TY  - JOUR
AU  - Petrović, Nina
AU  - Mandusić, Vesna
AU  - Dimitrijević, Bogomir
AU  - Roganović, Jelena
AU  - Lukić, Silvana
AU  - Todorović, Lidija
AU  - Stanojević, Boban
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1930
AB  - MicroRNAs play essential role in breast carcinoma progression and invasion. Our principal goals were to assess clinicopathological and prognostic correlations of microRNA-21 (miR-21) expression levels in a group of 39 Serbian breast cancer patients with invasive lobular (ILC), ductal (IDC), or mixed (ILC-IDC) breast carcinomas and in order to discover the role of miR-21 in potential novel form of stratification of the patients with different estrogen receptor (ER) and progesterone receptor (PR) status. MiR-21 expression levels were measured by stem-loop real-time RT-PCR using TaqMan technology. ER, PR, human epidermal growth factor 2 receptor (Her-2), and proliferative index (Ki-67) were evaluated by immunohistochemistry. MiR-21 levels do not vary among ILC, IDC, and ILC-IDC subgroups. MiR-21 expression levels varied significantly in the age, tumor size, Ki-67, and different grade (p = 0.030, p = 0.036, p = 0.027 and p = 0.032, respectively) subgroups. ER? and PR? showed higher miR-21 levels than their negative receptor status paired groups ER-and PR-with p = 0.012 and p = 0.018, respectively. MiR-21 positively correlated with ER and PR status (p = 0.018, rho = 0.379 and p = 0.034, rho = 0.345, respectively). Our findings suggest that miR-21 emulates transitional form of expression and that the levels of expression might be useful for stratification of the patients with different receptor status with the purpose to seek for new therapy approaches especially for the patients with the lack of response to conventional endocrine therapy.
PB  - Humana Press Inc, Totowa
T2  - Medical Oncology
T1  - Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia
VL  - 31
IS  - 6
DO  - 10.1007/s12032-014-0977-5
ER  - 

@article{
author = "Petrović, Nina and Mandusić, Vesna and Dimitrijević, Bogomir and Roganović, Jelena and Lukić, Silvana and Todorović, Lidija and Stanojević, Boban",
year = "2014",
abstract = "MicroRNAs play essential role in breast carcinoma progression and invasion. Our principal goals were to assess clinicopathological and prognostic correlations of microRNA-21 (miR-21) expression levels in a group of 39 Serbian breast cancer patients with invasive lobular (ILC), ductal (IDC), or mixed (ILC-IDC) breast carcinomas and in order to discover the role of miR-21 in potential novel form of stratification of the patients with different estrogen receptor (ER) and progesterone receptor (PR) status. MiR-21 expression levels were measured by stem-loop real-time RT-PCR using TaqMan technology. ER, PR, human epidermal growth factor 2 receptor (Her-2), and proliferative index (Ki-67) were evaluated by immunohistochemistry. MiR-21 levels do not vary among ILC, IDC, and ILC-IDC subgroups. MiR-21 expression levels varied significantly in the age, tumor size, Ki-67, and different grade (p = 0.030, p = 0.036, p = 0.027 and p = 0.032, respectively) subgroups. ER? and PR? showed higher miR-21 levels than their negative receptor status paired groups ER-and PR-with p = 0.012 and p = 0.018, respectively. MiR-21 positively correlated with ER and PR status (p = 0.018, rho = 0.379 and p = 0.034, rho = 0.345, respectively). Our findings suggest that miR-21 emulates transitional form of expression and that the levels of expression might be useful for stratification of the patients with different receptor status with the purpose to seek for new therapy approaches especially for the patients with the lack of response to conventional endocrine therapy.",
publisher = "Humana Press Inc, Totowa",
journal = "Medical Oncology",
title = "Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia",
volume = "31",
number = "6",
doi = "10.1007/s12032-014-0977-5"
}

Petrović, N., Mandusić, V., Dimitrijević, B., Roganović, J., Lukić, S., Todorović, L.,& Stanojević, B.. (2014). Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia. in Medical Oncology
Humana Press Inc, Totowa., 31(6).
https://doi.org/10.1007/s12032-014-0977-5

Petrović N, Mandusić V, Dimitrijević B, Roganović J, Lukić S, Todorović L, Stanojević B. Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia. in Medical Oncology. 2014;31(6).
doi:10.1007/s12032-014-0977-5 .

