Radulović, Siniša

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  • Radulović, Siniša (3)
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Author's Bibliography

Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils

Nikolić, Miloš; Jovanović, Katarina K.; Marković, Tatjana; Marković, Dejan; Gligorijević, Nevenka; Radulović, Siniša; Kostić, Marina; Glamočlija, Jasmina; Soković, Marina

(Wiley, Hoboken, 2017)

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Jovanović, Katarina K.
AU  - Marković, Tatjana
AU  - Marković, Dejan
AU  - Gligorijević, Nevenka
AU  - Radulović, Siniša
AU  - Kostić, Marina
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2227
AB  - Objectives The chemical composition, antimicrobial and synergistic effect, and cytotoxic activity of Citrus limon (lemon), Piper nigrum (green pepper) and Melaleuca alternifoila (tea tree) essential oils (EOs) were investigated. Methods Chemical analyses of essential oils were tested by GC-FID and GC-MS spectroscopy. The antimicrobial activity assay was conducted using microdilution method against several oral bacteria and Candida spp. originating from the humans with oral disorders. The synergistic antimicrobial activity was evaluated using checkerboard method. The cytotoxicity evaluation of EOs was assessed using MTT test. Key findings Limonene (37.5%) and beta-pinene (17.9%) were the major compounds in C. limon oil, -pinene (34.4%), delta-3-carene (19.7%), limonene (18.7%) and alpha-pinene (10.4%) in P. nigrum oil and terpinen-4-ol (38.6%) and gamma-terpinene (21.7%) in M. alternifolia oil. The broad-spectrum antimicrobial activity was achieved by tested three EOs, with C. limon oil being the strongest against bacteria and M. alternifolia oil strongest against fungi. The EOs demonstrated synergism; their combined application revealed an increase in antimicrobial activity. All tested essential oils showed lower cytotoxic activity in comparison with the positive control, and the obtained results confirmed a dose-dependent activity. Conclusions The results of this study encourage use of tested EOs in development of a novel agent intended for prevention or therapy of corresponding oral disorders.
PB  - Wiley, Hoboken
T2  - Journal of Pharmacy & Pharmacology
T1  - Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils
VL  - 69
IS  - 11
SP  - 1606
EP  - 1614
DO  - 10.1111/jphp.12792
ER  - 
@article{
author = "Nikolić, Miloš and Jovanović, Katarina K. and Marković, Tatjana and Marković, Dejan and Gligorijević, Nevenka and Radulović, Siniša and Kostić, Marina and Glamočlija, Jasmina and Soković, Marina",
year = "2017",
abstract = "Objectives The chemical composition, antimicrobial and synergistic effect, and cytotoxic activity of Citrus limon (lemon), Piper nigrum (green pepper) and Melaleuca alternifoila (tea tree) essential oils (EOs) were investigated. Methods Chemical analyses of essential oils were tested by GC-FID and GC-MS spectroscopy. The antimicrobial activity assay was conducted using microdilution method against several oral bacteria and Candida spp. originating from the humans with oral disorders. The synergistic antimicrobial activity was evaluated using checkerboard method. The cytotoxicity evaluation of EOs was assessed using MTT test. Key findings Limonene (37.5%) and beta-pinene (17.9%) were the major compounds in C. limon oil, -pinene (34.4%), delta-3-carene (19.7%), limonene (18.7%) and alpha-pinene (10.4%) in P. nigrum oil and terpinen-4-ol (38.6%) and gamma-terpinene (21.7%) in M. alternifolia oil. The broad-spectrum antimicrobial activity was achieved by tested three EOs, with C. limon oil being the strongest against bacteria and M. alternifolia oil strongest against fungi. The EOs demonstrated synergism; their combined application revealed an increase in antimicrobial activity. All tested essential oils showed lower cytotoxic activity in comparison with the positive control, and the obtained results confirmed a dose-dependent activity. Conclusions The results of this study encourage use of tested EOs in development of a novel agent intended for prevention or therapy of corresponding oral disorders.",
publisher = "Wiley, Hoboken",
journal = "Journal of Pharmacy & Pharmacology",
title = "Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils",
volume = "69",
number = "11",
pages = "1606-1614",
doi = "10.1111/jphp.12792"
}
Nikolić, M., Jovanović, K. K., Marković, T., Marković, D., Gligorijević, N., Radulović, S., Kostić, M., Glamočlija, J.,& Soković, M.. (2017). Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils. in Journal of Pharmacy & Pharmacology
Wiley, Hoboken., 69(11), 1606-1614.
https://doi.org/10.1111/jphp.12792
Nikolić M, Jovanović KK, Marković T, Marković D, Gligorijević N, Radulović S, Kostić M, Glamočlija J, Soković M. Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils. in Journal of Pharmacy & Pharmacology. 2017;69(11):1606-1614.
doi:10.1111/jphp.12792 .
Nikolić, Miloš, Jovanović, Katarina K., Marković, Tatjana, Marković, Dejan, Gligorijević, Nevenka, Radulović, Siniša, Kostić, Marina, Glamočlija, Jasmina, Soković, Marina, "Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils" in Journal of Pharmacy & Pharmacology, 69, no. 11 (2017):1606-1614,
https://doi.org/10.1111/jphp.12792 . .
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Chemical composition, antimicrobial, and cytotoxic properties of five Lamiaceae essential oils

