TY  - JOUR
AU  - Matić Petrović, Sanja
AU  - Nikolić, Nadja
AU  - Toljić, Boško
AU  - Arambašić-Jovanović, Jelena
AU  - Miličić, Biljana
AU  - Miličić, Tanja
AU  - Jotić, Aleksandra
AU  - Vidaković, Melita
AU  - Milašin, Jelena
AU  - Pucar, Ana
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2571
AB  - Objectives: Aiming to show that periodontitis (PD) and type 2 diabetes (T2D) are bidirectionally related and potentially linked by inflammatory cytokines, we searched for association between -308 G/A Tumor necrosis factor-alpha (TNFα), +252A/G Lymphotoxin-alpha (LTα), +36A/G Tumor necrosis factor receptor 1 (TNFR1) and +676 T/G tumor necrosis factor receptor 2 (TNFR2) single nucleotide polymorphisms (SNPs) and: risk of PD or PD + T2D; periodontitis parameters in PD and PD + T2D; serum levels of cytokines/their receptors. Relationship between periodontal inflammation and serum cytokine/receptor levels was also assessed.

Design: Subjects were stratified as: 57 healthy controls (HC); 58 PD; 65 PD + T2D. Sociodemographic, environmental, behavioral and periodontal clinical data were recorded. SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism, while cytokines/receptors levels were quantified by enzyme-linked immunosorbent assay. Impact of periodontal inflammation was measured using periodontal inflamed surface area (PISA).

Results: TNFα AA genotype showed protective effect in T2D + PD compared to PD, even adjusted for behavioral/environmental factors (OR 0.18; 95 %CI 0.037-0.886; p = 0.035). LTα AG heterozygotes had increased risk of PD (OR 3.27; 95 %CI 1.35-7.96; p = 0.016), while TNFR2 TG genotype had protective effect (OR = 0.44; 95 %CI 0.954-0.9794; p = 0.043). TNFR1 AA was predictor of periodontal pocket depth and clinical attachment loss in PD. Correlation between TNFR2 concentration and PISA was negative in PD, positive in PD + T2D.

Conclusions: None of the SNPs showed cross-susceptibility between PD and T2D. + 252A/G LTα and +676 T/G TNFR2 SNPs are associated with PD risk. Periodontal destruction in healthy individuals is influenced by TNFR1 genotype. Impact of periodontal on systemic inflammation is masked by T2D.
PB  - Elsevier Inc.
T2  - Archives of Oral Biology
T1  - The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population
VL  - 120
SP  - 104929
DO  - 10.1016/j.archoralbio.2020.104929
ER  - 

@article{
author = "Matić Petrović, Sanja and Nikolić, Nadja and Toljić, Boško and Arambašić-Jovanović, Jelena and Miličić, Biljana and Miličić, Tanja and Jotić, Aleksandra and Vidaković, Melita and Milašin, Jelena and Pucar, Ana",
year = "2020",
abstract = "Objectives: Aiming to show that periodontitis (PD) and type 2 diabetes (T2D) are bidirectionally related and potentially linked by inflammatory cytokines, we searched for association between -308 G/A Tumor necrosis factor-alpha (TNFα), +252A/G Lymphotoxin-alpha (LTα), +36A/G Tumor necrosis factor receptor 1 (TNFR1) and +676 T/G tumor necrosis factor receptor 2 (TNFR2) single nucleotide polymorphisms (SNPs) and: risk of PD or PD + T2D; periodontitis parameters in PD and PD + T2D; serum levels of cytokines/their receptors. Relationship between periodontal inflammation and serum cytokine/receptor levels was also assessed.

Design: Subjects were stratified as: 57 healthy controls (HC); 58 PD; 65 PD + T2D. Sociodemographic, environmental, behavioral and periodontal clinical data were recorded. SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism, while cytokines/receptors levels were quantified by enzyme-linked immunosorbent assay. Impact of periodontal inflammation was measured using periodontal inflamed surface area (PISA).

Results: TNFα AA genotype showed protective effect in T2D + PD compared to PD, even adjusted for behavioral/environmental factors (OR 0.18; 95 %CI 0.037-0.886; p = 0.035). LTα AG heterozygotes had increased risk of PD (OR 3.27; 95 %CI 1.35-7.96; p = 0.016), while TNFR2 TG genotype had protective effect (OR = 0.44; 95 %CI 0.954-0.9794; p = 0.043). TNFR1 AA was predictor of periodontal pocket depth and clinical attachment loss in PD. Correlation between TNFR2 concentration and PISA was negative in PD, positive in PD + T2D.

Conclusions: None of the SNPs showed cross-susceptibility between PD and T2D. + 252A/G LTα and +676 T/G TNFR2 SNPs are associated with PD risk. Periodontal destruction in healthy individuals is influenced by TNFR1 genotype. Impact of periodontal on systemic inflammation is masked by T2D.",
publisher = "Elsevier Inc.",
journal = "Archives of Oral Biology",
title = "The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population",
volume = "120",
pages = "104929",
doi = "10.1016/j.archoralbio.2020.104929"
}

Matić Petrović, S., Nikolić, N., Toljić, B., Arambašić-Jovanović, J., Miličić, B., Miličić, T., Jotić, A., Vidaković, M., Milašin, J.,& Pucar, A.. (2020). The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population. in Archives of Oral Biology
Elsevier Inc.., 120, 104929.
https://doi.org/10.1016/j.archoralbio.2020.104929

Matić Petrović S, Nikolić N, Toljić B, Arambašić-Jovanović J, Miličić B, Miličić T, Jotić A, Vidaković M, Milašin J, Pucar A. The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population. in Archives of Oral Biology. 2020;120:104929.
doi:10.1016/j.archoralbio.2020.104929 .

Matić Petrović, Sanja, Nikolić, Nadja, Toljić, Boško, Arambašić-Jovanović, Jelena, Miličić, Biljana, Miličić, Tanja, Jotić, Aleksandra, Vidaković, Melita, Milašin, Jelena, Pucar, Ana, "The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population" in Archives of Oral Biology, 120 (2020):104929,
https://doi.org/10.1016/j.archoralbio.2020.104929 . .

TY  - JOUR
AU  - Petrović, Milan
AU  - Jelovac, Drago
AU  - Antić, Svetlana
AU  - Antunović, Marija
AU  - Lukić, Nikola
AU  - Sabani, Melvil
AU  - Mudrak, Joerg
AU  - Jezdić, Zoran
AU  - Pucar, Ana
AU  - Stefanović, Aleksandar
AU  - Kuzmanović, Čedomir
AU  - Nikolić, Danilo
AU  - Konstantinović, Vitomir
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2443
AB  - This retrospective cohort study aims to describe characteristics of patients with MRONJ, to identify factors associated with MRONJ development, and to examine variables associated with favourable outcome. Totally 32 patients were followed and observed: 21 females and 11 males, in the age range 35-84 in the period from 2009 to 2018. Clinical, radiological examination (Orthopantomograph and CBCT) and biopsy were performed in order to achieve diagnosis. Demographic and clinical variables were taken into consideration: sex, age, primary disease, medication type, mode of delivery, anatomic location, drug treatment duration, timing of tooth extraction, chemotherapy, presence of bone metastasis, aetiology of MRONJ, disease stage, and treatment modality. MRONJ developed under osteoporosis and malignant disease in 11 and 21 patients, respectively. MRONJ development was triggered by tooth extraction or trauma in 30 out of 32 cases, whereas the two patients developed MRONJ spontaneously. Stages I, II, and III were confirmed in 5 (16%), 18 (58%), and 9 (28%) patients, respectively. Mandible was affected in 23 (72%) patients. MRONJ was treated in our department by conservative and surgical modality. In this study we found that 65% of all patients were classified in the cured/improvement group and 35% in the stable/progression group. The female gender, osteoporosis as primary disease, oral regime intake, shorter period on BPs, earlier stage of disease, and specific anatomic localisation (frontal and premolar maxilla) were factors associated with better response to therapy and favourable clinical outcome. Comprehensive treatment protocol and further randomized studies are necessary for further improvements.
PB  - Hindawi Ltd, London
T2  - Biomed Research International
T1  - Medication-Related Osteonecrosis of the Jaws: Two Center Retrospective Cohort Studies
VL  - 2019
DO  - 10.1155/2019/8345309
ER  - 

@article{
author = "Petrović, Milan and Jelovac, Drago and Antić, Svetlana and Antunović, Marija and Lukić, Nikola and Sabani, Melvil and Mudrak, Joerg and Jezdić, Zoran and Pucar, Ana and Stefanović, Aleksandar and Kuzmanović, Čedomir and Nikolić, Danilo and Konstantinović, Vitomir",
year = "2019",
abstract = "This retrospective cohort study aims to describe characteristics of patients with MRONJ, to identify factors associated with MRONJ development, and to examine variables associated with favourable outcome. Totally 32 patients were followed and observed: 21 females and 11 males, in the age range 35-84 in the period from 2009 to 2018. Clinical, radiological examination (Orthopantomograph and CBCT) and biopsy were performed in order to achieve diagnosis. Demographic and clinical variables were taken into consideration: sex, age, primary disease, medication type, mode of delivery, anatomic location, drug treatment duration, timing of tooth extraction, chemotherapy, presence of bone metastasis, aetiology of MRONJ, disease stage, and treatment modality. MRONJ developed under osteoporosis and malignant disease in 11 and 21 patients, respectively. MRONJ development was triggered by tooth extraction or trauma in 30 out of 32 cases, whereas the two patients developed MRONJ spontaneously. Stages I, II, and III were confirmed in 5 (16%), 18 (58%), and 9 (28%) patients, respectively. Mandible was affected in 23 (72%) patients. MRONJ was treated in our department by conservative and surgical modality. In this study we found that 65% of all patients were classified in the cured/improvement group and 35% in the stable/progression group. The female gender, osteoporosis as primary disease, oral regime intake, shorter period on BPs, earlier stage of disease, and specific anatomic localisation (frontal and premolar maxilla) were factors associated with better response to therapy and favourable clinical outcome. Comprehensive treatment protocol and further randomized studies are necessary for further improvements.",
publisher = "Hindawi Ltd, London",
journal = "Biomed Research International",
title = "Medication-Related Osteonecrosis of the Jaws: Two Center Retrospective Cohort Studies",
volume = "2019",
doi = "10.1155/2019/8345309"
}

Petrović, M., Jelovac, D., Antić, S., Antunović, M., Lukić, N., Sabani, M., Mudrak, J., Jezdić, Z., Pucar, A., Stefanović, A., Kuzmanović, Č., Nikolić, D.,& Konstantinović, V.. (2019). Medication-Related Osteonecrosis of the Jaws: Two Center Retrospective Cohort Studies. in Biomed Research International
Hindawi Ltd, London., 2019.
https://doi.org/10.1155/2019/8345309

Petrović M, Jelovac D, Antić S, Antunović M, Lukić N, Sabani M, Mudrak J, Jezdić Z, Pucar A, Stefanović A, Kuzmanović Č, Nikolić D, Konstantinović V. Medication-Related Osteonecrosis of the Jaws: Two Center Retrospective Cohort Studies. in Biomed Research International. 2019;2019.
doi:10.1155/2019/8345309 .

Petrović, Milan, Jelovac, Drago, Antić, Svetlana, Antunović, Marija, Lukić, Nikola, Sabani, Melvil, Mudrak, Joerg, Jezdić, Zoran, Pucar, Ana, Stefanović, Aleksandar, Kuzmanović, Čedomir, Nikolić, Danilo, Konstantinović, Vitomir, "Medication-Related Osteonecrosis of the Jaws: Two Center Retrospective Cohort Studies" in Biomed Research International, 2019 (2019),
https://doi.org/10.1155/2019/8345309 . .

TY  - JOUR
AU  - Matić-Petrović, Sanja
AU  - Radunović, Milena
AU  - Barać, Milena
AU  - Kuzmanović-Pfićer, Jovana
AU  - Pavlica, Dušan
AU  - Arsić-Arsenijević, Valentina
AU  - Pucar, Ana
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2463
AB  - Objectives The aim of this cross-sectional observational study was to compare the prevalence of different oral Candida spp. in patients with Type 2 Diabetes and chronic periodontitis in two oral sites: dorsal surface of the tongue and subgingival area. In order to determine subgingival areas as potential reservoirs of yeasts, this study aimed to find differences in the yeasts' detection between the dorsum of the tongue, as the oral site most commonly inhabited with microorganisms, and subgingival samples. Additionally, potential predictors for the yeasts prevalence were determined. Material and methods Subjects (N = 146) were divided into four groups: group A-healthy individuals without periodontitis, group B-healthy individuals with chronic periodontitis, group C-Type 2 Diabetes patients with good glycoregulation and Chronic periodontitis and group D-Type 2 Diabetes patients with poor glycoregulation and Chronic periodontitis. Samples were obtained from the tongue by swabbing. Subgingival plaque samples were taken by paper points and periodontal curette. Isolation and identification of different Candida spp. was done using ChromAgar medium. In addition, germ-tube production and carbohydrate assimilation tests were performed. Results The prevalence of Candida spp. was higher in diabetics with poor glycoregulation. The most frequently isolated species was Candida albicans followed by Candida glabrata and Candida tropicalis. In 15.6% of cases, Candida spp. was present in the subgingival area while absent on the tongue. Multivariate regression model showed that HbA1c was Candida spp. predictor for both locations. Conclusions Our results confirmed that there are Candida spp. carriers among subjects with clinically healthy oral mucosa. Also, this study identified subgingival areas as potential reservoirs of these pathogenic species. Glycoregulation has been recognized as a positive predictor factor of Candida spp.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects
VL  - 14
IS  - 1
DO  - 10.1371/journal.pone.0210527
ER  - 

@article{
author = "Matić-Petrović, Sanja and Radunović, Milena and Barać, Milena and Kuzmanović-Pfićer, Jovana and Pavlica, Dušan and Arsić-Arsenijević, Valentina and Pucar, Ana",
year = "2019",
abstract = "Objectives The aim of this cross-sectional observational study was to compare the prevalence of different oral Candida spp. in patients with Type 2 Diabetes and chronic periodontitis in two oral sites: dorsal surface of the tongue and subgingival area. In order to determine subgingival areas as potential reservoirs of yeasts, this study aimed to find differences in the yeasts' detection between the dorsum of the tongue, as the oral site most commonly inhabited with microorganisms, and subgingival samples. Additionally, potential predictors for the yeasts prevalence were determined. Material and methods Subjects (N = 146) were divided into four groups: group A-healthy individuals without periodontitis, group B-healthy individuals with chronic periodontitis, group C-Type 2 Diabetes patients with good glycoregulation and Chronic periodontitis and group D-Type 2 Diabetes patients with poor glycoregulation and Chronic periodontitis. Samples were obtained from the tongue by swabbing. Subgingival plaque samples were taken by paper points and periodontal curette. Isolation and identification of different Candida spp. was done using ChromAgar medium. In addition, germ-tube production and carbohydrate assimilation tests were performed. Results The prevalence of Candida spp. was higher in diabetics with poor glycoregulation. The most frequently isolated species was Candida albicans followed by Candida glabrata and Candida tropicalis. In 15.6% of cases, Candida spp. was present in the subgingival area while absent on the tongue. Multivariate regression model showed that HbA1c was Candida spp. predictor for both locations. Conclusions Our results confirmed that there are Candida spp. carriers among subjects with clinically healthy oral mucosa. Also, this study identified subgingival areas as potential reservoirs of these pathogenic species. Glycoregulation has been recognized as a positive predictor factor of Candida spp.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects",
volume = "14",
number = "1",
doi = "10.1371/journal.pone.0210527"
}

Matić-Petrović, S., Radunović, M., Barać, M., Kuzmanović-Pfićer, J., Pavlica, D., Arsić-Arsenijević, V.,& Pucar, A.. (2019). Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects. in PLoS One
Public Library Science, San Francisco., 14(1).
https://doi.org/10.1371/journal.pone.0210527

Matić-Petrović S, Radunović M, Barać M, Kuzmanović-Pfićer J, Pavlica D, Arsić-Arsenijević V, Pucar A. Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects. in PLoS One. 2019;14(1).
doi:10.1371/journal.pone.0210527 .

