TY  - JOUR
AU  - Stojić, Vanja
AU  - Glišić, Branislav
AU  - Đukić, Ljiljana
AU  - Prokić, Bogomir
AU  - Janović, Aleksa
AU  - Stamenković, Zorana
AU  - Milutinović-Smiljanić, Sanja
AU  - Danilović, Vesna
AU  - Brković, Božidar
AU  - Roganović, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2512
AB  - Objective: We aimed to investigate alteration in cellular signaling mediated by vascular endothelial growth factor (VEGF) and parameters of oxidative stress/nitric oxide generation, superoxide dismutase (SOD) and neuronal nitric oxide synthase (nNOS), underlying altered functional mechanical loading of TMJ (temporomandibular joint) during lateral mandibular deviation. Design: Thirty-eight 5-week-old male Wistar rats were divided into experimental group, which received acrylic resin appliance that shifted mandible to the left during closure, and control group. Computed tomography and histomorphometry were used for condyle analyses, while samples of condyle, synovial membrane and m. masseter were analyzed with enzyme-linked immunosorbent assay and spectrophotometry to determine VEGF and nNOS protein concentrations, and SOD activity. Results: Experimental group of rats developed smaller and asymmetrical mandibles. Less of new bone and cartilage formation and larger bone marrow cavities area were found in the experimental group. Higher VEGF expression in condyle and m. masseter as well as higher nNOS expression in m. masseter and synovial membrane were found in the experimental compared to the control group. Alteration of SOD activity was found in m. masseter and synovial membrane in the experimental group. Conclusions: Lateral mandibular deviation induces mandibular and condylar morphological changes as well as significant cellular signaling alterations in condyle, synovial membrane and masticatory muscle. Cellular VEGF protein overexpression and oxidative stress/nitric oxide disbalance could be the mechanisms underlying unbalanced functional TMJ loading due to mandibular deviation.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Archives of Oral Biology
T1  - Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle
VL  - 110
DO  - 10.1016/j.archoralbio.2019.104599
ER  - 

@article{
author = "Stojić, Vanja and Glišić, Branislav and Đukić, Ljiljana and Prokić, Bogomir and Janović, Aleksa and Stamenković, Zorana and Milutinović-Smiljanić, Sanja and Danilović, Vesna and Brković, Božidar and Roganović, Jelena",
year = "2020",
abstract = "Objective: We aimed to investigate alteration in cellular signaling mediated by vascular endothelial growth factor (VEGF) and parameters of oxidative stress/nitric oxide generation, superoxide dismutase (SOD) and neuronal nitric oxide synthase (nNOS), underlying altered functional mechanical loading of TMJ (temporomandibular joint) during lateral mandibular deviation. Design: Thirty-eight 5-week-old male Wistar rats were divided into experimental group, which received acrylic resin appliance that shifted mandible to the left during closure, and control group. Computed tomography and histomorphometry were used for condyle analyses, while samples of condyle, synovial membrane and m. masseter were analyzed with enzyme-linked immunosorbent assay and spectrophotometry to determine VEGF and nNOS protein concentrations, and SOD activity. Results: Experimental group of rats developed smaller and asymmetrical mandibles. Less of new bone and cartilage formation and larger bone marrow cavities area were found in the experimental group. Higher VEGF expression in condyle and m. masseter as well as higher nNOS expression in m. masseter and synovial membrane were found in the experimental compared to the control group. Alteration of SOD activity was found in m. masseter and synovial membrane in the experimental group. Conclusions: Lateral mandibular deviation induces mandibular and condylar morphological changes as well as significant cellular signaling alterations in condyle, synovial membrane and masticatory muscle. Cellular VEGF protein overexpression and oxidative stress/nitric oxide disbalance could be the mechanisms underlying unbalanced functional TMJ loading due to mandibular deviation.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Archives of Oral Biology",
title = "Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle",
volume = "110",
doi = "10.1016/j.archoralbio.2019.104599"
}

Stojić, V., Glišić, B., Đukić, L., Prokić, B., Janović, A., Stamenković, Z., Milutinović-Smiljanić, S., Danilović, V., Brković, B.,& Roganović, J.. (2020). Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle. in Archives of Oral Biology
Pergamon-Elsevier Science Ltd, Oxford., 110.
https://doi.org/10.1016/j.archoralbio.2019.104599

Stojić V, Glišić B, Đukić L, Prokić B, Janović A, Stamenković Z, Milutinović-Smiljanić S, Danilović V, Brković B, Roganović J. Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle. in Archives of Oral Biology. 2020;110.
doi:10.1016/j.archoralbio.2019.104599 .

Stojić, Vanja, Glišić, Branislav, Đukić, Ljiljana, Prokić, Bogomir, Janović, Aleksa, Stamenković, Zorana, Milutinović-Smiljanić, Sanja, Danilović, Vesna, Brković, Božidar, Roganović, Jelena, "Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle" in Archives of Oral Biology, 110 (2020),
https://doi.org/10.1016/j.archoralbio.2019.104599 . .

TY  - JOUR
AU  - Dželetović, Bojan
AU  - Aleksić, Nikola
AU  - Radak, Đorđe
AU  - Stratimirović, Đorđe
AU  - Đukić, Ljiljana
AU  - Stojić, Dragica
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2499
AB  - Introduction: Age-related changes of dental pulp tissue and atherosclerosis of carotid arteries as its feeding arteries could influence the functionality of pulpal circulation. The objective of our study was to evaluate the effect of aging (physiological process) and carotid bifurcation atherosclerosis (pathologic process) on the pulpal microcirculatory system using multifractal analysis of the laser Doppler flowmetry signal. Methods: Three groups of 10 subjects were enrolled in the study: the young group (healthy subjects, 20-25 years), the middle-aged group (healthy subjects, 50-60 years), and the clinical group (subjects with carotid bifurcation atherosclerosis, 50-60 years). Pulpal blood flow (PBF) signals recorded by laser Doppler flowmetry were assessed by multifractal analysis that estimates Holder exponents of the signal. PBF levels, the average mean values, and the range of Holder exponents were obtained. Results: PBF levels were significantly higher in the young group compared with the middle-aged and clinical groups, and the difference between the middle-aged and clinical groups was not statistically significant. The range of the Holder exponents was narrower in the middle-aged and clinical groups than in the young group and narrower in the clinical group than in the middle-aged group. The average mean value of Holder exponents was significantly higher in the young group than in the middle-aged and clinical groups, whereas there was no significant difference between the middle-aged and clinical groups. Conclusions: Our study investigating the multifractality of the PBF signal showed that the aging process and carotid atherosclerosis could affect the complex structure of PBF oscillations and contribute to a better understanding of pulpal hemodynamics.
PB  - Elsevier Science Inc, New York
T2  - Journal of Endodontics
T1  - Effect of Aging and Carotid Atherosclerosis on Multifractality of Dental Pulp Blood Flow Oscillations
VL  - 46
IS  - 3
SP  - 358
EP  - 363
DO  - 10.1016/j.joen.2019.12.008
ER  - 

@article{
author = "Dželetović, Bojan and Aleksić, Nikola and Radak, Đorđe and Stratimirović, Đorđe and Đukić, Ljiljana and Stojić, Dragica",
year = "2020",
abstract = "Introduction: Age-related changes of dental pulp tissue and atherosclerosis of carotid arteries as its feeding arteries could influence the functionality of pulpal circulation. The objective of our study was to evaluate the effect of aging (physiological process) and carotid bifurcation atherosclerosis (pathologic process) on the pulpal microcirculatory system using multifractal analysis of the laser Doppler flowmetry signal. Methods: Three groups of 10 subjects were enrolled in the study: the young group (healthy subjects, 20-25 years), the middle-aged group (healthy subjects, 50-60 years), and the clinical group (subjects with carotid bifurcation atherosclerosis, 50-60 years). Pulpal blood flow (PBF) signals recorded by laser Doppler flowmetry were assessed by multifractal analysis that estimates Holder exponents of the signal. PBF levels, the average mean values, and the range of Holder exponents were obtained. Results: PBF levels were significantly higher in the young group compared with the middle-aged and clinical groups, and the difference between the middle-aged and clinical groups was not statistically significant. The range of the Holder exponents was narrower in the middle-aged and clinical groups than in the young group and narrower in the clinical group than in the middle-aged group. The average mean value of Holder exponents was significantly higher in the young group than in the middle-aged and clinical groups, whereas there was no significant difference between the middle-aged and clinical groups. Conclusions: Our study investigating the multifractality of the PBF signal showed that the aging process and carotid atherosclerosis could affect the complex structure of PBF oscillations and contribute to a better understanding of pulpal hemodynamics.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Endodontics",
title = "Effect of Aging and Carotid Atherosclerosis on Multifractality of Dental Pulp Blood Flow Oscillations",
volume = "46",
number = "3",
pages = "358-363",
doi = "10.1016/j.joen.2019.12.008"
}

