TY  - JOUR
AU  - Hadži-Mihailović, Miloš
AU  - Petrović, Renata
AU  - Raybaud, Helene
AU  - Stanimirović, Dragan
AU  - Koray, Meltem Ozar
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2208
AB  - Purpose: Oral lichen planus (OLP) is an autoimmune skin and mucosal disorder. The range of malignant transformation in OLP varies between 0.1-3%. p53 is a tumor suppressor protein. Defective p53 could allow abnormal cells to proliferate, resulting in cancer. p53 plays an important role in cell cycle control and apoptosis and loss of p53 function has been demonstrated in about half of all human cancers. The purpose of the study was to investigate the malignant potential of OLP on the basis of p53 expression and to correlate p53 expression with clinical and histopathological features of OLP. Methods: 40 patients with OLP underwent biopsy. All tissue samples were treated immunohistochemicaly using avidin-biotin peroxidase complex method. Results: In 80% of OLP specimens the nuclei of basal and parabasal keratinocytes were p53-positive, but in low numbers. Low percentage of p53-positive cells in older and medium percentage of p53-positive cells in younger group of OLP patients were noted. Higher intensity of p53 stained keratinocytes, no matter their low number, could represent mutant and more stable form of p53 protein, and at the same time signal for monitoring of disease due to potential malignant transformation. Low percentage and weak intensity of p53-positive cells was detected mostly in OLP specimens with highly expressed civatte bodies (CB). Upregulation of apoptosis didn't correspond with the expression of CB. Conclusion: We believe that low percentage of p53-positive and well-marked keratinocytes in OLP represent the influence of mutant p53 protein, and that increasing expression of this protein could serve as a valuable diagnostic sign of early carcinogenesis. According to our results intensity of p53 coloration of keratinocytes could help assessing the malignant potential of OLP.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Expression and role of p53 in oral lichen planus patients
VL  - 22
IS  - 5
SP  - 1278
EP  - 1286
UR  - https://hdl.handle.net/21.15107/rcub_smile_2208
ER  - 

@article{
author = "Hadži-Mihailović, Miloš and Petrović, Renata and Raybaud, Helene and Stanimirović, Dragan and Koray, Meltem Ozar",
year = "2017",
abstract = "Purpose: Oral lichen planus (OLP) is an autoimmune skin and mucosal disorder. The range of malignant transformation in OLP varies between 0.1-3%. p53 is a tumor suppressor protein. Defective p53 could allow abnormal cells to proliferate, resulting in cancer. p53 plays an important role in cell cycle control and apoptosis and loss of p53 function has been demonstrated in about half of all human cancers. The purpose of the study was to investigate the malignant potential of OLP on the basis of p53 expression and to correlate p53 expression with clinical and histopathological features of OLP. Methods: 40 patients with OLP underwent biopsy. All tissue samples were treated immunohistochemicaly using avidin-biotin peroxidase complex method. Results: In 80% of OLP specimens the nuclei of basal and parabasal keratinocytes were p53-positive, but in low numbers. Low percentage of p53-positive cells in older and medium percentage of p53-positive cells in younger group of OLP patients were noted. Higher intensity of p53 stained keratinocytes, no matter their low number, could represent mutant and more stable form of p53 protein, and at the same time signal for monitoring of disease due to potential malignant transformation. Low percentage and weak intensity of p53-positive cells was detected mostly in OLP specimens with highly expressed civatte bodies (CB). Upregulation of apoptosis didn't correspond with the expression of CB. Conclusion: We believe that low percentage of p53-positive and well-marked keratinocytes in OLP represent the influence of mutant p53 protein, and that increasing expression of this protein could serve as a valuable diagnostic sign of early carcinogenesis. According to our results intensity of p53 coloration of keratinocytes could help assessing the malignant potential of OLP.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Expression and role of p53 in oral lichen planus patients",
volume = "22",
number = "5",
pages = "1278-1286",
url = "https://hdl.handle.net/21.15107/rcub_smile_2208"
}

Hadži-Mihailović, M., Petrović, R., Raybaud, H., Stanimirović, D.,& Koray, M. O.. (2017). Expression and role of p53 in oral lichen planus patients. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 22(5), 1278-1286.
https://hdl.handle.net/21.15107/rcub_smile_2208

Hadži-Mihailović M, Petrović R, Raybaud H, Stanimirović D, Koray MO. Expression and role of p53 in oral lichen planus patients. in Journal of BUON. 2017;22(5):1278-1286.
https://hdl.handle.net/21.15107/rcub_smile_2208 .

