TY  - JOUR
AU  - Andrić, Miroslav
AU  - Jaćimović, Jelena
AU  - Jakovljević, Aleksandar
AU  - Nikolić, Nadja
AU  - Milašin, Jelena
PY  - 2021
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2578
AB  - Objectives
The aim of this systematic review was to critically analyze available data on gene polymorphisms in odontogenic keratocysts (OKC) and ameloblastomas, including their possible relationship with clinical and histological features of these lesions.

Materials and Methods
A comprehensive search of Web of Science Scopus, PubMed, Cochrane Central Register of Controlled Trials and EMBASE was conducted using relevant key terms and supplemented by a gray literature search. Quality assessment of included studies was performed using criteria from the Strengthening the Reporting of Genetic Association (STREGA) statement.

Results
Ten studies were included in the final review. Survivin ‐31G/C, interleukin IL‐1α ‐889 C/T, p53 codon 72 G/C, tumor necrosis factor TNF‐α (−308G>A) and its receptor TNF‐R1 (36A>G), glioma‐associated oncogene homolog 1 rs2228224 and matrix metalloproteinase 2 rs243865 gene polymorphisms were reported to be associated with OKC. For ameloblastomas, p53 codon 72 G/C, X‐ray repair cross‐complementing protein 1—codons 194 and 399 and matrix metalloproteinase 9 rs3918242 gene polymorphisms were identified as risk factors. It was not possible to establish a relationship between specific polymorphisms and clinical and histological features of investigated lesions.

Conclusions
Several gene polymorphisms might be considered as a risk factor for the development of these lesions. Future studies should investigate whether these polymorphisms might be used to identify patients with increased risk of recurrence or aggressive disease.
PB  - Wiley
T2  - Oral Diseases
T1  - Gene polymorphisms in odontogenic keratocysts and ameloblastomas: A systematic review
DO  - 10.1111/odi.13865
ER  - 

@article{
author = "Andrić, Miroslav and Jaćimović, Jelena and Jakovljević, Aleksandar and Nikolić, Nadja and Milašin, Jelena",
year = "2021",
abstract = "Objectives
The aim of this systematic review was to critically analyze available data on gene polymorphisms in odontogenic keratocysts (OKC) and ameloblastomas, including their possible relationship with clinical and histological features of these lesions.

Materials and Methods
A comprehensive search of Web of Science Scopus, PubMed, Cochrane Central Register of Controlled Trials and EMBASE was conducted using relevant key terms and supplemented by a gray literature search. Quality assessment of included studies was performed using criteria from the Strengthening the Reporting of Genetic Association (STREGA) statement.

Results
Ten studies were included in the final review. Survivin ‐31G/C, interleukin IL‐1α ‐889 C/T, p53 codon 72 G/C, tumor necrosis factor TNF‐α (−308G>A) and its receptor TNF‐R1 (36A>G), glioma‐associated oncogene homolog 1 rs2228224 and matrix metalloproteinase 2 rs243865 gene polymorphisms were reported to be associated with OKC. For ameloblastomas, p53 codon 72 G/C, X‐ray repair cross‐complementing protein 1—codons 194 and 399 and matrix metalloproteinase 9 rs3918242 gene polymorphisms were identified as risk factors. It was not possible to establish a relationship between specific polymorphisms and clinical and histological features of investigated lesions.

Conclusions
Several gene polymorphisms might be considered as a risk factor for the development of these lesions. Future studies should investigate whether these polymorphisms might be used to identify patients with increased risk of recurrence or aggressive disease.",
publisher = "Wiley",
journal = "Oral Diseases",
title = "Gene polymorphisms in odontogenic keratocysts and ameloblastomas: A systematic review",
doi = "10.1111/odi.13865"
}

Andrić, M., Jaćimović, J., Jakovljević, A., Nikolić, N.,& Milašin, J.. (2021). Gene polymorphisms in odontogenic keratocysts and ameloblastomas: A systematic review. in Oral Diseases
Wiley..
https://doi.org/10.1111/odi.13865

Andrić M, Jaćimović J, Jakovljević A, Nikolić N, Milašin J. Gene polymorphisms in odontogenic keratocysts and ameloblastomas: A systematic review. in Oral Diseases. 2021;.
doi:10.1111/odi.13865 .