Matić-Petrović, Sanja, Radunović, Milena, Barać, Milena, Kuzmanović-Pfićer, Jovana, Pavlica, Dušan, Arsić-Arsenijević, Valentina, Pucar, Ana, "Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects" in PLoS One, 14, no. 1 (2019),
https://doi.org/10.1371/journal.pone.0210527 . .

TY  - JOUR
AU  - Matić-Petrović, Sanja
AU  - Đorđević, Mihajlo
AU  - Radunović, Milena
AU  - Živanović, Tanja
AU  - Pavlica, Dušan
AU  - Pucar, Ana
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2406
AB  - Background/Aim: Geographic tongue (GT), a benign self-limiting condition (inflammation) is commonly seen in practice. Although quite easy for clinical diagnosis, in clinical practice it is commonly misdiagnosed and treated as Candida infection. The main aim of this study was to compare the prevalence of Candida spp. on tongue in patients with GT and subjects with clinically healthy oral mucosa. Additional aims were to evaluate subjective symptoms, uncontrolled usage of prescribed or non-prescribed antifungal topical medications and the presence of the cancerophobia or other health concerns in patients with GT. Material and Methods: A total of 70 subjects were divided into two groups: Group B - patients with diagnosed Geographic tongue and group A - aged and gender matched controls with clinically health oral mucosa. Anamnestic charts designed for this study included information about symptoms (measured by Visual Analogue Scale), previous knowledge or fear about presence of GT and received therapy for this condition. Detection of Candida spp. from tongue was done using sterile cotton swab and Sabouraud dextrose agar. Results: At the time of this study, 18 (52.94%) of subjects with GT were aware of having this condition and even 12 of them (66.7%) used topical antifungal drugs prescribed by physician. Also, 66.7% of them experienced fear about the presence of GT at some moment. Detection of Candida spp. was similarly distributed in both groups (22.2% in group A and 17.6% in group B). Conclusions: Geographic tongue has frequently been treated by topical antifungal drugs. In this study, GT was not associated with presence of Candida spp.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Dental Medicine
T1  - Geographic tongue: Does Candida play a role in its pathogenesis
VL  - 23
IS  - 3
SP  - 152
EP  - 156
DO  - 10.2478/bjdm-2019-0027
ER  - 

@article{
author = "Matić-Petrović, Sanja and Đorđević, Mihajlo and Radunović, Milena and Živanović, Tanja and Pavlica, Dušan and Pucar, Ana",
year = "2019",
abstract = "Background/Aim: Geographic tongue (GT), a benign self-limiting condition (inflammation) is commonly seen in practice. Although quite easy for clinical diagnosis, in clinical practice it is commonly misdiagnosed and treated as Candida infection. The main aim of this study was to compare the prevalence of Candida spp. on tongue in patients with GT and subjects with clinically healthy oral mucosa. Additional aims were to evaluate subjective symptoms, uncontrolled usage of prescribed or non-prescribed antifungal topical medications and the presence of the cancerophobia or other health concerns in patients with GT. Material and Methods: A total of 70 subjects were divided into two groups: Group B - patients with diagnosed Geographic tongue and group A - aged and gender matched controls with clinically health oral mucosa. Anamnestic charts designed for this study included information about symptoms (measured by Visual Analogue Scale), previous knowledge or fear about presence of GT and received therapy for this condition. Detection of Candida spp. from tongue was done using sterile cotton swab and Sabouraud dextrose agar. Results: At the time of this study, 18 (52.94%) of subjects with GT were aware of having this condition and even 12 of them (66.7%) used topical antifungal drugs prescribed by physician. Also, 66.7% of them experienced fear about the presence of GT at some moment. Detection of Candida spp. was similarly distributed in both groups (22.2% in group A and 17.6% in group B). Conclusions: Geographic tongue has frequently been treated by topical antifungal drugs. In this study, GT was not associated with presence of Candida spp.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Dental Medicine",
title = "Geographic tongue: Does Candida play a role in its pathogenesis",
volume = "23",
number = "3",
pages = "152-156",
doi = "10.2478/bjdm-2019-0027"
}

Matić-Petrović, S., Đorđević, M., Radunović, M., Živanović, T., Pavlica, D.,& Pucar, A.. (2019). Geographic tongue: Does Candida play a role in its pathogenesis. in Balkan Journal of Dental Medicine
Udruženje stomatologa Balkana., 23(3), 152-156.
https://doi.org/10.2478/bjdm-2019-0027

Matić-Petrović S, Đorđević M, Radunović M, Živanović T, Pavlica D, Pucar A. Geographic tongue: Does Candida play a role in its pathogenesis. in Balkan Journal of Dental Medicine. 2019;23(3):152-156.
doi:10.2478/bjdm-2019-0027 .

Matić-Petrović, Sanja, Đorđević, Mihajlo, Radunović, Milena, Živanović, Tanja, Pavlica, Dušan, Pucar, Ana, "Geographic tongue: Does Candida play a role in its pathogenesis" in Balkan Journal of Dental Medicine, 23, no. 3 (2019):152-156,
https://doi.org/10.2478/bjdm-2019-0027 . .

TY  - JOUR
AU  - Kannosh, Ibrahim
AU  - Staletović, Danijela
AU  - Toljić, Boško
AU  - Radunović, Milena
AU  - Pucar, Ana
AU  - Matić-Petrović, Sanja
AU  - Grubiša, Ivana
AU  - Lazarević, Miloš
AU  - Brkić, Zlata
AU  - Knežević-Vukcević, Jelena
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2276
AB  - Introduction: There is a known connection between periodontitis and atherosclerosis and the presence of periopathogens in blood vessels. However, changes of the oral microflora related to the aging process and its possible effects on atherosclerosis, have yet to be analyzed. The aim of this study was to assess temporal changes in the frequency of periodontal bacteria in the subgingival plaque and in atherosclerotic blood vessels of patients with atherosclerosis. Methodology: The study included 100 patients with atherosclerosis and periodontitis, divided into two groups, below and over 60 years of age. Clinical examinations were performedand subgingival plaque specimens were collected as well as biopsy specimens from the following arteries: coronary (34), carotid (29), abdominal (10), femoral (10), mammary (13) and iliac (4). Subgingival and artery specimens were subjected to PCR detection of 5 major periodontal pathogens: Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Aggregatibacter actinomycetemcomitans (Aa), Tannerella forsythensis (Tf) and Treponema denticola (Td). Results: Tf was the most and Td the least frequent bacteria in both age groups and in both types of samples. The frequencies of bacteria in subgingival versus atherosclerotic samples were: Tf (76%: 53%), Pi (71%: 31%), Pg (60%: 38%), Aa (39%: 14%) and Td (21%: 6%). Only Aa and Pi showed a significant difference of prevalence between younger and older patients. The most colonized artery was a. coronaria, followed by a. carotis, a. abdominalis, a. mammaria, and a. femoralis. Conclusions: Patient's age and the distance of a given blood vessel from the oral cavity influenced microbiological findings in the atherotic plaque.
PB  - J Infection Developing Countries, Tramaniglio
T2  - Journal of Infection in Developing Countries
T1  - The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis
VL  - 12
IS  - 12
SP  - 1088
EP  - 1095
DO  - 10.3855/jidc.10980
ER  - 

@article{
author = "Kannosh, Ibrahim and Staletović, Danijela and Toljić, Boško and Radunović, Milena and Pucar, Ana and Matić-Petrović, Sanja and Grubiša, Ivana and Lazarević, Miloš and Brkić, Zlata and Knežević-Vukcević, Jelena and Milašin, Jelena",
year = "2018",
abstract = "Introduction: There is a known connection between periodontitis and atherosclerosis and the presence of periopathogens in blood vessels. However, changes of the oral microflora related to the aging process and its possible effects on atherosclerosis, have yet to be analyzed. The aim of this study was to assess temporal changes in the frequency of periodontal bacteria in the subgingival plaque and in atherosclerotic blood vessels of patients with atherosclerosis. Methodology: The study included 100 patients with atherosclerosis and periodontitis, divided into two groups, below and over 60 years of age. Clinical examinations were performedand subgingival plaque specimens were collected as well as biopsy specimens from the following arteries: coronary (34), carotid (29), abdominal (10), femoral (10), mammary (13) and iliac (4). Subgingival and artery specimens were subjected to PCR detection of 5 major periodontal pathogens: Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Aggregatibacter actinomycetemcomitans (Aa), Tannerella forsythensis (Tf) and Treponema denticola (Td). Results: Tf was the most and Td the least frequent bacteria in both age groups and in both types of samples. The frequencies of bacteria in subgingival versus atherosclerotic samples were: Tf (76%: 53%), Pi (71%: 31%), Pg (60%: 38%), Aa (39%: 14%) and Td (21%: 6%). Only Aa and Pi showed a significant difference of prevalence between younger and older patients. The most colonized artery was a. coronaria, followed by a. carotis, a. abdominalis, a. mammaria, and a. femoralis. Conclusions: Patient's age and the distance of a given blood vessel from the oral cavity influenced microbiological findings in the atherotic plaque.",
publisher = "J Infection Developing Countries, Tramaniglio",
journal = "Journal of Infection in Developing Countries",
title = "The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis",
volume = "12",
number = "12",
pages = "1088-1095",
doi = "10.3855/jidc.10980"
}

Kannosh, I., Staletović, D., Toljić, B., Radunović, M., Pucar, A., Matić-Petrović, S., Grubiša, I., Lazarević, M., Brkić, Z., Knežević-Vukcević, J.,& Milašin, J.. (2018). The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis. in Journal of Infection in Developing Countries
J Infection Developing Countries, Tramaniglio., 12(12), 1088-1095.
https://doi.org/10.3855/jidc.10980

Kannosh I, Staletović D, Toljić B, Radunović M, Pucar A, Matić-Petrović S, Grubiša I, Lazarević M, Brkić Z, Knežević-Vukcević J, Milašin J. The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis. in Journal of Infection in Developing Countries. 2018;12(12):1088-1095.
doi:10.3855/jidc.10980 .

Kannosh, Ibrahim, Staletović, Danijela, Toljić, Boško, Radunović, Milena, Pucar, Ana, Matić-Petrović, Sanja, Grubiša, Ivana, Lazarević, Miloš, Brkić, Zlata, Knežević-Vukcević, Jelena, Milašin, Jelena, "The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis" in Journal of Infection in Developing Countries, 12, no. 12 (2018):1088-1095,
https://doi.org/10.3855/jidc.10980 . .

TY  - CONF
AU  - Radunović, Milena
AU  - Matić-Petrović, Sanja
AU  - Cimbaljević, Milena
AU  - Kuzmanović-Pfićer, Jovana
AU  - Pavlica, Dušan
AU  - Arsić-Arsenijević, Valentina
AU  - Pucar, Ana
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2295
PB  - Oxford Univ Press, Oxford
C3  - Medical Mycology
T1  - Subgingival sites and oral cavity as reservoirs for Candida spp. in diabetics and non-diabetics
VL  - 56
SP  - S108
EP  - S108
UR  - https://hdl.handle.net/21.15107/rcub_smile_2295
ER  - 

@conference{
author = "Radunović, Milena and Matić-Petrović, Sanja and Cimbaljević, Milena and Kuzmanović-Pfićer, Jovana and Pavlica, Dušan and Arsić-Arsenijević, Valentina and Pucar, Ana",
year = "2018",
publisher = "Oxford Univ Press, Oxford",
journal = "Medical Mycology",
title = "Subgingival sites and oral cavity as reservoirs for Candida spp. in diabetics and non-diabetics",
volume = "56",
pages = "S108-S108",
url = "https://hdl.handle.net/21.15107/rcub_smile_2295"
}

Radunović, M., Matić-Petrović, S., Cimbaljević, M., Kuzmanović-Pfićer, J., Pavlica, D., Arsić-Arsenijević, V.,& Pucar, A.. (2018). Subgingival sites and oral cavity as reservoirs for Candida spp. in diabetics and non-diabetics. in Medical Mycology
Oxford Univ Press, Oxford., 56, S108-S108.
https://hdl.handle.net/21.15107/rcub_smile_2295

Radunović M, Matić-Petrović S, Cimbaljević M, Kuzmanović-Pfićer J, Pavlica D, Arsić-Arsenijević V, Pucar A. Subgingival sites and oral cavity as reservoirs for Candida spp. in diabetics and non-diabetics. in Medical Mycology. 2018;56:S108-S108.
https://hdl.handle.net/21.15107/rcub_smile_2295 .