Dželetović, B., Aleksić, N., Radak, Đ., Stratimirović, Đ., Đukić, L.,& Stojić, D.. (2020). Effect of Aging and Carotid Atherosclerosis on Multifractality of Dental Pulp Blood Flow Oscillations. in Journal of Endodontics
Elsevier Science Inc, New York., 46(3), 358-363.
https://doi.org/10.1016/j.joen.2019.12.008

Dželetović B, Aleksić N, Radak Đ, Stratimirović Đ, Đukić L, Stojić D. Effect of Aging and Carotid Atherosclerosis on Multifractality of Dental Pulp Blood Flow Oscillations. in Journal of Endodontics. 2020;46(3):358-363.
doi:10.1016/j.joen.2019.12.008 .

Dželetović, Bojan, Aleksić, Nikola, Radak, Đorđe, Stratimirović, Đorđe, Đukić, Ljiljana, Stojić, Dragica, "Effect of Aging and Carotid Atherosclerosis on Multifractality of Dental Pulp Blood Flow Oscillations" in Journal of Endodontics, 46, no. 3 (2020):358-363,
https://doi.org/10.1016/j.joen.2019.12.008 . .

TY  - JOUR
AU  - Dželetović, Bojan
AU  - Stratimirović, Đorđe
AU  - Stojić, Dragica
AU  - Đukić, Ljiljana
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1049
AB  - Aim To investigate the effect of ageing on control mechanisms of pulpal microcirculation using wavelet analysis and to calculate linear and nonlinear parameters of blood flow oscillations, in a healthy general population. Methodology Pulpal blood flow (PBF) oscillations were recorded on right maxillary central incisors using laser Doppler Flowmeter (PeriFlux PF 5001, Perimed, Jarfalla, Sweden) on a group of 10 young participants (20-25 years) and a group of 10 older adults (60-70 years). In total, 20 recordings were obtained for at least 20 min (one recording on one tooth per subject). Using wavelet spectral analysis, the amplitude and power were calculated as a linear and Hurst exponent as a nonlinear parameter of PBF oscillations. Differences between the two groups were assessed with the independent Student t-test. Results Mean PBF levels were significantly lower (P = 0.024) in older adults than in young participants. Relative amplitudes and powers corresponding to the myogenic (P = 0.046, P  lt  0.001, respectively) and neurogenic activity (P = 0.04, P = 0.01, respectively) were significantly higher, whereas values corresponding to the endothelial function (P = 0.04, P = 0.01, respectively) were significantly lower in older adults than in young participants. Hurst exponents of the total spectrum, myogenic and endothelial component (P  lt  0.001, P = 0.02, P  lt  0.001, respectively) of PBF oscillations were significantly lower in older adults in comparison to young participants. Conclusions At the level of pulpal microcirculation, ageing was associated with altered blood flow levels, the contribution of different control mechanisms to blood flow oscillations as well as the interaction of vascular smooth muscle and endothelium. Described changes of pulpal haemodynamics contribute to a better understanding of physiological behaviour and decreased adaptability of aged dental pulp to pathological stimuli.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Linear and nonlinear analysis of dental pulp blood flow oscillations in ageing
DO  - 10.1111/iej.13306
ER  - 

@article{
author = "Dželetović, Bojan and Stratimirović, Đorđe and Stojić, Dragica and Đukić, Ljiljana",
year = "2020",
abstract = "Aim To investigate the effect of ageing on control mechanisms of pulpal microcirculation using wavelet analysis and to calculate linear and nonlinear parameters of blood flow oscillations, in a healthy general population. Methodology Pulpal blood flow (PBF) oscillations were recorded on right maxillary central incisors using laser Doppler Flowmeter (PeriFlux PF 5001, Perimed, Jarfalla, Sweden) on a group of 10 young participants (20-25 years) and a group of 10 older adults (60-70 years). In total, 20 recordings were obtained for at least 20 min (one recording on one tooth per subject). Using wavelet spectral analysis, the amplitude and power were calculated as a linear and Hurst exponent as a nonlinear parameter of PBF oscillations. Differences between the two groups were assessed with the independent Student t-test. Results Mean PBF levels were significantly lower (P = 0.024) in older adults than in young participants. Relative amplitudes and powers corresponding to the myogenic (P = 0.046, P  lt  0.001, respectively) and neurogenic activity (P = 0.04, P = 0.01, respectively) were significantly higher, whereas values corresponding to the endothelial function (P = 0.04, P = 0.01, respectively) were significantly lower in older adults than in young participants. Hurst exponents of the total spectrum, myogenic and endothelial component (P  lt  0.001, P = 0.02, P  lt  0.001, respectively) of PBF oscillations were significantly lower in older adults in comparison to young participants. Conclusions At the level of pulpal microcirculation, ageing was associated with altered blood flow levels, the contribution of different control mechanisms to blood flow oscillations as well as the interaction of vascular smooth muscle and endothelium. Described changes of pulpal haemodynamics contribute to a better understanding of physiological behaviour and decreased adaptability of aged dental pulp to pathological stimuli.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Linear and nonlinear analysis of dental pulp blood flow oscillations in ageing",
doi = "10.1111/iej.13306"
}

Dželetović, B., Stratimirović, Đ., Stojić, D.,& Đukić, L.. (2020). Linear and nonlinear analysis of dental pulp blood flow oscillations in ageing. in International Endodontic Journal
Wiley, Hoboken..
https://doi.org/10.1111/iej.13306

Dželetović B, Stratimirović Đ, Stojić D, Đukić L. Linear and nonlinear analysis of dental pulp blood flow oscillations in ageing. in International Endodontic Journal. 2020;.
doi:10.1111/iej.13306 .

Dželetović, Bojan, Stratimirović, Đorđe, Stojić, Dragica, Đukić, Ljiljana, "Linear and nonlinear analysis of dental pulp blood flow oscillations in ageing" in International Endodontic Journal (2020),
https://doi.org/10.1111/iej.13306 . .

TY  - JOUR
AU  - Vučetić, Milan
AU  - Roganović, Jelena
AU  - Freilich, M.
AU  - Shafer, D.
AU  - Milić, Marija
AU  - Đukić, Ljiljana
AU  - Petrović, Nina
AU  - Marković, Evgenija
AU  - Marković, Aleksa
AU  - Brković, Božidar
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2481
AB  - Objective: To evaluate an association between bone levels of inflammation/oxidative stress mediators and postoperative discomfort after third molar conventional or piezosurgery. Material and methods: Twenty-six subjects with bilaterally impacted mandibular third molars, who underwent either piezo or conventional surgery, were included in a split-mouth design study. MicroRNA-21 (miR-21) expression, interleukin-1 beta (IL-1β), and vascular endothelial growth factor (VEGF) proteins, as well as superoxide dismutase (SOD) activity in alveolar bone, were evaluated. Pain intensity, the first pain appearance, analgesic first use and total dose taken, trismus, and swelling were clinically recorded. Results: MiR-21 expression was higher while VEGF protein was lower in piezosurgery vs. conventional groups. The differences in IL-1β protein and SOD activity were not significant between groups. The pain intensity on the first day was significantly decreased in piezosurgery group. The first pain appearance and the first analgesic taken were reported sooner in conventional vs. piezosurgical group. Significantly pronounced trismus on the third day following conventional surgery was found. In conventional group, significantly increased trismus was observed on the third compared to the first postoperative day. MiR-21 showed significant correlation with the first pain appearance. Conclusion: Delayed onset of less pronounced postoperative pain after piezosurgical vs. conventional extraction of impacted lower third molar was significantly associated with expression of bone miR-21. Clinical relevance: Alveolar bone miR-21 may reflect surgical stress and is associated with third molar postoperative pain onset.
PB  - Springer
T2  - Clinical Oral Investigations
T1  - Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort
DO  - 10.1007/s00784-020-03366-6
ER  - 

