TY  - JOUR
AU  - Simonović, Jelena
AU  - Toljić, Boško
AU  - Lazarević, Miloš
AU  - Milošević-Marković, Maja
AU  - Perić, Mina
AU  - Vujin, Jasna
AU  - Panajotović, Radmila
AU  - Milašin, Jelena
PY  - 2022
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/3282
AB  - Background: Dental stem cells, which originate from the neural crest, due to their easy
accessibility might be good candidates in neuro-regenerative procedures, along with graphene-based
nanomaterials shown to promote neurogenesis in vitro. We aimed to explore the potential of liquid-
phase exfoliated graphene (LPEG) film to stimulate the neuro-differentiation of stem cells from apical
papilla (SCAP). Methods: The experimental procedure was structured as follows: (1) fabrication
of graphene film; (2) isolation, cultivation and SCAP stemness characterization by flowcytometry,
multilineage differentiation (osteo, chondro and adipo) and quantitative PCR (qPCR); (3) SCAP
neuro-induction by cultivation on polyethylene terephthalate (PET) coated with graphene film;
(4) evaluation of neural differentiation by means of several microscopy techniques (light, confocal,
atomic force and scanning electron microscopy), followed by neural marker gene expression analysis
using qPCR. Results: SCAP demonstrated exceptional stemness, as judged by mesenchymal markers’
expression (CD73, CD90 and CD105), and by multilineage differentiation capacity (osteo, chondro and
adipo-differentiation). Neuro-induction of SCAP grown on PET coated with graphene film resulted
in neuron-like cellular phenotype observed under different microscopes. This was corroborated
by the high gene expression of all examined key neuronal markers (Ngn2, NF-M, Nestin, MAP2,
MASH1). Conclusions: The ability of SCAPs to differentiate toward neural lineages was markedly
enhanced by graphene film.
PB  - MDPI
T2  - Nanomaterials
T1  - The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla
VL  - 12
IS  - 18
SP  - 3116
DO  - 10.3390/nano12183116
ER  - 

@article{
author = "Simonović, Jelena and Toljić, Boško and Lazarević, Miloš and Milošević-Marković, Maja and Perić, Mina and Vujin, Jasna and Panajotović, Radmila and Milašin, Jelena",
year = "2022",
abstract = "Background: Dental stem cells, which originate from the neural crest, due to their easy
accessibility might be good candidates in neuro-regenerative procedures, along with graphene-based
nanomaterials shown to promote neurogenesis in vitro. We aimed to explore the potential of liquid-
phase exfoliated graphene (LPEG) film to stimulate the neuro-differentiation of stem cells from apical
papilla (SCAP). Methods: The experimental procedure was structured as follows: (1) fabrication
of graphene film; (2) isolation, cultivation and SCAP stemness characterization by flowcytometry,
multilineage differentiation (osteo, chondro and adipo) and quantitative PCR (qPCR); (3) SCAP
neuro-induction by cultivation on polyethylene terephthalate (PET) coated with graphene film;
(4) evaluation of neural differentiation by means of several microscopy techniques (light, confocal,
atomic force and scanning electron microscopy), followed by neural marker gene expression analysis
using qPCR. Results: SCAP demonstrated exceptional stemness, as judged by mesenchymal markers’
expression (CD73, CD90 and CD105), and by multilineage differentiation capacity (osteo, chondro and
adipo-differentiation). Neuro-induction of SCAP grown on PET coated with graphene film resulted
in neuron-like cellular phenotype observed under different microscopes. This was corroborated
by the high gene expression of all examined key neuronal markers (Ngn2, NF-M, Nestin, MAP2,
MASH1). Conclusions: The ability of SCAPs to differentiate toward neural lineages was markedly
enhanced by graphene film.",
publisher = "MDPI",
journal = "Nanomaterials",
title = "The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla",
volume = "12",
number = "18",
pages = "3116",
doi = "10.3390/nano12183116"
}

Simonović, J., Toljić, B., Lazarević, M., Milošević-Marković, M., Perić, M., Vujin, J., Panajotović, R.,& Milašin, J.. (2022). The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla. in Nanomaterials
MDPI., 12(18), 3116.
https://doi.org/10.3390/nano12183116

Simonović J, Toljić B, Lazarević M, Milošević-Marković M, Perić M, Vujin J, Panajotović R, Milašin J. The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla. in Nanomaterials. 2022;12(18):3116.
doi:10.3390/nano12183116 .