Andrić, Miroslav, Jaćimović, Jelena, Jakovljević, Aleksandar, Nikolić, Nadja, Milašin, Jelena, "Gene polymorphisms in odontogenic keratocysts and ameloblastomas: A systematic review" in Oral Diseases (2021),
https://doi.org/10.1111/odi.13865 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Nikolić, Nadja
AU  - Jaćimović, Jelena
AU  - Miletić, Maja
AU  - Andrić, Miroslav
AU  - Milašin, Jelena
AU  - Aminoshariae, Anita
AU  - Azarpazhooh, Amir
PY  - 2021
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2577
AB  - Introduction
This study aimed to perform a more precise estimation of the association between tumor necrosis factor-alpha (TNF-α) – 308 G/A single nucleotide polymorphism (SNP) and the risk of development of AP and its phenotypes based on all available published studies.
Methods
The study was carried out according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and is registered in PROSPERO (CRD42020176190). The literature search was conducted via: Clarivate Analytics’ Scopus, PubMed, Cochrane Central Register of Controlled Trials and China National Knowledge Infrastructure databases, from inception to December 2020 with no language restrictions. Two reviewers were involved independently in study selection, data extraction and appraising the studies that were included. The quality of included studies was evaluated using the Strengthening the Reporting of Genetic Association (STREGA) and the Grading of Recommendations Assessment, Development and Evaluation (GRADE) system. Frequencies of genotypes and alleles of TNF-alpha (G>A 308, rs1800629) gene, with 95% Odds ratio was used.
Results
Four studies met the inclusion criteria with moderate risk of bias. This study revealed no significant association between TNF-α – 308 G/A SNP and AP, and the risk of AP development. Moreover, there was no significant association between genotype or allele frequency distribution and clinical manifestations (acute versus chronic) of AP. The certainty of evidence per GRADE was very low.
Conclusions
Due to very low certainty of evidence, whether there is an association between TNF-α – 308 G/A SNP and AP, warrants further well-designed multi-centric studies to adjudicate a better understanding of the role of genetic factors in the etiopathogenesis of AP.
PB  - Elsevier
T2  - Journal of Endodontics
T1  - TNF-α -308 G/A single nucleotide polymorphism and apical periodontitis: an updated systematic review and meta-analysis
DO  - 10.1016/j.joen.2021.03.007
ER  - 

@article{
author = "Jakovljević, Aleksandar and Nikolić, Nadja and Jaćimović, Jelena and Miletić, Maja and Andrić, Miroslav and Milašin, Jelena and Aminoshariae, Anita and Azarpazhooh, Amir",
year = "2021",
abstract = "Introduction
This study aimed to perform a more precise estimation of the association between tumor necrosis factor-alpha (TNF-α) – 308 G/A single nucleotide polymorphism (SNP) and the risk of development of AP and its phenotypes based on all available published studies.
Methods
The study was carried out according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and is registered in PROSPERO (CRD42020176190). The literature search was conducted via: Clarivate Analytics’ Scopus, PubMed, Cochrane Central Register of Controlled Trials and China National Knowledge Infrastructure databases, from inception to December 2020 with no language restrictions. Two reviewers were involved independently in study selection, data extraction and appraising the studies that were included. The quality of included studies was evaluated using the Strengthening the Reporting of Genetic Association (STREGA) and the Grading of Recommendations Assessment, Development and Evaluation (GRADE) system. Frequencies of genotypes and alleles of TNF-alpha (G>A 308, rs1800629) gene, with 95% Odds ratio was used.
Results
Four studies met the inclusion criteria with moderate risk of bias. This study revealed no significant association between TNF-α – 308 G/A SNP and AP, and the risk of AP development. Moreover, there was no significant association between genotype or allele frequency distribution and clinical manifestations (acute versus chronic) of AP. The certainty of evidence per GRADE was very low.
Conclusions
Due to very low certainty of evidence, whether there is an association between TNF-α – 308 G/A SNP and AP, warrants further well-designed multi-centric studies to adjudicate a better understanding of the role of genetic factors in the etiopathogenesis of AP.",
publisher = "Elsevier",
journal = "Journal of Endodontics",
title = "TNF-α -308 G/A single nucleotide polymorphism and apical periodontitis: an updated systematic review and meta-analysis",
doi = "10.1016/j.joen.2021.03.007"
}

