TY  - JOUR
AU  - Krunić, Jelena
AU  - Stojanović, Nikola
AU  - Đukić, Ljiljana
AU  - Roganović, Jelena
AU  - Popović, Branka
AU  - Simić, Ivana
AU  - Stojić, Dragica
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2465
AB  - ObjectivesTo evaluate local effect of gaseous ozone on bacteria in deep carious lesions after incomplete caries removal, using chlorhexidine as control, and to investigate its effect on pulp vascular endothelial growth factor (VEGF), neuronal nitric oxide synthase (nNOS), and superoxide dismutase (SOD).Materials and methodsAntibacterial effect was evaluated in 48 teeth with diagnosed deep carious lesion. After incomplete caries removal, teeth were randomly allocated into two groups regarding the cavity disinfectant used: ozone (open system) or 2% chlorhexidine. Dentin samples were analyzed for the presence of total bacteria and Lactobacillus spp. by real-time quantitative polymerase chain reaction. For evaluation of ozone effect on dental pulp, 38 intact permanent teeth indicated for pulp removal/tooth extraction were included. After cavity preparation, teeth were randomly allocated into two groups: ozone group and control group. VEGF/nNOS level and SOD activity in dental pulp were determined by enzyme-linked immunosorbent assay and spectrophotometric method, respectively.ResultsOzone application decreased number of total bacteria (p=0.001) and Lactobacillus spp. (p lt 0.001), similarly to chlorhexidine. The VEGF (p lt 0.001) and nNOS (p=0.012) levels in dental pulp after ozone application were higher, while SOD activity was lower (p=0.001) comparing to those in control pulp.ConclusionsAntibacterial effect of ozone on residual bacteria after incomplete caries removal was similar to that of 2% chlorhexidine. Effect of ozone on pulp VEGF, nNOS, and SOD indicated its biocompatibility.Clinical relevanceOzone appears as effective and biocompatible cavity disinfectant in treatment of deep carious lesions by incomplete caries removal technique.
PB  - Springer Heidelberg, Heidelberg
T2  - Clinical Oral Investigations
T1  - Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal
VL  - 23
IS  - 2
SP  - 785
EP  - 792
DO  - 10.1007/s00784-018-2495-x
ER  - 

@article{
author = "Krunić, Jelena and Stojanović, Nikola and Đukić, Ljiljana and Roganović, Jelena and Popović, Branka and Simić, Ivana and Stojić, Dragica",
year = "2019",
abstract = "ObjectivesTo evaluate local effect of gaseous ozone on bacteria in deep carious lesions after incomplete caries removal, using chlorhexidine as control, and to investigate its effect on pulp vascular endothelial growth factor (VEGF), neuronal nitric oxide synthase (nNOS), and superoxide dismutase (SOD).Materials and methodsAntibacterial effect was evaluated in 48 teeth with diagnosed deep carious lesion. After incomplete caries removal, teeth were randomly allocated into two groups regarding the cavity disinfectant used: ozone (open system) or 2% chlorhexidine. Dentin samples were analyzed for the presence of total bacteria and Lactobacillus spp. by real-time quantitative polymerase chain reaction. For evaluation of ozone effect on dental pulp, 38 intact permanent teeth indicated for pulp removal/tooth extraction were included. After cavity preparation, teeth were randomly allocated into two groups: ozone group and control group. VEGF/nNOS level and SOD activity in dental pulp were determined by enzyme-linked immunosorbent assay and spectrophotometric method, respectively.ResultsOzone application decreased number of total bacteria (p=0.001) and Lactobacillus spp. (p lt 0.001), similarly to chlorhexidine. The VEGF (p lt 0.001) and nNOS (p=0.012) levels in dental pulp after ozone application were higher, while SOD activity was lower (p=0.001) comparing to those in control pulp.ConclusionsAntibacterial effect of ozone on residual bacteria after incomplete caries removal was similar to that of 2% chlorhexidine. Effect of ozone on pulp VEGF, nNOS, and SOD indicated its biocompatibility.Clinical relevanceOzone appears as effective and biocompatible cavity disinfectant in treatment of deep carious lesions by incomplete caries removal technique.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Clinical Oral Investigations",
title = "Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal",
volume = "23",
number = "2",
pages = "785-792",
doi = "10.1007/s00784-018-2495-x"
}

Krunić, J., Stojanović, N., Đukić, L., Roganović, J., Popović, B., Simić, I.,& Stojić, D.. (2019). Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal. in Clinical Oral Investigations
Springer Heidelberg, Heidelberg., 23(2), 785-792.
https://doi.org/10.1007/s00784-018-2495-x

Krunić J, Stojanović N, Đukić L, Roganović J, Popović B, Simić I, Stojić D. Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal. in Clinical Oral Investigations. 2019;23(2):785-792.
doi:10.1007/s00784-018-2495-x .

Krunić, Jelena, Stojanović, Nikola, Đukić, Ljiljana, Roganović, Jelena, Popović, Branka, Simić, Ivana, Stojić, Dragica, "Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal" in Clinical Oral Investigations, 23, no. 2 (2019):785-792,
https://doi.org/10.1007/s00784-018-2495-x . .

TY  - JOUR
AU  - Vejnović, Dubravka
AU  - Milić, Vera
AU  - Popović, Branka
AU  - Damnjanović, Tatjana
AU  - Maksimović, Nela
AU  - Bunjevački, Vera
AU  - Krajinović, Maja
AU  - Novaković, Ivana
AU  - Damjanov, Nemanja
AU  - Jekić, Biljana
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2526
AB  - Background: Methotrexate (MTX), a folate analogue, is the most commonly used disease-modifying drug in the treatment of rheumatoid arthritis (RA). However, high interindividual differences in drug response are present among RA patients.Research design and methods: In a group of 234 RA patients treated with MTX, we investigated whether rs1650697 polymorphism in DHFR gene may have an impact on MTX efficacy and/or adverse drug effects (ADEs). Relative DAS28 values (rDAS28) were used for the estimation of MTX therapy and all ADEs were recorded. Patients were genotyped for selected polymorphism by real-time PCR method. Results: According to the European League Against Rheumatism criteria after 6 months of MTX therapy, 196 patients (83.8%) were classified as responders (25 (10.7%) were good and 171 (73.1%) were moderate) and 38 patients (16.2%) as nonresponders. ADEs were observed in 55 patients (23.5%). Conclusions: Our results showed that the presence of T allele might be protective against MTX hepatotoxicity measured by transaminase levels (p = 0.05). Furthermore, among patients who also received low-dose corticosteroids, we have found a lower rDAS value in patients with CC genotype (p = 0.039).
PB  - Taylor & Francis Ltd, Abingdon
T2  - Expert Opinion on Drug Metabolism & Toxicology
T1  - Association of C35T polymorphism in dihydrofolate reductase gene with toxicity of methotrexate in rheumatoid arthritis patients
VL  - 15
IS  - 3
SP  - 253
EP  - 257
DO  - 10.1080/17425255.2019.1563594
ER  - 

@article{
author = "Vejnović, Dubravka and Milić, Vera and Popović, Branka and Damnjanović, Tatjana and Maksimović, Nela and Bunjevački, Vera and Krajinović, Maja and Novaković, Ivana and Damjanov, Nemanja and Jekić, Biljana",
year = "2019",
abstract = "Background: Methotrexate (MTX), a folate analogue, is the most commonly used disease-modifying drug in the treatment of rheumatoid arthritis (RA). However, high interindividual differences in drug response are present among RA patients.Research design and methods: In a group of 234 RA patients treated with MTX, we investigated whether rs1650697 polymorphism in DHFR gene may have an impact on MTX efficacy and/or adverse drug effects (ADEs). Relative DAS28 values (rDAS28) were used for the estimation of MTX therapy and all ADEs were recorded. Patients were genotyped for selected polymorphism by real-time PCR method. Results: According to the European League Against Rheumatism criteria after 6 months of MTX therapy, 196 patients (83.8%) were classified as responders (25 (10.7%) were good and 171 (73.1%) were moderate) and 38 patients (16.2%) as nonresponders. ADEs were observed in 55 patients (23.5%). Conclusions: Our results showed that the presence of T allele might be protective against MTX hepatotoxicity measured by transaminase levels (p = 0.05). Furthermore, among patients who also received low-dose corticosteroids, we have found a lower rDAS value in patients with CC genotype (p = 0.039).",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Expert Opinion on Drug Metabolism & Toxicology",
title = "Association of C35T polymorphism in dihydrofolate reductase gene with toxicity of methotrexate in rheumatoid arthritis patients",
volume = "15",
number = "3",
pages = "253-257",
doi = "10.1080/17425255.2019.1563594"
}

Vejnović, D., Milić, V., Popović, B., Damnjanović, T., Maksimović, N., Bunjevački, V., Krajinović, M., Novaković, I., Damjanov, N.,& Jekić, B.. (2019). Association of C35T polymorphism in dihydrofolate reductase gene with toxicity of methotrexate in rheumatoid arthritis patients. in Expert Opinion on Drug Metabolism & Toxicology
Taylor & Francis Ltd, Abingdon., 15(3), 253-257.
https://doi.org/10.1080/17425255.2019.1563594

Vejnović D, Milić V, Popović B, Damnjanović T, Maksimović N, Bunjevački V, Krajinović M, Novaković I, Damjanov N, Jekić B. Association of C35T polymorphism in dihydrofolate reductase gene with toxicity of methotrexate in rheumatoid arthritis patients. in Expert Opinion on Drug Metabolism & Toxicology. 2019;15(3):253-257.
doi:10.1080/17425255.2019.1563594 .

Vejnović, Dubravka, Milić, Vera, Popović, Branka, Damnjanović, Tatjana, Maksimović, Nela, Bunjevački, Vera, Krajinović, Maja, Novaković, Ivana, Damjanov, Nemanja, Jekić, Biljana, "Association of C35T polymorphism in dihydrofolate reductase gene with toxicity of methotrexate in rheumatoid arthritis patients" in Expert Opinion on Drug Metabolism & Toxicology, 15, no. 3 (2019):253-257,
https://doi.org/10.1080/17425255.2019.1563594 . .

TY  - JOUR
AU  - Vasić, Marko
AU  - Loncar-Turukalo, Tatjana
AU  - Tasić, Tatjana
AU  - Matić, Marija
AU  - Glumac, Sofija
AU  - Bajić, Dragana
AU  - Popović, Branka
AU  - Japundžić-Žigon, Nina
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2469
AB  - Using comprehensive analysis of heart rate (HRV) and blood pressure (BPV) short-term variability we estimated the time course of changes of autonomic nervous system remodeling in two stages of doxorubicin-induced cardiomyopathy (DCM). We also investigated the level of gene expression of cardiac beta-1 (beta-1AR) and beta-2 (beta-2AR) adrenoceptors. Experiments were performed in adult male Wistar rats equipped with indwelling catheters for BP recording and blood withdrawal. A 15 mg/kg total cumulative dose of doxorubicin was injected i.p. to rats to induce DCM or saline for control (n = 18). Rats were assessed for general toxicity, cardiovascular hemodynamic and echocardiography before treatment (n = 6), 35 days (DOX35; n = 6) and 70 days (DOX70; n = 6) post-treatment. HRV was evaluated by spectral analysis, Poincare plots, sample and approximate entropy. Expression of beta-1AR and beta-2AR mRNA was evaluated by RT-qPCR. Doxorubicin-treated rats exhibited poor general condition and lower survival than saline-treated rats. In DOX35 rats, there were no echocardiography signs of decompensation, no increase in serum cardiac troponins, but there was an increase of HRV and decrease of HR complexity. In these rats typical microscopic signs of cardiotoxicity were seen along with over-expression of beta-1AR mRNA. 70 days post-treatment echocardiography revealed signs of decompensation and serum cardiac troponin T was increased. At this stage BPV decreased. In conclusion, HRV increase matches transient over expression of cardiac beta-1AR mRNA in compensate stage of DCM while decompensate stage of DCM is characterized by a decrease of BPV and no changes in beta-1AR and beta-2AR gene expression.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Toxicology & Applied Pharmacology
T1  - Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy
VL  - 362
SP  - 43
EP  - 51
DO  - 10.1016/j.taap.2018.10.015
ER  - 

@article{
author = "Vasić, Marko and Loncar-Turukalo, Tatjana and Tasić, Tatjana and Matić, Marija and Glumac, Sofija and Bajić, Dragana and Popović, Branka and Japundžić-Žigon, Nina",
year = "2019",
abstract = "Using comprehensive analysis of heart rate (HRV) and blood pressure (BPV) short-term variability we estimated the time course of changes of autonomic nervous system remodeling in two stages of doxorubicin-induced cardiomyopathy (DCM). We also investigated the level of gene expression of cardiac beta-1 (beta-1AR) and beta-2 (beta-2AR) adrenoceptors. Experiments were performed in adult male Wistar rats equipped with indwelling catheters for BP recording and blood withdrawal. A 15 mg/kg total cumulative dose of doxorubicin was injected i.p. to rats to induce DCM or saline for control (n = 18). Rats were assessed for general toxicity, cardiovascular hemodynamic and echocardiography before treatment (n = 6), 35 days (DOX35; n = 6) and 70 days (DOX70; n = 6) post-treatment. HRV was evaluated by spectral analysis, Poincare plots, sample and approximate entropy. Expression of beta-1AR and beta-2AR mRNA was evaluated by RT-qPCR. Doxorubicin-treated rats exhibited poor general condition and lower survival than saline-treated rats. In DOX35 rats, there were no echocardiography signs of decompensation, no increase in serum cardiac troponins, but there was an increase of HRV and decrease of HR complexity. In these rats typical microscopic signs of cardiotoxicity were seen along with over-expression of beta-1AR mRNA. 70 days post-treatment echocardiography revealed signs of decompensation and serum cardiac troponin T was increased. At this stage BPV decreased. In conclusion, HRV increase matches transient over expression of cardiac beta-1AR mRNA in compensate stage of DCM while decompensate stage of DCM is characterized by a decrease of BPV and no changes in beta-1AR and beta-2AR gene expression.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Toxicology & Applied Pharmacology",
title = "Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy",
volume = "362",
pages = "43-51",
doi = "10.1016/j.taap.2018.10.015"
}

Vasić, M., Loncar-Turukalo, T., Tasić, T., Matić, M., Glumac, S., Bajić, D., Popović, B.,& Japundžić-Žigon, N.. (2019). Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy. in Toxicology & Applied Pharmacology
Academic Press Inc Elsevier Science, San Diego., 362, 43-51.
https://doi.org/10.1016/j.taap.2018.10.015

Vasić M, Loncar-Turukalo T, Tasić T, Matić M, Glumac S, Bajić D, Popović B, Japundžić-Žigon N. Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy. in Toxicology & Applied Pharmacology. 2019;362:43-51.
doi:10.1016/j.taap.2018.10.015 .

Vasić, Marko, Loncar-Turukalo, Tatjana, Tasić, Tatjana, Matić, Marija, Glumac, Sofija, Bajić, Dragana, Popović, Branka, Japundžić-Žigon, Nina, "Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy" in Toxicology & Applied Pharmacology, 362 (2019):43-51,
https://doi.org/10.1016/j.taap.2018.10.015 . .

TY  - JOUR
AU  - Vejnović, Dubravka
AU  - Milić, Vera
AU  - Popović, Branka
AU  - Damnjanović, Tatjana
AU  - Maksimović, Nela
AU  - Bunjevački, Vera
AU  - Krajinović, Maja
AU  - Novaković, Ivana
AU  - Damjanov, Nemanja
AU  - Jekić, Biljana
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2462
AB  - Background: Methotrexate (MTX), a folate analogue, is the most commonly used disease-modifying drug in the treatment of rheumatoid arthritis (RA). However, high interindividual differences in drug response are present among RA patients.Research design and methods: In a group of 234 RA patients treated with MTX, we investigated whether rs1650697 polymorphism in DHFR gene may have an impact on MTX efficacy and/or adverse drug effects (ADEs). Relative DAS28 values (rDAS28) were used for the estimation of MTX therapy and all ADEs were recorded. Patients were genotyped for selected polymorphism by real-time PCR method. Results: According to the European League Against Rheumatism criteria after 6 months of MTX therapy, 196 patients (83.8%) were classified as responders (25 (10.7%) were good and 171 (73.1%) were moderate) and 38 patients (16.2%) as nonresponders. ADEs were observed in 55 patients (23.5%). Conclusions: Our results showed that the presence of T allele might be protective against MTX hepatotoxicity measured by transaminase levels (p = 0.05). Furthermore, among patients who also received low-dose corticosteroids, we have found a lower rDAS value in patients with CC genotype (p = 0.039).
PB  - Taylor & Francis Ltd, Abingdon
T2  - Expert Opinion on Drug Metabolism & Toxicology
T1  - Association of C35T polymorphism in dihydrofolate reductase gene with toxicity of methotrexate in rheumatoid arthritis patients
VL  - 15
IS  - 3
SP  - 253
EP  - 257
DO  - 10.1080/17425255.2019.1563594
ER  - 

@article{
author = "Vejnović, Dubravka and Milić, Vera and Popović, Branka and Damnjanović, Tatjana and Maksimović, Nela and Bunjevački, Vera and Krajinović, Maja and Novaković, Ivana and Damjanov, Nemanja and Jekić, Biljana",
year = "2019",
abstract = "Background: Methotrexate (MTX), a folate analogue, is the most commonly used disease-modifying drug in the treatment of rheumatoid arthritis (RA). However, high interindividual differences in drug response are present among RA patients.Research design and methods: In a group of 234 RA patients treated with MTX, we investigated whether rs1650697 polymorphism in DHFR gene may have an impact on MTX efficacy and/or adverse drug effects (ADEs). Relative DAS28 values (rDAS28) were used for the estimation of MTX therapy and all ADEs were recorded. Patients were genotyped for selected polymorphism by real-time PCR method. Results: According to the European League Against Rheumatism criteria after 6 months of MTX therapy, 196 patients (83.8%) were classified as responders (25 (10.7%) were good and 171 (73.1%) were moderate) and 38 patients (16.2%) as nonresponders. ADEs were observed in 55 patients (23.5%). Conclusions: Our results showed that the presence of T allele might be protective against MTX hepatotoxicity measured by transaminase levels (p = 0.05). Furthermore, among patients who also received low-dose corticosteroids, we have found a lower rDAS value in patients with CC genotype (p = 0.039).",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Expert Opinion on Drug Metabolism & Toxicology",
title = "Association of C35T polymorphism in dihydrofolate reductase gene with toxicity of methotrexate in rheumatoid arthritis patients",
volume = "15",
number = "3",
pages = "253-257",
doi = "10.1080/17425255.2019.1563594"
}

Vejnović, D., Milić, V., Popović, B., Damnjanović, T., Maksimović, N., Bunjevački, V., Krajinović, M., Novaković, I., Damjanov, N.,& Jekić, B.. (2019). Association of C35T polymorphism in dihydrofolate reductase gene with toxicity of methotrexate in rheumatoid arthritis patients. in Expert Opinion on Drug Metabolism & Toxicology
Taylor & Francis Ltd, Abingdon., 15(3), 253-257.
https://doi.org/10.1080/17425255.2019.1563594

Vejnović D, Milić V, Popović B, Damnjanović T, Maksimović N, Bunjevački V, Krajinović M, Novaković I, Damjanov N, Jekić B. Association of C35T polymorphism in dihydrofolate reductase gene with toxicity of methotrexate in rheumatoid arthritis patients. in Expert Opinion on Drug Metabolism & Toxicology. 2019;15(3):253-257.
doi:10.1080/17425255.2019.1563594 .