Simonović, Jelena, Toljić, Boško, Lazarević, Miloš, Milošević-Marković, Maja, Perić, Mina, Vujin, Jasna, Panajotović, Radmila, Milašin, Jelena, "The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla" in Nanomaterials, 12, no. 18 (2022):3116,
https://doi.org/10.3390/nano12183116 . .

TY  - JOUR
AU  - Simonović, Jelena
AU  - Toljić, Boško
AU  - Rasković, Bozidar
AU  - Jovanović, Vladimir
AU  - Lazarević, Miloš
AU  - Milošević, Maja
AU  - Nikolić, Nadja
AU  - Panajotović, Radmila
AU  - Milašin, Jelena
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2397
AB  - Aim To characterize stem cells originating from different dental tissues (apical papilla [SCAP], dental follicle [DFSC], and pulp [DPSC]) and test the capacity of Raman microspectroscopy to distinguish between the three dental stem cell types. Methods SCAR DFSC, and DPSC cultures were generated from three immature wisdom teeth originating from three patients. Cell stemness was confirmed by inducing neuro-, osteo-, chondro-, and adipo-differentiaton and by mesenchymal marker expression analysis by flow-cytometry and real-time polymerase chain reaction. Cellular components were then evaluated by Raman microspectroscopy. Results We found differences between SCAP, DFSC, and DPSC Raman spectra. The ratio between proteins and nucleic acids (748/770), a parameter for discriminating more differentiated from less differentiated cells, showed significant differences between the three cell types. All cells also displayed a fingerprint region in the 600-700 cm(-1) range, and characteristic lipid peaks at positions 1440 cm(-1) and 1650 cm(-1). Conclusion Although different dental stem cells exhibited similar Raman spectra, the method enabled us to make subtle distinction between them.
PB  - Medicinska Naklada, Zagreb
T2  - Croatian Medical Journal
T1  - Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells
VL  - 60
IS  - 2
SP  - 78
EP  - 86
DO  - 10.3325/CroatMedJ_60_0078
ER  - 

@article{
author = "Simonović, Jelena and Toljić, Boško and Rasković, Bozidar and Jovanović, Vladimir and Lazarević, Miloš and Milošević, Maja and Nikolić, Nadja and Panajotović, Radmila and Milašin, Jelena",
year = "2019",
abstract = "Aim To characterize stem cells originating from different dental tissues (apical papilla [SCAP], dental follicle [DFSC], and pulp [DPSC]) and test the capacity of Raman microspectroscopy to distinguish between the three dental stem cell types. Methods SCAR DFSC, and DPSC cultures were generated from three immature wisdom teeth originating from three patients. Cell stemness was confirmed by inducing neuro-, osteo-, chondro-, and adipo-differentiaton and by mesenchymal marker expression analysis by flow-cytometry and real-time polymerase chain reaction. Cellular components were then evaluated by Raman microspectroscopy. Results We found differences between SCAP, DFSC, and DPSC Raman spectra. The ratio between proteins and nucleic acids (748/770), a parameter for discriminating more differentiated from less differentiated cells, showed significant differences between the three cell types. All cells also displayed a fingerprint region in the 600-700 cm(-1) range, and characteristic lipid peaks at positions 1440 cm(-1) and 1650 cm(-1). Conclusion Although different dental stem cells exhibited similar Raman spectra, the method enabled us to make subtle distinction between them.",
publisher = "Medicinska Naklada, Zagreb",
journal = "Croatian Medical Journal",
title = "Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells",
volume = "60",
number = "2",
pages = "78-86",
doi = "10.3325/CroatMedJ_60_0078"
}

