TY  - JOUR
AU  - Toljić, Boško
AU  - Milašin, Jelena
AU  - de Luka, Silvio R.
AU  - Dragović, Gordana
AU  - Jevtović, Đorđe
AU  - Maslać, Aleksandar
AU  - Ristić-Đurović, Jasna
AU  - Trbovich, Alexander M.
PY  - 2023
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/3281
AB  - We appraised the relationship between the biological and the chrono-
logical age and estimated the rate of biological aging in HIV-infected patients. Two
independent biomarkers, the relative telomere length and iron metabolism parame-
ters, were analyzed in younger (,35) and older (.50) HIV-infected and uninfected
patients (control group). In our control group, telomeres of younger patients were
significantly longer than telomeres of older ones. However, in HIV-infected partici-
pants, the difference in the length of telomeres was lost. By combining the length of
telomeres with serum iron, ferritin, and transferrin iron-binding capacity, a new for-
mula for determination of the aging process was developed. The life expectancy of
the healthy population was related to their biological age, and HIV-infected patients
were biologically older. The effect of antiretroviral HIV drug therapies varied with
respect to the biological aging process.
IMPORTANCE This article is focused on the dynamics of human aging. Moreover, its
interdisciplinary approach is applicable to various systems that are aging.
PB  - American Society for Microbiology
T2  - Microbiology Spectrum
T1  - HIV-Infected Patients as a Model of Aging
VL  - 11
IS  - 3
SP  - e00532-23
DO  - 10.1128/spectrum.00532-23
ER  - 

@article{
author = "Toljić, Boško and Milašin, Jelena and de Luka, Silvio R. and Dragović, Gordana and Jevtović, Đorđe and Maslać, Aleksandar and Ristić-Đurović, Jasna and Trbovich, Alexander M.",
year = "2023",
abstract = "We appraised the relationship between the biological and the chrono-
logical age and estimated the rate of biological aging in HIV-infected patients. Two
independent biomarkers, the relative telomere length and iron metabolism parame-
ters, were analyzed in younger (,35) and older (.50) HIV-infected and uninfected
patients (control group). In our control group, telomeres of younger patients were
significantly longer than telomeres of older ones. However, in HIV-infected partici-
pants, the difference in the length of telomeres was lost. By combining the length of
telomeres with serum iron, ferritin, and transferrin iron-binding capacity, a new for-
mula for determination of the aging process was developed. The life expectancy of
the healthy population was related to their biological age, and HIV-infected patients
were biologically older. The effect of antiretroviral HIV drug therapies varied with
respect to the biological aging process.
IMPORTANCE This article is focused on the dynamics of human aging. Moreover, its
interdisciplinary approach is applicable to various systems that are aging.",
publisher = "American Society for Microbiology",
journal = "Microbiology Spectrum",
title = "HIV-Infected Patients as a Model of Aging",
volume = "11",
number = "3",
pages = "e00532-23",
doi = "10.1128/spectrum.00532-23"
}

Toljić, B., Milašin, J., de Luka, S. R., Dragović, G., Jevtović, Đ., Maslać, A., Ristić-Đurović, J.,& Trbovich, A. M.. (2023). HIV-Infected Patients as a Model of Aging. in Microbiology Spectrum
American Society for Microbiology., 11(3), e00532-23.
https://doi.org/10.1128/spectrum.00532-23

Toljić B, Milašin J, de Luka SR, Dragović G, Jevtović Đ, Maslać A, Ristić-Đurović J, Trbovich AM. HIV-Infected Patients as a Model of Aging. in Microbiology Spectrum. 2023;11(3):e00532-23.
doi:10.1128/spectrum.00532-23 .

Toljić, Boško, Milašin, Jelena, de Luka, Silvio R., Dragović, Gordana, Jevtović, Đorđe, Maslać, Aleksandar, Ristić-Đurović, Jasna, Trbovich, Alexander M., "HIV-Infected Patients as a Model of Aging" in Microbiology Spectrum, 11, no. 3 (2023):e00532-23,
https://doi.org/10.1128/spectrum.00532-23 . .

TY  - JOUR
AU  - Simonović, Jelena
AU  - Toljić, Boško
AU  - Lazarević, Miloš
AU  - Milošević-Marković, Maja
AU  - Perić, Mina
AU  - Vujin, Jasna
AU  - Panajotović, Radmila
AU  - Milašin, Jelena
PY  - 2022
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/3282
AB  - Background: Dental stem cells, which originate from the neural crest, due to their easy
accessibility might be good candidates in neuro-regenerative procedures, along with graphene-based
nanomaterials shown to promote neurogenesis in vitro. We aimed to explore the potential of liquid-
phase exfoliated graphene (LPEG) film to stimulate the neuro-differentiation of stem cells from apical
papilla (SCAP). Methods: The experimental procedure was structured as follows: (1) fabrication
of graphene film; (2) isolation, cultivation and SCAP stemness characterization by flowcytometry,
multilineage differentiation (osteo, chondro and adipo) and quantitative PCR (qPCR); (3) SCAP
neuro-induction by cultivation on polyethylene terephthalate (PET) coated with graphene film;
(4) evaluation of neural differentiation by means of several microscopy techniques (light, confocal,
atomic force and scanning electron microscopy), followed by neural marker gene expression analysis
using qPCR. Results: SCAP demonstrated exceptional stemness, as judged by mesenchymal markers’
expression (CD73, CD90 and CD105), and by multilineage differentiation capacity (osteo, chondro and
adipo-differentiation). Neuro-induction of SCAP grown on PET coated with graphene film resulted
in neuron-like cellular phenotype observed under different microscopes. This was corroborated
by the high gene expression of all examined key neuronal markers (Ngn2, NF-M, Nestin, MAP2,
MASH1). Conclusions: The ability of SCAPs to differentiate toward neural lineages was markedly
enhanced by graphene film.
PB  - MDPI
T2  - Nanomaterials
T1  - The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla
VL  - 12
IS  - 18
SP  - 3116
DO  - 10.3390/nano12183116
ER  - 

@article{
author = "Simonović, Jelena and Toljić, Boško and Lazarević, Miloš and Milošević-Marković, Maja and Perić, Mina and Vujin, Jasna and Panajotović, Radmila and Milašin, Jelena",
year = "2022",
abstract = "Background: Dental stem cells, which originate from the neural crest, due to their easy
accessibility might be good candidates in neuro-regenerative procedures, along with graphene-based
nanomaterials shown to promote neurogenesis in vitro. We aimed to explore the potential of liquid-
phase exfoliated graphene (LPEG) film to stimulate the neuro-differentiation of stem cells from apical
papilla (SCAP). Methods: The experimental procedure was structured as follows: (1) fabrication
of graphene film; (2) isolation, cultivation and SCAP stemness characterization by flowcytometry,
multilineage differentiation (osteo, chondro and adipo) and quantitative PCR (qPCR); (3) SCAP
neuro-induction by cultivation on polyethylene terephthalate (PET) coated with graphene film;
(4) evaluation of neural differentiation by means of several microscopy techniques (light, confocal,
atomic force and scanning electron microscopy), followed by neural marker gene expression analysis
using qPCR. Results: SCAP demonstrated exceptional stemness, as judged by mesenchymal markers’
expression (CD73, CD90 and CD105), and by multilineage differentiation capacity (osteo, chondro and
adipo-differentiation). Neuro-induction of SCAP grown on PET coated with graphene film resulted
in neuron-like cellular phenotype observed under different microscopes. This was corroborated
by the high gene expression of all examined key neuronal markers (Ngn2, NF-M, Nestin, MAP2,
MASH1). Conclusions: The ability of SCAPs to differentiate toward neural lineages was markedly
enhanced by graphene film.",
publisher = "MDPI",
journal = "Nanomaterials",
title = "The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla",
volume = "12",
number = "18",
pages = "3116",
doi = "10.3390/nano12183116"
}

Simonović, J., Toljić, B., Lazarević, M., Milošević-Marković, M., Perić, M., Vujin, J., Panajotović, R.,& Milašin, J.. (2022). The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla. in Nanomaterials
MDPI., 12(18), 3116.
https://doi.org/10.3390/nano12183116

Simonović J, Toljić B, Lazarević M, Milošević-Marković M, Perić M, Vujin J, Panajotović R, Milašin J. The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla. in Nanomaterials. 2022;12(18):3116.
doi:10.3390/nano12183116 .

Simonović, Jelena, Toljić, Boško, Lazarević, Miloš, Milošević-Marković, Maja, Perić, Mina, Vujin, Jasna, Panajotović, Radmila, Milašin, Jelena, "The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla" in Nanomaterials, 12, no. 18 (2022):3116,
https://doi.org/10.3390/nano12183116 . .

TY  - JOUR
AU  - Milošević-Marković, Maja
AU  - Lazarević, Miloš
AU  - Toljić, Boško
AU  - Petrović, Milan
AU  - Vukadinović, Miroslav
AU  - Jezdić, Zoran
AU  - Aničić, Boban
AU  - Jelovac, Drago
AU  - Jovanović, Svetlana
AU  - Milašin, Jelena
PY  - 2021
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/3283
AB  - Specific cell subpopulations identified as cancer stem cells (CSCs) can be found in basal cell carcinoma (BCC).
Generally, CSCs have a marked trans-differentiation potential that could potentially be used in differentiation therapies.
However, there are no studies regarding BCC CSCs multipotency. The aim of the study was to analyze the characteristic of
CSCs of BCC with emphasis on their differentiation potential upon specific induction. Specific staining and cell
morphology were used for differentiation confirmation, along with the expression analysis of osteogenic (ALP, BSP,
Runx2, OCN, BMP2), chondrogenic (COL1 and COL2A1), adipogenic (PPAR-γ) and neurogenic (Nestin and MAP2)
markers. BCC CSCs differentiated into osteogenic and chondrogenic lineages, as judged by staining and high
expression of specific markers (from 2-to 92-fold higher upon induction). Concomitantly with differentiation, the
levels of cancer stem cell markers decreased in the cultures. Adipo-differentiation and neuro-differentiation were
unsuccessful. In conclusion, BCC CSCs exhibit the capacity to trans-differentiate, a characteristic that may potentially
be useful in the development of new strategies for the treatment of aggressive BCCs.
PB  - TECH SCIENCE PRESS
T2  - BIOCELL
T1  - Basal cell carcinoma stem cells exhibit osteogenic and chondrogenic differentiation potential
VL  - 45
IS  - 6
SP  - 1543
EP  - 1550
DO  - 10.32604/biocell.2021.015060
ER  - 

@article{
author = "Milošević-Marković, Maja and Lazarević, Miloš and Toljić, Boško and Petrović, Milan and Vukadinović, Miroslav and Jezdić, Zoran and Aničić, Boban and Jelovac, Drago and Jovanović, Svetlana and Milašin, Jelena",
year = "2021",
abstract = "Specific cell subpopulations identified as cancer stem cells (CSCs) can be found in basal cell carcinoma (BCC).
Generally, CSCs have a marked trans-differentiation potential that could potentially be used in differentiation therapies.
However, there are no studies regarding BCC CSCs multipotency. The aim of the study was to analyze the characteristic of
CSCs of BCC with emphasis on their differentiation potential upon specific induction. Specific staining and cell
morphology were used for differentiation confirmation, along with the expression analysis of osteogenic (ALP, BSP,
Runx2, OCN, BMP2), chondrogenic (COL1 and COL2A1), adipogenic (PPAR-γ) and neurogenic (Nestin and MAP2)
markers. BCC CSCs differentiated into osteogenic and chondrogenic lineages, as judged by staining and high
expression of specific markers (from 2-to 92-fold higher upon induction). Concomitantly with differentiation, the
levels of cancer stem cell markers decreased in the cultures. Adipo-differentiation and neuro-differentiation were
unsuccessful. In conclusion, BCC CSCs exhibit the capacity to trans-differentiate, a characteristic that may potentially
be useful in the development of new strategies for the treatment of aggressive BCCs.",
publisher = "TECH SCIENCE PRESS",
journal = "BIOCELL",
title = "Basal cell carcinoma stem cells exhibit osteogenic and chondrogenic differentiation potential",
volume = "45",
number = "6",
pages = "1543-1550",
doi = "10.32604/biocell.2021.015060"
}

Milošević-Marković, M., Lazarević, M., Toljić, B., Petrović, M., Vukadinović, M., Jezdić, Z., Aničić, B., Jelovac, D., Jovanović, S.,& Milašin, J.. (2021). Basal cell carcinoma stem cells exhibit osteogenic and chondrogenic differentiation potential. in BIOCELL
TECH SCIENCE PRESS., 45(6), 1543-1550.
https://doi.org/10.32604/biocell.2021.015060

Milošević-Marković M, Lazarević M, Toljić B, Petrović M, Vukadinović M, Jezdić Z, Aničić B, Jelovac D, Jovanović S, Milašin J. Basal cell carcinoma stem cells exhibit osteogenic and chondrogenic differentiation potential. in BIOCELL. 2021;45(6):1543-1550.
doi:10.32604/biocell.2021.015060 .

Milošević-Marković, Maja, Lazarević, Miloš, Toljić, Boško, Petrović, Milan, Vukadinović, Miroslav, Jezdić, Zoran, Aničić, Boban, Jelovac, Drago, Jovanović, Svetlana, Milašin, Jelena, "Basal cell carcinoma stem cells exhibit osteogenic and chondrogenic differentiation potential" in BIOCELL, 45, no. 6 (2021):1543-1550,
https://doi.org/10.32604/biocell.2021.015060 . .

TY  - JOUR
AU  - Dragović, Gordana
AU  - Toljić, Boško
AU  - Jevtović, Đorđe
AU  - Lukić, Relja
AU  - de Luka, Silvio R.
AU  - Trbovich, Alexander
AU  - Milašin, Jelena
PY  - 2021
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/3285
AB  - Background: Components of the metabolic syndrome (MetS) play an important role in the accelerated aging process. Relative telomere length (RTL) is a marker of biological aging. The aim of our study was to determine RTL and its possible association with MetS and the components of MetS in HIV-infected patients treated with cART.

Methods: We included 24 HIV-infected men, all Caucasians, with successful cART (<50 HIV-RNA copies/mL) and on stable cART for at least 24 months. The presence of MetS and its components was determined by the criteria prescribed by the International Diabetes Federation. RTL was determined by Real-Time PCR and ΔΔCt method. We performed a multiple linear regression modeling on log-transformed RTL (dependant variable) to evaluate which components of the metabolic syndrome as well as cART duration and cART type, had an impact on RTL.

Results: Eleven (45.8%) patients had and 13 (54.2%) had not MetS. All patients, had an undetectable viral RNA and a relatively good immune status. The mean RTL was 0.62 ± 0.15 and 0.95 ± 0.36 in patients with and without MetS, respectively (p = 0.01). Multiple linear regression model showed no significant association between duration of cART, cART type and RTL (p = 0.2165, p = 0.8628, respectively). The same analysis showed that an increase in number of MetS components was associated with shorter telomere length (β = -0.4982, p = 0.042).