Vejnović, Dubravka, Milić, Vera, Popović, Branka, Damnjanović, Tatjana, Maksimović, Nela, Bunjevački, Vera, Krajinović, Maja, Novaković, Ivana, Damjanov, Nemanja, Jekić, Biljana, "Association of C35T polymorphism in dihydrofolate reductase gene with toxicity of methotrexate in rheumatoid arthritis patients" in Expert Opinion on Drug Metabolism & Toxicology, 15, no. 3 (2019):253-257,
https://doi.org/10.1080/17425255.2019.1563594 . .

TY  - JOUR
AU  - Popović, Branka
AU  - Velimirović, Milica
AU  - Stojković, Tihomir
AU  - Brajović, Gavrilo
AU  - de Luka, Silvio R.
AU  - Milovanović, Ivan
AU  - Stefanović, Srđan
AU  - Nikolić, Dragica
AU  - Ristic-Đurović, Jasna L.
AU  - Petronijević, Nataša
AU  - Trbovich, Alexander M.
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2323
AB  - Ageing affects various physiological and metabolic processes in a body and a progressive accumulation of oxidative damage stands out as often used explanation. One of the most powerful scavenger of reactive oxygen species (ROS) in all organs is melatonin. A majority of melatonin supplied to the body via blood originates from the pineal gland. However, we have been interested in a locally produced melatonin. We have used 2.5- and 36-months-old Wistar rats. Tissues were collected and gene expression of AA-NAT and ASMT, the two key enzymes in a synthesis of melatonin, was determined in brain, liver, kidney, heart, skin, and intestine. Since melatonin can influence antioxidant enzymes, the activity of superoxide dismutase (SOD) and catalase (CAT), and the level of GSH were measured in liver. In addition, Copper (Cu), Zinc (Zn), and Manganese (Mn) were also determined in liver since these microelements might affect the activity of antioxidant enzymes. The expression of AA-NAT and ASMT was increased in liver and skin of old animals. A positive correlation in AA-NAT and ASMT expression was observed in liver, intestine and kidney. Moreover, the activity of CAT enzyme in liver was increased while SOD activity was decreased. SOD and CAT were probably affected by the observed decreased amount of Cu, Zn, and Mn in liver of old animals. In our model, extrapineal melatonin pathway in ageing consisted of complex interplay of locally produced melatonin, activities of SOD and CAT, and adequate presence of Cu, Zn and Mn microelements in order to defend organs against oxidative damage.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - The influence of ageing on the extrapineal melatonin synthetic pathway
VL  - 110
SP  - 151
EP  - 157
DO  - 10.1016/j.exger.2018.06.010
ER  - 

@article{
author = "Popović, Branka and Velimirović, Milica and Stojković, Tihomir and Brajović, Gavrilo and de Luka, Silvio R. and Milovanović, Ivan and Stefanović, Srđan and Nikolić, Dragica and Ristic-Đurović, Jasna L. and Petronijević, Nataša and Trbovich, Alexander M.",
year = "2018",
abstract = "Ageing affects various physiological and metabolic processes in a body and a progressive accumulation of oxidative damage stands out as often used explanation. One of the most powerful scavenger of reactive oxygen species (ROS) in all organs is melatonin. A majority of melatonin supplied to the body via blood originates from the pineal gland. However, we have been interested in a locally produced melatonin. We have used 2.5- and 36-months-old Wistar rats. Tissues were collected and gene expression of AA-NAT and ASMT, the two key enzymes in a synthesis of melatonin, was determined in brain, liver, kidney, heart, skin, and intestine. Since melatonin can influence antioxidant enzymes, the activity of superoxide dismutase (SOD) and catalase (CAT), and the level of GSH were measured in liver. In addition, Copper (Cu), Zinc (Zn), and Manganese (Mn) were also determined in liver since these microelements might affect the activity of antioxidant enzymes. The expression of AA-NAT and ASMT was increased in liver and skin of old animals. A positive correlation in AA-NAT and ASMT expression was observed in liver, intestine and kidney. Moreover, the activity of CAT enzyme in liver was increased while SOD activity was decreased. SOD and CAT were probably affected by the observed decreased amount of Cu, Zn, and Mn in liver of old animals. In our model, extrapineal melatonin pathway in ageing consisted of complex interplay of locally produced melatonin, activities of SOD and CAT, and adequate presence of Cu, Zn and Mn microelements in order to defend organs against oxidative damage.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "The influence of ageing on the extrapineal melatonin synthetic pathway",
volume = "110",
pages = "151-157",
doi = "10.1016/j.exger.2018.06.010"
}

Popović, B., Velimirović, M., Stojković, T., Brajović, G., de Luka, S. R., Milovanović, I., Stefanović, S., Nikolić, D., Ristic-Đurović, J. L., Petronijević, N.,& Trbovich, A. M.. (2018). The influence of ageing on the extrapineal melatonin synthetic pathway. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 110, 151-157.
https://doi.org/10.1016/j.exger.2018.06.010

Popović B, Velimirović M, Stojković T, Brajović G, de Luka SR, Milovanović I, Stefanović S, Nikolić D, Ristic-Đurović JL, Petronijević N, Trbovich AM. The influence of ageing on the extrapineal melatonin synthetic pathway. in Experimental Gerontology. 2018;110:151-157.
doi:10.1016/j.exger.2018.06.010 .

Popović, Branka, Velimirović, Milica, Stojković, Tihomir, Brajović, Gavrilo, de Luka, Silvio R., Milovanović, Ivan, Stefanović, Srđan, Nikolić, Dragica, Ristic-Đurović, Jasna L., Petronijević, Nataša, Trbovich, Alexander M., "The influence of ageing on the extrapineal melatonin synthetic pathway" in Experimental Gerontology, 110 (2018):151-157,
https://doi.org/10.1016/j.exger.2018.06.010 . .

TY  - JOUR
AU  - Nešković, Jelena
AU  - Jovanović-Medojević, Milica
AU  - Grga, Đurica
AU  - Popović, Branka
AU  - Živković, Slavoljub
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2360
AB  - Introduction the main objective of endodontic treatment is to eliminate infection from root canal and prevent re-infection by three-dimensional hermetic obturation of the canal system. Endodontic failure can occur due to inability of complete control and elimination of infection from the root canal. The aim of this study is to investigate, by PCR technique, microbiological status of previously endodontically unsuccessfully treated teeth immediately after the removal of obturation material. Material and Methods the analysis included 30 teeth indicated for endodontic retreatment. After removing previous root canal filling material, the bacteriological sample was taken by sterile instrument (# 15) and paper points. Standard PCR technique was used to analyze the incidence of E.faecalis, P.micros, P.intermedia, P. endodontalis and a.actinomycetemcomitans. Results Positive bacteriological findings were registered in 80% of cases, while bacteria were not identified in 20% of all samples (all taken from the root canals without significant changes in periapical tissue). From 24 canals with identified bacteria, 17 had affected apical periodontium. The most dominant microbe in root canals with positive bacteriological finding was E.faecalis (83.3% of the canals) and P.intermedia (75%). In case of teeth with chronic periapical changes, the most common was E. faecalis (94%) and P.intermedia (82.3%). Conclusion the presence of periapical lesions significantly affects microbiological status of endodontically treated teeth. The presence of bacteria was confirmed in most teeth with periapical lesions, while the most frequently identified bacteria were E. faecalis, P.intermedia and P.micros.
AB  - Uvod Osnovni zadatak endodontskog tretmana je da eliminiše infekciju iz kanala korena I spreči reinfekciju trodimenzionalnom hermetičkom opturacijom kanalskog sistema. Usled nemogućnosti potpune kontrole I eliminacije infekcije iz kanala korena može doći do pojave endodontskog neuspeha. Cilj ovog rada je bio da se neposredno posle uklanjanja materijala za opturaciju kod zuba sa neuspelim endodontskim lečenjem PCR tehnikom proveri mikrobiološki status endodontski lečenih zuba. Metode Mikrobiološka studija je obuhvatila 30 zuba (osam višekorenih I 22 jednokorena) indikovanih za ponovljeni endodontski tretman. Posle dezinfekcije radnog polja I dezopturacije koja je urađena isključivo mehaničkim putem, uzorak je uzet sterilnim kanalnim instrumentom tipa pulpekstirpatora (#15) ili Hoedstrem-turpije (#15) I uz pomoć papirnih poena kojima je sušen kanal. Ependorfice su čuvane na temperaturi od -20˚C do mikrobiološke analize. Putem klasičnog PCR analizirana je zastupljenost bakterija: Enterococcus faecalis, Peptostreptococcus micros, Prevotela intermedia, Porphyromonas endodontalis I Actinobacilus actinomicetemcomitans. Rezultati Pozitivan bakteriološki nalaz registrovan je u 80% slučajeva, dok bakterije nisu identifikovane u 20% uzoraka. Svi negativni uzorci su uzeti iz kanala korena zuba bez značajnih promena u apeksnom periodoncijumu, dok je 17 od 24 kanala sa identifikovanim bakterijama pripadalo zubima sa oštećenim apeksnim parodoncijumom. Najprisutniji mikroorganizam u kanalima sa pozitivnim bakteriološkim nalazom bio je E. faecalis, koji je identifikovan u 83,3% kanala, zatim P. inermedia sa 75% I P. micros sa zastupljenošću od 58,3%. Kod zuba sa hroničnim periapikalnim promenama najzastupljeniji je bio E. faecalis, koji je identifikovan u 94% kanala, zatim P. intermedia, koja je bila prisutna u 82,3% uzoraka. Mikroorganizmi E. faecalis I P. intermedia su registrovani kod svih pacijenata sa nekim od simptoma. Zaključak Prisustvo periapikalnih lezija značajno utiče na mikrobiološki status kanala korena endodontski lečenih zuba. Prisustvo bakterija u kanalima korena potvrđeno je u većini endodontski neuspešno lečenih zuba, a najčećše identifikovane bakterije bile su E. faecalis, zatim P. intermedia, P. micros I P. endodontalis.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Microbiological status of root canal after unsuccessful endodontic treatment
T1  - Mikrobiloški status kanala korena endodonski neuspešno lečenih zuba
VL  - 65
IS  - 4
SP  - 195
EP  - 204
UR  - https://hdl.handle.net/21.15107/rcub_smile_2360
ER  - 

@article{
author = "Nešković, Jelena and Jovanović-Medojević, Milica and Grga, Đurica and Popović, Branka and Živković, Slavoljub",
year = "2018",
abstract = "Introduction the main objective of endodontic treatment is to eliminate infection from root canal and prevent re-infection by three-dimensional hermetic obturation of the canal system. Endodontic failure can occur due to inability of complete control and elimination of infection from the root canal. The aim of this study is to investigate, by PCR technique, microbiological status of previously endodontically unsuccessfully treated teeth immediately after the removal of obturation material. Material and Methods the analysis included 30 teeth indicated for endodontic retreatment. After removing previous root canal filling material, the bacteriological sample was taken by sterile instrument (# 15) and paper points. Standard PCR technique was used to analyze the incidence of E.faecalis, P.micros, P.intermedia, P. endodontalis and a.actinomycetemcomitans. Results Positive bacteriological findings were registered in 80% of cases, while bacteria were not identified in 20% of all samples (all taken from the root canals without significant changes in periapical tissue). From 24 canals with identified bacteria, 17 had affected apical periodontium. The most dominant microbe in root canals with positive bacteriological finding was E.faecalis (83.3% of the canals) and P.intermedia (75%). In case of teeth with chronic periapical changes, the most common was E. faecalis (94%) and P.intermedia (82.3%). Conclusion the presence of periapical lesions significantly affects microbiological status of endodontically treated teeth. The presence of bacteria was confirmed in most teeth with periapical lesions, while the most frequently identified bacteria were E. faecalis, P.intermedia and P.micros., Uvod Osnovni zadatak endodontskog tretmana je da eliminiše infekciju iz kanala korena I spreči reinfekciju trodimenzionalnom hermetičkom opturacijom kanalskog sistema. Usled nemogućnosti potpune kontrole I eliminacije infekcije iz kanala korena može doći do pojave endodontskog neuspeha. Cilj ovog rada je bio da se neposredno posle uklanjanja materijala za opturaciju kod zuba sa neuspelim endodontskim lečenjem PCR tehnikom proveri mikrobiološki status endodontski lečenih zuba. Metode Mikrobiološka studija je obuhvatila 30 zuba (osam višekorenih I 22 jednokorena) indikovanih za ponovljeni endodontski tretman. Posle dezinfekcije radnog polja I dezopturacije koja je urađena isključivo mehaničkim putem, uzorak je uzet sterilnim kanalnim instrumentom tipa pulpekstirpatora (#15) ili Hoedstrem-turpije (#15) I uz pomoć papirnih poena kojima je sušen kanal. Ependorfice su čuvane na temperaturi od -20˚C do mikrobiološke analize. Putem klasičnog PCR analizirana je zastupljenost bakterija: Enterococcus faecalis, Peptostreptococcus micros, Prevotela intermedia, Porphyromonas endodontalis I Actinobacilus actinomicetemcomitans. Rezultati Pozitivan bakteriološki nalaz registrovan je u 80% slučajeva, dok bakterije nisu identifikovane u 20% uzoraka. Svi negativni uzorci su uzeti iz kanala korena zuba bez značajnih promena u apeksnom periodoncijumu, dok je 17 od 24 kanala sa identifikovanim bakterijama pripadalo zubima sa oštećenim apeksnim parodoncijumom. Najprisutniji mikroorganizam u kanalima sa pozitivnim bakteriološkim nalazom bio je E. faecalis, koji je identifikovan u 83,3% kanala, zatim P. inermedia sa 75% I P. micros sa zastupljenošću od 58,3%. Kod zuba sa hroničnim periapikalnim promenama najzastupljeniji je bio E. faecalis, koji je identifikovan u 94% kanala, zatim P. intermedia, koja je bila prisutna u 82,3% uzoraka. Mikroorganizmi E. faecalis I P. intermedia su registrovani kod svih pacijenata sa nekim od simptoma. Zaključak Prisustvo periapikalnih lezija značajno utiče na mikrobiološki status kanala korena endodontski lečenih zuba. Prisustvo bakterija u kanalima korena potvrđeno je u većini endodontski neuspešno lečenih zuba, a najčećše identifikovane bakterije bile su E. faecalis, zatim P. intermedia, P. micros I P. endodontalis.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Microbiological status of root canal after unsuccessful endodontic treatment, Mikrobiloški status kanala korena endodonski neuspešno lečenih zuba",
volume = "65",
number = "4",
pages = "195-204",
url = "https://hdl.handle.net/21.15107/rcub_smile_2360"
}

Nešković, J., Jovanović-Medojević, M., Grga, Đ., Popović, B.,& Živković, S.. (2018). Microbiological status of root canal after unsuccessful endodontic treatment. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 65(4), 195-204.
https://hdl.handle.net/21.15107/rcub_smile_2360

Nešković J, Jovanović-Medojević M, Grga Đ, Popović B, Živković S. Microbiological status of root canal after unsuccessful endodontic treatment. in Stomatološki glasnik Srbije. 2018;65(4):195-204.
https://hdl.handle.net/21.15107/rcub_smile_2360 .

Nešković, Jelena, Jovanović-Medojević, Milica, Grga, Đurica, Popović, Branka, Živković, Slavoljub, "Microbiological status of root canal after unsuccessful endodontic treatment" in Stomatološki glasnik Srbije, 65, no. 4 (2018):195-204,
https://hdl.handle.net/21.15107/rcub_smile_2360 .