@article{
author = "Vučetić, Milan and Roganović, Jelena and Freilich, M. and Shafer, D. and Milić, Marija and Đukić, Ljiljana and Petrović, Nina and Marković, Evgenija and Marković, Aleksa and Brković, Božidar",
year = "2020",
abstract = "Objective: To evaluate an association between bone levels of inflammation/oxidative stress mediators and postoperative discomfort after third molar conventional or piezosurgery. Material and methods: Twenty-six subjects with bilaterally impacted mandibular third molars, who underwent either piezo or conventional surgery, were included in a split-mouth design study. MicroRNA-21 (miR-21) expression, interleukin-1 beta (IL-1β), and vascular endothelial growth factor (VEGF) proteins, as well as superoxide dismutase (SOD) activity in alveolar bone, were evaluated. Pain intensity, the first pain appearance, analgesic first use and total dose taken, trismus, and swelling were clinically recorded. Results: MiR-21 expression was higher while VEGF protein was lower in piezosurgery vs. conventional groups. The differences in IL-1β protein and SOD activity were not significant between groups. The pain intensity on the first day was significantly decreased in piezosurgery group. The first pain appearance and the first analgesic taken were reported sooner in conventional vs. piezosurgical group. Significantly pronounced trismus on the third day following conventional surgery was found. In conventional group, significantly increased trismus was observed on the third compared to the first postoperative day. MiR-21 showed significant correlation with the first pain appearance. Conclusion: Delayed onset of less pronounced postoperative pain after piezosurgical vs. conventional extraction of impacted lower third molar was significantly associated with expression of bone miR-21. Clinical relevance: Alveolar bone miR-21 may reflect surgical stress and is associated with third molar postoperative pain onset.",
publisher = "Springer",
journal = "Clinical Oral Investigations",
title = "Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort",
doi = "10.1007/s00784-020-03366-6"
}

Vučetić, M., Roganović, J., Freilich, M., Shafer, D., Milić, M., Đukić, L., Petrović, N., Marković, E., Marković, A.,& Brković, B.. (2020). Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort. in Clinical Oral Investigations
Springer..
https://doi.org/10.1007/s00784-020-03366-6

Vučetić M, Roganović J, Freilich M, Shafer D, Milić M, Đukić L, Petrović N, Marković E, Marković A, Brković B. Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort. in Clinical Oral Investigations. 2020;.
doi:10.1007/s00784-020-03366-6 .

Vučetić, Milan, Roganović, Jelena, Freilich, M., Shafer, D., Milić, Marija, Đukić, Ljiljana, Petrović, Nina, Marković, Evgenija, Marković, Aleksa, Brković, Božidar, "Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort" in Clinical Oral Investigations (2020),
https://doi.org/10.1007/s00784-020-03366-6 . .

TY  - JOUR
AU  - Krunić, Jelena
AU  - Stojanović, Nikola
AU  - Đukić, Ljiljana
AU  - Roganović, Jelena
AU  - Popović, Branka
AU  - Simić, Ivana
AU  - Stojić, Dragica
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2465
AB  - ObjectivesTo evaluate local effect of gaseous ozone on bacteria in deep carious lesions after incomplete caries removal, using chlorhexidine as control, and to investigate its effect on pulp vascular endothelial growth factor (VEGF), neuronal nitric oxide synthase (nNOS), and superoxide dismutase (SOD).Materials and methodsAntibacterial effect was evaluated in 48 teeth with diagnosed deep carious lesion. After incomplete caries removal, teeth were randomly allocated into two groups regarding the cavity disinfectant used: ozone (open system) or 2% chlorhexidine. Dentin samples were analyzed for the presence of total bacteria and Lactobacillus spp. by real-time quantitative polymerase chain reaction. For evaluation of ozone effect on dental pulp, 38 intact permanent teeth indicated for pulp removal/tooth extraction were included. After cavity preparation, teeth were randomly allocated into two groups: ozone group and control group. VEGF/nNOS level and SOD activity in dental pulp were determined by enzyme-linked immunosorbent assay and spectrophotometric method, respectively.ResultsOzone application decreased number of total bacteria (p=0.001) and Lactobacillus spp. (p lt 0.001), similarly to chlorhexidine. The VEGF (p lt 0.001) and nNOS (p=0.012) levels in dental pulp after ozone application were higher, while SOD activity was lower (p=0.001) comparing to those in control pulp.ConclusionsAntibacterial effect of ozone on residual bacteria after incomplete caries removal was similar to that of 2% chlorhexidine. Effect of ozone on pulp VEGF, nNOS, and SOD indicated its biocompatibility.Clinical relevanceOzone appears as effective and biocompatible cavity disinfectant in treatment of deep carious lesions by incomplete caries removal technique.
PB  - Springer Heidelberg, Heidelberg
T2  - Clinical Oral Investigations
T1  - Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal
VL  - 23
IS  - 2
SP  - 785
EP  - 792
DO  - 10.1007/s00784-018-2495-x
ER  - 

@article{
author = "Krunić, Jelena and Stojanović, Nikola and Đukić, Ljiljana and Roganović, Jelena and Popović, Branka and Simić, Ivana and Stojić, Dragica",
year = "2019",
abstract = "ObjectivesTo evaluate local effect of gaseous ozone on bacteria in deep carious lesions after incomplete caries removal, using chlorhexidine as control, and to investigate its effect on pulp vascular endothelial growth factor (VEGF), neuronal nitric oxide synthase (nNOS), and superoxide dismutase (SOD).Materials and methodsAntibacterial effect was evaluated in 48 teeth with diagnosed deep carious lesion. After incomplete caries removal, teeth were randomly allocated into two groups regarding the cavity disinfectant used: ozone (open system) or 2% chlorhexidine. Dentin samples were analyzed for the presence of total bacteria and Lactobacillus spp. by real-time quantitative polymerase chain reaction. For evaluation of ozone effect on dental pulp, 38 intact permanent teeth indicated for pulp removal/tooth extraction were included. After cavity preparation, teeth were randomly allocated into two groups: ozone group and control group. VEGF/nNOS level and SOD activity in dental pulp were determined by enzyme-linked immunosorbent assay and spectrophotometric method, respectively.ResultsOzone application decreased number of total bacteria (p=0.001) and Lactobacillus spp. (p lt 0.001), similarly to chlorhexidine. The VEGF (p lt 0.001) and nNOS (p=0.012) levels in dental pulp after ozone application were higher, while SOD activity was lower (p=0.001) comparing to those in control pulp.ConclusionsAntibacterial effect of ozone on residual bacteria after incomplete caries removal was similar to that of 2% chlorhexidine. Effect of ozone on pulp VEGF, nNOS, and SOD indicated its biocompatibility.Clinical relevanceOzone appears as effective and biocompatible cavity disinfectant in treatment of deep carious lesions by incomplete caries removal technique.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Clinical Oral Investigations",
title = "Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal",
volume = "23",
number = "2",
pages = "785-792",
doi = "10.1007/s00784-018-2495-x"
}

Krunić, J., Stojanović, N., Đukić, L., Roganović, J., Popović, B., Simić, I.,& Stojić, D.. (2019). Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal. in Clinical Oral Investigations
Springer Heidelberg, Heidelberg., 23(2), 785-792.
https://doi.org/10.1007/s00784-018-2495-x

Krunić J, Stojanović N, Đukić L, Roganović J, Popović B, Simić I, Stojić D. Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal. in Clinical Oral Investigations. 2019;23(2):785-792.
doi:10.1007/s00784-018-2495-x .

