TY  - JOUR
AU  - Matić Petrović, Sanja
AU  - Nikolić, Nadja
AU  - Toljić, Boško
AU  - Arambašić-Jovanović, Jelena
AU  - Miličić, Biljana
AU  - Miličić, Tanja
AU  - Jotić, Aleksandra
AU  - Vidaković, Melita
AU  - Milašin, Jelena
AU  - Pucar, Ana
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2571
AB  - Objectives: Aiming to show that periodontitis (PD) and type 2 diabetes (T2D) are bidirectionally related and potentially linked by inflammatory cytokines, we searched for association between -308 G/A Tumor necrosis factor-alpha (TNFα), +252A/G Lymphotoxin-alpha (LTα), +36A/G Tumor necrosis factor receptor 1 (TNFR1) and +676 T/G tumor necrosis factor receptor 2 (TNFR2) single nucleotide polymorphisms (SNPs) and: risk of PD or PD + T2D; periodontitis parameters in PD and PD + T2D; serum levels of cytokines/their receptors. Relationship between periodontal inflammation and serum cytokine/receptor levels was also assessed.

Design: Subjects were stratified as: 57 healthy controls (HC); 58 PD; 65 PD + T2D. Sociodemographic, environmental, behavioral and periodontal clinical data were recorded. SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism, while cytokines/receptors levels were quantified by enzyme-linked immunosorbent assay. Impact of periodontal inflammation was measured using periodontal inflamed surface area (PISA).

Results: TNFα AA genotype showed protective effect in T2D + PD compared to PD, even adjusted for behavioral/environmental factors (OR 0.18; 95 %CI 0.037-0.886; p = 0.035). LTα AG heterozygotes had increased risk of PD (OR 3.27; 95 %CI 1.35-7.96; p = 0.016), while TNFR2 TG genotype had protective effect (OR = 0.44; 95 %CI 0.954-0.9794; p = 0.043). TNFR1 AA was predictor of periodontal pocket depth and clinical attachment loss in PD. Correlation between TNFR2 concentration and PISA was negative in PD, positive in PD + T2D.

Conclusions: None of the SNPs showed cross-susceptibility between PD and T2D. + 252A/G LTα and +676 T/G TNFR2 SNPs are associated with PD risk. Periodontal destruction in healthy individuals is influenced by TNFR1 genotype. Impact of periodontal on systemic inflammation is masked by T2D.
PB  - Elsevier Inc.
T2  - Archives of Oral Biology
T1  - The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population
VL  - 120
SP  - 104929
DO  - 10.1016/j.archoralbio.2020.104929
ER  - 

@article{
author = "Matić Petrović, Sanja and Nikolić, Nadja and Toljić, Boško and Arambašić-Jovanović, Jelena and Miličić, Biljana and Miličić, Tanja and Jotić, Aleksandra and Vidaković, Melita and Milašin, Jelena and Pucar, Ana",
year = "2020",
abstract = "Objectives: Aiming to show that periodontitis (PD) and type 2 diabetes (T2D) are bidirectionally related and potentially linked by inflammatory cytokines, we searched for association between -308 G/A Tumor necrosis factor-alpha (TNFα), +252A/G Lymphotoxin-alpha (LTα), +36A/G Tumor necrosis factor receptor 1 (TNFR1) and +676 T/G tumor necrosis factor receptor 2 (TNFR2) single nucleotide polymorphisms (SNPs) and: risk of PD or PD + T2D; periodontitis parameters in PD and PD + T2D; serum levels of cytokines/their receptors. Relationship between periodontal inflammation and serum cytokine/receptor levels was also assessed.

Design: Subjects were stratified as: 57 healthy controls (HC); 58 PD; 65 PD + T2D. Sociodemographic, environmental, behavioral and periodontal clinical data were recorded. SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism, while cytokines/receptors levels were quantified by enzyme-linked immunosorbent assay. Impact of periodontal inflammation was measured using periodontal inflamed surface area (PISA).

Results: TNFα AA genotype showed protective effect in T2D + PD compared to PD, even adjusted for behavioral/environmental factors (OR 0.18; 95 %CI 0.037-0.886; p = 0.035). LTα AG heterozygotes had increased risk of PD (OR 3.27; 95 %CI 1.35-7.96; p = 0.016), while TNFR2 TG genotype had protective effect (OR = 0.44; 95 %CI 0.954-0.9794; p = 0.043). TNFR1 AA was predictor of periodontal pocket depth and clinical attachment loss in PD. Correlation between TNFR2 concentration and PISA was negative in PD, positive in PD + T2D.

Conclusions: None of the SNPs showed cross-susceptibility between PD and T2D. + 252A/G LTα and +676 T/G TNFR2 SNPs are associated with PD risk. Periodontal destruction in healthy individuals is influenced by TNFR1 genotype. Impact of periodontal on systemic inflammation is masked by T2D.",
publisher = "Elsevier Inc.",
journal = "Archives of Oral Biology",
title = "The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population",
volume = "120",
pages = "104929",
doi = "10.1016/j.archoralbio.2020.104929"
}

Matić Petrović, S., Nikolić, N., Toljić, B., Arambašić-Jovanović, J., Miličić, B., Miličić, T., Jotić, A., Vidaković, M., Milašin, J.,& Pucar, A.. (2020). The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population. in Archives of Oral Biology
Elsevier Inc.., 120, 104929.
https://doi.org/10.1016/j.archoralbio.2020.104929

Matić Petrović S, Nikolić N, Toljić B, Arambašić-Jovanović J, Miličić B, Miličić T, Jotić A, Vidaković M, Milašin J, Pucar A. The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population. in Archives of Oral Biology. 2020;120:104929.
doi:10.1016/j.archoralbio.2020.104929 .

Matić Petrović, Sanja, Nikolić, Nadja, Toljić, Boško, Arambašić-Jovanović, Jelena, Miličić, Biljana, Miličić, Tanja, Jotić, Aleksandra, Vidaković, Melita, Milašin, Jelena, Pucar, Ana, "The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population" in Archives of Oral Biology, 120 (2020):104929,
https://doi.org/10.1016/j.archoralbio.2020.104929 . .

TY  - JOUR
AU  - Matić-Petrović, Sanja
AU  - Radunović, Milena
AU  - Barać, Milena
AU  - Kuzmanović-Pfićer, Jovana
AU  - Pavlica, Dušan
AU  - Arsić-Arsenijević, Valentina
AU  - Pucar, Ana
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2463
AB  - Objectives The aim of this cross-sectional observational study was to compare the prevalence of different oral Candida spp. in patients with Type 2 Diabetes and chronic periodontitis in two oral sites: dorsal surface of the tongue and subgingival area. In order to determine subgingival areas as potential reservoirs of yeasts, this study aimed to find differences in the yeasts' detection between the dorsum of the tongue, as the oral site most commonly inhabited with microorganisms, and subgingival samples. Additionally, potential predictors for the yeasts prevalence were determined. Material and methods Subjects (N = 146) were divided into four groups: group A-healthy individuals without periodontitis, group B-healthy individuals with chronic periodontitis, group C-Type 2 Diabetes patients with good glycoregulation and Chronic periodontitis and group D-Type 2 Diabetes patients with poor glycoregulation and Chronic periodontitis. Samples were obtained from the tongue by swabbing. Subgingival plaque samples were taken by paper points and periodontal curette. Isolation and identification of different Candida spp. was done using ChromAgar medium. In addition, germ-tube production and carbohydrate assimilation tests were performed. Results The prevalence of Candida spp. was higher in diabetics with poor glycoregulation. The most frequently isolated species was Candida albicans followed by Candida glabrata and Candida tropicalis. In 15.6% of cases, Candida spp. was present in the subgingival area while absent on the tongue. Multivariate regression model showed that HbA1c was Candida spp. predictor for both locations. Conclusions Our results confirmed that there are Candida spp. carriers among subjects with clinically healthy oral mucosa. Also, this study identified subgingival areas as potential reservoirs of these pathogenic species. Glycoregulation has been recognized as a positive predictor factor of Candida spp.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects
VL  - 14
IS  - 1
DO  - 10.1371/journal.pone.0210527
ER  - 