TY  - JOUR
AU  - Živković-Sandić, Marija
AU  - Stefanović, Neda
AU  - Popović, Branka
AU  - Glišić, Branislav
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2370
AB  - Tooth agenesis is common dentofacial malformation in humans. Its etiology is still not clear. Hypodontia has been regarded as a multifactorial condition influenced by gene function, environmental interaction and developmental timing. More than 300 genes have been related with patterning, morphogenesis and cell differentiation in teeth. According to data WNT10A gene is considered to have an important role in odontogonesis. The aim of this study was to show mutation status in WNT10A gene in a family with two members with diagnosis of hypodontia/oligodontia. In the reported family (father, mother, son, daughter) children were diagnosed with congenital tooth agenesis (son-2 teeth, daughter-11 teeth), while parents negated congenital absence of teeth. We identified a heterozygous missense mutation, c.682T>A (p.Phe228Ile) within the exon 3 of WNT10A in mother and father and the same homozygous mutation was detected in the same region of WNT10A gene in daughter and son. Observed differences in our study, from no symptoms to mild/severe hypodontia, could be the consequence of genetic influence of c.682T>A(p.Phe228Ile) mutation, but also the contribution of many environmental factors during odontogenesis.
AB  - Urođeni nedostatak zuba predstavlja jednu od najčešćih dentofacijalnih anomalija kod čoveka. Etiologija hipodoncije i dalje nije potpuno definisana i smatra se da su za njenu etiologiju odgovorni brojni genetski i sredinski faktori koji deluju u različitim fazama razvoja zuba. Preko 300 gena povezuje se sa morfogenezom i ćelijskom diferencijacijom u toku razvoja zuba, a prema podacima WNT10A gen je jedan od gena koji ima veoma bitnu ulogu u kontroli odontogeneze. Cilj ovog rada bio je da se prikaže mutacioni status WNT10A gena u porodici sa dijagnostikovanom hipodoncijom/oligodoncijom. U prikazanoj porodici (otac, majka, sin i ćerka) kod dva člana dijagnostikovan je urođeni nedostatak zuba (sin - dva zuba, ćerka - 11 zuba), dok kod roditelja ovaj nedostatak nije zabeležen. Kod svih članova porodice, u okviru egzona 3 WNT10A gena detektovana je mutacija c.682T>A (p.Phe228Ile). Kod majke i oca ova 'missense' mutacija je bila u heterozigotnom obliku, dok je kod sina i ćerke utvrđeno prisustvo iste mutacije u homozigotnom obliku. Zabeležene razlike u analiziranoj porodici, od odsustva simptoma do blage hipodoncije i izrazite oligodoncije, mogu biti posledica prisustva c.682T>A (p.Phe228Ile) mutacije, ali takođe i uticaja faktora sredine u toku odontogeneze.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Hypodontia and WNT10A mutation: A case report
T1  - Hipodoncija i mutacija WNT10A gena - prikaz slučaja
VL  - 65
IS  - 1
SP  - 32
EP  - 36
DO  - 10.2478/sdj-2018-0004
ER  - 

@article{
author = "Živković-Sandić, Marija and Stefanović, Neda and Popović, Branka and Glišić, Branislav",
year = "2018",
abstract = "Tooth agenesis is common dentofacial malformation in humans. Its etiology is still not clear. Hypodontia has been regarded as a multifactorial condition influenced by gene function, environmental interaction and developmental timing. More than 300 genes have been related with patterning, morphogenesis and cell differentiation in teeth. According to data WNT10A gene is considered to have an important role in odontogonesis. The aim of this study was to show mutation status in WNT10A gene in a family with two members with diagnosis of hypodontia/oligodontia. In the reported family (father, mother, son, daughter) children were diagnosed with congenital tooth agenesis (son-2 teeth, daughter-11 teeth), while parents negated congenital absence of teeth. We identified a heterozygous missense mutation, c.682T>A (p.Phe228Ile) within the exon 3 of WNT10A in mother and father and the same homozygous mutation was detected in the same region of WNT10A gene in daughter and son. Observed differences in our study, from no symptoms to mild/severe hypodontia, could be the consequence of genetic influence of c.682T>A(p.Phe228Ile) mutation, but also the contribution of many environmental factors during odontogenesis., Urođeni nedostatak zuba predstavlja jednu od najčešćih dentofacijalnih anomalija kod čoveka. Etiologija hipodoncije i dalje nije potpuno definisana i smatra se da su za njenu etiologiju odgovorni brojni genetski i sredinski faktori koji deluju u različitim fazama razvoja zuba. Preko 300 gena povezuje se sa morfogenezom i ćelijskom diferencijacijom u toku razvoja zuba, a prema podacima WNT10A gen je jedan od gena koji ima veoma bitnu ulogu u kontroli odontogeneze. Cilj ovog rada bio je da se prikaže mutacioni status WNT10A gena u porodici sa dijagnostikovanom hipodoncijom/oligodoncijom. U prikazanoj porodici (otac, majka, sin i ćerka) kod dva člana dijagnostikovan je urođeni nedostatak zuba (sin - dva zuba, ćerka - 11 zuba), dok kod roditelja ovaj nedostatak nije zabeležen. Kod svih članova porodice, u okviru egzona 3 WNT10A gena detektovana je mutacija c.682T>A (p.Phe228Ile). Kod majke i oca ova 'missense' mutacija je bila u heterozigotnom obliku, dok je kod sina i ćerke utvrđeno prisustvo iste mutacije u homozigotnom obliku. Zabeležene razlike u analiziranoj porodici, od odsustva simptoma do blage hipodoncije i izrazite oligodoncije, mogu biti posledica prisustva c.682T>A (p.Phe228Ile) mutacije, ali takođe i uticaja faktora sredine u toku odontogeneze.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Hypodontia and WNT10A mutation: A case report, Hipodoncija i mutacija WNT10A gena - prikaz slučaja",
volume = "65",
number = "1",
pages = "32-36",
doi = "10.2478/sdj-2018-0004"
}

Živković-Sandić, M., Stefanović, N., Popović, B.,& Glišić, B.. (2018). Hypodontia and WNT10A mutation: A case report. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 65(1), 32-36.
https://doi.org/10.2478/sdj-2018-0004

Živković-Sandić M, Stefanović N, Popović B, Glišić B. Hypodontia and WNT10A mutation: A case report. in Stomatološki glasnik Srbije. 2018;65(1):32-36.
doi:10.2478/sdj-2018-0004 .

Živković-Sandić, Marija, Stefanović, Neda, Popović, Branka, Glišić, Branislav, "Hypodontia and WNT10A mutation: A case report" in Stomatološki glasnik Srbije, 65, no. 1 (2018):32-36,
https://doi.org/10.2478/sdj-2018-0004 . .

TY  - JOUR
AU  - Trišić, Dijana
AU  - Ćetenović, Bojana
AU  - Jovanović, Igor
AU  - Gjorgievska, Elizabeta
AU  - Popović, Branka
AU  - Marković, Dejan
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2248
AB  - Background/Aim: The aim of this in vitro study was to investigate the influence of irradiation cycles and resting periods, on thermal effects on the external root surface during root canal irradiation of two diode laser systems (940 nm and 975 nm), at output powers of 1 W and 2 W in continuous mode. In previous studies the rising of temperature above 7°C has been reported as biologically accepted to avoid periodontal damage on the external root surface. Material and Methods: Twenty human inferior incisors were randomly distributed into four groups, the 940 nm, and the 975 nm diode laser irradiation, both with an output power of 1 W and 2 W, in continuous mode. The thermographic camera was used to detect temperature variations on the external root surface. Digital radiography of the samples was made. Results: After three cycles of irradiation, at apical third of the root, mean temperature variation by 940 nm diode laser irradiation was 2.88°C for output power of 1 W, and 6.52°C for output power of 2 W. The 975 nm laser caused a higher temperature increase in the apical region, with temperature variation of 13.56°C by an output power of 1 W, and 30.60°C at 2 W, with a statistical significance of p ≤ 0.0001 between two laser systems compared for the same power. The resting periods of 20 s between cycles were enough to lower temperature under 7°C in the case of 1 W and 2 W for 940 nm diode laser, while for 975 nm laser, after three irradiation cycles overheating occurred at both output power rates. Conclusion: Three cycles irradiation of 940 nm diode laser, with resting periods of 20 seconds, allowed safe usage of 1 W and 2 W in CW for endodontic treatment. For 975 nm at a power rate of 1 W, the last resting period drop the temperature near the safe limit and it came under 7°C in a period less than a minute, while at the power of 2 W the resting periods were not long enough for the safe temperature decrease.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Dental Medicine
T1  - Diode laser irradiation in endodontic therapy through cycles: In vitro study
VL  - 21
IS  - 2
SP  - 108
EP  - 111
DO  - 10.1515/bjdm-2017-0016
ER  - 

@article{
author = "Trišić, Dijana and Ćetenović, Bojana and Jovanović, Igor and Gjorgievska, Elizabeta and Popović, Branka and Marković, Dejan",
year = "2017",
abstract = "Background/Aim: The aim of this in vitro study was to investigate the influence of irradiation cycles and resting periods, on thermal effects on the external root surface during root canal irradiation of two diode laser systems (940 nm and 975 nm), at output powers of 1 W and 2 W in continuous mode. In previous studies the rising of temperature above 7°C has been reported as biologically accepted to avoid periodontal damage on the external root surface. Material and Methods: Twenty human inferior incisors were randomly distributed into four groups, the 940 nm, and the 975 nm diode laser irradiation, both with an output power of 1 W and 2 W, in continuous mode. The thermographic camera was used to detect temperature variations on the external root surface. Digital radiography of the samples was made. Results: After three cycles of irradiation, at apical third of the root, mean temperature variation by 940 nm diode laser irradiation was 2.88°C for output power of 1 W, and 6.52°C for output power of 2 W. The 975 nm laser caused a higher temperature increase in the apical region, with temperature variation of 13.56°C by an output power of 1 W, and 30.60°C at 2 W, with a statistical significance of p ≤ 0.0001 between two laser systems compared for the same power. The resting periods of 20 s between cycles were enough to lower temperature under 7°C in the case of 1 W and 2 W for 940 nm diode laser, while for 975 nm laser, after three irradiation cycles overheating occurred at both output power rates. Conclusion: Three cycles irradiation of 940 nm diode laser, with resting periods of 20 seconds, allowed safe usage of 1 W and 2 W in CW for endodontic treatment. For 975 nm at a power rate of 1 W, the last resting period drop the temperature near the safe limit and it came under 7°C in a period less than a minute, while at the power of 2 W the resting periods were not long enough for the safe temperature decrease.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Dental Medicine",
title = "Diode laser irradiation in endodontic therapy through cycles: In vitro study",
volume = "21",
number = "2",
pages = "108-111",
doi = "10.1515/bjdm-2017-0016"
}

Trišić, D., Ćetenović, B., Jovanović, I., Gjorgievska, E., Popović, B.,& Marković, D.. (2017). Diode laser irradiation in endodontic therapy through cycles: In vitro study. in Balkan Journal of Dental Medicine
Udruženje stomatologa Balkana., 21(2), 108-111.
https://doi.org/10.1515/bjdm-2017-0016

Trišić D, Ćetenović B, Jovanović I, Gjorgievska E, Popović B, Marković D. Diode laser irradiation in endodontic therapy through cycles: In vitro study. in Balkan Journal of Dental Medicine. 2017;21(2):108-111.
doi:10.1515/bjdm-2017-0016 .

Trišić, Dijana, Ćetenović, Bojana, Jovanović, Igor, Gjorgievska, Elizabeta, Popović, Branka, Marković, Dejan, "Diode laser irradiation in endodontic therapy through cycles: In vitro study" in Balkan Journal of Dental Medicine, 21, no. 2 (2017):108-111,
https://doi.org/10.1515/bjdm-2017-0016 . .

TY  - JOUR
AU  - Jelovac, Drago
AU  - Tepavčević, Zvezdana
AU  - Nikolić, Nadja
AU  - Ilić, Branislav
AU  - Eljabo, Najib
AU  - Popović, Branka
AU  - Čarkić, Jelena
AU  - Konstantinović, Vitomir
AU  - Vukadinović, Miroslav
AU  - Miličić, Biljana
AU  - Milašin, Jelena
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2180
AB  - The tumour subtype, TNM classification, and histopathological data are sometimes not sufficient for understanding and assessing the behaviour of oral cancers. In an attempt to find additional markers of tumour biology and behaviour, this study sought to determine the incidence and consequently the relevance of c-erb-B2, c-Myc, and H-ras gene alterations in tumour-free margins of oral squamous cell carcinoma (OSCC). Fifty samples of OSCC were analyzed for c-erb-B2 and c-Myc amplification by real-time polymerase chain reaction and for H-ras point mutations by sequencing. A relatively high incidence of genetic lesions was detected: 22% of cases had c-erb-B2 and 30% had c-Myc amplification, whilst only 12% harboured H-ras mutations. Kaplan-Meier analysis and the log-rank test showed statistically significant differences in 5-year survival rates and relapse between patients with tumour margins positive for c-erb-B2 amplification and those with margins that were negative (P = 0.002). H-ras and c-Myc alterations could not be associated with tumour behaviour. Molecular analysis of margins, targeting cancer genes, could identify additional, independent predictors of risk and outcome in OSCC.
PB  - Churchill Livingstone, Edinburgh
T2  - International Journal of Oral & Maxillofacial Surgery
T1  - The amplification of c-erb-B2 in cancer-free surgical margins is a predictor of poor outcome in oral squamous cell carcinoma
VL  - 45
IS  - 6
SP  - 700
EP  - 705
DO  - 10.1016/j.ijom.2015.11.014
ER  - 

@article{
author = "Jelovac, Drago and Tepavčević, Zvezdana and Nikolić, Nadja and Ilić, Branislav and Eljabo, Najib and Popović, Branka and Čarkić, Jelena and Konstantinović, Vitomir and Vukadinović, Miroslav and Miličić, Biljana and Milašin, Jelena",
year = "2016",
abstract = "The tumour subtype, TNM classification, and histopathological data are sometimes not sufficient for understanding and assessing the behaviour of oral cancers. In an attempt to find additional markers of tumour biology and behaviour, this study sought to determine the incidence and consequently the relevance of c-erb-B2, c-Myc, and H-ras gene alterations in tumour-free margins of oral squamous cell carcinoma (OSCC). Fifty samples of OSCC were analyzed for c-erb-B2 and c-Myc amplification by real-time polymerase chain reaction and for H-ras point mutations by sequencing. A relatively high incidence of genetic lesions was detected: 22% of cases had c-erb-B2 and 30% had c-Myc amplification, whilst only 12% harboured H-ras mutations. Kaplan-Meier analysis and the log-rank test showed statistically significant differences in 5-year survival rates and relapse between patients with tumour margins positive for c-erb-B2 amplification and those with margins that were negative (P = 0.002). H-ras and c-Myc alterations could not be associated with tumour behaviour. Molecular analysis of margins, targeting cancer genes, could identify additional, independent predictors of risk and outcome in OSCC.",
publisher = "Churchill Livingstone, Edinburgh",
journal = "International Journal of Oral & Maxillofacial Surgery",
title = "The amplification of c-erb-B2 in cancer-free surgical margins is a predictor of poor outcome in oral squamous cell carcinoma",
volume = "45",
number = "6",
pages = "700-705",
doi = "10.1016/j.ijom.2015.11.014"
}

Jelovac, D., Tepavčević, Z., Nikolić, N., Ilić, B., Eljabo, N., Popović, B., Čarkić, J., Konstantinović, V., Vukadinović, M., Miličić, B.,& Milašin, J.. (2016). The amplification of c-erb-B2 in cancer-free surgical margins is a predictor of poor outcome in oral squamous cell carcinoma. in International Journal of Oral & Maxillofacial Surgery
Churchill Livingstone, Edinburgh., 45(6), 700-705.
https://doi.org/10.1016/j.ijom.2015.11.014

Jelovac D, Tepavčević Z, Nikolić N, Ilić B, Eljabo N, Popović B, Čarkić J, Konstantinović V, Vukadinović M, Miličić B, Milašin J. The amplification of c-erb-B2 in cancer-free surgical margins is a predictor of poor outcome in oral squamous cell carcinoma. in International Journal of Oral & Maxillofacial Surgery. 2016;45(6):700-705.
doi:10.1016/j.ijom.2015.11.014 .

Jelovac, Drago, Tepavčević, Zvezdana, Nikolić, Nadja, Ilić, Branislav, Eljabo, Najib, Popović, Branka, Čarkić, Jelena, Konstantinović, Vitomir, Vukadinović, Miroslav, Miličić, Biljana, Milašin, Jelena, "The amplification of c-erb-B2 in cancer-free surgical margins is a predictor of poor outcome in oral squamous cell carcinoma" in International Journal of Oral & Maxillofacial Surgery, 45, no. 6 (2016):700-705,
https://doi.org/10.1016/j.ijom.2015.11.014 . .

TY  - JOUR
AU  - Čarkić, Jelena
AU  - Nikolić, Nadja
AU  - Radojević-Škodrić, Sanja
AU  - Kuzmanović-Pfićer, Jovana
AU  - Brajović, Gavrilo
AU  - Antunović, Marija
AU  - Milašin, Jelena
AU  - Popović, Branka
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2115
AB  - The aim of this study was to assess TERT-CLPTM1L single-nucleotide polymorphisms (SNPs) (rs402710 C/T in the CLPTM1L gene; rs2736100 A/C and rs2736098 G/A in the TERT gene) as risk factors for development of oral squamous cell carcinoma (OSCC), and to investigate the relationship between the analyzed polymorphisms, relative telomere length (RTL), telomerase expression and clinicopathologic characteristics of OSCC in a Serbian population. Paraffin-embedded tumor samples and buccal swabs from cancer-free controls were genotyped using PCR-RFLP, while tumor RTL values and telomerase expression were estimated by real-time PCR and immunohistochemistry, respectively. CLPTM1L rs402710 and TERT rs2736100 polymorphisms were associated with a significantly increased risk of OSCC, and TERT rs2736098 with a significantly decreased risk. No significant association was found between TERT-CLPTM1L polymorphisms, tumor RTL values, telomerase expression, and clinicopathologic features, although a trend towards longer telomeres was evident in telomerase-positive samples and less advanced tumors. Kaplan-Meier survival analysis showed that patients with longer telomeres in their tumors had significantly better overall survival than patients with shorter telomeres. Our research seems to provide strong evidence for an association between CLPTMIL rs402710C/T and TERT rs2736100A/C SNPs and the risk of OSSC, and suggests that higher tumor RTL values and positive hTERT expression may be applicable as early prognostic markers.
PB  - Nihon Univ, School Dentistry, Toyko
T2  - Journal of Oral Science
T1  - The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma
VL  - 58
IS  - 4
SP  - 449
EP  - 458
DO  - 10.2334/josnusd.16-0108
ER  - 

@article{
author = "Čarkić, Jelena and Nikolić, Nadja and Radojević-Škodrić, Sanja and Kuzmanović-Pfićer, Jovana and Brajović, Gavrilo and Antunović, Marija and Milašin, Jelena and Popović, Branka",
year = "2016",
abstract = "The aim of this study was to assess TERT-CLPTM1L single-nucleotide polymorphisms (SNPs) (rs402710 C/T in the CLPTM1L gene; rs2736100 A/C and rs2736098 G/A in the TERT gene) as risk factors for development of oral squamous cell carcinoma (OSCC), and to investigate the relationship between the analyzed polymorphisms, relative telomere length (RTL), telomerase expression and clinicopathologic characteristics of OSCC in a Serbian population. Paraffin-embedded tumor samples and buccal swabs from cancer-free controls were genotyped using PCR-RFLP, while tumor RTL values and telomerase expression were estimated by real-time PCR and immunohistochemistry, respectively. CLPTM1L rs402710 and TERT rs2736100 polymorphisms were associated with a significantly increased risk of OSCC, and TERT rs2736098 with a significantly decreased risk. No significant association was found between TERT-CLPTM1L polymorphisms, tumor RTL values, telomerase expression, and clinicopathologic features, although a trend towards longer telomeres was evident in telomerase-positive samples and less advanced tumors. Kaplan-Meier survival analysis showed that patients with longer telomeres in their tumors had significantly better overall survival than patients with shorter telomeres. Our research seems to provide strong evidence for an association between CLPTMIL rs402710C/T and TERT rs2736100A/C SNPs and the risk of OSSC, and suggests that higher tumor RTL values and positive hTERT expression may be applicable as early prognostic markers.",
publisher = "Nihon Univ, School Dentistry, Toyko",
journal = "Journal of Oral Science",
title = "The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma",
volume = "58",
number = "4",
pages = "449-458",
doi = "10.2334/josnusd.16-0108"
}

Čarkić, J., Nikolić, N., Radojević-Škodrić, S., Kuzmanović-Pfićer, J., Brajović, G., Antunović, M., Milašin, J.,& Popović, B.. (2016). The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma. in Journal of Oral Science
Nihon Univ, School Dentistry, Toyko., 58(4), 449-458.
https://doi.org/10.2334/josnusd.16-0108

Čarkić J, Nikolić N, Radojević-Škodrić S, Kuzmanović-Pfićer J, Brajović G, Antunović M, Milašin J, Popović B. The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma. in Journal of Oral Science. 2016;58(4):449-458.
doi:10.2334/josnusd.16-0108 .