Simonović, J., Toljić, B., Rasković, B., Jovanović, V., Lazarević, M., Milošević, M., Nikolić, N., Panajotović, R.,& Milašin, J.. (2019). Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells. in Croatian Medical Journal
Medicinska Naklada, Zagreb., 60(2), 78-86.
https://doi.org/10.3325/CroatMedJ_60_0078

Simonović J, Toljić B, Rasković B, Jovanović V, Lazarević M, Milošević M, Nikolić N, Panajotović R, Milašin J. Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells. in Croatian Medical Journal. 2019;60(2):78-86.
doi:10.3325/CroatMedJ_60_0078 .

Simonović, Jelena, Toljić, Boško, Rasković, Bozidar, Jovanović, Vladimir, Lazarević, Miloš, Milošević, Maja, Nikolić, Nadja, Panajotović, Radmila, Milašin, Jelena, "Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells" in Croatian Medical Journal, 60, no. 2 (2019):78-86,
https://doi.org/10.3325/CroatMedJ_60_0078 . .

TY  - JOUR
AU  - Lazarević, Miloš
AU  - Milošević, Maja
AU  - Trišić, Dijana
AU  - Toljić, Boško
AU  - Simonović, Jelena
AU  - Nikolić, Nadja
AU  - Miković, Nikola
AU  - Jelovac, Drago
AU  - Petrović, Milan
AU  - Vukadinović, Miroslav
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2271
AB  - Purpose: Recent evidence suggests that small subpopulations of stem-like cells are accountable for tumour initiation, progression and metastasis. Until now, studies were focused exclusively on the characterization of these cell populations within the tumour itself, while tumour margins were neglected, although it is known that the histological and molecular status of tumour margins may play a significant role in the course of the disease. Therefore, the aims of the study were to isolate cells from oral squamous cell carcinomas and their respective margins, to characterize these cells using specific markers, to assess their self-renewal potential and determine their chemoresistance. Methods: Cell cultures were obtained from 12 tissue specimens (6 tumours and 6 margins). Total RNA was extracted and gene expression analysis was done by real-time PCR (RTPCR). Flow cytometry, immunocytometry, sphere formation and MTT assays were also applied. Results: With minor differences, cells originating from both tumours and tumour margins showed the presence of stem cell markers CD133, Nanog, Sox2, CD44, and Oct4, had the capacity to form spheroids and showed chemoresistance. Conclusions: Subpopulations of margin cells appeared to have sternness properties which might raise the question of re-evaluation of optimal surgical management.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma
VL  - 23
IS  - 6
SP  - 1686
EP  - 1692
UR  - https://hdl.handle.net/21.15107/rcub_smile_2271
ER  - 

@article{
author = "Lazarević, Miloš and Milošević, Maja and Trišić, Dijana and Toljić, Boško and Simonović, Jelena and Nikolić, Nadja and Miković, Nikola and Jelovac, Drago and Petrović, Milan and Vukadinović, Miroslav and Milašin, Jelena",
year = "2018",
abstract = "Purpose: Recent evidence suggests that small subpopulations of stem-like cells are accountable for tumour initiation, progression and metastasis. Until now, studies were focused exclusively on the characterization of these cell populations within the tumour itself, while tumour margins were neglected, although it is known that the histological and molecular status of tumour margins may play a significant role in the course of the disease. Therefore, the aims of the study were to isolate cells from oral squamous cell carcinomas and their respective margins, to characterize these cells using specific markers, to assess their self-renewal potential and determine their chemoresistance. Methods: Cell cultures were obtained from 12 tissue specimens (6 tumours and 6 margins). Total RNA was extracted and gene expression analysis was done by real-time PCR (RTPCR). Flow cytometry, immunocytometry, sphere formation and MTT assays were also applied. Results: With minor differences, cells originating from both tumours and tumour margins showed the presence of stem cell markers CD133, Nanog, Sox2, CD44, and Oct4, had the capacity to form spheroids and showed chemoresistance. Conclusions: Subpopulations of margin cells appeared to have sternness properties which might raise the question of re-evaluation of optimal surgical management.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma",
volume = "23",
number = "6",
pages = "1686-1692",
url = "https://hdl.handle.net/21.15107/rcub_smile_2271"
}