Hadži-Mihailović, Miloš, Petrović, Renata, Raybaud, Helene, Stanimirović, Dragan, Koray, Meltem Ozar, "Expression and role of p53 in oral lichen planus patients" in Journal of BUON, 22, no. 5 (2017):1278-1286,
https://hdl.handle.net/21.15107/rcub_smile_2208 .

TY  - JOUR
AU  - Hadži-Mihailović, Miloš
AU  - Čakić, Saša
AU  - Janković, Saša
AU  - Raybaud, Helene
AU  - Nedeljković, Nenad
AU  - Janković, Ljiljana
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1748
AB  - Purpose: The monoclonal antibody Ki-67 detects a nuclear antigen that is present only in proliferating cells. This is of particular interest for the analysis of the proliferation rates of malignant tumors. The aim of this study was to investigate the malignant potential of oral lichen planus (OLP) on the basis of expression of Ki-67 in healthy individuals (HI), patients with OLP and patients with squamous cell carcinoma (SCC), and to see for any potential interdependence between Ki-67 expression and different clinical and histopathological parameters in OLP. Methods: Immunohistochemistry for Ki-67 was carried out using an avidin-biotin peroxidase complex method. Results: Ki-67 was more expressed in keratinocytes and lymphocytes of OLP patients compared with HI, but less compared with patients with SCC. Keratinocytes and lymphocytes stained with Ki-67 in OLP patients were significantly higher in males, and in OLP specimens showed less developed civatte bodies (CB) and thickening of the basal membrane (TBM). Conclusion: Ki-67 may not serve as prognostic biomarker in oral cancer development from the initially diagnosed OLP, but it could help selecting patients with higher need of follow up for prevention of malignancy.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Ki-67 expression in oral lichen planus
VL  - 17
IS  - 1
SP  - 132
EP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_smile_1748
ER  - 

@article{
author = "Hadži-Mihailović, Miloš and Čakić, Saša and Janković, Saša and Raybaud, Helene and Nedeljković, Nenad and Janković, Ljiljana",
year = "2012",
abstract = "Purpose: The monoclonal antibody Ki-67 detects a nuclear antigen that is present only in proliferating cells. This is of particular interest for the analysis of the proliferation rates of malignant tumors. The aim of this study was to investigate the malignant potential of oral lichen planus (OLP) on the basis of expression of Ki-67 in healthy individuals (HI), patients with OLP and patients with squamous cell carcinoma (SCC), and to see for any potential interdependence between Ki-67 expression and different clinical and histopathological parameters in OLP. Methods: Immunohistochemistry for Ki-67 was carried out using an avidin-biotin peroxidase complex method. Results: Ki-67 was more expressed in keratinocytes and lymphocytes of OLP patients compared with HI, but less compared with patients with SCC. Keratinocytes and lymphocytes stained with Ki-67 in OLP patients were significantly higher in males, and in OLP specimens showed less developed civatte bodies (CB) and thickening of the basal membrane (TBM). Conclusion: Ki-67 may not serve as prognostic biomarker in oral cancer development from the initially diagnosed OLP, but it could help selecting patients with higher need of follow up for prevention of malignancy.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Ki-67 expression in oral lichen planus",
volume = "17",
number = "1",
pages = "132-137",
url = "https://hdl.handle.net/21.15107/rcub_smile_1748"
}

Hadži-Mihailović, M., Čakić, S., Janković, S., Raybaud, H., Nedeljković, N.,& Janković, L.. (2012). Ki-67 expression in oral lichen planus. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 17(1), 132-137.
https://hdl.handle.net/21.15107/rcub_smile_1748

Hadži-Mihailović M, Čakić S, Janković S, Raybaud H, Nedeljković N, Janković L. Ki-67 expression in oral lichen planus. in Journal of BUON. 2012;17(1):132-137.
https://hdl.handle.net/21.15107/rcub_smile_1748 .