Radunović, Milena, Matić-Petrović, Sanja, Cimbaljević, Milena, Kuzmanović-Pfićer, Jovana, Pavlica, Dušan, Arsić-Arsenijević, Valentina, Pucar, Ana, "Subgingival sites and oral cavity as reservoirs for Candida spp. in diabetics and non-diabetics" in Medical Mycology, 56 (2018):S108-S108,
https://hdl.handle.net/21.15107/rcub_smile_2295 .

TY  - JOUR
AU  - Matić-Petrović, Sanja
AU  - Barać, Milena
AU  - Kuzmanović-Pfićer, Jovana
AU  - Radunović, Milena
AU  - Jotić, Aleksandra
AU  - Pucar, Ana
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2365
AB  - Background/Aim: The aim of this study was to examine prevalence of different Candida spp. at diabetics and nondiabetics wearing dentures without clinical signs of Denture Stomatitis (DS) and to study if some local and systematic factors are confounders for harboring Candida at these subjects. Material and Methods: Total of 60 subjects wearing partial or complete upper acrylic denture having at least half of palatal mucosa covered by denture were selected and stratified into three experimental groups: systematically health subjects; patients with diagnosed Type 2 Diabetes (T2D) and good glycoregulation; and T2D subjects with poorly regulated blood sugar level. Cotton swab samples were obtained from each patient from hard palate mucosa and denture surface. Swab cultures were made on Sabouraud dextrose agar and ChromAgar Media for distinciton of various Candida spp. Density growth was also measured. Results: Frequency of Candida spp. findings were similar between groups. At healthy subjects, only C.albicans was detected. At diabetics, C.albicans was the most common isolated species, followed by C.glabrata and C.tropicalis. Negative finding of yeasts on palatal mucosa, but positive on denture surface were detected at all groups, with the highest frequency (33.4%) at diabetics with poor glycoregulation. Denture surface was heavier colonized than hard palate mucosa. Duration of diabetes in years were only independent predictors for harboring Candida spp. at denture surface (Exp B=1.186, CI=1.047-1.344, p=0.007). Conclusions: Prosthesis of denture wearers without DS may serve as reservoir of Candida spp. Presence of more pathogenic and resistant non-albicans species are related to diabetics, even without clinical signs of DS.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Dental Medicine
T1  - Presence of different Candida species at denture wearers with type 2 diabetes and clinically healthy oral mucosa: Pilot study
VL  - 22
IS  - 1
SP  - 15
EP  - 21
DO  - 10.2478/bjdm-2018-0003
ER  - 

@article{
author = "Matić-Petrović, Sanja and Barać, Milena and Kuzmanović-Pfićer, Jovana and Radunović, Milena and Jotić, Aleksandra and Pucar, Ana",
year = "2018",
abstract = "Background/Aim: The aim of this study was to examine prevalence of different Candida spp. at diabetics and nondiabetics wearing dentures without clinical signs of Denture Stomatitis (DS) and to study if some local and systematic factors are confounders for harboring Candida at these subjects. Material and Methods: Total of 60 subjects wearing partial or complete upper acrylic denture having at least half of palatal mucosa covered by denture were selected and stratified into three experimental groups: systematically health subjects; patients with diagnosed Type 2 Diabetes (T2D) and good glycoregulation; and T2D subjects with poorly regulated blood sugar level. Cotton swab samples were obtained from each patient from hard palate mucosa and denture surface. Swab cultures were made on Sabouraud dextrose agar and ChromAgar Media for distinciton of various Candida spp. Density growth was also measured. Results: Frequency of Candida spp. findings were similar between groups. At healthy subjects, only C.albicans was detected. At diabetics, C.albicans was the most common isolated species, followed by C.glabrata and C.tropicalis. Negative finding of yeasts on palatal mucosa, but positive on denture surface were detected at all groups, with the highest frequency (33.4%) at diabetics with poor glycoregulation. Denture surface was heavier colonized than hard palate mucosa. Duration of diabetes in years were only independent predictors for harboring Candida spp. at denture surface (Exp B=1.186, CI=1.047-1.344, p=0.007). Conclusions: Prosthesis of denture wearers without DS may serve as reservoir of Candida spp. Presence of more pathogenic and resistant non-albicans species are related to diabetics, even without clinical signs of DS.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Dental Medicine",
title = "Presence of different Candida species at denture wearers with type 2 diabetes and clinically healthy oral mucosa: Pilot study",
volume = "22",
number = "1",
pages = "15-21",
doi = "10.2478/bjdm-2018-0003"
}

Matić-Petrović, S., Barać, M., Kuzmanović-Pfićer, J., Radunović, M., Jotić, A.,& Pucar, A.. (2018). Presence of different Candida species at denture wearers with type 2 diabetes and clinically healthy oral mucosa: Pilot study. in Balkan Journal of Dental Medicine
Udruženje stomatologa Balkana., 22(1), 15-21.
https://doi.org/10.2478/bjdm-2018-0003

Matić-Petrović S, Barać M, Kuzmanović-Pfićer J, Radunović M, Jotić A, Pucar A. Presence of different Candida species at denture wearers with type 2 diabetes and clinically healthy oral mucosa: Pilot study. in Balkan Journal of Dental Medicine. 2018;22(1):15-21.
doi:10.2478/bjdm-2018-0003 .

Matić-Petrović, Sanja, Barać, Milena, Kuzmanović-Pfićer, Jovana, Radunović, Milena, Jotić, Aleksandra, Pucar, Ana, "Presence of different Candida species at denture wearers with type 2 diabetes and clinically healthy oral mucosa: Pilot study" in Balkan Journal of Dental Medicine, 22, no. 1 (2018):15-21,
https://doi.org/10.2478/bjdm-2018-0003 . .

TY  - CONF
AU  - Matić-Petrović, Sanja
AU  - Jovelić, N.
AU  - Toljić, Boško
AU  - Đorđević, M.
AU  - Pucar, Ana
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2152
PB  - Wiley, Hoboken
C3  - Oral Diseases
T1  - TNFRs polymorphism and serum concentrations in periodontitis and diabetes
VL  - 22
SP  - 33
EP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_smile_2152
ER  - 

@conference{
author = "Matić-Petrović, Sanja and Jovelić, N. and Toljić, Boško and Đorđević, M. and Pucar, Ana",
year = "2016",
publisher = "Wiley, Hoboken",
journal = "Oral Diseases",
title = "TNFRs polymorphism and serum concentrations in periodontitis and diabetes",
volume = "22",
pages = "33-33",
url = "https://hdl.handle.net/21.15107/rcub_smile_2152"
}

Matić-Petrović, S., Jovelić, N., Toljić, B., Đorđević, M.,& Pucar, A.. (2016). TNFRs polymorphism and serum concentrations in periodontitis and diabetes. in Oral Diseases
Wiley, Hoboken., 22, 33-33.
https://hdl.handle.net/21.15107/rcub_smile_2152

Matić-Petrović S, Jovelić N, Toljić B, Đorđević M, Pucar A. TNFRs polymorphism and serum concentrations in periodontitis and diabetes. in Oral Diseases. 2016;22:33-33.
https://hdl.handle.net/21.15107/rcub_smile_2152 .

Matić-Petrović, Sanja, Jovelić, N., Toljić, Boško, Đorđević, M., Pucar, Ana, "TNFRs polymorphism and serum concentrations in periodontitis and diabetes" in Oral Diseases, 22 (2016):33-33,
https://hdl.handle.net/21.15107/rcub_smile_2152 .

TY  - JOUR
AU  - Matić-Petrović, Sanja
AU  - Pucar, Ana
AU  - Jotić, Aleksandra
AU  - Miličić, Biljana
AU  - Arambašić-Jovanović, Jelena
AU  - Vidaković, Melita
AU  - Leković, Vojislav
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2158
AB  - Introduction: The role of tumor necrosis factor-α (TNFα) is well documented in pathogenesis of chronic periodontitis (CP) and type 2 diabetes (T2D). Considering short half-life of TNFα, tumor necrosis factor receptor-2 (TNFR2) is used as prosperous surrogate marker of TNFα activity. Objective The aim was to detect TNFR2 serum concentration and correlate it with periodontal destruction in patients with diagnosed T2D and nondiabetics. Methods The study included 85 patients divided into three groups: T2D + CP (group T2D, n = 34); nondiabetics + CP (Group PD, n = 27); and healthy controls (group HC, n = 24). T2D was diagnosed according to WHO criteria (2013) and periodontitis was diagnosed using International Workshop for a Classification of Periodontal Diseases and Conditions criteria (1999). TNFR2 level was measured by enzyme-linked immunosorbent assay (ELISA). Results There was no difference in TNFR2 level among the groups (Kruskal-Wallis, p = 0.482). Significant correlation (Pearson's correlation coefficient) was observed between clinical attachment loss (CAL) and TNFR2 concentration in PD group (rp = -0.460, p = 0.016). In T2D group, correlations were observed between TNFR2 concentration and CaL (rp = 0.363, p = 0.005) and periodontal inflamed surface area (PISA) (rp = 0.345, p = 0.046) and periodontalepithelial surface area (PESA) (rp = 0.578, p = 0.000). Conclusion Higher concentration of TNFR2 was associated with higher CAL, PESA, and PISA scores in T2D group. Contrary to that, nondiabetics with higher values of CAL exhibited lower concentration of TNFR2, presenting potential protective effect on periodontal destruction. These results imply that diabetes may alter TNFR2 secretion originated from periodontium.
AB  - Uvod: Uloga faktora nekroze tumora-alfa (TNFα) dokazana je u patogenezi hronične parodontopatije (HP) i dijabetesa melitusa tipa 2 (DM tip 2). S obzirom na to da je poluživot TNFα veoma kratak, receptor 2 faktora nekroze tumora (TNFR2) koristi se kao marker aktivnosti TNFα. Cilj rada Cilj ovog rada je određivanje koncentracije TNFR2 u serumu i koreliranje sa parametrima destrukcije parodoncijuma kod zdravih i ispitanika sa dijagnostikovanim DM tip 2. Metode rada U studiju je uključeno 85 pacijenata podeljenih u tri grupe: DM tip 2 + HP (DM grupa, n = 34), zdravi ispitanici + HP (PD grupa, n = 27) i zdrave kontrole (ZK grupa, n = 24). Dijagnoza DM tip 2 postavljena je na osnovu kriterijuma SZO (2013), dok je dijagnoza HP postavljena na osnovu kriterijuma Internacionalne radionice za klasifikaciju stanja i oboljenja parodoncijuma (1999). Koncentracija TNFR2 merena je ELISA metodom. Rezultati Koncentracija serumskog TNFR2 nije se razlikovala među grupama (Kraskal-Volis, p = 0,482). Postoji značajna korelacija (Pirson) između nivoa pripojnog epitela (NPE) i koncentracije TNFR2 u PD grupi (rp = -0,460, p = 0,016). U DM tip 2 grupi, statistički značajna korelacija uočena je između koncentracije TNFR2 i NPE (rp = 0,363, p = 0,005), kao i parametara uticaja inflamacije iz parodoncijuma na sistemsko zdravlje - PISA (rp = 0,345, p = 0,046) i PESA (rp = 0,578, p = 0,000). Zaključak Kod pacijenata sa dijabetesom veće koncentracije TNFR2 odgovaraju većim vrednostima NPE, PESA i PISA. Nasuprot tome, kod sistemski zdravih ispitanika sa HP veće vrednosti NPE su povezane sa manjim koncentracijama TNFR2, što bi moglo govoriti o potencijalnoj zaštitnoj ulozi ovog citokina na destrukciju parodoncijuma. Rezultati govore da dijabetes može uticati na sekreciju TNFR2 iz parodoncijuma.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study
T1  - Korelacija između koncentracije receptora 2 faktora nekroze tumora u serumu i destrukcije parodoncijuma kod bolesnika sa dijabetes melitusom tip 2 - studija preseka
VL  - 144
IS  - 5-6
SP  - 266
EP  - 272
DO  - 10.2298/SARH1606266M
ER  - 

@article{
author = "Matić-Petrović, Sanja and Pucar, Ana and Jotić, Aleksandra and Miličić, Biljana and Arambašić-Jovanović, Jelena and Vidaković, Melita and Leković, Vojislav",
year = "2016",
abstract = "Introduction: The role of tumor necrosis factor-α (TNFα) is well documented in pathogenesis of chronic periodontitis (CP) and type 2 diabetes (T2D). Considering short half-life of TNFα, tumor necrosis factor receptor-2 (TNFR2) is used as prosperous surrogate marker of TNFα activity. Objective The aim was to detect TNFR2 serum concentration and correlate it with periodontal destruction in patients with diagnosed T2D and nondiabetics. Methods The study included 85 patients divided into three groups: T2D + CP (group T2D, n = 34); nondiabetics + CP (Group PD, n = 27); and healthy controls (group HC, n = 24). T2D was diagnosed according to WHO criteria (2013) and periodontitis was diagnosed using International Workshop for a Classification of Periodontal Diseases and Conditions criteria (1999). TNFR2 level was measured by enzyme-linked immunosorbent assay (ELISA). Results There was no difference in TNFR2 level among the groups (Kruskal-Wallis, p = 0.482). Significant correlation (Pearson's correlation coefficient) was observed between clinical attachment loss (CAL) and TNFR2 concentration in PD group (rp = -0.460, p = 0.016). In T2D group, correlations were observed between TNFR2 concentration and CaL (rp = 0.363, p = 0.005) and periodontal inflamed surface area (PISA) (rp = 0.345, p = 0.046) and periodontalepithelial surface area (PESA) (rp = 0.578, p = 0.000). Conclusion Higher concentration of TNFR2 was associated with higher CAL, PESA, and PISA scores in T2D group. Contrary to that, nondiabetics with higher values of CAL exhibited lower concentration of TNFR2, presenting potential protective effect on periodontal destruction. These results imply that diabetes may alter TNFR2 secretion originated from periodontium., Uvod: Uloga faktora nekroze tumora-alfa (TNFα) dokazana je u patogenezi hronične parodontopatije (HP) i dijabetesa melitusa tipa 2 (DM tip 2). S obzirom na to da je poluživot TNFα veoma kratak, receptor 2 faktora nekroze tumora (TNFR2) koristi se kao marker aktivnosti TNFα. Cilj rada Cilj ovog rada je određivanje koncentracije TNFR2 u serumu i koreliranje sa parametrima destrukcije parodoncijuma kod zdravih i ispitanika sa dijagnostikovanim DM tip 2. Metode rada U studiju je uključeno 85 pacijenata podeljenih u tri grupe: DM tip 2 + HP (DM grupa, n = 34), zdravi ispitanici + HP (PD grupa, n = 27) i zdrave kontrole (ZK grupa, n = 24). Dijagnoza DM tip 2 postavljena je na osnovu kriterijuma SZO (2013), dok je dijagnoza HP postavljena na osnovu kriterijuma Internacionalne radionice za klasifikaciju stanja i oboljenja parodoncijuma (1999). Koncentracija TNFR2 merena je ELISA metodom. Rezultati Koncentracija serumskog TNFR2 nije se razlikovala među grupama (Kraskal-Volis, p = 0,482). Postoji značajna korelacija (Pirson) između nivoa pripojnog epitela (NPE) i koncentracije TNFR2 u PD grupi (rp = -0,460, p = 0,016). U DM tip 2 grupi, statistički značajna korelacija uočena je između koncentracije TNFR2 i NPE (rp = 0,363, p = 0,005), kao i parametara uticaja inflamacije iz parodoncijuma na sistemsko zdravlje - PISA (rp = 0,345, p = 0,046) i PESA (rp = 0,578, p = 0,000). Zaključak Kod pacijenata sa dijabetesom veće koncentracije TNFR2 odgovaraju većim vrednostima NPE, PESA i PISA. Nasuprot tome, kod sistemski zdravih ispitanika sa HP veće vrednosti NPE su povezane sa manjim koncentracijama TNFR2, što bi moglo govoriti o potencijalnoj zaštitnoj ulozi ovog citokina na destrukciju parodoncijuma. Rezultati govore da dijabetes može uticati na sekreciju TNFR2 iz parodoncijuma.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study, Korelacija između koncentracije receptora 2 faktora nekroze tumora u serumu i destrukcije parodoncijuma kod bolesnika sa dijabetes melitusom tip 2 - studija preseka",
volume = "144",
number = "5-6",
pages = "266-272",
doi = "10.2298/SARH1606266M"
}