Lazarević, M., Milošević, M., Trišić, D., Toljić, B., Simonović, J., Nikolić, N., Miković, N., Jelovac, D., Petrović, M., Vukadinović, M.,& Milašin, J.. (2018). Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 23(6), 1686-1692.
https://hdl.handle.net/21.15107/rcub_smile_2271

Lazarević M, Milošević M, Trišić D, Toljić B, Simonović J, Nikolić N, Miković N, Jelovac D, Petrović M, Vukadinović M, Milašin J. Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma. in Journal of BUON. 2018;23(6):1686-1692.
https://hdl.handle.net/21.15107/rcub_smile_2271 .

Lazarević, Miloš, Milošević, Maja, Trišić, Dijana, Toljić, Boško, Simonović, Jelena, Nikolić, Nadja, Miković, Nikola, Jelovac, Drago, Petrović, Milan, Vukadinović, Miroslav, Milašin, Jelena, "Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma" in Journal of BUON, 23, no. 6 (2018):1686-1692,
https://hdl.handle.net/21.15107/rcub_smile_2271 .

TY  - JOUR
AU  - Simonović, Jelena
AU  - Toljić, Boško
AU  - Nikolić, Nadja
AU  - Perić, Mina
AU  - Vujin, Jasna
AU  - Panajotović, Radmila
AU  - Gajić, Radoš
AU  - Bekyarova, Elena
AU  - Cataldi, Amelia
AU  - Parpura, Vladimir
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2327
AB  - Stem cell-based therapies are considered a promising treatment modality for many medical conditions. Several types of stem cells with variable differentiation potentials have been isolated from dental tissues, among them stem cells from apical papilla (SCAP). In parallel, new classes of biocompatible nanomaterials have also been developed, including graphene and carbon nanotube-based materials. The aim of the study was to assess whether graphene dispersion (GD) and water-soluble single walled carbon nanotubes (ws-SWCNT), may enhance SCAPs capacity to undergo neural differentiation. SCAPs cultivated in neuroinductive medium supplemented with GD and ws-SWCNT, separately and in combination, were subjected to neural marker analysis by real-time polymerase chain reaction (neurofilament medium [NF-M], neurogenin-2 [ngn-2], III-tubulin, microtubule-associated protein 2) and immunocytochemistry (NeuN and III-tubulin). GD, ws-SWCNT, and their combination, had neuro-stimulatory effects on SCAPs, as judged by the production of neural markers. Compared to cells grown in nanomaterial free medium, cells with GD showed higher production of B3T, cells with ws-SWCNT had higher production of ngn-2 and NF-M, while the combination of nanomaterials gave similar levels of both B3T and NF-M as the neuroinductive medium aloal ne, but with the finest neuron-like morphology. In conclusion, GD and ws-SWCNT seem to enhance neurdifferentiation of SCAP.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes
VL  - 106
IS  - 10
SP  - 2653
EP  - 2661
DO  - 10.1002/jbm.a.36461
ER  - 

