TY  - JOUR
AU  - Stanković, Marija
AU  - Pantić, Igor
AU  - de Luka, Silvio R.
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - Milutinović-Smiljanić, Sanja
AU  - Pantić, Senka
AU  - Trbovich, Alexander M.
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2129
AB  - The aim of the study was to examine alteration and possible application of fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely inflamed tissue. Acute inflammation was induced by injection of turpentine oil into the right and left hind limb muscles of mice, whereas control animals received intramuscular saline injection. After 12h, animals were anesthetised and treated muscles collected. The tissue was stained by hematoxylin and eosin, digital micrographs produced, enabling determination of fractal dimension of the cells, angular second moment and correlation of studied tissue. Histopathological analysis showed presence of inflammatory infiltrate and tissue damage in inflammatory group, whereas tissue structure in control group was preserved, devoid of inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of treated tissue in inflammatory group decreased in comparison to the control group. In this study, we were first to observe and report that fractal dimension of the cells, angular second moment, and correlation were reduced in acutely inflamed tissue, indicating loss of overall complexity of the cells in the tissue, the tissue uniformity and structure regularity. Fractal dimension, angular second moment and correlation could be useful methods for quantification of structural changes in acute inflammation. Lay Description The aim of this study was to examine alteration, and possible application of mathematical parameters fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely-inflamed tissue. An acute inflammation was induced by injection of turpentine oil into mice muscles, whereas control group received intramuscular injection of saline. After 12 h animals were anesthetized, and treated muscles were collected. The tissue was stained, and photos of the tissue were made. Mathematical parameters, namely fractal dimension, angular second moment, and correlation of the tissue photo, were determined by computer program. Standard histopathological analysis showed that inflammatory infiltrate and tissue damage were present in inflammatory group, whereas tissue structure in control group was preserved, and without inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of the treated tissue in inflammatory group decreased, when compared to control group. In this study we reported, for the first time, that fractal dimension of the cells, angular second moment, and correlation had decreased in acutely-inflamed tissue, indicating loss of overall complexity of cells in tissue, tissue uniformity, and structure regularity. Fractal dimension, angular second moment, and correlation could be useful methods for quantification of structural changes in acute inflammation.
PB  - Wiley, Hoboken
T2  - Journal of Microscopy
T1  - Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation
VL  - 261
IS  - 3
SP  - 277
EP  - 284
DO  - 10.1111/jmi.12330
ER  - 

@article{
author = "Stanković, Marija and Pantić, Igor and de Luka, Silvio R. and Puškaš, Nela and Zaletel, Ivan and Milutinović-Smiljanić, Sanja and Pantić, Senka and Trbovich, Alexander M.",
year = "2016",
abstract = "The aim of the study was to examine alteration and possible application of fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely inflamed tissue. Acute inflammation was induced by injection of turpentine oil into the right and left hind limb muscles of mice, whereas control animals received intramuscular saline injection. After 12h, animals were anesthetised and treated muscles collected. The tissue was stained by hematoxylin and eosin, digital micrographs produced, enabling determination of fractal dimension of the cells, angular second moment and correlation of studied tissue. Histopathological analysis showed presence of inflammatory infiltrate and tissue damage in inflammatory group, whereas tissue structure in control group was preserved, devoid of inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of treated tissue in inflammatory group decreased in comparison to the control group. In this study, we were first to observe and report that fractal dimension of the cells, angular second moment, and correlation were reduced in acutely inflamed tissue, indicating loss of overall complexity of the cells in the tissue, the tissue uniformity and structure regularity. Fractal dimension, angular second moment and correlation could be useful methods for quantification of structural changes in acute inflammation. Lay Description The aim of this study was to examine alteration, and possible application of mathematical parameters fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely-inflamed tissue. An acute inflammation was induced by injection of turpentine oil into mice muscles, whereas control group received intramuscular injection of saline. After 12 h animals were anesthetized, and treated muscles were collected. The tissue was stained, and photos of the tissue were made. Mathematical parameters, namely fractal dimension, angular second moment, and correlation of the tissue photo, were determined by computer program. Standard histopathological analysis showed that inflammatory infiltrate and tissue damage were present in inflammatory group, whereas tissue structure in control group was preserved, and without inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of the treated tissue in inflammatory group decreased, when compared to control group. In this study we reported, for the first time, that fractal dimension of the cells, angular second moment, and correlation had decreased in acutely-inflamed tissue, indicating loss of overall complexity of cells in tissue, tissue uniformity, and structure regularity. Fractal dimension, angular second moment, and correlation could be useful methods for quantification of structural changes in acute inflammation.",
publisher = "Wiley, Hoboken",
journal = "Journal of Microscopy",
title = "Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation",
volume = "261",
number = "3",
pages = "277-284",
doi = "10.1111/jmi.12330"
}

