TY  - JOUR
AU  - Trišić, Dijana
AU  - Ćetenović, Bojana
AU  - Zdravković, Nemanja
AU  - Marković, Tatjana
AU  - Dojčinović, Biljana
AU  - Jokanović, Vukoman
AU  - Marković, Dejan
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2444
AB  - Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation. The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA +) and UltraCal XS (CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Entero-coccus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the an-tibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA + had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA + in 3h and 24h of the incubation period (p  lt  0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA +. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTA substitution.
AB  - Uvod/Cilj. Osnovni cilj endodonskog lečenja je eliminacija patološki izmenjenog tkiva, eliminacija infekcije korensko kanala i njegovo hermetičko trodimenzionalno zatvaranje. Cilj istraživanja je bio da se proceni antibakterijska aktivnost novih endodontskih nano-strukturiranih materijala na bazi visoko aktivnih kalcijum silikata albo-mineral polyoxide carbonate aggregate (ALBO-MPCA) i calcium silicates (CS) u odnosu na mineral trioxide aggregate (MTA +) i UltraCal XS (CH). Metode. Testirana je antibakterijska aktivnost materijala protiv Staphylococcus aureus (ATCC 25923) i Enterococcus faecalis (ATCC 14506), kao i kliničkih izolata: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus i Streptococcus vestibularis pomoću agar difuzionog testa. Merenja pH vred-nosti obavljena su korišćenjem pH metra. Ukupan iznos oslobođenih jona određivan je pomoću ICP-OES. Rezultati. Svi testirani materijali pokazali su najbolji antibakterijski efekat nakon 1 h od inkubacije. Nakon 3 h i 24 h od inkubacije, antibakterijski efekat svih testiranih materijala bio je sličan. Agar difuzioni test pokazao je da materijali ALBOMPCA, CS i MTA+ ispoljavaju slične zone inhibicije rasta (p > 0.05) osim u slučaju Staphylococcus aureus, gde je materijal ALBO-MPCA pokazao bolje antibakterijsko dejstvo nego MTA+ nakon 3 h i 24 h od inkubacije (p  lt  0.05). Nakon 24 h od inkubacije, zone inhibicije su bile najizraženije u slučaju materijala CS protiv Staphylococcus aureus (16.67 ± 2.34 mm), zatim ALBO-MPCA (14.67 ± 1.21 mm), a najslabije u slučaju CS protiv Enterococcus faecalis (6.50±1.76 mm). Materijal CH ispoljio je najveću pH vrednost, zatim ALBOMPCA, CS i MTA+. Zaključak. Ispoljeni antibakterijski efekti ukazuju na to da materijali na bazi visoko aktivnih kalcijum silikata mogu da predstavljaju efikasnu zamenu za MTA u terapiji zuba sa nezavršenim rastom korena u jednoj poseti, te se stoga preporučuju za dalja klinička ispitivanja
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Antibacterial effects of new endodontic materials based on calcium silicates
T1  - Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata
VL  - 76
IS  - 4
SP  - 365
EP  - 372
DO  - 10.2298/VSP161231130T
ER  - 

@article{
author = "Trišić, Dijana and Ćetenović, Bojana and Zdravković, Nemanja and Marković, Tatjana and Dojčinović, Biljana and Jokanović, Vukoman and Marković, Dejan",
year = "2019",
abstract = "Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation. The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA +) and UltraCal XS (CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Entero-coccus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the an-tibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA + had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA + in 3h and 24h of the incubation period (p  lt  0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA +. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTA substitution., Uvod/Cilj. Osnovni cilj endodonskog lečenja je eliminacija patološki izmenjenog tkiva, eliminacija infekcije korensko kanala i njegovo hermetičko trodimenzionalno zatvaranje. Cilj istraživanja je bio da se proceni antibakterijska aktivnost novih endodontskih nano-strukturiranih materijala na bazi visoko aktivnih kalcijum silikata albo-mineral polyoxide carbonate aggregate (ALBO-MPCA) i calcium silicates (CS) u odnosu na mineral trioxide aggregate (MTA +) i UltraCal XS (CH). Metode. Testirana je antibakterijska aktivnost materijala protiv Staphylococcus aureus (ATCC 25923) i Enterococcus faecalis (ATCC 14506), kao i kliničkih izolata: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus i Streptococcus vestibularis pomoću agar difuzionog testa. Merenja pH vred-nosti obavljena su korišćenjem pH metra. Ukupan iznos oslobođenih jona određivan je pomoću ICP-OES. Rezultati. Svi testirani materijali pokazali su najbolji antibakterijski efekat nakon 1 h od inkubacije. Nakon 3 h i 24 h od inkubacije, antibakterijski efekat svih testiranih materijala bio je sličan. Agar difuzioni test pokazao je da materijali ALBOMPCA, CS i MTA+ ispoljavaju slične zone inhibicije rasta (p > 0.05) osim u slučaju Staphylococcus aureus, gde je materijal ALBO-MPCA pokazao bolje antibakterijsko dejstvo nego MTA+ nakon 3 h i 24 h od inkubacije (p  lt  0.05). Nakon 24 h od inkubacije, zone inhibicije su bile najizraženije u slučaju materijala CS protiv Staphylococcus aureus (16.67 ± 2.34 mm), zatim ALBO-MPCA (14.67 ± 1.21 mm), a najslabije u slučaju CS protiv Enterococcus faecalis (6.50±1.76 mm). Materijal CH ispoljio je najveću pH vrednost, zatim ALBOMPCA, CS i MTA+. Zaključak. Ispoljeni antibakterijski efekti ukazuju na to da materijali na bazi visoko aktivnih kalcijum silikata mogu da predstavljaju efikasnu zamenu za MTA u terapiji zuba sa nezavršenim rastom korena u jednoj poseti, te se stoga preporučuju za dalja klinička ispitivanja",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Antibacterial effects of new endodontic materials based on calcium silicates, Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata",
volume = "76",
number = "4",
pages = "365-372",
doi = "10.2298/VSP161231130T"
}

Trišić, D., Ćetenović, B., Zdravković, N., Marković, T., Dojčinović, B., Jokanović, V.,& Marković, D.. (2019). Antibacterial effects of new endodontic materials based on calcium silicates. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 76(4), 365-372.
https://doi.org/10.2298/VSP161231130T

Trišić D, Ćetenović B, Zdravković N, Marković T, Dojčinović B, Jokanović V, Marković D. Antibacterial effects of new endodontic materials based on calcium silicates. in Vojnosanitetski pregled. 2019;76(4):365-372.
doi:10.2298/VSP161231130T .

Trišić, Dijana, Ćetenović, Bojana, Zdravković, Nemanja, Marković, Tatjana, Dojčinović, Biljana, Jokanović, Vukoman, Marković, Dejan, "Antibacterial effects of new endodontic materials based on calcium silicates" in Vojnosanitetski pregled, 76, no. 4 (2019):365-372,
https://doi.org/10.2298/VSP161231130T . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Jovanović, Katarina K.
AU  - Marković, Tatjana
AU  - Marković, Dejan
AU  - Gligorijević, Nevenka
AU  - Radulović, Siniša
AU  - Kostić, Marina
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2227
AB  - Objectives The chemical composition, antimicrobial and synergistic effect, and cytotoxic activity of Citrus limon (lemon), Piper nigrum (green pepper) and Melaleuca alternifoila (tea tree) essential oils (EOs) were investigated. Methods Chemical analyses of essential oils were tested by GC-FID and GC-MS spectroscopy. The antimicrobial activity assay was conducted using microdilution method against several oral bacteria and Candida spp. originating from the humans with oral disorders. The synergistic antimicrobial activity was evaluated using checkerboard method. The cytotoxicity evaluation of EOs was assessed using MTT test. Key findings Limonene (37.5%) and beta-pinene (17.9%) were the major compounds in C. limon oil, -pinene (34.4%), delta-3-carene (19.7%), limonene (18.7%) and alpha-pinene (10.4%) in P. nigrum oil and terpinen-4-ol (38.6%) and gamma-terpinene (21.7%) in M. alternifolia oil. The broad-spectrum antimicrobial activity was achieved by tested three EOs, with C. limon oil being the strongest against bacteria and M. alternifolia oil strongest against fungi. The EOs demonstrated synergism; their combined application revealed an increase in antimicrobial activity. All tested essential oils showed lower cytotoxic activity in comparison with the positive control, and the obtained results confirmed a dose-dependent activity. Conclusions The results of this study encourage use of tested EOs in development of a novel agent intended for prevention or therapy of corresponding oral disorders.
PB  - Wiley, Hoboken
T2  - Journal of Pharmacy & Pharmacology
T1  - Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils
VL  - 69
IS  - 11
SP  - 1606
EP  - 1614
DO  - 10.1111/jphp.12792
ER  - 