Petrović, Nina, Mandusić, Vesna, Dimitrijević, Bogomir, Roganović, Jelena, Lukić, Silvana, Todorović, Lidija, Stanojević, Boban, "Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia" in Medical Oncology, 31, no. 6 (2014),
https://doi.org/10.1007/s12032-014-0977-5 . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Mandusić, Vesna
AU  - Stanojević, Boban
AU  - Lukić, Silvana
AU  - Todorović, Lidija
AU  - Roganović, Jelena
AU  - Dimitrijević, Bogomir
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1859
AB  - MicroRNA-21 (miR-21) overexpression is characteristic for various types of tumors, but it is still unknown whether its expression levels differ between invasive and non-invasive breast carcinomas. The main goal of the study was to determine the difference in miR-21 expression among normal tissue, non-invasive, invasive with non-invasive component, and pure invasive breast cancer samples, to explain its potential role and significance in breast cancer invasiveness. The second goal was to propose miR-21 as molecular marker of breast cancer invasiveness and potential target for future anti-miR therapies for the prevention of invasion and metastasis. In order to reveal the role of miR-21 in breast cancer invasiveness, we measured miR-21 expression levels in 44 breast cancer and four normal samples by stem-loop real-time RT-PCR using TaqMan technology. Relative expression levels of miR-21 were significantly higher in invasive than in other groups (P = 0.002) and significantly higher in invasive compared with invasive with non-invasive component group in histological (P = 0.043) and nuclear grade 2 (P = 0.036), estrogen-receptor-positive (ER+) (P = 0.006), progesterone-receptor-positive (PR+) (P = 0.008), ER+ PR+ (P = 0.007), and proliferation index (Ki-67)  lt = 20 % (P = 0.036) tumors. Our findings suggest that miR-21 could be independent molecular marker of breast cancer invasiveness and potential target for future anti-miR therapies for the prevention of invasion and metastasis.
PB  - Humana Press Inc, Totowa
T2  - Medical Oncology
T1  - The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion
VL  - 31
IS  - 3
DO  - 10.1007/s12032-014-0867-x
ER  - 

@article{
author = "Petrović, Nina and Mandusić, Vesna and Stanojević, Boban and Lukić, Silvana and Todorović, Lidija and Roganović, Jelena and Dimitrijević, Bogomir",
year = "2014",
abstract = "MicroRNA-21 (miR-21) overexpression is characteristic for various types of tumors, but it is still unknown whether its expression levels differ between invasive and non-invasive breast carcinomas. The main goal of the study was to determine the difference in miR-21 expression among normal tissue, non-invasive, invasive with non-invasive component, and pure invasive breast cancer samples, to explain its potential role and significance in breast cancer invasiveness. The second goal was to propose miR-21 as molecular marker of breast cancer invasiveness and potential target for future anti-miR therapies for the prevention of invasion and metastasis. In order to reveal the role of miR-21 in breast cancer invasiveness, we measured miR-21 expression levels in 44 breast cancer and four normal samples by stem-loop real-time RT-PCR using TaqMan technology. Relative expression levels of miR-21 were significantly higher in invasive than in other groups (P = 0.002) and significantly higher in invasive compared with invasive with non-invasive component group in histological (P = 0.043) and nuclear grade 2 (P = 0.036), estrogen-receptor-positive (ER+) (P = 0.006), progesterone-receptor-positive (PR+) (P = 0.008), ER+ PR+ (P = 0.007), and proliferation index (Ki-67)  lt = 20 % (P = 0.036) tumors. Our findings suggest that miR-21 could be independent molecular marker of breast cancer invasiveness and potential target for future anti-miR therapies for the prevention of invasion and metastasis.",
publisher = "Humana Press Inc, Totowa",
journal = "Medical Oncology",
title = "The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion",
volume = "31",
number = "3",
doi = "10.1007/s12032-014-0867-x"
}

Petrović, N., Mandusić, V., Stanojević, B., Lukić, S., Todorović, L., Roganović, J.,& Dimitrijević, B.. (2014). The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion. in Medical Oncology
Humana Press Inc, Totowa., 31(3).
https://doi.org/10.1007/s12032-014-0867-x

Petrović N, Mandusić V, Stanojević B, Lukić S, Todorović L, Roganović J, Dimitrijević B. The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion. in Medical Oncology. 2014;31(3).
doi:10.1007/s12032-014-0867-x .

Petrović, Nina, Mandusić, Vesna, Stanojević, Boban, Lukić, Silvana, Todorović, Lidija, Roganović, Jelena, Dimitrijević, Bogomir, "The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion" in Medical Oncology, 31, no. 3 (2014),
https://doi.org/10.1007/s12032-014-0867-x . .