@article{
author = "Matić-Petrović, Sanja and Radunović, Milena and Barać, Milena and Kuzmanović-Pfićer, Jovana and Pavlica, Dušan and Arsić-Arsenijević, Valentina and Pucar, Ana",
year = "2019",
abstract = "Objectives The aim of this cross-sectional observational study was to compare the prevalence of different oral Candida spp. in patients with Type 2 Diabetes and chronic periodontitis in two oral sites: dorsal surface of the tongue and subgingival area. In order to determine subgingival areas as potential reservoirs of yeasts, this study aimed to find differences in the yeasts' detection between the dorsum of the tongue, as the oral site most commonly inhabited with microorganisms, and subgingival samples. Additionally, potential predictors for the yeasts prevalence were determined. Material and methods Subjects (N = 146) were divided into four groups: group A-healthy individuals without periodontitis, group B-healthy individuals with chronic periodontitis, group C-Type 2 Diabetes patients with good glycoregulation and Chronic periodontitis and group D-Type 2 Diabetes patients with poor glycoregulation and Chronic periodontitis. Samples were obtained from the tongue by swabbing. Subgingival plaque samples were taken by paper points and periodontal curette. Isolation and identification of different Candida spp. was done using ChromAgar medium. In addition, germ-tube production and carbohydrate assimilation tests were performed. Results The prevalence of Candida spp. was higher in diabetics with poor glycoregulation. The most frequently isolated species was Candida albicans followed by Candida glabrata and Candida tropicalis. In 15.6% of cases, Candida spp. was present in the subgingival area while absent on the tongue. Multivariate regression model showed that HbA1c was Candida spp. predictor for both locations. Conclusions Our results confirmed that there are Candida spp. carriers among subjects with clinically healthy oral mucosa. Also, this study identified subgingival areas as potential reservoirs of these pathogenic species. Glycoregulation has been recognized as a positive predictor factor of Candida spp.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects",
volume = "14",
number = "1",
doi = "10.1371/journal.pone.0210527"
}

Matić-Petrović, S., Radunović, M., Barać, M., Kuzmanović-Pfićer, J., Pavlica, D., Arsić-Arsenijević, V.,& Pucar, A.. (2019). Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects. in PLoS One
Public Library Science, San Francisco., 14(1).
https://doi.org/10.1371/journal.pone.0210527

Matić-Petrović S, Radunović M, Barać M, Kuzmanović-Pfićer J, Pavlica D, Arsić-Arsenijević V, Pucar A. Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects. in PLoS One. 2019;14(1).
doi:10.1371/journal.pone.0210527 .

Matić-Petrović, Sanja, Radunović, Milena, Barać, Milena, Kuzmanović-Pfićer, Jovana, Pavlica, Dušan, Arsić-Arsenijević, Valentina, Pucar, Ana, "Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects" in PLoS One, 14, no. 1 (2019),
https://doi.org/10.1371/journal.pone.0210527 . .

TY  - JOUR
AU  - Matić-Petrović, Sanja
AU  - Đorđević, Mihajlo
AU  - Radunović, Milena
AU  - Živanović, Tanja
AU  - Pavlica, Dušan
AU  - Pucar, Ana
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2406
AB  - Background/Aim: Geographic tongue (GT), a benign self-limiting condition (inflammation) is commonly seen in practice. Although quite easy for clinical diagnosis, in clinical practice it is commonly misdiagnosed and treated as Candida infection. The main aim of this study was to compare the prevalence of Candida spp. on tongue in patients with GT and subjects with clinically healthy oral mucosa. Additional aims were to evaluate subjective symptoms, uncontrolled usage of prescribed or non-prescribed antifungal topical medications and the presence of the cancerophobia or other health concerns in patients with GT. Material and Methods: A total of 70 subjects were divided into two groups: Group B - patients with diagnosed Geographic tongue and group A - aged and gender matched controls with clinically health oral mucosa. Anamnestic charts designed for this study included information about symptoms (measured by Visual Analogue Scale), previous knowledge or fear about presence of GT and received therapy for this condition. Detection of Candida spp. from tongue was done using sterile cotton swab and Sabouraud dextrose agar. Results: At the time of this study, 18 (52.94%) of subjects with GT were aware of having this condition and even 12 of them (66.7%) used topical antifungal drugs prescribed by physician. Also, 66.7% of them experienced fear about the presence of GT at some moment. Detection of Candida spp. was similarly distributed in both groups (22.2% in group A and 17.6% in group B). Conclusions: Geographic tongue has frequently been treated by topical antifungal drugs. In this study, GT was not associated with presence of Candida spp.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Dental Medicine
T1  - Geographic tongue: Does Candida play a role in its pathogenesis
VL  - 23
IS  - 3
SP  - 152
EP  - 156
DO  - 10.2478/bjdm-2019-0027
ER  - 

@article{
author = "Matić-Petrović, Sanja and Đorđević, Mihajlo and Radunović, Milena and Živanović, Tanja and Pavlica, Dušan and Pucar, Ana",
year = "2019",
abstract = "Background/Aim: Geographic tongue (GT), a benign self-limiting condition (inflammation) is commonly seen in practice. Although quite easy for clinical diagnosis, in clinical practice it is commonly misdiagnosed and treated as Candida infection. The main aim of this study was to compare the prevalence of Candida spp. on tongue in patients with GT and subjects with clinically healthy oral mucosa. Additional aims were to evaluate subjective symptoms, uncontrolled usage of prescribed or non-prescribed antifungal topical medications and the presence of the cancerophobia or other health concerns in patients with GT. Material and Methods: A total of 70 subjects were divided into two groups: Group B - patients with diagnosed Geographic tongue and group A - aged and gender matched controls with clinically health oral mucosa. Anamnestic charts designed for this study included information about symptoms (measured by Visual Analogue Scale), previous knowledge or fear about presence of GT and received therapy for this condition. Detection of Candida spp. from tongue was done using sterile cotton swab and Sabouraud dextrose agar. Results: At the time of this study, 18 (52.94%) of subjects with GT were aware of having this condition and even 12 of them (66.7%) used topical antifungal drugs prescribed by physician. Also, 66.7% of them experienced fear about the presence of GT at some moment. Detection of Candida spp. was similarly distributed in both groups (22.2% in group A and 17.6% in group B). Conclusions: Geographic tongue has frequently been treated by topical antifungal drugs. In this study, GT was not associated with presence of Candida spp.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Dental Medicine",
title = "Geographic tongue: Does Candida play a role in its pathogenesis",
volume = "23",
number = "3",
pages = "152-156",
doi = "10.2478/bjdm-2019-0027"
}

Matić-Petrović, S., Đorđević, M., Radunović, M., Živanović, T., Pavlica, D.,& Pucar, A.. (2019). Geographic tongue: Does Candida play a role in its pathogenesis. in Balkan Journal of Dental Medicine
Udruženje stomatologa Balkana., 23(3), 152-156.
https://doi.org/10.2478/bjdm-2019-0027

Matić-Petrović S, Đorđević M, Radunović M, Živanović T, Pavlica D, Pucar A. Geographic tongue: Does Candida play a role in its pathogenesis. in Balkan Journal of Dental Medicine. 2019;23(3):152-156.
doi:10.2478/bjdm-2019-0027 .