Nikolić, Miloš; Jovanović, Katarina K.; Marković, Tatjana; Marković, Dejan; Gligorijević, Nevenka; Radulović, Siniša; Soković, Marina

(Elsevier Science Bv, Amsterdam, 2014)

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Jovanović, Katarina K.
AU  - Marković, Tatjana
AU  - Marković, Dejan
AU  - Gligorijević, Nevenka
AU  - Radulović, Siniša
AU  - Soković, Marina
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1882
AB  - The aim of this study was to assess the biological activity of essential oils from five Lamiaceae species, Mentha piperita, Mentha pulegium, Lavandula angustifolia, Satureja montana and Salvia lavandulifolia, for their chemical composition, antimicrobial and cytotoxic properties. Gas chromatography coupled with FID and MS of essential oils revealed menthol (47.5%), pulegone (68.7), linalool (40.3%), thymol (44.6%), and camphor (29.1%) to be major oils' components, respectively. Minimum inhibitory (MIC) and minimum bactericidal/fungicidal (MBC/MFC) concentrations were determined by microdilution method. Seven bacterial species, representing clinical specimens, including Streptococcus mutans, Streptococcus sanguis, Streptococcus salivarius, Streptococcus pyogenes, Pseudomonas aeruginosa, Lactobacillus acidophilus, and Enterecoccus feacalis, and fifty eight clinical oral Candida spp. isolates along with three reference strains were used in experiment. All essential oils (EOs) exhibited a significant antimicrobial activity against all tested microorganisms; the oil of S. montana proved to be the most potent one (MIC 30.0-630.0 mu g/mL, MBC 60.0-250.0 mu g/mL; MIC 0.9-1.0 mu g/mL; MFC 1.0-3.0 mu g/mL). In addition, the oil also revealed the highest cytotoxic activity against tested cell lines, presenting IC50 values from 40.13 to 65.51 mu g/mL, with mild selectivity towards HeLa cells observed in regard to the normal cell line (MRC-5). In addition to the herbs traditional use in food and pharmacy, results of this study proved the great potential of their essential oils for application in oral disease and anticancer treatments.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops & Products
T1  - Chemical composition, antimicrobial, and cytotoxic properties of five Lamiaceae essential oils
VL  - 61
SP  - 225
EP  - 232
DO  - 10.1016/j.indcrop.2014.07.011
ER  - 
@article{
author = "Nikolić, Miloš and Jovanović, Katarina K. and Marković, Tatjana and Marković, Dejan and Gligorijević, Nevenka and Radulović, Siniša and Soković, Marina",
year = "2014",
abstract = "The aim of this study was to assess the biological activity of essential oils from five Lamiaceae species, Mentha piperita, Mentha pulegium, Lavandula angustifolia, Satureja montana and Salvia lavandulifolia, for their chemical composition, antimicrobial and cytotoxic properties. Gas chromatography coupled with FID and MS of essential oils revealed menthol (47.5%), pulegone (68.7), linalool (40.3%), thymol (44.6%), and camphor (29.1%) to be major oils' components, respectively. Minimum inhibitory (MIC) and minimum bactericidal/fungicidal (MBC/MFC) concentrations were determined by microdilution method. Seven bacterial species, representing clinical specimens, including Streptococcus mutans, Streptococcus sanguis, Streptococcus salivarius, Streptococcus pyogenes, Pseudomonas aeruginosa, Lactobacillus acidophilus, and Enterecoccus feacalis, and fifty eight clinical oral Candida spp. isolates along with three reference strains were used in experiment. All essential oils (EOs) exhibited a significant antimicrobial activity against all tested microorganisms; the oil of S. montana proved to be the most potent one (MIC 30.0-630.0 mu g/mL, MBC 60.0-250.0 mu g/mL; MIC 0.9-1.0 mu g/mL; MFC 1.0-3.0 mu g/mL). In addition, the oil also revealed the highest cytotoxic activity against tested cell lines, presenting IC50 values from 40.13 to 65.51 mu g/mL, with mild selectivity towards HeLa cells observed in regard to the normal cell line (MRC-5). In addition to the herbs traditional use in food and pharmacy, results of this study proved the great potential of their essential oils for application in oral disease and anticancer treatments.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops & Products",
title = "Chemical composition, antimicrobial, and cytotoxic properties of five Lamiaceae essential oils",
volume = "61",
pages = "225-232",
doi = "10.1016/j.indcrop.2014.07.011"
}
Nikolić, M., Jovanović, K. K., Marković, T., Marković, D., Gligorijević, N., Radulović, S.,& Soković, M.. (2014). Chemical composition, antimicrobial, and cytotoxic properties of five Lamiaceae essential oils. in Industrial Crops & Products
Elsevier Science Bv, Amsterdam., 61, 225-232.
https://doi.org/10.1016/j.indcrop.2014.07.011
Nikolić M, Jovanović KK, Marković T, Marković D, Gligorijević N, Radulović S, Soković M. Chemical composition, antimicrobial, and cytotoxic properties of five Lamiaceae essential oils. in Industrial Crops & Products. 2014;61:225-232.
doi:10.1016/j.indcrop.2014.07.011 .
Nikolić, Miloš, Jovanović, Katarina K., Marković, Tatjana, Marković, Dejan, Gligorijević, Nevenka, Radulović, Siniša, Soković, Marina, "Chemical composition, antimicrobial, and cytotoxic properties of five Lamiaceae essential oils" in Industrial Crops & Products, 61 (2014):225-232,
https://doi.org/10.1016/j.indcrop.2014.07.011 . .
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99