@article{
author = "Nikolić, Miloš and Jovanović, Katarina K. and Marković, Tatjana and Marković, Dejan and Gligorijević, Nevenka and Radulović, Siniša and Kostić, Marina and Glamočlija, Jasmina and Soković, Marina",
year = "2017",
abstract = "Objectives The chemical composition, antimicrobial and synergistic effect, and cytotoxic activity of Citrus limon (lemon), Piper nigrum (green pepper) and Melaleuca alternifoila (tea tree) essential oils (EOs) were investigated. Methods Chemical analyses of essential oils were tested by GC-FID and GC-MS spectroscopy. The antimicrobial activity assay was conducted using microdilution method against several oral bacteria and Candida spp. originating from the humans with oral disorders. The synergistic antimicrobial activity was evaluated using checkerboard method. The cytotoxicity evaluation of EOs was assessed using MTT test. Key findings Limonene (37.5%) and beta-pinene (17.9%) were the major compounds in C. limon oil, -pinene (34.4%), delta-3-carene (19.7%), limonene (18.7%) and alpha-pinene (10.4%) in P. nigrum oil and terpinen-4-ol (38.6%) and gamma-terpinene (21.7%) in M. alternifolia oil. The broad-spectrum antimicrobial activity was achieved by tested three EOs, with C. limon oil being the strongest against bacteria and M. alternifolia oil strongest against fungi. The EOs demonstrated synergism; their combined application revealed an increase in antimicrobial activity. All tested essential oils showed lower cytotoxic activity in comparison with the positive control, and the obtained results confirmed a dose-dependent activity. Conclusions The results of this study encourage use of tested EOs in development of a novel agent intended for prevention or therapy of corresponding oral disorders.",
publisher = "Wiley, Hoboken",
journal = "Journal of Pharmacy & Pharmacology",
title = "Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils",
volume = "69",
number = "11",
pages = "1606-1614",
doi = "10.1111/jphp.12792"
}

Nikolić, M., Jovanović, K. K., Marković, T., Marković, D., Gligorijević, N., Radulović, S., Kostić, M., Glamočlija, J.,& Soković, M.. (2017). Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils. in Journal of Pharmacy & Pharmacology
Wiley, Hoboken., 69(11), 1606-1614.
https://doi.org/10.1111/jphp.12792

Nikolić M, Jovanović KK, Marković T, Marković D, Gligorijević N, Radulović S, Kostić M, Glamočlija J, Soković M. Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils. in Journal of Pharmacy & Pharmacology. 2017;69(11):1606-1614.
doi:10.1111/jphp.12792 .

Nikolić, Miloš, Jovanović, Katarina K., Marković, Tatjana, Marković, Dejan, Gligorijević, Nevenka, Radulović, Siniša, Kostić, Marina, Glamočlija, Jasmina, Soković, Marina, "Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils" in Journal of Pharmacy & Pharmacology, 69, no. 11 (2017):1606-1614,
https://doi.org/10.1111/jphp.12792 . .

TY  - CHAP
AU  - Marković, Dejan
AU  - Ćetenović, Bojana
AU  - Vuković, Ana
AU  - Jokanović, Vukoman
AU  - Marković, Tatjana
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2154
AB  - Calcium-silicate-based materials are used in many endodontic and oral-surgical procedures. The advantages of calcium-silicate-based materials are good apical sealing, setting in the presence of moisture, and acquiring high pH after mixing. However, these materials also have some disadvantages: low flowability, dry consistency, and long setting time. By applying nanotechnology during synthesis, very active calcium silicate, such as nanostructured mineral polyoxide carbonate aggregate, may be used to overcome the above-mentioned disadvantages and further enhance physical properties of the reaction mixture consisting of calcite and monoclinic bismuth oxide. Application of the sol-gel method for calcite production and combination of such a method with high-temperature, self-propagating synthesis of calcium silicates makes the strategy completely innovative. Materials based on nanostructured calcium silicates may represent effective therapeutic agents for root canal obturation, especially in the case of immature roots. The application of calcium-silicate-based materials may significantly decrease the duration of therapy, reduce the risk of tooth fractures, and overcome incomplete calcification problem.
PB  - Elsevier Inc.
T2  - Nanobiomaterials in Dentistry: Applications of Nanobiomaterials
T1  - Nanosynthesized calcium-silicate-based biomaterials in endodontic treatment of young permanent teeth
VL  - 11
SP  - 269
EP  - 307
DO  - 10.1016/B978-0-323-42867-5.00011-4
ER  - 

@inbook{
author = "Marković, Dejan and Ćetenović, Bojana and Vuković, Ana and Jokanović, Vukoman and Marković, Tatjana",
year = "2016",
abstract = "Calcium-silicate-based materials are used in many endodontic and oral-surgical procedures. The advantages of calcium-silicate-based materials are good apical sealing, setting in the presence of moisture, and acquiring high pH after mixing. However, these materials also have some disadvantages: low flowability, dry consistency, and long setting time. By applying nanotechnology during synthesis, very active calcium silicate, such as nanostructured mineral polyoxide carbonate aggregate, may be used to overcome the above-mentioned disadvantages and further enhance physical properties of the reaction mixture consisting of calcite and monoclinic bismuth oxide. Application of the sol-gel method for calcite production and combination of such a method with high-temperature, self-propagating synthesis of calcium silicates makes the strategy completely innovative. Materials based on nanostructured calcium silicates may represent effective therapeutic agents for root canal obturation, especially in the case of immature roots. The application of calcium-silicate-based materials may significantly decrease the duration of therapy, reduce the risk of tooth fractures, and overcome incomplete calcification problem.",
publisher = "Elsevier Inc.",
journal = "Nanobiomaterials in Dentistry: Applications of Nanobiomaterials",
booktitle = "Nanosynthesized calcium-silicate-based biomaterials in endodontic treatment of young permanent teeth",
volume = "11",
pages = "269-307",
doi = "10.1016/B978-0-323-42867-5.00011-4"
}

Marković, D., Ćetenović, B., Vuković, A., Jokanović, V.,& Marković, T.. (2016). Nanosynthesized calcium-silicate-based biomaterials in endodontic treatment of young permanent teeth. in Nanobiomaterials in Dentistry: Applications of Nanobiomaterials
Elsevier Inc.., 11, 269-307.
https://doi.org/10.1016/B978-0-323-42867-5.00011-4

Marković D, Ćetenović B, Vuković A, Jokanović V, Marković T. Nanosynthesized calcium-silicate-based biomaterials in endodontic treatment of young permanent teeth. in Nanobiomaterials in Dentistry: Applications of Nanobiomaterials. 2016;11:269-307.
doi:10.1016/B978-0-323-42867-5.00011-4 .

Marković, Dejan, Ćetenović, Bojana, Vuković, Ana, Jokanović, Vukoman, Marković, Tatjana, "Nanosynthesized calcium-silicate-based biomaterials in endodontic treatment of young permanent teeth" in Nanobiomaterials in Dentistry: Applications of Nanobiomaterials, 11 (2016):269-307,
https://doi.org/10.1016/B978-0-323-42867-5.00011-4 . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Smiljković, Marija
AU  - Marković, Tatjana
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Marković, Dejan
AU  - Soković, Marina
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2171
AB  - The aim of this study was to investigate the chemical composition and antifungal activity of four commercial essential oils from the Burseraceae family - two Boswellia carterii Flueck oils, Canarium luzonicum (Blume) A. Gray oil, and Commiphora myrrha (Nees) Engl oil, against most common Candida spp. recovered from the human oral cavity. The essential oil samples were analyzed by GC- FID and GC/MS. The analysis showed that major essential oils' components were. - pinene (23.04 % and 31.84 %), limonene (45.62 %) and curzerene (34.65 %), respectively. Minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations were determined using a microdilution standardized technique. All tested Candida spp. clinical isolates and ATCC strains showed susceptibility to tested essential oils in a dose dependent manner. The strongest antifungal activity was shown by essential oil of B. carterii, sample 2; the average MIC values ranged from 1.25 to 1.34 mg/ml, and MFC values ranged from 2.50 to 3.75 mg/ml, depending on the fungus. This study supports the possible use of essential oils from the Bursecaceae family in reduction and elimination of Candida spp. populations in patients with oral cavity fungal infections.
PB  - Leibniz Research Centre for Working Environment and Human Factors
T2  - EXCLI Journal
T1  - Sensitivity of clinical isolates of candida to essential oils from burseraceae family
VL  - 15
SP  - 280
EP  - 289
DO  - 10.17179/excli2014-621
ER  - 