Matić-Petrović, Sanja, Đorđević, Mihajlo, Radunović, Milena, Živanović, Tanja, Pavlica, Dušan, Pucar, Ana, "Geographic tongue: Does Candida play a role in its pathogenesis" in Balkan Journal of Dental Medicine, 23, no. 3 (2019):152-156,
https://doi.org/10.2478/bjdm-2019-0027 . .

TY  - JOUR
AU  - Toljić, Boško
AU  - Trbovich, Alexander M.
AU  - Matić-Petrović, Sanja
AU  - Kannosh, Ibrahim
AU  - Dragović, Gordana
AU  - Jevtović, Đorđe
AU  - de Luka, Silvio R.
AU  - Ristic-Đurović, Jasna L.
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2269
AB  - The importance of oral microflora composition in HIV-infected patients is well recognized. However, no studies so far have dealt with age-related changes in periodontal pathogens occurrence in HIV+ individuals. The aim of the present study was to assess and compare temporal changes of bacteria frequency in younger ( lt = 35 years) and older (>= 50 years) HIV-infected and non-infected individuals. Bacterial DNA was isolated from buccal swabs of 30 younger and 30 older subjects in both HIV+ and HIV-groups. By means of PCR the following microorganisms were detected: Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Peptostreptococcus micros, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and Treponema denticola. Oral and periodontal examinations were performed in all subjects. The prevalence of microorganisms was significantly higher in HIV+ patients compared to controls, and their distribution showed a notable shift. The decreasing incidence in HIV-subjects was: Pi>Pm>Pg>Aa>Ec>Tf>Td whilst in HIV+ it was: Pi>Pm>Ec>Pg>Tf>Aa>Td. Oral manifestations of HIV infection were more frequent in older compared to younger patients. All measured values of clinical periodontal parameters were significantly higher in older compared to younger HIV+ patients. Ageing in HIV+ subjects is accompanied with a substantial increase and rearrangements of periodontal microflora, potentially aggravating oral and systemic health.
PB  - Luigi Ponzio e figlio Editori
T2  - New Microbiologica
T1  - Ageing with HIV - a periodontal perspective
VL  - 41
IS  - 1
SP  - 61
EP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_smile_2269
ER  - 

@article{
author = "Toljić, Boško and Trbovich, Alexander M. and Matić-Petrović, Sanja and Kannosh, Ibrahim and Dragović, Gordana and Jevtović, Đorđe and de Luka, Silvio R. and Ristic-Đurović, Jasna L. and Milašin, Jelena",
year = "2018",
abstract = "The importance of oral microflora composition in HIV-infected patients is well recognized. However, no studies so far have dealt with age-related changes in periodontal pathogens occurrence in HIV+ individuals. The aim of the present study was to assess and compare temporal changes of bacteria frequency in younger ( lt = 35 years) and older (>= 50 years) HIV-infected and non-infected individuals. Bacterial DNA was isolated from buccal swabs of 30 younger and 30 older subjects in both HIV+ and HIV-groups. By means of PCR the following microorganisms were detected: Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Peptostreptococcus micros, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and Treponema denticola. Oral and periodontal examinations were performed in all subjects. The prevalence of microorganisms was significantly higher in HIV+ patients compared to controls, and their distribution showed a notable shift. The decreasing incidence in HIV-subjects was: Pi>Pm>Pg>Aa>Ec>Tf>Td whilst in HIV+ it was: Pi>Pm>Ec>Pg>Tf>Aa>Td. Oral manifestations of HIV infection were more frequent in older compared to younger patients. All measured values of clinical periodontal parameters were significantly higher in older compared to younger HIV+ patients. Ageing in HIV+ subjects is accompanied with a substantial increase and rearrangements of periodontal microflora, potentially aggravating oral and systemic health.",
publisher = "Luigi Ponzio e figlio Editori",
journal = "New Microbiologica",
title = "Ageing with HIV - a periodontal perspective",
volume = "41",
number = "1",
pages = "61-66",
url = "https://hdl.handle.net/21.15107/rcub_smile_2269"
}

Toljić, B., Trbovich, A. M., Matić-Petrović, S., Kannosh, I., Dragović, G., Jevtović, Đ., de Luka, S. R., Ristic-Đurović, J. L.,& Milašin, J.. (2018). Ageing with HIV - a periodontal perspective. in New Microbiologica
Luigi Ponzio e figlio Editori., 41(1), 61-66.
https://hdl.handle.net/21.15107/rcub_smile_2269

Toljić B, Trbovich AM, Matić-Petrović S, Kannosh I, Dragović G, Jevtović Đ, de Luka SR, Ristic-Đurović JL, Milašin J. Ageing with HIV - a periodontal perspective. in New Microbiologica. 2018;41(1):61-66.
https://hdl.handle.net/21.15107/rcub_smile_2269 .

Toljić, Boško, Trbovich, Alexander M., Matić-Petrović, Sanja, Kannosh, Ibrahim, Dragović, Gordana, Jevtović, Đorđe, de Luka, Silvio R., Ristic-Đurović, Jasna L., Milašin, Jelena, "Ageing with HIV - a periodontal perspective" in New Microbiologica, 41, no. 1 (2018):61-66,
https://hdl.handle.net/21.15107/rcub_smile_2269 .

TY  - JOUR
AU  - Kannosh, Ibrahim
AU  - Staletović, Danijela
AU  - Toljić, Boško
AU  - Radunović, Milena
AU  - Pucar, Ana
AU  - Matić-Petrović, Sanja
AU  - Grubiša, Ivana
AU  - Lazarević, Miloš
AU  - Brkić, Zlata
AU  - Knežević-Vukcević, Jelena
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2276
AB  - Introduction: There is a known connection between periodontitis and atherosclerosis and the presence of periopathogens in blood vessels. However, changes of the oral microflora related to the aging process and its possible effects on atherosclerosis, have yet to be analyzed. The aim of this study was to assess temporal changes in the frequency of periodontal bacteria in the subgingival plaque and in atherosclerotic blood vessels of patients with atherosclerosis. Methodology: The study included 100 patients with atherosclerosis and periodontitis, divided into two groups, below and over 60 years of age. Clinical examinations were performedand subgingival plaque specimens were collected as well as biopsy specimens from the following arteries: coronary (34), carotid (29), abdominal (10), femoral (10), mammary (13) and iliac (4). Subgingival and artery specimens were subjected to PCR detection of 5 major periodontal pathogens: Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Aggregatibacter actinomycetemcomitans (Aa), Tannerella forsythensis (Tf) and Treponema denticola (Td). Results: Tf was the most and Td the least frequent bacteria in both age groups and in both types of samples. The frequencies of bacteria in subgingival versus atherosclerotic samples were: Tf (76%: 53%), Pi (71%: 31%), Pg (60%: 38%), Aa (39%: 14%) and Td (21%: 6%). Only Aa and Pi showed a significant difference of prevalence between younger and older patients. The most colonized artery was a. coronaria, followed by a. carotis, a. abdominalis, a. mammaria, and a. femoralis. Conclusions: Patient's age and the distance of a given blood vessel from the oral cavity influenced microbiological findings in the atherotic plaque.
PB  - J Infection Developing Countries, Tramaniglio
T2  - Journal of Infection in Developing Countries
T1  - The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis
VL  - 12
IS  - 12
SP  - 1088
EP  - 1095
DO  - 10.3855/jidc.10980
ER  - 