Hadži-Mihailović, Miloš, Čakić, Saša, Janković, Saša, Raybaud, Helene, Nedeljković, Nenad, Janković, Ljiljana, "Ki-67 expression in oral lichen planus" in Journal of BUON, 17, no. 1 (2012):132-137,
https://hdl.handle.net/21.15107/rcub_smile_1748 .

TY  - JOUR
AU  - Hadži-Mihailović, Miloš
AU  - Raybaud, Helene
AU  - Montei, R.
AU  - Čakić, Saša
AU  - Đurić, M.
AU  - Janković, Ljiljana
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1591
AB  - Purpose: The aim of this study was to investigate the malignant potential of oral lichen planus (OLP) on the basis of the expression of the Bcl-2 marker in healthy individuals (H), patients with OLP and patients with squamous cell carcinoma (SCC), and to establish potential interdependence between expression of Bcl-2 and the different clinical and histopathological parameters in H, OLP, and SCC. Methods: Immunohistochemistry for Bcl-2 was carried out using an avidin-biotin peroxidase complex method. Tissue sections were treated with mouse monoclonal antibody against Bcl-2 (124, DAKO A/S, Denmark; dilution 1/100). Immunohistochemical parameters measured included total tissue area, total stained area and intensity of stain. Results: Keratinocytes were not Bcl-2 positive in H and were sparsely positive in OLP. The number of lymphocytes stained with Bcl-2 was significantly lower in Has compared to patients with SCC and OLP. Bcl-2 staining was weak to moderate in OLP, and moderate to intense in SCC. Bcl-2-positive lymphocytes were more expressed in older OLP patients (>55 years), and in OLP specimens with orthokeratinized epithelium, less developed acanthosis and highest grade of lymphocyte expression. Conclusion: Bcl-2 may not serve as a prognostic biomarker in oral cancer development from OLP, but it could help in selecting patients with higher need of follow up to prevent malignancy.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Bcl-2 expression and its possible influence on malignant transformation of oral lichen planus
VL  - 15
IS  - 2
SP  - 362
EP  - 368
UR  - https://hdl.handle.net/21.15107/rcub_smile_1591
ER  - 

@article{
author = "Hadži-Mihailović, Miloš and Raybaud, Helene and Montei, R. and Čakić, Saša and Đurić, M. and Janković, Ljiljana",
year = "2010",
abstract = "Purpose: The aim of this study was to investigate the malignant potential of oral lichen planus (OLP) on the basis of the expression of the Bcl-2 marker in healthy individuals (H), patients with OLP and patients with squamous cell carcinoma (SCC), and to establish potential interdependence between expression of Bcl-2 and the different clinical and histopathological parameters in H, OLP, and SCC. Methods: Immunohistochemistry for Bcl-2 was carried out using an avidin-biotin peroxidase complex method. Tissue sections were treated with mouse monoclonal antibody against Bcl-2 (124, DAKO A/S, Denmark; dilution 1/100). Immunohistochemical parameters measured included total tissue area, total stained area and intensity of stain. Results: Keratinocytes were not Bcl-2 positive in H and were sparsely positive in OLP. The number of lymphocytes stained with Bcl-2 was significantly lower in Has compared to patients with SCC and OLP. Bcl-2 staining was weak to moderate in OLP, and moderate to intense in SCC. Bcl-2-positive lymphocytes were more expressed in older OLP patients (>55 years), and in OLP specimens with orthokeratinized epithelium, less developed acanthosis and highest grade of lymphocyte expression. Conclusion: Bcl-2 may not serve as a prognostic biomarker in oral cancer development from OLP, but it could help in selecting patients with higher need of follow up to prevent malignancy.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Bcl-2 expression and its possible influence on malignant transformation of oral lichen planus",
volume = "15",
number = "2",
pages = "362-368",
url = "https://hdl.handle.net/21.15107/rcub_smile_1591"
}

Hadži-Mihailović, M., Raybaud, H., Montei, R., Čakić, S., Đurić, M.,& Janković, L.. (2010). Bcl-2 expression and its possible influence on malignant transformation of oral lichen planus. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 15(2), 362-368.
https://hdl.handle.net/21.15107/rcub_smile_1591

Hadži-Mihailović M, Raybaud H, Montei R, Čakić S, Đurić M, Janković L. Bcl-2 expression and its possible influence on malignant transformation of oral lichen planus. in Journal of BUON. 2010;15(2):362-368.
https://hdl.handle.net/21.15107/rcub_smile_1591 .