@article{
author = "Nikolić, Miloš and Smiljković, Marija and Marković, Tatjana and Ćirić, Ana and Glamočlija, Jasmina and Marković, Dejan and Soković, Marina",
year = "2016",
abstract = "The aim of this study was to investigate the chemical composition and antifungal activity of four commercial essential oils from the Burseraceae family - two Boswellia carterii Flueck oils, Canarium luzonicum (Blume) A. Gray oil, and Commiphora myrrha (Nees) Engl oil, against most common Candida spp. recovered from the human oral cavity. The essential oil samples were analyzed by GC- FID and GC/MS. The analysis showed that major essential oils' components were. - pinene (23.04 % and 31.84 %), limonene (45.62 %) and curzerene (34.65 %), respectively. Minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations were determined using a microdilution standardized technique. All tested Candida spp. clinical isolates and ATCC strains showed susceptibility to tested essential oils in a dose dependent manner. The strongest antifungal activity was shown by essential oil of B. carterii, sample 2; the average MIC values ranged from 1.25 to 1.34 mg/ml, and MFC values ranged from 2.50 to 3.75 mg/ml, depending on the fungus. This study supports the possible use of essential oils from the Bursecaceae family in reduction and elimination of Candida spp. populations in patients with oral cavity fungal infections.",
publisher = "Leibniz Research Centre for Working Environment and Human Factors",
journal = "EXCLI Journal",
title = "Sensitivity of clinical isolates of candida to essential oils from burseraceae family",
volume = "15",
pages = "280-289",
doi = "10.17179/excli2014-621"
}

Nikolić, M., Smiljković, M., Marković, T., Ćirić, A., Glamočlija, J., Marković, D.,& Soković, M.. (2016). Sensitivity of clinical isolates of candida to essential oils from burseraceae family. in EXCLI Journal
Leibniz Research Centre for Working Environment and Human Factors., 15, 280-289.
https://doi.org/10.17179/excli2014-621

Nikolić M, Smiljković M, Marković T, Ćirić A, Glamočlija J, Marković D, Soković M. Sensitivity of clinical isolates of candida to essential oils from burseraceae family. in EXCLI Journal. 2016;15:280-289.
doi:10.17179/excli2014-621 .

Nikolić, Miloš, Smiljković, Marija, Marković, Tatjana, Ćirić, Ana, Glamočlija, Jasmina, Marković, Dejan, Soković, Marina, "Sensitivity of clinical isolates of candida to essential oils from burseraceae family" in EXCLI Journal, 15 (2016):280-289,
https://doi.org/10.17179/excli2014-621 . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Marković, Tatjana
AU  - Marković, Dejan
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Smiljković, Marija
AU  - Soković, Marina
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2165
AB  - Chemical composition, antimicrobial and cytotoxic activities of commercial essential oils ' samples from the aerial plant parts of H. officinalis, R. officinalis and S. officinalis were investigated. Analyses by GC-FID and GC-MS confirmed 52 oil components. The major constituent of the H. officinalis oil was cis-pinocamphone (34.4%), followed by trans- pinocamphone (23.3%), and β-pinene (11.3%). Analysis of R. officinalis oil revealed 1.8-cineol as a major constituent (43.8%), as well as trans- pinocamphone (12.5%), α-pinene (11.5%) and β-pinene (8.2%). The most dominant constituent of S. officinalis oil was cis-thujone (32.7%), in addition to camphor (17.2%), 1.8-cineol (10.1%), α-pinene (8.6%), trans- thujone (7.7%) and camphene (7.3%). The essential oil antimicrobial activity assay was performed by the use of microdilution method against oral Candida spp. and bacteria, the major causative agents of a number of human oral disorders; all of them were susceptible to tested concentrations of H. officinalis, R. officinalis and S. officinalis essential oils, although the oil of S. officinalis exhibited the lowest antimicrobial potential. The results obtained in this study encourage use of investigated essential oils from Lamiaceae family in development of safe natural agents for prevention and/ or alternative therapy of human oral diseases. However, a special care during development of an effective natural preparation is required.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Dental Medicine
T1  - Antimicrobial activity of three Lamiaceae essential oils against common oral pathogens
VL  - 20
IS  - 3
SP  - 160
EP  - 167
DO  - 10.1515/bjdm-2016-0026
ER  - 

@article{
author = "Nikolić, Miloš and Marković, Tatjana and Marković, Dejan and Glamočlija, Jasmina and Ćirić, Ana and Smiljković, Marija and Soković, Marina",
year = "2016",
abstract = "Chemical composition, antimicrobial and cytotoxic activities of commercial essential oils ' samples from the aerial plant parts of H. officinalis, R. officinalis and S. officinalis were investigated. Analyses by GC-FID and GC-MS confirmed 52 oil components. The major constituent of the H. officinalis oil was cis-pinocamphone (34.4%), followed by trans- pinocamphone (23.3%), and β-pinene (11.3%). Analysis of R. officinalis oil revealed 1.8-cineol as a major constituent (43.8%), as well as trans- pinocamphone (12.5%), α-pinene (11.5%) and β-pinene (8.2%). The most dominant constituent of S. officinalis oil was cis-thujone (32.7%), in addition to camphor (17.2%), 1.8-cineol (10.1%), α-pinene (8.6%), trans- thujone (7.7%) and camphene (7.3%). The essential oil antimicrobial activity assay was performed by the use of microdilution method against oral Candida spp. and bacteria, the major causative agents of a number of human oral disorders; all of them were susceptible to tested concentrations of H. officinalis, R. officinalis and S. officinalis essential oils, although the oil of S. officinalis exhibited the lowest antimicrobial potential. The results obtained in this study encourage use of investigated essential oils from Lamiaceae family in development of safe natural agents for prevention and/ or alternative therapy of human oral diseases. However, a special care during development of an effective natural preparation is required.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Dental Medicine",
title = "Antimicrobial activity of three Lamiaceae essential oils against common oral pathogens",
volume = "20",
number = "3",
pages = "160-167",
doi = "10.1515/bjdm-2016-0026"
}

Nikolić, M., Marković, T., Marković, D., Glamočlija, J., Ćirić, A., Smiljković, M.,& Soković, M.. (2016). Antimicrobial activity of three Lamiaceae essential oils against common oral pathogens. in Balkan Journal of Dental Medicine
Udruženje stomatologa Balkana., 20(3), 160-167.
https://doi.org/10.1515/bjdm-2016-0026

Nikolić M, Marković T, Marković D, Glamočlija J, Ćirić A, Smiljković M, Soković M. Antimicrobial activity of three Lamiaceae essential oils against common oral pathogens. in Balkan Journal of Dental Medicine. 2016;20(3):160-167.
doi:10.1515/bjdm-2016-0026 .

Nikolić, Miloš, Marković, Tatjana, Marković, Dejan, Glamočlija, Jasmina, Ćirić, Ana, Smiljković, Marija, Soković, Marina, "Antimicrobial activity of three Lamiaceae essential oils against common oral pathogens" in Balkan Journal of Dental Medicine, 20, no. 3 (2016):160-167,
https://doi.org/10.1515/bjdm-2016-0026 . .