@article{
author = "Kannosh, Ibrahim and Staletović, Danijela and Toljić, Boško and Radunović, Milena and Pucar, Ana and Matić-Petrović, Sanja and Grubiša, Ivana and Lazarević, Miloš and Brkić, Zlata and Knežević-Vukcević, Jelena and Milašin, Jelena",
year = "2018",
abstract = "Introduction: There is a known connection between periodontitis and atherosclerosis and the presence of periopathogens in blood vessels. However, changes of the oral microflora related to the aging process and its possible effects on atherosclerosis, have yet to be analyzed. The aim of this study was to assess temporal changes in the frequency of periodontal bacteria in the subgingival plaque and in atherosclerotic blood vessels of patients with atherosclerosis. Methodology: The study included 100 patients with atherosclerosis and periodontitis, divided into two groups, below and over 60 years of age. Clinical examinations were performedand subgingival plaque specimens were collected as well as biopsy specimens from the following arteries: coronary (34), carotid (29), abdominal (10), femoral (10), mammary (13) and iliac (4). Subgingival and artery specimens were subjected to PCR detection of 5 major periodontal pathogens: Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Aggregatibacter actinomycetemcomitans (Aa), Tannerella forsythensis (Tf) and Treponema denticola (Td). Results: Tf was the most and Td the least frequent bacteria in both age groups and in both types of samples. The frequencies of bacteria in subgingival versus atherosclerotic samples were: Tf (76%: 53%), Pi (71%: 31%), Pg (60%: 38%), Aa (39%: 14%) and Td (21%: 6%). Only Aa and Pi showed a significant difference of prevalence between younger and older patients. The most colonized artery was a. coronaria, followed by a. carotis, a. abdominalis, a. mammaria, and a. femoralis. Conclusions: Patient's age and the distance of a given blood vessel from the oral cavity influenced microbiological findings in the atherotic plaque.",
publisher = "J Infection Developing Countries, Tramaniglio",
journal = "Journal of Infection in Developing Countries",
title = "The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis",
volume = "12",
number = "12",
pages = "1088-1095",
doi = "10.3855/jidc.10980"
}

Kannosh, I., Staletović, D., Toljić, B., Radunović, M., Pucar, A., Matić-Petrović, S., Grubiša, I., Lazarević, M., Brkić, Z., Knežević-Vukcević, J.,& Milašin, J.. (2018). The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis. in Journal of Infection in Developing Countries
J Infection Developing Countries, Tramaniglio., 12(12), 1088-1095.
https://doi.org/10.3855/jidc.10980

Kannosh I, Staletović D, Toljić B, Radunović M, Pucar A, Matić-Petrović S, Grubiša I, Lazarević M, Brkić Z, Knežević-Vukcević J, Milašin J. The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis. in Journal of Infection in Developing Countries. 2018;12(12):1088-1095.
doi:10.3855/jidc.10980 .

Kannosh, Ibrahim, Staletović, Danijela, Toljić, Boško, Radunović, Milena, Pucar, Ana, Matić-Petrović, Sanja, Grubiša, Ivana, Lazarević, Miloš, Brkić, Zlata, Knežević-Vukcević, Jelena, Milašin, Jelena, "The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis" in Journal of Infection in Developing Countries, 12, no. 12 (2018):1088-1095,
https://doi.org/10.3855/jidc.10980 . .

TY  - CONF
AU  - Radunović, Milena
AU  - Matić-Petrović, Sanja
AU  - Cimbaljević, Milena
AU  - Kuzmanović-Pfićer, Jovana
AU  - Pavlica, Dušan
AU  - Arsić-Arsenijević, Valentina
AU  - Pucar, Ana
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2295
PB  - Oxford Univ Press, Oxford
C3  - Medical Mycology
T1  - Subgingival sites and oral cavity as reservoirs for Candida spp. in diabetics and non-diabetics
VL  - 56
SP  - S108
EP  - S108
UR  - https://hdl.handle.net/21.15107/rcub_smile_2295
ER  - 

@conference{
author = "Radunović, Milena and Matić-Petrović, Sanja and Cimbaljević, Milena and Kuzmanović-Pfićer, Jovana and Pavlica, Dušan and Arsić-Arsenijević, Valentina and Pucar, Ana",
year = "2018",
publisher = "Oxford Univ Press, Oxford",
journal = "Medical Mycology",
title = "Subgingival sites and oral cavity as reservoirs for Candida spp. in diabetics and non-diabetics",
volume = "56",
pages = "S108-S108",
url = "https://hdl.handle.net/21.15107/rcub_smile_2295"
}

Radunović, M., Matić-Petrović, S., Cimbaljević, M., Kuzmanović-Pfićer, J., Pavlica, D., Arsić-Arsenijević, V.,& Pucar, A.. (2018). Subgingival sites and oral cavity as reservoirs for Candida spp. in diabetics and non-diabetics. in Medical Mycology
Oxford Univ Press, Oxford., 56, S108-S108.
https://hdl.handle.net/21.15107/rcub_smile_2295

Radunović M, Matić-Petrović S, Cimbaljević M, Kuzmanović-Pfićer J, Pavlica D, Arsić-Arsenijević V, Pucar A. Subgingival sites and oral cavity as reservoirs for Candida spp. in diabetics and non-diabetics. in Medical Mycology. 2018;56:S108-S108.
https://hdl.handle.net/21.15107/rcub_smile_2295 .

Radunović, Milena, Matić-Petrović, Sanja, Cimbaljević, Milena, Kuzmanović-Pfićer, Jovana, Pavlica, Dušan, Arsić-Arsenijević, Valentina, Pucar, Ana, "Subgingival sites and oral cavity as reservoirs for Candida spp. in diabetics and non-diabetics" in Medical Mycology, 56 (2018):S108-S108,
https://hdl.handle.net/21.15107/rcub_smile_2295 .

TY  - JOUR
AU  - Matić-Petrović, Sanja
AU  - Barać, Milena
AU  - Kuzmanović-Pfićer, Jovana
AU  - Radunović, Milena
AU  - Jotić, Aleksandra
AU  - Pucar, Ana
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2365
AB  - Background/Aim: The aim of this study was to examine prevalence of different Candida spp. at diabetics and nondiabetics wearing dentures without clinical signs of Denture Stomatitis (DS) and to study if some local and systematic factors are confounders for harboring Candida at these subjects. Material and Methods: Total of 60 subjects wearing partial or complete upper acrylic denture having at least half of palatal mucosa covered by denture were selected and stratified into three experimental groups: systematically health subjects; patients with diagnosed Type 2 Diabetes (T2D) and good glycoregulation; and T2D subjects with poorly regulated blood sugar level. Cotton swab samples were obtained from each patient from hard palate mucosa and denture surface. Swab cultures were made on Sabouraud dextrose agar and ChromAgar Media for distinciton of various Candida spp. Density growth was also measured. Results: Frequency of Candida spp. findings were similar between groups. At healthy subjects, only C.albicans was detected. At diabetics, C.albicans was the most common isolated species, followed by C.glabrata and C.tropicalis. Negative finding of yeasts on palatal mucosa, but positive on denture surface were detected at all groups, with the highest frequency (33.4%) at diabetics with poor glycoregulation. Denture surface was heavier colonized than hard palate mucosa. Duration of diabetes in years were only independent predictors for harboring Candida spp. at denture surface (Exp B=1.186, CI=1.047-1.344, p=0.007). Conclusions: Prosthesis of denture wearers without DS may serve as reservoir of Candida spp. Presence of more pathogenic and resistant non-albicans species are related to diabetics, even without clinical signs of DS.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Dental Medicine
T1  - Presence of different Candida species at denture wearers with type 2 diabetes and clinically healthy oral mucosa: Pilot study
VL  - 22
IS  - 1
SP  - 15
EP  - 21
DO  - 10.2478/bjdm-2018-0003
ER  - 