Conclusions: We showed for the first time association between RTL shortening in HIV-infected men with metabolic syndrome. Furthermore, our study also indicated that an increment of metabolic syndrome components is strongly associated with shorter telomere length.
PB  - Elsevier Inc.
T2  - Experimental Gerontology
T1  - Correlation between metabolic syndrome and relative telomere length shortening in HIV/AIDS patients on combined antiretroviral therapy
VL  - 147
IS  - May
SP  - 111269
DO  - 10.1016/j.exger.2021.111269
ER  - 

@article{
author = "Dragović, Gordana and Toljić, Boško and Jevtović, Đorđe and Lukić, Relja and de Luka, Silvio R. and Trbovich, Alexander and Milašin, Jelena",
year = "2021",
abstract = "Background: Components of the metabolic syndrome (MetS) play an important role in the accelerated aging process. Relative telomere length (RTL) is a marker of biological aging. The aim of our study was to determine RTL and its possible association with MetS and the components of MetS in HIV-infected patients treated with cART.

Methods: We included 24 HIV-infected men, all Caucasians, with successful cART (<50 HIV-RNA copies/mL) and on stable cART for at least 24 months. The presence of MetS and its components was determined by the criteria prescribed by the International Diabetes Federation. RTL was determined by Real-Time PCR and ΔΔCt method. We performed a multiple linear regression modeling on log-transformed RTL (dependant variable) to evaluate which components of the metabolic syndrome as well as cART duration and cART type, had an impact on RTL.

Results: Eleven (45.8%) patients had and 13 (54.2%) had not MetS. All patients, had an undetectable viral RNA and a relatively good immune status. The mean RTL was 0.62 ± 0.15 and 0.95 ± 0.36 in patients with and without MetS, respectively (p = 0.01). Multiple linear regression model showed no significant association between duration of cART, cART type and RTL (p = 0.2165, p = 0.8628, respectively). The same analysis showed that an increase in number of MetS components was associated with shorter telomere length (β = -0.4982, p = 0.042).

Conclusions: We showed for the first time association between RTL shortening in HIV-infected men with metabolic syndrome. Furthermore, our study also indicated that an increment of metabolic syndrome components is strongly associated with shorter telomere length.",
publisher = "Elsevier Inc.",
journal = "Experimental Gerontology",
title = "Correlation between metabolic syndrome and relative telomere length shortening in HIV/AIDS patients on combined antiretroviral therapy",
volume = "147",
number = "May",
pages = "111269",
doi = "10.1016/j.exger.2021.111269"
}

Dragović, G., Toljić, B., Jevtović, Đ., Lukić, R., de Luka, S. R., Trbovich, A.,& Milašin, J.. (2021). Correlation between metabolic syndrome and relative telomere length shortening in HIV/AIDS patients on combined antiretroviral therapy. in Experimental Gerontology
Elsevier Inc.., 147(May), 111269.
https://doi.org/10.1016/j.exger.2021.111269

Dragović G, Toljić B, Jevtović Đ, Lukić R, de Luka SR, Trbovich A, Milašin J. Correlation between metabolic syndrome and relative telomere length shortening in HIV/AIDS patients on combined antiretroviral therapy. in Experimental Gerontology. 2021;147(May):111269.
doi:10.1016/j.exger.2021.111269 .

Dragović, Gordana, Toljić, Boško, Jevtović, Đorđe, Lukić, Relja, de Luka, Silvio R., Trbovich, Alexander, Milašin, Jelena, "Correlation between metabolic syndrome and relative telomere length shortening in HIV/AIDS patients on combined antiretroviral therapy" in Experimental Gerontology, 147, no. May (2021):111269,
https://doi.org/10.1016/j.exger.2021.111269 . .

TY  - JOUR
AU  - Andrić, Miroslav
AU  - Jaćimović, Jelena
AU  - Jakovljević, Aleksandar
AU  - Nikolić, Nadja
AU  - Milašin, Jelena
PY  - 2021
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2578
AB  - Objectives
The aim of this systematic review was to critically analyze available data on gene polymorphisms in odontogenic keratocysts (OKC) and ameloblastomas, including their possible relationship with clinical and histological features of these lesions.

Materials and Methods
A comprehensive search of Web of Science Scopus, PubMed, Cochrane Central Register of Controlled Trials and EMBASE was conducted using relevant key terms and supplemented by a gray literature search. Quality assessment of included studies was performed using criteria from the Strengthening the Reporting of Genetic Association (STREGA) statement.

Results
Ten studies were included in the final review. Survivin ‐31G/C, interleukin IL‐1α ‐889 C/T, p53 codon 72 G/C, tumor necrosis factor TNF‐α (−308G>A) and its receptor TNF‐R1 (36A>G), glioma‐associated oncogene homolog 1 rs2228224 and matrix metalloproteinase 2 rs243865 gene polymorphisms were reported to be associated with OKC. For ameloblastomas, p53 codon 72 G/C, X‐ray repair cross‐complementing protein 1—codons 194 and 399 and matrix metalloproteinase 9 rs3918242 gene polymorphisms were identified as risk factors. It was not possible to establish a relationship between specific polymorphisms and clinical and histological features of investigated lesions.

Conclusions
Several gene polymorphisms might be considered as a risk factor for the development of these lesions. Future studies should investigate whether these polymorphisms might be used to identify patients with increased risk of recurrence or aggressive disease.
PB  - Wiley
T2  - Oral Diseases
T1  - Gene polymorphisms in odontogenic keratocysts and ameloblastomas: A systematic review
DO  - 10.1111/odi.13865
ER  - 

@article{
author = "Andrić, Miroslav and Jaćimović, Jelena and Jakovljević, Aleksandar and Nikolić, Nadja and Milašin, Jelena",
year = "2021",
abstract = "Objectives
The aim of this systematic review was to critically analyze available data on gene polymorphisms in odontogenic keratocysts (OKC) and ameloblastomas, including their possible relationship with clinical and histological features of these lesions.

Materials and Methods
A comprehensive search of Web of Science Scopus, PubMed, Cochrane Central Register of Controlled Trials and EMBASE was conducted using relevant key terms and supplemented by a gray literature search. Quality assessment of included studies was performed using criteria from the Strengthening the Reporting of Genetic Association (STREGA) statement.

Results
Ten studies were included in the final review. Survivin ‐31G/C, interleukin IL‐1α ‐889 C/T, p53 codon 72 G/C, tumor necrosis factor TNF‐α (−308G>A) and its receptor TNF‐R1 (36A>G), glioma‐associated oncogene homolog 1 rs2228224 and matrix metalloproteinase 2 rs243865 gene polymorphisms were reported to be associated with OKC. For ameloblastomas, p53 codon 72 G/C, X‐ray repair cross‐complementing protein 1—codons 194 and 399 and matrix metalloproteinase 9 rs3918242 gene polymorphisms were identified as risk factors. It was not possible to establish a relationship between specific polymorphisms and clinical and histological features of investigated lesions.

Conclusions
Several gene polymorphisms might be considered as a risk factor for the development of these lesions. Future studies should investigate whether these polymorphisms might be used to identify patients with increased risk of recurrence or aggressive disease.",
publisher = "Wiley",
journal = "Oral Diseases",
title = "Gene polymorphisms in odontogenic keratocysts and ameloblastomas: A systematic review",
doi = "10.1111/odi.13865"
}

Andrić, M., Jaćimović, J., Jakovljević, A., Nikolić, N.,& Milašin, J.. (2021). Gene polymorphisms in odontogenic keratocysts and ameloblastomas: A systematic review. in Oral Diseases
Wiley..
https://doi.org/10.1111/odi.13865

Andrić M, Jaćimović J, Jakovljević A, Nikolić N, Milašin J. Gene polymorphisms in odontogenic keratocysts and ameloblastomas: A systematic review. in Oral Diseases. 2021;.
doi:10.1111/odi.13865 .

Andrić, Miroslav, Jaćimović, Jelena, Jakovljević, Aleksandar, Nikolić, Nadja, Milašin, Jelena, "Gene polymorphisms in odontogenic keratocysts and ameloblastomas: A systematic review" in Oral Diseases (2021),
https://doi.org/10.1111/odi.13865 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Nikolić, Nadja
AU  - Jaćimović, Jelena
AU  - Miletić, Maja
AU  - Andrić, Miroslav
AU  - Milašin, Jelena
AU  - Aminoshariae, Anita
AU  - Azarpazhooh, Amir
PY  - 2021
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2577
AB  - Introduction
This study aimed to perform a more precise estimation of the association between tumor necrosis factor-alpha (TNF-α) – 308 G/A single nucleotide polymorphism (SNP) and the risk of development of AP and its phenotypes based on all available published studies.
Methods
The study was carried out according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and is registered in PROSPERO (CRD42020176190). The literature search was conducted via: Clarivate Analytics’ Scopus, PubMed, Cochrane Central Register of Controlled Trials and China National Knowledge Infrastructure databases, from inception to December 2020 with no language restrictions. Two reviewers were involved independently in study selection, data extraction and appraising the studies that were included. The quality of included studies was evaluated using the Strengthening the Reporting of Genetic Association (STREGA) and the Grading of Recommendations Assessment, Development and Evaluation (GRADE) system. Frequencies of genotypes and alleles of TNF-alpha (G>A 308, rs1800629) gene, with 95% Odds ratio was used.
Results
Four studies met the inclusion criteria with moderate risk of bias. This study revealed no significant association between TNF-α – 308 G/A SNP and AP, and the risk of AP development. Moreover, there was no significant association between genotype or allele frequency distribution and clinical manifestations (acute versus chronic) of AP. The certainty of evidence per GRADE was very low.
Conclusions
Due to very low certainty of evidence, whether there is an association between TNF-α – 308 G/A SNP and AP, warrants further well-designed multi-centric studies to adjudicate a better understanding of the role of genetic factors in the etiopathogenesis of AP.
PB  - Elsevier
T2  - Journal of Endodontics
T1  - TNF-α -308 G/A single nucleotide polymorphism and apical periodontitis: an updated systematic review and meta-analysis
DO  - 10.1016/j.joen.2021.03.007
ER  - 

@article{
author = "Jakovljević, Aleksandar and Nikolić, Nadja and Jaćimović, Jelena and Miletić, Maja and Andrić, Miroslav and Milašin, Jelena and Aminoshariae, Anita and Azarpazhooh, Amir",
year = "2021",
abstract = "Introduction
This study aimed to perform a more precise estimation of the association between tumor necrosis factor-alpha (TNF-α) – 308 G/A single nucleotide polymorphism (SNP) and the risk of development of AP and its phenotypes based on all available published studies.
Methods
The study was carried out according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and is registered in PROSPERO (CRD42020176190). The literature search was conducted via: Clarivate Analytics’ Scopus, PubMed, Cochrane Central Register of Controlled Trials and China National Knowledge Infrastructure databases, from inception to December 2020 with no language restrictions. Two reviewers were involved independently in study selection, data extraction and appraising the studies that were included. The quality of included studies was evaluated using the Strengthening the Reporting of Genetic Association (STREGA) and the Grading of Recommendations Assessment, Development and Evaluation (GRADE) system. Frequencies of genotypes and alleles of TNF-alpha (G>A 308, rs1800629) gene, with 95% Odds ratio was used.
Results
Four studies met the inclusion criteria with moderate risk of bias. This study revealed no significant association between TNF-α – 308 G/A SNP and AP, and the risk of AP development. Moreover, there was no significant association between genotype or allele frequency distribution and clinical manifestations (acute versus chronic) of AP. The certainty of evidence per GRADE was very low.
Conclusions
Due to very low certainty of evidence, whether there is an association between TNF-α – 308 G/A SNP and AP, warrants further well-designed multi-centric studies to adjudicate a better understanding of the role of genetic factors in the etiopathogenesis of AP.",
publisher = "Elsevier",
journal = "Journal of Endodontics",
title = "TNF-α -308 G/A single nucleotide polymorphism and apical periodontitis: an updated systematic review and meta-analysis",
doi = "10.1016/j.joen.2021.03.007"
}

Jakovljević, A., Nikolić, N., Jaćimović, J., Miletić, M., Andrić, M., Milašin, J., Aminoshariae, A.,& Azarpazhooh, A.. (2021). TNF-α -308 G/A single nucleotide polymorphism and apical periodontitis: an updated systematic review and meta-analysis. in Journal of Endodontics
Elsevier..
https://doi.org/10.1016/j.joen.2021.03.007

Jakovljević A, Nikolić N, Jaćimović J, Miletić M, Andrić M, Milašin J, Aminoshariae A, Azarpazhooh A. TNF-α -308 G/A single nucleotide polymorphism and apical periodontitis: an updated systematic review and meta-analysis. in Journal of Endodontics. 2021;.
doi:10.1016/j.joen.2021.03.007 .

Jakovljević, Aleksandar, Nikolić, Nadja, Jaćimović, Jelena, Miletić, Maja, Andrić, Miroslav, Milašin, Jelena, Aminoshariae, Anita, Azarpazhooh, Amir, "TNF-α -308 G/A single nucleotide polymorphism and apical periodontitis: an updated systematic review and meta-analysis" in Journal of Endodontics (2021),
https://doi.org/10.1016/j.joen.2021.03.007 . .

TY  - JOUR
AU  - Đinić Krasavčević, Ana
AU  - Nikolić, Nadja
AU  - Mijailović, Iva
AU  - Čarkić, Jelena
AU  - Milinković, Iva
AU  - Janković, Saša
AU  - Aleksić, Zoran
AU  - Milašin, Jelena
PY  - 2021
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2568
AB  - Background and objective
Notch signalling cascade has recently been connected to alveolar bone resorption in periodontitis. Hence, the present cross‐sectional study aimed to analyze the expression of Notch signalling pathway (Notch 1, Notch 2, Jagged 1, Hes 1, Hey 1) and periodontitis‐related (tumor necrosis factor alpha‐ TNF‐α, interleukin 17‐IL‐17, receptor activator of nuclear factor‐kappa B ligand—RANKL, osteoprotegerin—OPG) molecules and correlate it with clinical parameters in aggressive (AP) and chronic (CP) periodontitis. Additionally, the aforementioned markers' expression was evaluated in periodontitis patients with different RANKL/OPG ratios.

Material and methods
Eighty patients were enrolled either in AP or CP group. Clinical attachment level (CAL), bleeding on probing (BOP), periodontal probing depth (PPD) and plaque index (PI) were recorded for each patient. Total RNA was extracted from gingival crevicular fluid samples. Relative gene expression of investigated markers was determined by reverse transcriptase‐real‐time polymerase chain reaction.

Results
Significantly higher values of PPD were observed in AP compared to CP (P = .010). Negative correlations between OPG and CAL, and OPG and PI, were found in AP (P = .045, P = .006, respectively), while Hey 1 and PI had a positive correlation (P = .049). In multivariate linear regression analysis, OPG and Notch 2 were predictors of CAL in AP group. TNF‐α and IL‐17 were higher in RANKL predominant than in OPG predominant cases (P = .007, P = .001, respectively). In RANKL predominant lesions Notch 1 and Jagged 1 were down‐regulated in AP compared to CP patients (P = .010, P = .025, respectively).