TY  - JOUR
AU  - Bogojević, Jelena
AU  - Nikolić, Miloš
AU  - Marković, Tatjana
AU  - Ćirić, Ana
AU  - Marković, Dejan
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2148
AB  - Twenty one essential oils (EOs) documented their significant antimicrobial effect with regard to our pre-set criterion of the Minimal Inhibitory Concentration (MIC ≤ 200 μL / mL) of EOs towards Enterococcus faecalis (ATCC 29212 and or clinical isolates); the best effect MIC 0.4 μL / mL (approx. 0.26 μg / mL) achieved Satureja horvatii L. EO, while the EOs with the lowest antimicrobial efficacy were Rosmarinus officinalis L. and Achilea milefolium L., both with MIC s160.0 μg / mL. Analysis of the MIC values within the groups revealed that ATCC strain of E. faecalis is generally lower, ranging from 0.26 to 156 μg / mL, in comparison to those for clinical isolateswhich ranged from 10 to 160 μg / mL. Twelve 12 components that are common in EOs whith MIC s ≤ 200 μg / mL in testings towards both, the clinical and referent strains are given in descending order according to number of oils they are present in: trans-β-caryophyllene (13) > myrcene (8) > α-pinene (8) > linalool (7) > p-cymene (7) > borneol (7) > geraniol (6) > camphene (6) > limonene (5) > 1,8- cineol (5) > γ -terpinene (5) > α- terpinene (4). Comparison of EO constituents reviled that only, geraniol and 1,8-cineol, contributed with ≥ 10 % to more than one EO (MIC 0.3-200 μg / mL) efficient against both E. faecalis strains. Thirteen components in 11 EOs with MIC ≤ 200 μg / mL towards ATCC 29212 were representative based on their contents in EOs: eugenol 82.9 % > thymol 63.7 % > hexadecanoic acid 47.8 % > menthol 46.6 % > cis-b-ocimene 44.2 % > geranial 42.1 % > trans-β-caryophyllene 40.8 % > citronellal 36.7 % > α-pinene 31.2 % > neral 30.5 % > α-eudesmol 22.4 % > citronellol 13.1 % > menthone 11.3 %. Following seven components, representative in 10 EOs with MIC ≤ 200 μg / mL towards clinical isolates, are presented in order of their contribution to EOs: phenylethyl alcohol 57.7 % > geranial 32.9 % > neral 22.2 % > p- cymene 20 % > carvacrol 14 % > α-pinene 11.5 % > linalool 11.4 %. Out of 21 highly efficient EOs selected in this study, six EOs proved to be the most efficient (MIC ≤ 30 μg / mL ); three oils in control of E.faecalis ATCC strain (Satureja horvatii, Mentha pulegium and Rosmarinus officinalis) and other three in control of E. faecalis clinical isolates (Leptospermum petersonii, Thymus algeriensis, Thymus serpyllum). Thymol is a major component in three out of the six aforementioned most efficient EOs. The aim of our study was to investigate differences in efficacy of selected EOs that proved to possess great antimicrobial activity, towards the referent strain ATCC 29212 and clinical isolates of E. faecalis on, and to estimate which of their constituents might contribute to desired activity, as 'markers compunds'.
AB  - Pregledom literature odabrano je 21 etarsko ulje (EU) koje pokazuje izuzetno značajan antimikrobni efekat prema kriterijumima koje smo zadali (MIC ≤ 200 μL / mL) na Enterococcus faecalis (ATCC 29212 referentni soj ili klinički izolat); Najbolji antimikrobni efekat sa MIC 0.4 μL / mL (približno 0.26 μg / mL) pokazalo je etarsko ulje Satureja horvatii L., dok su sa najslabijim antimikrobnim efektom bila ulja Rosmarinus officinalis L. i Achilea milefolium L. (MIC 160.0 μg / mL). Međugrupnom analizom MIC vrednosti, utvrđeno je da se MIC vrednosti, i u grupi u kojoj su testirana ulja na referentne sojeve i u grupi na kliničkim izolatima, razlikuju. MIC vrednosti ulja koja su delovala na referentni soj E. faecalis ATCC 29212, variraju od 0.26 to 156 μg / mL , dok je opseg MIC vrednosti koja pokazuju ulja efikasna na kliničke izolate E. faecalisa bio između 10 to 160 μg / mL. Dvanaest komponenti koje su zajedničke za etarska ulja testirana i na referentnom soju i na kliničkim izolatima, a čiji MIC ≤ 200 μg / mL , iskazana su u opadajućem nizu u odnosu na broj ulja u kojima su sadržana: trans-β-kariofilen (13) > mircen (8) > α-pinen (8) > linalool (7) > p-cymen (7) > borneol (7) > geraniol (6) > kamfen (6) > limonen (5) > 1,8-cineol (5) > γ-terpinen (5) > α-terpinen (4). Poređenjem komponenti utvrđeno je da su samo dve komponente, geraniol i 1,8-cineol, prisutne u procentualnoj zastupljenosti ≥ 10 % u više od jednog etarskog ulja (MIC opseg ulja 0.3-200 μg / mL ), a koja su pokazala efikasnost i na ATCC i na kliničke sojeve E. faecalis. Geraniol i 1,8-cineol su glavne komponente sadržaja ≥ 10 % u više ulja efikasnih i protiv ATCC soja i kliničkih izolata (MIC 0.3-200 μg / mL). U 11 ulja sa MIC ≤ 200 μg / mL na E. faecalis ATCC 29212, uočeno je 13 reprezentativnih komponenti, predstavljanih opadajućem nizu u odnosu na zastupljenost u uljima: eugenol 82.9 % > timol 63.7 % > heksadekanoanska kiselina 47.8 % > mentol 46.6 % > cis-b-ocimen 44.2 % > geranial 42.1 % > trans-β-kariofilen 40.8 % > citronelal 36.7 % > α-pinen 31.2 % > neral 30.5 % > α-eudesmol 22.4 % > citronelol 13.1 % > menton 11.3 %. U 10 etarskih ulja sa MIC ≤ 200 μg / mL efikasnim na klinički izolat, 7 je reprezentativnih i one su prikazane u opadajućem nizu u odnosu na njihovu zastupljenost u EU: feniletil alkohol 57.7 % > geranial 32.9 % > neral 22.2 % > p-cimen 20 % > karvakrol, 14 % > α-pinen 11.5 % > linalool 11.4 %. Od 21 EU iz ovog pregleda, šest su se izdvojila kao najefikasnija (MIC ≤ 30 μg / mL); 3 na referentni E. faecalis ATCC soj (Satureja horvatii, Mentha pulegium and Rosmarinus officinalis) i druga 3 na E faecalis klinički izolat (Leptospermum petersonii, Thymus algeriensis, Thymus serpyllum). Timol je bio procentualno najzastupljenija komponenta u 3 od 6 najefikasnijih EU. Cilj ovog istraživanja bio je da se utvrde razlike u efikasnosti etarskih ulja koja su odabrana zbog njihove jake antimikrobne aktivnosti na kliničke izolate E. faecalis kao i na referentni soj ATCC 29212, i da se izvrši procena njihovog hemijskog sastava vezano za komponente koje svojim učešćem doprinose antimikrobnoj aktivnosti i mogu poslužiti kao 'markeri efikasnosti'.
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Analysis of chemical composition of the most efficient essential oils towards Enterococcus faecalis referent strain ATCC 29212 and clinical isolates
T1  - Analiza hemijskog sastava etarskih ulja snažne efikasnosti na referentni soj ATCC 29212 i kliničke izolate Enterococcus faecalis
IS  - 36
SP  - 3
EP  - 25
DO  - 10.5937/leksir1636003B
ER  - 