Krunić, Jelena, Stojanović, Nikola, Đukić, Ljiljana, Roganović, Jelena, Popović, Branka, Simić, Ivana, Stojić, Dragica, "Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal" in Clinical Oral Investigations, 23, no. 2 (2019):785-792,
https://doi.org/10.1007/s00784-018-2495-x . .

TY  - JOUR
AU  - Baćević, Miljana
AU  - Brković, Božidar
AU  - Lamber, France
AU  - Đukić, Ljiljana
AU  - Petrović, Nina
AU  - Roganović, Jelena
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2388
AB  - Objective: Leukocyte- and platelet-rich fibrin (L-PRF) represents a natural, low-cost product which may promote tissue healing by mechanisms not fully elucidated. Diabetes mellitus (DM) disrupts bone healing by inducing inflammation and oxidative stress (OS), mechanisms regulated by microRNAs (miRs). The aim of the present study was to investigate the microRNA-21 (miR-21) involvement in diabetic bone regeneration using L-PRF alone or in combination with a standard grafting material. Design: After the induction of diabetes (alloxan 100 mg/kg), four cranial osteotomies were made in diabetic (n = 12) and non-diabetic (n = 12) rabbits: one was left empty and the remaining three were grafted with L-PRF, bovine hydroxyapatite (Bio-Oss((R))) and L-PRF + Bio-Oss((R)). Two and eight weeks postoperatively, the samples were harvested for miR-21 expression (Real-time RT-PCR) and enzyme-linked immunosorbent assay analyses. Results: Diabetic rabbits showed decreased miR-21 and matrix metalloproteinase-9 (MMP-9) protein expression while increased malondialdehyde (MDA) levels two weeks postoperatively; however, there were no significant differences in miR-21 and MMP-9 levels between diabetic and non-diabetic rabbits in samples taken eight weeks postoperatively. Application of L-PRF and L-PRF + Bio-Oss((R)) improved miR-21 and MMP-9 and decreased MDA levels while Bio-Oss((R)) alone enhanced superoxide dismutase (SOD) activity levels in diabetic rabbits. Conclusion: L-PRF alone or in combination with bovine hydroxyapatite as bone graft could be beneficial in DM since it seems to improve inflammation-modulatory miR-21 expression and decreases oxidative stress.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Archives of Oral Biology
T1  - Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits
VL  - 102
SP  - 231
EP  - 237
DO  - 10.1016/j.archoralbio.2019.05.005
ER  - 

@article{
author = "Baćević, Miljana and Brković, Božidar and Lamber, France and Đukić, Ljiljana and Petrović, Nina and Roganović, Jelena",
year = "2019",
abstract = "Objective: Leukocyte- and platelet-rich fibrin (L-PRF) represents a natural, low-cost product which may promote tissue healing by mechanisms not fully elucidated. Diabetes mellitus (DM) disrupts bone healing by inducing inflammation and oxidative stress (OS), mechanisms regulated by microRNAs (miRs). The aim of the present study was to investigate the microRNA-21 (miR-21) involvement in diabetic bone regeneration using L-PRF alone or in combination with a standard grafting material. Design: After the induction of diabetes (alloxan 100 mg/kg), four cranial osteotomies were made in diabetic (n = 12) and non-diabetic (n = 12) rabbits: one was left empty and the remaining three were grafted with L-PRF, bovine hydroxyapatite (Bio-Oss((R))) and L-PRF + Bio-Oss((R)). Two and eight weeks postoperatively, the samples were harvested for miR-21 expression (Real-time RT-PCR) and enzyme-linked immunosorbent assay analyses. Results: Diabetic rabbits showed decreased miR-21 and matrix metalloproteinase-9 (MMP-9) protein expression while increased malondialdehyde (MDA) levels two weeks postoperatively; however, there were no significant differences in miR-21 and MMP-9 levels between diabetic and non-diabetic rabbits in samples taken eight weeks postoperatively. Application of L-PRF and L-PRF + Bio-Oss((R)) improved miR-21 and MMP-9 and decreased MDA levels while Bio-Oss((R)) alone enhanced superoxide dismutase (SOD) activity levels in diabetic rabbits. Conclusion: L-PRF alone or in combination with bovine hydroxyapatite as bone graft could be beneficial in DM since it seems to improve inflammation-modulatory miR-21 expression and decreases oxidative stress.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Archives of Oral Biology",
title = "Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits",
volume = "102",
pages = "231-237",
doi = "10.1016/j.archoralbio.2019.05.005"
}

Baćević, M., Brković, B., Lamber, F., Đukić, L., Petrović, N.,& Roganović, J.. (2019). Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits. in Archives of Oral Biology
Pergamon-Elsevier Science Ltd, Oxford., 102, 231-237.
https://doi.org/10.1016/j.archoralbio.2019.05.005

Baćević M, Brković B, Lamber F, Đukić L, Petrović N, Roganović J. Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits. in Archives of Oral Biology. 2019;102:231-237.
doi:10.1016/j.archoralbio.2019.05.005 .

Baćević, Miljana, Brković, Božidar, Lamber, France, Đukić, Ljiljana, Petrović, Nina, Roganović, Jelena, "Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits" in Archives of Oral Biology, 102 (2019):231-237,
https://doi.org/10.1016/j.archoralbio.2019.05.005 . .

TY  - JOUR
AU  - Milosavljević, A.
AU  - Đukić, Ljiljana
AU  - Toljić, Boško
AU  - Milašin, Jelena
AU  - Dželetović, Bojan
AU  - Brković, Božidar
AU  - Roganović, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2321
AB  - AimTo investigate melatonin (MEL) levels in human dental pulp tissue (hDP) in type 2 diabetic (T2D) participants and the underlying molecular mechanisms of its effects in human dental pulp cells (hDPCs) under hyperglycaemia. MethodologyThe study included 16 healthy and 16 T2D participants who underwent vital pulp extirpation for hDP and four healthy participants undergoing third molar extraction for hDPCs analyses. MTT and NRU were used as tests for cytotoxicity. The pulp tissue levels of MEL, inducible NO synthase (iNOS) and superoxide dismutase (SOD) activity, as well as iNOS, histone acetyltransferase p300 (p300) and SOD activity levels in hDPCs incubated with MEL (0.1 and 1.0mmolL(-1)) under normoglycaemia and hyperglycaemia were measured by enzyme-linked immunosorbent assay. Comparisons between the two groups were made by unpaired t-tests or Mann-Whitney test whilst the chi-square test was used for dichotomous variables. To compare more groups, the Kruskal-Wallis test with Dunn's multiple comparison was used, whilst Spearman correlation was used to assess association between two variables. ResultsMelatonin was decreased (124.3021.6 vs. 240.0 +/- 19.1 pgmL(-1), P lt 0.01), whilst iNOS levels increased (0.92 +/- 0.08 vs. 0.32 +/- 0.09ngmL(-1), P lt 0.01) in hDP from T2D compared to nondiabetic participants. In hDPCs, MEL (0.1 and 1.0mmolL(-1)) had no cytotoxicity. Incubation with 1.0mmolL(-1) of MEL (24h) decreased hyperglycaemia-induced increases of iNOS (0.34 +/- 0.01ngmL(-1) vs. 0.40 +/- 0.01ngmL(-1), P lt 0.01) and p300 (11.59 +/- 0.58ngmL(-1) vs. 16.12 +/- 0.39ngmL(-1), P lt 0.01), and also, increased SOD activity (87.11 +/- 3.10% vs. 68.56 +/- 3.77%, P lt 0.01) to the levels comparable to the normoglycaemic; iNOS and p300 protein expression levels showed strong positive correlation under hyperglycaemia (Spearman r=0.8242, P lt 0.001). ConclusionType 2 diabetic participants had decreased MEL in hDP. At pharmacological concentrations, MEL is not cytotoxic for hDPCs and normalizes iNOS and SOD activity levels in hyperglyceamic hDPCs suggesting its antioxidant and protective effects in human dental pulp tissue under hyperglycaemia.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions
VL  - 51
IS  - 10
SP  - 1149
EP  - 1158
DO  - 10.1111/iej.12934
ER  - 