@article{
author = "Simonović, Jelena and Toljić, Boško and Nikolić, Nadja and Perić, Mina and Vujin, Jasna and Panajotović, Radmila and Gajić, Radoš and Bekyarova, Elena and Cataldi, Amelia and Parpura, Vladimir and Milašin, Jelena",
year = "2018",
abstract = "Stem cell-based therapies are considered a promising treatment modality for many medical conditions. Several types of stem cells with variable differentiation potentials have been isolated from dental tissues, among them stem cells from apical papilla (SCAP). In parallel, new classes of biocompatible nanomaterials have also been developed, including graphene and carbon nanotube-based materials. The aim of the study was to assess whether graphene dispersion (GD) and water-soluble single walled carbon nanotubes (ws-SWCNT), may enhance SCAPs capacity to undergo neural differentiation. SCAPs cultivated in neuroinductive medium supplemented with GD and ws-SWCNT, separately and in combination, were subjected to neural marker analysis by real-time polymerase chain reaction (neurofilament medium [NF-M], neurogenin-2 [ngn-2], III-tubulin, microtubule-associated protein 2) and immunocytochemistry (NeuN and III-tubulin). GD, ws-SWCNT, and their combination, had neuro-stimulatory effects on SCAPs, as judged by the production of neural markers. Compared to cells grown in nanomaterial free medium, cells with GD showed higher production of B3T, cells with ws-SWCNT had higher production of ngn-2 and NF-M, while the combination of nanomaterials gave similar levels of both B3T and NF-M as the neuroinductive medium aloal ne, but with the finest neuron-like morphology. In conclusion, GD and ws-SWCNT seem to enhance neurdifferentiation of SCAP.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes",
volume = "106",
number = "10",
pages = "2653-2661",
doi = "10.1002/jbm.a.36461"
}

Simonović, J., Toljić, B., Nikolić, N., Perić, M., Vujin, J., Panajotović, R., Gajić, R., Bekyarova, E., Cataldi, A., Parpura, V.,& Milašin, J.. (2018). Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 106(10), 2653-2661.
https://doi.org/10.1002/jbm.a.36461

Simonović J, Toljić B, Nikolić N, Perić M, Vujin J, Panajotović R, Gajić R, Bekyarova E, Cataldi A, Parpura V, Milašin J. Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes. in Journal of Biomedical Materials Research Part A. 2018;106(10):2653-2661.
doi:10.1002/jbm.a.36461 .

Simonović, Jelena, Toljić, Boško, Nikolić, Nadja, Perić, Mina, Vujin, Jasna, Panajotović, Radmila, Gajić, Radoš, Bekyarova, Elena, Cataldi, Amelia, Parpura, Vladimir, Milašin, Jelena, "Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes" in Journal of Biomedical Materials Research Part A, 106, no. 10 (2018):2653-2661,
https://doi.org/10.1002/jbm.a.36461 . .

TY  - JOUR
AU  - Milošević, Maja
AU  - Lazarević, Miloš
AU  - Toljić, Boško
AU  - Simonović, Jelena
AU  - Trišić, Dijana
AU  - Nikolić, Nadja
AU  - Petrović, Milan
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2279
AB  - BackgroundUnderstanding the pathogenesis of basal cell carcinoma (BCC) and identifying the cells responsible for propagation and recurrence are crucial for the development of new treatment strategies. The aim of this study was to characterize the cells isolated from BCC and its margin. MethodsPrimary cultures were established from 10 BCCs, their respective close resection margins (3mm) and 10 control tissues. Stem cell markers analysis was carried out by real-time PCR and/or flow cytometry. Spheroid formation and MTT assays were also performed. ResultsReal-time PCR showed a higher expression of embryonic (Oct4, Sox2 and Nanog) and mesenchymal (CD44 and CD73) stem cell markers in tumors compared to margins and controls (P lt 0.05). Bmi-1 and GPR49 were also upregulated in tumors in comparison with margins. Both tumor and margin cells, but not normal, had the capacity to form spheroids. During passages, the number of spheres increased, while the diameter decreased. Tumor cells showed higher chemo-resistance compared to margin and control cells. ConclusionsBasal cell carcinomas expressed stem cell markers, pointing to the existence of a cancer cell side population with stemness characteristics. Margin also appeared to harbour a small number of cancer-initiating cells.
PB  - Wiley, Hoboken
T2  - Experimental Dermatology
T1  - Characterization of stem-like cancer cells in basal cell carcinoma and its surgical margins
VL  - 27
IS  - 10
SP  - 1160
EP  - 1165
DO  - 10.1111/exd.13755
ER  - 