Conclusion
The present study demonstrated that changes in Notch 2 expression affected CAL in AP cases hence this molecule could be considered as a contributor to alveolar bone loss. In RANKL‐activated settings, the down‐regulation of Notch 1 might participate in more severe bone resorption in AP.
PB  - Wiley
T2  - Journal of Periodontal Research
T1  - Impact of Notch signalling molecules and bone resorption regulators on clinical parameters in periodontitis
VL  - 56
IS  - 1
SP  - 131
EP  - 138
DO  - 10.1111/jre.12801
ER  - 

@article{
author = "Đinić Krasavčević, Ana and Nikolić, Nadja and Mijailović, Iva and Čarkić, Jelena and Milinković, Iva and Janković, Saša and Aleksić, Zoran and Milašin, Jelena",
year = "2021",
abstract = "Background and objective
Notch signalling cascade has recently been connected to alveolar bone resorption in periodontitis. Hence, the present cross‐sectional study aimed to analyze the expression of Notch signalling pathway (Notch 1, Notch 2, Jagged 1, Hes 1, Hey 1) and periodontitis‐related (tumor necrosis factor alpha‐ TNF‐α, interleukin 17‐IL‐17, receptor activator of nuclear factor‐kappa B ligand—RANKL, osteoprotegerin—OPG) molecules and correlate it with clinical parameters in aggressive (AP) and chronic (CP) periodontitis. Additionally, the aforementioned markers' expression was evaluated in periodontitis patients with different RANKL/OPG ratios.

Material and methods
Eighty patients were enrolled either in AP or CP group. Clinical attachment level (CAL), bleeding on probing (BOP), periodontal probing depth (PPD) and plaque index (PI) were recorded for each patient. Total RNA was extracted from gingival crevicular fluid samples. Relative gene expression of investigated markers was determined by reverse transcriptase‐real‐time polymerase chain reaction.

Results
Significantly higher values of PPD were observed in AP compared to CP (P = .010). Negative correlations between OPG and CAL, and OPG and PI, were found in AP (P = .045, P = .006, respectively), while Hey 1 and PI had a positive correlation (P = .049). In multivariate linear regression analysis, OPG and Notch 2 were predictors of CAL in AP group. TNF‐α and IL‐17 were higher in RANKL predominant than in OPG predominant cases (P = .007, P = .001, respectively). In RANKL predominant lesions Notch 1 and Jagged 1 were down‐regulated in AP compared to CP patients (P = .010, P = .025, respectively).

Conclusion
The present study demonstrated that changes in Notch 2 expression affected CAL in AP cases hence this molecule could be considered as a contributor to alveolar bone loss. In RANKL‐activated settings, the down‐regulation of Notch 1 might participate in more severe bone resorption in AP.",
publisher = "Wiley",
journal = "Journal of Periodontal Research",
title = "Impact of Notch signalling molecules and bone resorption regulators on clinical parameters in periodontitis",
volume = "56",
number = "1",
pages = "131-138",
doi = "10.1111/jre.12801"
}

Đinić Krasavčević, A., Nikolić, N., Mijailović, I., Čarkić, J., Milinković, I., Janković, S., Aleksić, Z.,& Milašin, J.. (2021). Impact of Notch signalling molecules and bone resorption regulators on clinical parameters in periodontitis. in Journal of Periodontal Research
Wiley., 56(1), 131-138.
https://doi.org/10.1111/jre.12801

Đinić Krasavčević A, Nikolić N, Mijailović I, Čarkić J, Milinković I, Janković S, Aleksić Z, Milašin J. Impact of Notch signalling molecules and bone resorption regulators on clinical parameters in periodontitis. in Journal of Periodontal Research. 2021;56(1):131-138.
doi:10.1111/jre.12801 .

Đinić Krasavčević, Ana, Nikolić, Nadja, Mijailović, Iva, Čarkić, Jelena, Milinković, Iva, Janković, Saša, Aleksić, Zoran, Milašin, Jelena, "Impact of Notch signalling molecules and bone resorption regulators on clinical parameters in periodontitis" in Journal of Periodontal Research, 56, no. 1 (2021):131-138,
https://doi.org/10.1111/jre.12801 . .

TY  - JOUR
AU  - Dragović, Miroslav
AU  - Pejović, Marko
AU  - Stepić, Jelena
AU  - Čolić, Snježana
AU  - Dožić, Branko
AU  - Dragović, Svetlana
AU  - Lazarević, Miloš
AU  - Nikolić, Nadja
AU  - Milašin, Jelena
AU  - Miličić, Biljana
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2513
AB  - Objectives Sutures are the most frequently used medical device for wound closure. They support tissue during the early phase of healing until it regains enough tensile strength. The aim of this study was to compare four different suture materials in terms of the influence on wound healing, microbial adherence, tissue reaction, and relevant clinical parameters which determine their clinical value. Materials and Methods Total number of 32 patients undergoing surgical extraction of four impacted third molars were involved in the study. Clinical parameters were estimated intraoperatively and during the control check-ups. Soft tissue healing around sutures were evaluated on the 3rd and 7th day postoperatively. Microbial colonization was assessed by means of qPCR. Also, histological analysis was done to assess inflammatory reaction. Results Significantly better soft tissue healing was found around monofilament and synthetic sutures compared to multifilament and natural ones respectively. Soft tissue healing was significantly better around all sutures on the 7th day than on the 3rd day postoperatively. Conclusions Non-resorbable polypropylene suture showed superior clinical characteristics among all sutures. Moreover, the best healing of soft tissue and the least inflammatory reaction was found around this thread. The poorest soft tissue healing was found around non-resorbable silk suture. This suture elicited strongest inflammatory reaction and showed the greatest microbial adherence affinity compared to alternative sutures.
PB  - Springer Heidelberg, Heidelberg
T2  - Clinical Oral Investigations
T1  - Comparison of four different suture materials in respect to oral wound healing, microbial colonization, tissue reaction and clinical features-randomized clinical study
VL  - 24
IS  - 4
SP  - 1527
EP  - 1541
DO  - 10.1007/s00784-019-03034-4
ER  - 

@article{
author = "Dragović, Miroslav and Pejović, Marko and Stepić, Jelena and Čolić, Snježana and Dožić, Branko and Dragović, Svetlana and Lazarević, Miloš and Nikolić, Nadja and Milašin, Jelena and Miličić, Biljana",
year = "2020",
abstract = "Objectives Sutures are the most frequently used medical device for wound closure. They support tissue during the early phase of healing until it regains enough tensile strength. The aim of this study was to compare four different suture materials in terms of the influence on wound healing, microbial adherence, tissue reaction, and relevant clinical parameters which determine their clinical value. Materials and Methods Total number of 32 patients undergoing surgical extraction of four impacted third molars were involved in the study. Clinical parameters were estimated intraoperatively and during the control check-ups. Soft tissue healing around sutures were evaluated on the 3rd and 7th day postoperatively. Microbial colonization was assessed by means of qPCR. Also, histological analysis was done to assess inflammatory reaction. Results Significantly better soft tissue healing was found around monofilament and synthetic sutures compared to multifilament and natural ones respectively. Soft tissue healing was significantly better around all sutures on the 7th day than on the 3rd day postoperatively. Conclusions Non-resorbable polypropylene suture showed superior clinical characteristics among all sutures. Moreover, the best healing of soft tissue and the least inflammatory reaction was found around this thread. The poorest soft tissue healing was found around non-resorbable silk suture. This suture elicited strongest inflammatory reaction and showed the greatest microbial adherence affinity compared to alternative sutures.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Clinical Oral Investigations",
title = "Comparison of four different suture materials in respect to oral wound healing, microbial colonization, tissue reaction and clinical features-randomized clinical study",
volume = "24",
number = "4",
pages = "1527-1541",
doi = "10.1007/s00784-019-03034-4"
}

Dragović, M., Pejović, M., Stepić, J., Čolić, S., Dožić, B., Dragović, S., Lazarević, M., Nikolić, N., Milašin, J.,& Miličić, B.. (2020). Comparison of four different suture materials in respect to oral wound healing, microbial colonization, tissue reaction and clinical features-randomized clinical study. in Clinical Oral Investigations
Springer Heidelberg, Heidelberg., 24(4), 1527-1541.
https://doi.org/10.1007/s00784-019-03034-4

Dragović M, Pejović M, Stepić J, Čolić S, Dožić B, Dragović S, Lazarević M, Nikolić N, Milašin J, Miličić B. Comparison of four different suture materials in respect to oral wound healing, microbial colonization, tissue reaction and clinical features-randomized clinical study. in Clinical Oral Investigations. 2020;24(4):1527-1541.
doi:10.1007/s00784-019-03034-4 .

Dragović, Miroslav, Pejović, Marko, Stepić, Jelena, Čolić, Snježana, Dožić, Branko, Dragović, Svetlana, Lazarević, Miloš, Nikolić, Nadja, Milašin, Jelena, Miličić, Biljana, "Comparison of four different suture materials in respect to oral wound healing, microbial colonization, tissue reaction and clinical features-randomized clinical study" in Clinical Oral Investigations, 24, no. 4 (2020):1527-1541,
https://doi.org/10.1007/s00784-019-03034-4 . .

TY  - JOUR
AU  - Čarkić, Jelena
AU  - Nikolić, Nadja
AU  - Nišević, Jelena
AU  - Lazarević, Miloš
AU  - Kuzmanović-Pfićer, Jovana
AU  - Jelovac, Drago
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2567
AB  - Oral carcinoma is the sixth most common malignancy worldwide, with survival rates of approximately 50%. The major type of oral cancer, present in 90% of the cases, is oral squamous cell carcinoma (OSCC). The genetic background predisposing an individual to OSCC is complex and largely unknown. Studies have suggested that endothelial nitric oxide synthase (eNOS) gene polymorphisms modulate the cancer risk, prompting us to assess the impact of three functional eNOS gene polymorphisms on OSCC risk. The present study included 50 patients with OSCC and 110 controls. Polymerase chain reaction and restriction fragment length polymorphism analysis were used for genotyping of single-nucleotide polymorphisms -786 T/C (rs2070744) and 894 G/T (rs1799983) and variable number of tandem repeats (VNTR) intron 4b/a polymorphism. Homozygous carriers of -786 T/C and intron 4b/a VNTR variant alleles paired with a significant increase of oral cancer risk [odds ratio (OR): 3.63, 95% confidence interval (CI): 1.08-12.21; P = 0.045 and OR: 11.29, 95% CI: 2.71-47.11; P < 0.001, respectively]. When combined, CC and 4b4a genotypes together led to a 21-fold OSCC risk increase (OR: 21, 95% CI: 2.07-213.29; P = 0.006). Haplotype analysis showed that the C-G-4b haplotype conferred an 11-fold increase in OSCC risk. In conclusion, eNOS polymorphisms considerably influence levels of OSCC risk in the Serbian population.
PB  - Nihon University, School of Dentistry, Japan
T2  - Journal of Oral Science
T1  - Endothelial nitric oxide synthase polymorphisms/haplotypes are strong modulators of oral cancer risk in Serbian population
VL  - 62
IS  - 3
SP  - 322
EP  - 326
DO  - 10.2334/josnusd.19-0310
ER  - 

@article{
author = "Čarkić, Jelena and Nikolić, Nadja and Nišević, Jelena and Lazarević, Miloš and Kuzmanović-Pfićer, Jovana and Jelovac, Drago and Milašin, Jelena",
year = "2020",
abstract = "Oral carcinoma is the sixth most common malignancy worldwide, with survival rates of approximately 50%. The major type of oral cancer, present in 90% of the cases, is oral squamous cell carcinoma (OSCC). The genetic background predisposing an individual to OSCC is complex and largely unknown. Studies have suggested that endothelial nitric oxide synthase (eNOS) gene polymorphisms modulate the cancer risk, prompting us to assess the impact of three functional eNOS gene polymorphisms on OSCC risk. The present study included 50 patients with OSCC and 110 controls. Polymerase chain reaction and restriction fragment length polymorphism analysis were used for genotyping of single-nucleotide polymorphisms -786 T/C (rs2070744) and 894 G/T (rs1799983) and variable number of tandem repeats (VNTR) intron 4b/a polymorphism. Homozygous carriers of -786 T/C and intron 4b/a VNTR variant alleles paired with a significant increase of oral cancer risk [odds ratio (OR): 3.63, 95% confidence interval (CI): 1.08-12.21; P = 0.045 and OR: 11.29, 95% CI: 2.71-47.11; P < 0.001, respectively]. When combined, CC and 4b4a genotypes together led to a 21-fold OSCC risk increase (OR: 21, 95% CI: 2.07-213.29; P = 0.006). Haplotype analysis showed that the C-G-4b haplotype conferred an 11-fold increase in OSCC risk. In conclusion, eNOS polymorphisms considerably influence levels of OSCC risk in the Serbian population.",
publisher = "Nihon University, School of Dentistry, Japan",
journal = "Journal of Oral Science",
title = "Endothelial nitric oxide synthase polymorphisms/haplotypes are strong modulators of oral cancer risk in Serbian population",
volume = "62",
number = "3",
pages = "322-326",
doi = "10.2334/josnusd.19-0310"
}

Čarkić, J., Nikolić, N., Nišević, J., Lazarević, M., Kuzmanović-Pfićer, J., Jelovac, D.,& Milašin, J.. (2020). Endothelial nitric oxide synthase polymorphisms/haplotypes are strong modulators of oral cancer risk in Serbian population. in Journal of Oral Science
Nihon University, School of Dentistry, Japan., 62(3), 322-326.
https://doi.org/10.2334/josnusd.19-0310

Čarkić J, Nikolić N, Nišević J, Lazarević M, Kuzmanović-Pfićer J, Jelovac D, Milašin J. Endothelial nitric oxide synthase polymorphisms/haplotypes are strong modulators of oral cancer risk in Serbian population. in Journal of Oral Science. 2020;62(3):322-326.
doi:10.2334/josnusd.19-0310 .

Čarkić, Jelena, Nikolić, Nadja, Nišević, Jelena, Lazarević, Miloš, Kuzmanović-Pfićer, Jovana, Jelovac, Drago, Milašin, Jelena, "Endothelial nitric oxide synthase polymorphisms/haplotypes are strong modulators of oral cancer risk in Serbian population" in Journal of Oral Science, 62, no. 3 (2020):322-326,
https://doi.org/10.2334/josnusd.19-0310 . .

