TY  - JOUR
AU  - Puač, Nevena
AU  - Miletić, Maja
AU  - Mojović, Miloš
AU  - Popović-Bijelić, Ana
AU  - Vuković, Dragana
AU  - Miličić, Biljana
AU  - Maletić, Dejan
AU  - Lazović, Saša
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj.
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1966
AB  - In this paper we will present results for plasma sterilization of planktonic samples of two reference strains of bacteria, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. We have used a plasma needle as a source of non-equilibrium atmospheric plasma in all treatments. This device is already well characterized by OES, derivative probes and mass spectrometry. It was shown that power delivered to the plasma is bellow 2 W and that it produces the main radical oxygen and nitrogen species believed to be responsible for the sterilization process. Here we will only present results obtained by electron paramagnetic resonance which was used to detect the OH, H and NO species. Treatment time and power delivered to the plasma were found to have the strongest influence on sterilization. In all cases we have observed a reduction of several orders of magnitude in the concentration of bacteria and for the longest treatment time complete eradication. A more efficient sterilization was achieved in the case of gram negative bacteria.
PB  - De Gruyter Open Ltd, Warsaw
T2  - Open Chemistry
T1  - Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment
VL  - 13
IS  - 1
SP  - 332
EP  - 338
DO  - 10.1515/chem-2015-0041
ER  - 

@article{
author = "Puač, Nevena and Miletić, Maja and Mojović, Miloš and Popović-Bijelić, Ana and Vuković, Dragana and Miličić, Biljana and Maletić, Dejan and Lazović, Saša and Malović, Gordana and Petrović, Zoran Lj.",
year = "2015",
abstract = "In this paper we will present results for plasma sterilization of planktonic samples of two reference strains of bacteria, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. We have used a plasma needle as a source of non-equilibrium atmospheric plasma in all treatments. This device is already well characterized by OES, derivative probes and mass spectrometry. It was shown that power delivered to the plasma is bellow 2 W and that it produces the main radical oxygen and nitrogen species believed to be responsible for the sterilization process. Here we will only present results obtained by electron paramagnetic resonance which was used to detect the OH, H and NO species. Treatment time and power delivered to the plasma were found to have the strongest influence on sterilization. In all cases we have observed a reduction of several orders of magnitude in the concentration of bacteria and for the longest treatment time complete eradication. A more efficient sterilization was achieved in the case of gram negative bacteria.",
publisher = "De Gruyter Open Ltd, Warsaw",
journal = "Open Chemistry",
title = "Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment",
volume = "13",
number = "1",
pages = "332-338",
doi = "10.1515/chem-2015-0041"
}

Puač, N., Miletić, M., Mojović, M., Popović-Bijelić, A., Vuković, D., Miličić, B., Maletić, D., Lazović, S., Malović, G.,& Petrović, Z. Lj.. (2015). Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment. in Open Chemistry
De Gruyter Open Ltd, Warsaw., 13(1), 332-338.
https://doi.org/10.1515/chem-2015-0041

Puač N, Miletić M, Mojović M, Popović-Bijelić A, Vuković D, Miličić B, Maletić D, Lazović S, Malović G, Petrović ZL. Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment. in Open Chemistry. 2015;13(1):332-338.
doi:10.1515/chem-2015-0041 .

Puač, Nevena, Miletić, Maja, Mojović, Miloš, Popović-Bijelić, Ana, Vuković, Dragana, Miličić, Biljana, Maletić, Dejan, Lazović, Saša, Malović, Gordana, Petrović, Zoran Lj., "Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment" in Open Chemistry, 13, no. 1 (2015):332-338,
https://doi.org/10.1515/chem-2015-0041 . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Marković, Tatjana
AU  - Mojović, Miloš
AU  - Pejin, Boris
AU  - Savić, Aleksandar
AU  - Perić, Tamara
AU  - Marković, Dejan
AU  - Stević, Tatjana
AU  - Soković, Marina
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1829
AB  - The aim of this study was to examine chemical composition and biological activity of Gaultheria procumbens L essential oil (EO) against food spoilage and oral microorganisms. The components of EO were identified by GC-MS. Antimicrobial activity was determined against food spoilage (five bacteria and six fungal species) and oral microorganisms (eight bacteria and thirty two fungal species) by microdilution and microplate biofilm assay, antioxidant activity was tested using the persistent free radical 2,2-diphenyl-1-picryl hydrazyl (DPPH), while antiradical activity was examined by fluorescence spectroscopy and electron paramagnetic resonance spectroscopy (EPR). GC-MS analysis showed that methyl salicylate (96.90%) was the main component of the oil. Essential oil inhibited the growth of all microorganisms tested, i.e. food and oral bacteria and fungi, respectively (MIC 0.18-3.00 mg/ml and MBC 1.25-4.00 mg/ml; MIC 0.73-5.00 mg/ml and MFC 2.92-26.67 mg/ml); The oil effectively inhibited the biofilm formation of oral Streptococcus mutans and Candida albicans as well (MIC 25.00 MBC 50.00 mg/ml; MIC 12.50, MFC 50.00 mg/ml). In addition, oil exhibited a dose-dependent DPPH-radical-scavenging activity with IC50 value of 30.61 mg/ml. The specific fluorescence probe 2-[6-(4'-amino)phenoxy-3H-xanten-3-on-9-yl] benzoic acid (APP) and the the spin trap 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO), capable for simultaneous detection of different free radical specie were used in antiradical activity of the oil measurements. Oil showed a moderate antiradical activity, reducing quantity of produced hydroxyl radicals to about 20% of initial value. This study succeeds in creating directly comparable and quantitative data for the oil unsufficiently examined so far.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops & Products
T1  - Chemical composition and biological activity of Gaultheria procumbens L. essential oil
VL  - 49
SP  - 561
EP  - 567
DO  - 10.1016/j.indcrop.2013.06.002
ER  - 