@article{
author = "Milosavljević, A. and Đukić, Ljiljana and Toljić, Boško and Milašin, Jelena and Dželetović, Bojan and Brković, Božidar and Roganović, Jelena",
year = "2018",
abstract = "AimTo investigate melatonin (MEL) levels in human dental pulp tissue (hDP) in type 2 diabetic (T2D) participants and the underlying molecular mechanisms of its effects in human dental pulp cells (hDPCs) under hyperglycaemia. MethodologyThe study included 16 healthy and 16 T2D participants who underwent vital pulp extirpation for hDP and four healthy participants undergoing third molar extraction for hDPCs analyses. MTT and NRU were used as tests for cytotoxicity. The pulp tissue levels of MEL, inducible NO synthase (iNOS) and superoxide dismutase (SOD) activity, as well as iNOS, histone acetyltransferase p300 (p300) and SOD activity levels in hDPCs incubated with MEL (0.1 and 1.0mmolL(-1)) under normoglycaemia and hyperglycaemia were measured by enzyme-linked immunosorbent assay. Comparisons between the two groups were made by unpaired t-tests or Mann-Whitney test whilst the chi-square test was used for dichotomous variables. To compare more groups, the Kruskal-Wallis test with Dunn's multiple comparison was used, whilst Spearman correlation was used to assess association between two variables. ResultsMelatonin was decreased (124.3021.6 vs. 240.0 +/- 19.1 pgmL(-1), P lt 0.01), whilst iNOS levels increased (0.92 +/- 0.08 vs. 0.32 +/- 0.09ngmL(-1), P lt 0.01) in hDP from T2D compared to nondiabetic participants. In hDPCs, MEL (0.1 and 1.0mmolL(-1)) had no cytotoxicity. Incubation with 1.0mmolL(-1) of MEL (24h) decreased hyperglycaemia-induced increases of iNOS (0.34 +/- 0.01ngmL(-1) vs. 0.40 +/- 0.01ngmL(-1), P lt 0.01) and p300 (11.59 +/- 0.58ngmL(-1) vs. 16.12 +/- 0.39ngmL(-1), P lt 0.01), and also, increased SOD activity (87.11 +/- 3.10% vs. 68.56 +/- 3.77%, P lt 0.01) to the levels comparable to the normoglycaemic; iNOS and p300 protein expression levels showed strong positive correlation under hyperglycaemia (Spearman r=0.8242, P lt 0.001). ConclusionType 2 diabetic participants had decreased MEL in hDP. At pharmacological concentrations, MEL is not cytotoxic for hDPCs and normalizes iNOS and SOD activity levels in hyperglyceamic hDPCs suggesting its antioxidant and protective effects in human dental pulp tissue under hyperglycaemia.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions",
volume = "51",
number = "10",
pages = "1149-1158",
doi = "10.1111/iej.12934"
}

Milosavljević, A., Đukić, L., Toljić, B., Milašin, J., Dželetović, B., Brković, B.,& Roganović, J.. (2018). Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions. in International Endodontic Journal
Wiley, Hoboken., 51(10), 1149-1158.
https://doi.org/10.1111/iej.12934

Milosavljević A, Đukić L, Toljić B, Milašin J, Dželetović B, Brković B, Roganović J. Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions. in International Endodontic Journal. 2018;51(10):1149-1158.
doi:10.1111/iej.12934 .

Milosavljević, A., Đukić, Ljiljana, Toljić, Boško, Milašin, Jelena, Dželetović, Bojan, Brković, Božidar, Roganović, Jelena, "Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions" in International Endodontic Journal, 51, no. 10 (2018):1149-1158,
https://doi.org/10.1111/iej.12934 . .

TY  - THES
AU  - Đukić, Ljiljana
PY  - 2016
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=3755
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:12653/bdef:Content/download
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:12766/bdef:Izvestaj/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48104975
UR  - http://nardus.mpn.gov.rs/123456789/6436
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1016
AB  - Introduction: Saliva is a medium which, due to its components, flow rate and antioxidant capacity, greatly contributes to the oral homeostasis. The most common factors causing reduced salivary flow rate and/or xerostomia (subjective feeling of dry mouth) are adverse oral reactions of drugs, such as antihypertensives, and diseases, such as diabetes mellitus (DM) type 2. ACE inhibitors are the first-line antihypertensive drugs for the control of essential hypertension and hypertension with DM type 2. For the time being, there are no well-controled clinical trials showing xerogenic effect of ACE inhibitors. Beside ACE inhibitors direct effect on angiotensin system components, their protective effects (antioxidant, antiproliferative, antithrombotic) also significantly contribute to the beneficial therapeutic effects of these drugs. However, for the moment there are no data regarding mentioned effects of ACE inhibitors in human salivary glands and saliva. Aims: This study aimed: (1) to determine unstimulated whole saliva (UWS) flow rate in hypertensive patients with and without DM type 2 on enalapril (ACE inhibitor) treatment, as well as in healthy subjects; (2) to determine the presence of xerostomia in hypertensive patients with and without DM type 2 on enalapril treatment, as well as in healthy subjects; (3) to determine ACE and ACE2 presence and concentration in UWS sampled one hour before (minimal concentrations) and 4 hours after (maximal concentrations) enalapril morning dose intake in hypertensive patients with and without DM type 2 on enalapril treatment, as well as in healthy subjects; (4) to determine concentrations of malondialdehyde (MDA) adducts, marker of oxidative stress-lipid peroxidation, in UWS sampled one hour before and 4 hours after enalapril morning dose intake in hypertensive patients with and without DM type 2 on enalapril treatment, as well as in healthy subjects; (5) to determine antioxidant capacity by measuring total antioxidant capacity (TAC) and total glutathione concentration, as well as superoxide dismutase (SOD) activity in UWS sampled one hour before and 4 hours after enalapril morning dose intake in hypertensive patients with and without DM type 2 on enalapril treatment, as well as in healthy subjects; (6) to determine presence of angiotensin system components (ACE, ACE2, AT1 receptors) in human parotid gland tissues; (7) to determine eNOS and iNOS presence and concentrations in UWS sampled one hour before and 4 hours after enalapril morning dose intake in hypertensive patients with and without DM type 2 on enalapril treatment, as well as in healthy subjects...
AB  - Uvod: Pljuvaĉka je medijum koji svojim komponentama, protokom i antioksidativnim kapacitetom, u velikoj meri doprinosi oralnoj homeostazi. Najĉešći faktori odgovorni za nastanak smanjenog luĉenja pljuvaĉke i/ili kserostomije (subjektivni osećaj suvoće usta) su neţeljeni efekti lekova, kao što su antihipertenzivi, i bolesti, kao što je dijabetes melitus (DM) tip 2. ACE inhibitori danas predstavljaju lekove prvog izbora za leĉenje, kako esencijalne hipertenzije, tako i hipertenzije udruţene sa DM tip 2. Za sada nema dobro dokumentovanih kliniĉkih studija koje direktno ukazuju na kserogeni efekat ACE inhibitora. Povoljnom terapijskom efektu ACE inhibitora, pored njihovog direktnog dejstva na komponente angiotenzinskog sistema, znaĉajno doprinose i njihovi protektivni efekti (antioksidativni, antiproliferativni, antioagregacioni). O pomenutim efektima ACE inhibitora na nivou pljuvaĉnih ţlezda i pljuvaĉke kod ljudi za sada nema podataka. Ciljevi: 1. Odrediti protok ukupne nestimulisane pljuvaĉke (UNP) kod pacijenata sa hipertenzijom i hipertenzijom sa DM tip 2 na terapiji enalaprilom (ACE inhibitor), kao i kod zdravih osoba; 2. Utvrditi prisustvo subjektivanog osećaja suvoće usta kod pacijenata sa hipertenzijom i hipertenzijom sa DM tip 2 na terapiji enalaprilom, kao i kod zdravih osoba; 3. Odrediti prisustvo i koncentracije ACE i ACE2 u UNP prikupljenoj 1 sat pre (minimalne koncentracije) i 4 sata posle (maksimalne koncentracije) primene jutarnje terapijske doze enalaprila kod pacijenata sa hipertenzijom i hipertenzijom sa DM tip 2 na terapiji ovim lekom, kao i kod zdravih osoba; 4. Odrediti stepen oksidativnog stresa na nivou lipidne peroksidacije merenjem malondialdehida (MDA) vezanog za proteine u UNP prikupljenoj 1 sat pre i 4 sata posle primene jutarnje terapijske doze enalaprila kod pacijenata sa hipertenzijom i hipertenzijom sa DM tip 2 na terapiji ovim lekom, kao i kod zdravih osoba; 5. Odrediti stepen antioksidativnog kapaciteta merenjem totalnog antioksidativnog kapaciteta (TAC), koncentracija ukupnog glutationa i aktivnosti superoksid dizmutaze - SOD u UNP prikupljenoj 1 sat pre i 4 sata posle primene jutarnje terapijske doze enalaprila kod pacijenata sa hipertenzijom i hipertenzijom sa DM tip 2 na terapiji ovim lekom, kao i kod zdravih osoba; 6. Utvrditi prisustvo komponenti angiotenzinskog sistema u tkivu parotidne pljuvaĉne ţlezde ĉoveka: ACE, ACE2 i receptor za angiotenzin II tip 1 (AT1 receptor); 7...
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - ACE inhibitors, enalapril, and mechanisms of oral homeostasis: salivary flow rate and antioxidant capacity
T1  - ACE inhibitori, enalapril, i mehanizmi oralne homeostaze: protok pljuvačke i antioksidativna zaštita
UR  - https://hdl.handle.net/21.15107/rcub_nardus_6436
ER  - 

