - 10.1016/j.joen.2015.08.027
ER  - 

@article{
author = "Jakovljević, Aleksandar and Andrić, Miroslav and Knežević, Aleksandra and Soldatović, Ivan and Nikolić, Nadja and Karalić, Danijela and Milašin, Jelena",
year = "2015",
abstract = "Introduction: Different genotypes of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) possess specific pathogenic abilities because of various interactions with the host's immune system and differences in cell tropism. The aim of this study was to determine the distribution of HCMV and EBV genotypes in apical periodontitis lesions in relation to their clinical and histopathologic features. Methods: One hundred samples of apical periodontitis lesions and 25 control samples (healthy pulp tissue) were collected. The presence of HCMV glycoprotein B (gB) and EBV nuclear antigen-2 genotypes was analyzed by nested polymerase chain reaction and restriction fragment length polymorphisms analysis. Results: EBV and HCMV were detected in apical periodontitis lesions at significantly higher frequencies than in healthy pulp controls (P = .020 and P = .020, respectively). HCMV gB type II was significantly more frequent compared with gB type I in the examined groups (P = .036). No HCMV gB type III or IV products were found. In both periapical lesions and controls, EBV-1 occurred more often compared with EBV-2 (P = .001). Dual EBV and HCMV coinfection was more frequently detected in large-size periapical lesions (P = .038). Conclusions: Both HCMV and EBV are associated with inflammatory processes of periapical bone destruction. HCMV gB type II and EBV-1 are the most prevalent genotypes in apical periodontitis lesions.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Endodontics",
title = "Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions",
volume = "41",
number = "11",
pages = "1847-1851",
doi = "10.1016/j.joen.2015.08.027"
}

Jakovljević, A., Andrić, M., Knežević, A., Soldatović, I., Nikolić, N., Karalić, D.,& Milašin, J.. (2015). Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions. in Journal of Endodontics
Elsevier Science Inc, New York., 41(11), 1847-1851.
https://doi.org/10.1016/j.joen.2015.08.027

Jakovljević A, Andrić M, Knežević A, Soldatović I, Nikolić N, Karalić D, Milašin J. Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions. in Journal of Endodontics. 2015;41(11):1847-1851.
doi:10.1016/j.joen.2015.08.027 .

Jakovljević, Aleksandar, Andrić, Miroslav, Knežević, Aleksandra, Soldatović, Ivan, Nikolić, Nadja, Karalić, Danijela, Milašin, Jelena, "Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions" in Journal of Endodontics, 41, no. 11 (2015):1847-1851,
https://doi.org/10.1016/j.joen.2015.08.027 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Knežević, Aleksandra
AU  - Karalić, Danijela
AU  - Soldatović, Ivan
AU  - Popović, Branka
AU  - Milašin, Jelena
AU  - Andrić, Miroslav
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1986
AB  - This study aimed to compare the levels of tumour necrosis factor-alpha (TNF-), interleukin-1 beta (IL-1) and interleukin-6 (IL-6) between apical periodontitis lesions with different clinical and histological features. Based on clinical data and history of disease, 100 human apical periodontitis lesions were categorised as either asymptomatic or symptomatic lesions. According to histological examination, lesions were divided into periapical granulomas and radicular cysts. Pulp tissues of 25 impacted wisdom teeth were used as controls. Homogenised tissue samples were centrifuged and supernatants were used for the determination of cytokine levels by enzyme-linked immunosorbent assay. Significantly higher levels of IL-1 and IL-6 were found in symptomatic lesions compared with asymptomatic lesions and control tissues (P lt 0.001, P lt 0.001, respectively). The concentration of IL-1 was significantly higher in radicular cysts compared with periapical granulomas (P=0.003). Symptomatic lesions, as judged by high local production of IL-1 and IL-6, represent an immunologically active stage of the disease.
PB  - Wiley, Hoboken
T2  - Australian Endodontic Journal
T1  - Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings
VL  - 41
IS  - 2
SP  - 72
EP  - 77
DO  - 10.1111/aej.12072
ER  - 

@article{
author = "Jakovljević, Aleksandar and Knežević, Aleksandra and Karalić, Danijela and Soldatović, Ivan and Popović, Branka and Milašin, Jelena and Andrić, Miroslav",
year = "2015",
abstract = "This study aimed to compare the levels of tumour necrosis factor-alpha (TNF-), interleukin-1 beta (IL-1) and interleukin-6 (IL-6) between apical periodontitis lesions with different clinical and histological features. Based on clinical data and history of disease, 100 human apical periodontitis lesions were categorised as either asymptomatic or symptomatic lesions. According to histological examination, lesions were divided into periapical granulomas and radicular cysts. Pulp tissues of 25 impacted wisdom teeth were used as controls. Homogenised tissue samples were centrifuged and supernatants were used for the determination of cytokine levels by enzyme-linked immunosorbent assay. Significantly higher levels of IL-1 and IL-6 were found in symptomatic lesions compared with asymptomatic lesions and control tissues (P lt 0.001, P lt 0.001, respectively). The concentration of IL-1 was significantly higher in radicular cysts compared with periapical granulomas (P=0.003). Symptomatic lesions, as judged by high local production of IL-1 and IL-6, represent an immunologically active stage of the disease.",
publisher = "Wiley, Hoboken",
journal = "Australian Endodontic Journal",
title = "Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings",
volume = "41",
number = "2",
pages = "72-77",
doi = "10.1111/aej.12072"
}

Jakovljević, A., Knežević, A., Karalić, D., Soldatović, I., Popović, B., Milašin, J.,& Andrić, M.. (2015). Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings. in Australian Endodontic Journal
Wiley, Hoboken., 41(2), 72-77.
https://doi.org/10.1111/aej.12072

Jakovljević A, Knežević A, Karalić D, Soldatović I, Popović B, Milašin J, Andrić M. Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings. in Australian Endodontic Journal. 2015;41(2):72-77.
doi:10.1111/aej.12072 .

Jakovljević, Aleksandar, Knežević, Aleksandra, Karalić, Danijela, Soldatović, Ivan, Popović, Branka, Milašin, Jelena, Andrić, Miroslav, "Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings" in Australian Endodontic Journal, 41, no. 2 (2015):72-77,
https://doi.org/10.1111/aej.12072 . .