@article{
author = "Matić-Petrović, Sanja and Barać, Milena and Kuzmanović-Pfićer, Jovana and Radunović, Milena and Jotić, Aleksandra and Pucar, Ana",
year = "2018",
abstract = "Background/Aim: The aim of this study was to examine prevalence of different Candida spp. at diabetics and nondiabetics wearing dentures without clinical signs of Denture Stomatitis (DS) and to study if some local and systematic factors are confounders for harboring Candida at these subjects. Material and Methods: Total of 60 subjects wearing partial or complete upper acrylic denture having at least half of palatal mucosa covered by denture were selected and stratified into three experimental groups: systematically health subjects; patients with diagnosed Type 2 Diabetes (T2D) and good glycoregulation; and T2D subjects with poorly regulated blood sugar level. Cotton swab samples were obtained from each patient from hard palate mucosa and denture surface. Swab cultures were made on Sabouraud dextrose agar and ChromAgar Media for distinciton of various Candida spp. Density growth was also measured. Results: Frequency of Candida spp. findings were similar between groups. At healthy subjects, only C.albicans was detected. At diabetics, C.albicans was the most common isolated species, followed by C.glabrata and C.tropicalis. Negative finding of yeasts on palatal mucosa, but positive on denture surface were detected at all groups, with the highest frequency (33.4%) at diabetics with poor glycoregulation. Denture surface was heavier colonized than hard palate mucosa. Duration of diabetes in years were only independent predictors for harboring Candida spp. at denture surface (Exp B=1.186, CI=1.047-1.344, p=0.007). Conclusions: Prosthesis of denture wearers without DS may serve as reservoir of Candida spp. Presence of more pathogenic and resistant non-albicans species are related to diabetics, even without clinical signs of DS.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Dental Medicine",
title = "Presence of different Candida species at denture wearers with type 2 diabetes and clinically healthy oral mucosa: Pilot study",
volume = "22",
number = "1",
pages = "15-21",
doi = "10.2478/bjdm-2018-0003"
}

Matić-Petrović, S., Barać, M., Kuzmanović-Pfićer, J., Radunović, M., Jotić, A.,& Pucar, A.. (2018). Presence of different Candida species at denture wearers with type 2 diabetes and clinically healthy oral mucosa: Pilot study. in Balkan Journal of Dental Medicine
Udruženje stomatologa Balkana., 22(1), 15-21.
https://doi.org/10.2478/bjdm-2018-0003

Matić-Petrović S, Barać M, Kuzmanović-Pfićer J, Radunović M, Jotić A, Pucar A. Presence of different Candida species at denture wearers with type 2 diabetes and clinically healthy oral mucosa: Pilot study. in Balkan Journal of Dental Medicine. 2018;22(1):15-21.
doi:10.2478/bjdm-2018-0003 .

Matić-Petrović, Sanja, Barać, Milena, Kuzmanović-Pfićer, Jovana, Radunović, Milena, Jotić, Aleksandra, Pucar, Ana, "Presence of different Candida species at denture wearers with type 2 diabetes and clinically healthy oral mucosa: Pilot study" in Balkan Journal of Dental Medicine, 22, no. 1 (2018):15-21,
https://doi.org/10.2478/bjdm-2018-0003 . .

TY  - CONF
AU  - Matić-Petrović, Sanja
AU  - Jovelić, N.
AU  - Toljić, Boško
AU  - Đorđević, M.
AU  - Pucar, Ana
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2152
PB  - Wiley, Hoboken
C3  - Oral Diseases
T1  - TNFRs polymorphism and serum concentrations in periodontitis and diabetes
VL  - 22
SP  - 33
EP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_smile_2152
ER  - 

@conference{
author = "Matić-Petrović, Sanja and Jovelić, N. and Toljić, Boško and Đorđević, M. and Pucar, Ana",
year = "2016",
publisher = "Wiley, Hoboken",
journal = "Oral Diseases",
title = "TNFRs polymorphism and serum concentrations in periodontitis and diabetes",
volume = "22",
pages = "33-33",
url = "https://hdl.handle.net/21.15107/rcub_smile_2152"
}

Matić-Petrović, S., Jovelić, N., Toljić, B., Đorđević, M.,& Pucar, A.. (2016). TNFRs polymorphism and serum concentrations in periodontitis and diabetes. in Oral Diseases
Wiley, Hoboken., 22, 33-33.
https://hdl.handle.net/21.15107/rcub_smile_2152

Matić-Petrović S, Jovelić N, Toljić B, Đorđević M, Pucar A. TNFRs polymorphism and serum concentrations in periodontitis and diabetes. in Oral Diseases. 2016;22:33-33.
https://hdl.handle.net/21.15107/rcub_smile_2152 .

Matić-Petrović, Sanja, Jovelić, N., Toljić, Boško, Đorđević, M., Pucar, Ana, "TNFRs polymorphism and serum concentrations in periodontitis and diabetes" in Oral Diseases, 22 (2016):33-33,
https://hdl.handle.net/21.15107/rcub_smile_2152 .