TY  - JOUR
AU  - Lazarević, Miloš
AU  - Milošević, Maja
AU  - Jelovac, Drago
AU  - Milenković, Sanja
AU  - Tepavčević, Zvezdana
AU  - Baldan, Federica
AU  - Suboticki, Tijana
AU  - Toljić, Boško
AU  - Trišić, Dijana
AU  - Dragović, Miroslav
AU  - Damante, Giuseppe
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2484
AB  - Epithelial to mesenchymal transition (EMT) is a feature of several types of human cancer, including oral squamous cell carcinoma (OSCC). In the present study, tumor and margin cell cultures obtained from patients with OSCC were used to determine the expression patterns of certain EMT-associated markers, including vimentin, alpha -smooth muscle actin, SLUG and SNAIL. In addition, other EMT-associated features, including clonal, proliferative and migratory potential were compared between the two cell types. Cell cultures were generated from tumor and margin tissue samples from 6 patients and cultured up to the fifth passage. EMT marker expression was assessed by reverse transcription-quantitative PCR. Cell proliferation, colony formation and scratch wound healing assays were conducted to characterize the two cell types in terms of proliferation rates, clonality and motility. All of the studied markers were expressed in tumor and margin cells. Although no significant differences were noted with regard to the aforementioned markers, their expression tended to be higher in margin cultures than in tumor cultures. The expressions of the EMT markers were also higher in the fifth passage compared with those noted at the first with a few exceptions. The rates of proliferation and cell migration were decreased during passages, while the number of colonies was increased in both types of cell culture. Tumor and margin cells indicated certain similarities with regard to EMT transition characteristics.
PB  - SPANDIDOS PUBL LTD, ATHENS
T2  - Oncology Letters
T1  - Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study
VL  - 19
IS  - 6
SP  - 3743
EP  - 3750
DO  - 10.3892/ol.2020.11494
ER  - 

@article{
author = "Lazarević, Miloš and Milošević, Maja and Jelovac, Drago and Milenković, Sanja and Tepavčević, Zvezdana and Baldan, Federica and Suboticki, Tijana and Toljić, Boško and Trišić, Dijana and Dragović, Miroslav and Damante, Giuseppe and Milašin, Jelena",
year = "2020",
abstract = "Epithelial to mesenchymal transition (EMT) is a feature of several types of human cancer, including oral squamous cell carcinoma (OSCC). In the present study, tumor and margin cell cultures obtained from patients with OSCC were used to determine the expression patterns of certain EMT-associated markers, including vimentin, alpha -smooth muscle actin, SLUG and SNAIL. In addition, other EMT-associated features, including clonal, proliferative and migratory potential were compared between the two cell types. Cell cultures were generated from tumor and margin tissue samples from 6 patients and cultured up to the fifth passage. EMT marker expression was assessed by reverse transcription-quantitative PCR. Cell proliferation, colony formation and scratch wound healing assays were conducted to characterize the two cell types in terms of proliferation rates, clonality and motility. All of the studied markers were expressed in tumor and margin cells. Although no significant differences were noted with regard to the aforementioned markers, their expression tended to be higher in margin cultures than in tumor cultures. The expressions of the EMT markers were also higher in the fifth passage compared with those noted at the first with a few exceptions. The rates of proliferation and cell migration were decreased during passages, while the number of colonies was increased in both types of cell culture. Tumor and margin cells indicated certain similarities with regard to EMT transition characteristics.",
publisher = "SPANDIDOS PUBL LTD, ATHENS",
journal = "Oncology Letters",
title = "Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study",
volume = "19",
number = "6",
pages = "3743-3750",
doi = "10.3892/ol.2020.11494"
}

Lazarević, M., Milošević, M., Jelovac, D., Milenković, S., Tepavčević, Z., Baldan, F., Suboticki, T., Toljić, B., Trišić, D., Dragović, M., Damante, G.,& Milašin, J.. (2020). Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study. in Oncology Letters
SPANDIDOS PUBL LTD, ATHENS., 19(6), 3743-3750.
https://doi.org/10.3892/ol.2020.11494

Lazarević M, Milošević M, Jelovac D, Milenković S, Tepavčević Z, Baldan F, Suboticki T, Toljić B, Trišić D, Dragović M, Damante G, Milašin J. Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study. in Oncology Letters. 2020;19(6):3743-3750.
doi:10.3892/ol.2020.11494 .

Lazarević, Miloš, Milošević, Maja, Jelovac, Drago, Milenković, Sanja, Tepavčević, Zvezdana, Baldan, Federica, Suboticki, Tijana, Toljić, Boško, Trišić, Dijana, Dragović, Miroslav, Damante, Giuseppe, Milašin, Jelena, "Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study" in Oncology Letters, 19, no. 6 (2020):3743-3750,
https://doi.org/10.3892/ol.2020.11494 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2581
AB  - Article Title and Bibliographic Information: Association between human cytomegalovirus and periodontitis: A systematic review and meta-analysis. Botero JE, Rodríguez-Medina C, Jaramillo-Echeverry A, Contreras A. J Periodontal Res. 2020;55(4):551-558.
Subjects or Study Selection The authors searched the literature, published in English, Spanish, and Portuguese, from inception to May 2019. It was searched using the Medical Subject Heading (MeSH) terms and keywords: (((human cytomegalovirus [Title/Abstract]) OR HCMV [Title/Abstract] OR herpesvirus [Title/Abstract]) AND periodontitis [Title/Abstract] AND (Humans [Mesh])) via MEDLINE, Scopus, and SciELO databases. The authors did not report the use of additional literature sources (eg, citation mining and/or gray literature). Two reviewers independently screened titles and abstracts to identify relevant articles, and a third reviewer was consulted in case of disagreement. Based on eligibility criteria, 32 studies were included for the qualitative and quantitative analyses, 26 on periodontitis and 6 on patients with apical periodontitis. Key Study Factor The authors conducted a systematic review and meta-analysis of case-control and cross-sectional studies on the associations of human cytomegalovirus (HCMV) with periodontitis, and with apical periodontitis of endodontic origin. Studies included populations consisted of periodontal and endodontic patients positive for the presence of HCMV in examined samples compared with patients with negative HCMV result. Main Outcome Measure The main outcome measure was the prevalence of HCMV infection analyzed by different laboratory methods (ie, variants of polymerase chain reaction [PCR] methods [single, nested, multiplex, hot starts, real-time], and loop-mediated isothermal amplification) in periodontitis and apical periodontitis samples compared with healthy controls. In addition, the authors performed quality assessment analyses of the included studies based on their risk of publication bias. Main Results The pooled analysis of 25 studies with 2385 subjects (1324 with periodontitis and 1061 without periodontitis) revealed significantly increased odds for periodontitis when subgingival HCMV was detected (odds ratio [OR] = 5.31; 95% confidence interval [CI] = 3.15-8.97; P < .00001). Heterogeneity test was 1.00, and the test for overall effect was Z = 6.26, which indicates a significant heterogeneity (I-2 67%; P < .00001). In contrast, results from 5 included studies (n = 317, 219 periapical lesions and 98 healthy control samples) revealed no significant odds for apical periodontitis when HCMV was detected (OR = 3.65; 95% CI = 0.49-27.1; P = .21). Heterogeneity test was 3.94, and the test for overall effect was Z = 1.26 (I-2 83%; P < .00001). The quality of the included studies was assessed by analyzing their risk of bias using the US National Institute of Health National Heart, Lung and Blood Institute quality assessment tool for case-control, cohort, and cross-sectional studies. Of 25 studies with periodontitis samples, 18 were considered to be of fair quality, 7 were of poor quality, and only one study was appraised as good quality. Of 6 studies with apical periodontitis samples, 2 were considered to be of fair quality and 4 were of poor quality. Significant publication bias was observed among the 25 included studies related to periodontitis (Egger's test t = 7.0479, df = 23, P < .0001). Conclusions HCMV is significantly more common in patients with periodontitis than in healthy control samples. However, there was no significant difference in the occurrence of HCMV in patients with apical periodontitis compared with healthy control samples.
PB  - Elsevier
T2  - Journal of Evidence Based Dental Practice
T1  - Human Cytomegalovirus May Contribute to the Development of Periodontitis, but Not to Apical Periodontitis of Endodontic Origin
VL  - 20
IS  - 4
SP  - 101491
DO  - 10.1016/j.jebdp.2020.101491
ER  - 

@article{
author = "Jakovljević, Aleksandar and Milašin, Jelena",
year = "2020",
abstract = "Article Title and Bibliographic Information: Association between human cytomegalovirus and periodontitis: A systematic review and meta-analysis. Botero JE, Rodríguez-Medina C, Jaramillo-Echeverry A, Contreras A. J Periodontal Res. 2020;55(4):551-558.
Subjects or Study Selection The authors searched the literature, published in English, Spanish, and Portuguese, from inception to May 2019. It was searched using the Medical Subject Heading (MeSH) terms and keywords: (((human cytomegalovirus [Title/Abstract]) OR HCMV [Title/Abstract] OR herpesvirus [Title/Abstract]) AND periodontitis [Title/Abstract] AND (Humans [Mesh])) via MEDLINE, Scopus, and SciELO databases. The authors did not report the use of additional literature sources (eg, citation mining and/or gray literature). Two reviewers independently screened titles and abstracts to identify relevant articles, and a third reviewer was consulted in case of disagreement. Based on eligibility criteria, 32 studies were included for the qualitative and quantitative analyses, 26 on periodontitis and 6 on patients with apical periodontitis. Key Study Factor The authors conducted a systematic review and meta-analysis of case-control and cross-sectional studies on the associations of human cytomegalovirus (HCMV) with periodontitis, and with apical periodontitis of endodontic origin. Studies included populations consisted of periodontal and endodontic patients positive for the presence of HCMV in examined samples compared with patients with negative HCMV result. Main Outcome Measure The main outcome measure was the prevalence of HCMV infection analyzed by different laboratory methods (ie, variants of polymerase chain reaction [PCR] methods [single, nested, multiplex, hot starts, real-time], and loop-mediated isothermal amplification) in periodontitis and apical periodontitis samples compared with healthy controls. In addition, the authors performed quality assessment analyses of the included studies based on their risk of publication bias. Main Results The pooled analysis of 25 studies with 2385 subjects (1324 with periodontitis and 1061 without periodontitis) revealed significantly increased odds for periodontitis when subgingival HCMV was detected (odds ratio [OR] = 5.31; 95% confidence interval [CI] = 3.15-8.97; P < .00001). Heterogeneity test was 1.00, and the test for overall effect was Z = 6.26, which indicates a significant heterogeneity (I-2 67%; P < .00001). In contrast, results from 5 included studies (n = 317, 219 periapical lesions and 98 healthy control samples) revealed no significant odds for apical periodontitis when HCMV was detected (OR = 3.65; 95% CI = 0.49-27.1; P = .21). Heterogeneity test was 3.94, and the test for overall effect was Z = 1.26 (I-2 83%; P < .00001). The quality of the included studies was assessed by analyzing their risk of bias using the US National Institute of Health National Heart, Lung and Blood Institute quality assessment tool for case-control, cohort, and cross-sectional studies. Of 25 studies with periodontitis samples, 18 were considered to be of fair quality, 7 were of poor quality, and only one study was appraised as good quality. Of 6 studies with apical periodontitis samples, 2 were considered to be of fair quality and 4 were of poor quality. Significant publication bias was observed among the 25 included studies related to periodontitis (Egger's test t = 7.0479, df = 23, P < .0001). Conclusions HCMV is significantly more common in patients with periodontitis than in healthy control samples. However, there was no significant difference in the occurrence of HCMV in patients with apical periodontitis compared with healthy control samples.",
publisher = "Elsevier",
journal = "Journal of Evidence Based Dental Practice",
title = "Human Cytomegalovirus May Contribute to the Development of Periodontitis, but Not to Apical Periodontitis of Endodontic Origin",
volume = "20",
number = "4",
pages = "101491",
doi = "10.1016/j.jebdp.2020.101491"
}

Jakovljević, A.,& Milašin, J.. (2020). Human Cytomegalovirus May Contribute to the Development of Periodontitis, but Not to Apical Periodontitis of Endodontic Origin. in Journal of Evidence Based Dental Practice
Elsevier., 20(4), 101491.
https://doi.org/10.1016/j.jebdp.2020.101491

Jakovljević A, Milašin J. Human Cytomegalovirus May Contribute to the Development of Periodontitis, but Not to Apical Periodontitis of Endodontic Origin. in Journal of Evidence Based Dental Practice. 2020;20(4):101491.
doi:10.1016/j.jebdp.2020.101491 .

Jakovljević, Aleksandar, Milašin, Jelena, "Human Cytomegalovirus May Contribute to the Development of Periodontitis, but Not to Apical Periodontitis of Endodontic Origin" in Journal of Evidence Based Dental Practice, 20, no. 4 (2020):101491,
https://doi.org/10.1016/j.jebdp.2020.101491 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Duncan, Henry F.
AU  - Nagendrababu, Venkateshbabu
AU  - Jaćimović, Jelena
AU  - Milašin, Jelena
AU  - Dummer, Paul
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2573
AB  - Background: The existence of an association between cardiovascular diseases (CVDs) and apical periodontitis (AP) remains unclear because results obtained from previous clinical studies and reviews are inconsistent or inconclusive.

Objective: To conduct an umbrella review to determine whether there is an association between CVDs and the prevalence of AP in adults.

Methods: The protocol of the review was registered in the PROSPERO database (CRD42020185753). The literature search was conducted using the following electronic databases: Clarivate Analytics' Web of Science Scopus, PubMed and Cochrane Database of Systematic Reviews, from inception to May, 2020, with no language restrictions. Systematic reviews with or without meta-analysis that evaluated the association between CVDs and AP were included. Other types of studies, including narrative reviews, were excluded. Two reviewers independently performed a literature search, data extraction and quality assessment of included studies. Any disagreements or doubts were resolved by a third reviewer. The quality of the reviews was assessed using the AMSTAR 2 tool (A measurement tool to assess systematic reviews), with 16 items. A final categorization of the systematic reviews classified each as of 'high', 'moderate', 'low' or 'critically low' quality.

Results: Four systematic reviews were included in the current review. Three reviews were graded by AMSTAR 2 as 'moderate' quality, whereas one review was graded as 'critically low' quality.

Discussion: Only one systematic review included a meta-analysis. Substantial heterogeneity amongst the primary studies included within each systematic review was notable in preventing a pooled analysis.

Conclusions: From the limited 'moderate' to 'critically low' quality evidence available, the current umbrella review concluded that a weak association exists between CVDs and AP. In the future, well-designed, longitudinal clinical studies with long-term follow-up are required.
PB  - Wiley
T2  - International Endodontic Journal
T1  - Association between cardiovascular diseases and apical periodontitis: an umbrella review
VL  - 53
IS  - 10
SP  - 1374
EP  - 1386
DO  - 10.1111/iej.13364
ER  - 

@article{
author = "Jakovljević, Aleksandar and Duncan, Henry F. and Nagendrababu, Venkateshbabu and Jaćimović, Jelena and Milašin, Jelena and Dummer, Paul",
year = "2020",
abstract = "Background: The existence of an association between cardiovascular diseases (CVDs) and apical periodontitis (AP) remains unclear because results obtained from previous clinical studies and reviews are inconsistent or inconclusive.

Objective: To conduct an umbrella review to determine whether there is an association between CVDs and the prevalence of AP in adults.

Methods: The protocol of the review was registered in the PROSPERO database (CRD42020185753). The literature search was conducted using the following electronic databases: Clarivate Analytics' Web of Science Scopus, PubMed and Cochrane Database of Systematic Reviews, from inception to May, 2020, with no language restrictions. Systematic reviews with or without meta-analysis that evaluated the association between CVDs and AP were included. Other types of studies, including narrative reviews, were excluded. Two reviewers independently performed a literature search, data extraction and quality assessment of included studies. Any disagreements or doubts were resolved by a third reviewer. The quality of the reviews was assessed using the AMSTAR 2 tool (A measurement tool to assess systematic reviews), with 16 items. A final categorization of the systematic reviews classified each as of 'high', 'moderate', 'low' or 'critically low' quality.

Results: Four systematic reviews were included in the current review. Three reviews were graded by AMSTAR 2 as 'moderate' quality, whereas one review was graded as 'critically low' quality.

Discussion: Only one systematic review included a meta-analysis. Substantial heterogeneity amongst the primary studies included within each systematic review was notable in preventing a pooled analysis.