Čarkić, Jelena, Nikolić, Nadja, Radojević-Škodrić, Sanja, Kuzmanović-Pfićer, Jovana, Brajović, Gavrilo, Antunović, Marija, Milašin, Jelena, Popović, Branka, "The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma" in Journal of Oral Science, 58, no. 4 (2016):449-458,
https://doi.org/10.2334/josnusd.16-0108 . .

TY  - JOUR
AU  - Brajović, Gavrilo
AU  - Popović, Branka
AU  - Puletić, Miljan
AU  - Kostić, Marija
AU  - Milašin, Jelena
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2155
AB  - Introduction Periodontal diseases are associated with the presence of elevated levels of bacteria within the gingival crevice. Objective The aim of this study was to evaluate a total amount of bacteria in subgingival plaque samples in patients with a periodontal disease. Methods A quantitative evaluation of total bacteria amount using quantitative real-time polymerase chain reaction (qRT-PCR) was performed on 20 samples of patients with ulceronecrotic periodontitis and on 10 samples of healthy subjects. The estimation of total bacterial amount was based on gene copy number for 16S rRNA that was determined by comparing to Ct values / gene copy number of the standard curve. Results A statistically significant difference between average gene copy number of total bacteria in periodontal patients (2.55×107) and healthy control (2.37×106) was found (p=0.01). Also, a trend of higher numbers of the gene copy in deeper periodontal lesions (>7 mm) was confirmed by a positive value of coefficient of correlation (r=0.073). Conclusion The quantitative estimation of total bacteria based on gene copy number could be an important additional tool in diagnosing periodontitis.
AB  - Uvod Parodontopatija se povezuje sa postojanjem povećanog broja bakterija u parodontalnom džepu. Cilj rada Cilj rada je bila kvantifikacija ukupnih bakterija u uzorcima subgingivalnog dentalnog plaka kod osoba obolelih od parodontopatije. Metode rada U 20 uzoraka subgingivalnog plaka ispitanika sa ulceronekroznom parodontopatijom i 10 uzoraka osoba sa zdravim parodoncijumom izvršena je kvantifikacija ukupnog broja bakterija korišćenjem metode qRT- PCR. Kvantifikacija bakterija je zasnovana na određivanju ukupnog broja genskih kopija za rRNK poređenjem sa CT vrednošću standarda. Rezultati Ustanovljena je statistički značajna razlika u prosečnom broju genskih kopija ukupnih bakterija između ispitanika sa parodontopatijom (2,55×107) i ispitanika kontrolne grupe (2,37×106), (p=0,01). Takođe, trend porasta broja genskih kopija ukupnih bakterija s povećanjem dubine parodontalnog džepa (>7 mm) potvrđen je pozitivnom vrednošću koeficijenta korelacije (p=0,073). Zaključak Procena ukupnog broja bakterija na osnovu broja genskih kopija za 16S rRNK može biti važan dodatni parametar u dijagnostikovanju parodontopatije.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Estimation of total bacteria by real-time PCR in patients with periodontal disease
T1  - Procena broja ukupnih bakterija primenom real-time PCR metode kod pacijenata sa parodontopatijom
VL  - 144
IS  - 1-2
SP  - 10
EP  - 14
DO  - 10.2298/SARH1602010B
ER  - 

@article{
author = "Brajović, Gavrilo and Popović, Branka and Puletić, Miljan and Kostić, Marija and Milašin, Jelena",
year = "2016",
abstract = "Introduction Periodontal diseases are associated with the presence of elevated levels of bacteria within the gingival crevice. Objective The aim of this study was to evaluate a total amount of bacteria in subgingival plaque samples in patients with a periodontal disease. Methods A quantitative evaluation of total bacteria amount using quantitative real-time polymerase chain reaction (qRT-PCR) was performed on 20 samples of patients with ulceronecrotic periodontitis and on 10 samples of healthy subjects. The estimation of total bacterial amount was based on gene copy number for 16S rRNA that was determined by comparing to Ct values / gene copy number of the standard curve. Results A statistically significant difference between average gene copy number of total bacteria in periodontal patients (2.55×107) and healthy control (2.37×106) was found (p=0.01). Also, a trend of higher numbers of the gene copy in deeper periodontal lesions (>7 mm) was confirmed by a positive value of coefficient of correlation (r=0.073). Conclusion The quantitative estimation of total bacteria based on gene copy number could be an important additional tool in diagnosing periodontitis., Uvod Parodontopatija se povezuje sa postojanjem povećanog broja bakterija u parodontalnom džepu. Cilj rada Cilj rada je bila kvantifikacija ukupnih bakterija u uzorcima subgingivalnog dentalnog plaka kod osoba obolelih od parodontopatije. Metode rada U 20 uzoraka subgingivalnog plaka ispitanika sa ulceronekroznom parodontopatijom i 10 uzoraka osoba sa zdravim parodoncijumom izvršena je kvantifikacija ukupnog broja bakterija korišćenjem metode qRT- PCR. Kvantifikacija bakterija je zasnovana na određivanju ukupnog broja genskih kopija za rRNK poređenjem sa CT vrednošću standarda. Rezultati Ustanovljena je statistički značajna razlika u prosečnom broju genskih kopija ukupnih bakterija između ispitanika sa parodontopatijom (2,55×107) i ispitanika kontrolne grupe (2,37×106), (p=0,01). Takođe, trend porasta broja genskih kopija ukupnih bakterija s povećanjem dubine parodontalnog džepa (>7 mm) potvrđen je pozitivnom vrednošću koeficijenta korelacije (p=0,073). Zaključak Procena ukupnog broja bakterija na osnovu broja genskih kopija za 16S rRNK može biti važan dodatni parametar u dijagnostikovanju parodontopatije.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Estimation of total bacteria by real-time PCR in patients with periodontal disease, Procena broja ukupnih bakterija primenom real-time PCR metode kod pacijenata sa parodontopatijom",
volume = "144",
number = "1-2",
pages = "10-14",
doi = "10.2298/SARH1602010B"
}

Brajović, G., Popović, B., Puletić, M., Kostić, M.,& Milašin, J.. (2016). Estimation of total bacteria by real-time PCR in patients with periodontal disease. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 144(1-2), 10-14.
https://doi.org/10.2298/SARH1602010B

Brajović G, Popović B, Puletić M, Kostić M, Milašin J. Estimation of total bacteria by real-time PCR in patients with periodontal disease. in Srpski arhiv za celokupno lekarstvo. 2016;144(1-2):10-14.
doi:10.2298/SARH1602010B .

Brajović, Gavrilo, Popović, Branka, Puletić, Miljan, Kostić, Marija, Milašin, Jelena, "Estimation of total bacteria by real-time PCR in patients with periodontal disease" in Srpski arhiv za celokupno lekarstvo, 144, no. 1-2 (2016):10-14,
https://doi.org/10.2298/SARH1602010B . .

TY  - JOUR
AU  - Brajović, Gavrilo
AU  - Nikolić-Jakoba, Nataša
AU  - Popović, Branka
AU  - Ilić, Vesna
AU  - Mojsilović, Sonja
AU  - Marković, Dragana
AU  - Milošević-Jovčić, Nadežda
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2046
AB  - Introduction Fibronectin (FN) can interact with immunoglobulin G (IgG) molecules affecting the process of physiological elimination and causing abnormal deposition of immune complexes. The aim of the study was to analyze interaction between FN fragments and IgG molecules with different glycosylation profiles in gingival crevicular fluid (GCF) of patients with periodontal disease and healthy controls. Material and Methods The study included 30 patients with moderate and advanced periodontitis and 22 healthy subjects. IgG and FN content in GCF were measured as well as the presence of FN and galactose expression on IgG molecules. Results IgG content in GCF was five times higher in patients with moderate (p lt 0.01) and eight time higher in patients with advanced periodontitis (p lt 0.001) compared to healthy subjects. Also, hypogalactosylated forms of IgG were found in higher concentration in GCF of patients with advanced periodontitis compared to moderate periodontitis and healthy subjects (p lt 0.05). FN fragments of molecular mass 48 - 53 kDa were the most commonly found fragments in all three groups. Furthermore, in patients with advanced periodontitis, fibronectin fragments were attached to IgG molecules. Conclusion IgG and FN fragments form complexes in GCF in patients with periodontal disease and healthy subjects.
AB  - Uvod Fibronektin može da interreaguje s molekulima imunoglobulina G (IgG) i utiče na normalan klirens ili poremećeno deponovanje imunskih kompleksa. Cilj ovog rada je bio da se ispita veza između fibronektina i IgG različitih glikoformi u gingivalnoj tečnosti osoba obolelih od parodontopatije i parodontalno zdravih ispitanika. Materijal i metode rada U studiju je uključeno 30 pacijenata s umerenom i uznapredovalom parodontopatijom i 22 parodontalno zdrave osobe. U gingivalnoj tečnosti određivan je sadržaj IgG i fibronektina dot blot i imunoblot tehnikama. IgG iz gingivalnih tečnosti su afinitetno izolovani i analizirani na prisustvo fibronektina i ekspresiju galaktoze. Rezultati Sadržaj IgG u gingivalnoj tečnosti osoba s umerenom parodontopatijom bio je oko pet puta veći u odnosu na sadržaj IgG kod zdravih osoba (p lt 0,01), dok je kod uznapredovalih oblika bio oko osam puta veći (p lt 0,001). Takođe, hipogalaktozilovane forme IgG su većoj meri postojale kod osoba sa uznapredovalom parodontopatijom u odnosu na zdrave i osobe s umerenom parodontopatijom (p lt 0,05). U sve tri analizirane grupe dominirali su fibronektinski fragmenti od 48 do 53 kDa. Uočeno je da su IgG izolovani iz gingivalne tečnosti vezani za fragmente fibronektina, pri čemu su IgG osoba sa uznapredovalom parodontopatijom, imali najveću količinu ovih vezanih fragmenata. Zaključak Dobijeni rezultati pokazuju da IgG gingivalne tečnosti zdravih i osoba s parodontopatijom formiraju komplekse sa fibronektinom.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease
T1  - Kompleksi fibronektinskih fragmenata i imunoglobulina G u gingivalnoj tečnosti osoba obolelih od parodontopatije
VL  - 62
IS  - 2
SP  - 55
EP  - 64
DO  - 10.1515/sdj-2015-0006
ER  - 

@article{
author = "Brajović, Gavrilo and Nikolić-Jakoba, Nataša and Popović, Branka and Ilić, Vesna and Mojsilović, Sonja and Marković, Dragana and Milošević-Jovčić, Nadežda",
year = "2015",
abstract = "Introduction Fibronectin (FN) can interact with immunoglobulin G (IgG) molecules affecting the process of physiological elimination and causing abnormal deposition of immune complexes. The aim of the study was to analyze interaction between FN fragments and IgG molecules with different glycosylation profiles in gingival crevicular fluid (GCF) of patients with periodontal disease and healthy controls. Material and Methods The study included 30 patients with moderate and advanced periodontitis and 22 healthy subjects. IgG and FN content in GCF were measured as well as the presence of FN and galactose expression on IgG molecules. Results IgG content in GCF was five times higher in patients with moderate (p lt 0.01) and eight time higher in patients with advanced periodontitis (p lt 0.001) compared to healthy subjects. Also, hypogalactosylated forms of IgG were found in higher concentration in GCF of patients with advanced periodontitis compared to moderate periodontitis and healthy subjects (p lt 0.05). FN fragments of molecular mass 48 - 53 kDa were the most commonly found fragments in all three groups. Furthermore, in patients with advanced periodontitis, fibronectin fragments were attached to IgG molecules. Conclusion IgG and FN fragments form complexes in GCF in patients with periodontal disease and healthy subjects., Uvod Fibronektin može da interreaguje s molekulima imunoglobulina G (IgG) i utiče na normalan klirens ili poremećeno deponovanje imunskih kompleksa. Cilj ovog rada je bio da se ispita veza između fibronektina i IgG različitih glikoformi u gingivalnoj tečnosti osoba obolelih od parodontopatije i parodontalno zdravih ispitanika. Materijal i metode rada U studiju je uključeno 30 pacijenata s umerenom i uznapredovalom parodontopatijom i 22 parodontalno zdrave osobe. U gingivalnoj tečnosti određivan je sadržaj IgG i fibronektina dot blot i imunoblot tehnikama. IgG iz gingivalnih tečnosti su afinitetno izolovani i analizirani na prisustvo fibronektina i ekspresiju galaktoze. Rezultati Sadržaj IgG u gingivalnoj tečnosti osoba s umerenom parodontopatijom bio je oko pet puta veći u odnosu na sadržaj IgG kod zdravih osoba (p lt 0,01), dok je kod uznapredovalih oblika bio oko osam puta veći (p lt 0,001). Takođe, hipogalaktozilovane forme IgG su većoj meri postojale kod osoba sa uznapredovalom parodontopatijom u odnosu na zdrave i osobe s umerenom parodontopatijom (p lt 0,05). U sve tri analizirane grupe dominirali su fibronektinski fragmenti od 48 do 53 kDa. Uočeno je da su IgG izolovani iz gingivalne tečnosti vezani za fragmente fibronektina, pri čemu su IgG osoba sa uznapredovalom parodontopatijom, imali najveću količinu ovih vezanih fragmenata. Zaključak Dobijeni rezultati pokazuju da IgG gingivalne tečnosti zdravih i osoba s parodontopatijom formiraju komplekse sa fibronektinom.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease, Kompleksi fibronektinskih fragmenata i imunoglobulina G u gingivalnoj tečnosti osoba obolelih od parodontopatije",
volume = "62",
number = "2",
pages = "55-64",
doi = "10.1515/sdj-2015-0006"
}

Brajović, G., Nikolić-Jakoba, N., Popović, B., Ilić, V., Mojsilović, S., Marković, D.,& Milošević-Jovčić, N.. (2015). Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 62(2), 55-64.
https://doi.org/10.1515/sdj-2015-0006

Brajović G, Nikolić-Jakoba N, Popović B, Ilić V, Mojsilović S, Marković D, Milošević-Jovčić N. Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease. in Stomatološki glasnik Srbije. 2015;62(2):55-64.
doi:10.1515/sdj-2015-0006 .

Brajović, Gavrilo, Nikolić-Jakoba, Nataša, Popović, Branka, Ilić, Vesna, Mojsilović, Sonja, Marković, Dragana, Milošević-Jovčić, Nadežda, "Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease" in Stomatološki glasnik Srbije, 62, no. 2 (2015):55-64,
https://doi.org/10.1515/sdj-2015-0006 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Knežević, Aleksandra
AU  - Karalić, Danijela
AU  - Soldatović, Ivan
AU  - Popović, Branka
AU  - Milašin, Jelena
AU  - Andrić, Miroslav
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1986
AB  - This study aimed to compare the levels of tumour necrosis factor-alpha (TNF-), interleukin-1 beta (IL-1) and interleukin-6 (IL-6) between apical periodontitis lesions with different clinical and histological features. Based on clinical data and history of disease, 100 human apical periodontitis lesions were categorised as either asymptomatic or symptomatic lesions. According to histological examination, lesions were divided into periapical granulomas and radicular cysts. Pulp tissues of 25 impacted wisdom teeth were used as controls. Homogenised tissue samples were centrifuged and supernatants were used for the determination of cytokine levels by enzyme-linked immunosorbent assay. Significantly higher levels of IL-1 and IL-6 were found in symptomatic lesions compared with asymptomatic lesions and control tissues (P lt 0.001, P lt 0.001, respectively). The concentration of IL-1 was significantly higher in radicular cysts compared with periapical granulomas (P=0.003). Symptomatic lesions, as judged by high local production of IL-1 and IL-6, represent an immunologically active stage of the disease.
PB  - Wiley, Hoboken
T2  - Australian Endodontic Journal
T1  - Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings
VL  - 41
IS  - 2
SP  - 72
EP  - 77
DO  - 10.1111/aej.12072
ER  - 

@article{
author = "Jakovljević, Aleksandar and Knežević, Aleksandra and Karalić, Danijela and Soldatović, Ivan and Popović, Branka and Milašin, Jelena and Andrić, Miroslav",
year = "2015",
abstract = "This study aimed to compare the levels of tumour necrosis factor-alpha (TNF-), interleukin-1 beta (IL-1) and interleukin-6 (IL-6) between apical periodontitis lesions with different clinical and histological features. Based on clinical data and history of disease, 100 human apical periodontitis lesions were categorised as either asymptomatic or symptomatic lesions. According to histological examination, lesions were divided into periapical granulomas and radicular cysts. Pulp tissues of 25 impacted wisdom teeth were used as controls. Homogenised tissue samples were centrifuged and supernatants were used for the determination of cytokine levels by enzyme-linked immunosorbent assay. Significantly higher levels of IL-1 and IL-6 were found in symptomatic lesions compared with asymptomatic lesions and control tissues (P lt 0.001, P lt 0.001, respectively). The concentration of IL-1 was significantly higher in radicular cysts compared with periapical granulomas (P=0.003). Symptomatic lesions, as judged by high local production of IL-1 and IL-6, represent an immunologically active stage of the disease.",
publisher = "Wiley, Hoboken",
journal = "Australian Endodontic Journal",
title = "Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings",
volume = "41",
number = "2",
pages = "72-77",
doi = "10.1111/aej.12072"
}

Jakovljević, A., Knežević, A., Karalić, D., Soldatović, I., Popović, B., Milašin, J.,& Andrić, M.. (2015). Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings. in Australian Endodontic Journal
Wiley, Hoboken., 41(2), 72-77.
https://doi.org/10.1111/aej.12072

Jakovljević A, Knežević A, Karalić D, Soldatović I, Popović B, Milašin J, Andrić M. Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings. in Australian Endodontic Journal. 2015;41(2):72-77.
doi:10.1111/aej.12072 .