TY  - JOUR
AU  - Matić-Petrović, Sanja
AU  - Pucar, Ana
AU  - Jotić, Aleksandra
AU  - Miličić, Biljana
AU  - Arambašić-Jovanović, Jelena
AU  - Vidaković, Melita
AU  - Leković, Vojislav
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2158
AB  - Introduction: The role of tumor necrosis factor-α (TNFα) is well documented in pathogenesis of chronic periodontitis (CP) and type 2 diabetes (T2D). Considering short half-life of TNFα, tumor necrosis factor receptor-2 (TNFR2) is used as prosperous surrogate marker of TNFα activity. Objective The aim was to detect TNFR2 serum concentration and correlate it with periodontal destruction in patients with diagnosed T2D and nondiabetics. Methods The study included 85 patients divided into three groups: T2D + CP (group T2D, n = 34); nondiabetics + CP (Group PD, n = 27); and healthy controls (group HC, n = 24). T2D was diagnosed according to WHO criteria (2013) and periodontitis was diagnosed using International Workshop for a Classification of Periodontal Diseases and Conditions criteria (1999). TNFR2 level was measured by enzyme-linked immunosorbent assay (ELISA). Results There was no difference in TNFR2 level among the groups (Kruskal-Wallis, p = 0.482). Significant correlation (Pearson's correlation coefficient) was observed between clinical attachment loss (CAL) and TNFR2 concentration in PD group (rp = -0.460, p = 0.016). In T2D group, correlations were observed between TNFR2 concentration and CaL (rp = 0.363, p = 0.005) and periodontal inflamed surface area (PISA) (rp = 0.345, p = 0.046) and periodontalepithelial surface area (PESA) (rp = 0.578, p = 0.000). Conclusion Higher concentration of TNFR2 was associated with higher CAL, PESA, and PISA scores in T2D group. Contrary to that, nondiabetics with higher values of CAL exhibited lower concentration of TNFR2, presenting potential protective effect on periodontal destruction. These results imply that diabetes may alter TNFR2 secretion originated from periodontium.
AB  - Uvod: Uloga faktora nekroze tumora-alfa (TNFα) dokazana je u patogenezi hronične parodontopatije (HP) i dijabetesa melitusa tipa 2 (DM tip 2). S obzirom na to da je poluživot TNFα veoma kratak, receptor 2 faktora nekroze tumora (TNFR2) koristi se kao marker aktivnosti TNFα. Cilj rada Cilj ovog rada je određivanje koncentracije TNFR2 u serumu i koreliranje sa parametrima destrukcije parodoncijuma kod zdravih i ispitanika sa dijagnostikovanim DM tip 2. Metode rada U studiju je uključeno 85 pacijenata podeljenih u tri grupe: DM tip 2 + HP (DM grupa, n = 34), zdravi ispitanici + HP (PD grupa, n = 27) i zdrave kontrole (ZK grupa, n = 24). Dijagnoza DM tip 2 postavljena je na osnovu kriterijuma SZO (2013), dok je dijagnoza HP postavljena na osnovu kriterijuma Internacionalne radionice za klasifikaciju stanja i oboljenja parodoncijuma (1999). Koncentracija TNFR2 merena je ELISA metodom. Rezultati Koncentracija serumskog TNFR2 nije se razlikovala među grupama (Kraskal-Volis, p = 0,482). Postoji značajna korelacija (Pirson) između nivoa pripojnog epitela (NPE) i koncentracije TNFR2 u PD grupi (rp = -0,460, p = 0,016). U DM tip 2 grupi, statistički značajna korelacija uočena je između koncentracije TNFR2 i NPE (rp = 0,363, p = 0,005), kao i parametara uticaja inflamacije iz parodoncijuma na sistemsko zdravlje - PISA (rp = 0,345, p = 0,046) i PESA (rp = 0,578, p = 0,000). Zaključak Kod pacijenata sa dijabetesom veće koncentracije TNFR2 odgovaraju većim vrednostima NPE, PESA i PISA. Nasuprot tome, kod sistemski zdravih ispitanika sa HP veće vrednosti NPE su povezane sa manjim koncentracijama TNFR2, što bi moglo govoriti o potencijalnoj zaštitnoj ulozi ovog citokina na destrukciju parodoncijuma. Rezultati govore da dijabetes može uticati na sekreciju TNFR2 iz parodoncijuma.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study
T1  - Korelacija između koncentracije receptora 2 faktora nekroze tumora u serumu i destrukcije parodoncijuma kod bolesnika sa dijabetes melitusom tip 2 - studija preseka
VL  - 144
IS  - 5-6
SP  - 266
EP  - 272
DO  - 10.2298/SARH1606266M
ER  - 

@article{
author = "Matić-Petrović, Sanja and Pucar, Ana and Jotić, Aleksandra and Miličić, Biljana and Arambašić-Jovanović, Jelena and Vidaković, Melita and Leković, Vojislav",
year = "2016",
abstract = "Introduction: The role of tumor necrosis factor-α (TNFα) is well documented in pathogenesis of chronic periodontitis (CP) and type 2 diabetes (T2D). Considering short half-life of TNFα, tumor necrosis factor receptor-2 (TNFR2) is used as prosperous surrogate marker of TNFα activity. Objective The aim was to detect TNFR2 serum concentration and correlate it with periodontal destruction in patients with diagnosed T2D and nondiabetics. Methods The study included 85 patients divided into three groups: T2D + CP (group T2D, n = 34); nondiabetics + CP (Group PD, n = 27); and healthy controls (group HC, n = 24). T2D was diagnosed according to WHO criteria (2013) and periodontitis was diagnosed using International Workshop for a Classification of Periodontal Diseases and Conditions criteria (1999). TNFR2 level was measured by enzyme-linked immunosorbent assay (ELISA). Results There was no difference in TNFR2 level among the groups (Kruskal-Wallis, p = 0.482). Significant correlation (Pearson's correlation coefficient) was observed between clinical attachment loss (CAL) and TNFR2 concentration in PD group (rp = -0.460, p = 0.016). In T2D group, correlations were observed between TNFR2 concentration and CaL (rp = 0.363, p = 0.005) and periodontal inflamed surface area (PISA) (rp = 0.345, p = 0.046) and periodontalepithelial surface area (PESA) (rp = 0.578, p = 0.000). Conclusion Higher concentration of TNFR2 was associated with higher CAL, PESA, and PISA scores in T2D group. Contrary to that, nondiabetics with higher values of CAL exhibited lower concentration of TNFR2, presenting potential protective effect on periodontal destruction. These results imply that diabetes may alter TNFR2 secretion originated from periodontium., Uvod: Uloga faktora nekroze tumora-alfa (TNFα) dokazana je u patogenezi hronične parodontopatije (HP) i dijabetesa melitusa tipa 2 (DM tip 2). S obzirom na to da je poluživot TNFα veoma kratak, receptor 2 faktora nekroze tumora (TNFR2) koristi se kao marker aktivnosti TNFα. Cilj rada Cilj ovog rada je određivanje koncentracije TNFR2 u serumu i koreliranje sa parametrima destrukcije parodoncijuma kod zdravih i ispitanika sa dijagnostikovanim DM tip 2. Metode rada U studiju je uključeno 85 pacijenata podeljenih u tri grupe: DM tip 2 + HP (DM grupa, n = 34), zdravi ispitanici + HP (PD grupa, n = 27) i zdrave kontrole (ZK grupa, n = 24). Dijagnoza DM tip 2 postavljena je na osnovu kriterijuma SZO (2013), dok je dijagnoza HP postavljena na osnovu kriterijuma Internacionalne radionice za klasifikaciju stanja i oboljenja parodoncijuma (1999). Koncentracija TNFR2 merena je ELISA metodom. Rezultati Koncentracija serumskog TNFR2 nije se razlikovala među grupama (Kraskal-Volis, p = 0,482). Postoji značajna korelacija (Pirson) između nivoa pripojnog epitela (NPE) i koncentracije TNFR2 u PD grupi (rp = -0,460, p = 0,016). U DM tip 2 grupi, statistički značajna korelacija uočena je između koncentracije TNFR2 i NPE (rp = 0,363, p = 0,005), kao i parametara uticaja inflamacije iz parodoncijuma na sistemsko zdravlje - PISA (rp = 0,345, p = 0,046) i PESA (rp = 0,578, p = 0,000). Zaključak Kod pacijenata sa dijabetesom veće koncentracije TNFR2 odgovaraju većim vrednostima NPE, PESA i PISA. Nasuprot tome, kod sistemski zdravih ispitanika sa HP veće vrednosti NPE su povezane sa manjim koncentracijama TNFR2, što bi moglo govoriti o potencijalnoj zaštitnoj ulozi ovog citokina na destrukciju parodoncijuma. Rezultati govore da dijabetes može uticati na sekreciju TNFR2 iz parodoncijuma.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study, Korelacija između koncentracije receptora 2 faktora nekroze tumora u serumu i destrukcije parodoncijuma kod bolesnika sa dijabetes melitusom tip 2 - studija preseka",
volume = "144",
number = "5-6",
pages = "266-272",
doi = "10.2298/SARH1606266M"
}

Matić-Petrović, S., Pucar, A., Jotić, A., Miličić, B., Arambašić-Jovanović, J., Vidaković, M.,& Leković, V.. (2016). Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 144(5-6), 266-272.
https://doi.org/10.2298/SARH1606266M

Matić-Petrović S, Pucar A, Jotić A, Miličić B, Arambašić-Jovanović J, Vidaković M, Leković V. Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study. in Srpski arhiv za celokupno lekarstvo. 2016;144(5-6):266-272.
doi:10.2298/SARH1606266M .