Jakovljević, A., Nikolić, N., Jaćimović, J., Miletić, M., Andrić, M., Milašin, J., Aminoshariae, A.,& Azarpazhooh, A.. (2021). TNF-α -308 G/A single nucleotide polymorphism and apical periodontitis: an updated systematic review and meta-analysis. in Journal of Endodontics
Elsevier..
https://doi.org/10.1016/j.joen.2021.03.007

Jakovljević A, Nikolić N, Jaćimović J, Miletić M, Andrić M, Milašin J, Aminoshariae A, Azarpazhooh A. TNF-α -308 G/A single nucleotide polymorphism and apical periodontitis: an updated systematic review and meta-analysis. in Journal of Endodontics. 2021;.
doi:10.1016/j.joen.2021.03.007 .

Jakovljević, Aleksandar, Nikolić, Nadja, Jaćimović, Jelena, Miletić, Maja, Andrić, Miroslav, Milašin, Jelena, Aminoshariae, Anita, Azarpazhooh, Amir, "TNF-α -308 G/A single nucleotide polymorphism and apical periodontitis: an updated systematic review and meta-analysis" in Journal of Endodontics (2021),
https://doi.org/10.1016/j.joen.2021.03.007 . .

TY  - JOUR
AU  - Đinić Krasavčević, Ana
AU  - Nikolić, Nadja
AU  - Mijailović, Iva
AU  - Čarkić, Jelena
AU  - Milinković, Iva
AU  - Janković, Saša
AU  - Aleksić, Zoran
AU  - Milašin, Jelena
PY  - 2021
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2568
AB  - Background and objective
Notch signalling cascade has recently been connected to alveolar bone resorption in periodontitis. Hence, the present cross‐sectional study aimed to analyze the expression of Notch signalling pathway (Notch 1, Notch 2, Jagged 1, Hes 1, Hey 1) and periodontitis‐related (tumor necrosis factor alpha‐ TNF‐α, interleukin 17‐IL‐17, receptor activator of nuclear factor‐kappa B ligand—RANKL, osteoprotegerin—OPG) molecules and correlate it with clinical parameters in aggressive (AP) and chronic (CP) periodontitis. Additionally, the aforementioned markers' expression was evaluated in periodontitis patients with different RANKL/OPG ratios.

Material and methods
Eighty patients were enrolled either in AP or CP group. Clinical attachment level (CAL), bleeding on probing (BOP), periodontal probing depth (PPD) and plaque index (PI) were recorded for each patient. Total RNA was extracted from gingival crevicular fluid samples. Relative gene expression of investigated markers was determined by reverse transcriptase‐real‐time polymerase chain reaction.

Results
Significantly higher values of PPD were observed in AP compared to CP (P = .010). Negative correlations between OPG and CAL, and OPG and PI, were found in AP (P = .045, P = .006, respectively), while Hey 1 and PI had a positive correlation (P = .049). In multivariate linear regression analysis, OPG and Notch 2 were predictors of CAL in AP group. TNF‐α and IL‐17 were higher in RANKL predominant than in OPG predominant cases (P = .007, P = .001, respectively). In RANKL predominant lesions Notch 1 and Jagged 1 were down‐regulated in AP compared to CP patients (P = .010, P = .025, respectively).