@phdthesis{
author = "Đukić, Ljiljana",
year = "2016",
abstract = "Introduction: Saliva is a medium which, due to its components, flow rate and antioxidant capacity, greatly contributes to the oral homeostasis. The most common factors causing reduced salivary flow rate and/or xerostomia (subjective feeling of dry mouth) are adverse oral reactions of drugs, such as antihypertensives, and diseases, such as diabetes mellitus (DM) type 2. ACE inhibitors are the first-line antihypertensive drugs for the control of essential hypertension and hypertension with DM type 2. For the time being, there are no well-controled clinical trials showing xerogenic effect of ACE inhibitors. Beside ACE inhibitors direct effect on angiotensin system components, their protective effects (antioxidant, antiproliferative, antithrombotic) also significantly contribute to the beneficial therapeutic effects of these drugs. However, for the moment there are no data regarding mentioned effects of ACE inhibitors in human salivary glands and saliva. Aims: This study aimed: (1) to determine unstimulated whole saliva (UWS) flow rate in hypertensive patients with and without DM type 2 on enalapril (ACE inhibitor) treatment, as well as in healthy subjects; (2) to determine the presence of xerostomia in hypertensive patients with and without DM type 2 on enalapril treatment, as well as in healthy subjects; (3) to determine ACE and ACE2 presence and concentration in UWS sampled one hour before (minimal concentrations) and 4 hours after (maximal concentrations) enalapril morning dose intake in hypertensive patients with and without DM type 2 on enalapril treatment, as well as in healthy subjects; (4) to determine concentrations of malondialdehyde (MDA) adducts, marker of oxidative stress-lipid peroxidation, in UWS sampled one hour before and 4 hours after enalapril morning dose intake in hypertensive patients with and without DM type 2 on enalapril treatment, as well as in healthy subjects; (5) to determine antioxidant capacity by measuring total antioxidant capacity (TAC) and total glutathione concentration, as well as superoxide dismutase (SOD) activity in UWS sampled one hour before and 4 hours after enalapril morning dose intake in hypertensive patients with and without DM type 2 on enalapril treatment, as well as in healthy subjects; (6) to determine presence of angiotensin system components (ACE, ACE2, AT1 receptors) in human parotid gland tissues; (7) to determine eNOS and iNOS presence and concentrations in UWS sampled one hour before and 4 hours after enalapril morning dose intake in hypertensive patients with and without DM type 2 on enalapril treatment, as well as in healthy subjects..., Uvod: Pljuvaĉka je medijum koji svojim komponentama, protokom i antioksidativnim kapacitetom, u velikoj meri doprinosi oralnoj homeostazi. Najĉešći faktori odgovorni za nastanak smanjenog luĉenja pljuvaĉke i/ili kserostomije (subjektivni osećaj suvoće usta) su neţeljeni efekti lekova, kao što su antihipertenzivi, i bolesti, kao što je dijabetes melitus (DM) tip 2. ACE inhibitori danas predstavljaju lekove prvog izbora za leĉenje, kako esencijalne hipertenzije, tako i hipertenzije udruţene sa DM tip 2. Za sada nema dobro dokumentovanih kliniĉkih studija koje direktno ukazuju na kserogeni efekat ACE inhibitora. Povoljnom terapijskom efektu ACE inhibitora, pored njihovog direktnog dejstva na komponente angiotenzinskog sistema, znaĉajno doprinose i njihovi protektivni efekti (antioksidativni, antiproliferativni, antioagregacioni). O pomenutim efektima ACE inhibitora na nivou pljuvaĉnih ţlezda i pljuvaĉke kod ljudi za sada nema podataka. Ciljevi: 1. Odrediti protok ukupne nestimulisane pljuvaĉke (UNP) kod pacijenata sa hipertenzijom i hipertenzijom sa DM tip 2 na terapiji enalaprilom (ACE inhibitor), kao i kod zdravih osoba; 2. Utvrditi prisustvo subjektivanog osećaja suvoće usta kod pacijenata sa hipertenzijom i hipertenzijom sa DM tip 2 na terapiji enalaprilom, kao i kod zdravih osoba; 3. Odrediti prisustvo i koncentracije ACE i ACE2 u UNP prikupljenoj 1 sat pre (minimalne koncentracije) i 4 sata posle (maksimalne koncentracije) primene jutarnje terapijske doze enalaprila kod pacijenata sa hipertenzijom i hipertenzijom sa DM tip 2 na terapiji ovim lekom, kao i kod zdravih osoba; 4. Odrediti stepen oksidativnog stresa na nivou lipidne peroksidacije merenjem malondialdehida (MDA) vezanog za proteine u UNP prikupljenoj 1 sat pre i 4 sata posle primene jutarnje terapijske doze enalaprila kod pacijenata sa hipertenzijom i hipertenzijom sa DM tip 2 na terapiji ovim lekom, kao i kod zdravih osoba; 5. Odrediti stepen antioksidativnog kapaciteta merenjem totalnog antioksidativnog kapaciteta (TAC), koncentracija ukupnog glutationa i aktivnosti superoksid dizmutaze - SOD u UNP prikupljenoj 1 sat pre i 4 sata posle primene jutarnje terapijske doze enalaprila kod pacijenata sa hipertenzijom i hipertenzijom sa DM tip 2 na terapiji ovim lekom, kao i kod zdravih osoba; 6. Utvrditi prisustvo komponenti angiotenzinskog sistema u tkivu parotidne pljuvaĉne ţlezde ĉoveka: ACE, ACE2 i receptor za angiotenzin II tip 1 (AT1 receptor); 7...",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "ACE inhibitors, enalapril, and mechanisms of oral homeostasis: salivary flow rate and antioxidant capacity, ACE inhibitori, enalapril, i mehanizmi oralne homeostaze: protok pljuvačke i antioksidativna zaštita",
url = "https://hdl.handle.net/21.15107/rcub_nardus_6436"
}

Đukić, L.. (2016). ACE inhibitors, enalapril, and mechanisms of oral homeostasis: salivary flow rate and antioxidant capacity. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_6436

Đukić L. ACE inhibitors, enalapril, and mechanisms of oral homeostasis: salivary flow rate and antioxidant capacity. 2016;.
https://hdl.handle.net/21.15107/rcub_nardus_6436 .

Đukić, Ljiljana, "ACE inhibitors, enalapril, and mechanisms of oral homeostasis: salivary flow rate and antioxidant capacity" (2016),
https://hdl.handle.net/21.15107/rcub_nardus_6436 .