Stanković, M., Pantić, I., de Luka, S. R., Puškaš, N., Zaletel, I., Milutinović-Smiljanić, S., Pantić, S.,& Trbovich, A. M.. (2016). Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation. in Journal of Microscopy
Wiley, Hoboken., 261(3), 277-284.
https://doi.org/10.1111/jmi.12330

Stanković M, Pantić I, de Luka SR, Puškaš N, Zaletel I, Milutinović-Smiljanić S, Pantić S, Trbovich AM. Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation. in Journal of Microscopy. 2016;261(3):277-284.
doi:10.1111/jmi.12330 .

Stanković, Marija, Pantić, Igor, de Luka, Silvio R., Puškaš, Nela, Zaletel, Ivan, Milutinović-Smiljanić, Sanja, Pantić, Senka, Trbovich, Alexander M., "Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation" in Journal of Microscopy, 261, no. 3 (2016):277-284,
https://doi.org/10.1111/jmi.12330 . .

TY  - CONF
AU  - Stanković, Marija
AU  - Pantić, I
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - de Luka, Silvio R.
AU  - Milutinović-Smiljanić, Sanja
AU  - Trbovich, Alexander M.
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2006
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy
T1  - The role of IL-33/ST2 axis in enhancing inflammation, tissue damage and structural changes in acute inflammation
VL  - 70
SP  - 188
EP  - 188
UR  - https://hdl.handle.net/21.15107/rcub_smile_2006
ER  - 

@conference{
author = "Stanković, Marija and Pantić, I and Puškaš, Nela and Zaletel, Ivan and de Luka, Silvio R. and Milutinović-Smiljanić, Sanja and Trbovich, Alexander M.",
year = "2015",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "The role of IL-33/ST2 axis in enhancing inflammation, tissue damage and structural changes in acute inflammation",
volume = "70",
pages = "188-188",
url = "https://hdl.handle.net/21.15107/rcub_smile_2006"
}

Stanković, M., Pantić, I., Puškaš, N., Zaletel, I., de Luka, S. R., Milutinović-Smiljanić, S.,& Trbovich, A. M.. (2015). The role of IL-33/ST2 axis in enhancing inflammation, tissue damage and structural changes in acute inflammation. in Allergy
Wiley-Blackwell, Hoboken., 70, 188-188.
https://hdl.handle.net/21.15107/rcub_smile_2006

Stanković M, Pantić I, Puškaš N, Zaletel I, de Luka SR, Milutinović-Smiljanić S, Trbovich AM. The role of IL-33/ST2 axis in enhancing inflammation, tissue damage and structural changes in acute inflammation. in Allergy. 2015;70:188-188.
https://hdl.handle.net/21.15107/rcub_smile_2006 .

Stanković, Marija, Pantić, I, Puškaš, Nela, Zaletel, Ivan, de Luka, Silvio R., Milutinović-Smiljanić, Sanja, Trbovich, Alexander M., "The role of IL-33/ST2 axis in enhancing inflammation, tissue damage and structural changes in acute inflammation" in Allergy, 70 (2015):188-188,
https://hdl.handle.net/21.15107/rcub_smile_2006 .

TY  - JOUR
AU  - Stanković, Marija S.
AU  - Turuntas, Vladimir
AU  - de Luka, Silvio R.
AU  - Janković, Saša
AU  - Stefanović, Srđan
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - Milutinović-Smiljanić, Sanja
AU  - Trbovich, Alexander M.
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2003
AB  - Aim: The aim of this study was to examine the role of the IL-33/ST2 pathway in pathogenesis of acute inflammation by investigating its possible role in alteration of iron and hematological parameters in experimental model of acute inflammation. Material and methods: Wild-type and ST2 knockout BALB/c mice were divided into four groups: wild-type control group, ST2-/- control group, wild-type inflammatory group, and ST2-/- inflammatory group. Acute inflammation was induced by intramuscular injection of turpentine oil, while control groups were injected with saline. After 12 h animals were anesthetized, and the treated tissue, blood and spleen were collected. Iron concentration in the treated tissue, hemoglobin blood concentration, mean corpuscular hemoglobin (MCH), hematocrit, erythrocyte, neutrophil and lymphocyte blood count, and erythrocytes percentage in spleen were determined. Results: Iron concentration in the treated tissue was significantly higher in wild-type inflammatory group (WT-I) when compared to both, the wild-type control group (WT-C) and ST2-/- inflammatory group (KO-I). There was no significant difference in iron concentration between ST2-/- control group (KO-C) and the KO-I. MCH had significantly decreased in WT-I when compared to WT-C, while there was no significant difference between KO-C and KO-I. Hemoglobin blood concentration significantly increased in KO-I in comparison to KO-C, while it did not significantly differ between WT-I and KO-I. Erythrocyte count and hematocrit had significantly increased, while the percentage of erythrocytes in spleen decreased in both inflammatory groups when compared to their controls. Neutrophil count significantly decreased in WT-I, when compared to WT-C. Lymphocyte count decreased in both inflammatory groups when compared to their controls. Conclusion: Results of this study indicate that the IL-33/ST2 axis could have a role in the alteration of iron in acute inflammation, namely in an increase of iron concentration at the site of acute inflammation and a decrease of blood mean corpuscular hemoglobin.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Experimental & Molecular Pathology
T1  - Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation
VL  - 99
IS  - 3
SP  - 687
EP  - 692
DO  - 10.1016/j.yexmp.2015.11.016
ER  - 