Matić-Petrović, Sanja, Pucar, Ana, Jotić, Aleksandra, Miličić, Biljana, Arambašić-Jovanović, Jelena, Vidaković, Melita, Leković, Vojislav, "Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study" in Srpski arhiv za celokupno lekarstvo, 144, no. 5-6 (2016):266-272,
https://doi.org/10.2298/SARH1606266M . .

TY  - JOUR
AU  - Grdović, Nevena
AU  - Rajić, Jovana
AU  - Matić-Petrović, Sanja
AU  - Dinić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Mihailović, Mirjana
AU  - Arambašić-Jovanović, Jelena
AU  - Tolić, Anja
AU  - Pucar, Ana
AU  - Milašin, Jelena
AU  - Vidaković, Melita
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2122
AB  - Objectives: CXCL12 is widely expressed, constitutive chemokine involved in tissue repair and regeneration, while the extent of its expression is important in various chronic inflammatory conditions. Involvement of DNA methylation in CXCL12 gene suppression (CXCL12) has been shown in malignancy and some autoimmune diseases. The aim of this study was to investigate whether the alterations in DNA methylation of CXCL12 are also involved in progression of periodontitis in combination with diabetes, as these chronic inflammatory conditions are strongly interrelated. Design: Study included 72 subjects divided in three groups: healthy control (C, n=21), periodontitis (P, n=29) and diabetes/periodontitis group (D/P, n=22). DNA extracted from epithelial cells obtained by sterile cotton swabs from buccal mucosa was subjected to methylation specific polymerase chain reaction (MSP) to obtain DNA methylation pattern of CXCL12 promoter. Results: CXCL12 promoter was predominantly unmethylated in all groups. However, increase in the frequency of the methylated form and increase in percent of methylation of CXCL12 promoter in periodontitis and diabetes/periodontitis group compared to control group were found, although without statistical significance. However, statistically significant increase in Tm of MSP products in diabetes/periodontitis group was observed. Correlation analysis revealed statistically significant relationship between the extent of DNA methylation of the CXCL12 promoter and periodontal parameters, as well as between DNA methylation of CXCL12 and glycosylated hemoglobin. Conclusion: Presented results suggest that chronic inflammation contributes to the change of CXCL12 DNA methylation in buccal cells and that DNA methylation profile of CXCL12 promoter plays important role in development and progression of periodontal disease.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Archives of Oral Biology
T1  - Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2
VL  - 72
SP  - 124
EP  - 133
DO  - 10.1016/j.archoralbio.2016.08.025
ER  - 

@article{
author = "Grdović, Nevena and Rajić, Jovana and Matić-Petrović, Sanja and Dinić, Svetlana and Uskoković, Aleksandra and Mihailović, Mirjana and Arambašić-Jovanović, Jelena and Tolić, Anja and Pucar, Ana and Milašin, Jelena and Vidaković, Melita",
year = "2016",
abstract = "Objectives: CXCL12 is widely expressed, constitutive chemokine involved in tissue repair and regeneration, while the extent of its expression is important in various chronic inflammatory conditions. Involvement of DNA methylation in CXCL12 gene suppression (CXCL12) has been shown in malignancy and some autoimmune diseases. The aim of this study was to investigate whether the alterations in DNA methylation of CXCL12 are also involved in progression of periodontitis in combination with diabetes, as these chronic inflammatory conditions are strongly interrelated. Design: Study included 72 subjects divided in three groups: healthy control (C, n=21), periodontitis (P, n=29) and diabetes/periodontitis group (D/P, n=22). DNA extracted from epithelial cells obtained by sterile cotton swabs from buccal mucosa was subjected to methylation specific polymerase chain reaction (MSP) to obtain DNA methylation pattern of CXCL12 promoter. Results: CXCL12 promoter was predominantly unmethylated in all groups. However, increase in the frequency of the methylated form and increase in percent of methylation of CXCL12 promoter in periodontitis and diabetes/periodontitis group compared to control group were found, although without statistical significance. However, statistically significant increase in Tm of MSP products in diabetes/periodontitis group was observed. Correlation analysis revealed statistically significant relationship between the extent of DNA methylation of the CXCL12 promoter and periodontal parameters, as well as between DNA methylation of CXCL12 and glycosylated hemoglobin. Conclusion: Presented results suggest that chronic inflammation contributes to the change of CXCL12 DNA methylation in buccal cells and that DNA methylation profile of CXCL12 promoter plays important role in development and progression of periodontal disease.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Archives of Oral Biology",
title = "Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2",
volume = "72",
pages = "124-133",
doi = "10.1016/j.archoralbio.2016.08.025"
}

Grdović, N., Rajić, J., Matić-Petrović, S., Dinić, S., Uskoković, A., Mihailović, M., Arambašić-Jovanović, J., Tolić, A., Pucar, A., Milašin, J.,& Vidaković, M.. (2016). Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2. in Archives of Oral Biology
Pergamon-Elsevier Science Ltd, Oxford., 72, 124-133.
https://doi.org/10.1016/j.archoralbio.2016.08.025

Grdović N, Rajić J, Matić-Petrović S, Dinić S, Uskoković A, Mihailović M, Arambašić-Jovanović J, Tolić A, Pucar A, Milašin J, Vidaković M. Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2. in Archives of Oral Biology. 2016;72:124-133.
doi:10.1016/j.archoralbio.2016.08.025 .

Grdović, Nevena, Rajić, Jovana, Matić-Petrović, Sanja, Dinić, Svetlana, Uskoković, Aleksandra, Mihailović, Mirjana, Arambašić-Jovanović, Jelena, Tolić, Anja, Pucar, Ana, Milašin, Jelena, Vidaković, Melita, "Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2" in Archives of Oral Biology, 72 (2016):124-133,
https://doi.org/10.1016/j.archoralbio.2016.08.025 . .

TY  - JOUR
AU  - Matić-Petrović, Sanja
AU  - Cimbaljević, Milena
AU  - Radunović, Milena
AU  - Kuzmanović-Pfićer, Jovana
AU  - Jotić, Aleksandra
AU  - Pucar, Ana
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2052
AB  - The purpose of this study was to detect Candida spp. on the tongue and in the subgingival sites in healthy and type 2 diabetes (T2D) patients with chronic periodontitis (CP), and to compare the accuracy of sampling methods. This study included 131 patients divided into four groups: healthy control (group A), nondiabetics + CP (Group B), diabetics with good metabolic control + CP (group C) and diabetics with poor glycoregulation + CP (Group D). Cotton swab samples from tongue and subgingival samples were obtained from each patient with help of sterile paper points and a sterile curette. Swab cultures were made on Sabouraud dextrose agar. The number of CFUs was counted. The sampling methods for subgingival plaque were compared by Receiving Operator Curve (ROC). The presence of Candida spp. on the tongue was statistically significant among groups (group D vs. others three groups:.2: p  lt  0.005 for each group). Positive findings of subgingival Candida spp. did not differ among the groups. There were no significant differences in the quantification of Candida spp., neither on the tongue, nor in the subgingival samples. 17.2% of diabetic patients revealed the presence of Candida spp. in the subgingival samples, with negative finding on tongue. There was a significant difference in the sampling methods for subgingival plaque (p = 0.000). Candida spp. is more prevalent on the tongue of diabetics. The sampling of subgingival plaque by a sterile curette is more accurate than with paper points. Subgingival plaque may represent a reservoir of commensals. It is necessary to standardize the sampling of subgingival plaque.
PB  - Sociedade Brasileira De Pesquisa Odontologica, Sao Paulo
T2  - Brazilian Oral Research
T1  - Detection and sampling methods for isolation of Candida spp. from oral cavities in diabetics and non-diabetics
VL  - 29
IS  - 1
DO  - 10.1590/1807-3107BOR-2015.vol29.0077
ER  - 