Matić-Petrović, S., Pucar, A., Jotić, A., Miličić, B., Arambašić-Jovanović, J., Vidaković, M.,& Leković, V.. (2016). Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 144(5-6), 266-272.
https://doi.org/10.2298/SARH1606266M

Matić-Petrović S, Pucar A, Jotić A, Miličić B, Arambašić-Jovanović J, Vidaković M, Leković V. Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study. in Srpski arhiv za celokupno lekarstvo. 2016;144(5-6):266-272.
doi:10.2298/SARH1606266M .

Matić-Petrović, Sanja, Pucar, Ana, Jotić, Aleksandra, Miličić, Biljana, Arambašić-Jovanović, Jelena, Vidaković, Melita, Leković, Vojislav, "Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study" in Srpski arhiv za celokupno lekarstvo, 144, no. 5-6 (2016):266-272,
https://doi.org/10.2298/SARH1606266M . .

TY  - JOUR
AU  - Grdović, Nevena
AU  - Rajić, Jovana
AU  - Matić-Petrović, Sanja
AU  - Dinić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Mihailović, Mirjana
AU  - Arambašić-Jovanović, Jelena
AU  - Tolić, Anja
AU  - Pucar, Ana
AU  - Milašin, Jelena
AU  - Vidaković, Melita
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2122
AB  - Objectives: CXCL12 is widely expressed, constitutive chemokine involved in tissue repair and regeneration, while the extent of its expression is important in various chronic inflammatory conditions. Involvement of DNA methylation in CXCL12 gene suppression (CXCL12) has been shown in malignancy and some autoimmune diseases. The aim of this study was to investigate whether the alterations in DNA methylation of CXCL12 are also involved in progression of periodontitis in combination with diabetes, as these chronic inflammatory conditions are strongly interrelated. Design: Study included 72 subjects divided in three groups: healthy control (C, n=21), periodontitis (P, n=29) and diabetes/periodontitis group (D/P, n=22). DNA extracted from epithelial cells obtained by sterile cotton swabs from buccal mucosa was subjected to methylation specific polymerase chain reaction (MSP) to obtain DNA methylation pattern of CXCL12 promoter. Results: CXCL12 promoter was predominantly unmethylated in all groups. However, increase in the frequency of the methylated form and increase in percent of methylation of CXCL12 promoter in periodontitis and diabetes/periodontitis group compared to control group were found, although without statistical significance. However, statistically significant increase in Tm of MSP products in diabetes/periodontitis group was observed. Correlation analysis revealed statistically significant relationship between the extent of DNA methylation of the CXCL12 promoter and periodontal parameters, as well as between DNA methylation of CXCL12 and glycosylated hemoglobin. Conclusion: Presented results suggest that chronic inflammation contributes to the change of CXCL12 DNA methylation in buccal cells and that DNA methylation profile of CXCL12 promoter plays important role in development and progression of periodontal disease.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Archives of Oral Biology
T1  - Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2
VL  - 72
SP  - 124
EP  - 133
DO  - 10.1016/j.archoralbio.2016.08.025
ER  - 

@article{
author = "Grdović, Nevena and Rajić, Jovana and Matić-Petrović, Sanja and Dinić, Svetlana and Uskoković, Aleksandra and Mihailović, Mirjana and Arambašić-Jovanović, Jelena and Tolić, Anja and Pucar, Ana and Milašin, Jelena and Vidaković, Melita",
year = "2016",
abstract = "Objectives: CXCL12 is widely expressed, constitutive chemokine involved in tissue repair and regeneration, while the extent of its expression is important in various chronic inflammatory conditions. Involvement of DNA methylation in CXCL12 gene suppression (CXCL12) has been shown in malignancy and some autoimmune diseases. The aim of this study was to investigate whether the alterations in DNA methylation of CXCL12 are also involved in progression of periodontitis in combination with diabetes, as these chronic inflammatory conditions are strongly interrelated. Design: Study included 72 subjects divided in three groups: healthy control (C, n=21), periodontitis (P, n=29) and diabetes/periodontitis group (D/P, n=22). DNA extracted from epithelial cells obtained by sterile cotton swabs from buccal mucosa was subjected to methylation specific polymerase chain reaction (MSP) to obtain DNA methylation pattern of CXCL12 promoter. Results: CXCL12 promoter was predominantly unmethylated in all groups. However, increase in the frequency of the methylated form and increase in percent of methylation of CXCL12 promoter in periodontitis and diabetes/periodontitis group compared to control group were found, although without statistical significance. However, statistically significant increase in Tm of MSP products in diabetes/periodontitis group was observed. Correlation analysis revealed statistically significant relationship between the extent of DNA methylation of the CXCL12 promoter and periodontal parameters, as well as between DNA methylation of CXCL12 and glycosylated hemoglobin. Conclusion: Presented results suggest that chronic inflammation contributes to the change of CXCL12 DNA methylation in buccal cells and that DNA methylation profile of CXCL12 promoter plays important role in development and progression of periodontal disease.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Archives of Oral Biology",
title = "Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2",
volume = "72",
pages = "124-133",
doi = "10.1016/j.archoralbio.2016.08.025"
}

Grdović, N., Rajić, J., Matić-Petrović, S., Dinić, S., Uskoković, A., Mihailović, M., Arambašić-Jovanović, J., Tolić, A., Pucar, A., Milašin, J.,& Vidaković, M.. (2016). Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2. in Archives of Oral Biology
Pergamon-Elsevier Science Ltd, Oxford., 72, 124-133.
https://doi.org/10.1016/j.archoralbio.2016.08.025

Grdović N, Rajić J, Matić-Petrović S, Dinić S, Uskoković A, Mihailović M, Arambašić-Jovanović J, Tolić A, Pucar A, Milašin J, Vidaković M. Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2. in Archives of Oral Biology. 2016;72:124-133.
doi:10.1016/j.archoralbio.2016.08.025 .

Grdović, Nevena, Rajić, Jovana, Matić-Petrović, Sanja, Dinić, Svetlana, Uskoković, Aleksandra, Mihailović, Mirjana, Arambašić-Jovanović, Jelena, Tolić, Anja, Pucar, Ana, Milašin, Jelena, Vidaković, Melita, "Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2" in Archives of Oral Biology, 72 (2016):124-133,
https://doi.org/10.1016/j.archoralbio.2016.08.025 . .

TY  - JOUR
AU  - Matić-Petrović, Sanja
AU  - Cimbaljević, Milena
AU  - Radunović, Milena
AU  - Kuzmanović-Pfićer, Jovana
AU  - Jotić, Aleksandra
AU  - Pucar, Ana
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2052
AB  - The purpose of this study was to detect Candida spp. on the tongue and in the subgingival sites in healthy and type 2 diabetes (T2D) patients with chronic periodontitis (CP), and to compare the accuracy of sampling methods. This study included 131 patients divided into four groups: healthy control (group A), nondiabetics + CP (Group B), diabetics with good metabolic control + CP (group C) and diabetics with poor glycoregulation + CP (Group D). Cotton swab samples from tongue and subgingival samples were obtained from each patient with help of sterile paper points and a sterile curette. Swab cultures were made on Sabouraud dextrose agar. The number of CFUs was counted. The sampling methods for subgingival plaque were compared by Receiving Operator Curve (ROC). The presence of Candida spp. on the tongue was statistically significant among groups (group D vs. others three groups:.2: p  lt  0.005 for each group). Positive findings of subgingival Candida spp. did not differ among the groups. There were no significant differences in the quantification of Candida spp., neither on the tongue, nor in the subgingival samples. 17.2% of diabetic patients revealed the presence of Candida spp. in the subgingival samples, with negative finding on tongue. There was a significant difference in the sampling methods for subgingival plaque (p = 0.000). Candida spp. is more prevalent on the tongue of diabetics. The sampling of subgingival plaque by a sterile curette is more accurate than with paper points. Subgingival plaque may represent a reservoir of commensals. It is necessary to standardize the sampling of subgingival plaque.
PB  - Sociedade Brasileira De Pesquisa Odontologica, Sao Paulo
T2  - Brazilian Oral Research
T1  - Detection and sampling methods for isolation of Candida spp. from oral cavities in diabetics and non-diabetics
VL  - 29
IS  - 1
DO  - 10.1590/1807-3107BOR-2015.vol29.0077
ER  - 

@article{
author = "Matić-Petrović, Sanja and Cimbaljević, Milena and Radunović, Milena and Kuzmanović-Pfićer, Jovana and Jotić, Aleksandra and Pucar, Ana",
year = "2015",
abstract = "The purpose of this study was to detect Candida spp. on the tongue and in the subgingival sites in healthy and type 2 diabetes (T2D) patients with chronic periodontitis (CP), and to compare the accuracy of sampling methods. This study included 131 patients divided into four groups: healthy control (group A), nondiabetics + CP (Group B), diabetics with good metabolic control + CP (group C) and diabetics with poor glycoregulation + CP (Group D). Cotton swab samples from tongue and subgingival samples were obtained from each patient with help of sterile paper points and a sterile curette. Swab cultures were made on Sabouraud dextrose agar. The number of CFUs was counted. The sampling methods for subgingival plaque were compared by Receiving Operator Curve (ROC). The presence of Candida spp. on the tongue was statistically significant among groups (group D vs. others three groups:.2: p  lt  0.005 for each group). Positive findings of subgingival Candida spp. did not differ among the groups. There were no significant differences in the quantification of Candida spp., neither on the tongue, nor in the subgingival samples. 17.2% of diabetic patients revealed the presence of Candida spp. in the subgingival samples, with negative finding on tongue. There was a significant difference in the sampling methods for subgingival plaque (p = 0.000). Candida spp. is more prevalent on the tongue of diabetics. The sampling of subgingival plaque by a sterile curette is more accurate than with paper points. Subgingival plaque may represent a reservoir of commensals. It is necessary to standardize the sampling of subgingival plaque.",
publisher = "Sociedade Brasileira De Pesquisa Odontologica, Sao Paulo",
journal = "Brazilian Oral Research",
title = "Detection and sampling methods for isolation of Candida spp. from oral cavities in diabetics and non-diabetics",
volume = "29",
number = "1",
doi = "10.1590/1807-3107BOR-2015.vol29.0077"
}

Matić-Petrović, S., Cimbaljević, M., Radunović, M., Kuzmanović-Pfićer, J., Jotić, A.,& Pucar, A.. (2015). Detection and sampling methods for isolation of Candida spp. from oral cavities in diabetics and non-diabetics. in Brazilian Oral Research
Sociedade Brasileira De Pesquisa Odontologica, Sao Paulo., 29(1).
https://doi.org/10.1590/1807-3107BOR-2015.vol29.0077

Matić-Petrović S, Cimbaljević M, Radunović M, Kuzmanović-Pfićer J, Jotić A, Pucar A. Detection and sampling methods for isolation of Candida spp. from oral cavities in diabetics and non-diabetics. in Brazilian Oral Research. 2015;29(1).
doi:10.1590/1807-3107BOR-2015.vol29.0077 .

Matić-Petrović, Sanja, Cimbaljević, Milena, Radunović, Milena, Kuzmanović-Pfićer, Jovana, Jotić, Aleksandra, Pucar, Ana, "Detection and sampling methods for isolation of Candida spp. from oral cavities in diabetics and non-diabetics" in Brazilian Oral Research, 29, no. 1 (2015),
https://doi.org/10.1590/1807-3107BOR-2015.vol29.0077 . .

TY  - CONF
AU  - Matić-Petrović, Sanja
AU  - Milašin, Jelena
AU  - Vidaković, M.
AU  - Jotić, Aleksandra
AU  - Toljić, Boško
AU  - Miličić, Biljana
AU  - Pucar, Ana
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1933
PB  - Wiley-Blackwell, Hoboken
C3  - Oral Diseases
T1  - TNFR2 polymorphism and serum concentrations in periodontitis and diabetes
VL  - 20
SP  - 19
EP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_smile_1933
ER  - 

@conference{
author = "Matić-Petrović, Sanja and Milašin, Jelena and Vidaković, M. and Jotić, Aleksandra and Toljić, Boško and Miličić, Biljana and Pucar, Ana",
year = "2014",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Oral Diseases",
title = "TNFR2 polymorphism and serum concentrations in periodontitis and diabetes",
volume = "20",
pages = "19-19",
url = "https://hdl.handle.net/21.15107/rcub_smile_1933"
}

Matić-Petrović, S., Milašin, J., Vidaković, M., Jotić, A., Toljić, B., Miličić, B.,& Pucar, A.. (2014). TNFR2 polymorphism and serum concentrations in periodontitis and diabetes. in Oral Diseases
Wiley-Blackwell, Hoboken., 20, 19-19.
https://hdl.handle.net/21.15107/rcub_smile_1933

Matić-Petrović S, Milašin J, Vidaković M, Jotić A, Toljić B, Miličić B, Pucar A. TNFR2 polymorphism and serum concentrations in periodontitis and diabetes. in Oral Diseases. 2014;20:19-19.
https://hdl.handle.net/21.15107/rcub_smile_1933 .