@article{
author = "Stanković, Marija S. and Turuntas, Vladimir and de Luka, Silvio R. and Janković, Saša and Stefanović, Srđan and Puškaš, Nela and Zaletel, Ivan and Milutinović-Smiljanić, Sanja and Trbovich, Alexander M.",
year = "2015",
abstract = "Aim: The aim of this study was to examine the role of the IL-33/ST2 pathway in pathogenesis of acute inflammation by investigating its possible role in alteration of iron and hematological parameters in experimental model of acute inflammation. Material and methods: Wild-type and ST2 knockout BALB/c mice were divided into four groups: wild-type control group, ST2-/- control group, wild-type inflammatory group, and ST2-/- inflammatory group. Acute inflammation was induced by intramuscular injection of turpentine oil, while control groups were injected with saline. After 12 h animals were anesthetized, and the treated tissue, blood and spleen were collected. Iron concentration in the treated tissue, hemoglobin blood concentration, mean corpuscular hemoglobin (MCH), hematocrit, erythrocyte, neutrophil and lymphocyte blood count, and erythrocytes percentage in spleen were determined. Results: Iron concentration in the treated tissue was significantly higher in wild-type inflammatory group (WT-I) when compared to both, the wild-type control group (WT-C) and ST2-/- inflammatory group (KO-I). There was no significant difference in iron concentration between ST2-/- control group (KO-C) and the KO-I. MCH had significantly decreased in WT-I when compared to WT-C, while there was no significant difference between KO-C and KO-I. Hemoglobin blood concentration significantly increased in KO-I in comparison to KO-C, while it did not significantly differ between WT-I and KO-I. Erythrocyte count and hematocrit had significantly increased, while the percentage of erythrocytes in spleen decreased in both inflammatory groups when compared to their controls. Neutrophil count significantly decreased in WT-I, when compared to WT-C. Lymphocyte count decreased in both inflammatory groups when compared to their controls. Conclusion: Results of this study indicate that the IL-33/ST2 axis could have a role in the alteration of iron in acute inflammation, namely in an increase of iron concentration at the site of acute inflammation and a decrease of blood mean corpuscular hemoglobin.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Experimental & Molecular Pathology",
title = "Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation",
volume = "99",
number = "3",
pages = "687-692",
doi = "10.1016/j.yexmp.2015.11.016"
}

Stanković, M. S., Turuntas, V., de Luka, S. R., Janković, S., Stefanović, S., Puškaš, N., Zaletel, I., Milutinović-Smiljanić, S.,& Trbovich, A. M.. (2015). Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation. in Experimental & Molecular Pathology
Academic Press Inc Elsevier Science, San Diego., 99(3), 687-692.
https://doi.org/10.1016/j.yexmp.2015.11.016

Stanković MS, Turuntas V, de Luka SR, Janković S, Stefanović S, Puškaš N, Zaletel I, Milutinović-Smiljanić S, Trbovich AM. Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation. in Experimental & Molecular Pathology. 2015;99(3):687-692.
doi:10.1016/j.yexmp.2015.11.016 .

Stanković, Marija S., Turuntas, Vladimir, de Luka, Silvio R., Janković, Saša, Stefanović, Srđan, Puškaš, Nela, Zaletel, Ivan, Milutinović-Smiljanić, Sanja, Trbovich, Alexander M., "Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation" in Experimental & Molecular Pathology, 99, no. 3 (2015):687-692,
https://doi.org/10.1016/j.yexmp.2015.11.016 . .