@article{
author = "Matić-Petrović, Sanja and Cimbaljević, Milena and Radunović, Milena and Kuzmanović-Pfićer, Jovana and Jotić, Aleksandra and Pucar, Ana",
year = "2015",
abstract = "The purpose of this study was to detect Candida spp. on the tongue and in the subgingival sites in healthy and type 2 diabetes (T2D) patients with chronic periodontitis (CP), and to compare the accuracy of sampling methods. This study included 131 patients divided into four groups: healthy control (group A), nondiabetics + CP (Group B), diabetics with good metabolic control + CP (group C) and diabetics with poor glycoregulation + CP (Group D). Cotton swab samples from tongue and subgingival samples were obtained from each patient with help of sterile paper points and a sterile curette. Swab cultures were made on Sabouraud dextrose agar. The number of CFUs was counted. The sampling methods for subgingival plaque were compared by Receiving Operator Curve (ROC). The presence of Candida spp. on the tongue was statistically significant among groups (group D vs. others three groups:.2: p  lt  0.005 for each group). Positive findings of subgingival Candida spp. did not differ among the groups. There were no significant differences in the quantification of Candida spp., neither on the tongue, nor in the subgingival samples. 17.2% of diabetic patients revealed the presence of Candida spp. in the subgingival samples, with negative finding on tongue. There was a significant difference in the sampling methods for subgingival plaque (p = 0.000). Candida spp. is more prevalent on the tongue of diabetics. The sampling of subgingival plaque by a sterile curette is more accurate than with paper points. Subgingival plaque may represent a reservoir of commensals. It is necessary to standardize the sampling of subgingival plaque.",
publisher = "Sociedade Brasileira De Pesquisa Odontologica, Sao Paulo",
journal = "Brazilian Oral Research",
title = "Detection and sampling methods for isolation of Candida spp. from oral cavities in diabetics and non-diabetics",
volume = "29",
number = "1",
doi = "10.1590/1807-3107BOR-2015.vol29.0077"
}

Matić-Petrović, S., Cimbaljević, M., Radunović, M., Kuzmanović-Pfićer, J., Jotić, A.,& Pucar, A.. (2015). Detection and sampling methods for isolation of Candida spp. from oral cavities in diabetics and non-diabetics. in Brazilian Oral Research
Sociedade Brasileira De Pesquisa Odontologica, Sao Paulo., 29(1).
https://doi.org/10.1590/1807-3107BOR-2015.vol29.0077

Matić-Petrović S, Cimbaljević M, Radunović M, Kuzmanović-Pfićer J, Jotić A, Pucar A. Detection and sampling methods for isolation of Candida spp. from oral cavities in diabetics and non-diabetics. in Brazilian Oral Research. 2015;29(1).
doi:10.1590/1807-3107BOR-2015.vol29.0077 .

Matić-Petrović, Sanja, Cimbaljević, Milena, Radunović, Milena, Kuzmanović-Pfićer, Jovana, Jotić, Aleksandra, Pucar, Ana, "Detection and sampling methods for isolation of Candida spp. from oral cavities in diabetics and non-diabetics" in Brazilian Oral Research, 29, no. 1 (2015),
https://doi.org/10.1590/1807-3107BOR-2015.vol29.0077 . .

TY  - CONF
AU  - Matić-Petrović, Sanja
AU  - Milašin, Jelena
AU  - Vidaković, M.
AU  - Jotić, Aleksandra
AU  - Toljić, Boško
AU  - Miličić, Biljana
AU  - Pucar, Ana
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1933
PB  - Wiley-Blackwell, Hoboken
C3  - Oral Diseases
T1  - TNFR2 polymorphism and serum concentrations in periodontitis and diabetes
VL  - 20
SP  - 19
EP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_smile_1933
ER  - 

@conference{
author = "Matić-Petrović, Sanja and Milašin, Jelena and Vidaković, M. and Jotić, Aleksandra and Toljić, Boško and Miličić, Biljana and Pucar, Ana",
year = "2014",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Oral Diseases",
title = "TNFR2 polymorphism and serum concentrations in periodontitis and diabetes",
volume = "20",
pages = "19-19",
url = "https://hdl.handle.net/21.15107/rcub_smile_1933"
}

Matić-Petrović, S., Milašin, J., Vidaković, M., Jotić, A., Toljić, B., Miličić, B.,& Pucar, A.. (2014). TNFR2 polymorphism and serum concentrations in periodontitis and diabetes. in Oral Diseases
Wiley-Blackwell, Hoboken., 20, 19-19.
https://hdl.handle.net/21.15107/rcub_smile_1933

Matić-Petrović S, Milašin J, Vidaković M, Jotić A, Toljić B, Miličić B, Pucar A. TNFR2 polymorphism and serum concentrations in periodontitis and diabetes. in Oral Diseases. 2014;20:19-19.
https://hdl.handle.net/21.15107/rcub_smile_1933 .

Matić-Petrović, Sanja, Milašin, Jelena, Vidaković, M., Jotić, Aleksandra, Toljić, Boško, Miličić, Biljana, Pucar, Ana, "TNFR2 polymorphism and serum concentrations in periodontitis and diabetes" in Oral Diseases, 20 (2014):19-19,
https://hdl.handle.net/21.15107/rcub_smile_1933 .

TY  - CONF
AU  - Pucar, Ana
AU  - Matić-Petrović, Sanja
AU  - Radunović, Milena
AU  - Cimbaljević-Barac, M.
AU  - Kuzmanović-Pfićer, Jovana
AU  - Jotić, Aleksandra
AU  - Lalić, N.
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1928
PB  - Wiley-Blackwell, Hoboken
C3  - Oral Diseases
T1  - Presence and quantification of Candida spp in diabetics and nondiabetics
VL  - 20
SP  - 19
EP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_smile_1928
ER  - 

@conference{
author = "Pucar, Ana and Matić-Petrović, Sanja and Radunović, Milena and Cimbaljević-Barac, M. and Kuzmanović-Pfićer, Jovana and Jotić, Aleksandra and Lalić, N.",
year = "2014",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Oral Diseases",
title = "Presence and quantification of Candida spp in diabetics and nondiabetics",
volume = "20",
pages = "19-19",
url = "https://hdl.handle.net/21.15107/rcub_smile_1928"
}

Pucar, A., Matić-Petrović, S., Radunović, M., Cimbaljević-Barac, M., Kuzmanović-Pfićer, J., Jotić, A.,& Lalić, N.. (2014). Presence and quantification of Candida spp in diabetics and nondiabetics. in Oral Diseases
Wiley-Blackwell, Hoboken., 20, 19-19.
https://hdl.handle.net/21.15107/rcub_smile_1928

Pucar A, Matić-Petrović S, Radunović M, Cimbaljević-Barac M, Kuzmanović-Pfićer J, Jotić A, Lalić N. Presence and quantification of Candida spp in diabetics and nondiabetics. in Oral Diseases. 2014;20:19-19.
https://hdl.handle.net/21.15107/rcub_smile_1928 .

Pucar, Ana, Matić-Petrović, Sanja, Radunović, Milena, Cimbaljević-Barac, M., Kuzmanović-Pfićer, Jovana, Jotić, Aleksandra, Lalić, N., "Presence and quantification of Candida spp in diabetics and nondiabetics" in Oral Diseases, 20 (2014):19-19,
https://hdl.handle.net/21.15107/rcub_smile_1928 .