@article{
author = "Bogojević, Jelena and Nikolić, Miloš and Marković, Tatjana and Ćirić, Ana and Marković, Dejan",
year = "2016",
abstract = "Twenty one essential oils (EOs) documented their significant antimicrobial effect with regard to our pre-set criterion of the Minimal Inhibitory Concentration (MIC ≤ 200 μL / mL) of EOs towards Enterococcus faecalis (ATCC 29212 and or clinical isolates); the best effect MIC 0.4 μL / mL (approx. 0.26 μg / mL) achieved Satureja horvatii L. EO, while the EOs with the lowest antimicrobial efficacy were Rosmarinus officinalis L. and Achilea milefolium L., both with MIC s160.0 μg / mL. Analysis of the MIC values within the groups revealed that ATCC strain of E. faecalis is generally lower, ranging from 0.26 to 156 μg / mL, in comparison to those for clinical isolateswhich ranged from 10 to 160 μg / mL. Twelve 12 components that are common in EOs whith MIC s ≤ 200 μg / mL in testings towards both, the clinical and referent strains are given in descending order according to number of oils they are present in: trans-β-caryophyllene (13) > myrcene (8) > α-pinene (8) > linalool (7) > p-cymene (7) > borneol (7) > geraniol (6) > camphene (6) > limonene (5) > 1,8- cineol (5) > γ -terpinene (5) > α- terpinene (4). Comparison of EO constituents reviled that only, geraniol and 1,8-cineol, contributed with ≥ 10 % to more than one EO (MIC 0.3-200 μg / mL) efficient against both E. faecalis strains. Thirteen components in 11 EOs with MIC ≤ 200 μg / mL towards ATCC 29212 were representative based on their contents in EOs: eugenol 82.9 % > thymol 63.7 % > hexadecanoic acid 47.8 % > menthol 46.6 % > cis-b-ocimene 44.2 % > geranial 42.1 % > trans-β-caryophyllene 40.8 % > citronellal 36.7 % > α-pinene 31.2 % > neral 30.5 % > α-eudesmol 22.4 % > citronellol 13.1 % > menthone 11.3 %. Following seven components, representative in 10 EOs with MIC ≤ 200 μg / mL towards clinical isolates, are presented in order of their contribution to EOs: phenylethyl alcohol 57.7 % > geranial 32.9 % > neral 22.2 % > p- cymene 20 % > carvacrol 14 % > α-pinene 11.5 % > linalool 11.4 %. Out of 21 highly efficient EOs selected in this study, six EOs proved to be the most efficient (MIC ≤ 30 μg / mL ); three oils in control of E.faecalis ATCC strain (Satureja horvatii, Mentha pulegium and Rosmarinus officinalis) and other three in control of E. faecalis clinical isolates (Leptospermum petersonii, Thymus algeriensis, Thymus serpyllum). Thymol is a major component in three out of the six aforementioned most efficient EOs. The aim of our study was to investigate differences in efficacy of selected EOs that proved to possess great antimicrobial activity, towards the referent strain ATCC 29212 and clinical isolates of E. faecalis on, and to estimate which of their constituents might contribute to desired activity, as 'markers compunds'., Pregledom literature odabrano je 21 etarsko ulje (EU) koje pokazuje izuzetno značajan antimikrobni efekat prema kriterijumima koje smo zadali (MIC ≤ 200 μL / mL) na Enterococcus faecalis (ATCC 29212 referentni soj ili klinički izolat); Najbolji antimikrobni efekat sa MIC 0.4 μL / mL (približno 0.26 μg / mL) pokazalo je etarsko ulje Satureja horvatii L., dok su sa najslabijim antimikrobnim efektom bila ulja Rosmarinus officinalis L. i Achilea milefolium L. (MIC 160.0 μg / mL). Međugrupnom analizom MIC vrednosti, utvrđeno je da se MIC vrednosti, i u grupi u kojoj su testirana ulja na referentne sojeve i u grupi na kliničkim izolatima, razlikuju. MIC vrednosti ulja koja su delovala na referentni soj E. faecalis ATCC 29212, variraju od 0.26 to 156 μg / mL , dok je opseg MIC vrednosti koja pokazuju ulja efikasna na kliničke izolate E. faecalisa bio između 10 to 160 μg / mL. Dvanaest komponenti koje su zajedničke za etarska ulja testirana i na referentnom soju i na kliničkim izolatima, a čiji MIC ≤ 200 μg / mL , iskazana su u opadajućem nizu u odnosu na broj ulja u kojima su sadržana: trans-β-kariofilen (13) > mircen (8) > α-pinen (8) > linalool (7) > p-cymen (7) > borneol (7) > geraniol (6) > kamfen (6) > limonen (5) > 1,8-cineol (5) > γ-terpinen (5) > α-terpinen (4). Poređenjem komponenti utvrđeno je da su samo dve komponente, geraniol i 1,8-cineol, prisutne u procentualnoj zastupljenosti ≥ 10 % u više od jednog etarskog ulja (MIC opseg ulja 0.3-200 μg / mL ), a koja su pokazala efikasnost i na ATCC i na kliničke sojeve E. faecalis. Geraniol i 1,8-cineol su glavne komponente sadržaja ≥ 10 % u više ulja efikasnih i protiv ATCC soja i kliničkih izolata (MIC 0.3-200 μg / mL). U 11 ulja sa MIC ≤ 200 μg / mL na E. faecalis ATCC 29212, uočeno je 13 reprezentativnih komponenti, predstavljanih opadajućem nizu u odnosu na zastupljenost u uljima: eugenol 82.9 % > timol 63.7 % > heksadekanoanska kiselina 47.8 % > mentol 46.6 % > cis-b-ocimen 44.2 % > geranial 42.1 % > trans-β-kariofilen 40.8 % > citronelal 36.7 % > α-pinen 31.2 % > neral 30.5 % > α-eudesmol 22.4 % > citronelol 13.1 % > menton 11.3 %. U 10 etarskih ulja sa MIC ≤ 200 μg / mL efikasnim na klinički izolat, 7 je reprezentativnih i one su prikazane u opadajućem nizu u odnosu na njihovu zastupljenost u EU: feniletil alkohol 57.7 % > geranial 32.9 % > neral 22.2 % > p-cimen 20 % > karvakrol, 14 % > α-pinen 11.5 % > linalool 11.4 %. Od 21 EU iz ovog pregleda, šest su se izdvojila kao najefikasnija (MIC ≤ 30 μg / mL); 3 na referentni E. faecalis ATCC soj (Satureja horvatii, Mentha pulegium and Rosmarinus officinalis) i druga 3 na E faecalis klinički izolat (Leptospermum petersonii, Thymus algeriensis, Thymus serpyllum). Timol je bio procentualno najzastupljenija komponenta u 3 od 6 najefikasnijih EU. Cilj ovog istraživanja bio je da se utvrde razlike u efikasnosti etarskih ulja koja su odabrana zbog njihove jake antimikrobne aktivnosti na kliničke izolate E. faecalis kao i na referentni soj ATCC 29212, i da se izvrši procena njihovog hemijskog sastava vezano za komponente koje svojim učešćem doprinose antimikrobnoj aktivnosti i mogu poslužiti kao 'markeri efikasnosti'.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Analysis of chemical composition of the most efficient essential oils towards Enterococcus faecalis referent strain ATCC 29212 and clinical isolates, Analiza hemijskog sastava etarskih ulja snažne efikasnosti na referentni soj ATCC 29212 i kliničke izolate Enterococcus faecalis",
number = "36",
pages = "3-25",
doi = "10.5937/leksir1636003B"
}

Bogojević, J., Nikolić, M., Marković, T., Ćirić, A.,& Marković, D.. (2016). Analysis of chemical composition of the most efficient essential oils towards Enterococcus faecalis referent strain ATCC 29212 and clinical isolates. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(36), 3-25.
https://doi.org/10.5937/leksir1636003B

Bogojević J, Nikolić M, Marković T, Ćirić A, Marković D. Analysis of chemical composition of the most efficient essential oils towards Enterococcus faecalis referent strain ATCC 29212 and clinical isolates. in Lekovite sirovine. 2016;(36):3-25.
doi:10.5937/leksir1636003B .

Bogojević, Jelena, Nikolić, Miloš, Marković, Tatjana, Ćirić, Ana, Marković, Dejan, "Analysis of chemical composition of the most efficient essential oils towards Enterococcus faecalis referent strain ATCC 29212 and clinical isolates" in Lekovite sirovine, no. 36 (2016):3-25,
https://doi.org/10.5937/leksir1636003B . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Marković, Tatjana
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Marković, Dejan
AU  - Soković, Marina
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2065
AB  - The aim of this study was to investigate the chemical composition and antifungal activity of four essential oils from Apiaceae family, Pastinaca sativa, Foeniculum vulgare, Pimpinella anisum and Anethum graveolens, against Candida spp. reference strains and clinical isolates from human oral cavities. Chemical analysis of the essential oils (GCFID and GC/MS) showed that predominating chemical classes in the essential oil of A. graveolens was monoterpene hydrocarbons (49.4 %) and the major constituent of the oil was carvone (42.2 %): while in the oils of P. anisum, F. vulgare and P. sativa majority of compounds belong to phenylpropenes (80.9 %, 65.9 % and 49.4 %, respectively): and the major oil constituents were trans-anethole (65.1 % and 80.8 %) and myristicine (49.0 %): respectively. Minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations were determined using microdilution method. All the tested oils inhibited growth of Candida spp. The strongest antifungal activity was shown by A. graveolens essential oil (MIC 0.63-2.5 mg mL-1; MFC 1.25-5 mg mL-1). In addition to the common use of parsnip, fennel, anise and dill in traditional medicine, present study validates use of their essential oils in treatments intended for reduction and elimination of Candida spp. population in human patients with fungal infection.
AB  - Cilj ovog istraživanja bio je da se ispita hemijski sastav i antifungalna aktivnost četiri etarska ulja familije Apiaceae, Pastinaca sativa, Foeniculum vulgare, Pimpinella anisum i Anethum graveolens, na referentne sojeve i kliničke izolate Candida spp. iz usne duplje ljudi. Hemijska analiza ispitivanih etarskih ulja (GC-FID i GC/MS) je pokazala da u uljima dominiraju sledeće hemijske klase: u ulju A. graveolens - monoterpenski ugljovodonici (49,4%) i glavni sastojak je karvon (42,2 %): dok u uljima P. anisum F. vulgare P. sativa većina jedinjenja pripada fenilpropenima (80,9 %, 65,9 % i 49,4 %, respektivno): pri čemu su glavne komponente prva dva ulja trans-anetol (65,1 % i 80.8 %): a trećeg, miristicin (49.0 %). Minimalne inhibitorne (MIC) i minimalne fungicidne koncentracije ulja (MFC) su određene primenom mikrodilucione metode. Ispitivana tri ulja su inhibirala rast svih Candida spp.; najjaču antifungalnu aktivnost je pokazalo ulje A. graveolens (MIC 0.63-2.5 mg ml-1; MFC 1.25-5 mg ml-1). Pored česte primene paškanata, morača, anisa i mirođije u tradicionalnoj medicini, ova studija potvrđuje upotrebu njihovih etarskih ulja u tretmanima redukcije i eliminisanju populacije Candida spp. kod humanih pacijenata sa gljivičnim infekcijama.
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Susceptibility of oral Candida spp.: Reference strains and clinical isolates to selected essential oils of Apiaceae species
T1  - Osetljivost referentnih sojeva i oralnih kliničkih izolata Candida spp. - na odabrana etarska ulja familije Apiaceae
IS  - 35
SP  - 151
EP  - 162
DO  - 10.5937/leksir1535151N
ER  - 