TY  - JOUR
AU  - Tanić, Nasta
AU  - Tanić, Nikola
AU  - Milašin, Jelena
AU  - Vukadinović, Miroslav
AU  - Dimitrijević, Bogomir
PY  - 2009
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1483
AB  - Leukoplakias, clinically identifiable premalignant lesions, often precede oral squamous cell carcinoma (OSCC). Identification of leukoplakias that have the potential for transformation to malignancy is a key clinical problem. The aim of this study was to assess genomic instability, and to detect tumor-specific genomic alterations, in leukoplakias. Genomic instability was analyzed by comparing the DNA fingerprints of 32 leukoplakias with those of paired normal tissue. In addition, the mutational status of the p53 gene was analyzed using polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) and polymerase chain reaction-heteroduplex DNA (PCR-HET), and the mutations were subsequently confirmed by DNA sequencing. Moderate-to-significant genomic instability was detected in all leukoplakias analysed. Nine unique amplicons, present in leukoplakias but not in normal tissue, were retrieved and successfully characterized. The p53 gene was mutated in 40.6% of patients. Four patients with moderate instability and mutated p53 developed OSCC. The data obtained in this study support and concretize the thesis that premalignant lesions possess many of the alterations found in cancer before the development of a malignant phenotype. Inactivation or mutation of the p53 tumor-suppressor might be an early event contributing to genomic instability and increasing the risk of malignant transformation.
PB  - Wiley, Hoboken
T2  - European Journal of Oral Sciences
T1  - Genomic instability and tumor-specific DNA alterations in oral leukoplakias
VL  - 117
IS  - 3
SP  - 231
EP  - 237
DO  - 10.1111/j.1600-0722.2009.00624.x
ER  - 

@article{
author = "Tanić, Nasta and Tanić, Nikola and Milašin, Jelena and Vukadinović, Miroslav and Dimitrijević, Bogomir",
year = "2009",
abstract = "Leukoplakias, clinically identifiable premalignant lesions, often precede oral squamous cell carcinoma (OSCC). Identification of leukoplakias that have the potential for transformation to malignancy is a key clinical problem. The aim of this study was to assess genomic instability, and to detect tumor-specific genomic alterations, in leukoplakias. Genomic instability was analyzed by comparing the DNA fingerprints of 32 leukoplakias with those of paired normal tissue. In addition, the mutational status of the p53 gene was analyzed using polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) and polymerase chain reaction-heteroduplex DNA (PCR-HET), and the mutations were subsequently confirmed by DNA sequencing. Moderate-to-significant genomic instability was detected in all leukoplakias analysed. Nine unique amplicons, present in leukoplakias but not in normal tissue, were retrieved and successfully characterized. The p53 gene was mutated in 40.6% of patients. Four patients with moderate instability and mutated p53 developed OSCC. The data obtained in this study support and concretize the thesis that premalignant lesions possess many of the alterations found in cancer before the development of a malignant phenotype. Inactivation or mutation of the p53 tumor-suppressor might be an early event contributing to genomic instability and increasing the risk of malignant transformation.",
publisher = "Wiley, Hoboken",
journal = "European Journal of Oral Sciences",
title = "Genomic instability and tumor-specific DNA alterations in oral leukoplakias",
volume = "117",
number = "3",
pages = "231-237",
doi = "10.1111/j.1600-0722.2009.00624.x"
}

Tanić, N., Tanić, N., Milašin, J., Vukadinović, M.,& Dimitrijević, B.. (2009). Genomic instability and tumor-specific DNA alterations in oral leukoplakias. in European Journal of Oral Sciences
Wiley, Hoboken., 117(3), 231-237.
https://doi.org/10.1111/j.1600-0722.2009.00624.x

Tanić N, Tanić N, Milašin J, Vukadinović M, Dimitrijević B. Genomic instability and tumor-specific DNA alterations in oral leukoplakias. in European Journal of Oral Sciences. 2009;117(3):231-237.
doi:10.1111/j.1600-0722.2009.00624.x .

Tanić, Nasta, Tanić, Nikola, Milašin, Jelena, Vukadinović, Miroslav, Dimitrijević, Bogomir, "Genomic instability and tumor-specific DNA alterations in oral leukoplakias" in European Journal of Oral Sciences, 117, no. 3 (2009):231-237,
https://doi.org/10.1111/j.1600-0722.2009.00624.x . .