@article{
author = "Milošević, Maja and Lazarević, Miloš and Toljić, Boško and Simonović, Jelena and Trišić, Dijana and Nikolić, Nadja and Petrović, Milan and Milašin, Jelena",
year = "2018",
abstract = "BackgroundUnderstanding the pathogenesis of basal cell carcinoma (BCC) and identifying the cells responsible for propagation and recurrence are crucial for the development of new treatment strategies. The aim of this study was to characterize the cells isolated from BCC and its margin. MethodsPrimary cultures were established from 10 BCCs, their respective close resection margins (3mm) and 10 control tissues. Stem cell markers analysis was carried out by real-time PCR and/or flow cytometry. Spheroid formation and MTT assays were also performed. ResultsReal-time PCR showed a higher expression of embryonic (Oct4, Sox2 and Nanog) and mesenchymal (CD44 and CD73) stem cell markers in tumors compared to margins and controls (P lt 0.05). Bmi-1 and GPR49 were also upregulated in tumors in comparison with margins. Both tumor and margin cells, but not normal, had the capacity to form spheroids. During passages, the number of spheres increased, while the diameter decreased. Tumor cells showed higher chemo-resistance compared to margin and control cells. ConclusionsBasal cell carcinomas expressed stem cell markers, pointing to the existence of a cancer cell side population with stemness characteristics. Margin also appeared to harbour a small number of cancer-initiating cells.",
publisher = "Wiley, Hoboken",
journal = "Experimental Dermatology",
title = "Characterization of stem-like cancer cells in basal cell carcinoma and its surgical margins",
volume = "27",
number = "10",
pages = "1160-1165",
doi = "10.1111/exd.13755"
}

Milošević, M., Lazarević, M., Toljić, B., Simonović, J., Trišić, D., Nikolić, N., Petrović, M.,& Milašin, J.. (2018). Characterization of stem-like cancer cells in basal cell carcinoma and its surgical margins. in Experimental Dermatology
Wiley, Hoboken., 27(10), 1160-1165.
https://doi.org/10.1111/exd.13755

Milošević M, Lazarević M, Toljić B, Simonović J, Trišić D, Nikolić N, Petrović M, Milašin J. Characterization of stem-like cancer cells in basal cell carcinoma and its surgical margins. in Experimental Dermatology. 2018;27(10):1160-1165.
doi:10.1111/exd.13755 .

Milošević, Maja, Lazarević, Miloš, Toljić, Boško, Simonović, Jelena, Trišić, Dijana, Nikolić, Nadja, Petrović, Milan, Milašin, Jelena, "Characterization of stem-like cancer cells in basal cell carcinoma and its surgical margins" in Experimental Dermatology, 27, no. 10 (2018):1160-1165,
https://doi.org/10.1111/exd.13755 . .

TY  - JOUR
AU  - Nikolić, Nadja
AU  - Aničić, Boban
AU  - Čarkić, Jelena
AU  - Simonović, Jelena
AU  - Toljić, Boško
AU  - Tanić, Nasta
AU  - Tepavčević, Zvezdana
AU  - Vukadinović, Miroslav
AU  - Konstantinović, Vitomir
AU  - Milašin, Jelena
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2019
AB  - Objectives: to investigate p16(INK4a) and p14(ARF) tumor suppressor gene methylation status, determine telomere length and assess the importance of these epigenetic and genetic parameters in the development of pleomorphic adenoma and carcinoma ex pleomorphic adenoma of the parotid salivary glands. Materials and Methods: Genomic DNA from paraffin-embedded samples of 50 pleomorphic adenomas and 10 carcinomas ex pleomorphic adenoma was subjected to methylation specific polymerase chain reaction for hypermethylation analyses and real time polymerase chain reaction for the relative telomere length calculations. Results: Promoter hypermethylation of the two genes was a very frequent event in both neoplasms between 60% and 90% of samples were hypermethylated - but without significant difference between the groups. The mean relative telomere length in the pleomorphic adenoma group was significantly increased in comparison to the control group (P = 0.00), and significantly decreased in comparison to the carcinoma group (P = 0.05). Telomeres were also longer in myxoid and cellular histological subtypes of adenomas than in the classic type (P = 0.044 and P = 0.018, respectively). Longer telomeres were more frequent in tumors with hypermethylated p14(ARF) alleles (P = 0.013). Conclusion: Promoter hypermethylations seems to be an important mechanism of p16(INK4a) and Pl4(ARF) inactivation in parotid gland tumors. Telomeric lengthening appears to be involved in the pathogenesis of both benign and malignant tumors of the parotid glands.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Archives of Oral Biology
T1  - High frequency of p16 and p14 promoter hypermethylation and marked telomere instability in salivary gland tumors
VL  - 60
IS  - 11
SP  - 1662
EP  - 1666
DO  - 10.1016/j.archoralbio.2015.08.011
ER  - 