Hadži-Mihailović, Miloš, Raybaud, Helene, Montei, R., Čakić, Saša, Đurić, M., Janković, Ljiljana, "Bcl-2 expression and its possible influence on malignant transformation of oral lichen planus" in Journal of BUON, 15, no. 2 (2010):362-368,
https://hdl.handle.net/21.15107/rcub_smile_1591 .

TY  - JOUR
AU  - Hadži-Mihailović, Miloš
AU  - Raybaud, Helene
AU  - Monteil, R.
AU  - Janković, Ljiljana
PY  - 2009
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1491
AB  - Purpose: To investigate the malignant potential of oral lichen planus (OLP) on the basis of expression of the Fas/FasL markers in healthy individuals (H), OLP patients and patients with squamous cell carcinoma (SCC). Patients and methods: 40 patients with OLP and two control groups were included in this research (H and patients with SCC). Immunohislochemistry for Fas and FasL was carried out using an avidin-biotin peroxidase complex method. Results: Only a low percentage of infiltrating lymphocytes and no keratinocytes were Fas-positive in OLP specimens. The highest percentage of Fas-staining keratinocytes in our survey was identified mostly in H and patients with well-differentiated SCC In most cases of SCC, OLP and H a high percentage of keratinocytes and lymphocytes were FasL-positive. FasL expression was negatively correlated with the degree of cell differentiation and apoptosis. Taking into consideration that all carcinomas in this survey were highly differentiated, it is not surprising that no statistically significant differences in FasL expression between H, OLP and SCC specimens were detected Conclusion: Downregulation of Fas expression in keratinocytes and lymphocytes of OLP specimens, together with upregulation of FasL, may serve as initial prognostic biomarker in oral cancer development.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Expression of Fas/FasL in patients with oral lichen planus
VL  - 14
IS  - 3
SP  - 487
EP  - 493
UR  - https://hdl.handle.net/21.15107/rcub_smile_1491
ER  - 

@article{
author = "Hadži-Mihailović, Miloš and Raybaud, Helene and Monteil, R. and Janković, Ljiljana",
year = "2009",
abstract = "Purpose: To investigate the malignant potential of oral lichen planus (OLP) on the basis of expression of the Fas/FasL markers in healthy individuals (H), OLP patients and patients with squamous cell carcinoma (SCC). Patients and methods: 40 patients with OLP and two control groups were included in this research (H and patients with SCC). Immunohislochemistry for Fas and FasL was carried out using an avidin-biotin peroxidase complex method. Results: Only a low percentage of infiltrating lymphocytes and no keratinocytes were Fas-positive in OLP specimens. The highest percentage of Fas-staining keratinocytes in our survey was identified mostly in H and patients with well-differentiated SCC In most cases of SCC, OLP and H a high percentage of keratinocytes and lymphocytes were FasL-positive. FasL expression was negatively correlated with the degree of cell differentiation and apoptosis. Taking into consideration that all carcinomas in this survey were highly differentiated, it is not surprising that no statistically significant differences in FasL expression between H, OLP and SCC specimens were detected Conclusion: Downregulation of Fas expression in keratinocytes and lymphocytes of OLP specimens, together with upregulation of FasL, may serve as initial prognostic biomarker in oral cancer development.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Expression of Fas/FasL in patients with oral lichen planus",
volume = "14",
number = "3",
pages = "487-493",
url = "https://hdl.handle.net/21.15107/rcub_smile_1491"
}

Hadži-Mihailović, M., Raybaud, H., Monteil, R.,& Janković, L.. (2009). Expression of Fas/FasL in patients with oral lichen planus. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 14(3), 487-493.
https://hdl.handle.net/21.15107/rcub_smile_1491

Hadži-Mihailović M, Raybaud H, Monteil R, Janković L. Expression of Fas/FasL in patients with oral lichen planus. in Journal of BUON. 2009;14(3):487-493.
https://hdl.handle.net/21.15107/rcub_smile_1491 .

Hadži-Mihailović, Miloš, Raybaud, Helene, Monteil, R., Janković, Ljiljana, "Expression of Fas/FasL in patients with oral lichen planus" in Journal of BUON, 14, no. 3 (2009):487-493,
https://hdl.handle.net/21.15107/rcub_smile_1491 .