@article{
author = "Nikolić, Miloš and Marković, Tatjana and Ćirić, Ana and Glamočlija, Jasmina and Marković, Dejan and Soković, Marina",
year = "2015",
abstract = "The aim of this study was to investigate the chemical composition and antifungal activity of four essential oils from Apiaceae family, Pastinaca sativa, Foeniculum vulgare, Pimpinella anisum and Anethum graveolens, against Candida spp. reference strains and clinical isolates from human oral cavities. Chemical analysis of the essential oils (GCFID and GC/MS) showed that predominating chemical classes in the essential oil of A. graveolens was monoterpene hydrocarbons (49.4 %) and the major constituent of the oil was carvone (42.2 %): while in the oils of P. anisum, F. vulgare and P. sativa majority of compounds belong to phenylpropenes (80.9 %, 65.9 % and 49.4 %, respectively): and the major oil constituents were trans-anethole (65.1 % and 80.8 %) and myristicine (49.0 %): respectively. Minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations were determined using microdilution method. All the tested oils inhibited growth of Candida spp. The strongest antifungal activity was shown by A. graveolens essential oil (MIC 0.63-2.5 mg mL-1; MFC 1.25-5 mg mL-1). In addition to the common use of parsnip, fennel, anise and dill in traditional medicine, present study validates use of their essential oils in treatments intended for reduction and elimination of Candida spp. population in human patients with fungal infection., Cilj ovog istraživanja bio je da se ispita hemijski sastav i antifungalna aktivnost četiri etarska ulja familije Apiaceae, Pastinaca sativa, Foeniculum vulgare, Pimpinella anisum i Anethum graveolens, na referentne sojeve i kliničke izolate Candida spp. iz usne duplje ljudi. Hemijska analiza ispitivanih etarskih ulja (GC-FID i GC/MS) je pokazala da u uljima dominiraju sledeće hemijske klase: u ulju A. graveolens - monoterpenski ugljovodonici (49,4%) i glavni sastojak je karvon (42,2 %): dok u uljima P. anisum F. vulgare P. sativa većina jedinjenja pripada fenilpropenima (80,9 %, 65,9 % i 49,4 %, respektivno): pri čemu su glavne komponente prva dva ulja trans-anetol (65,1 % i 80.8 %): a trećeg, miristicin (49.0 %). Minimalne inhibitorne (MIC) i minimalne fungicidne koncentracije ulja (MFC) su određene primenom mikrodilucione metode. Ispitivana tri ulja su inhibirala rast svih Candida spp.; najjaču antifungalnu aktivnost je pokazalo ulje A. graveolens (MIC 0.63-2.5 mg ml-1; MFC 1.25-5 mg ml-1). Pored česte primene paškanata, morača, anisa i mirođije u tradicionalnoj medicini, ova studija potvrđuje upotrebu njihovih etarskih ulja u tretmanima redukcije i eliminisanju populacije Candida spp. kod humanih pacijenata sa gljivičnim infekcijama.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Susceptibility of oral Candida spp.: Reference strains and clinical isolates to selected essential oils of Apiaceae species, Osetljivost referentnih sojeva i oralnih kliničkih izolata Candida spp. - na odabrana etarska ulja familije Apiaceae",
number = "35",
pages = "151-162",
doi = "10.5937/leksir1535151N"
}

Nikolić, M., Marković, T., Ćirić, A., Glamočlija, J., Marković, D.,& Soković, M.. (2015). Susceptibility of oral Candida spp.: Reference strains and clinical isolates to selected essential oils of Apiaceae species. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(35), 151-162.
https://doi.org/10.5937/leksir1535151N

Nikolić M, Marković T, Ćirić A, Glamočlija J, Marković D, Soković M. Susceptibility of oral Candida spp.: Reference strains and clinical isolates to selected essential oils of Apiaceae species. in Lekovite sirovine. 2015;(35):151-162.
doi:10.5937/leksir1535151N .

Nikolić, Miloš, Marković, Tatjana, Ćirić, Ana, Glamočlija, Jasmina, Marković, Dejan, Soković, Marina, "Susceptibility of oral Candida spp.: Reference strains and clinical isolates to selected essential oils of Apiaceae species" in Lekovite sirovine, no. 35 (2015):151-162,
https://doi.org/10.5937/leksir1535151N . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Glamočlija, Jasmina
AU  - Ferreira, Isabel C. F. R.
AU  - Calhelha, Ricardo C.
AU  - Fernandes, Angela
AU  - Marković, Tatjana
AU  - Marković, Dejan
AU  - Giweli, Abdulhamed
AU  - Soković, Marina
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1949
AB  - Aromatic plant species of genus Thymus are important medicinal plants, highly recommended due to a range of therapeutic properties of their essential oils, commonly known as thyme oil: antirheumatic, antiseptic, antispasmodic, antimicrobial, cardiac, carminative, diuretic and expectorant. The oil is also beneficial in boosting the immune system and helps to fight colds, flu, infectious diseases and chills. It is proved to be a urinary antiseptic, being very helpful for cystitis and urethritis. Scientific validation of traditional uses, and phytochemical and bioactivity evaluation of essential oils from Thymus serpyllum, Thymus algeriensis and Thymus vulgaris were performed. GC/MS analysis revealed thymol as a major component of T. algeriensis, T. vulgaris and T. serpyllum, with its contribution to the oil 56.0%, 48.9% and 38.5%, respectively. All three essential oils (EOs) exhibited a significant antimicrobial activity against all tested strains, the T. serpyllum oil being the most potent one (MIC 2.5-5 mu g/mL, MBC 5-10 mu g/mL; MIC 1-2 mu g/mL, MFC 2-4 mu g/mL). In addition, the T. serpyllum oil revealed the highest antioxidant activity in all conducted assays and it was the most effective one against all tested cell lines, presenting GI(50) values of 7.02-52.69 mu g/mL. Moreover, none of the EOs showed toxicity at tested concentrations (>400 mu g/mL) for porcine liver primary cell culture. In addition to their well-known traditional use in food and cosmetics, the great potential of tested Thymus essential oils for application in oral diseases and anticancer treatments, encourage further investigation.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops & Products
T1  - Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils
VL  - 52
SP  - 183
EP  - 190
DO  - 10.1016/j.indcrop.2013.10.006
ER  - 

@article{
author = "Nikolić, Miloš and Glamočlija, Jasmina and Ferreira, Isabel C. F. R. and Calhelha, Ricardo C. and Fernandes, Angela and Marković, Tatjana and Marković, Dejan and Giweli, Abdulhamed and Soković, Marina",
year = "2014",
abstract = "Aromatic plant species of genus Thymus are important medicinal plants, highly recommended due to a range of therapeutic properties of their essential oils, commonly known as thyme oil: antirheumatic, antiseptic, antispasmodic, antimicrobial, cardiac, carminative, diuretic and expectorant. The oil is also beneficial in boosting the immune system and helps to fight colds, flu, infectious diseases and chills. It is proved to be a urinary antiseptic, being very helpful for cystitis and urethritis. Scientific validation of traditional uses, and phytochemical and bioactivity evaluation of essential oils from Thymus serpyllum, Thymus algeriensis and Thymus vulgaris were performed. GC/MS analysis revealed thymol as a major component of T. algeriensis, T. vulgaris and T. serpyllum, with its contribution to the oil 56.0%, 48.9% and 38.5%, respectively. All three essential oils (EOs) exhibited a significant antimicrobial activity against all tested strains, the T. serpyllum oil being the most potent one (MIC 2.5-5 mu g/mL, MBC 5-10 mu g/mL; MIC 1-2 mu g/mL, MFC 2-4 mu g/mL). In addition, the T. serpyllum oil revealed the highest antioxidant activity in all conducted assays and it was the most effective one against all tested cell lines, presenting GI(50) values of 7.02-52.69 mu g/mL. Moreover, none of the EOs showed toxicity at tested concentrations (>400 mu g/mL) for porcine liver primary cell culture. In addition to their well-known traditional use in food and cosmetics, the great potential of tested Thymus essential oils for application in oral diseases and anticancer treatments, encourage further investigation.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops & Products",
title = "Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils",
volume = "52",
pages = "183-190",
doi = "10.1016/j.indcrop.2013.10.006"
}

Nikolić, M., Glamočlija, J., Ferreira, I. C. F. R., Calhelha, R. C., Fernandes, A., Marković, T., Marković, D., Giweli, A.,& Soković, M.. (2014). Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils. in Industrial Crops & Products
Elsevier, Amsterdam., 52, 183-190.
https://doi.org/10.1016/j.indcrop.2013.10.006

Nikolić M, Glamočlija J, Ferreira ICFR, Calhelha RC, Fernandes A, Marković T, Marković D, Giweli A, Soković M. Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils. in Industrial Crops & Products. 2014;52:183-190.
doi:10.1016/j.indcrop.2013.10.006 .