Matić-Petrović, Sanja, Milašin, Jelena, Vidaković, M., Jotić, Aleksandra, Toljić, Boško, Miličić, Biljana, Pucar, Ana, "TNFR2 polymorphism and serum concentrations in periodontitis and diabetes" in Oral Diseases, 20 (2014):19-19,
https://hdl.handle.net/21.15107/rcub_smile_1933 .

TY  - CONF
AU  - Perić, M.
AU  - Pucar, Ana
AU  - Živković, Rade
AU  - Radunović, Milena
AU  - Pavlica, Dušan
AU  - Arsić-Arsenijević, Valentina
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1927
PB  - Wiley-Blackwell, Hoboken
C3  - Oral Diseases
T1  - Evaluation of different sampling methods in patients with denture stomatitis
VL  - 20
SP  - 22
EP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_smile_1927
ER  - 

@conference{
author = "Perić, M. and Pucar, Ana and Živković, Rade and Radunović, Milena and Pavlica, Dušan and Arsić-Arsenijević, Valentina",
year = "2014",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Oral Diseases",
title = "Evaluation of different sampling methods in patients with denture stomatitis",
volume = "20",
pages = "22-22",
url = "https://hdl.handle.net/21.15107/rcub_smile_1927"
}

Perić, M., Pucar, A., Živković, R., Radunović, M., Pavlica, D.,& Arsić-Arsenijević, V.. (2014). Evaluation of different sampling methods in patients with denture stomatitis. in Oral Diseases
Wiley-Blackwell, Hoboken., 20, 22-22.
https://hdl.handle.net/21.15107/rcub_smile_1927

Perić M, Pucar A, Živković R, Radunović M, Pavlica D, Arsić-Arsenijević V. Evaluation of different sampling methods in patients with denture stomatitis. in Oral Diseases. 2014;20:22-22.
https://hdl.handle.net/21.15107/rcub_smile_1927 .

Perić, M., Pucar, Ana, Živković, Rade, Radunović, Milena, Pavlica, Dušan, Arsić-Arsenijević, Valentina, "Evaluation of different sampling methods in patients with denture stomatitis" in Oral Diseases, 20 (2014):22-22,
https://hdl.handle.net/21.15107/rcub_smile_1927 .

TY  - CONF
AU  - Pucar, Ana
AU  - Matić-Petrović, Sanja
AU  - Radunović, Milena
AU  - Cimbaljević-Barac, M.
AU  - Kuzmanović-Pfićer, Jovana
AU  - Jotić, Aleksandra
AU  - Lalić, N.
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1928
PB  - Wiley-Blackwell, Hoboken
C3  - Oral Diseases
T1  - Presence and quantification of Candida spp in diabetics and nondiabetics
VL  - 20
SP  - 19
EP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_smile_1928
ER  - 

@conference{
author = "Pucar, Ana and Matić-Petrović, Sanja and Radunović, Milena and Cimbaljević-Barac, M. and Kuzmanović-Pfićer, Jovana and Jotić, Aleksandra and Lalić, N.",
year = "2014",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Oral Diseases",
title = "Presence and quantification of Candida spp in diabetics and nondiabetics",
volume = "20",
pages = "19-19",
url = "https://hdl.handle.net/21.15107/rcub_smile_1928"
}

Pucar, A., Matić-Petrović, S., Radunović, M., Cimbaljević-Barac, M., Kuzmanović-Pfićer, J., Jotić, A.,& Lalić, N.. (2014). Presence and quantification of Candida spp in diabetics and nondiabetics. in Oral Diseases
Wiley-Blackwell, Hoboken., 20, 19-19.
https://hdl.handle.net/21.15107/rcub_smile_1928

Pucar A, Matić-Petrović S, Radunović M, Cimbaljević-Barac M, Kuzmanović-Pfićer J, Jotić A, Lalić N. Presence and quantification of Candida spp in diabetics and nondiabetics. in Oral Diseases. 2014;20:19-19.
https://hdl.handle.net/21.15107/rcub_smile_1928 .

Pucar, Ana, Matić-Petrović, Sanja, Radunović, Milena, Cimbaljević-Barac, M., Kuzmanović-Pfićer, Jovana, Jotić, Aleksandra, Lalić, N., "Presence and quantification of Candida spp in diabetics and nondiabetics" in Oral Diseases, 20 (2014):19-19,
https://hdl.handle.net/21.15107/rcub_smile_1928 .

TY  - JOUR
AU  - Petrović-Matić, Sanja
AU  - Zelić, Ksenija
AU  - Milašin, Jelena
AU  - Popović, Branka
AU  - Pucar, Ana
AU  - Zelić, Obrad
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1917
AB  - Introduction Pathogenesis and some characteristics of periodontitis cannot be fully explained by bacterial etiology alone. Herpes viruses may bridge the gap between clinical characteristics and molecular understanding of periodontal destruction. Objective The aim of this study was to investigate the prevalence of herpes simplex virus type 1 (HSV-1) in gingival crevicular fluid (GCF) of healthy and damaged periodontium in Serbian population and to explore potential correlation between the presence of this virus and the level of periodontal destruction. Methods Samples were collected from gingival sulcus/periodontal pockets by sterile paper points and the presence of viral DNA in gingival crevicular fluid was assessed by PCR. Results There was no statistically significant difference in HSV-1 in presence between periodontitis patients (PG=38.9%) and healthy controls (HC=32.3%), (Chi-square test, with Yates' correction p=0.7574). However, HSV-1 positive patients showed significantly higher values of parameters of periodontal destruction (PPD=7.11±2.52, CAL=5.46±2.34) than periodontitis patients without HSV-1 in gingival crevicular fluid (PPD=4.70±1.79, CAL=3.39±2.65) (p values respectively, p=0.002 and p=0.023, Independent Samples T-Test). HSV-1 occurred more often in deeper (PPD≥6 mm) (69.2%) than in shallow pockets (3 mm lt PPD lt 6 mm) (18.2%) (Chi-square test, with Yates' correction, p=0.008). Plaque index was lower in the HSV-1 positive group (0.84± 0.69 vs. 1.43±0.76, p=0.023, Independent Samples T-Test). Conclusion This study demonstrated that the presence of HSV-1 in the gingival crevicular fluid coincides with a higher degree of tissue destruction in patients with periodontitis.
AB  - Uvod Patogeneza i neke kliničke odlike parodontopatije ne mogu se do kraja objasniti bakterijskom etiologijom. Herpesvirusi bi mogli da premoste jaz koji postoji između kliničkih osobina i poznavanja patogenetskih mehanizama parodontopatije na molekularnom nivou. Cilj rada Cilj ove studije bio je određivanje prevalencije virusa herpes simplex tip 1 (HSV-1) u zdravom i obolelom parodoncijumu osoba u Srbiji, kao i utvrđivanje moguće korelacije između postojanja ovih virusa i stepena oštećenja parodoncijuma. Metode rada Uzorci su uzimani papirnim poenima iz gingivalnih sulkusa ili parodontalnih džepova, a virus je otkrivan reakcijom lančanog umnožavanja molekula DNK (engl. polymerase chain reaction - PCR). Rezultati Nije utvrđena statistički značajna razlika u prevalenciji HSV-1 između osoba s parodontopatijom (32,3%) i zdravim parodoncijumom (38,9%); χ2-test sa Jejtsovom (Yates) korekcijom: p=0,7574. U grupi ispitanika s parodontopatijom parametri koji označavaju stepen oštećenja (dubina parodontalnog džepa - DPDž; nivo pripojnog epitela - NPE) bili su značajno veći kod onih s otkrivenim virusom (DPDž: 7,11±2,52 mm; NPE: 5,46±2,34), nego kod ispitanika bez virusa u uzorcima gingivalne tečnosti (DPDž=4,70±1,79 mm; NPE=3,39±2,65); Studentov t-test za nevezane uzorke: p=0,002, odnosno p=0,023. U dubljim parodontalnim džepovima (DPDž≥6 mm) HSV-1 je otkriven statistički značajno češće (69,2%) nego u plićim džepovima (DPDž=3-6 mm) (18,2%); χ2-test sa Jejtsovom korekcijom: p=0,008. Prosečne vrednosti plak-indeksa bile su niže kod ispitanika sa HSV-1 (0,84±0,69) u poređenju sa ispitanicima kod kojih ovaj virus nije otkriven (1,43±0,76); Studentov t-test za nevezane uzorke: p=0,023. Zaključak Prikazana studija pokazala je da je postojanje HSV-1 povezano sa nivoom oštećenja tkiva kod osoba s parodontopatijom.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction
T1  - Otkrivanje virusa herpes simplex tip 1 u gingivalnoj tečnosti sulkusa ili parodontalnog džepa lančanom reakcijom polimeraze
VL  - 142
IS  - 5-6
SP  - 296
EP  - 300
DO  - 10.2298/SARH1406296M
ER  - 

@article{
author = "Petrović-Matić, Sanja and Zelić, Ksenija and Milašin, Jelena and Popović, Branka and Pucar, Ana and Zelić, Obrad",
year = "2014",
abstract = "Introduction Pathogenesis and some characteristics of periodontitis cannot be fully explained by bacterial etiology alone. Herpes viruses may bridge the gap between clinical characteristics and molecular understanding of periodontal destruction. Objective The aim of this study was to investigate the prevalence of herpes simplex virus type 1 (HSV-1) in gingival crevicular fluid (GCF) of healthy and damaged periodontium in Serbian population and to explore potential correlation between the presence of this virus and the level of periodontal destruction. Methods Samples were collected from gingival sulcus/periodontal pockets by sterile paper points and the presence of viral DNA in gingival crevicular fluid was assessed by PCR. Results There was no statistically significant difference in HSV-1 in presence between periodontitis patients (PG=38.9%) and healthy controls (HC=32.3%), (Chi-square test, with Yates' correction p=0.7574). However, HSV-1 positive patients showed significantly higher values of parameters of periodontal destruction (PPD=7.11±2.52, CAL=5.46±2.34) than periodontitis patients without HSV-1 in gingival crevicular fluid (PPD=4.70±1.79, CAL=3.39±2.65) (p values respectively, p=0.002 and p=0.023, Independent Samples T-Test). HSV-1 occurred more often in deeper (PPD≥6 mm) (69.2%) than in shallow pockets (3 mm lt PPD lt 6 mm) (18.2%) (Chi-square test, with Yates' correction, p=0.008). Plaque index was lower in the HSV-1 positive group (0.84± 0.69 vs. 1.43±0.76, p=0.023, Independent Samples T-Test). Conclusion This study demonstrated that the presence of HSV-1 in the gingival crevicular fluid coincides with a higher degree of tissue destruction in patients with periodontitis., Uvod Patogeneza i neke kliničke odlike parodontopatije ne mogu se do kraja objasniti bakterijskom etiologijom. Herpesvirusi bi mogli da premoste jaz koji postoji između kliničkih osobina i poznavanja patogenetskih mehanizama parodontopatije na molekularnom nivou. Cilj rada Cilj ove studije bio je određivanje prevalencije virusa herpes simplex tip 1 (HSV-1) u zdravom i obolelom parodoncijumu osoba u Srbiji, kao i utvrđivanje moguće korelacije između postojanja ovih virusa i stepena oštećenja parodoncijuma. Metode rada Uzorci su uzimani papirnim poenima iz gingivalnih sulkusa ili parodontalnih džepova, a virus je otkrivan reakcijom lančanog umnožavanja molekula DNK (engl. polymerase chain reaction - PCR). Rezultati Nije utvrđena statistički značajna razlika u prevalenciji HSV-1 između osoba s parodontopatijom (32,3%) i zdravim parodoncijumom (38,9%); χ2-test sa Jejtsovom (Yates) korekcijom: p=0,7574. U grupi ispitanika s parodontopatijom parametri koji označavaju stepen oštećenja (dubina parodontalnog džepa - DPDž; nivo pripojnog epitela - NPE) bili su značajno veći kod onih s otkrivenim virusom (DPDž: 7,11±2,52 mm; NPE: 5,46±2,34), nego kod ispitanika bez virusa u uzorcima gingivalne tečnosti (DPDž=4,70±1,79 mm; NPE=3,39±2,65); Studentov t-test za nevezane uzorke: p=0,002, odnosno p=0,023. U dubljim parodontalnim džepovima (DPDž≥6 mm) HSV-1 je otkriven statistički značajno češće (69,2%) nego u plićim džepovima (DPDž=3-6 mm) (18,2%); χ2-test sa Jejtsovom korekcijom: p=0,008. Prosečne vrednosti plak-indeksa bile su niže kod ispitanika sa HSV-1 (0,84±0,69) u poređenju sa ispitanicima kod kojih ovaj virus nije otkriven (1,43±0,76); Studentov t-test za nevezane uzorke: p=0,023. Zaključak Prikazana studija pokazala je da je postojanje HSV-1 povezano sa nivoom oštećenja tkiva kod osoba s parodontopatijom.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction, Otkrivanje virusa herpes simplex tip 1 u gingivalnoj tečnosti sulkusa ili parodontalnog džepa lančanom reakcijom polimeraze",
volume = "142",
number = "5-6",
pages = "296-300",
doi = "10.2298/SARH1406296M"
}

Petrović-Matić, S., Zelić, K., Milašin, J., Popović, B., Pucar, A.,& Zelić, O.. (2014). Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 142(5-6), 296-300.
https://doi.org/10.2298/SARH1406296M

Petrović-Matić S, Zelić K, Milašin J, Popović B, Pucar A, Zelić O. Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction. in Srpski arhiv za celokupno lekarstvo. 2014;142(5-6):296-300.
doi:10.2298/SARH1406296M .

Petrović-Matić, Sanja, Zelić, Ksenija, Milašin, Jelena, Popović, Branka, Pucar, Ana, Zelić, Obrad, "Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction" in Srpski arhiv za celokupno lekarstvo, 142, no. 5-6 (2014):296-300,
https://doi.org/10.2298/SARH1406296M . .

