TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Andrić, Miroslav
AU  - Nikolić, Nadja
AU  - Corić, V.
AU  - Krezović, S.
AU  - Čarkić, Jelena
AU  - Knežević, Aleksandra
AU  - Beljić-Ivanović, Katarina
AU  - Pljesa-Ercegovac, M.
AU  - Miletić, Maja
AU  - Soldatović, Ivan
AU  - Radosavljević, T.
AU  - Jovanović, T.
AU  - Simić, T.
AU  - Ivanović, Vladimir
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2293
AB  - AimTo investigate whether apical periodontitis lesions infected by Epstein-Barr virus (EBV) exhibit higher levels of oxidative stress biomarkers [8-hydroxydeoxyguanosine (8-OHdG) and oxidized glutathione (GSSG)] and bone resorption regulators [receptor activator of nuclear factor (NF-B) ligand (RANKL) and osteoprotegerin (OPG)] compared to EBV-negative periapical lesions and healthy pulp tissues. MethodologyThe experimental group consisted of 30 EBV-positive and 30 EBV-negative periapical lesions collected in conjunction with apicoectomy. The pulp tissues of 20 impacted third molars were used as healthy controls. The qualitative and quantitative analysis of EBV was performed by nested and real-time polymerase chain reaction (PCR), respectively. The levels of RANKL and OPG were analysed by reverse transcriptase real-time PCR. The levels of 8-OHdG and GSSG were determined by enzyme-linked immunosorbent assay (ELISA). Mann-Whitney U-test and Spearman's correlation were used for statistical analysis. ResultsThe levels of RANKL, OPG, 8-OHdG and GSSG were significantly higher in apical periodontitis lesions compared to healthy pulp controls (P=0.001, P lt 0.001, P lt 0.001 and P lt 0.05, respectively). RANKL and OPG mRNA expression was significantly higher in EBV-positive compared to EBV-negative periapical lesions (P lt 0.05). There was no significant correlation between EBV copy numbers and levels of RANKL, OPG, 8OH-dG and GSSG in apical periodontitis. ConclusionLevels of bone resorption regulators and oxidative stress biomarkers were increased in apical periodontitis compared to healthy pulp tissues. EBV-positive periapical lesions exhibited higher levels of RANKL and OPG compared to EBV-negative periapical lesions. EBV may contribute to progression of apical periodontitis via enhanced production of bone resorption regulators.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by Epstein-Barr virus
VL  - 51
IS  - 6
SP  - 593
EP  - 604
DO  - 10.1111/iej.12886
ER  - 

@article{
author = "Jakovljević, Aleksandar and Andrić, Miroslav and Nikolić, Nadja and Corić, V. and Krezović, S. and Čarkić, Jelena and Knežević, Aleksandra and Beljić-Ivanović, Katarina and Pljesa-Ercegovac, M. and Miletić, Maja and Soldatović, Ivan and Radosavljević, T. and Jovanović, T. and Simić, T. and Ivanović, Vladimir and Milašin, Jelena",
year = "2018",
abstract = "AimTo investigate whether apical periodontitis lesions infected by Epstein-Barr virus (EBV) exhibit higher levels of oxidative stress biomarkers [8-hydroxydeoxyguanosine (8-OHdG) and oxidized glutathione (GSSG)] and bone resorption regulators [receptor activator of nuclear factor (NF-B) ligand (RANKL) and osteoprotegerin (OPG)] compared to EBV-negative periapical lesions and healthy pulp tissues. MethodologyThe experimental group consisted of 30 EBV-positive and 30 EBV-negative periapical lesions collected in conjunction with apicoectomy. The pulp tissues of 20 impacted third molars were used as healthy controls. The qualitative and quantitative analysis of EBV was performed by nested and real-time polymerase chain reaction (PCR), respectively. The levels of RANKL and OPG were analysed by reverse transcriptase real-time PCR. The levels of 8-OHdG and GSSG were determined by enzyme-linked immunosorbent assay (ELISA). Mann-Whitney U-test and Spearman's correlation were used for statistical analysis. ResultsThe levels of RANKL, OPG, 8-OHdG and GSSG were significantly higher in apical periodontitis lesions compared to healthy pulp controls (P=0.001, P lt 0.001, P lt 0.001 and P lt 0.05, respectively). RANKL and OPG mRNA expression was significantly higher in EBV-positive compared to EBV-negative periapical lesions (P lt 0.05). There was no significant correlation between EBV copy numbers and levels of RANKL, OPG, 8OH-dG and GSSG in apical periodontitis. ConclusionLevels of bone resorption regulators and oxidative stress biomarkers were increased in apical periodontitis compared to healthy pulp tissues. EBV-positive periapical lesions exhibited higher levels of RANKL and OPG compared to EBV-negative periapical lesions. EBV may contribute to progression of apical periodontitis via enhanced production of bone resorption regulators.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by Epstein-Barr virus",
volume = "51",
number = "6",
pages = "593-604",
doi = "10.1111/iej.12886"
}

Jakovljević, A., Andrić, M., Nikolić, N., Corić, V., Krezović, S., Čarkić, J., Knežević, A., Beljić-Ivanović, K., Pljesa-Ercegovac, M., Miletić, M., Soldatović, I., Radosavljević, T., Jovanović, T., Simić, T., Ivanović, V.,& Milašin, J.. (2018). Levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by Epstein-Barr virus. in International Endodontic Journal
Wiley, Hoboken., 51(6), 593-604.
https://doi.org/10.1111/iej.12886

Jakovljević A, Andrić M, Nikolić N, Corić V, Krezović S, Čarkić J, Knežević A, Beljić-Ivanović K, Pljesa-Ercegovac M, Miletić M, Soldatović I, Radosavljević T, Jovanović T, Simić T, Ivanović V, Milašin J. Levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by Epstein-Barr virus. in International Endodontic Journal. 2018;51(6):593-604.
doi:10.1111/iej.12886 .

Jakovljević, Aleksandar, Andrić, Miroslav, Nikolić, Nadja, Corić, V., Krezović, S., Čarkić, Jelena, Knežević, Aleksandra, Beljić-Ivanović, Katarina, Pljesa-Ercegovac, M., Miletić, Maja, Soldatović, Ivan, Radosavljević, T., Jovanović, T., Simić, T., Ivanović, Vladimir, Milašin, Jelena, "Levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by Epstein-Barr virus" in International Endodontic Journal, 51, no. 6 (2018):593-604,
https://doi.org/10.1111/iej.12886 . .