@article{
author = "Nikolić, Miloš and Marković, Tatjana and Mojović, Miloš and Pejin, Boris and Savić, Aleksandar and Perić, Tamara and Marković, Dejan and Stević, Tatjana and Soković, Marina",
year = "2013",
abstract = "The aim of this study was to examine chemical composition and biological activity of Gaultheria procumbens L essential oil (EO) against food spoilage and oral microorganisms. The components of EO were identified by GC-MS. Antimicrobial activity was determined against food spoilage (five bacteria and six fungal species) and oral microorganisms (eight bacteria and thirty two fungal species) by microdilution and microplate biofilm assay, antioxidant activity was tested using the persistent free radical 2,2-diphenyl-1-picryl hydrazyl (DPPH), while antiradical activity was examined by fluorescence spectroscopy and electron paramagnetic resonance spectroscopy (EPR). GC-MS analysis showed that methyl salicylate (96.90%) was the main component of the oil. Essential oil inhibited the growth of all microorganisms tested, i.e. food and oral bacteria and fungi, respectively (MIC 0.18-3.00 mg/ml and MBC 1.25-4.00 mg/ml; MIC 0.73-5.00 mg/ml and MFC 2.92-26.67 mg/ml); The oil effectively inhibited the biofilm formation of oral Streptococcus mutans and Candida albicans as well (MIC 25.00 MBC 50.00 mg/ml; MIC 12.50, MFC 50.00 mg/ml). In addition, oil exhibited a dose-dependent DPPH-radical-scavenging activity with IC50 value of 30.61 mg/ml. The specific fluorescence probe 2-[6-(4'-amino)phenoxy-3H-xanten-3-on-9-yl] benzoic acid (APP) and the the spin trap 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO), capable for simultaneous detection of different free radical specie were used in antiradical activity of the oil measurements. Oil showed a moderate antiradical activity, reducing quantity of produced hydroxyl radicals to about 20% of initial value. This study succeeds in creating directly comparable and quantitative data for the oil unsufficiently examined so far.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops & Products",
title = "Chemical composition and biological activity of Gaultheria procumbens L. essential oil",
volume = "49",
pages = "561-567",
doi = "10.1016/j.indcrop.2013.06.002"
}

Nikolić, M., Marković, T., Mojović, M., Pejin, B., Savić, A., Perić, T., Marković, D., Stević, T.,& Soković, M.. (2013). Chemical composition and biological activity of Gaultheria procumbens L. essential oil. in Industrial Crops & Products
Elsevier, Amsterdam., 49, 561-567.
https://doi.org/10.1016/j.indcrop.2013.06.002

Nikolić M, Marković T, Mojović M, Pejin B, Savić A, Perić T, Marković D, Stević T, Soković M. Chemical composition and biological activity of Gaultheria procumbens L. essential oil. in Industrial Crops & Products. 2013;49:561-567.
doi:10.1016/j.indcrop.2013.06.002 .

Nikolić, Miloš, Marković, Tatjana, Mojović, Miloš, Pejin, Boris, Savić, Aleksandar, Perić, Tamara, Marković, Dejan, Stević, Tatjana, Soković, Marina, "Chemical composition and biological activity of Gaultheria procumbens L. essential oil" in Industrial Crops & Products, 49 (2013):561-567,
https://doi.org/10.1016/j.indcrop.2013.06.002 . .