TY  - JOUR
AU  - Shin, Ki-Hyuk
AU  - Kim, Reuben H.
AU  - Yu, Bo
AU  - Kang, Mo K.
AU  - Elashoff, David
AU  - Christensen, Russell
AU  - Pucar, Ana
AU  - Park, No-Hee
PY  - 2011
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1607
AB  - We previously reported that wild type (wt) hnRNP G exhibited tumor suppressive activity in human oral squamous cell carcinoma (HOSCC) cell lines lacking hnRNP G. Wt hnRNP G markedly inhibited the proliferation capacity, anchorage independency and in vivo tumorigenicity of HOSCC cells and notably enhanced the DNA repair capabilities of these cells. In the present study, we studied the genetic and expression states of hnRNP G in normal, premalignant and malignant human oral tissues to further understand the relationship between the hnRNP G alterations and the development of human oral cancer. To correlate the cancer development and the level of hnRNP G expression, we performed an immunohistochemistry staining of hnRNP G in normal, premalignant and malignant human oral tissues. Moreover, we examined the entire coding regions of hnRNP G from selected samples to understand the cause of the alterations of the gene expression. The expression of hnRNP G was notably decreased or completely abolished in 80% of premalignant-dysplastic and malignant oral epithelial tissues, whereas 100% of normal and 90% of hyperplastic non-dysplastic epithelium showed high level of hnRNP G in the nucleus of the basal cell layers. Approximately 80% of HOSCC lacking the expression of hnRNP G showed genetic alteration in hnRNP G, i.e., point mutation and exonic deletion. This study suggest that genetic alterations and aberrant expression of hnRNP G occurring during oral carcinogenesis might be useful markers for the early detection of human oral cancer. Published by Elsevier Ltd.
PB  - Elsevier Science Bv, Amsterdam
T2  - Oral Oncology
T1  - Expression and mutation analysis of heterogeneous nuclear ribonucleoprotein G in human oral cancer
VL  - 47
IS  - 11
SP  - 1011
EP  - 1016
DO  - 10.1016/j.oraloncology.2011.07.012
ER  - 

@article{
author = "Shin, Ki-Hyuk and Kim, Reuben H. and Yu, Bo and Kang, Mo K. and Elashoff, David and Christensen, Russell and Pucar, Ana and Park, No-Hee",
year = "2011",
abstract = "We previously reported that wild type (wt) hnRNP G exhibited tumor suppressive activity in human oral squamous cell carcinoma (HOSCC) cell lines lacking hnRNP G. Wt hnRNP G markedly inhibited the proliferation capacity, anchorage independency and in vivo tumorigenicity of HOSCC cells and notably enhanced the DNA repair capabilities of these cells. In the present study, we studied the genetic and expression states of hnRNP G in normal, premalignant and malignant human oral tissues to further understand the relationship between the hnRNP G alterations and the development of human oral cancer. To correlate the cancer development and the level of hnRNP G expression, we performed an immunohistochemistry staining of hnRNP G in normal, premalignant and malignant human oral tissues. Moreover, we examined the entire coding regions of hnRNP G from selected samples to understand the cause of the alterations of the gene expression. The expression of hnRNP G was notably decreased or completely abolished in 80% of premalignant-dysplastic and malignant oral epithelial tissues, whereas 100% of normal and 90% of hyperplastic non-dysplastic epithelium showed high level of hnRNP G in the nucleus of the basal cell layers. Approximately 80% of HOSCC lacking the expression of hnRNP G showed genetic alteration in hnRNP G, i.e., point mutation and exonic deletion. This study suggest that genetic alterations and aberrant expression of hnRNP G occurring during oral carcinogenesis might be useful markers for the early detection of human oral cancer. Published by Elsevier Ltd.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Oral Oncology",
title = "Expression and mutation analysis of heterogeneous nuclear ribonucleoprotein G in human oral cancer",
volume = "47",
number = "11",
pages = "1011-1016",
doi = "10.1016/j.oraloncology.2011.07.012"
}

Shin, K., Kim, R. H., Yu, B., Kang, M. K., Elashoff, D., Christensen, R., Pucar, A.,& Park, N.. (2011). Expression and mutation analysis of heterogeneous nuclear ribonucleoprotein G in human oral cancer. in Oral Oncology
Elsevier Science Bv, Amsterdam., 47(11), 1011-1016.
https://doi.org/10.1016/j.oraloncology.2011.07.012

Shin K, Kim RH, Yu B, Kang MK, Elashoff D, Christensen R, Pucar A, Park N. Expression and mutation analysis of heterogeneous nuclear ribonucleoprotein G in human oral cancer. in Oral Oncology. 2011;47(11):1011-1016.
doi:10.1016/j.oraloncology.2011.07.012 .

Shin, Ki-Hyuk, Kim, Reuben H., Yu, Bo, Kang, Mo K., Elashoff, David, Christensen, Russell, Pucar, Ana, Park, No-Hee, "Expression and mutation analysis of heterogeneous nuclear ribonucleoprotein G in human oral cancer" in Oral Oncology, 47, no. 11 (2011):1011-1016,
https://doi.org/10.1016/j.oraloncology.2011.07.012 . .

TY  - JOUR
AU  - Janković, Saša
AU  - Aleksić, Zoran
AU  - Nikolić-Jakoba, Nataša
AU  - Stanimirović, Dragan
AU  - Stojić, Ž.
AU  - Pucar, Ana
AU  - Hadži-Mihailović, Miloš
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1585
AB  - Periodontal disease is a chronic, multi-factorial disease of the tissues supporting the teeth. Periodontitis in companion animals is an almost identical disease to that in humans in terms of disease course and clinical presentation. Receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG) are bioactive molecules that control bone resorption. This study aims to evaluate the effect of Chlorhexidine (CXD) on the RANKL and OPG expressions in gingival crevicular fluid (GCF) collected from subjects with chronic periodontitis. GCF was obtained from subjects with chronic periodontitis.10 subjects (CXD1) rinsed the mouth with 0.12% CXD, 10 subjects (CXD2) utilized 0.20% CXD and the last 10 (PL) used Placebo solution for 7 days. RANKL and OPG concentrations in GCF were measured by enzyme-linked immunosorbent assays ELISA at baseline and after 7 days. Periodontal clinical variables: clinical attachment loss (CAL), probing pocket depth (PPD), papilla-bleeding index (PBI) were evaluated in all groups. After 7 days in CXD1 and CXD2 group RANKL/OPG ratio exhibited a significant decrease (p lt 0.05) in contrast to the PL group where results showed similar values of RANKL/OPG ratio at baseline and after the observation period. RANKL/OPG ratio was positively correlated with PPD, CAL and PBI before and after the observation period in both Chlorhexidine (CXD1, CXD2) groups. In an existing inflammatory response, chlorhexidine reduced the level of periodontal inflammation, which leads to reduction of RANKL/OPG relative ratio. Decrease of RANKL/OPG ratio will apparently induce maintenance of alveolar bone and slow down periodontal tissue breakdown.
AB  - Parodontopatije su hronična, multikauzalna oboljenja potpornog aparata zuba. Parodontalna oboljenja koja srećemo kod kućnih ljubimaca su prema toku i kliničkoj slici skoro identična onima koje se javljaju kod ljudi. RANKL i osteoprotegerin (OPG) su bioaktivni molekuli koji kontrolišu koštanu resorpciju. Cilj ove studije je evaluacija efekata hlorheksidina na ekspresiju RANKL-a i OPG-a u gingivalnoj tečnosti (GT) uzetoj od pacijenata sa hroničnom parodontopatijom. 10 pacijenata (CXD1) su ispirali usta sa 0.12% CXD, 10 pacijenata (CXD2) su koristili 0.20% CXD i poslednjih 10 pacijenata (PL) su koristili placebo rastvor 7 dana. RANKL i OPG koncentracije u GT su merene ELISA testom na početku i posle sedam dana. Parodontalni klinički parametri CAL, PPD i PBI su evaluirani u svim grupama. Posle 7 dana u CXD1 i CXD2 grupi RANKL/OPG odnos je pokazao signifikantno smanjenje (p lt 0.05) u poređenju sa PL grupom gde su zabaleženi slični rezultati na početku i nakon opservacionog perioda. RANKL/OPG odnos je pokazao pozitivnu korelaciju sa vrednostima PPD-a, CAL-a i PBI-a pre i nakon observacionog perioda u obe eksperimentalne grupe (CXD1, CXD2). U prisutnom inflamatornom odgovoru hlorheksidin je redukovao nivo inflamacije, što je uslovilo redukciju RANKL/OPG odnosa. Rezultati istraživanja dokazuju da koncentracija hlorheksidina ne utiče statistički značajno na smanjenje RANKL/OPG odnosa.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - The effect of chlorhexidine on the receptor activator of NF-kb ligand (RANKL) and osteoprotegerin (OPG) expression in chronic periodontitis in humans and companion animals
T1  - Efekat hlorheksidina na ekspresiju receptor aktivatora NF-kb liganda (RANKL) i osteoprotežerina (OPG) kod ljudi i kućnih ljubimaca
VL  - 60
IS  - 5-6
SP  - 641
EP  - 652
DO  - 10.2298/AVB1006641J
ER  - 

@article{
author = "Janković, Saša and Aleksić, Zoran and Nikolić-Jakoba, Nataša and Stanimirović, Dragan and Stojić, Ž. and Pucar, Ana and Hadži-Mihailović, Miloš",
year = "2010",
abstract = "Periodontal disease is a chronic, multi-factorial disease of the tissues supporting the teeth. Periodontitis in companion animals is an almost identical disease to that in humans in terms of disease course and clinical presentation. Receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG) are bioactive molecules that control bone resorption. This study aims to evaluate the effect of Chlorhexidine (CXD) on the RANKL and OPG expressions in gingival crevicular fluid (GCF) collected from subjects with chronic periodontitis. GCF was obtained from subjects with chronic periodontitis.10 subjects (CXD1) rinsed the mouth with 0.12% CXD, 10 subjects (CXD2) utilized 0.20% CXD and the last 10 (PL) used Placebo solution for 7 days. RANKL and OPG concentrations in GCF were measured by enzyme-linked immunosorbent assays ELISA at baseline and after 7 days. Periodontal clinical variables: clinical attachment loss (CAL), probing pocket depth (PPD), papilla-bleeding index (PBI) were evaluated in all groups. After 7 days in CXD1 and CXD2 group RANKL/OPG ratio exhibited a significant decrease (p lt 0.05) in contrast to the PL group where results showed similar values of RANKL/OPG ratio at baseline and after the observation period. RANKL/OPG ratio was positively correlated with PPD, CAL and PBI before and after the observation period in both Chlorhexidine (CXD1, CXD2) groups. In an existing inflammatory response, chlorhexidine reduced the level of periodontal inflammation, which leads to reduction of RANKL/OPG relative ratio. Decrease of RANKL/OPG ratio will apparently induce maintenance of alveolar bone and slow down periodontal tissue breakdown., Parodontopatije su hronična, multikauzalna oboljenja potpornog aparata zuba. Parodontalna oboljenja koja srećemo kod kućnih ljubimaca su prema toku i kliničkoj slici skoro identična onima koje se javljaju kod ljudi. RANKL i osteoprotegerin (OPG) su bioaktivni molekuli koji kontrolišu koštanu resorpciju. Cilj ove studije je evaluacija efekata hlorheksidina na ekspresiju RANKL-a i OPG-a u gingivalnoj tečnosti (GT) uzetoj od pacijenata sa hroničnom parodontopatijom. 10 pacijenata (CXD1) su ispirali usta sa 0.12% CXD, 10 pacijenata (CXD2) su koristili 0.20% CXD i poslednjih 10 pacijenata (PL) su koristili placebo rastvor 7 dana. RANKL i OPG koncentracije u GT su merene ELISA testom na početku i posle sedam dana. Parodontalni klinički parametri CAL, PPD i PBI su evaluirani u svim grupama. Posle 7 dana u CXD1 i CXD2 grupi RANKL/OPG odnos je pokazao signifikantno smanjenje (p lt 0.05) u poređenju sa PL grupom gde su zabaleženi slični rezultati na početku i nakon opservacionog perioda. RANKL/OPG odnos je pokazao pozitivnu korelaciju sa vrednostima PPD-a, CAL-a i PBI-a pre i nakon observacionog perioda u obe eksperimentalne grupe (CXD1, CXD2). U prisutnom inflamatornom odgovoru hlorheksidin je redukovao nivo inflamacije, što je uslovilo redukciju RANKL/OPG odnosa. Rezultati istraživanja dokazuju da koncentracija hlorheksidina ne utiče statistički značajno na smanjenje RANKL/OPG odnosa.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "The effect of chlorhexidine on the receptor activator of NF-kb ligand (RANKL) and osteoprotegerin (OPG) expression in chronic periodontitis in humans and companion animals, Efekat hlorheksidina na ekspresiju receptor aktivatora NF-kb liganda (RANKL) i osteoprotežerina (OPG) kod ljudi i kućnih ljubimaca",
volume = "60",
number = "5-6",
pages = "641-652",
doi = "10.2298/AVB1006641J"
}

Janković, S., Aleksić, Z., Nikolić-Jakoba, N., Stanimirović, D., Stojić, Ž., Pucar, A.,& Hadži-Mihailović, M.. (2010). The effect of chlorhexidine on the receptor activator of NF-kb ligand (RANKL) and osteoprotegerin (OPG) expression in chronic periodontitis in humans and companion animals. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(5-6), 641-652.
https://doi.org/10.2298/AVB1006641J

Janković S, Aleksić Z, Nikolić-Jakoba N, Stanimirović D, Stojić Ž, Pucar A, Hadži-Mihailović M. The effect of chlorhexidine on the receptor activator of NF-kb ligand (RANKL) and osteoprotegerin (OPG) expression in chronic periodontitis in humans and companion animals. in Acta veterinaria. 2010;60(5-6):641-652.
doi:10.2298/AVB1006641J .

Janković, Saša, Aleksić, Zoran, Nikolić-Jakoba, Nataša, Stanimirović, Dragan, Stojić, Ž., Pucar, Ana, Hadži-Mihailović, Miloš, "The effect of chlorhexidine on the receptor activator of NF-kb ligand (RANKL) and osteoprotegerin (OPG) expression in chronic periodontitis in humans and companion animals" in Acta veterinaria, 60, no. 5-6 (2010):641-652,
https://doi.org/10.2298/AVB1006641J . .