TY  - JOUR
AU  - Roganović, Jelena
AU  - Đukić, Ljiljana
AU  - Kršljak, Elena
AU  - Tanić, Nasta
AU  - Stojić, Dragica
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1963
AB  - ObjectivesThe influence of experimental diabetes (alloxan, 100mgkg(-1)) was studied on rabbit parotid gland function. Material and MethodsCarbachol-induced parotid secretion in vivo, and in vitro quantification of inducible nitric oxide synthase (iNOS) mRNA expression, by real-time RT-PCR, and activity of superoxide dismutase (SOD) and total antioxidant capacity (TAC) in commercial colorimetric assays were measured in parotid glands of non-diabetic and diabetic rabbits. ResultsCarbachol-induced dose-dependent increase in parotid secretion significantly reduced in diabetic rabbits. Functional studies in the presence of muscarinic receptor and nitric oxide synthase (NOS) antagonists revealed that in M-3 receptor-mediated carbachol secretion, nitric oxide, deriving mainly from neuronal NOS (nNOS) in control, and iNOS in diabetic rabbits, was involved. Also, upregulation of iNOS mRNA expression and enhanced SOD activity and TAC were detected in diabetic glands. ConclusionsOur data suggest that decreased M-3 receptor-mediated parotid secretion in diabetic rabbits appears to be due to alterations in NO signaling, mainly due to iNOS induction, accompanied by elevated antioxidant response.
PB  - Wiley, Hoboken
T2  - Oral Diseases
T1  - Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits
VL  - 21
IS  - 5
SP  - 634
EP  - 640
DO  - 10.1111/odi.12327
ER  - 

@article{
author = "Roganović, Jelena and Đukić, Ljiljana and Kršljak, Elena and Tanić, Nasta and Stojić, Dragica",
year = "2015",
abstract = "ObjectivesThe influence of experimental diabetes (alloxan, 100mgkg(-1)) was studied on rabbit parotid gland function. Material and MethodsCarbachol-induced parotid secretion in vivo, and in vitro quantification of inducible nitric oxide synthase (iNOS) mRNA expression, by real-time RT-PCR, and activity of superoxide dismutase (SOD) and total antioxidant capacity (TAC) in commercial colorimetric assays were measured in parotid glands of non-diabetic and diabetic rabbits. ResultsCarbachol-induced dose-dependent increase in parotid secretion significantly reduced in diabetic rabbits. Functional studies in the presence of muscarinic receptor and nitric oxide synthase (NOS) antagonists revealed that in M-3 receptor-mediated carbachol secretion, nitric oxide, deriving mainly from neuronal NOS (nNOS) in control, and iNOS in diabetic rabbits, was involved. Also, upregulation of iNOS mRNA expression and enhanced SOD activity and TAC were detected in diabetic glands. ConclusionsOur data suggest that decreased M-3 receptor-mediated parotid secretion in diabetic rabbits appears to be due to alterations in NO signaling, mainly due to iNOS induction, accompanied by elevated antioxidant response.",
publisher = "Wiley, Hoboken",
journal = "Oral Diseases",
title = "Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits",
volume = "21",
number = "5",
pages = "634-640",
doi = "10.1111/odi.12327"
}

Roganović, J., Đukić, L., Kršljak, E., Tanić, N.,& Stojić, D.. (2015). Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits. in Oral Diseases
Wiley, Hoboken., 21(5), 634-640.
https://doi.org/10.1111/odi.12327

Roganović J, Đukić L, Kršljak E, Tanić N, Stojić D. Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits. in Oral Diseases. 2015;21(5):634-640.
doi:10.1111/odi.12327 .

Roganović, Jelena, Đukić, Ljiljana, Kršljak, Elena, Tanić, Nasta, Stojić, Dragica, "Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits" in Oral Diseases, 21, no. 5 (2015):634-640,
https://doi.org/10.1111/odi.12327 . .

TY  - JOUR
AU  - Đukić, Ljiljana
AU  - Roganović, Jelena
AU  - Brajović, Milan
AU  - Bokonjić, Dejan
AU  - Stojić, Dragica
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1961
AB  - ObjectiveThe present cross-sectional study aimed to determine the effect of first-line anti-hypertensive drugs (enalapril, metoprolol, and combinations of enalapril with metoprolol and/or hydrochlorothiazide) on salivary gland function and salivary total antioxidant capacity (TAC) in hypertensive patients with/without diabetes mellitus (DM) type 2. Materials and MethodsSalivary gland function was measured as xerostomia (interview) and unstimulated whole saliva flow rate (UWSFR) in 447 subjects (387 hypertensive and 60 healthy). Salivary TAC was evaluated by spectrophotometric assay. ResultsEnalapril is not xerogenic, while metoprolol and drug combinations are. In the presence of DM type 2, all drugs, except metoprolol, had pronounced xerogenic effect. Binary logistic regression analysis found enalapril to be significantly associated with decreased risk of xerogenic effect development, while DM type 2 with increased risk. In the presence of enalapril in hypertensive patients with/without DM type 2 salivary TAC was similar to that in healthy subjects, while for metoprolol was reduced. ConclusionsEnalapril is not xerogenic but is antioxidant, which moderately reduces the risk of xerogenic effect development even in the presence of DM type 2. However, metoprolol and drug combinations exhibit xerogenic effect. In DM type 2, xerogenic effect of all drugs was pronounced except of metoprolol.
PB  - Wiley, Hoboken
T2  - Oral Diseases
T1  - The effects of anti-hypertensives and type 2 diabetes on salivary flow and total antioxidant capacity
VL  - 21
IS  - 5
SP  - 619
EP  - 625
DO  - 10.1111/odi.12325
ER  - 

@article{
author = "Đukić, Ljiljana and Roganović, Jelena and Brajović, Milan and Bokonjić, Dejan and Stojić, Dragica",
year = "2015",
abstract = "ObjectiveThe present cross-sectional study aimed to determine the effect of first-line anti-hypertensive drugs (enalapril, metoprolol, and combinations of enalapril with metoprolol and/or hydrochlorothiazide) on salivary gland function and salivary total antioxidant capacity (TAC) in hypertensive patients with/without diabetes mellitus (DM) type 2. Materials and MethodsSalivary gland function was measured as xerostomia (interview) and unstimulated whole saliva flow rate (UWSFR) in 447 subjects (387 hypertensive and 60 healthy). Salivary TAC was evaluated by spectrophotometric assay. ResultsEnalapril is not xerogenic, while metoprolol and drug combinations are. In the presence of DM type 2, all drugs, except metoprolol, had pronounced xerogenic effect. Binary logistic regression analysis found enalapril to be significantly associated with decreased risk of xerogenic effect development, while DM type 2 with increased risk. In the presence of enalapril in hypertensive patients with/without DM type 2 salivary TAC was similar to that in healthy subjects, while for metoprolol was reduced. ConclusionsEnalapril is not xerogenic but is antioxidant, which moderately reduces the risk of xerogenic effect development even in the presence of DM type 2. However, metoprolol and drug combinations exhibit xerogenic effect. In DM type 2, xerogenic effect of all drugs was pronounced except of metoprolol.",
publisher = "Wiley, Hoboken",
journal = "Oral Diseases",
title = "The effects of anti-hypertensives and type 2 diabetes on salivary flow and total antioxidant capacity",
volume = "21",
number = "5",
pages = "619-625",
doi = "10.1111/odi.12325"
}

Đukić, L., Roganović, J., Brajović, M., Bokonjić, D.,& Stojić, D.. (2015). The effects of anti-hypertensives and type 2 diabetes on salivary flow and total antioxidant capacity. in Oral Diseases
Wiley, Hoboken., 21(5), 619-625.
https://doi.org/10.1111/odi.12325

Đukić L, Roganović J, Brajović M, Bokonjić D, Stojić D. The effects of anti-hypertensives and type 2 diabetes on salivary flow and total antioxidant capacity. in Oral Diseases. 2015;21(5):619-625.
doi:10.1111/odi.12325 .

Đukić, Ljiljana, Roganović, Jelena, Brajović, Milan, Bokonjić, Dejan, Stojić, Dragica, "The effects of anti-hypertensives and type 2 diabetes on salivary flow and total antioxidant capacity" in Oral Diseases, 21, no. 5 (2015):619-625,
https://doi.org/10.1111/odi.12325 . .