Conclusions: From the limited 'moderate' to 'critically low' quality evidence available, the current umbrella review concluded that a weak association exists between CVDs and AP. In the future, well-designed, longitudinal clinical studies with long-term follow-up are required.",
publisher = "Wiley",
journal = "International Endodontic Journal",
title = "Association between cardiovascular diseases and apical periodontitis: an umbrella review",
volume = "53",
number = "10",
pages = "1374-1386",
doi = "10.1111/iej.13364"
}

Jakovljević, A., Duncan, H. F., Nagendrababu, V., Jaćimović, J., Milašin, J.,& Dummer, P.. (2020). Association between cardiovascular diseases and apical periodontitis: an umbrella review. in International Endodontic Journal
Wiley., 53(10), 1374-1386.
https://doi.org/10.1111/iej.13364

Jakovljević A, Duncan HF, Nagendrababu V, Jaćimović J, Milašin J, Dummer P. Association between cardiovascular diseases and apical periodontitis: an umbrella review. in International Endodontic Journal. 2020;53(10):1374-1386.
doi:10.1111/iej.13364 .

Jakovljević, Aleksandar, Duncan, Henry F., Nagendrababu, Venkateshbabu, Jaćimović, Jelena, Milašin, Jelena, Dummer, Paul, "Association between cardiovascular diseases and apical periodontitis: an umbrella review" in International Endodontic Journal, 53, no. 10 (2020):1374-1386,
https://doi.org/10.1111/iej.13364 . .

TY  - JOUR
AU  - Šljivančanin Jakovljević, Tamara
AU  - Kontić-Vučinić, Olivera
AU  - Nikolić, Nadja
AU  - Čarkić, Jelena
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2572
AB  - Objectives: Establishment of association between: (a) Val158Met COMT (G1947A) polymorphism and preeclampsia; (b) cytokines gene expression and COMT genotypes. Methods: 50 preeclampsia and 50 healthy pregnant women were enrolled. COMT genotyping was done by PCR/RFLP. TNF-α, IL-1β, and IL-6 mRNA levels were determined by Real-time PCR. Results: Variant (AA) homozygotes carried 3.7-fold increased preeclampsia odds, especially for severe (OR = 9.0, 95%CI (2.09-38.799)) and early forms (OR = 6.6, 95%CI (1.62-26.87)). AA homozygotes with PE had higher TNF-α levels compared to controls (P = 0.012). Conclusions: Val158Met COMT polymorphism increases preeclampsia risk. TNF-α expression and Val158Met COMT polymorphism have concomitant roles in PE pathogenesis.
PB  - Taylor & Francis Inc.
T2  - Hypertension in Pregnancy
T1  - VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia
VL  - 39
IS  - 4
SP  - 471
EP  - 480
DO  - 10.1080/10641955.2020.1843663
ER  - 

@article{
author = "Šljivančanin Jakovljević, Tamara and Kontić-Vučinić, Olivera and Nikolić, Nadja and Čarkić, Jelena and Milašin, Jelena",
year = "2020",
abstract = "Objectives: Establishment of association between: (a) Val158Met COMT (G1947A) polymorphism and preeclampsia; (b) cytokines gene expression and COMT genotypes. Methods: 50 preeclampsia and 50 healthy pregnant women were enrolled. COMT genotyping was done by PCR/RFLP. TNF-α, IL-1β, and IL-6 mRNA levels were determined by Real-time PCR. Results: Variant (AA) homozygotes carried 3.7-fold increased preeclampsia odds, especially for severe (OR = 9.0, 95%CI (2.09-38.799)) and early forms (OR = 6.6, 95%CI (1.62-26.87)). AA homozygotes with PE had higher TNF-α levels compared to controls (P = 0.012). Conclusions: Val158Met COMT polymorphism increases preeclampsia risk. TNF-α expression and Val158Met COMT polymorphism have concomitant roles in PE pathogenesis.",
publisher = "Taylor & Francis Inc.",
journal = "Hypertension in Pregnancy",
title = "VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia",
volume = "39",
number = "4",
pages = "471-480",
doi = "10.1080/10641955.2020.1843663"
}

Šljivančanin Jakovljević, T., Kontić-Vučinić, O., Nikolić, N., Čarkić, J.,& Milašin, J.. (2020). VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia. in Hypertension in Pregnancy
Taylor & Francis Inc.., 39(4), 471-480.
https://doi.org/10.1080/10641955.2020.1843663

Šljivančanin Jakovljević T, Kontić-Vučinić O, Nikolić N, Čarkić J, Milašin J. VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia. in Hypertension in Pregnancy. 2020;39(4):471-480.
doi:10.1080/10641955.2020.1843663 .

Šljivančanin Jakovljević, Tamara, Kontić-Vučinić, Olivera, Nikolić, Nadja, Čarkić, Jelena, Milašin, Jelena, "VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia" in Hypertension in Pregnancy, 39, no. 4 (2020):471-480,
https://doi.org/10.1080/10641955.2020.1843663 . .

TY  - JOUR
AU  - Matić Petrović, Sanja
AU  - Nikolić, Nadja
AU  - Toljić, Boško
AU  - Arambašić-Jovanović, Jelena
AU  - Miličić, Biljana
AU  - Miličić, Tanja
AU  - Jotić, Aleksandra
AU  - Vidaković, Melita
AU  - Milašin, Jelena
AU  - Pucar, Ana
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2571
AB  - Objectives: Aiming to show that periodontitis (PD) and type 2 diabetes (T2D) are bidirectionally related and potentially linked by inflammatory cytokines, we searched for association between -308 G/A Tumor necrosis factor-alpha (TNFα), +252A/G Lymphotoxin-alpha (LTα), +36A/G Tumor necrosis factor receptor 1 (TNFR1) and +676 T/G tumor necrosis factor receptor 2 (TNFR2) single nucleotide polymorphisms (SNPs) and: risk of PD or PD + T2D; periodontitis parameters in PD and PD + T2D; serum levels of cytokines/their receptors. Relationship between periodontal inflammation and serum cytokine/receptor levels was also assessed.

Design: Subjects were stratified as: 57 healthy controls (HC); 58 PD; 65 PD + T2D. Sociodemographic, environmental, behavioral and periodontal clinical data were recorded. SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism, while cytokines/receptors levels were quantified by enzyme-linked immunosorbent assay. Impact of periodontal inflammation was measured using periodontal inflamed surface area (PISA).

Results: TNFα AA genotype showed protective effect in T2D + PD compared to PD, even adjusted for behavioral/environmental factors (OR 0.18; 95 %CI 0.037-0.886; p = 0.035). LTα AG heterozygotes had increased risk of PD (OR 3.27; 95 %CI 1.35-7.96; p = 0.016), while TNFR2 TG genotype had protective effect (OR = 0.44; 95 %CI 0.954-0.9794; p = 0.043). TNFR1 AA was predictor of periodontal pocket depth and clinical attachment loss in PD. Correlation between TNFR2 concentration and PISA was negative in PD, positive in PD + T2D.

Conclusions: None of the SNPs showed cross-susceptibility between PD and T2D. + 252A/G LTα and +676 T/G TNFR2 SNPs are associated with PD risk. Periodontal destruction in healthy individuals is influenced by TNFR1 genotype. Impact of periodontal on systemic inflammation is masked by T2D.
PB  - Elsevier Inc.
T2  - Archives of Oral Biology
T1  - The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population
VL  - 120
SP  - 104929
DO  - 10.1016/j.archoralbio.2020.104929
ER  - 

@article{
author = "Matić Petrović, Sanja and Nikolić, Nadja and Toljić, Boško and Arambašić-Jovanović, Jelena and Miličić, Biljana and Miličić, Tanja and Jotić, Aleksandra and Vidaković, Melita and Milašin, Jelena and Pucar, Ana",
year = "2020",
abstract = "Objectives: Aiming to show that periodontitis (PD) and type 2 diabetes (T2D) are bidirectionally related and potentially linked by inflammatory cytokines, we searched for association between -308 G/A Tumor necrosis factor-alpha (TNFα), +252A/G Lymphotoxin-alpha (LTα), +36A/G Tumor necrosis factor receptor 1 (TNFR1) and +676 T/G tumor necrosis factor receptor 2 (TNFR2) single nucleotide polymorphisms (SNPs) and: risk of PD or PD + T2D; periodontitis parameters in PD and PD + T2D; serum levels of cytokines/their receptors. Relationship between periodontal inflammation and serum cytokine/receptor levels was also assessed.

Design: Subjects were stratified as: 57 healthy controls (HC); 58 PD; 65 PD + T2D. Sociodemographic, environmental, behavioral and periodontal clinical data were recorded. SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism, while cytokines/receptors levels were quantified by enzyme-linked immunosorbent assay. Impact of periodontal inflammation was measured using periodontal inflamed surface area (PISA).

Results: TNFα AA genotype showed protective effect in T2D + PD compared to PD, even adjusted for behavioral/environmental factors (OR 0.18; 95 %CI 0.037-0.886; p = 0.035). LTα AG heterozygotes had increased risk of PD (OR 3.27; 95 %CI 1.35-7.96; p = 0.016), while TNFR2 TG genotype had protective effect (OR = 0.44; 95 %CI 0.954-0.9794; p = 0.043). TNFR1 AA was predictor of periodontal pocket depth and clinical attachment loss in PD. Correlation between TNFR2 concentration and PISA was negative in PD, positive in PD + T2D.

Conclusions: None of the SNPs showed cross-susceptibility between PD and T2D. + 252A/G LTα and +676 T/G TNFR2 SNPs are associated with PD risk. Periodontal destruction in healthy individuals is influenced by TNFR1 genotype. Impact of periodontal on systemic inflammation is masked by T2D.",
publisher = "Elsevier Inc.",
journal = "Archives of Oral Biology",
title = "The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population",
volume = "120",
pages = "104929",
doi = "10.1016/j.archoralbio.2020.104929"
}

Matić Petrović, S., Nikolić, N., Toljić, B., Arambašić-Jovanović, J., Miličić, B., Miličić, T., Jotić, A., Vidaković, M., Milašin, J.,& Pucar, A.. (2020). The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population. in Archives of Oral Biology
Elsevier Inc.., 120, 104929.
https://doi.org/10.1016/j.archoralbio.2020.104929

Matić Petrović S, Nikolić N, Toljić B, Arambašić-Jovanović J, Miličić B, Miličić T, Jotić A, Vidaković M, Milašin J, Pucar A. The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population. in Archives of Oral Biology. 2020;120:104929.
doi:10.1016/j.archoralbio.2020.104929 .

Matić Petrović, Sanja, Nikolić, Nadja, Toljić, Boško, Arambašić-Jovanović, Jelena, Miličić, Biljana, Miličić, Tanja, Jotić, Aleksandra, Vidaković, Melita, Milašin, Jelena, Pucar, Ana, "The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population" in Archives of Oral Biology, 120 (2020):104929,
https://doi.org/10.1016/j.archoralbio.2020.104929 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Nikolić, Nadja
AU  - Jaćimović, Jelena
AU  - Pavlović, Ognjan
AU  - Miličić, Biljana
AU  - Beljić-Ivanović, Katarina
AU  - Miletić, Maja
AU  - Andrić, Miroslav
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2570
AB  - Introduction
 This study aimed to summarize data on apical periodontitis (AP) and nonsurgical root canal treatment (NSRCT) prevalence and risk factors related to age, gender, and quality of restorative and endodontic treatment in the general population from cross-sectional studies published between 2012 and 2020.
 
 Methods
 An electronic search was performed in the following databases: Web of Science, Scopus, and PubMed. The conducted literature search covered studies published between 2012 and 2020, without restrictions on language. The STROBE and NOS tools were used for quality assessment of the included studies.
 
 Results
 Sixteen articles were included in the review. In total, 200,041 teeth were examined. On average, 6.3% of teeth had AP, and 7.4% had NSRCT. Forty-one percent of RCT teeth had AP, and 3.5% of untreated teeth had AP. Female patients were less prone to AP in endodontically treated teeth only, compared with male patients (P < .001). Variable stratification of age subgroups among included studies prevented us from conducting a meta-analysis. An increase in AP frequency was found in teeth with inadequate restorative and endodontic treatment (P < .001 and P < .001, respectively). Because of high heterogeneity, these results should be taken with caution.
 
 Conclusions
 There is an increased AP prevalence in the adult general population compared with data from 2012 (6.3% versus 5.4%) in both endodontically treated (41.3% versus 35.9%) and untreated teeth (3.5% versus 2.1%). In addition, AP developed less frequently in female patients with endodontically treated teeth and in teeth with inadequate compared with adequate restorative and endodontic treatment.
PB  - Elsevier Inc.
T2  - Journal of Endodontics
T1  - Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020
VL  - 46
IS  - 10
SP  - 1371
EP  - 1386.e8
DO  - 10.1016/j.joen.2020.07.007
ER  - 

@article{
author = "Jakovljević, Aleksandar and Nikolić, Nadja and Jaćimović, Jelena and Pavlović, Ognjan and Miličić, Biljana and Beljić-Ivanović, Katarina and Miletić, Maja and Andrić, Miroslav and Milašin, Jelena",
year = "2020",
abstract = "Introduction
 This study aimed to summarize data on apical periodontitis (AP) and nonsurgical root canal treatment (NSRCT) prevalence and risk factors related to age, gender, and quality of restorative and endodontic treatment in the general population from cross-sectional studies published between 2012 and 2020.
 
 Methods
 An electronic search was performed in the following databases: Web of Science, Scopus, and PubMed. The conducted literature search covered studies published between 2012 and 2020, without restrictions on language. The STROBE and NOS tools were used for quality assessment of the included studies.
 
 Results
 Sixteen articles were included in the review. In total, 200,041 teeth were examined. On average, 6.3% of teeth had AP, and 7.4% had NSRCT. Forty-one percent of RCT teeth had AP, and 3.5% of untreated teeth had AP. Female patients were less prone to AP in endodontically treated teeth only, compared with male patients (P < .001). Variable stratification of age subgroups among included studies prevented us from conducting a meta-analysis. An increase in AP frequency was found in teeth with inadequate restorative and endodontic treatment (P < .001 and P < .001, respectively). Because of high heterogeneity, these results should be taken with caution.
 
 Conclusions
 There is an increased AP prevalence in the adult general population compared with data from 2012 (6.3% versus 5.4%) in both endodontically treated (41.3% versus 35.9%) and untreated teeth (3.5% versus 2.1%). In addition, AP developed less frequently in female patients with endodontically treated teeth and in teeth with inadequate compared with adequate restorative and endodontic treatment.",
publisher = "Elsevier Inc.",
journal = "Journal of Endodontics",
title = "Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020",
volume = "46",
number = "10",
pages = "1371-1386.e8",
doi = "10.1016/j.joen.2020.07.007"
}

Jakovljević, A., Nikolić, N., Jaćimović, J., Pavlović, O., Miličić, B., Beljić-Ivanović, K., Miletić, M., Andrić, M.,& Milašin, J.. (2020). Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020. in Journal of Endodontics
Elsevier Inc.., 46(10), 1371-1386.e8.
https://doi.org/10.1016/j.joen.2020.07.007

Jakovljević A, Nikolić N, Jaćimović J, Pavlović O, Miličić B, Beljić-Ivanović K, Miletić M, Andrić M, Milašin J. Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020. in Journal of Endodontics. 2020;46(10):1371-1386.e8.
doi:10.1016/j.joen.2020.07.007 .

Jakovljević, Aleksandar, Nikolić, Nadja, Jaćimović, Jelena, Pavlović, Ognjan, Miličić, Biljana, Beljić-Ivanović, Katarina, Miletić, Maja, Andrić, Miroslav, Milašin, Jelena, "Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020" in Journal of Endodontics, 46, no. 10 (2020):1371-1386.e8,
https://doi.org/10.1016/j.joen.2020.07.007 . .