TY  - JOUR
AU  - Aleksić, Zoran
AU  - Janković, Saša
AU  - Dimitrijević, Božidar
AU  - Pucar, Ana
AU  - Lazić, Vojkan
AU  - Leković, Vojislav
PY  - 2008
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1437
AB  - Introduction Root coverage supported with complete regeneration of lost periodontal tissues represents the ultimate goal of gingival recession treatment. Objective This study was designed to evaluate clinical effectiveness of platelet rich plasma gel (PRP) with connective tissue graft (CTG) in the treatment of gingival recession. METHOD 15 gingival recessions Miller class I or II were treated with CTG and PRP (group PRP). Connective tissue graft was harvested from the premolar region using trap door technique. After elevation of the flap, the regional bone and root surface were smeared with activated PRP gel. CTG was also irrigated with PRP gel before placement over the exposed root surface and local bone. Fixed CTG was covered with a coronally advanced flap. The same number of gingival recessions were treated with CTG in combination with the coronally advanced flap with no PRP gel (group TVT). Clinical recordings included recession depth (RD), probing depth (PD), clinical attachment level (CAL) and keratinized tissue width (KT) before and 1 year after mucogingival surgical treatment. Results Mean value of RD was significantly decreased from 4.93±0.86 mm to 0.60±0.37 (p lt 0.01) with CTG and PRP and from 4.76±0.74 mm to 0.63±0.29 mm (p lt 0.01) in CTG group. This difference was not statistically significant. Results of the keratinized tissue width showed significant increase from 0.88± 0.30 mm presurgery to 3.78±0.49 mm (p lt 0.01) six months after treatment in PRP group and from 0.90±0.34 mm to 3.15±0.41 in TVT group (p lt 0.01). This difference was statistically significant (p>0.05). No statistically significant differences were observed between treatment groups in CAL and PD. Conclusion Clinical results validate both procedures as effective and highly predictable surgical techniques in solving gingival recession problem. Histological evaluation may confirm advantage of PRP use related to regeneration of periodontal tissues.
AB  - Uvod. Recesija gingive je jedan od najsloženijih problema savremene stomatologije. Iznalaženje novih terapijskih metoda za rešavanje ovog problema je značajan segment istraživanja u parodontologiji. Cilj rada. Cilj rada je bio da se procene klinički efekti primene plazme bogate trombocitima (PBT) u kombinaciji sa transplantatom vezivnog tkiva (TVT) u lečenju recesije gingive. Metod rada. Istraživanje je obuhvatilo 15 bolesnika s obostranim recesijama gingive klase II i III po Mileru (Miller). Metodološki koncept se zasnivao na metodu podeljenih usta. U lečenju 15 recesija primenjen je TVT u kombinaciji sa koronarno pozicioniranim režnjem uz primenu aktiviranog koncentrata trombocita (PBT grupa). Isti broj recesija na suprotnoj strani vilice zbrinjavan je autotransplantatom vezivnog tkiva i periosta u kombinaciji sa koronarno pozicioniranim režnjem (TVT grupa). Za procenu stanja parodoncijuma i efikasnosti primenjenih hirurških postupaka korišćeni su sledeći klinički parametri: vertikalna dubina recesije (VDR), nivo pripojnog epitela i širina keratinizovane gingive. Nivo oralne higijene je utvrđivan plak-indeksom po Silnes- Lou (Silness-Löu), a stanje gingive gingivnim indeksom po Lou-Silnesu (Löu-Silness). Rezultati. Na osnovu analize srednje vrednost za dubinu recesije (VDR), ustanovljeno je statistički značajno smanjenje vrednosti ovog parametra šest meseci posle tretmana u PBT grupi (sa 4,93±0,86 mm na 0,60±0,37 mm; p lt 0,01). U TVT grupi, u kojoj je vrednost VDR pre lečenja bila 4,76±0,74 mm, posle šest meseci dobijena je vrednost od 0,63±0,29 mm (p lt 0,01). U PBT grupi je posle opservacionog perioda zabeleženo proširenje zone keratinizovanog tkiva za 2,90 mm, dok je u istom periodu vrednost ovog parametra u TVT grupi bila 2,25 mm. Poređenjem prosečne promene vrednosti kliničkih parametara u okviru obe eksperimentalne grupe, samo je kod promene širine keratinizovanog tkiva zabeležena statistička značajnost (p lt 0,05). Zaključak. Rezultati ove kliničke studije ukazuju na izuzetnu efikasnost oba primenjena postupka u lečenju recesije gingive.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Clinical impact of platelet rich plasma in treatment of gingival recessions
T1  - Primena plazme bogate trombocitima u rekonstruktivnoj mukogingivnoj hirurgiji
VL  - 136
IS  - 3-4
SP  - 95
EP  - 103
DO  - 10.2298/SARH0804095A
ER  - 

@article{
author = "Aleksić, Zoran and Janković, Saša and Dimitrijević, Božidar and Pucar, Ana and Lazić, Vojkan and Leković, Vojislav",
year = "2008",
abstract = "Introduction Root coverage supported with complete regeneration of lost periodontal tissues represents the ultimate goal of gingival recession treatment. Objective This study was designed to evaluate clinical effectiveness of platelet rich plasma gel (PRP) with connective tissue graft (CTG) in the treatment of gingival recession. METHOD 15 gingival recessions Miller class I or II were treated with CTG and PRP (group PRP). Connective tissue graft was harvested from the premolar region using trap door technique. After elevation of the flap, the regional bone and root surface were smeared with activated PRP gel. CTG was also irrigated with PRP gel before placement over the exposed root surface and local bone. Fixed CTG was covered with a coronally advanced flap. The same number of gingival recessions were treated with CTG in combination with the coronally advanced flap with no PRP gel (group TVT). Clinical recordings included recession depth (RD), probing depth (PD), clinical attachment level (CAL) and keratinized tissue width (KT) before and 1 year after mucogingival surgical treatment. Results Mean value of RD was significantly decreased from 4.93±0.86 mm to 0.60±0.37 (p lt 0.01) with CTG and PRP and from 4.76±0.74 mm to 0.63±0.29 mm (p lt 0.01) in CTG group. This difference was not statistically significant. Results of the keratinized tissue width showed significant increase from 0.88± 0.30 mm presurgery to 3.78±0.49 mm (p lt 0.01) six months after treatment in PRP group and from 0.90±0.34 mm to 3.15±0.41 in TVT group (p lt 0.01). This difference was statistically significant (p>0.05). No statistically significant differences were observed between treatment groups in CAL and PD. Conclusion Clinical results validate both procedures as effective and highly predictable surgical techniques in solving gingival recession problem. Histological evaluation may confirm advantage of PRP use related to regeneration of periodontal tissues., Uvod. Recesija gingive je jedan od najsloženijih problema savremene stomatologije. Iznalaženje novih terapijskih metoda za rešavanje ovog problema je značajan segment istraživanja u parodontologiji. Cilj rada. Cilj rada je bio da se procene klinički efekti primene plazme bogate trombocitima (PBT) u kombinaciji sa transplantatom vezivnog tkiva (TVT) u lečenju recesije gingive. Metod rada. Istraživanje je obuhvatilo 15 bolesnika s obostranim recesijama gingive klase II i III po Mileru (Miller). Metodološki koncept se zasnivao na metodu podeljenih usta. U lečenju 15 recesija primenjen je TVT u kombinaciji sa koronarno pozicioniranim režnjem uz primenu aktiviranog koncentrata trombocita (PBT grupa). Isti broj recesija na suprotnoj strani vilice zbrinjavan je autotransplantatom vezivnog tkiva i periosta u kombinaciji sa koronarno pozicioniranim režnjem (TVT grupa). Za procenu stanja parodoncijuma i efikasnosti primenjenih hirurških postupaka korišćeni su sledeći klinički parametri: vertikalna dubina recesije (VDR), nivo pripojnog epitela i širina keratinizovane gingive. Nivo oralne higijene je utvrđivan plak-indeksom po Silnes- Lou (Silness-Löu), a stanje gingive gingivnim indeksom po Lou-Silnesu (Löu-Silness). Rezultati. Na osnovu analize srednje vrednost za dubinu recesije (VDR), ustanovljeno je statistički značajno smanjenje vrednosti ovog parametra šest meseci posle tretmana u PBT grupi (sa 4,93±0,86 mm na 0,60±0,37 mm; p lt 0,01). U TVT grupi, u kojoj je vrednost VDR pre lečenja bila 4,76±0,74 mm, posle šest meseci dobijena je vrednost od 0,63±0,29 mm (p lt 0,01). U PBT grupi je posle opservacionog perioda zabeleženo proširenje zone keratinizovanog tkiva za 2,90 mm, dok je u istom periodu vrednost ovog parametra u TVT grupi bila 2,25 mm. Poređenjem prosečne promene vrednosti kliničkih parametara u okviru obe eksperimentalne grupe, samo je kod promene širine keratinizovanog tkiva zabeležena statistička značajnost (p lt 0,05). Zaključak. Rezultati ove kliničke studije ukazuju na izuzetnu efikasnost oba primenjena postupka u lečenju recesije gingive.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Clinical impact of platelet rich plasma in treatment of gingival recessions, Primena plazme bogate trombocitima u rekonstruktivnoj mukogingivnoj hirurgiji",
volume = "136",
number = "3-4",
pages = "95-103",
doi = "10.2298/SARH0804095A"
}

Aleksić, Z., Janković, S., Dimitrijević, B., Pucar, A., Lazić, V.,& Leković, V.. (2008). Clinical impact of platelet rich plasma in treatment of gingival recessions. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 136(3-4), 95-103.
https://doi.org/10.2298/SARH0804095A

Aleksić Z, Janković S, Dimitrijević B, Pucar A, Lazić V, Leković V. Clinical impact of platelet rich plasma in treatment of gingival recessions. in Srpski arhiv za celokupno lekarstvo. 2008;136(3-4):95-103.
doi:10.2298/SARH0804095A .

Aleksić, Zoran, Janković, Saša, Dimitrijević, Božidar, Pucar, Ana, Lazić, Vojkan, Leković, Vojislav, "Clinical impact of platelet rich plasma in treatment of gingival recessions" in Srpski arhiv za celokupno lekarstvo, 136, no. 3-4 (2008):95-103,
https://doi.org/10.2298/SARH0804095A . .

TY  - JOUR
AU  - Pucar, Ana
AU  - Milašin, Jelena
AU  - Leković, Vojislav
AU  - Vukadinović, Miroslav
AU  - Ristić, Miljko
AU  - Putnik, Svetozar
AU  - Kenney, Barrie
PY  - 2007
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1349
AB  - Chronic infections, such as periodontitis, have been associated with an increase for atherosclerosis and coronary artery disease. The aim of this study was to investigate biopsy samples of coronary and internal mammary arteries for the presence of putative pathogenic bacteria (Porphyromanas gingivalis, Actinobacillus actinomycetemcomitans, Prevotella intermedia, and Tannerella forsythensis), Chlamydia pneumoniae, and human cytomegalovirus (CMV). Methods: Patients with a diagnosis of coronary artery disease were included in the study. fifteen coronary arteries with atherosclerosis and 15 internal mammary arteries without clinically assessable atherosclerotic degeneration were investigated. Both groups of specimens were obtained during coronary artery bypass grafting surgery. In all cases, the coronary and mammary artery specimens were taken from the same patient. The detection of periodontal pathogens, C. pneumoniae, and CMV was done by polymerase chain reaction analysis. Results: Bacterial DNA was found in nine of 15 (60%) coronary artery biopsy samples: P. gingivalis in eight (53.33%), A. actinomycetemcomitans in four (26.67%), P. intermedia in five (33.33%), and T. forsythensis in two (13.33%) samples; CMV was detected in 10 (66.67%) samples, and c. pneumoniae was detected in five (33.33%) samples. Some of the samples contained more than one type of bacteria. Periodontal pathogens were not detected in internal mammary artery biopsies, whereas CMV was present in seven (46.67%) samples and C. pneumoniae was present in six (40%) samples. Conclusion: The absence of putative pathogenic bacteria in internal mammary arteries, which are known to be affected rarely by atherosclerotic changes, and their presence in a high percentage of atherosclerotic coronary arteries support the concept that periodontal organisms are associated with the development and progression of atherosclerosis.
PB  - Wiley, Hoboken
T2  - Journal of Periodontology
T1  - Correlation between atherosclerosis and periodontal putative pathogenic bacterial infections in coronary and internal mammary arteries
VL  - 78
IS  - 4
SP  - 677
EP  - 682
DO  - 10.1902/jop.2007.060062
ER  - 

@article{
author = "Pucar, Ana and Milašin, Jelena and Leković, Vojislav and Vukadinović, Miroslav and Ristić, Miljko and Putnik, Svetozar and Kenney, Barrie",
year = "2007",
abstract = "Chronic infections, such as periodontitis, have been associated with an increase for atherosclerosis and coronary artery disease. The aim of this study was to investigate biopsy samples of coronary and internal mammary arteries for the presence of putative pathogenic bacteria (Porphyromanas gingivalis, Actinobacillus actinomycetemcomitans, Prevotella intermedia, and Tannerella forsythensis), Chlamydia pneumoniae, and human cytomegalovirus (CMV). Methods: Patients with a diagnosis of coronary artery disease were included in the study. fifteen coronary arteries with atherosclerosis and 15 internal mammary arteries without clinically assessable atherosclerotic degeneration were investigated. Both groups of specimens were obtained during coronary artery bypass grafting surgery. In all cases, the coronary and mammary artery specimens were taken from the same patient. The detection of periodontal pathogens, C. pneumoniae, and CMV was done by polymerase chain reaction analysis. Results: Bacterial DNA was found in nine of 15 (60%) coronary artery biopsy samples: P. gingivalis in eight (53.33%), A. actinomycetemcomitans in four (26.67%), P. intermedia in five (33.33%), and T. forsythensis in two (13.33%) samples; CMV was detected in 10 (66.67%) samples, and c. pneumoniae was detected in five (33.33%) samples. Some of the samples contained more than one type of bacteria. Periodontal pathogens were not detected in internal mammary artery biopsies, whereas CMV was present in seven (46.67%) samples and C. pneumoniae was present in six (40%) samples. Conclusion: The absence of putative pathogenic bacteria in internal mammary arteries, which are known to be affected rarely by atherosclerotic changes, and their presence in a high percentage of atherosclerotic coronary arteries support the concept that periodontal organisms are associated with the development and progression of atherosclerosis.",
publisher = "Wiley, Hoboken",
journal = "Journal of Periodontology",
title = "Correlation between atherosclerosis and periodontal putative pathogenic bacterial infections in coronary and internal mammary arteries",
volume = "78",
number = "4",
pages = "677-682",
doi = "10.1902/jop.2007.060062"
}

Pucar, A., Milašin, J., Leković, V., Vukadinović, M., Ristić, M., Putnik, S.,& Kenney, B.. (2007). Correlation between atherosclerosis and periodontal putative pathogenic bacterial infections in coronary and internal mammary arteries. in Journal of Periodontology
Wiley, Hoboken., 78(4), 677-682.
https://doi.org/10.1902/jop.2007.060062

Pucar A, Milašin J, Leković V, Vukadinović M, Ristić M, Putnik S, Kenney B. Correlation between atherosclerosis and periodontal putative pathogenic bacterial infections in coronary and internal mammary arteries. in Journal of Periodontology. 2007;78(4):677-682.
doi:10.1902/jop.2007.060062 .