Conclusion
The present study demonstrated that changes in Notch 2 expression affected CAL in AP cases hence this molecule could be considered as a contributor to alveolar bone loss. In RANKL‐activated settings, the down‐regulation of Notch 1 might participate in more severe bone resorption in AP.
PB  - Wiley
T2  - Journal of Periodontal Research
T1  - Impact of Notch signalling molecules and bone resorption regulators on clinical parameters in periodontitis
VL  - 56
IS  - 1
SP  - 131
EP  - 138
DO  - 10.1111/jre.12801
ER  - 

@article{
author = "Đinić Krasavčević, Ana and Nikolić, Nadja and Mijailović, Iva and Čarkić, Jelena and Milinković, Iva and Janković, Saša and Aleksić, Zoran and Milašin, Jelena",
year = "2021",
abstract = "Background and objective
Notch signalling cascade has recently been connected to alveolar bone resorption in periodontitis. Hence, the present cross‐sectional study aimed to analyze the expression of Notch signalling pathway (Notch 1, Notch 2, Jagged 1, Hes 1, Hey 1) and periodontitis‐related (tumor necrosis factor alpha‐ TNF‐α, interleukin 17‐IL‐17, receptor activator of nuclear factor‐kappa B ligand—RANKL, osteoprotegerin—OPG) molecules and correlate it with clinical parameters in aggressive (AP) and chronic (CP) periodontitis. Additionally, the aforementioned markers' expression was evaluated in periodontitis patients with different RANKL/OPG ratios.

Material and methods
Eighty patients were enrolled either in AP or CP group. Clinical attachment level (CAL), bleeding on probing (BOP), periodontal probing depth (PPD) and plaque index (PI) were recorded for each patient. Total RNA was extracted from gingival crevicular fluid samples. Relative gene expression of investigated markers was determined by reverse transcriptase‐real‐time polymerase chain reaction.

Results
Significantly higher values of PPD were observed in AP compared to CP (P = .010). Negative correlations between OPG and CAL, and OPG and PI, were found in AP (P = .045, P = .006, respectively), while Hey 1 and PI had a positive correlation (P = .049). In multivariate linear regression analysis, OPG and Notch 2 were predictors of CAL in AP group. TNF‐α and IL‐17 were higher in RANKL predominant than in OPG predominant cases (P = .007, P = .001, respectively). In RANKL predominant lesions Notch 1 and Jagged 1 were down‐regulated in AP compared to CP patients (P = .010, P = .025, respectively).

Conclusion
The present study demonstrated that changes in Notch 2 expression affected CAL in AP cases hence this molecule could be considered as a contributor to alveolar bone loss. In RANKL‐activated settings, the down‐regulation of Notch 1 might participate in more severe bone resorption in AP.",
publisher = "Wiley",
journal = "Journal of Periodontal Research",
title = "Impact of Notch signalling molecules and bone resorption regulators on clinical parameters in periodontitis",
volume = "56",
number = "1",
pages = "131-138",
doi = "10.1111/jre.12801"
}

Đinić Krasavčević, A., Nikolić, N., Mijailović, I., Čarkić, J., Milinković, I., Janković, S., Aleksić, Z.,& Milašin, J.. (2021). Impact of Notch signalling molecules and bone resorption regulators on clinical parameters in periodontitis. in Journal of Periodontal Research
Wiley., 56(1), 131-138.
https://doi.org/10.1111/jre.12801

Đinić Krasavčević A, Nikolić N, Mijailović I, Čarkić J, Milinković I, Janković S, Aleksić Z, Milašin J. Impact of Notch signalling molecules and bone resorption regulators on clinical parameters in periodontitis. in Journal of Periodontal Research. 2021;56(1):131-138.
doi:10.1111/jre.12801 .

Đinić Krasavčević, Ana, Nikolić, Nadja, Mijailović, Iva, Čarkić, Jelena, Milinković, Iva, Janković, Saša, Aleksić, Zoran, Milašin, Jelena, "Impact of Notch signalling molecules and bone resorption regulators on clinical parameters in periodontitis" in Journal of Periodontal Research, 56, no. 1 (2021):131-138,
https://doi.org/10.1111/jre.12801 . .