Jakovljević, Aleksandar, Knežević, Aleksandra, Karalić, Danijela, Soldatović, Ivan, Popović, Branka, Milašin, Jelena, Andrić, Miroslav, "Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings" in Australian Endodontic Journal, 41, no. 2 (2015):72-77,
https://doi.org/10.1111/aej.12072 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Jauković, Aleksandra
AU  - Popović, Branka
AU  - Krstić, Jelena
AU  - Mojsilović, Slavko
AU  - Okic-Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Santibanez, Juan Francisco
AU  - Bugarski, Diana
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2018
AB  - Aims: In vitro expansion changes replication and differentiation capacity of mesenchymal stem cells (MSCs), increasing challenges and risks, while limiting the sufficient number of MSCs required for cytotherapy. Here, we characterized and compared proliferation, differentiation, telomere length and pluripotency marker expression in MSCs of various origins. Main methods: Immunophenotyping, proliferation and differentiation assays were performed. Pluripotency marker (Nanog, Oct-4, SOX-2, SSEA-4) expression was determined by immunofluorescence. Quantitative PCR was performed for relative telomere length (RTL) analyses, while expression of relevant genes for pluripotency markers, differentiation state (Cbfa1, human placental alkaline phosphatase, peroxisome proliferator activated receptor, Sox9 and Collagen II a1), and telomerase reverse transcriptase (hTERT) was determined by semiquantitative RT-PCR. Key findings: Peripheral blood MSCs (PB-MSCs) and umbilical cord MSCs (UC-MSCs) showed the highest, while periodontal ligament MSCs (PDL-MSCs) and adipose tissue MSCs (AT-MSCs) the lowest values of both the replication potential and RTL. Although MSCs from exfoliated deciduous teeth (SHEDs), PDL-MSCs and AT-MSCs showed higher mRNA expression of pluripotency markers, all MSCs expressed pluripotency marker proteins. SHEDs and PDL-MSCs showed prominent capacity for osteogenesis, PB-MSCs and UC-MSCs showed strengthened adipogenic differentiation potential, while AT-MSCs displayed similar differentiation into both lines. Significance: The MSCs populations derived from different sources, although displaying similar phenotype, exhibited high degree of variability regarding biological properties related to their self-renewal and differentiation capacity. These data indicate that for more accurate use in cell therapy, individualities of MSCs isolated from different tissues should be identified and taken into consideration when planning their use in clinical protocols.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression
VL  - 141
SP  - 61
EP  - 73
DO  - 10.1016/j.lfs.2015.09.019
ER  - 

@article{
author = "Trivanović, Drenka and Jauković, Aleksandra and Popović, Branka and Krstić, Jelena and Mojsilović, Slavko and Okic-Đorđević, Ivana and Kukolj, Tamara and Obradović, Hristina and Santibanez, Juan Francisco and Bugarski, Diana",
year = "2015",
abstract = "Aims: In vitro expansion changes replication and differentiation capacity of mesenchymal stem cells (MSCs), increasing challenges and risks, while limiting the sufficient number of MSCs required for cytotherapy. Here, we characterized and compared proliferation, differentiation, telomere length and pluripotency marker expression in MSCs of various origins. Main methods: Immunophenotyping, proliferation and differentiation assays were performed. Pluripotency marker (Nanog, Oct-4, SOX-2, SSEA-4) expression was determined by immunofluorescence. Quantitative PCR was performed for relative telomere length (RTL) analyses, while expression of relevant genes for pluripotency markers, differentiation state (Cbfa1, human placental alkaline phosphatase, peroxisome proliferator activated receptor, Sox9 and Collagen II a1), and telomerase reverse transcriptase (hTERT) was determined by semiquantitative RT-PCR. Key findings: Peripheral blood MSCs (PB-MSCs) and umbilical cord MSCs (UC-MSCs) showed the highest, while periodontal ligament MSCs (PDL-MSCs) and adipose tissue MSCs (AT-MSCs) the lowest values of both the replication potential and RTL. Although MSCs from exfoliated deciduous teeth (SHEDs), PDL-MSCs and AT-MSCs showed higher mRNA expression of pluripotency markers, all MSCs expressed pluripotency marker proteins. SHEDs and PDL-MSCs showed prominent capacity for osteogenesis, PB-MSCs and UC-MSCs showed strengthened adipogenic differentiation potential, while AT-MSCs displayed similar differentiation into both lines. Significance: The MSCs populations derived from different sources, although displaying similar phenotype, exhibited high degree of variability regarding biological properties related to their self-renewal and differentiation capacity. These data indicate that for more accurate use in cell therapy, individualities of MSCs isolated from different tissues should be identified and taken into consideration when planning their use in clinical protocols.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression",
volume = "141",
pages = "61-73",
doi = "10.1016/j.lfs.2015.09.019"
}

Trivanović, D., Jauković, A., Popović, B., Krstić, J., Mojsilović, S., Okic-Đorđević, I., Kukolj, T., Obradović, H., Santibanez, J. F.,& Bugarski, D.. (2015). Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 141, 61-73.
https://doi.org/10.1016/j.lfs.2015.09.019

Trivanović D, Jauković A, Popović B, Krstić J, Mojsilović S, Okic-Đorđević I, Kukolj T, Obradović H, Santibanez JF, Bugarski D. Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression. in Life Sciences. 2015;141:61-73.
doi:10.1016/j.lfs.2015.09.019 .

Trivanović, Drenka, Jauković, Aleksandra, Popović, Branka, Krstić, Jelena, Mojsilović, Slavko, Okic-Đorđević, Ivana, Kukolj, Tamara, Obradović, Hristina, Santibanez, Juan Francisco, Bugarski, Diana, "Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression" in Life Sciences, 141 (2015):61-73,
https://doi.org/10.1016/j.lfs.2015.09.019 . .

TY  - JOUR
AU  - Petrović-Matić, Sanja
AU  - Zelić, Ksenija
AU  - Milašin, Jelena
AU  - Popović, Branka
AU  - Pucar, Ana
AU  - Zelić, Obrad
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1917
AB  - Introduction Pathogenesis and some characteristics of periodontitis cannot be fully explained by bacterial etiology alone. Herpes viruses may bridge the gap between clinical characteristics and molecular understanding of periodontal destruction. Objective The aim of this study was to investigate the prevalence of herpes simplex virus type 1 (HSV-1) in gingival crevicular fluid (GCF) of healthy and damaged periodontium in Serbian population and to explore potential correlation between the presence of this virus and the level of periodontal destruction. Methods Samples were collected from gingival sulcus/periodontal pockets by sterile paper points and the presence of viral DNA in gingival crevicular fluid was assessed by PCR. Results There was no statistically significant difference in HSV-1 in presence between periodontitis patients (PG=38.9%) and healthy controls (HC=32.3%), (Chi-square test, with Yates' correction p=0.7574). However, HSV-1 positive patients showed significantly higher values of parameters of periodontal destruction (PPD=7.11±2.52, CAL=5.46±2.34) than periodontitis patients without HSV-1 in gingival crevicular fluid (PPD=4.70±1.79, CAL=3.39±2.65) (p values respectively, p=0.002 and p=0.023, Independent Samples T-Test). HSV-1 occurred more often in deeper (PPD≥6 mm) (69.2%) than in shallow pockets (3 mm lt PPD lt 6 mm) (18.2%) (Chi-square test, with Yates' correction, p=0.008). Plaque index was lower in the HSV-1 positive group (0.84± 0.69 vs. 1.43±0.76, p=0.023, Independent Samples T-Test). Conclusion This study demonstrated that the presence of HSV-1 in the gingival crevicular fluid coincides with a higher degree of tissue destruction in patients with periodontitis.
AB  - Uvod Patogeneza i neke kliničke odlike parodontopatije ne mogu se do kraja objasniti bakterijskom etiologijom. Herpesvirusi bi mogli da premoste jaz koji postoji između kliničkih osobina i poznavanja patogenetskih mehanizama parodontopatije na molekularnom nivou. Cilj rada Cilj ove studije bio je određivanje prevalencije virusa herpes simplex tip 1 (HSV-1) u zdravom i obolelom parodoncijumu osoba u Srbiji, kao i utvrđivanje moguće korelacije između postojanja ovih virusa i stepena oštećenja parodoncijuma. Metode rada Uzorci su uzimani papirnim poenima iz gingivalnih sulkusa ili parodontalnih džepova, a virus je otkrivan reakcijom lančanog umnožavanja molekula DNK (engl. polymerase chain reaction - PCR). Rezultati Nije utvrđena statistički značajna razlika u prevalenciji HSV-1 između osoba s parodontopatijom (32,3%) i zdravim parodoncijumom (38,9%); χ2-test sa Jejtsovom (Yates) korekcijom: p=0,7574. U grupi ispitanika s parodontopatijom parametri koji označavaju stepen oštećenja (dubina parodontalnog džepa - DPDž; nivo pripojnog epitela - NPE) bili su značajno veći kod onih s otkrivenim virusom (DPDž: 7,11±2,52 mm; NPE: 5,46±2,34), nego kod ispitanika bez virusa u uzorcima gingivalne tečnosti (DPDž=4,70±1,79 mm; NPE=3,39±2,65); Studentov t-test za nevezane uzorke: p=0,002, odnosno p=0,023. U dubljim parodontalnim džepovima (DPDž≥6 mm) HSV-1 je otkriven statistički značajno češće (69,2%) nego u plićim džepovima (DPDž=3-6 mm) (18,2%); χ2-test sa Jejtsovom korekcijom: p=0,008. Prosečne vrednosti plak-indeksa bile su niže kod ispitanika sa HSV-1 (0,84±0,69) u poređenju sa ispitanicima kod kojih ovaj virus nije otkriven (1,43±0,76); Studentov t-test za nevezane uzorke: p=0,023. Zaključak Prikazana studija pokazala je da je postojanje HSV-1 povezano sa nivoom oštećenja tkiva kod osoba s parodontopatijom.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction
T1  - Otkrivanje virusa herpes simplex tip 1 u gingivalnoj tečnosti sulkusa ili parodontalnog džepa lančanom reakcijom polimeraze
VL  - 142
IS  - 5-6
SP  - 296
EP  - 300
DO  - 10.2298/SARH1406296M
ER  - 

@article{
author = "Petrović-Matić, Sanja and Zelić, Ksenija and Milašin, Jelena and Popović, Branka and Pucar, Ana and Zelić, Obrad",
year = "2014",
abstract = "Introduction Pathogenesis and some characteristics of periodontitis cannot be fully explained by bacterial etiology alone. Herpes viruses may bridge the gap between clinical characteristics and molecular understanding of periodontal destruction. Objective The aim of this study was to investigate the prevalence of herpes simplex virus type 1 (HSV-1) in gingival crevicular fluid (GCF) of healthy and damaged periodontium in Serbian population and to explore potential correlation between the presence of this virus and the level of periodontal destruction. Methods Samples were collected from gingival sulcus/periodontal pockets by sterile paper points and the presence of viral DNA in gingival crevicular fluid was assessed by PCR. Results There was no statistically significant difference in HSV-1 in presence between periodontitis patients (PG=38.9%) and healthy controls (HC=32.3%), (Chi-square test, with Yates' correction p=0.7574). However, HSV-1 positive patients showed significantly higher values of parameters of periodontal destruction (PPD=7.11±2.52, CAL=5.46±2.34) than periodontitis patients without HSV-1 in gingival crevicular fluid (PPD=4.70±1.79, CAL=3.39±2.65) (p values respectively, p=0.002 and p=0.023, Independent Samples T-Test). HSV-1 occurred more often in deeper (PPD≥6 mm) (69.2%) than in shallow pockets (3 mm lt PPD lt 6 mm) (18.2%) (Chi-square test, with Yates' correction, p=0.008). Plaque index was lower in the HSV-1 positive group (0.84± 0.69 vs. 1.43±0.76, p=0.023, Independent Samples T-Test). Conclusion This study demonstrated that the presence of HSV-1 in the gingival crevicular fluid coincides with a higher degree of tissue destruction in patients with periodontitis., Uvod Patogeneza i neke kliničke odlike parodontopatije ne mogu se do kraja objasniti bakterijskom etiologijom. Herpesvirusi bi mogli da premoste jaz koji postoji između kliničkih osobina i poznavanja patogenetskih mehanizama parodontopatije na molekularnom nivou. Cilj rada Cilj ove studije bio je određivanje prevalencije virusa herpes simplex tip 1 (HSV-1) u zdravom i obolelom parodoncijumu osoba u Srbiji, kao i utvrđivanje moguće korelacije između postojanja ovih virusa i stepena oštećenja parodoncijuma. Metode rada Uzorci su uzimani papirnim poenima iz gingivalnih sulkusa ili parodontalnih džepova, a virus je otkrivan reakcijom lančanog umnožavanja molekula DNK (engl. polymerase chain reaction - PCR). Rezultati Nije utvrđena statistički značajna razlika u prevalenciji HSV-1 između osoba s parodontopatijom (32,3%) i zdravim parodoncijumom (38,9%); χ2-test sa Jejtsovom (Yates) korekcijom: p=0,7574. U grupi ispitanika s parodontopatijom parametri koji označavaju stepen oštećenja (dubina parodontalnog džepa - DPDž; nivo pripojnog epitela - NPE) bili su značajno veći kod onih s otkrivenim virusom (DPDž: 7,11±2,52 mm; NPE: 5,46±2,34), nego kod ispitanika bez virusa u uzorcima gingivalne tečnosti (DPDž=4,70±1,79 mm; NPE=3,39±2,65); Studentov t-test za nevezane uzorke: p=0,002, odnosno p=0,023. U dubljim parodontalnim džepovima (DPDž≥6 mm) HSV-1 je otkriven statistički značajno češće (69,2%) nego u plićim džepovima (DPDž=3-6 mm) (18,2%); χ2-test sa Jejtsovom korekcijom: p=0,008. Prosečne vrednosti plak-indeksa bile su niže kod ispitanika sa HSV-1 (0,84±0,69) u poređenju sa ispitanicima kod kojih ovaj virus nije otkriven (1,43±0,76); Studentov t-test za nevezane uzorke: p=0,023. Zaključak Prikazana studija pokazala je da je postojanje HSV-1 povezano sa nivoom oštećenja tkiva kod osoba s parodontopatijom.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction, Otkrivanje virusa herpes simplex tip 1 u gingivalnoj tečnosti sulkusa ili parodontalnog džepa lančanom reakcijom polimeraze",
volume = "142",
number = "5-6",
pages = "296-300",
doi = "10.2298/SARH1406296M"
}

Petrović-Matić, S., Zelić, K., Milašin, J., Popović, B., Pucar, A.,& Zelić, O.. (2014). Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 142(5-6), 296-300.
https://doi.org/10.2298/SARH1406296M

Petrović-Matić S, Zelić K, Milašin J, Popović B, Pucar A, Zelić O. Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction. in Srpski arhiv za celokupno lekarstvo. 2014;142(5-6):296-300.
doi:10.2298/SARH1406296M .

Petrović-Matić, Sanja, Zelić, Ksenija, Milašin, Jelena, Popović, Branka, Pucar, Ana, Zelić, Obrad, "Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction" in Srpski arhiv za celokupno lekarstvo, 142, no. 5-6 (2014):296-300,
https://doi.org/10.2298/SARH1406296M . .