TY  - JOUR
AU  - Šljivančanin Jakovljević, Tamara
AU  - Jaćimović, Jelena
AU  - Nikolić, Nadja
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2569
AB  - Insufficient response to oxidative stress in placenta is proposed as a contributing factor for preeclampsia (PE) development. Glutathione S-transferases (GST) have significant role in detoxification processes. Conflicting results were published by several research groups regarding GST T1 and GST M1 deletion polymorphism as risk factors for PE. The aim of the present meta-analysis was to get a better understanding of the impact of these polymorphisms in preeclampsia development. To identify relevant case-control studies, the author team searched Clarivate Analytics Web of Science, Scopus, PubMed, Cochrane Central Register of Controlled Trials, China National Knowledge Infrastructure, major subject journals, and gray literature. Pooled odds ratios and 95% confidence intervals for GST M1 and GST T1 deletion polymorphism and preeclampsia were derived from random effects models. This meta-analysis included 10 eligible studies. The pooled analyses showed no association between GST M1/GST T1 deletion polymorphisms and susceptibility to PE. Even though high heterogeneity was founded among results for GST M1 and double null genotypes, Egger's and Begg's tests (0.17 and 0.18, respectively) revealed no statistical evidence of publication bias among included studies. The present updated systematic review and meta-analysis found no association between GST M1 and GST T1 deletion polymorphism and PE risk.
PB  - Wiley
T2  - American Journal of Reproductive Immunology
T1  - Lack of association between glutathione S-transferase M1 and T1 gene polymorphisms and susceptibility to preeclampsia: An updated systematic review and meta-analysis
VL  - 84
IS  - 6
SP  - e13303
DO  - 10.1111/aji.13303
ER  - 

@article{
author = "Šljivančanin Jakovljević, Tamara and Jaćimović, Jelena and Nikolić, Nadja and Milašin, Jelena",
year = "2020",
abstract = "Insufficient response to oxidative stress in placenta is proposed as a contributing factor for preeclampsia (PE) development. Glutathione S-transferases (GST) have significant role in detoxification processes. Conflicting results were published by several research groups regarding GST T1 and GST M1 deletion polymorphism as risk factors for PE. The aim of the present meta-analysis was to get a better understanding of the impact of these polymorphisms in preeclampsia development. To identify relevant case-control studies, the author team searched Clarivate Analytics Web of Science, Scopus, PubMed, Cochrane Central Register of Controlled Trials, China National Knowledge Infrastructure, major subject journals, and gray literature. Pooled odds ratios and 95% confidence intervals for GST M1 and GST T1 deletion polymorphism and preeclampsia were derived from random effects models. This meta-analysis included 10 eligible studies. The pooled analyses showed no association between GST M1/GST T1 deletion polymorphisms and susceptibility to PE. Even though high heterogeneity was founded among results for GST M1 and double null genotypes, Egger's and Begg's tests (0.17 and 0.18, respectively) revealed no statistical evidence of publication bias among included studies. The present updated systematic review and meta-analysis found no association between GST M1 and GST T1 deletion polymorphism and PE risk.",
publisher = "Wiley",
journal = "American Journal of Reproductive Immunology",
title = "Lack of association between glutathione S-transferase M1 and T1 gene polymorphisms and susceptibility to preeclampsia: An updated systematic review and meta-analysis",
volume = "84",
number = "6",
pages = "e13303",
doi = "10.1111/aji.13303"
}

Šljivančanin Jakovljević, T., Jaćimović, J., Nikolić, N.,& Milašin, J.. (2020). Lack of association between glutathione S-transferase M1 and T1 gene polymorphisms and susceptibility to preeclampsia: An updated systematic review and meta-analysis. in American Journal of Reproductive Immunology
Wiley., 84(6), e13303.
https://doi.org/10.1111/aji.13303

Šljivančanin Jakovljević T, Jaćimović J, Nikolić N, Milašin J. Lack of association between glutathione S-transferase M1 and T1 gene polymorphisms and susceptibility to preeclampsia: An updated systematic review and meta-analysis. in American Journal of Reproductive Immunology. 2020;84(6):e13303.
doi:10.1111/aji.13303 .

Šljivančanin Jakovljević, Tamara, Jaćimović, Jelena, Nikolić, Nadja, Milašin, Jelena, "Lack of association between glutathione S-transferase M1 and T1 gene polymorphisms and susceptibility to preeclampsia: An updated systematic review and meta-analysis" in American Journal of Reproductive Immunology, 84, no. 6 (2020):e13303,
https://doi.org/10.1111/aji.13303 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Nikolić, Nadja
AU  - Jaćimović, Jelena
AU  - Pavlović, Ognjan
AU  - Miličić, Biljana
AU  - Beljić-Ivanović, Katarina
AU  - Miletić, Maja
AU  - Andrić, Miroslav
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2531
AB  - Introduction
This study aimed to summarize data on apical periodontitis (AP) and nonsurgical root canal treatment (NSRCT) prevalence and risk factors related to age, gender, and quality of restorative and endodontic treatment in the general population from cross-sectional studies published between 2012 and 2020.

Methods
An electronic search was performed in the following databases: Web of Science, Scopus, and PubMed. The conducted literature search covered studies published between 2012 and 2020, without restrictions on language. The STROBE and NOS tools were used for quality assessment of the included studies.

Results
Sixteen articles were included in the review. In total, 200,041 teeth were examined. On average, 6.3% of teeth had AP, and 7.4% had NSRCT. Forty-one percent of RCT teeth had AP, and 3.5% of untreated teeth had AP. Female patients were less prone to AP in endodontically treated teeth only, compared with male patients (P < .001). Variable stratification of age subgroups among included studies prevented us from conducting a meta-analysis. An increase in AP frequency was found in teeth with inadequate restorative and endodontic treatment (P < .001 and P < .001, respectively). Because of high heterogeneity, these results should be taken with caution.

Conclusions
There is an increased AP prevalence in the adult general population compared with data from 2012 (6.3% versus 5.4%) in both endodontically treated (41.3% versus 35.9%) and untreated teeth (3.5% versus 2.1%). In addition, AP developed less frequently in female patients with endodontically treated teeth and in teeth with inadequate compared with adequate restorative and endodontic treatment.
PB  - Elsevier Inc.
T2  - Journal of Endodontics
T1  - Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020
VL  - 46
IS  - 10
SP  - 1371
EP  - 1386
DO  - 10.1016/j.joen.2020.07.007
ER  - 

@article{
author = "Jakovljević, Aleksandar and Nikolić, Nadja and Jaćimović, Jelena and Pavlović, Ognjan and Miličić, Biljana and Beljić-Ivanović, Katarina and Miletić, Maja and Andrić, Miroslav and Milašin, Jelena",
year = "2020",
abstract = "Introduction
This study aimed to summarize data on apical periodontitis (AP) and nonsurgical root canal treatment (NSRCT) prevalence and risk factors related to age, gender, and quality of restorative and endodontic treatment in the general population from cross-sectional studies published between 2012 and 2020.

Methods
An electronic search was performed in the following databases: Web of Science, Scopus, and PubMed. The conducted literature search covered studies published between 2012 and 2020, without restrictions on language. The STROBE and NOS tools were used for quality assessment of the included studies.

Results
Sixteen articles were included in the review. In total, 200,041 teeth were examined. On average, 6.3% of teeth had AP, and 7.4% had NSRCT. Forty-one percent of RCT teeth had AP, and 3.5% of untreated teeth had AP. Female patients were less prone to AP in endodontically treated teeth only, compared with male patients (P < .001). Variable stratification of age subgroups among included studies prevented us from conducting a meta-analysis. An increase in AP frequency was found in teeth with inadequate restorative and endodontic treatment (P < .001 and P < .001, respectively). Because of high heterogeneity, these results should be taken with caution.

Conclusions
There is an increased AP prevalence in the adult general population compared with data from 2012 (6.3% versus 5.4%) in both endodontically treated (41.3% versus 35.9%) and untreated teeth (3.5% versus 2.1%). In addition, AP developed less frequently in female patients with endodontically treated teeth and in teeth with inadequate compared with adequate restorative and endodontic treatment.",
publisher = "Elsevier Inc.",
journal = "Journal of Endodontics",
title = "Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020",
volume = "46",
number = "10",
pages = "1371-1386",
doi = "10.1016/j.joen.2020.07.007"
}

Jakovljević, A., Nikolić, N., Jaćimović, J., Pavlović, O., Miličić, B., Beljić-Ivanović, K., Miletić, M., Andrić, M.,& Milašin, J.. (2020). Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020. in Journal of Endodontics
Elsevier Inc.., 46(10), 1371-1386.
https://doi.org/10.1016/j.joen.2020.07.007

Jakovljević A, Nikolić N, Jaćimović J, Pavlović O, Miličić B, Beljić-Ivanović K, Miletić M, Andrić M, Milašin J. Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020. in Journal of Endodontics. 2020;46(10):1371-1386.
doi:10.1016/j.joen.2020.07.007 .

Jakovljević, Aleksandar, Nikolić, Nadja, Jaćimović, Jelena, Pavlović, Ognjan, Miličić, Biljana, Beljić-Ivanović, Katarina, Miletić, Maja, Andrić, Miroslav, Milašin, Jelena, "Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020" in Journal of Endodontics, 46, no. 10 (2020):1371-1386,
https://doi.org/10.1016/j.joen.2020.07.007 . .

TY  - JOUR
AU  - Vučković, Mila
AU  - Nikolić, Nadja
AU  - Milašin, Jelena
AU  - Đorđević, Vladan
AU  - Milinković, Iva
AU  - Asotić, Jasminka
AU  - Jezdić, Zoran
AU  - Janković, Saša
AU  - Aleksić, Zoran
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2525
AB  - Introduction/Objective. The objective of the study was to investigate whether there are differences in therapeutic effect between initial treatments of chronic periodontitis [scaling and root planning (SRP)] alone and SRP in conjunction with injectable platelet-rich fibrin (I-PRF) application, comparing clinical parameters after three months. Methods. Twenty-four patients with chronic periodontitis who had at least two sites with probing pocket depth (PPD) ≥ 5 mm on contralateral side participated in the study. Using a split-mouth design, the patients were treated with SRP + I-PRF (study group) or SRP only (control group). The clinical parameters, clinical attachment level (CAL), gingival margin level (GML), PPD, bleeding on probing, and plaque index, were recorded on both sides. Results. Compared to baseline, both treatment modalities demonstrated an improvement in investigated clinical parameters. The mean value of CAL was reduced from 1.97 ± 0.75 (0.25–3.31) to 1.07 ± 0.44 (0.12–1.78) in the study group, whereas it decreased from 1.81 ± 0.66 (0.42–2.96) to 1.48 ± 0.55 (0.22–2.30) in the control group. Similarly, the corresponding values for GML and PPD showed statistically significant difference between the groups (p = 0.040 and p = 0.006, respectively). Conclusion. Regardless the limited number of patients in the study, initial periodontal therapy in conjunction with injectable platelet-rich fibrin proved to display significant improvement in all clinical parameters compared to initial periodontal therapy alone.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - The effect of injectable platelet-rich fibrin use in the initial treatment of chronic periodontitis
VL  - 148
IS  - 5-6
SP  - 280
EP  - 285
DO  - 10.2298/SARH190925022V
ER  - 

@article{
author = "Vučković, Mila and Nikolić, Nadja and Milašin, Jelena and Đorđević, Vladan and Milinković, Iva and Asotić, Jasminka and Jezdić, Zoran and Janković, Saša and Aleksić, Zoran",
year = "2020",
abstract = "Introduction/Objective. The objective of the study was to investigate whether there are differences in therapeutic effect between initial treatments of chronic periodontitis [scaling and root planning (SRP)] alone and SRP in conjunction with injectable platelet-rich fibrin (I-PRF) application, comparing clinical parameters after three months. Methods. Twenty-four patients with chronic periodontitis who had at least two sites with probing pocket depth (PPD) ≥ 5 mm on contralateral side participated in the study. Using a split-mouth design, the patients were treated with SRP + I-PRF (study group) or SRP only (control group). The clinical parameters, clinical attachment level (CAL), gingival margin level (GML), PPD, bleeding on probing, and plaque index, were recorded on both sides. Results. Compared to baseline, both treatment modalities demonstrated an improvement in investigated clinical parameters. The mean value of CAL was reduced from 1.97 ± 0.75 (0.25–3.31) to 1.07 ± 0.44 (0.12–1.78) in the study group, whereas it decreased from 1.81 ± 0.66 (0.42–2.96) to 1.48 ± 0.55 (0.22–2.30) in the control group. Similarly, the corresponding values for GML and PPD showed statistically significant difference between the groups (p = 0.040 and p = 0.006, respectively). Conclusion. Regardless the limited number of patients in the study, initial periodontal therapy in conjunction with injectable platelet-rich fibrin proved to display significant improvement in all clinical parameters compared to initial periodontal therapy alone.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "The effect of injectable platelet-rich fibrin use in the initial treatment of chronic periodontitis",
volume = "148",
number = "5-6",
pages = "280-285",
doi = "10.2298/SARH190925022V"
}

Vučković, M., Nikolić, N., Milašin, J., Đorđević, V., Milinković, I., Asotić, J., Jezdić, Z., Janković, S.,& Aleksić, Z.. (2020). The effect of injectable platelet-rich fibrin use in the initial treatment of chronic periodontitis. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 148(5-6), 280-285.
https://doi.org/10.2298/SARH190925022V

Vučković M, Nikolić N, Milašin J, Đorđević V, Milinković I, Asotić J, Jezdić Z, Janković S, Aleksić Z. The effect of injectable platelet-rich fibrin use in the initial treatment of chronic periodontitis. in Srpski arhiv za celokupno lekarstvo. 2020;148(5-6):280-285.
doi:10.2298/SARH190925022V .

Vučković, Mila, Nikolić, Nadja, Milašin, Jelena, Đorđević, Vladan, Milinković, Iva, Asotić, Jasminka, Jezdić, Zoran, Janković, Saša, Aleksić, Zoran, "The effect of injectable platelet-rich fibrin use in the initial treatment of chronic periodontitis" in Srpski arhiv za celokupno lekarstvo, 148, no. 5-6 (2020):280-285,
https://doi.org/10.2298/SARH190925022V . .