TY  - JOUR
AU  - Dragović, Miroslav
AU  - Pejović, Marko
AU  - Stepić, Jelena
AU  - Čolić, Snježana
AU  - Dožić, Branko
AU  - Dragović, Svetlana
AU  - Lazarević, Miloš
AU  - Nikolić, Nadja
AU  - Milašin, Jelena
AU  - Miličić, Biljana
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2513
AB  - Objectives Sutures are the most frequently used medical device for wound closure. They support tissue during the early phase of healing until it regains enough tensile strength. The aim of this study was to compare four different suture materials in terms of the influence on wound healing, microbial adherence, tissue reaction, and relevant clinical parameters which determine their clinical value. Materials and Methods Total number of 32 patients undergoing surgical extraction of four impacted third molars were involved in the study. Clinical parameters were estimated intraoperatively and during the control check-ups. Soft tissue healing around sutures were evaluated on the 3rd and 7th day postoperatively. Microbial colonization was assessed by means of qPCR. Also, histological analysis was done to assess inflammatory reaction. Results Significantly better soft tissue healing was found around monofilament and synthetic sutures compared to multifilament and natural ones respectively. Soft tissue healing was significantly better around all sutures on the 7th day than on the 3rd day postoperatively. Conclusions Non-resorbable polypropylene suture showed superior clinical characteristics among all sutures. Moreover, the best healing of soft tissue and the least inflammatory reaction was found around this thread. The poorest soft tissue healing was found around non-resorbable silk suture. This suture elicited strongest inflammatory reaction and showed the greatest microbial adherence affinity compared to alternative sutures.
PB  - Springer Heidelberg, Heidelberg
T2  - Clinical Oral Investigations
T1  - Comparison of four different suture materials in respect to oral wound healing, microbial colonization, tissue reaction and clinical features-randomized clinical study
VL  - 24
IS  - 4
SP  - 1527
EP  - 1541
DO  - 10.1007/s00784-019-03034-4
ER  - 

@article{
author = "Dragović, Miroslav and Pejović, Marko and Stepić, Jelena and Čolić, Snježana and Dožić, Branko and Dragović, Svetlana and Lazarević, Miloš and Nikolić, Nadja and Milašin, Jelena and Miličić, Biljana",
year = "2020",
abstract = "Objectives Sutures are the most frequently used medical device for wound closure. They support tissue during the early phase of healing until it regains enough tensile strength. The aim of this study was to compare four different suture materials in terms of the influence on wound healing, microbial adherence, tissue reaction, and relevant clinical parameters which determine their clinical value. Materials and Methods Total number of 32 patients undergoing surgical extraction of four impacted third molars were involved in the study. Clinical parameters were estimated intraoperatively and during the control check-ups. Soft tissue healing around sutures were evaluated on the 3rd and 7th day postoperatively. Microbial colonization was assessed by means of qPCR. Also, histological analysis was done to assess inflammatory reaction. Results Significantly better soft tissue healing was found around monofilament and synthetic sutures compared to multifilament and natural ones respectively. Soft tissue healing was significantly better around all sutures on the 7th day than on the 3rd day postoperatively. Conclusions Non-resorbable polypropylene suture showed superior clinical characteristics among all sutures. Moreover, the best healing of soft tissue and the least inflammatory reaction was found around this thread. The poorest soft tissue healing was found around non-resorbable silk suture. This suture elicited strongest inflammatory reaction and showed the greatest microbial adherence affinity compared to alternative sutures.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Clinical Oral Investigations",
title = "Comparison of four different suture materials in respect to oral wound healing, microbial colonization, tissue reaction and clinical features-randomized clinical study",
volume = "24",
number = "4",
pages = "1527-1541",
doi = "10.1007/s00784-019-03034-4"
}

Dragović, M., Pejović, M., Stepić, J., Čolić, S., Dožić, B., Dragović, S., Lazarević, M., Nikolić, N., Milašin, J.,& Miličić, B.. (2020). Comparison of four different suture materials in respect to oral wound healing, microbial colonization, tissue reaction and clinical features-randomized clinical study. in Clinical Oral Investigations
Springer Heidelberg, Heidelberg., 24(4), 1527-1541.
https://doi.org/10.1007/s00784-019-03034-4

Dragović M, Pejović M, Stepić J, Čolić S, Dožić B, Dragović S, Lazarević M, Nikolić N, Milašin J, Miličić B. Comparison of four different suture materials in respect to oral wound healing, microbial colonization, tissue reaction and clinical features-randomized clinical study. in Clinical Oral Investigations. 2020;24(4):1527-1541.
doi:10.1007/s00784-019-03034-4 .