TY  - JOUR
AU  - Živković-Sandić, Marija
AU  - Popović, Branka
AU  - Čarkić, Jelena
AU  - Nikolić, Nadja
AU  - Glišić, Branislav
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1916
AB  - Introduction The placement of fixed orthodontic appliances may lead to increased plaque accumulation and changes in subgingival microflora. Objective The aim of this study was to examine the changes in frequency of subgingival microflora that occur after placement and removal of fixed orthodontic appliance using polymerase chain reaction (PCR). Methods This study included 33 orthodontic patients, who were divided into two groups. Subgingival plaque samples were collected from the right upper incisor (U1) and right upper first molar (U6). In group A, the samples were taken three times: before placement appliance (T1), after one month (T2), and after 3 months (T3). In group B the samples were also taken three times: before appliance removal (T1), after one month (T2), and after three months (T3). PCR method was used to determine the presence of P. gingivalis, A. actinomycetemcomitans, T. forsythia, and P. intermedia. Results In group A the frequency of P. gingivalis showed statistically significant decrease at U1 (p=0.049) and U6 (p=0.008), from T1 to T2, and at U1 (p=0.048) from T1 to T3. In group B only the frequency of T. forsythia showed a statistically significant decrease, at U6 (T1 vs. T2, p=0.004; T1 vs. T3, p=0.0003). Regarding other analyzed bacteria, changes in the presence were noticed but no statistical significance was found. Conclusion Placement of fixed appliances may have an impact on subgingival microflora, but in the first months after the placement and removal of the appliance changes were not significant, probably due to good oral hygiene.
AB  - Uvod Postavka fiksnih ortodontskih aparata može dovesti do povećanog nagomilavanja plaka i promena u subgingivalnoj mikroflori. Cilj rada Cilj ovog rada bio je da se ispitaju promene subgingivalne mikroflore nakon postavke i uklanjanja fiksnih ortodontskih aparata primenom reakcije lančanog umnožavanja molekula DNK (engl. polymerase chain reaction - PCR). Metode rada Studija je obuhvatila 33 pacijenta koja su svrstana u dve grupe (A i B). Uzorci plaka su uzeti iz subgingivalnog prostora desnog gornjeg centralnog sekutića (U1) i desnog gornjeg prvog kutnjaka (U6). U grupi A uzorci su uzimani pre postavke fiksnog aparata (T1), mesec dana posle postavke (T2) i tri meseca od postavke (T3). U grupi B uzorci su uzimani pre uklanjanja aparata (T1), mesec dana posle uklanjanja (T2) i tri meseca nakon uklanjanja (T3). Primenom metode PCR analizirano je postojanje mikroorganizama: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythia i Prevotella intermedia. Rezultati U grupi A učestalost P. gingivalis pokazala je statistički značajno smanjenje na oba zuba (U1: p=0,049; U6: p=0,008) u vremenskom intervalu od T1 do T2. Uočeno je i statistički značajno smanjenje zastupljenosti ove bakterije na zubu U1 u intervalu od T1 do T3 (p=0,048). U grupi B samo se učestalost T. forsythia statistički značajno smanjila na zubu U6 u intervalu od T1 do T2 (p=0,004) i od T1 do T3 (p=0,0003). Učestalosti ostalih bakterija u obe grupe ispitanika nisu pokazale statistički značajne promene. Zaključak Postavka fiksnih aparata može da utiče na sastav subgingivalne mikroflore, ali u prvim mesecima nakon postavke i uklanjanja aparata uglavnom nisu uočene statistički značajne promene, verovatno zbog dobre oralne higijene.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Changes in subgingival microflora after placement and removal of fixed orthodontic appliances
T1  - Promene subgingivalne mikroflore nakon postavke i uklanjanja fiksnih ortodontskih aparata
VL  - 142
IS  - 5-6
SP  - 301
EP  - 305
DO  - 10.2298/SARH1406301Z
ER  - 

@article{
author = "Živković-Sandić, Marija and Popović, Branka and Čarkić, Jelena and Nikolić, Nadja and Glišić, Branislav",
year = "2014",
abstract = "Introduction The placement of fixed orthodontic appliances may lead to increased plaque accumulation and changes in subgingival microflora. Objective The aim of this study was to examine the changes in frequency of subgingival microflora that occur after placement and removal of fixed orthodontic appliance using polymerase chain reaction (PCR). Methods This study included 33 orthodontic patients, who were divided into two groups. Subgingival plaque samples were collected from the right upper incisor (U1) and right upper first molar (U6). In group A, the samples were taken three times: before placement appliance (T1), after one month (T2), and after 3 months (T3). In group B the samples were also taken three times: before appliance removal (T1), after one month (T2), and after three months (T3). PCR method was used to determine the presence of P. gingivalis, A. actinomycetemcomitans, T. forsythia, and P. intermedia. Results In group A the frequency of P. gingivalis showed statistically significant decrease at U1 (p=0.049) and U6 (p=0.008), from T1 to T2, and at U1 (p=0.048) from T1 to T3. In group B only the frequency of T. forsythia showed a statistically significant decrease, at U6 (T1 vs. T2, p=0.004; T1 vs. T3, p=0.0003). Regarding other analyzed bacteria, changes in the presence were noticed but no statistical significance was found. Conclusion Placement of fixed appliances may have an impact on subgingival microflora, but in the first months after the placement and removal of the appliance changes were not significant, probably due to good oral hygiene., Uvod Postavka fiksnih ortodontskih aparata može dovesti do povećanog nagomilavanja plaka i promena u subgingivalnoj mikroflori. Cilj rada Cilj ovog rada bio je da se ispitaju promene subgingivalne mikroflore nakon postavke i uklanjanja fiksnih ortodontskih aparata primenom reakcije lančanog umnožavanja molekula DNK (engl. polymerase chain reaction - PCR). Metode rada Studija je obuhvatila 33 pacijenta koja su svrstana u dve grupe (A i B). Uzorci plaka su uzeti iz subgingivalnog prostora desnog gornjeg centralnog sekutića (U1) i desnog gornjeg prvog kutnjaka (U6). U grupi A uzorci su uzimani pre postavke fiksnog aparata (T1), mesec dana posle postavke (T2) i tri meseca od postavke (T3). U grupi B uzorci su uzimani pre uklanjanja aparata (T1), mesec dana posle uklanjanja (T2) i tri meseca nakon uklanjanja (T3). Primenom metode PCR analizirano je postojanje mikroorganizama: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythia i Prevotella intermedia. Rezultati U grupi A učestalost P. gingivalis pokazala je statistički značajno smanjenje na oba zuba (U1: p=0,049; U6: p=0,008) u vremenskom intervalu od T1 do T2. Uočeno je i statistički značajno smanjenje zastupljenosti ove bakterije na zubu U1 u intervalu od T1 do T3 (p=0,048). U grupi B samo se učestalost T. forsythia statistički značajno smanjila na zubu U6 u intervalu od T1 do T2 (p=0,004) i od T1 do T3 (p=0,0003). Učestalosti ostalih bakterija u obe grupe ispitanika nisu pokazale statistički značajne promene. Zaključak Postavka fiksnih aparata može da utiče na sastav subgingivalne mikroflore, ali u prvim mesecima nakon postavke i uklanjanja aparata uglavnom nisu uočene statistički značajne promene, verovatno zbog dobre oralne higijene.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Changes in subgingival microflora after placement and removal of fixed orthodontic appliances, Promene subgingivalne mikroflore nakon postavke i uklanjanja fiksnih ortodontskih aparata",
volume = "142",
number = "5-6",
pages = "301-305",
doi = "10.2298/SARH1406301Z"
}

Živković-Sandić, M., Popović, B., Čarkić, J., Nikolić, N.,& Glišić, B.. (2014). Changes in subgingival microflora after placement and removal of fixed orthodontic appliances. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 142(5-6), 301-305.
https://doi.org/10.2298/SARH1406301Z

Živković-Sandić M, Popović B, Čarkić J, Nikolić N, Glišić B. Changes in subgingival microflora after placement and removal of fixed orthodontic appliances. in Srpski arhiv za celokupno lekarstvo. 2014;142(5-6):301-305.
doi:10.2298/SARH1406301Z .

Živković-Sandić, Marija, Popović, Branka, Čarkić, Jelena, Nikolić, Nadja, Glišić, Branislav, "Changes in subgingival microflora after placement and removal of fixed orthodontic appliances" in Srpski arhiv za celokupno lekarstvo, 142, no. 5-6 (2014):301-305,
https://doi.org/10.2298/SARH1406301Z . .

TY  - JOUR
AU  - Stojanović, Nikola
AU  - Krunić, Jelena
AU  - Popović, Branka
AU  - Stojičić, Sonja
AU  - Živković, Slavoljub
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1912
AB  - Introduction Because apical periodontitis is recognizably an infectious disease, elimination or reduction of intracanal bacteria is of utmost importance for optimum treatment outcome. Objective The prevalence of Enterococcus faecalis and Porphyromonas gingivalis in infected root canals was studied Also, the effect of endodontic therapy by using intracanal medicaments, calcium hydroxide paste (CH) or gutta-percha points containing calcium hydroxide (CH-GP) or chlorhexidine (CHX-GP) on these microorganisms was assessed by polymerase chain reaction (PCR) assay. Methods Fifty-one patients with chronic apical periodontitis were randomly allocated in one of the following groups according to the intracanal medicament used: CH, CH-GP and CHX-GP group. Bacterial samples were taken upon access (S1), after chemomechanical instrumentation (S2) and after 15-day medication (S3). PCR assay was used to detect the presence of selected bacteria. Results E. faecalis was detected in 49% (25/51) and P. gingivalis in 17.6% (9/51) of the samples. Samples which showed no bacterial presence at S1 were excluded from further analysis. Overall analysis of all 29 samples revealed significant differences between S1 and S2 (p lt 0.001), S2 and S3 (p lt 0.05), and S1 and S3 (p lt 0.001). When distinction was made between the intracanal medications, there was a significant difference in the number of PCR positive samples between S1 and S2, S1 and S3, but not between S2 and S3 samples. Conclusion E. faecalis is more prevalent than P. gingivalis in primary endodontic infection. Intracanal medication in conduction with instrumentation and irrigation efficiently eliminates E. faecalis and P. gingivalis from infected root canals.
AB  - Uvod Budući da je apeksni parodontitis oboljenje koje nastaje usled postojanja infekcije, za postizanje optimalnog ishoda lečenja najznačajnije je uklanjanje bakterija iz kanala korena zuba ili barem smanjenje njihovog broja. Cilj rada Cilj ovog rada je bio da se ispita prevalencija bakterija Enterococcus faecalis i Porphyromonas gingivalis u inficiranim kanalima korena zuba i ustanovi efekat endodontskog lečenja primenom intrakanalnih medikamenata - kalcijum-hidroksidne suspenzije (CH), gutaperka-poena na bazi kalcijum-hidroksida (CH-GP) ili gutaperka-poena na bazi hlorheksidina (CHX-GP) - na ove mikroorganizme metodom lančane reakcije polimerizacije (PCR). Metode rada Istraživanjem je obuhvaćena 51 osoba koja je imala zub s hroničnim apeksnim parodontitisom. Ispitanici su metodom slučajnog uzorka svrstani u tri grupe u zavisnosti od vrste primenjenog intrakanalnog medikamenta (CH, CH-GP i CHX-GP). Bakterijski uzorci iz kanala korena sakupljani su pri inicijalnom ulasku u kanal korena (S1), posle hemomehaničke obrade (S2) i posle petnaestodnevnog lečenja (S3). PCR analiza je korišćena za određivanje prisustva ispitivanih bakterija. Rezultati E. faecalis je izolovan iz 25 (49%) uzoraka, a P. gingivalis iz devet (17,6%). Uzorci u kojima nisu nađene bakterije u S1 fazi isključeni su iz dalje analize. Analiza svih 29 uzoraka je ukazala na statistički značajnu razliku između S1 i S2 uzoraka (p lt 0,001), S2 i S3 (p lt 0,05) i S1 i S3 (p lt 0,001). Kada se uzme u obzir vrsta intrakanalnog medikamenta, statistički značajna razlika u broju PCR-pozitivnih uzoraka zabeležena je između S1 i S2, S1 i S3, ali ne i između S2 i S3. Zaključak U primarnoj endodontskoj infekciji E. faecalis se češće javlja od P. gingivalis. Intrakanalna medikacija zajedno s instrumentacijom i irigacijom efikasno uklanja E. faecalis i P. gingivalis iz inficiranih kanala korena.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Prevalence of Enterococcus faecalis and Porphyromonas gingivalis in infected root canals and their susceptibility to endodontic treatment procedures: A molecular study
T1  - Prevalencija bakterija Enterococcus faecalis i Porphyromonas gingivalis u inficiranim kanalima korena zuba i njihova osetljivost na endodontsko lečenje - molekularna studija
VL  - 142
IS  - 9-10
SP  - 535
EP  - 541
DO  - 10.2298/SARH1410535S
ER  - 

@article{
author = "Stojanović, Nikola and Krunić, Jelena and Popović, Branka and Stojičić, Sonja and Živković, Slavoljub",
year = "2014",
abstract = "Introduction Because apical periodontitis is recognizably an infectious disease, elimination or reduction of intracanal bacteria is of utmost importance for optimum treatment outcome. Objective The prevalence of Enterococcus faecalis and Porphyromonas gingivalis in infected root canals was studied Also, the effect of endodontic therapy by using intracanal medicaments, calcium hydroxide paste (CH) or gutta-percha points containing calcium hydroxide (CH-GP) or chlorhexidine (CHX-GP) on these microorganisms was assessed by polymerase chain reaction (PCR) assay. Methods Fifty-one patients with chronic apical periodontitis were randomly allocated in one of the following groups according to the intracanal medicament used: CH, CH-GP and CHX-GP group. Bacterial samples were taken upon access (S1), after chemomechanical instrumentation (S2) and after 15-day medication (S3). PCR assay was used to detect the presence of selected bacteria. Results E. faecalis was detected in 49% (25/51) and P. gingivalis in 17.6% (9/51) of the samples. Samples which showed no bacterial presence at S1 were excluded from further analysis. Overall analysis of all 29 samples revealed significant differences between S1 and S2 (p lt 0.001), S2 and S3 (p lt 0.05), and S1 and S3 (p lt 0.001). When distinction was made between the intracanal medications, there was a significant difference in the number of PCR positive samples between S1 and S2, S1 and S3, but not between S2 and S3 samples. Conclusion E. faecalis is more prevalent than P. gingivalis in primary endodontic infection. Intracanal medication in conduction with instrumentation and irrigation efficiently eliminates E. faecalis and P. gingivalis from infected root canals., Uvod Budući da je apeksni parodontitis oboljenje koje nastaje usled postojanja infekcije, za postizanje optimalnog ishoda lečenja najznačajnije je uklanjanje bakterija iz kanala korena zuba ili barem smanjenje njihovog broja. Cilj rada Cilj ovog rada je bio da se ispita prevalencija bakterija Enterococcus faecalis i Porphyromonas gingivalis u inficiranim kanalima korena zuba i ustanovi efekat endodontskog lečenja primenom intrakanalnih medikamenata - kalcijum-hidroksidne suspenzije (CH), gutaperka-poena na bazi kalcijum-hidroksida (CH-GP) ili gutaperka-poena na bazi hlorheksidina (CHX-GP) - na ove mikroorganizme metodom lančane reakcije polimerizacije (PCR). Metode rada Istraživanjem je obuhvaćena 51 osoba koja je imala zub s hroničnim apeksnim parodontitisom. Ispitanici su metodom slučajnog uzorka svrstani u tri grupe u zavisnosti od vrste primenjenog intrakanalnog medikamenta (CH, CH-GP i CHX-GP). Bakterijski uzorci iz kanala korena sakupljani su pri inicijalnom ulasku u kanal korena (S1), posle hemomehaničke obrade (S2) i posle petnaestodnevnog lečenja (S3). PCR analiza je korišćena za određivanje prisustva ispitivanih bakterija. Rezultati E. faecalis je izolovan iz 25 (49%) uzoraka, a P. gingivalis iz devet (17,6%). Uzorci u kojima nisu nađene bakterije u S1 fazi isključeni su iz dalje analize. Analiza svih 29 uzoraka je ukazala na statistički značajnu razliku između S1 i S2 uzoraka (p lt 0,001), S2 i S3 (p lt 0,05) i S1 i S3 (p lt 0,001). Kada se uzme u obzir vrsta intrakanalnog medikamenta, statistički značajna razlika u broju PCR-pozitivnih uzoraka zabeležena je između S1 i S2, S1 i S3, ali ne i između S2 i S3. Zaključak U primarnoj endodontskoj infekciji E. faecalis se češće javlja od P. gingivalis. Intrakanalna medikacija zajedno s instrumentacijom i irigacijom efikasno uklanja E. faecalis i P. gingivalis iz inficiranih kanala korena.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Prevalence of Enterococcus faecalis and Porphyromonas gingivalis in infected root canals and their susceptibility to endodontic treatment procedures: A molecular study, Prevalencija bakterija Enterococcus faecalis i Porphyromonas gingivalis u inficiranim kanalima korena zuba i njihova osetljivost na endodontsko lečenje - molekularna studija",
volume = "142",
number = "9-10",
pages = "535-541",
doi = "10.2298/SARH1410535S"
}

Stojanović, N., Krunić, J., Popović, B., Stojičić, S.,& Živković, S.. (2014). Prevalence of Enterococcus faecalis and Porphyromonas gingivalis in infected root canals and their susceptibility to endodontic treatment procedures: A molecular study. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 142(9-10), 535-541.
https://doi.org/10.2298/SARH1410535S

Stojanović N, Krunić J, Popović B, Stojičić S, Živković S. Prevalence of Enterococcus faecalis and Porphyromonas gingivalis in infected root canals and their susceptibility to endodontic treatment procedures: A molecular study. in Srpski arhiv za celokupno lekarstvo. 2014;142(9-10):535-541.
doi:10.2298/SARH1410535S .

Stojanović, Nikola, Krunić, Jelena, Popović, Branka, Stojičić, Sonja, Živković, Slavoljub, "Prevalence of Enterococcus faecalis and Porphyromonas gingivalis in infected root canals and their susceptibility to endodontic treatment procedures: A molecular study" in Srpski arhiv za celokupno lekarstvo, 142, no. 9-10 (2014):535-541,
https://doi.org/10.2298/SARH1410535S . .