TY  - JOUR
AU  - Roganović, Jelena
AU  - Petrović, Nina
AU  - Đukić, Ljiljana
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1902
AB  - Background/Aim. Atherosclerotic-occlusive changes could be observed in orofacial branches of the external carotid artery. Atherosclerosis-induced ischemia caused alteration in production and release of endothelial factors. The aim of this study was to investigate the influence of carotid artery occlusion (10, 30 and 60 min) on vascular effects of norepinephrine (NOR) and neuropeptide Y (NPY) in the isolated glandular branch of the rabbit facial artery, the main feeding artery for the submandibular gland. Method. Changes in isometric tension were recorded in organ bath studies with arterial rings, before and after carotid artery occlusion. Results. Concentrationdependent vasocontractile effect of NOR was significantly augmented after 30 and 60 min of carotid occlusion, but only in the rings with intact endothelium. Given alone, NPY showed no effect in isolated glandular branch of the rabbit facial artery, but enhanced NOR vasoconstriction in all the investigated rings. NOR vasocontractile effect enhancement in the presence of NPY was attenuated after 30 and 60 min of carotid occlusion. Also, enhancement of NOR vasoconstriction by NPY was significantly higher in endothelium-intact rings compared to endotheliumdenuded rings obtained after 30 and 60 min of carotid occlusion. Conclusion. The present investigation provides results of increased vasocontractile effect of NOR and decreased enhancing effect of NPY on NOR vasoconstriction in the rabbit facial artery after carotid occlusion that is related to altered endothelium function.
AB  - Uvod/Cilj. Pokazano je da se okluzivne promene aterosklerotične prirode mogu opaziti na orofacijalnim granama spoljne karotidne arterije. Ishemija izazvana aterosklerozom, dovodi do poremećaja stvaranja i oslobađanja faktora poreklom iz endotela. Cilj ovog istraživanja bio je da se ispita uticaj okluzije karotidne arterije (10, 30 i 60 min) na vazokontraktilne efekte norepinefrina (NOR) i neuropeptida Y (NPY) na izolovanoj žlezdanoj grani facijalne arterije kunića, glavne dovodne arterije za submandibularnu žlezdu. Metode. U kupatilu za izolovane krvne sudove ispitivane su izometrijske promene tonusa arterijskih preparata, pre i posle okluzije karotidne arterije. Rezultati. Koncentracijski-zavisan vazokontraktilni efekat NOR bio je značajno veći posle 30 i 60 min karotidne okluzije, ali samo na preparatima sa očuvanim endotelom. Primenjen u rastućim koncentracijama, NPY nije imao efekta na tonus izolovane žlezdane grane facijalne aterije kunića, ali je povećao vazokontraktilni efekat NOR na svim ispitivanim preparatima. Povećanje vazokontraktilnog efekta NOR u prisustvu NPY bilo je značajno manje posle 30 i 60 min okluzije. Takođe, efekat NPY posle karotidne okluzije bio je značajno veći na preparatima sa očuvanim endotelom u odnosu na preparate sa uklonjenim endotelom. Zaključak. Ovo istraživanje pokazalo je povećanje vazokontraktilnog efekta NOR i smanjenje potencirajućeg efekta NPY na vazokonstrikciju izazvanu NOR na facijalnoj arteriji kunića posle okluzije karotidne arterije koje su povezane sa promenjenom funkcijom endotela.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Effect of neuropeptide Y on norepinephrine-induced constriction in the rabbit facial artery after carotid artery occlusion
T1  - Efekat neuropeptida Y na konstrikciju facijalne arterije kunića izazvane norepinefrinom posle okluzije karotidne arterije
VL  - 71
IS  - 6
SP  - 571
EP  - 575
DO  - 10.2298/VSP1406571R
ER  - 

@article{
author = "Roganović, Jelena and Petrović, Nina and Đukić, Ljiljana",
year = "2014",
abstract = "Background/Aim. Atherosclerotic-occlusive changes could be observed in orofacial branches of the external carotid artery. Atherosclerosis-induced ischemia caused alteration in production and release of endothelial factors. The aim of this study was to investigate the influence of carotid artery occlusion (10, 30 and 60 min) on vascular effects of norepinephrine (NOR) and neuropeptide Y (NPY) in the isolated glandular branch of the rabbit facial artery, the main feeding artery for the submandibular gland. Method. Changes in isometric tension were recorded in organ bath studies with arterial rings, before and after carotid artery occlusion. Results. Concentrationdependent vasocontractile effect of NOR was significantly augmented after 30 and 60 min of carotid occlusion, but only in the rings with intact endothelium. Given alone, NPY showed no effect in isolated glandular branch of the rabbit facial artery, but enhanced NOR vasoconstriction in all the investigated rings. NOR vasocontractile effect enhancement in the presence of NPY was attenuated after 30 and 60 min of carotid occlusion. Also, enhancement of NOR vasoconstriction by NPY was significantly higher in endothelium-intact rings compared to endotheliumdenuded rings obtained after 30 and 60 min of carotid occlusion. Conclusion. The present investigation provides results of increased vasocontractile effect of NOR and decreased enhancing effect of NPY on NOR vasoconstriction in the rabbit facial artery after carotid occlusion that is related to altered endothelium function., Uvod/Cilj. Pokazano je da se okluzivne promene aterosklerotične prirode mogu opaziti na orofacijalnim granama spoljne karotidne arterije. Ishemija izazvana aterosklerozom, dovodi do poremećaja stvaranja i oslobađanja faktora poreklom iz endotela. Cilj ovog istraživanja bio je da se ispita uticaj okluzije karotidne arterije (10, 30 i 60 min) na vazokontraktilne efekte norepinefrina (NOR) i neuropeptida Y (NPY) na izolovanoj žlezdanoj grani facijalne arterije kunića, glavne dovodne arterije za submandibularnu žlezdu. Metode. U kupatilu za izolovane krvne sudove ispitivane su izometrijske promene tonusa arterijskih preparata, pre i posle okluzije karotidne arterije. Rezultati. Koncentracijski-zavisan vazokontraktilni efekat NOR bio je značajno veći posle 30 i 60 min karotidne okluzije, ali samo na preparatima sa očuvanim endotelom. Primenjen u rastućim koncentracijama, NPY nije imao efekta na tonus izolovane žlezdane grane facijalne aterije kunića, ali je povećao vazokontraktilni efekat NOR na svim ispitivanim preparatima. Povećanje vazokontraktilnog efekta NOR u prisustvu NPY bilo je značajno manje posle 30 i 60 min okluzije. Takođe, efekat NPY posle karotidne okluzije bio je značajno veći na preparatima sa očuvanim endotelom u odnosu na preparate sa uklonjenim endotelom. Zaključak. Ovo istraživanje pokazalo je povećanje vazokontraktilnog efekta NOR i smanjenje potencirajućeg efekta NPY na vazokonstrikciju izazvanu NOR na facijalnoj arteriji kunića posle okluzije karotidne arterije koje su povezane sa promenjenom funkcijom endotela.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Effect of neuropeptide Y on norepinephrine-induced constriction in the rabbit facial artery after carotid artery occlusion, Efekat neuropeptida Y na konstrikciju facijalne arterije kunića izazvane norepinefrinom posle okluzije karotidne arterije",
volume = "71",
number = "6",
pages = "571-575",
doi = "10.2298/VSP1406571R"
}

Roganović, J., Petrović, N.,& Đukić, L.. (2014). Effect of neuropeptide Y on norepinephrine-induced constriction in the rabbit facial artery after carotid artery occlusion. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 71(6), 571-575.
https://doi.org/10.2298/VSP1406571R

Roganović J, Petrović N, Đukić L. Effect of neuropeptide Y on norepinephrine-induced constriction in the rabbit facial artery after carotid artery occlusion. in Vojnosanitetski pregled. 2014;71(6):571-575.
doi:10.2298/VSP1406571R .

Roganović, Jelena, Petrović, Nina, Đukić, Ljiljana, "Effect of neuropeptide Y on norepinephrine-induced constriction in the rabbit facial artery after carotid artery occlusion" in Vojnosanitetski pregled, 71, no. 6 (2014):571-575,
https://doi.org/10.2298/VSP1406571R . .