Dragović, Miroslav, Pejović, Marko, Stepić, Jelena, Čolić, Snježana, Dožić, Branko, Dragović, Svetlana, Lazarević, Miloš, Nikolić, Nadja, Milašin, Jelena, Miličić, Biljana, "Comparison of four different suture materials in respect to oral wound healing, microbial colonization, tissue reaction and clinical features-randomized clinical study" in Clinical Oral Investigations, 24, no. 4 (2020):1527-1541,
https://doi.org/10.1007/s00784-019-03034-4 . .

TY  - JOUR
AU  - Čarkić, Jelena
AU  - Nikolić, Nadja
AU  - Nišević, Jelena
AU  - Lazarević, Miloš
AU  - Kuzmanović-Pfićer, Jovana
AU  - Jelovac, Drago
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2567
AB  - Oral carcinoma is the sixth most common malignancy worldwide, with survival rates of approximately 50%. The major type of oral cancer, present in 90% of the cases, is oral squamous cell carcinoma (OSCC). The genetic background predisposing an individual to OSCC is complex and largely unknown. Studies have suggested that endothelial nitric oxide synthase (eNOS) gene polymorphisms modulate the cancer risk, prompting us to assess the impact of three functional eNOS gene polymorphisms on OSCC risk. The present study included 50 patients with OSCC and 110 controls. Polymerase chain reaction and restriction fragment length polymorphism analysis were used for genotyping of single-nucleotide polymorphisms -786 T/C (rs2070744) and 894 G/T (rs1799983) and variable number of tandem repeats (VNTR) intron 4b/a polymorphism. Homozygous carriers of -786 T/C and intron 4b/a VNTR variant alleles paired with a significant increase of oral cancer risk [odds ratio (OR): 3.63, 95% confidence interval (CI): 1.08-12.21; P = 0.045 and OR: 11.29, 95% CI: 2.71-47.11; P < 0.001, respectively]. When combined, CC and 4b4a genotypes together led to a 21-fold OSCC risk increase (OR: 21, 95% CI: 2.07-213.29; P = 0.006). Haplotype analysis showed that the C-G-4b haplotype conferred an 11-fold increase in OSCC risk. In conclusion, eNOS polymorphisms considerably influence levels of OSCC risk in the Serbian population.
PB  - Nihon University, School of Dentistry, Japan
T2  - Journal of Oral Science
T1  - Endothelial nitric oxide synthase polymorphisms/haplotypes are strong modulators of oral cancer risk in Serbian population
VL  - 62
IS  - 3
SP  - 322
EP  - 326
DO  - 10.2334/josnusd.19-0310
ER  - 