TY  - JOUR
AU  - Popovac, Aleksandra
AU  - Mladenović, Irena
AU  - Krunić, Jelena
AU  - Trifković, Branka
AU  - Todorović, Ana
AU  - Milašin, Jelena
AU  - Despotović, Nebojša
AU  - Stančić, Ivica
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2517
AB  - Compromised dentition has been suggested to pose a significant risk factor for dementia. It was mainly investigated through insufficient tooth number, disregarding contact between opposing teeth (dental occlusion). The epsilon 4 allele of apolipoprotein (APOE4) is the primary genetic marker for the late onset of Alzheimer's disease (AD). However, APOE4 and dental occlusion have not yet been investigated as possible associated risk factors for AD. The study was aimed to examine the impact of dental status and different APOE gene variants on AD occurrence. Secondly, sociodemographic variables were investigated as factors potentially associated with AD. The case-control study included two groups: 116 patients with AD (according to the NINDS-ADRDA criteria) and 63 controls (Mini-Mental State Examination scores >= 24). The analysis of APOE gene polymorphism was conducted through PCR reaction. Dental examination included recording of number of teeth, presence of fixed or removable dentures, and number of functional tooth units (FTU). Regression analysis was used to investigate the joint effect of the clinical and genetic variables on AD. Results showed that patients with AD were more often carriers of epsilon 3/epsilon 4 genotype and epsilon 4 allele, had lower number of teeth and FTU, and were less likely to be married, live in home, and had less chronic diseases, compared to the controls. Regression analysis showed that presence of APOE4 allele and the number of total FTU remained associated with AD, even when adjusted for age, sex, and level of education. In conclusion, deficient dental occlusion and presence of APOE4 may independently increase risk for AD.
PB  - Ios Press, Amsterdam
T2  - Journal of Alzheimers Disease
T1  - Apolipoprotein epsilon 4 Allele and Dental Occlusion Deficiency as Risk Factors for Alzheimer's Disease
VL  - 74
IS  - 3
SP  - 797
EP  - 802
DO  - 10.3233/JAD-191283
ER  - 

@article{
author = "Popovac, Aleksandra and Mladenović, Irena and Krunić, Jelena and Trifković, Branka and Todorović, Ana and Milašin, Jelena and Despotović, Nebojša and Stančić, Ivica",
year = "2020",
abstract = "Compromised dentition has been suggested to pose a significant risk factor for dementia. It was mainly investigated through insufficient tooth number, disregarding contact between opposing teeth (dental occlusion). The epsilon 4 allele of apolipoprotein (APOE4) is the primary genetic marker for the late onset of Alzheimer's disease (AD). However, APOE4 and dental occlusion have not yet been investigated as possible associated risk factors for AD. The study was aimed to examine the impact of dental status and different APOE gene variants on AD occurrence. Secondly, sociodemographic variables were investigated as factors potentially associated with AD. The case-control study included two groups: 116 patients with AD (according to the NINDS-ADRDA criteria) and 63 controls (Mini-Mental State Examination scores >= 24). The analysis of APOE gene polymorphism was conducted through PCR reaction. Dental examination included recording of number of teeth, presence of fixed or removable dentures, and number of functional tooth units (FTU). Regression analysis was used to investigate the joint effect of the clinical and genetic variables on AD. Results showed that patients with AD were more often carriers of epsilon 3/epsilon 4 genotype and epsilon 4 allele, had lower number of teeth and FTU, and were less likely to be married, live in home, and had less chronic diseases, compared to the controls. Regression analysis showed that presence of APOE4 allele and the number of total FTU remained associated with AD, even when adjusted for age, sex, and level of education. In conclusion, deficient dental occlusion and presence of APOE4 may independently increase risk for AD.",
publisher = "Ios Press, Amsterdam",
journal = "Journal of Alzheimers Disease",
title = "Apolipoprotein epsilon 4 Allele and Dental Occlusion Deficiency as Risk Factors for Alzheimer's Disease",
volume = "74",
number = "3",
pages = "797-802",
doi = "10.3233/JAD-191283"
}

Popovac, A., Mladenović, I., Krunić, J., Trifković, B., Todorović, A., Milašin, J., Despotović, N.,& Stančić, I.. (2020). Apolipoprotein epsilon 4 Allele and Dental Occlusion Deficiency as Risk Factors for Alzheimer's Disease. in Journal of Alzheimers Disease
Ios Press, Amsterdam., 74(3), 797-802.
https://doi.org/10.3233/JAD-191283

Popovac A, Mladenović I, Krunić J, Trifković B, Todorović A, Milašin J, Despotović N, Stančić I. Apolipoprotein epsilon 4 Allele and Dental Occlusion Deficiency as Risk Factors for Alzheimer's Disease. in Journal of Alzheimers Disease. 2020;74(3):797-802.
doi:10.3233/JAD-191283 .

Popovac, Aleksandra, Mladenović, Irena, Krunić, Jelena, Trifković, Branka, Todorović, Ana, Milašin, Jelena, Despotović, Nebojša, Stančić, Ivica, "Apolipoprotein epsilon 4 Allele and Dental Occlusion Deficiency as Risk Factors for Alzheimer's Disease" in Journal of Alzheimers Disease, 74, no. 3 (2020):797-802,
https://doi.org/10.3233/JAD-191283 . .

TY  - JOUR
AU  - Oprić, D.
AU  - Stankovich, A.D.
AU  - Nenadović, A.
AU  - Kovačević, S.
AU  - Obradović, D.D.
AU  - de Luka, Silvio R.
AU  - Nešović-Ostojić, J.
AU  - Milašin, Jelena
AU  - Ilić, A.Ž.
AU  - Trbovich, Alexander M.
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2506
AB  - Objective: Inflammation is a biological response of tissue to harmful stimuli. A high-fat diet was linked to low-grade chronic liver inflammation, which can further lead to more severe health conditions. It is crucial to assess the intensity of inflammation and structural tissue changes to reduce the subjective judgment by the examiner. We propose fractal-based methods for early-stage low-degree liver inflammation grading. Methods: We have randomly divided 40 C57BL/6 female mice into four groups (control, linseed oil, palm oil, sunflower oil). After 100 days, animals were euthanized, and liver tissue collected for analyses. We performed calculations of fractal dimension, fractal lacunarity, multifractal spectra, local fractal dimension, and particle metrics, applicable to tissue segmentation and grading. Results: Pathohistological analysis of some liver tissue showed a low-grade inflammatory infiltrate around the portal vein of experimental groups subjected to different high-fat diets. Differences in fractal dimension and lacunarity of the inflamed tissue were, in most cases, statistically significant between the high-fat diet groups. Both the observed intensity and area of inflammation were lowest for the sunflower oil. The results of standard fractal analysis, local fractal analysis, and particle analysis were in an excellent agreement. Conclusions: This study demonstrated the efficiency of the fractal analysis based tools in the quantification of complexity and early-stage structural changes in inflamed liver tissue, which could potentially be used in the diagnostic workup of inflammation in the liver. The presented methods could be implemented within a wider scope computer-aided diagnostics system in a very straightforward manner.
PB  - Elsevier Ltd
T2  - Biomedical Signal Processing & Control
T1  - Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils
VL  - 61
DO  - 10.1016/j.bspc.2020.101959
ER  - 

@article{
author = "Oprić, D. and Stankovich, A.D. and Nenadović, A. and Kovačević, S. and Obradović, D.D. and de Luka, Silvio R. and Nešović-Ostojić, J. and Milašin, Jelena and Ilić, A.Ž. and Trbovich, Alexander M.",
year = "2020",
abstract = "Objective: Inflammation is a biological response of tissue to harmful stimuli. A high-fat diet was linked to low-grade chronic liver inflammation, which can further lead to more severe health conditions. It is crucial to assess the intensity of inflammation and structural tissue changes to reduce the subjective judgment by the examiner. We propose fractal-based methods for early-stage low-degree liver inflammation grading. Methods: We have randomly divided 40 C57BL/6 female mice into four groups (control, linseed oil, palm oil, sunflower oil). After 100 days, animals were euthanized, and liver tissue collected for analyses. We performed calculations of fractal dimension, fractal lacunarity, multifractal spectra, local fractal dimension, and particle metrics, applicable to tissue segmentation and grading. Results: Pathohistological analysis of some liver tissue showed a low-grade inflammatory infiltrate around the portal vein of experimental groups subjected to different high-fat diets. Differences in fractal dimension and lacunarity of the inflamed tissue were, in most cases, statistically significant between the high-fat diet groups. Both the observed intensity and area of inflammation were lowest for the sunflower oil. The results of standard fractal analysis, local fractal analysis, and particle analysis were in an excellent agreement. Conclusions: This study demonstrated the efficiency of the fractal analysis based tools in the quantification of complexity and early-stage structural changes in inflamed liver tissue, which could potentially be used in the diagnostic workup of inflammation in the liver. The presented methods could be implemented within a wider scope computer-aided diagnostics system in a very straightforward manner.",
publisher = "Elsevier Ltd",
journal = "Biomedical Signal Processing & Control",
title = "Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils",
volume = "61",
doi = "10.1016/j.bspc.2020.101959"
}

Oprić, D., Stankovich, A.D., Nenadović, A., Kovačević, S., Obradović, D.D., de Luka, S. R., Nešović-Ostojić, J., Milašin, J., Ilić, A.Ž.,& Trbovich, A. M.. (2020). Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils. in Biomedical Signal Processing & Control
Elsevier Ltd., 61.
https://doi.org/10.1016/j.bspc.2020.101959

Oprić D, Stankovich A, Nenadović A, Kovačević S, Obradović D, de Luka SR, Nešović-Ostojić J, Milašin J, Ilić A, Trbovich AM. Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils. in Biomedical Signal Processing & Control. 2020;61.
doi:10.1016/j.bspc.2020.101959 .

Oprić, D., Stankovich, A.D., Nenadović, A., Kovačević, S., Obradović, D.D., de Luka, Silvio R., Nešović-Ostojić, J., Milašin, Jelena, Ilić, A.Ž., Trbovich, Alexander M., "Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils" in Biomedical Signal Processing & Control, 61 (2020),
https://doi.org/10.1016/j.bspc.2020.101959 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Nikolić, Nadja
AU  - Čarkić, Jelena
AU  - Andrić, Miroslav
AU  - Miletić, Maja
AU  - Beljić-Ivanović, Katarina
AU  - Jovanović, Tanja
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2493
AB  - Objectives: This study aimed to investigate whether Epstein-Barr virus (EBV) positive periapical lesions exhibited higher mRNA levels of Notch signalling molecules (Notch2 and Jagged1), bone resorption regulators (receptor activator of nuclear factor kappa-? ligand (RANKL) and osteoprotegerin (OPG)), and proinflammatory cytokines (tumour necrosis factor-alpha (TNF-?), interleukin-1 beta (IL-1?) and IL-6) compared to EBV negative lesions. Additionally, the potential correlation between investigated molecules in periapical lesions was analyzed. Materials and methods: Sixty-four apical periodontitis lesions were obtained subsequent to standard apicoectomy procedure. The presence of EBV was determined using nested PCR. Based on the presence of EBV all periapical lesions were divided into two groups, 29 EBV positive and 35 EBV negative lesions. A reverse transcriptase real-time PCR was used to determine mRNA levels of Notch2, Jagged1, RANKL, OPG, TNF-?, IL-1? and IL-6. Results: Significantly higher mRNA levels of Notch2, Jagged1, RANKL and IL-1? were observed in EBV positive compared to EBV negative lesions. Significant positive correlation was present between Notch2 and Jagged1, Jagged1 and RANKL, and IL-? and TNF-? in EBV positive periapical lesions. Conclusions: Notch signalling pathway may be involved in alveolar bone resorption in apical periodontitis lesions infected by EBV.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Acta Odontologica Scandinavica
T1  - Notch ? a possible mediator between Epstein-Barr virus infection and bone resorption in apical periodontitis
VL  - 78
IS  - 2
SP  - 126
EP  - 131
DO  - 10.1080/00016357.2019.1658896
ER  - 

@article{
author = "Jakovljević, Aleksandar and Nikolić, Nadja and Čarkić, Jelena and Andrić, Miroslav and Miletić, Maja and Beljić-Ivanović, Katarina and Jovanović, Tanja and Milašin, Jelena",
year = "2020",
abstract = "Objectives: This study aimed to investigate whether Epstein-Barr virus (EBV) positive periapical lesions exhibited higher mRNA levels of Notch signalling molecules (Notch2 and Jagged1), bone resorption regulators (receptor activator of nuclear factor kappa-? ligand (RANKL) and osteoprotegerin (OPG)), and proinflammatory cytokines (tumour necrosis factor-alpha (TNF-?), interleukin-1 beta (IL-1?) and IL-6) compared to EBV negative lesions. Additionally, the potential correlation between investigated molecules in periapical lesions was analyzed. Materials and methods: Sixty-four apical periodontitis lesions were obtained subsequent to standard apicoectomy procedure. The presence of EBV was determined using nested PCR. Based on the presence of EBV all periapical lesions were divided into two groups, 29 EBV positive and 35 EBV negative lesions. A reverse transcriptase real-time PCR was used to determine mRNA levels of Notch2, Jagged1, RANKL, OPG, TNF-?, IL-1? and IL-6. Results: Significantly higher mRNA levels of Notch2, Jagged1, RANKL and IL-1? were observed in EBV positive compared to EBV negative lesions. Significant positive correlation was present between Notch2 and Jagged1, Jagged1 and RANKL, and IL-? and TNF-? in EBV positive periapical lesions. Conclusions: Notch signalling pathway may be involved in alveolar bone resorption in apical periodontitis lesions infected by EBV.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Acta Odontologica Scandinavica",
title = "Notch ? a possible mediator between Epstein-Barr virus infection and bone resorption in apical periodontitis",
volume = "78",
number = "2",
pages = "126-131",
doi = "10.1080/00016357.2019.1658896"
}

Jakovljević, A., Nikolić, N., Čarkić, J., Andrić, M., Miletić, M., Beljić-Ivanović, K., Jovanović, T.,& Milašin, J.. (2020). Notch ? a possible mediator between Epstein-Barr virus infection and bone resorption in apical periodontitis. in Acta Odontologica Scandinavica
Taylor & Francis Ltd, Abingdon., 78(2), 126-131.
https://doi.org/10.1080/00016357.2019.1658896

Jakovljević A, Nikolić N, Čarkić J, Andrić M, Miletić M, Beljić-Ivanović K, Jovanović T, Milašin J. Notch ? a possible mediator between Epstein-Barr virus infection and bone resorption in apical periodontitis. in Acta Odontologica Scandinavica. 2020;78(2):126-131.
doi:10.1080/00016357.2019.1658896 .

Jakovljević, Aleksandar, Nikolić, Nadja, Čarkić, Jelena, Andrić, Miroslav, Miletić, Maja, Beljić-Ivanović, Katarina, Jovanović, Tanja, Milašin, Jelena, "Notch ? a possible mediator between Epstein-Barr virus infection and bone resorption in apical periodontitis" in Acta Odontologica Scandinavica, 78, no. 2 (2020):126-131,
https://doi.org/10.1080/00016357.2019.1658896 . .

