TY  - JOUR
AU  - Lepojević, Nikola
AU  - Šćepan, Ivana
AU  - Glišić, Branislav
AU  - Jenko, Monika
AU  - Godec, Matjaz
AU  - Hocevar, Samo
AU  - Rudolf, Rebeka
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2375
AB  - Nickel-titanium (NiTi) orthodontic archwires are crucial in the initial stages of orthodontic therapy when the movement of teeth and deflection of the archwire are the largest. Their great mechanical properties come with their main disadvantage-the leakage of nickel. Various in vitro studies measured nickel leakage from archwires that were only immersed in the medium with little or minimal simulation of all stress and deflection forces that affect them. This study aims to overcome that by simulating deflection forces that those archwires are exposed to inside the mouth of a patient. NiTi orthodontic archwires were immersed in CACO2-2 cell culture medium and then immediately loaded while using a simulator of multiaxial stress for 24 h. After the experiment, the surface of the NiTi orthodontic archwires were analysed while using scanning electron microscopy (SEM) and auger electron spectroscopy (AES). The observations showed significant microstructural and compositional changes within the first 51 nm thickness of the archwire surface. Furthermore, the released nickel and titanium concentrations in the CACO2-2 cell culture medium were measured while using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). It was found out that the level of released nickel ions was 1.310 mu g/L, which can be assigned as statistically significant results. These data represent the first mention of the already detectable release of Ni ions after 24 h during the simulation of mechanical loading in the CACO2-2 cell culture medium, which is important for clinical orthodontic praxis.
PB  - Mdpi, Basel
T2  - Coatings
T1  - Characterisation of NiTi Orthodontic Archwires Surface after the Simulation of Mechanical Loading in CACO2-2 Cell Culture
VL  - 9
IS  - 7
DO  - 10.3390/coatings9070440
ER  - 

@article{
author = "Lepojević, Nikola and Šćepan, Ivana and Glišić, Branislav and Jenko, Monika and Godec, Matjaz and Hocevar, Samo and Rudolf, Rebeka",
year = "2019",
abstract = "Nickel-titanium (NiTi) orthodontic archwires are crucial in the initial stages of orthodontic therapy when the movement of teeth and deflection of the archwire are the largest. Their great mechanical properties come with their main disadvantage-the leakage of nickel. Various in vitro studies measured nickel leakage from archwires that were only immersed in the medium with little or minimal simulation of all stress and deflection forces that affect them. This study aims to overcome that by simulating deflection forces that those archwires are exposed to inside the mouth of a patient. NiTi orthodontic archwires were immersed in CACO2-2 cell culture medium and then immediately loaded while using a simulator of multiaxial stress for 24 h. After the experiment, the surface of the NiTi orthodontic archwires were analysed while using scanning electron microscopy (SEM) and auger electron spectroscopy (AES). The observations showed significant microstructural and compositional changes within the first 51 nm thickness of the archwire surface. Furthermore, the released nickel and titanium concentrations in the CACO2-2 cell culture medium were measured while using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). It was found out that the level of released nickel ions was 1.310 mu g/L, which can be assigned as statistically significant results. These data represent the first mention of the already detectable release of Ni ions after 24 h during the simulation of mechanical loading in the CACO2-2 cell culture medium, which is important for clinical orthodontic praxis.",
publisher = "Mdpi, Basel",
journal = "Coatings",
title = "Characterisation of NiTi Orthodontic Archwires Surface after the Simulation of Mechanical Loading in CACO2-2 Cell Culture",
volume = "9",
number = "7",
doi = "10.3390/coatings9070440"
}

Lepojević, N., Šćepan, I., Glišić, B., Jenko, M., Godec, M., Hocevar, S.,& Rudolf, R.. (2019). Characterisation of NiTi Orthodontic Archwires Surface after the Simulation of Mechanical Loading in CACO2-2 Cell Culture. in Coatings
Mdpi, Basel., 9(7).
https://doi.org/10.3390/coatings9070440

Lepojević N, Šćepan I, Glišić B, Jenko M, Godec M, Hocevar S, Rudolf R. Characterisation of NiTi Orthodontic Archwires Surface after the Simulation of Mechanical Loading in CACO2-2 Cell Culture. in Coatings. 2019;9(7).
doi:10.3390/coatings9070440 .

Lepojević, Nikola, Šćepan, Ivana, Glišić, Branislav, Jenko, Monika, Godec, Matjaz, Hocevar, Samo, Rudolf, Rebeka, "Characterisation of NiTi Orthodontic Archwires Surface after the Simulation of Mechanical Loading in CACO2-2 Cell Culture" in Coatings, 9, no. 7 (2019),
https://doi.org/10.3390/coatings9070440 . .