TY  - JOUR
AU  - Ninković, Tanja
AU  - Dedović, Nasta
AU  - Kosanović, Rade
AU  - Dimitrijević, Bogomir
AU  - Josipović, Obrad
AU  - Vukadinović, Miroslav
AU  - Milašin, Jelena
PY  - 2003
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1174
AB  - Although myc genes are an important family of oncogenes involved in human carcinogenesis, data concerning its role in head and neck tumors are quite limited. Moreover, information regarding precancerous lesions is almost inexisting. In order to better understand mechanisms of progression of premalignant lesions into malignant and the possible role of c-myc amplification, the status of this oncogene was analyzed in two types of pathological changes known to possess malignant transformation potential oral leukoplakia and chronic laryngitis. In the first type of lesions a high percentage of c-myc activation (33%) was observed pointing to a probable role of myc in leukoplakia malignant transformation, while in the other type of lesion there were no signs of myc amplification.
AB  - C-myc spada u red onkogena sa važnom ulogom u humanoj kancerogenezi, međutim malo je podataka u literaturi o promenama ovog gena u malignim i premalignim lezijama glave i vrata. Sa ciljem da se bolje upoznaju mehanizmi evolucije prekanceroznih lezija u prave kancere sa naglaskom na c-myc onkogen, analiziran je njegov status u dva tipa promena za koje se zna da imaju maligni potencijal: oralnim leukoplakijama i hroničnim laringitisima. U prvom tipu lezija ustanovljen je visok stepen (33%) aktivacije myc-onkogena, što bi ukazivalo na značaj ove promene u malignoj transformaciji leukoplakija, dok u drugom nije nađen nijedan uzorak sa ovom promenom.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - C-myc amplification in precancerous lesions of oral cavity and larynx
T1  - Amplifikacija c-myc onkogena u prekancerozama usne duplje i larinksa
VL  - 50
IS  - 3
SP  - 117
EP  - 119
DO  - 10.2298/SGS0303117N
ER  - 

@article{
author = "Ninković, Tanja and Dedović, Nasta and Kosanović, Rade and Dimitrijević, Bogomir and Josipović, Obrad and Vukadinović, Miroslav and Milašin, Jelena",
year = "2003",
abstract = "Although myc genes are an important family of oncogenes involved in human carcinogenesis, data concerning its role in head and neck tumors are quite limited. Moreover, information regarding precancerous lesions is almost inexisting. In order to better understand mechanisms of progression of premalignant lesions into malignant and the possible role of c-myc amplification, the status of this oncogene was analyzed in two types of pathological changes known to possess malignant transformation potential oral leukoplakia and chronic laryngitis. In the first type of lesions a high percentage of c-myc activation (33%) was observed pointing to a probable role of myc in leukoplakia malignant transformation, while in the other type of lesion there were no signs of myc amplification., C-myc spada u red onkogena sa važnom ulogom u humanoj kancerogenezi, međutim malo je podataka u literaturi o promenama ovog gena u malignim i premalignim lezijama glave i vrata. Sa ciljem da se bolje upoznaju mehanizmi evolucije prekanceroznih lezija u prave kancere sa naglaskom na c-myc onkogen, analiziran je njegov status u dva tipa promena za koje se zna da imaju maligni potencijal: oralnim leukoplakijama i hroničnim laringitisima. U prvom tipu lezija ustanovljen je visok stepen (33%) aktivacije myc-onkogena, što bi ukazivalo na značaj ove promene u malignoj transformaciji leukoplakija, dok u drugom nije nađen nijedan uzorak sa ovom promenom.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "C-myc amplification in precancerous lesions of oral cavity and larynx, Amplifikacija c-myc onkogena u prekancerozama usne duplje i larinksa",
volume = "50",
number = "3",
pages = "117-119",
doi = "10.2298/SGS0303117N"
}

Ninković, T., Dedović, N., Kosanović, R., Dimitrijević, B., Josipović, O., Vukadinović, M.,& Milašin, J.. (2003). C-myc amplification in precancerous lesions of oral cavity and larynx. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 50(3), 117-119.
https://doi.org/10.2298/SGS0303117N

Ninković T, Dedović N, Kosanović R, Dimitrijević B, Josipović O, Vukadinović M, Milašin J. C-myc amplification in precancerous lesions of oral cavity and larynx. in Stomatološki glasnik Srbije. 2003;50(3):117-119.
doi:10.2298/SGS0303117N .

Ninković, Tanja, Dedović, Nasta, Kosanović, Rade, Dimitrijević, Bogomir, Josipović, Obrad, Vukadinović, Miroslav, Milašin, Jelena, "C-myc amplification in precancerous lesions of oral cavity and larynx" in Stomatološki glasnik Srbije, 50, no. 3 (2003):117-119,
https://doi.org/10.2298/SGS0303117N . .