TY  - JOUR
AU  - Mijailović, Iva
AU  - Nikolić, Nadja
AU  - Đinić, Ana
AU  - Čarkić, Jelena
AU  - Milinković, Iva
AU  - Perić, Mina
AU  - Janković, Saša
AU  - Milašin, Jelena
AU  - Aleksić, Zoran
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2492
AB  - Background The exact mechanisms of bone resorption in periodontitis have not been fully elucidated. The aims of this study were to analyze the expression of Notch signaling molecules, bone remodeling mediators, and pro-inflammatory cytokines in periodontitis patients and to determine their potential correlations. Methods The study included 130 individuals: 40 with aggressive periodontitis (AP group), 40 with chronic periodontitis (CP group), and 50 periodontally healthy controls. Total RNA was extracted from gingival crevicular fluid samples and relative gene expression of investigated molecules (Notch 1, Notch 2, Jagged 1, Hes 1, Hey 1, TNF-alpha, IL-17, RANKL, and OPG) was determined by reverse transcriptase - real-time polymerase chain reaction (RT-qPCR). Results In AP group, a significant increase of Notch 2, TNF-alpha, IL-17 and RANKL and a significant decrease of Notch 1 and Jagged 1 expression were observed compared to control group (P = 0.023, P = 0.005, P = 0.030, and P = 0.001 P = 0.031 and P = 0.029, respectively). Notch 2 and RANKL were also overexpressed in CP group compared to controls (P = 0.001 and P = 0.011). Significant correlations were observed in AP group between expression levels of the analyzed genes. Conclusion The present findings implicate Notch 2 overexpression in the ethiopathogenesis of bone resorption in aggressive and chronic periodontitis. The down-regulation of Notch 1 and Jagged 1 and loss of their osteoprotective function might cause a more excessive osteoclast formation and contribute to greater osteolysis in aggressive periodontitis.
PB  - Wiley, Hoboken
T2  - Journal of Periodontology
T1  - The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis
VL  - 91
IS  - 4
SP  - 554
EP  - 561
DO  - 10.1002/JPER.18-0755
ER  - 

@article{
author = "Mijailović, Iva and Nikolić, Nadja and Đinić, Ana and Čarkić, Jelena and Milinković, Iva and Perić, Mina and Janković, Saša and Milašin, Jelena and Aleksić, Zoran",
year = "2020",
abstract = "Background The exact mechanisms of bone resorption in periodontitis have not been fully elucidated. The aims of this study were to analyze the expression of Notch signaling molecules, bone remodeling mediators, and pro-inflammatory cytokines in periodontitis patients and to determine their potential correlations. Methods The study included 130 individuals: 40 with aggressive periodontitis (AP group), 40 with chronic periodontitis (CP group), and 50 periodontally healthy controls. Total RNA was extracted from gingival crevicular fluid samples and relative gene expression of investigated molecules (Notch 1, Notch 2, Jagged 1, Hes 1, Hey 1, TNF-alpha, IL-17, RANKL, and OPG) was determined by reverse transcriptase - real-time polymerase chain reaction (RT-qPCR). Results In AP group, a significant increase of Notch 2, TNF-alpha, IL-17 and RANKL and a significant decrease of Notch 1 and Jagged 1 expression were observed compared to control group (P = 0.023, P = 0.005, P = 0.030, and P = 0.001 P = 0.031 and P = 0.029, respectively). Notch 2 and RANKL were also overexpressed in CP group compared to controls (P = 0.001 and P = 0.011). Significant correlations were observed in AP group between expression levels of the analyzed genes. Conclusion The present findings implicate Notch 2 overexpression in the ethiopathogenesis of bone resorption in aggressive and chronic periodontitis. The down-regulation of Notch 1 and Jagged 1 and loss of their osteoprotective function might cause a more excessive osteoclast formation and contribute to greater osteolysis in aggressive periodontitis.",
publisher = "Wiley, Hoboken",
journal = "Journal of Periodontology",
title = "The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis",
volume = "91",
number = "4",
pages = "554-561",
doi = "10.1002/JPER.18-0755"
}

Mijailović, I., Nikolić, N., Đinić, A., Čarkić, J., Milinković, I., Perić, M., Janković, S., Milašin, J.,& Aleksić, Z.. (2020). The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis. in Journal of Periodontology
Wiley, Hoboken., 91(4), 554-561.
https://doi.org/10.1002/JPER.18-0755

Mijailović I, Nikolić N, Đinić A, Čarkić J, Milinković I, Perić M, Janković S, Milašin J, Aleksić Z. The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis. in Journal of Periodontology. 2020;91(4):554-561.
doi:10.1002/JPER.18-0755 .

Mijailović, Iva, Nikolić, Nadja, Đinić, Ana, Čarkić, Jelena, Milinković, Iva, Perić, Mina, Janković, Saša, Milašin, Jelena, Aleksić, Zoran, "The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis" in Journal of Periodontology, 91, no. 4 (2020):554-561,
https://doi.org/10.1002/JPER.18-0755 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2486
PB  - Association for Dental Sciences of the Republic of China
T2  - Journal of Dental Sciences
T1  - Herpesviral-bacterial synergy in the pathogenesis of apical periodontitis: New insights and future perspectives
DO  - 10.1016/j.jds.2020.05.001
ER  - 

@article{
author = "Jakovljević, Aleksandar and Milašin, Jelena",
year = "2020",
publisher = "Association for Dental Sciences of the Republic of China",
journal = "Journal of Dental Sciences",
title = "Herpesviral-bacterial synergy in the pathogenesis of apical periodontitis: New insights and future perspectives",
doi = "10.1016/j.jds.2020.05.001"
}

Jakovljević, A.,& Milašin, J.. (2020). Herpesviral-bacterial synergy in the pathogenesis of apical periodontitis: New insights and future perspectives. in Journal of Dental Sciences
Association for Dental Sciences of the Republic of China..
https://doi.org/10.1016/j.jds.2020.05.001

Jakovljević A, Milašin J. Herpesviral-bacterial synergy in the pathogenesis of apical periodontitis: New insights and future perspectives. in Journal of Dental Sciences. 2020;.
doi:10.1016/j.jds.2020.05.001 .

Jakovljević, Aleksandar, Milašin, Jelena, "Herpesviral-bacterial synergy in the pathogenesis of apical periodontitis: New insights and future perspectives" in Journal of Dental Sciences (2020),
https://doi.org/10.1016/j.jds.2020.05.001 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Nikolić, Nadja
AU  - Čarkić, Jelena
AU  - Beljić-Ivanović, Katarina
AU  - Soldatović, Ivan
AU  - Miletić, Maja
AU  - Andrić, Miroslav
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1047
AB  - Aim To investigate the possible association between TNF alpha (-308 G/A) and IL-1 beta (-511 C/T) single nucleotide polymorphisms (SNPs) and GSTT and GSTM deletion polymorphisms and risk of apical periodontitis (AP) development, and determine the association of different genotypes with the presence of herpesviral infection in AP. Methodology The study included 120 periapical lesions and 200 control samples. Gene polymorphism analysis was performed using either polymerase chain reaction (PCR) or PCR/ restriction fragment length polymorphism (RFLP). Relative gene expression of TNF-alpha and IL-1 beta was analysed using reverse transcriptase - real-time PCR. The presence of Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) was assessed by nested PCR. Chi-square and Fisher's exact tests and logistic regression analyses were done for polymorphisms, whilst Mann-Whitney U-test was performed for the expression analysis. The expected frequency of variants was analysed by the Hardy-Weinberg equilibrium test. Results TNF-alpha (-308 G/A) SNP increased AP susceptibility for heterozygous (odds ratio (OR) = 1.72, 95% confidence interval (CI) = 1.06-2.80, P = 0.027) and homozygous (OR = 8.55, 95% CI = 1.77-41.36, P  lt  0.001) carriers of the variant A allele. On the other hand, IL-1 beta (-511 C/T) polymorphism exerted a protective effect both in heterozygotes (OR = 0.540, 95% CI = 0.332-0.880, P = 0.013) and homozygotes (OR = 0.114, 95% CI = 0.026-0.501, P  lt  0.001). In addition, GSTM1 and GSTT1 null genotypes separately, as well as concomitantly, were associated with an increased risk for AP development (P  lt  0.001). The null GSTT1 genotype increased approximately twice the risk of Epstein-Barr infection (EBV) in AP (OR = 2.17, 95% CI = 1-4.71, P = 0.048), whilst TNF-alpha SNP decreased it, both in heterozygotes (OR = 0.20, 95% CI = 0.08-0.48, P  lt  0.001) and AA homozygotes (OR = 0.07, 95% CI = 0.01-0.37, P = 0.001). Conclusions GSTM and GSTT deletion polymorphisms, as well as TNF alpha (-308 G/A) SNP, are associated with increased risk, whereas IL-1 beta (-511 C/T) polymorphism decreases the risk of AP development. GSTT and TNF alpha polymorphisms also appear to modulate the risk of EBV infection in Serbian patients with AP.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Association of polymorphisms in TNF-alpha, IL-1 beta, GSTM and GSTT genes with apical periodontitis: is there a link with herpesviral infection?
VL  - 53
IS  - 7
SP  - 895
EP  - 904
DO  - 10.1111/iej.13298
ER  - 

@article{
author = "Jakovljević, Aleksandar and Nikolić, Nadja and Čarkić, Jelena and Beljić-Ivanović, Katarina and Soldatović, Ivan and Miletić, Maja and Andrić, Miroslav and Milašin, Jelena",
year = "2020",
abstract = "Aim To investigate the possible association between TNF alpha (-308 G/A) and IL-1 beta (-511 C/T) single nucleotide polymorphisms (SNPs) and GSTT and GSTM deletion polymorphisms and risk of apical periodontitis (AP) development, and determine the association of different genotypes with the presence of herpesviral infection in AP. Methodology The study included 120 periapical lesions and 200 control samples. Gene polymorphism analysis was performed using either polymerase chain reaction (PCR) or PCR/ restriction fragment length polymorphism (RFLP). Relative gene expression of TNF-alpha and IL-1 beta was analysed using reverse transcriptase - real-time PCR. The presence of Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) was assessed by nested PCR. Chi-square and Fisher's exact tests and logistic regression analyses were done for polymorphisms, whilst Mann-Whitney U-test was performed for the expression analysis. The expected frequency of variants was analysed by the Hardy-Weinberg equilibrium test. Results TNF-alpha (-308 G/A) SNP increased AP susceptibility for heterozygous (odds ratio (OR) = 1.72, 95% confidence interval (CI) = 1.06-2.80, P = 0.027) and homozygous (OR = 8.55, 95% CI = 1.77-41.36, P  lt  0.001) carriers of the variant A allele. On the other hand, IL-1 beta (-511 C/T) polymorphism exerted a protective effect both in heterozygotes (OR = 0.540, 95% CI = 0.332-0.880, P = 0.013) and homozygotes (OR = 0.114, 95% CI = 0.026-0.501, P  lt  0.001). In addition, GSTM1 and GSTT1 null genotypes separately, as well as concomitantly, were associated with an increased risk for AP development (P  lt  0.001). The null GSTT1 genotype increased approximately twice the risk of Epstein-Barr infection (EBV) in AP (OR = 2.17, 95% CI = 1-4.71, P = 0.048), whilst TNF-alpha SNP decreased it, both in heterozygotes (OR = 0.20, 95% CI = 0.08-0.48, P  lt  0.001) and AA homozygotes (OR = 0.07, 95% CI = 0.01-0.37, P = 0.001). Conclusions GSTM and GSTT deletion polymorphisms, as well as TNF alpha (-308 G/A) SNP, are associated with increased risk, whereas IL-1 beta (-511 C/T) polymorphism decreases the risk of AP development. GSTT and TNF alpha polymorphisms also appear to modulate the risk of EBV infection in Serbian patients with AP.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Association of polymorphisms in TNF-alpha, IL-1 beta, GSTM and GSTT genes with apical periodontitis: is there a link with herpesviral infection?",
volume = "53",
number = "7",
pages = "895-904",
doi = "10.1111/iej.13298"
}

Jakovljević, A., Nikolić, N., Čarkić, J., Beljić-Ivanović, K., Soldatović, I., Miletić, M., Andrić, M.,& Milašin, J.. (2020). Association of polymorphisms in TNF-alpha, IL-1 beta, GSTM and GSTT genes with apical periodontitis: is there a link with herpesviral infection?. in International Endodontic Journal
Wiley, Hoboken., 53(7), 895-904.
https://doi.org/10.1111/iej.13298

Jakovljević A, Nikolić N, Čarkić J, Beljić-Ivanović K, Soldatović I, Miletić M, Andrić M, Milašin J. Association of polymorphisms in TNF-alpha, IL-1 beta, GSTM and GSTT genes with apical periodontitis: is there a link with herpesviral infection?. in International Endodontic Journal. 2020;53(7):895-904.
doi:10.1111/iej.13298 .

Jakovljević, Aleksandar, Nikolić, Nadja, Čarkić, Jelena, Beljić-Ivanović, Katarina, Soldatović, Ivan, Miletić, Maja, Andrić, Miroslav, Milašin, Jelena, "Association of polymorphisms in TNF-alpha, IL-1 beta, GSTM and GSTT genes with apical periodontitis: is there a link with herpesviral infection?" in International Endodontic Journal, 53, no. 7 (2020):895-904,
https://doi.org/10.1111/iej.13298 . .

TY  - JOUR
AU  - Sabeti, Mohammad
AU  - Golchert, Kory J.
AU  - Shirgill, Neveed
AU  - Jakovljević, Aleksandar
AU  - Andrić, Miroslav
AU  - Milašin, Jelena
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2583
AB  - There is a need for updating the current treatment modalities for periapical lesions of endodontic origin to reflect the contributions and potential morbidity from herpesvirus infection. Herpesviruses, particularly human cytomegalovirus and Epstein-Barr virus, play an important role in the symptomatic periapical lesion. Indeed, periapical lesions may remain stable and silent until they become symptomatic, possibly triggered by the activation of latent herpesviruses within the lesion itself. Given the potential for grave systemic conditions associated with herpesvirus infection, early detection and treatment decisions for periapical lesions by the dentist may positively affect a patient’s overall health; especially for the immunocompromised patient.
PB  - Iris Publishers
T2  - Online Journal of Dentistry & Oral Health
T1  - Endodontic Periapical Disease: A Virus-Based Perspective
VL  - 2
IS  - 2
SP  - 1
EP  - 5
DO  - 10.33552/OJDOH.2019.02.000534
ER  - 

@article{
author = "Sabeti, Mohammad and Golchert, Kory J. and Shirgill, Neveed and Jakovljević, Aleksandar and Andrić, Miroslav and Milašin, Jelena",
year = "2019",
abstract = "There is a need for updating the current treatment modalities for periapical lesions of endodontic origin to reflect the contributions and potential morbidity from herpesvirus infection. Herpesviruses, particularly human cytomegalovirus and Epstein-Barr virus, play an important role in the symptomatic periapical lesion. Indeed, periapical lesions may remain stable and silent until they become symptomatic, possibly triggered by the activation of latent herpesviruses within the lesion itself. Given the potential for grave systemic conditions associated with herpesvirus infection, early detection and treatment decisions for periapical lesions by the dentist may positively affect a patient’s overall health; especially for the immunocompromised patient.",
publisher = "Iris Publishers",
journal = "Online Journal of Dentistry & Oral Health",
title = "Endodontic Periapical Disease: A Virus-Based Perspective",
volume = "2",
number = "2",
pages = "1-5",
doi = "10.33552/OJDOH.2019.02.000534"
}

Sabeti, M., Golchert, K. J., Shirgill, N., Jakovljević, A., Andrić, M.,& Milašin, J.. (2019). Endodontic Periapical Disease: A Virus-Based Perspective. in Online Journal of Dentistry & Oral Health
Iris Publishers., 2(2), 1-5.
https://doi.org/10.33552/OJDOH.2019.02.000534

Sabeti M, Golchert KJ, Shirgill N, Jakovljević A, Andrić M, Milašin J. Endodontic Periapical Disease: A Virus-Based Perspective. in Online Journal of Dentistry & Oral Health. 2019;2(2):1-5.
doi:10.33552/OJDOH.2019.02.000534 .

Sabeti, Mohammad, Golchert, Kory J., Shirgill, Neveed, Jakovljević, Aleksandar, Andrić, Miroslav, Milašin, Jelena, "Endodontic Periapical Disease: A Virus-Based Perspective" in Online Journal of Dentistry & Oral Health, 2, no. 2 (2019):1-5,
https://doi.org/10.33552/OJDOH.2019.02.000534 . .