Nikolić, Miloš, Glamočlija, Jasmina, Ferreira, Isabel C. F. R., Calhelha, Ricardo C., Fernandes, Angela, Marković, Tatjana, Marković, Dejan, Giweli, Abdulhamed, Soković, Marina, "Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils" in Industrial Crops & Products, 52 (2014):183-190,
https://doi.org/10.1016/j.indcrop.2013.10.006 . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Jovanović, Katarina K.
AU  - Marković, Tatjana
AU  - Marković, Dejan
AU  - Gligorijević, Nevenka
AU  - Radulović, Siniša
AU  - Soković, Marina
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1882
AB  - The aim of this study was to assess the biological activity of essential oils from five Lamiaceae species, Mentha piperita, Mentha pulegium, Lavandula angustifolia, Satureja montana and Salvia lavandulifolia, for their chemical composition, antimicrobial and cytotoxic properties. Gas chromatography coupled with FID and MS of essential oils revealed menthol (47.5%), pulegone (68.7), linalool (40.3%), thymol (44.6%), and camphor (29.1%) to be major oils' components, respectively. Minimum inhibitory (MIC) and minimum bactericidal/fungicidal (MBC/MFC) concentrations were determined by microdilution method. Seven bacterial species, representing clinical specimens, including Streptococcus mutans, Streptococcus sanguis, Streptococcus salivarius, Streptococcus pyogenes, Pseudomonas aeruginosa, Lactobacillus acidophilus, and Enterecoccus feacalis, and fifty eight clinical oral Candida spp. isolates along with three reference strains were used in experiment. All essential oils (EOs) exhibited a significant antimicrobial activity against all tested microorganisms; the oil of S. montana proved to be the most potent one (MIC 30.0-630.0 mu g/mL, MBC 60.0-250.0 mu g/mL; MIC 0.9-1.0 mu g/mL; MFC 1.0-3.0 mu g/mL). In addition, the oil also revealed the highest cytotoxic activity against tested cell lines, presenting IC50 values from 40.13 to 65.51 mu g/mL, with mild selectivity towards HeLa cells observed in regard to the normal cell line (MRC-5). In addition to the herbs traditional use in food and pharmacy, results of this study proved the great potential of their essential oils for application in oral disease and anticancer treatments.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops & Products
T1  - Chemical composition, antimicrobial, and cytotoxic properties of five Lamiaceae essential oils
VL  - 61
SP  - 225
EP  - 232
DO  - 10.1016/j.indcrop.2014.07.011
ER  - 

@article{
author = "Nikolić, Miloš and Jovanović, Katarina K. and Marković, Tatjana and Marković, Dejan and Gligorijević, Nevenka and Radulović, Siniša and Soković, Marina",
year = "2014",
abstract = "The aim of this study was to assess the biological activity of essential oils from five Lamiaceae species, Mentha piperita, Mentha pulegium, Lavandula angustifolia, Satureja montana and Salvia lavandulifolia, for their chemical composition, antimicrobial and cytotoxic properties. Gas chromatography coupled with FID and MS of essential oils revealed menthol (47.5%), pulegone (68.7), linalool (40.3%), thymol (44.6%), and camphor (29.1%) to be major oils' components, respectively. Minimum inhibitory (MIC) and minimum bactericidal/fungicidal (MBC/MFC) concentrations were determined by microdilution method. Seven bacterial species, representing clinical specimens, including Streptococcus mutans, Streptococcus sanguis, Streptococcus salivarius, Streptococcus pyogenes, Pseudomonas aeruginosa, Lactobacillus acidophilus, and Enterecoccus feacalis, and fifty eight clinical oral Candida spp. isolates along with three reference strains were used in experiment. All essential oils (EOs) exhibited a significant antimicrobial activity against all tested microorganisms; the oil of S. montana proved to be the most potent one (MIC 30.0-630.0 mu g/mL, MBC 60.0-250.0 mu g/mL; MIC 0.9-1.0 mu g/mL; MFC 1.0-3.0 mu g/mL). In addition, the oil also revealed the highest cytotoxic activity against tested cell lines, presenting IC50 values from 40.13 to 65.51 mu g/mL, with mild selectivity towards HeLa cells observed in regard to the normal cell line (MRC-5). In addition to the herbs traditional use in food and pharmacy, results of this study proved the great potential of their essential oils for application in oral disease and anticancer treatments.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops & Products",
title = "Chemical composition, antimicrobial, and cytotoxic properties of five Lamiaceae essential oils",
volume = "61",
pages = "225-232",
doi = "10.1016/j.indcrop.2014.07.011"
}

Nikolić, M., Jovanović, K. K., Marković, T., Marković, D., Gligorijević, N., Radulović, S.,& Soković, M.. (2014). Chemical composition, antimicrobial, and cytotoxic properties of five Lamiaceae essential oils. in Industrial Crops & Products
Elsevier Science Bv, Amsterdam., 61, 225-232.
https://doi.org/10.1016/j.indcrop.2014.07.011

Nikolić M, Jovanović KK, Marković T, Marković D, Gligorijević N, Radulović S, Soković M. Chemical composition, antimicrobial, and cytotoxic properties of five Lamiaceae essential oils. in Industrial Crops & Products. 2014;61:225-232.
doi:10.1016/j.indcrop.2014.07.011 .

Nikolić, Miloš, Jovanović, Katarina K., Marković, Tatjana, Marković, Dejan, Gligorijević, Nevenka, Radulović, Siniša, Soković, Marina, "Chemical composition, antimicrobial, and cytotoxic properties of five Lamiaceae essential oils" in Industrial Crops & Products, 61 (2014):225-232,
https://doi.org/10.1016/j.indcrop.2014.07.011 . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Marković, Tatjana
AU  - Mojović, Miloš
AU  - Pejin, Boris
AU  - Savić, Aleksandar
AU  - Perić, Tamara
AU  - Marković, Dejan
AU  - Stević, Tatjana
AU  - Soković, Marina
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1829
AB  - The aim of this study was to examine chemical composition and biological activity of Gaultheria procumbens L essential oil (EO) against food spoilage and oral microorganisms. The components of EO were identified by GC-MS. Antimicrobial activity was determined against food spoilage (five bacteria and six fungal species) and oral microorganisms (eight bacteria and thirty two fungal species) by microdilution and microplate biofilm assay, antioxidant activity was tested using the persistent free radical 2,2-diphenyl-1-picryl hydrazyl (DPPH), while antiradical activity was examined by fluorescence spectroscopy and electron paramagnetic resonance spectroscopy (EPR). GC-MS analysis showed that methyl salicylate (96.90%) was the main component of the oil. Essential oil inhibited the growth of all microorganisms tested, i.e. food and oral bacteria and fungi, respectively (MIC 0.18-3.00 mg/ml and MBC 1.25-4.00 mg/ml; MIC 0.73-5.00 mg/ml and MFC 2.92-26.67 mg/ml); The oil effectively inhibited the biofilm formation of oral Streptococcus mutans and Candida albicans as well (MIC 25.00 MBC 50.00 mg/ml; MIC 12.50, MFC 50.00 mg/ml). In addition, oil exhibited a dose-dependent DPPH-radical-scavenging activity with IC50 value of 30.61 mg/ml. The specific fluorescence probe 2-[6-(4'-amino)phenoxy-3H-xanten-3-on-9-yl] benzoic acid (APP) and the the spin trap 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO), capable for simultaneous detection of different free radical specie were used in antiradical activity of the oil measurements. Oil showed a moderate antiradical activity, reducing quantity of produced hydroxyl radicals to about 20% of initial value. This study succeeds in creating directly comparable and quantitative data for the oil unsufficiently examined so far.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops & Products
T1  - Chemical composition and biological activity of Gaultheria procumbens L. essential oil
VL  - 49
SP  - 561
EP  - 567
DO  - 10.1016/j.indcrop.2013.06.002
ER  - 

@article{
author = "Nikolić, Miloš and Marković, Tatjana and Mojović, Miloš and Pejin, Boris and Savić, Aleksandar and Perić, Tamara and Marković, Dejan and Stević, Tatjana and Soković, Marina",
year = "2013",
abstract = "The aim of this study was to examine chemical composition and biological activity of Gaultheria procumbens L essential oil (EO) against food spoilage and oral microorganisms. The components of EO were identified by GC-MS. Antimicrobial activity was determined against food spoilage (five bacteria and six fungal species) and oral microorganisms (eight bacteria and thirty two fungal species) by microdilution and microplate biofilm assay, antioxidant activity was tested using the persistent free radical 2,2-diphenyl-1-picryl hydrazyl (DPPH), while antiradical activity was examined by fluorescence spectroscopy and electron paramagnetic resonance spectroscopy (EPR). GC-MS analysis showed that methyl salicylate (96.90%) was the main component of the oil. Essential oil inhibited the growth of all microorganisms tested, i.e. food and oral bacteria and fungi, respectively (MIC 0.18-3.00 mg/ml and MBC 1.25-4.00 mg/ml; MIC 0.73-5.00 mg/ml and MFC 2.92-26.67 mg/ml); The oil effectively inhibited the biofilm formation of oral Streptococcus mutans and Candida albicans as well (MIC 25.00 MBC 50.00 mg/ml; MIC 12.50, MFC 50.00 mg/ml). In addition, oil exhibited a dose-dependent DPPH-radical-scavenging activity with IC50 value of 30.61 mg/ml. The specific fluorescence probe 2-[6-(4'-amino)phenoxy-3H-xanten-3-on-9-yl] benzoic acid (APP) and the the spin trap 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO), capable for simultaneous detection of different free radical specie were used in antiradical activity of the oil measurements. Oil showed a moderate antiradical activity, reducing quantity of produced hydroxyl radicals to about 20% of initial value. This study succeeds in creating directly comparable and quantitative data for the oil unsufficiently examined so far.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops & Products",
title = "Chemical composition and biological activity of Gaultheria procumbens L. essential oil",
volume = "49",
pages = "561-567",
doi = "10.1016/j.indcrop.2013.06.002"
}