TY  - JOUR
AU  - Jekić, Biljana
AU  - Luković, Ljiljana
AU  - Bunjevački, Vera
AU  - Milić, Vera
AU  - Novaković, Ivana
AU  - Damnjanović, Tatjana
AU  - Milašin, Jelena
AU  - Popović, Branka
AU  - Maksimović, Nela
AU  - Damjanov, Nemanja
AU  - Radunović, Goran
AU  - Kovacević, Ljiljana
AU  - Krajinović, Maja
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1839
AB  - Purpose Gamma-glutamyl hydrolase (GGH), cyclin D1 (CCND1) and thymidylate synthase (TS) genes encode enzymes that are involved in methotrexate (MTX) action. In a group of 184 RA patients treated with MTX, we have investigated whether selected polymorphisms in these genes modulate MTX efficacy and/or have impact on adverse drug effects (ADEs). Methods The efficacy of the MTX therapy has been estimated using the disease activity score in 28 joints (DAS28-ESR) based on EULAR criteria and relative DAS28 values (rDAS28). All adverse drug events were recorded. Patients were genotyped for selected polymorphisms of the GGH (-354 G>T and 452 C>T), CCND1 (870 A>G) and TYMS (variable number of tandem repeats, VNTR, and G to C substitution of triple repeat, 3R allele) gene. Association studies have been performed between obtained genotypes and the efficacy and toxicity of MTX. Results According to the EULAR response criteria, 146 RA patients (79.3 %) were classified as responders (good/moderate response) and 38 (20.7 %) as nonresponders (poor response). Higher frequency of the TYMS 3 G/3 G genotype has been found among non-responders as compared to individuals with remaining genotypes (p=0.02). ADEs were recorded in 53 patients. Among those patients eight experienced bone marrow toxicity, all of them carried GGH -354GG genotype (p=0.003). No other significant association were observed. Conclusion The 3 G/3 G genotype of the TYMS gene may indicate predisposition of poor response to MTX and GG genotype of GGH -354 T>G polymorphism may have high predictive value for myelosuppression in RA patients.
PB  - Springer Heidelberg, Heidelberg
T2  - European Journal of Clinical Pharmacology
T1  - Association of the TYMS 3G/3G genotype with poor response and GGH 354GG genotype with the bone marrow toxicity of the methotrexate in RA patients
VL  - 69
IS  - 3
SP  - 377
EP  - 383
DO  - 10.1007/s00228-012-1341-3
ER  - 

@article{
author = "Jekić, Biljana and Luković, Ljiljana and Bunjevački, Vera and Milić, Vera and Novaković, Ivana and Damnjanović, Tatjana and Milašin, Jelena and Popović, Branka and Maksimović, Nela and Damjanov, Nemanja and Radunović, Goran and Kovacević, Ljiljana and Krajinović, Maja",
year = "2013",
abstract = "Purpose Gamma-glutamyl hydrolase (GGH), cyclin D1 (CCND1) and thymidylate synthase (TS) genes encode enzymes that are involved in methotrexate (MTX) action. In a group of 184 RA patients treated with MTX, we have investigated whether selected polymorphisms in these genes modulate MTX efficacy and/or have impact on adverse drug effects (ADEs). Methods The efficacy of the MTX therapy has been estimated using the disease activity score in 28 joints (DAS28-ESR) based on EULAR criteria and relative DAS28 values (rDAS28). All adverse drug events were recorded. Patients were genotyped for selected polymorphisms of the GGH (-354 G>T and 452 C>T), CCND1 (870 A>G) and TYMS (variable number of tandem repeats, VNTR, and G to C substitution of triple repeat, 3R allele) gene. Association studies have been performed between obtained genotypes and the efficacy and toxicity of MTX. Results According to the EULAR response criteria, 146 RA patients (79.3 %) were classified as responders (good/moderate response) and 38 (20.7 %) as nonresponders (poor response). Higher frequency of the TYMS 3 G/3 G genotype has been found among non-responders as compared to individuals with remaining genotypes (p=0.02). ADEs were recorded in 53 patients. Among those patients eight experienced bone marrow toxicity, all of them carried GGH -354GG genotype (p=0.003). No other significant association were observed. Conclusion The 3 G/3 G genotype of the TYMS gene may indicate predisposition of poor response to MTX and GG genotype of GGH -354 T>G polymorphism may have high predictive value for myelosuppression in RA patients.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "European Journal of Clinical Pharmacology",
title = "Association of the TYMS 3G/3G genotype with poor response and GGH 354GG genotype with the bone marrow toxicity of the methotrexate in RA patients",
volume = "69",
number = "3",
pages = "377-383",
doi = "10.1007/s00228-012-1341-3"
}

Jekić, B., Luković, L., Bunjevački, V., Milić, V., Novaković, I., Damnjanović, T., Milašin, J., Popović, B., Maksimović, N., Damjanov, N., Radunović, G., Kovacević, L.,& Krajinović, M.. (2013). Association of the TYMS 3G/3G genotype with poor response and GGH 354GG genotype with the bone marrow toxicity of the methotrexate in RA patients. in European Journal of Clinical Pharmacology
Springer Heidelberg, Heidelberg., 69(3), 377-383.
https://doi.org/10.1007/s00228-012-1341-3

Jekić B, Luković L, Bunjevački V, Milić V, Novaković I, Damnjanović T, Milašin J, Popović B, Maksimović N, Damjanov N, Radunović G, Kovacević L, Krajinović M. Association of the TYMS 3G/3G genotype with poor response and GGH 354GG genotype with the bone marrow toxicity of the methotrexate in RA patients. in European Journal of Clinical Pharmacology. 2013;69(3):377-383.
doi:10.1007/s00228-012-1341-3 .

Jekić, Biljana, Luković, Ljiljana, Bunjevački, Vera, Milić, Vera, Novaković, Ivana, Damnjanović, Tatjana, Milašin, Jelena, Popović, Branka, Maksimović, Nela, Damjanov, Nemanja, Radunović, Goran, Kovacević, Ljiljana, Krajinović, Maja, "Association of the TYMS 3G/3G genotype with poor response and GGH 354GG genotype with the bone marrow toxicity of the methotrexate in RA patients" in European Journal of Clinical Pharmacology, 69, no. 3 (2013):377-383,
https://doi.org/10.1007/s00228-012-1341-3 . .

TY  - JOUR
AU  - Regueiro, Maria
AU  - Rivera, Luis
AU  - Chennakrishnaiah, Shilpa
AU  - Popović, Branka
AU  - Andjus, Stefan
AU  - Milašin, Jelena
AU  - Herrera, Rene J.
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1763
AB  - One of the primary unanswered questions regarding the dispersal of Romani populations concerns the geographical region and/or the Indian caste/tribe that gave rise to the proto-Romani group. To shed light on this matter, 161 Y-chromosomes from Roma, residing in two different provinces of Serbia, were analyzed. Our results indicate that the paternal gene pool of both groups is shaped by several strata, the most prominent of which, H1-M52, comprises almost half of each collection's patrilineages. The high frequency of M52 chromosomes in the two Roma populations examined may suggest that they descend from a single founder that has its origins in the Indian subcontinent. Moreover, when the Y-STR profiles of haplogroup H derived individuals in our Roma populations were compared to those typed in the South Indian emigrants from Malaysia and groups from Madras, Karnataka (Lingayat and Vokkaliga castes) and tribal Soligas, sharing of the two most common haplotypes was observed. These similarities suggest that South India may have been one of the contributors to the proto-Romanis. European genetic signatures (i.e., haplogroups E1b1b1a1b-V13, G2a-P15, I-M258, J2-M172 and R1-M173), on the other hand, were also detected in both groups, but at varying frequencies. The divergent European genetic signals in each collection are likely the result of differential gene flow and/or admixture with the European host populations but may also be attributed to dissimilar endogamous practices following the initial founder effect. Our data also support the notion that a number of haplogroups including G2a-P15, J2a3b-M67(xM92), I-M258 and E1b1b1-M35 were incorporated into the proto-Romani paternal lineages as migrants moved from northern India through Southwestern Asia, the Middle East and/or Anatolia into the Balkans.
PB  - Elsevier, Amsterdam
T2  - Gene
T1  - Ancestral modal Y-STR haplotype shared among Romani and South Indian populations
VL  - 504
IS  - 2
SP  - 296
EP  - 302
DO  - 10.1016/j.gene.2012.04.093
ER  - 

@article{
author = "Regueiro, Maria and Rivera, Luis and Chennakrishnaiah, Shilpa and Popović, Branka and Andjus, Stefan and Milašin, Jelena and Herrera, Rene J.",
year = "2012",
abstract = "One of the primary unanswered questions regarding the dispersal of Romani populations concerns the geographical region and/or the Indian caste/tribe that gave rise to the proto-Romani group. To shed light on this matter, 161 Y-chromosomes from Roma, residing in two different provinces of Serbia, were analyzed. Our results indicate that the paternal gene pool of both groups is shaped by several strata, the most prominent of which, H1-M52, comprises almost half of each collection's patrilineages. The high frequency of M52 chromosomes in the two Roma populations examined may suggest that they descend from a single founder that has its origins in the Indian subcontinent. Moreover, when the Y-STR profiles of haplogroup H derived individuals in our Roma populations were compared to those typed in the South Indian emigrants from Malaysia and groups from Madras, Karnataka (Lingayat and Vokkaliga castes) and tribal Soligas, sharing of the two most common haplotypes was observed. These similarities suggest that South India may have been one of the contributors to the proto-Romanis. European genetic signatures (i.e., haplogroups E1b1b1a1b-V13, G2a-P15, I-M258, J2-M172 and R1-M173), on the other hand, were also detected in both groups, but at varying frequencies. The divergent European genetic signals in each collection are likely the result of differential gene flow and/or admixture with the European host populations but may also be attributed to dissimilar endogamous practices following the initial founder effect. Our data also support the notion that a number of haplogroups including G2a-P15, J2a3b-M67(xM92), I-M258 and E1b1b1-M35 were incorporated into the proto-Romani paternal lineages as migrants moved from northern India through Southwestern Asia, the Middle East and/or Anatolia into the Balkans.",
publisher = "Elsevier, Amsterdam",
journal = "Gene",
title = "Ancestral modal Y-STR haplotype shared among Romani and South Indian populations",
volume = "504",
number = "2",
pages = "296-302",
doi = "10.1016/j.gene.2012.04.093"
}

Regueiro, M., Rivera, L., Chennakrishnaiah, S., Popović, B., Andjus, S., Milašin, J.,& Herrera, R. J.. (2012). Ancestral modal Y-STR haplotype shared among Romani and South Indian populations. in Gene
Elsevier, Amsterdam., 504(2), 296-302.
https://doi.org/10.1016/j.gene.2012.04.093

Regueiro M, Rivera L, Chennakrishnaiah S, Popović B, Andjus S, Milašin J, Herrera RJ. Ancestral modal Y-STR haplotype shared among Romani and South Indian populations. in Gene. 2012;504(2):296-302.
doi:10.1016/j.gene.2012.04.093 .

Regueiro, Maria, Rivera, Luis, Chennakrishnaiah, Shilpa, Popović, Branka, Andjus, Stefan, Milašin, Jelena, Herrera, Rene J., "Ancestral modal Y-STR haplotype shared among Romani and South Indian populations" in Gene, 504, no. 2 (2012):296-302,
https://doi.org/10.1016/j.gene.2012.04.093 . .

TY  - JOUR
AU  - Milić, Vera
AU  - Jekić, Biljana
AU  - Luković, Ljiljana
AU  - Bunjevački, Vera
AU  - Milašin, Jelena
AU  - Novaković, I.
AU  - Damnjanović, Tatjana
AU  - Popović, Branka
AU  - Maksimović, Nela
AU  - Damjanov, Nemanja
AU  - Radunović, Goran
AU  - Pejnović, Nada
AU  - Krajinović, Maja
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1741
AB  - Objectives Identifying genetic predictors of methotrexate (MTX) treatment response in patients with rheumatoid arthritis (RA) may have great importance for optimising drug doses required for clinical benefit without toxicity. In a group of 125 RA patients treated with MTX we investigated whether selected polymorphisms in genes relevant for MTX action (aminoimidazole-4-carboxiamide ribonucleotide transformylase, ATIC, and dihydrofolate reductase, DHFR) modulate disease activity and/or have impact on therapy side effects. Methods The efficacy of treatment was estimated both by the disease activity score in 28 joints (DAS28), based on EULAR criteria, and relative DAS28 (rDAS28) score. Adverse drug events (ADEs) were also recorded. RA patients were genotyped using the PCR-RFLP method, followed by an association study between ATIC -129T>G, DHFR -216T>C and DHFR -317A>G polymorphisms and the efficacy and toxicity of MTX. Results According to the EULAR response criteria, 96 RA patients (76.8%) were classified as responders (good/moderate response) and 29 (23.2%) as non-responders (poor response). rDAS28 values ranged from -0.01 to 0.80 (mean value 0.31 +/- 0.19). Among 125 patients enrolled in this study 39 experienced at least one side effect. The DHFR -317AA genotype was associated with the less favourable response (reduction in rDAS28 score, p=0.05). None of the analysed polymorphisms was associated with MTX toxicity. Conclusion RA patients with DHFR-317AA genotype had less favourable response to MTX Further studies in larger patient populations are necessary to confirm the relationship between the analysed polymorphisms and MTX treatment response.
PB  - Clinical & Exper Rheumatology, Pisa
T2  - Clinical & Experimental Rheumatology
T1  - Association of dihydrofolate reductase (DHFR)-317AA genotype with poor response to methotrexate in patients with rheumatoid arthritis
VL  - 30
IS  - 2
SP  - 178
EP  - 183
UR  - https://hdl.handle.net/21.15107/rcub_smile_1741
ER  - 

@article{
author = "Milić, Vera and Jekić, Biljana and Luković, Ljiljana and Bunjevački, Vera and Milašin, Jelena and Novaković, I. and Damnjanović, Tatjana and Popović, Branka and Maksimović, Nela and Damjanov, Nemanja and Radunović, Goran and Pejnović, Nada and Krajinović, Maja",
year = "2012",
abstract = "Objectives Identifying genetic predictors of methotrexate (MTX) treatment response in patients with rheumatoid arthritis (RA) may have great importance for optimising drug doses required for clinical benefit without toxicity. In a group of 125 RA patients treated with MTX we investigated whether selected polymorphisms in genes relevant for MTX action (aminoimidazole-4-carboxiamide ribonucleotide transformylase, ATIC, and dihydrofolate reductase, DHFR) modulate disease activity and/or have impact on therapy side effects. Methods The efficacy of treatment was estimated both by the disease activity score in 28 joints (DAS28), based on EULAR criteria, and relative DAS28 (rDAS28) score. Adverse drug events (ADEs) were also recorded. RA patients were genotyped using the PCR-RFLP method, followed by an association study between ATIC -129T>G, DHFR -216T>C and DHFR -317A>G polymorphisms and the efficacy and toxicity of MTX. Results According to the EULAR response criteria, 96 RA patients (76.8%) were classified as responders (good/moderate response) and 29 (23.2%) as non-responders (poor response). rDAS28 values ranged from -0.01 to 0.80 (mean value 0.31 +/- 0.19). Among 125 patients enrolled in this study 39 experienced at least one side effect. The DHFR -317AA genotype was associated with the less favourable response (reduction in rDAS28 score, p=0.05). None of the analysed polymorphisms was associated with MTX toxicity. Conclusion RA patients with DHFR-317AA genotype had less favourable response to MTX Further studies in larger patient populations are necessary to confirm the relationship between the analysed polymorphisms and MTX treatment response.",
publisher = "Clinical & Exper Rheumatology, Pisa",
journal = "Clinical & Experimental Rheumatology",
title = "Association of dihydrofolate reductase (DHFR)-317AA genotype with poor response to methotrexate in patients with rheumatoid arthritis",
volume = "30",
number = "2",
pages = "178-183",
url = "https://hdl.handle.net/21.15107/rcub_smile_1741"
}

Milić, V., Jekić, B., Luković, L., Bunjevački, V., Milašin, J., Novaković, I., Damnjanović, T., Popović, B., Maksimović, N., Damjanov, N., Radunović, G., Pejnović, N.,& Krajinović, M.. (2012). Association of dihydrofolate reductase (DHFR)-317AA genotype with poor response to methotrexate in patients with rheumatoid arthritis. in Clinical & Experimental Rheumatology
Clinical & Exper Rheumatology, Pisa., 30(2), 178-183.
https://hdl.handle.net/21.15107/rcub_smile_1741

Milić V, Jekić B, Luković L, Bunjevački V, Milašin J, Novaković I, Damnjanović T, Popović B, Maksimović N, Damjanov N, Radunović G, Pejnović N, Krajinović M. Association of dihydrofolate reductase (DHFR)-317AA genotype with poor response to methotrexate in patients with rheumatoid arthritis. in Clinical & Experimental Rheumatology. 2012;30(2):178-183.
https://hdl.handle.net/21.15107/rcub_smile_1741 .

Milić, Vera, Jekić, Biljana, Luković, Ljiljana, Bunjevački, Vera, Milašin, Jelena, Novaković, I., Damnjanović, Tatjana, Popović, Branka, Maksimović, Nela, Damjanov, Nemanja, Radunović, Goran, Pejnović, Nada, Krajinović, Maja, "Association of dihydrofolate reductase (DHFR)-317AA genotype with poor response to methotrexate in patients with rheumatoid arthritis" in Clinical & Experimental Rheumatology, 30, no. 2 (2012):178-183,
https://hdl.handle.net/21.15107/rcub_smile_1741 .

TY  - JOUR
AU  - Puzović, Dragana
AU  - Dunjić, D.
AU  - Popović, Branka
AU  - Stojković, O.
AU  - Novaković, Ivana
AU  - Milašin, Jelena
PY  - 2011
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1637
AB  - Dentin provides a protective enclosure for genomic and mitochondrial DNA. In the present study, DNA was obtained from pulverized or ground teeth. The quality of the DNA extracted from the teeth of 70 unrelated individuals was tested in the context of assessing the allelic and genotypic frequencies of autosomal loci D19S216, D20S502 and D20S842, and calculating a number of parameters of population genetics and forensic interest. This study illustrates that teeth can be a convenient tissue to extract DNA from large numbers of individuals for population genetic studies as well as for forensic case work.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - STR loci D19S216, D20S502 and D20S842 analysis in the Serbian population using dentin DNA
VL  - 63
IS  - 1
SP  - 55
EP  - 58
DO  - 10.2298/ABS1101055P
ER  - 

@article{
author = "Puzović, Dragana and Dunjić, D. and Popović, Branka and Stojković, O. and Novaković, Ivana and Milašin, Jelena",
year = "2011",
abstract = "Dentin provides a protective enclosure for genomic and mitochondrial DNA. In the present study, DNA was obtained from pulverized or ground teeth. The quality of the DNA extracted from the teeth of 70 unrelated individuals was tested in the context of assessing the allelic and genotypic frequencies of autosomal loci D19S216, D20S502 and D20S842, and calculating a number of parameters of population genetics and forensic interest. This study illustrates that teeth can be a convenient tissue to extract DNA from large numbers of individuals for population genetic studies as well as for forensic case work.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "STR loci D19S216, D20S502 and D20S842 analysis in the Serbian population using dentin DNA",
volume = "63",
number = "1",
pages = "55-58",
doi = "10.2298/ABS1101055P"
}

Puzović, D., Dunjić, D., Popović, B., Stojković, O., Novaković, I.,& Milašin, J.. (2011). STR loci D19S216, D20S502 and D20S842 analysis in the Serbian population using dentin DNA. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(1), 55-58.
https://doi.org/10.2298/ABS1101055P

Puzović D, Dunjić D, Popović B, Stojković O, Novaković I, Milašin J. STR loci D19S216, D20S502 and D20S842 analysis in the Serbian population using dentin DNA. in Archives of Biological Sciences. 2011;63(1):55-58.
doi:10.2298/ABS1101055P .

Puzović, Dragana, Dunjić, D., Popović, Branka, Stojković, O., Novaković, Ivana, Milašin, Jelena, "STR loci D19S216, D20S502 and D20S842 analysis in the Serbian population using dentin DNA" in Archives of Biological Sciences, 63, no. 1 (2011):55-58,
https://doi.org/10.2298/ABS1101055P . .