Germline mutation analysis of BRCA1 and BRCA2 genes in Yugoslav breast/ovarian cancer families

Papp, J; Raicević, L; Milašin, Jelena; Dimitrijević, B.; Radulović, Siniša; Olah, E

(1999)

TY  - JOUR
AU  - Papp, J
AU  - Raicević, L
AU  - Milašin, Jelena
AU  - Dimitrijević, B.
AU  - Radulović, Siniša
AU  - Olah, E
PY  - 1999
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1103
AB  - The frequency of germline BRCA1 and BRCA2 mutations was tested in Yugoslav breast and breast/ovarian cancer families using combined heteroduplex/single-strand conformation polymorphism analysis for the entire coding region of both genes. Three different recurrent BRCA1 mutations (one 185delAG, one 3447del4 and two 5382insC) were identified in 4 of 12 families (33%), whereas no definite disease-causing alterations of BRCA2 was detected. Genotype analysis revealed a possible common founder effect for each 185delAG and 5382insC. The relatively high frequency of germline BRCA1 mutations determined in this panel of families confirms the important role of BRCA1 in disease predisposition in the Yugoslav population, while the lack of population specific founder and/or unique mutations show the need of further analysis of samples from this yet unexamined region of Europe.
T2  - Oncology reports
T1  - Germline mutation analysis of BRCA1 and BRCA2 genes in Yugoslav breast/ovarian cancer families
VL  - 6
IS  - 6
SP  - 1435
EP  - 1438
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2281
ER  - 
@article{
author = "Papp, J and Raicević, L and Milašin, Jelena and Dimitrijević, B. and Radulović, Siniša and Olah, E",
year = "1999",
abstract = "The frequency of germline BRCA1 and BRCA2 mutations was tested in Yugoslav breast and breast/ovarian cancer families using combined heteroduplex/single-strand conformation polymorphism analysis for the entire coding region of both genes. Three different recurrent BRCA1 mutations (one 185delAG, one 3447del4 and two 5382insC) were identified in 4 of 12 families (33%), whereas no definite disease-causing alterations of BRCA2 was detected. Genotype analysis revealed a possible common founder effect for each 185delAG and 5382insC. The relatively high frequency of germline BRCA1 mutations determined in this panel of families confirms the important role of BRCA1 in disease predisposition in the Yugoslav population, while the lack of population specific founder and/or unique mutations show the need of further analysis of samples from this yet unexamined region of Europe.",
journal = "Oncology reports",
title = "Germline mutation analysis of BRCA1 and BRCA2 genes in Yugoslav breast/ovarian cancer families",
volume = "6",
number = "6",
pages = "1435-1438",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2281"
}
Papp, J., Raicević, L., Milašin, J., Dimitrijević, B., Radulović, S.,& Olah, E.. (1999). Germline mutation analysis of BRCA1 and BRCA2 genes in Yugoslav breast/ovarian cancer families. in Oncology reports, 6(6), 1435-1438.
https://hdl.handle.net/21.15107/rcub_vinar_2281
Papp J, Raicević L, Milašin J, Dimitrijević B, Radulović S, Olah E. Germline mutation analysis of BRCA1 and BRCA2 genes in Yugoslav breast/ovarian cancer families. in Oncology reports. 1999;6(6):1435-1438.
https://hdl.handle.net/21.15107/rcub_vinar_2281 .
Papp, J, Raicević, L, Milašin, Jelena, Dimitrijević, B., Radulović, Siniša, Olah, E, "Germline mutation analysis of BRCA1 and BRCA2 genes in Yugoslav breast/ovarian cancer families" in Oncology reports, 6, no. 6 (1999):1435-1438,
https://hdl.handle.net/21.15107/rcub_vinar_2281 .
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