Nikolić, M., Marković, T., Mojović, M., Pejin, B., Savić, A., Perić, T., Marković, D., Stević, T.,& Soković, M.. (2013). Chemical composition and biological activity of Gaultheria procumbens L. essential oil. in Industrial Crops & Products
Elsevier, Amsterdam., 49, 561-567.
https://doi.org/10.1016/j.indcrop.2013.06.002

Nikolić M, Marković T, Mojović M, Pejin B, Savić A, Perić T, Marković D, Stević T, Soković M. Chemical composition and biological activity of Gaultheria procumbens L. essential oil. in Industrial Crops & Products. 2013;49:561-567.
doi:10.1016/j.indcrop.2013.06.002 .

Nikolić, Miloš, Marković, Tatjana, Mojović, Miloš, Pejin, Boris, Savić, Aleksandar, Perić, Tamara, Marković, Dejan, Stević, Tatjana, Soković, Marina, "Chemical composition and biological activity of Gaultheria procumbens L. essential oil" in Industrial Crops & Products, 49 (2013):561-567,
https://doi.org/10.1016/j.indcrop.2013.06.002 . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Marković, Tatjana
AU  - Marković, Dejan
AU  - Perić, Tamara
AU  - Soković, Marina
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1802
AB  - The aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils of peppermint Mentha piperita L. GC / MS techniques confirmed the presence of the 48 components of which menthol (35.57 %) and menthone (22.50 %) were the most abundant. Antimicrobial activity, ie the minimum inhibitory and minimum bactericidal / fungicidal (MIC, MBC/MFC) concentrations were obtained using the mikordilution method against pathogenic microorganism isolated from the oral cavity (8 bacteria and 58 Candida sp.) and referent strains. The essential oil of peppermint showed good antimicrobial activity with MIC from 0.63 to 5 mg / ml and 0.13 to 0.5 for bacteria and fungi and MBC 1.25 to 10 mg / ml , ie. MFC 0.5-1 mg / ml. Compared to Hexoral®, peppermint essential oil has a very strong antimicrobial potential. The results of these tests give a new value, which will enrich the knowledge of peppermint oil od presented chemical composition, opens the door for further research towards its use in dentistry and as well in prevention and treatment of various diseases caused by tested pathogens.
AB  - Cilj ovog rada je bio da se ispita hemijski sastav i antimikrobna aktivnost etarskog ulja pitome nane Mentha piperita L. GC/MS analizom utvrđeno je prisustvo ukupno 48 komponenata od koji su mentol (35.57 %) i menton (22.50 %) bile najzastupljenije. Antimikobna aktivnost tj minimalne inhibitorne i minimalne baktericidne/fungicidne (MIC, MBC/MFC) koncentracije dobijene su mikordilucionom metodom na patogene mikoorganizme izolovane iz usne duplje (8 bakterija i 58 Candida spp.) i referentne sojeve. Etarsko ulje pitome nane je pokazalo dobru antimikrobnu aktivnost, sa MIC 0.63-5 mg/ml i 0.13-0.5 za bakterije i gljive i MBC 1.25-10.0 mg/ml tj. MFC 0.5-1.0 mg/ml. U poređenju sa Hexoralom®, etarsko ulje pitome nane je posedovalo veoma jak antimikrobni potencijal. Rezultati ovih ispitivanja daju novu vrednost, koja će obogatiti saznanja o etarskom ulju pitome nane poznatog hemijskog sastava i otvarati vrata za dalja istraživanja ka korišćenju njenog etarskog ulja kao prirodnog terapeutskog preparata u stomatologiji, kako u preventivi tako i u tretmanima raznih oboljenja biljaka, životinja i ljudi.
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Chemical composition and antimicrobial activity of peppermint essential oil (Mentha piperita L.)
T1  - Hemijski sastav i antimikrobna aktivnost etarskog ulja pitome nane (Mentha piperita L.)
IS  - 33
SP  - 63
EP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_smile_1802
ER  - 

@article{
author = "Nikolić, Miloš and Glamočlija, Jasmina and Ćirić, Ana and Marković, Tatjana and Marković, Dejan and Perić, Tamara and Soković, Marina",
year = "2013",
abstract = "The aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils of peppermint Mentha piperita L. GC / MS techniques confirmed the presence of the 48 components of which menthol (35.57 %) and menthone (22.50 %) were the most abundant. Antimicrobial activity, ie the minimum inhibitory and minimum bactericidal / fungicidal (MIC, MBC/MFC) concentrations were obtained using the mikordilution method against pathogenic microorganism isolated from the oral cavity (8 bacteria and 58 Candida sp.) and referent strains. The essential oil of peppermint showed good antimicrobial activity with MIC from 0.63 to 5 mg / ml and 0.13 to 0.5 for bacteria and fungi and MBC 1.25 to 10 mg / ml , ie. MFC 0.5-1 mg / ml. Compared to Hexoral®, peppermint essential oil has a very strong antimicrobial potential. The results of these tests give a new value, which will enrich the knowledge of peppermint oil od presented chemical composition, opens the door for further research towards its use in dentistry and as well in prevention and treatment of various diseases caused by tested pathogens., Cilj ovog rada je bio da se ispita hemijski sastav i antimikrobna aktivnost etarskog ulja pitome nane Mentha piperita L. GC/MS analizom utvrđeno je prisustvo ukupno 48 komponenata od koji su mentol (35.57 %) i menton (22.50 %) bile najzastupljenije. Antimikobna aktivnost tj minimalne inhibitorne i minimalne baktericidne/fungicidne (MIC, MBC/MFC) koncentracije dobijene su mikordilucionom metodom na patogene mikoorganizme izolovane iz usne duplje (8 bakterija i 58 Candida spp.) i referentne sojeve. Etarsko ulje pitome nane je pokazalo dobru antimikrobnu aktivnost, sa MIC 0.63-5 mg/ml i 0.13-0.5 za bakterije i gljive i MBC 1.25-10.0 mg/ml tj. MFC 0.5-1.0 mg/ml. U poređenju sa Hexoralom®, etarsko ulje pitome nane je posedovalo veoma jak antimikrobni potencijal. Rezultati ovih ispitivanja daju novu vrednost, koja će obogatiti saznanja o etarskom ulju pitome nane poznatog hemijskog sastava i otvarati vrata za dalja istraživanja ka korišćenju njenog etarskog ulja kao prirodnog terapeutskog preparata u stomatologiji, kako u preventivi tako i u tretmanima raznih oboljenja biljaka, životinja i ljudi.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Chemical composition and antimicrobial activity of peppermint essential oil (Mentha piperita L.), Hemijski sastav i antimikrobna aktivnost etarskog ulja pitome nane (Mentha piperita L.)",
number = "33",
pages = "63-72",
url = "https://hdl.handle.net/21.15107/rcub_smile_1802"
}

Nikolić, M., Glamočlija, J., Ćirić, A., Marković, T., Marković, D., Perić, T.,& Soković, M.. (2013). Chemical composition and antimicrobial activity of peppermint essential oil (Mentha piperita L.). in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(33), 63-72.
https://hdl.handle.net/21.15107/rcub_smile_1802

Nikolić M, Glamočlija J, Ćirić A, Marković T, Marković D, Perić T, Soković M. Chemical composition and antimicrobial activity of peppermint essential oil (Mentha piperita L.). in Lekovite sirovine. 2013;(33):63-72.
https://hdl.handle.net/21.15107/rcub_smile_1802 .

Nikolić, Miloš, Glamočlija, Jasmina, Ćirić, Ana, Marković, Tatjana, Marković, Dejan, Perić, Tamara, Soković, Marina, "Chemical composition and antimicrobial activity of peppermint essential oil (Mentha piperita L.)" in Lekovite sirovine, no. 33 (2013):63-72,
https://hdl.handle.net/21.15107/rcub_smile_1802 .