TY  - JOUR
AU  - Jekić, Biljana
AU  - Bunjevački, Vera
AU  - Dobričić, Valerija
AU  - Novaković, Ivana
AU  - Milašin, Jelena
AU  - Popović, Branka
AU  - Damnjanović, Tatjana
AU  - Maksimović, Nela
AU  - Perović, V.
AU  - Luković, Ljiljana
PY  - 2011
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1635
AB  - Myelodysplastic syndromes (MDS) are rare in children and only a few studies have analyzed their molecular mechanisms. The NPM1 gene encodes for nucleophosmin (NPM) which regulates hematopoiesis. Mutations in exon 12 of the NPM1 cause the nucleophosmin cytoplasmic dislocation and disrupt its functions. We have analyzed mutations of the NPM1 gene in archival bone marrow samples from 17 children with MDS and detected, in one patient, transition C to T in codon 293. To the best of our knowledge, this is the first analysis of NPM1 mutations in childhood MDS and the very first missense mutation of the NPM1 gene reported so far.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - NPM1 gene mutations in children with Myelodysplastic syndromes
VL  - 63
IS  - 3
SP  - 649
EP  - 653
DO  - 10.2298/ABS1103649J
ER  - 

@article{
author = "Jekić, Biljana and Bunjevački, Vera and Dobričić, Valerija and Novaković, Ivana and Milašin, Jelena and Popović, Branka and Damnjanović, Tatjana and Maksimović, Nela and Perović, V. and Luković, Ljiljana",
year = "2011",
abstract = "Myelodysplastic syndromes (MDS) are rare in children and only a few studies have analyzed their molecular mechanisms. The NPM1 gene encodes for nucleophosmin (NPM) which regulates hematopoiesis. Mutations in exon 12 of the NPM1 cause the nucleophosmin cytoplasmic dislocation and disrupt its functions. We have analyzed mutations of the NPM1 gene in archival bone marrow samples from 17 children with MDS and detected, in one patient, transition C to T in codon 293. To the best of our knowledge, this is the first analysis of NPM1 mutations in childhood MDS and the very first missense mutation of the NPM1 gene reported so far.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "NPM1 gene mutations in children with Myelodysplastic syndromes",
volume = "63",
number = "3",
pages = "649-653",
doi = "10.2298/ABS1103649J"
}

Jekić, B., Bunjevački, V., Dobričić, V., Novaković, I., Milašin, J., Popović, B., Damnjanović, T., Maksimović, N., Perović, V.,& Luković, L.. (2011). NPM1 gene mutations in children with Myelodysplastic syndromes. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(3), 649-653.
https://doi.org/10.2298/ABS1103649J

Jekić B, Bunjevački V, Dobričić V, Novaković I, Milašin J, Popović B, Damnjanović T, Maksimović N, Perović V, Luković L. NPM1 gene mutations in children with Myelodysplastic syndromes. in Archives of Biological Sciences. 2011;63(3):649-653.
doi:10.2298/ABS1103649J .

Jekić, Biljana, Bunjevački, Vera, Dobričić, Valerija, Novaković, Ivana, Milašin, Jelena, Popović, Branka, Damnjanović, Tatjana, Maksimović, Nela, Perović, V., Luković, Ljiljana, "NPM1 gene mutations in children with Myelodysplastic syndromes" in Archives of Biological Sciences, 63, no. 3 (2011):649-653,
https://doi.org/10.2298/ABS1103649J . .

TY  - JOUR
AU  - Andrić, Miroslav
AU  - Dožić, Branko
AU  - Popović, Branka
AU  - Stefanović, D.
AU  - Basta-Jovanović, Gordana
AU  - Đogo, N.
AU  - Andjus, P.
AU  - Milašin, Jelena
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1531
AB  - Objectives: The aim of this study was to investigate the expression of survivin, an inhibitor of apoptosis, in odontogenic keratocysts and to compare it to the findings in non-neoplastic jaw cysts - periapical cysts, as well as to establish a possible relationship between survivin expression and human cytomegalovirus presence within these cysts. Materials and methods: Samples of 10 odontogenic keratocysts (five positive and five negative for the presence of cytomegalovirus, as determined by polymerase chain reaction) and 10 periapical cysts (five positive and five negative for the cytomegalovirus presence) were analysed. The expression of survivin was assessed by immunohistochemical methods, using monoclonal antibody that selectively recognizes the cytoplasmic form of survivin. Results: All 10 odontogenic keratocysts showed immunostaining for survivin, while all 10 periapical cysts were negative for its presence. There was no correlation between cytomegalovirus presence and expression of survivin within odontogenic keratocysts. Conclusion: Survivin may contribute to the aggressive behavior of odontogenic keratocysts, and thus support the emerging opinion of their neoplastic nature.
PB  - Wiley, Hoboken
T2  - Oral Diseases
T1  - Survivin expression in odontogenic keratocysts and correlation with cytomegalovirus infection
VL  - 16
IS  - 2
SP  - 156
EP  - 159
DO  - 10.1111/j.1601-0825.2009.01612.x
ER  - 

@article{
author = "Andrić, Miroslav and Dožić, Branko and Popović, Branka and Stefanović, D. and Basta-Jovanović, Gordana and Đogo, N. and Andjus, P. and Milašin, Jelena",
year = "2010",
abstract = "Objectives: The aim of this study was to investigate the expression of survivin, an inhibitor of apoptosis, in odontogenic keratocysts and to compare it to the findings in non-neoplastic jaw cysts - periapical cysts, as well as to establish a possible relationship between survivin expression and human cytomegalovirus presence within these cysts. Materials and methods: Samples of 10 odontogenic keratocysts (five positive and five negative for the presence of cytomegalovirus, as determined by polymerase chain reaction) and 10 periapical cysts (five positive and five negative for the cytomegalovirus presence) were analysed. The expression of survivin was assessed by immunohistochemical methods, using monoclonal antibody that selectively recognizes the cytoplasmic form of survivin. Results: All 10 odontogenic keratocysts showed immunostaining for survivin, while all 10 periapical cysts were negative for its presence. There was no correlation between cytomegalovirus presence and expression of survivin within odontogenic keratocysts. Conclusion: Survivin may contribute to the aggressive behavior of odontogenic keratocysts, and thus support the emerging opinion of their neoplastic nature.",
publisher = "Wiley, Hoboken",
journal = "Oral Diseases",
title = "Survivin expression in odontogenic keratocysts and correlation with cytomegalovirus infection",
volume = "16",
number = "2",
pages = "156-159",
doi = "10.1111/j.1601-0825.2009.01612.x"
}

Andrić, M., Dožić, B., Popović, B., Stefanović, D., Basta-Jovanović, G., Đogo, N., Andjus, P.,& Milašin, J.. (2010). Survivin expression in odontogenic keratocysts and correlation with cytomegalovirus infection. in Oral Diseases
Wiley, Hoboken., 16(2), 156-159.
https://doi.org/10.1111/j.1601-0825.2009.01612.x

Andrić M, Dožić B, Popović B, Stefanović D, Basta-Jovanović G, Đogo N, Andjus P, Milašin J. Survivin expression in odontogenic keratocysts and correlation with cytomegalovirus infection. in Oral Diseases. 2010;16(2):156-159.
doi:10.1111/j.1601-0825.2009.01612.x .

Andrić, Miroslav, Dožić, Branko, Popović, Branka, Stefanović, D., Basta-Jovanović, Gordana, Đogo, N., Andjus, P., Milašin, Jelena, "Survivin expression in odontogenic keratocysts and correlation with cytomegalovirus infection" in Oral Diseases, 16, no. 2 (2010):156-159,
https://doi.org/10.1111/j.1601-0825.2009.01612.x . .

TY  - JOUR
AU  - Popović, Branka
AU  - Jekić, Biljana
AU  - Novaković, Ivana
AU  - Luković, Ljiljana
AU  - Konstantinović, Vitomir
AU  - Babić, Marko
AU  - Milašin, Jelena
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1564
AB  - The aim of this study was to gain a better understanding of cancer genes contributing to oral squamous cell (OSCC) development and progression and correlate genetic changes to clinical parameters. Human papilloma virus (HPV) 16 detection is also included in the study. 60 samples of OSCC were analysed for erbB2 and c-myc amplification by dPCR, H-ras and p53 point mutations by PCR/SSCP. HPV was detected via amplification of its El and E6 genes. c-erbB2 was altered in 45%, c-myc in 35%, H-ras in 22% and p53 in 60% of samples. HPV was detected in 10% of cases. The frequency of p53 gene mutations showed a statistically significant association with tumour stage. Patients with c-erbB2 and H-ras alterations had lower survival than patients without these alterations. The number of detected genetic changes was remarkable but statistical association with tumour natural history was poor, indicating high clonal heterogeneity and multiple pathways of carcinogenesis.
PB  - Churchill Livingstone, Edinburgh
T2  - International Journal of Oral & Maxillofacial Surgery
T1  - Cancer genes alterations and HPV infection in oral squamous cell carcinoma
VL  - 39
IS  - 9
SP  - 909
EP  - 915
DO  - 10.1016/j.ijom.2010.05.007
ER  - 

@article{
author = "Popović, Branka and Jekić, Biljana and Novaković, Ivana and Luković, Ljiljana and Konstantinović, Vitomir and Babić, Marko and Milašin, Jelena",
year = "2010",
abstract = "The aim of this study was to gain a better understanding of cancer genes contributing to oral squamous cell (OSCC) development and progression and correlate genetic changes to clinical parameters. Human papilloma virus (HPV) 16 detection is also included in the study. 60 samples of OSCC were analysed for erbB2 and c-myc amplification by dPCR, H-ras and p53 point mutations by PCR/SSCP. HPV was detected via amplification of its El and E6 genes. c-erbB2 was altered in 45%, c-myc in 35%, H-ras in 22% and p53 in 60% of samples. HPV was detected in 10% of cases. The frequency of p53 gene mutations showed a statistically significant association with tumour stage. Patients with c-erbB2 and H-ras alterations had lower survival than patients without these alterations. The number of detected genetic changes was remarkable but statistical association with tumour natural history was poor, indicating high clonal heterogeneity and multiple pathways of carcinogenesis.",
publisher = "Churchill Livingstone, Edinburgh",
journal = "International Journal of Oral & Maxillofacial Surgery",
title = "Cancer genes alterations and HPV infection in oral squamous cell carcinoma",
volume = "39",
number = "9",
pages = "909-915",
doi = "10.1016/j.ijom.2010.05.007"
}

Popović, B., Jekić, B., Novaković, I., Luković, L., Konstantinović, V., Babić, M.,& Milašin, J.. (2010). Cancer genes alterations and HPV infection in oral squamous cell carcinoma. in International Journal of Oral & Maxillofacial Surgery
Churchill Livingstone, Edinburgh., 39(9), 909-915.
https://doi.org/10.1016/j.ijom.2010.05.007

Popović B, Jekić B, Novaković I, Luković L, Konstantinović V, Babić M, Milašin J. Cancer genes alterations and HPV infection in oral squamous cell carcinoma. in International Journal of Oral & Maxillofacial Surgery. 2010;39(9):909-915.
doi:10.1016/j.ijom.2010.05.007 .

Popović, Branka, Jekić, Biljana, Novaković, Ivana, Luković, Ljiljana, Konstantinović, Vitomir, Babić, Marko, Milašin, Jelena, "Cancer genes alterations and HPV infection in oral squamous cell carcinoma" in International Journal of Oral & Maxillofacial Surgery, 39, no. 9 (2010):909-915,
https://doi.org/10.1016/j.ijom.2010.05.007 . .

TY  - JOUR
AU  - Popović, Branka
AU  - Jekić, Biljana
AU  - Jelovac, Drago
AU  - Novaković, Ivana
PY  - 2009
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1470
AB  - Introduction. p53 gene is the most common tumor suppressor gene involved in pathogenesis oral squamous cell carcinoma (OSCC). Protein product of p53 gene contributes to cell cycle control and apoptosis. p53 gene mutations may lead to uncontrolled cell growth. The aim of this study was to determine the incidence of mutation in DNA-binding domain of p53 gene. Materials and Methods. In the 60 specimens, the presence of point mutation in exons 5, 6, 7 and 8 was detected using PCR-SSCP method. To confirm the presence of p53 mutation found by SSCP method, five samples were analyzed by sequencing of exon 5. Results. Point mutation affecting exons 5, 6, 7 and 8 were found in 60% of analyzed samples. A higher incidence of mutation was detected in exon 7 and 8 (60%), than in exon 5 and 6. Sequencing of exon 5, confirmed the presence of mutations revealed by SSCP method. Study of associations showed an increase of p53 mutations in poor differentiated and carcinoma of higher clinical stages. Conclusion. p53 gene is one of major factor in control of cell cycle and has important role in pathogenesis of oral squamous cell carcinoma.
AB  - Uvod. TP53 je ključni tumor-supresorski gen uključen u patogenezu oralnih skvamocelularnih karcinoma (OSCK). Proteinski proizvod gena p53 zadužen je za kontrolu ćelijskog ciklusa i apoptozu, a mutacije u TP53 mogu dovesti do nekontrolisane proliferacije ćelija. Cilj ovog rada je bio da se utvrdi zastupljenost tačkastih mutacija u regionu gena p53 koji se vezuje za DNK, odnosno proceni uloga ovoga gena u patogenezi OSCK. Materijal i metode rada. U 60 uzoraka OSCK su korišćenjem metode lančane reakcije polimeraze i polimorfizma jednolančanih fragmenata DNK (PCR-SSCP) ispitane tačkaste mutacije u egzonima 5, 6, 7 i 8 gena p53. Pet slučajno odabranih uzoraka u kojima je otkrivena mutacija naknadno je podvrgnuto sekvenciranju radi potvrde validnosti metode PCR-SSCP. Rezultati. Tačkaste mutacije u nekom od analiziranih egzona gena p53 utvrđene su u 60% uzoraka OSCK. Veća učestalost mutacija zabeležena je u egzonima 7 i 8. Sekvenciranje je potvrdilo mutacije otkrivene metodom SSCP. Studija asocijacije pokazuje povećanje broja mutacija gena p53 kod slabo diferenciranih i karcinoma viših kliničkih stadijuma. Zaključak. Gen p53, jedan od glavnih kontrolora ćelijskog ciklusa, ima značajnu ulogu i u patogenezi karcinoma oralne duplje.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Mutation status of p53 gene in oral squamous cell carcinoma
T1  - Mutacioni status gena p53 u oralnim skvamocelularnim karcinomima
VL  - 56
IS  - 4
SP  - 171
EP  - 175
DO  - 10.2298/SGS0904171P
ER  - 

@article{
author = "Popović, Branka and Jekić, Biljana and Jelovac, Drago and Novaković, Ivana",
year = "2009",
abstract = "Introduction. p53 gene is the most common tumor suppressor gene involved in pathogenesis oral squamous cell carcinoma (OSCC). Protein product of p53 gene contributes to cell cycle control and apoptosis. p53 gene mutations may lead to uncontrolled cell growth. The aim of this study was to determine the incidence of mutation in DNA-binding domain of p53 gene. Materials and Methods. In the 60 specimens, the presence of point mutation in exons 5, 6, 7 and 8 was detected using PCR-SSCP method. To confirm the presence of p53 mutation found by SSCP method, five samples were analyzed by sequencing of exon 5. Results. Point mutation affecting exons 5, 6, 7 and 8 were found in 60% of analyzed samples. A higher incidence of mutation was detected in exon 7 and 8 (60%), than in exon 5 and 6. Sequencing of exon 5, confirmed the presence of mutations revealed by SSCP method. Study of associations showed an increase of p53 mutations in poor differentiated and carcinoma of higher clinical stages. Conclusion. p53 gene is one of major factor in control of cell cycle and has important role in pathogenesis of oral squamous cell carcinoma., Uvod. TP53 je ključni tumor-supresorski gen uključen u patogenezu oralnih skvamocelularnih karcinoma (OSCK). Proteinski proizvod gena p53 zadužen je za kontrolu ćelijskog ciklusa i apoptozu, a mutacije u TP53 mogu dovesti do nekontrolisane proliferacije ćelija. Cilj ovog rada je bio da se utvrdi zastupljenost tačkastih mutacija u regionu gena p53 koji se vezuje za DNK, odnosno proceni uloga ovoga gena u patogenezi OSCK. Materijal i metode rada. U 60 uzoraka OSCK su korišćenjem metode lančane reakcije polimeraze i polimorfizma jednolančanih fragmenata DNK (PCR-SSCP) ispitane tačkaste mutacije u egzonima 5, 6, 7 i 8 gena p53. Pet slučajno odabranih uzoraka u kojima je otkrivena mutacija naknadno je podvrgnuto sekvenciranju radi potvrde validnosti metode PCR-SSCP. Rezultati. Tačkaste mutacije u nekom od analiziranih egzona gena p53 utvrđene su u 60% uzoraka OSCK. Veća učestalost mutacija zabeležena je u egzonima 7 i 8. Sekvenciranje je potvrdilo mutacije otkrivene metodom SSCP. Studija asocijacije pokazuje povećanje broja mutacija gena p53 kod slabo diferenciranih i karcinoma viših kliničkih stadijuma. Zaključak. Gen p53, jedan od glavnih kontrolora ćelijskog ciklusa, ima značajnu ulogu i u patogenezi karcinoma oralne duplje.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Mutation status of p53 gene in oral squamous cell carcinoma, Mutacioni status gena p53 u oralnim skvamocelularnim karcinomima",
volume = "56",
number = "4",
pages = "171-175",
doi = "10.2298/SGS0904171P"
}

Popović, B., Jekić, B., Jelovac, D.,& Novaković, I.. (2009). Mutation status of p53 gene in oral squamous cell carcinoma. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 56(4), 171-175.
https://doi.org/10.2298/SGS0904171P

Popović B, Jekić B, Jelovac D, Novaković I. Mutation status of p53 gene in oral squamous cell carcinoma. in Stomatološki glasnik Srbije. 2009;56(4):171-175.
doi:10.2298/SGS0904171P .

Popović, Branka, Jekić, Biljana, Jelovac, Drago, Novaković, Ivana, "Mutation status of p53 gene in oral squamous cell carcinoma" in Stomatološki glasnik Srbije, 56, no. 4 (2009):171-175,
https://doi.org/10.2298/SGS0904171P . .