@article{
author = "Nikolić, Nadja and Aničić, Boban and Čarkić, Jelena and Simonović, Jelena and Toljić, Boško and Tanić, Nasta and Tepavčević, Zvezdana and Vukadinović, Miroslav and Konstantinović, Vitomir and Milašin, Jelena",
year = "2015",
abstract = "Objectives: to investigate p16(INK4a) and p14(ARF) tumor suppressor gene methylation status, determine telomere length and assess the importance of these epigenetic and genetic parameters in the development of pleomorphic adenoma and carcinoma ex pleomorphic adenoma of the parotid salivary glands. Materials and Methods: Genomic DNA from paraffin-embedded samples of 50 pleomorphic adenomas and 10 carcinomas ex pleomorphic adenoma was subjected to methylation specific polymerase chain reaction for hypermethylation analyses and real time polymerase chain reaction for the relative telomere length calculations. Results: Promoter hypermethylation of the two genes was a very frequent event in both neoplasms between 60% and 90% of samples were hypermethylated - but without significant difference between the groups. The mean relative telomere length in the pleomorphic adenoma group was significantly increased in comparison to the control group (P = 0.00), and significantly decreased in comparison to the carcinoma group (P = 0.05). Telomeres were also longer in myxoid and cellular histological subtypes of adenomas than in the classic type (P = 0.044 and P = 0.018, respectively). Longer telomeres were more frequent in tumors with hypermethylated p14(ARF) alleles (P = 0.013). Conclusion: Promoter hypermethylations seems to be an important mechanism of p16(INK4a) and Pl4(ARF) inactivation in parotid gland tumors. Telomeric lengthening appears to be involved in the pathogenesis of both benign and malignant tumors of the parotid glands.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Archives of Oral Biology",
title = "High frequency of p16 and p14 promoter hypermethylation and marked telomere instability in salivary gland tumors",
volume = "60",
number = "11",
pages = "1662-1666",
doi = "10.1016/j.archoralbio.2015.08.011"
}

Nikolić, N., Aničić, B., Čarkić, J., Simonović, J., Toljić, B., Tanić, N., Tepavčević, Z., Vukadinović, M., Konstantinović, V.,& Milašin, J.. (2015). High frequency of p16 and p14 promoter hypermethylation and marked telomere instability in salivary gland tumors. in Archives of Oral Biology
Pergamon-Elsevier Science Ltd, Oxford., 60(11), 1662-1666.
https://doi.org/10.1016/j.archoralbio.2015.08.011

Nikolić N, Aničić B, Čarkić J, Simonović J, Toljić B, Tanić N, Tepavčević Z, Vukadinović M, Konstantinović V, Milašin J. High frequency of p16 and p14 promoter hypermethylation and marked telomere instability in salivary gland tumors. in Archives of Oral Biology. 2015;60(11):1662-1666.
doi:10.1016/j.archoralbio.2015.08.011 .

Nikolić, Nadja, Aničić, Boban, Čarkić, Jelena, Simonović, Jelena, Toljić, Boško, Tanić, Nasta, Tepavčević, Zvezdana, Vukadinović, Miroslav, Konstantinović, Vitomir, Milašin, Jelena, "High frequency of p16 and p14 promoter hypermethylation and marked telomere instability in salivary gland tumors" in Archives of Oral Biology, 60, no. 11 (2015):1662-1666,
https://doi.org/10.1016/j.archoralbio.2015.08.011 . .