Pucar, Ana, Milašin, Jelena, Leković, Vojislav, Vukadinović, Miroslav, Ristić, Miljko, Putnik, Svetozar, Kenney, Barrie, "Correlation between atherosclerosis and periodontal putative pathogenic bacterial infections in coronary and internal mammary arteries" in Journal of Periodontology, 78, no. 4 (2007):677-682,
https://doi.org/10.1902/jop.2007.060062 . .

TY  - JOUR
AU  - Checchi, Luigi
AU  - Pucar, Ana
AU  - Checchi, Vittorio
PY  - 2007
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1354
AB  - Oral leukoplakia is a predominantly white lesion of the oral mucosa that cannot be clinico-pathologically characterized as any other definable lesion. Any oral site may be affected by leukoplakia, the most common sites being buccal and alveolar mucosa, floor of the mouth, tongue, lips, and palate. To date there is no evidence of effective treatment of oral leukoplakia that may prevent recurrence. This case report describes a new surgical technique using a bilaminar connective tissue graft in the treatment of oral leukoplakia. During the regular periodontal recall visit, the clinical diagnosis of gingival leukoplakia at the maxillary left sextant was established in a 45-year-old patient. Histopathologic analysis suggested reactive hyperkeratosis. The patient agreed to a new surgical treatment of the lesion. Under local anesthesia a 20-mm-long bilaminar connective tissue graft was interposed between the affected tissue and the bone. Healing was followed by the disappearance of the white lesion within the borders of the underlying graft. Five years after therapy, the treated area remained intact, with no clinical sign of recurrence.
PB  - Quintessence Publishing Co. Inc.
T2  - Quintessence International
T1  - Bilaminar connective tissue graft as an alternative treatment of leukoplakia: Case report
VL  - 38
IS  - 1
SP  - 39
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_smile_1354
ER  - 

@article{
author = "Checchi, Luigi and Pucar, Ana and Checchi, Vittorio",
year = "2007",
abstract = "Oral leukoplakia is a predominantly white lesion of the oral mucosa that cannot be clinico-pathologically characterized as any other definable lesion. Any oral site may be affected by leukoplakia, the most common sites being buccal and alveolar mucosa, floor of the mouth, tongue, lips, and palate. To date there is no evidence of effective treatment of oral leukoplakia that may prevent recurrence. This case report describes a new surgical technique using a bilaminar connective tissue graft in the treatment of oral leukoplakia. During the regular periodontal recall visit, the clinical diagnosis of gingival leukoplakia at the maxillary left sextant was established in a 45-year-old patient. Histopathologic analysis suggested reactive hyperkeratosis. The patient agreed to a new surgical treatment of the lesion. Under local anesthesia a 20-mm-long bilaminar connective tissue graft was interposed between the affected tissue and the bone. Healing was followed by the disappearance of the white lesion within the borders of the underlying graft. Five years after therapy, the treated area remained intact, with no clinical sign of recurrence.",
publisher = "Quintessence Publishing Co. Inc.",
journal = "Quintessence International",
title = "Bilaminar connective tissue graft as an alternative treatment of leukoplakia: Case report",
volume = "38",
number = "1",
pages = "39-44",
url = "https://hdl.handle.net/21.15107/rcub_smile_1354"
}

Checchi, L., Pucar, A.,& Checchi, V.. (2007). Bilaminar connective tissue graft as an alternative treatment of leukoplakia: Case report. in Quintessence International
Quintessence Publishing Co. Inc.., 38(1), 39-44.
https://hdl.handle.net/21.15107/rcub_smile_1354

Checchi L, Pucar A, Checchi V. Bilaminar connective tissue graft as an alternative treatment of leukoplakia: Case report. in Quintessence International. 2007;38(1):39-44.
https://hdl.handle.net/21.15107/rcub_smile_1354 .

Checchi, Luigi, Pucar, Ana, Checchi, Vittorio, "Bilaminar connective tissue graft as an alternative treatment of leukoplakia: Case report" in Quintessence International, 38, no. 1 (2007):39-44,
https://hdl.handle.net/21.15107/rcub_smile_1354 .

TY  - THES
AU  - Pucar, Ana
PY  - 2007
UR  - https://plus.sr.cobiss.net/opac7/bib/33201935
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/449
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - Ispitivanje prisustva mikroorganizama oralne mikroflore kao faktora rizika u nastanku i razvoju okluzivne arterijske bolesti
UR  - https://hdl.handle.net/21.15107/rcub_smile_449
ER  - 

@phdthesis{
author = "Pucar, Ana",
year = "2007",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "Ispitivanje prisustva mikroorganizama oralne mikroflore kao faktora rizika u nastanku i razvoju okluzivne arterijske bolesti",
url = "https://hdl.handle.net/21.15107/rcub_smile_449"
}

Pucar, A.. (2007). Ispitivanje prisustva mikroorganizama oralne mikroflore kao faktora rizika u nastanku i razvoju okluzivne arterijske bolesti. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_smile_449

Pucar A. Ispitivanje prisustva mikroorganizama oralne mikroflore kao faktora rizika u nastanku i razvoju okluzivne arterijske bolesti. 2007;.
https://hdl.handle.net/21.15107/rcub_smile_449 .

Pucar, Ana, "Ispitivanje prisustva mikroorganizama oralne mikroflore kao faktora rizika u nastanku i razvoju okluzivne arterijske bolesti" (2007),
https://hdl.handle.net/21.15107/rcub_smile_449 .

TY  - JOUR
AU  - Hadži-Mihailović, Miloš
AU  - Pucar, Ana
AU  - Janković, Ljiljana
PY  - 2003
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1173
AB  - Sjögren’s syndrome (SS) is a chronic autoimmune disorder with its major clinical manifestation resulting from changes in exocrine glands. An autoimmune etiology of SS is supported by the presence of autoantibodies specific for nuclear anti Ro (SS-A) and anti La (SS-B) antigens and other antinuclear antibodies (ANA) in sera of SS patients. The aim of the study was to evaluate the frequency of positive values of anti SS-A, anti SS-B antibodies and ANA in serum of 40 SS patients, and to correlate the above mentioned parameters with unstimulated salivary flow rate (USFR) and labial salivary gland focus score (LFS). Total of 40 patients was included in this research, 23 patients with primary SS and 17 with secondary SS. ANA was detected by the method of indirect immunofluorescence, and anti Ro/SS-A and anti La/SS-B antibodies were quantified by sandwich (Elisa) assay. The positive values of ANA were identified in plasma of 32 examined SS patients (80%), anti SS-A (Ro-52 kDa, Ro-60 kDa) antibodies were positive by 22 patients (55%), and anti SS-B antibodies were positive in less then 50% of examined SS patients (40%). The most presented were the patients with medium expressed xerostomia. Significant correlation between the unstimulated salivary flow rate and the positive values of ANA, anti Ro/SS-A, and anti La/SS-B antibodies could not be noticed. With increasing severity of disease there was an increase of the number of lymphocyte foci and a progressive loss of acinar tissue. High level of anti Ro/SS-A antibodies was found more often in plasma of the SS patients in a case of progressive loss of acinar tissue of the salivary gland (terminal stage), with statistical significance. It was concluded that the increased prevalence of positive values of anti Ro/SS-A antibodies and ANA indicates that the patient with SS has developed higher level of autoimmune reactivity. Level of anti Ro/SS-A antibodies in the peripheral blood of the patient with SS could be a significant marker of the degree of salivary/tissue destruction and disease severity.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Stomatology
T1  - Association of unstimulated salivary flow rate and labial salivary gland histopathology with serologic features of Sjögren’s syndrome
VL  - 7
IS  - 2
SP  - 82
EP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_smile_1173
ER  - 

@article{
author = "Hadži-Mihailović, Miloš and Pucar, Ana and Janković, Ljiljana",
year = "2003",
abstract = "Sjögren’s syndrome (SS) is a chronic autoimmune disorder with its major clinical manifestation resulting from changes in exocrine glands. An autoimmune etiology of SS is supported by the presence of autoantibodies specific for nuclear anti Ro (SS-A) and anti La (SS-B) antigens and other antinuclear antibodies (ANA) in sera of SS patients. The aim of the study was to evaluate the frequency of positive values of anti SS-A, anti SS-B antibodies and ANA in serum of 40 SS patients, and to correlate the above mentioned parameters with unstimulated salivary flow rate (USFR) and labial salivary gland focus score (LFS). Total of 40 patients was included in this research, 23 patients with primary SS and 17 with secondary SS. ANA was detected by the method of indirect immunofluorescence, and anti Ro/SS-A and anti La/SS-B antibodies were quantified by sandwich (Elisa) assay. The positive values of ANA were identified in plasma of 32 examined SS patients (80%), anti SS-A (Ro-52 kDa, Ro-60 kDa) antibodies were positive by 22 patients (55%), and anti SS-B antibodies were positive in less then 50% of examined SS patients (40%). The most presented were the patients with medium expressed xerostomia. Significant correlation between the unstimulated salivary flow rate and the positive values of ANA, anti Ro/SS-A, and anti La/SS-B antibodies could not be noticed. With increasing severity of disease there was an increase of the number of lymphocyte foci and a progressive loss of acinar tissue. High level of anti Ro/SS-A antibodies was found more often in plasma of the SS patients in a case of progressive loss of acinar tissue of the salivary gland (terminal stage), with statistical significance. It was concluded that the increased prevalence of positive values of anti Ro/SS-A antibodies and ANA indicates that the patient with SS has developed higher level of autoimmune reactivity. Level of anti Ro/SS-A antibodies in the peripheral blood of the patient with SS could be a significant marker of the degree of salivary/tissue destruction and disease severity.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Stomatology",
title = "Association of unstimulated salivary flow rate and labial salivary gland histopathology with serologic features of Sjögren’s syndrome",
volume = "7",
number = "2",
pages = "82-86",
url = "https://hdl.handle.net/21.15107/rcub_smile_1173"
}

Hadži-Mihailović, M., Pucar, A.,& Janković, L.. (2003). Association of unstimulated salivary flow rate and labial salivary gland histopathology with serologic features of Sjögren’s syndrome. in Balkan Journal of Stomatology
Udruženje stomatologa Balkana., 7(2), 82-86.
https://hdl.handle.net/21.15107/rcub_smile_1173

Hadži-Mihailović M, Pucar A, Janković L. Association of unstimulated salivary flow rate and labial salivary gland histopathology with serologic features of Sjögren’s syndrome. in Balkan Journal of Stomatology. 2003;7(2):82-86.
https://hdl.handle.net/21.15107/rcub_smile_1173 .

Hadži-Mihailović, Miloš, Pucar, Ana, Janković, Ljiljana, "Association of unstimulated salivary flow rate and labial salivary gland histopathology with serologic features of Sjögren’s syndrome" in Balkan Journal of Stomatology, 7, no. 2 (2003):82-86,
https://hdl.handle.net/21.15107/rcub_smile_1173 .

TY  - THES
AU  - Pucar, Ana
PY  - 2000
UR  - https://plus.sr.cobiss.net/opac7/bib/1024051086
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/359
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - Oralne manifestacije u toku hemioterapije kolorektalnog karcinoma
UR  - https://hdl.handle.net/21.15107/rcub_smile_359
ER  - 

@mastersthesis{
author = "Pucar, Ana",
year = "2000",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "Oralne manifestacije u toku hemioterapije kolorektalnog karcinoma",
url = "https://hdl.handle.net/21.15107/rcub_smile_359"
}

Pucar, A.. (2000). Oralne manifestacije u toku hemioterapije kolorektalnog karcinoma. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_smile_359

Pucar A. Oralne manifestacije u toku hemioterapije kolorektalnog karcinoma. 2000;.
https://hdl.handle.net/21.15107/rcub_smile_359 .

Pucar, Ana, "Oralne manifestacije u toku hemioterapije kolorektalnog karcinoma" (2000),
https://hdl.handle.net/21.15107/rcub_smile_359 .

TY  - CONF
AU  - Pucar, Ana
AU  - Marković, D.
PY  - 1998
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1089
PB  - Sage Publications Inc, Thousand Oaks
C3  - Journal of Dental Research
T1  - Investigation or bioceramic root sealer cytotoxicity on cell culture
VL  - 77
IS  - 5
SP  - 1249
EP  - 1249
UR  - https://hdl.handle.net/21.15107/rcub_smile_1089
ER  - 

@conference{
author = "Pucar, Ana and Marković, D.",
year = "1998",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Journal of Dental Research",
title = "Investigation or bioceramic root sealer cytotoxicity on cell culture",
volume = "77",
number = "5",
pages = "1249-1249",
url = "https://hdl.handle.net/21.15107/rcub_smile_1089"
}

Pucar, A.,& Marković, D.. (1998). Investigation or bioceramic root sealer cytotoxicity on cell culture. in Journal of Dental Research
Sage Publications Inc, Thousand Oaks., 77(5), 1249-1249.
https://hdl.handle.net/21.15107/rcub_smile_1089

Pucar A, Marković D. Investigation or bioceramic root sealer cytotoxicity on cell culture. in Journal of Dental Research. 1998;77(5):1249-1249.
https://hdl.handle.net/21.15107/rcub_smile_1089 .

Pucar, Ana, Marković, D., "Investigation or bioceramic root sealer cytotoxicity on cell culture" in Journal of Dental Research, 77, no. 5 (1998):1249-1249,
https://hdl.handle.net/21.15107/rcub_smile_1089 .