@article{
author = "Čarkić, Jelena and Nikolić, Nadja and Nišević, Jelena and Lazarević, Miloš and Kuzmanović-Pfićer, Jovana and Jelovac, Drago and Milašin, Jelena",
year = "2020",
abstract = "Oral carcinoma is the sixth most common malignancy worldwide, with survival rates of approximately 50%. The major type of oral cancer, present in 90% of the cases, is oral squamous cell carcinoma (OSCC). The genetic background predisposing an individual to OSCC is complex and largely unknown. Studies have suggested that endothelial nitric oxide synthase (eNOS) gene polymorphisms modulate the cancer risk, prompting us to assess the impact of three functional eNOS gene polymorphisms on OSCC risk. The present study included 50 patients with OSCC and 110 controls. Polymerase chain reaction and restriction fragment length polymorphism analysis were used for genotyping of single-nucleotide polymorphisms -786 T/C (rs2070744) and 894 G/T (rs1799983) and variable number of tandem repeats (VNTR) intron 4b/a polymorphism. Homozygous carriers of -786 T/C and intron 4b/a VNTR variant alleles paired with a significant increase of oral cancer risk [odds ratio (OR): 3.63, 95% confidence interval (CI): 1.08-12.21; P = 0.045 and OR: 11.29, 95% CI: 2.71-47.11; P < 0.001, respectively]. When combined, CC and 4b4a genotypes together led to a 21-fold OSCC risk increase (OR: 21, 95% CI: 2.07-213.29; P = 0.006). Haplotype analysis showed that the C-G-4b haplotype conferred an 11-fold increase in OSCC risk. In conclusion, eNOS polymorphisms considerably influence levels of OSCC risk in the Serbian population.",
publisher = "Nihon University, School of Dentistry, Japan",
journal = "Journal of Oral Science",
title = "Endothelial nitric oxide synthase polymorphisms/haplotypes are strong modulators of oral cancer risk in Serbian population",
volume = "62",
number = "3",
pages = "322-326",
doi = "10.2334/josnusd.19-0310"
}

Čarkić, J., Nikolić, N., Nišević, J., Lazarević, M., Kuzmanović-Pfićer, J., Jelovac, D.,& Milašin, J.. (2020). Endothelial nitric oxide synthase polymorphisms/haplotypes are strong modulators of oral cancer risk in Serbian population. in Journal of Oral Science
Nihon University, School of Dentistry, Japan., 62(3), 322-326.
https://doi.org/10.2334/josnusd.19-0310

Čarkić J, Nikolić N, Nišević J, Lazarević M, Kuzmanović-Pfićer J, Jelovac D, Milašin J. Endothelial nitric oxide synthase polymorphisms/haplotypes are strong modulators of oral cancer risk in Serbian population. in Journal of Oral Science. 2020;62(3):322-326.
doi:10.2334/josnusd.19-0310 .

Čarkić, Jelena, Nikolić, Nadja, Nišević, Jelena, Lazarević, Miloš, Kuzmanović-Pfićer, Jovana, Jelovac, Drago, Milašin, Jelena, "Endothelial nitric oxide synthase polymorphisms/haplotypes are strong modulators of oral cancer risk in Serbian population" in Journal of Oral Science, 62, no. 3 (2020):322-326,
https://doi.org/10.2334/josnusd.19-0310 . .

TY  - JOUR
AU  - Šljivančanin Jakovljević, Tamara
AU  - Kontić-Vučinić, Olivera
AU  - Nikolić, Nadja
AU  - Čarkić, Jelena
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2572
AB  - Objectives: Establishment of association between: (a) Val158Met COMT (G1947A) polymorphism and preeclampsia; (b) cytokines gene expression and COMT genotypes. Methods: 50 preeclampsia and 50 healthy pregnant women were enrolled. COMT genotyping was done by PCR/RFLP. TNF-α, IL-1β, and IL-6 mRNA levels were determined by Real-time PCR. Results: Variant (AA) homozygotes carried 3.7-fold increased preeclampsia odds, especially for severe (OR = 9.0, 95%CI (2.09-38.799)) and early forms (OR = 6.6, 95%CI (1.62-26.87)). AA homozygotes with PE had higher TNF-α levels compared to controls (P = 0.012). Conclusions: Val158Met COMT polymorphism increases preeclampsia risk. TNF-α expression and Val158Met COMT polymorphism have concomitant roles in PE pathogenesis.
PB  - Taylor & Francis Inc.
T2  - Hypertension in Pregnancy
T1  - VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia
VL  - 39
IS  - 4
SP  - 471
EP  - 480
DO  - 10.1080/10641955.2020.1843663
ER  - 

@article{
author = "Šljivančanin Jakovljević, Tamara and Kontić-Vučinić, Olivera and Nikolić, Nadja and Čarkić, Jelena and Milašin, Jelena",
year = "2020",
abstract = "Objectives: Establishment of association between: (a) Val158Met COMT (G1947A) polymorphism and preeclampsia; (b) cytokines gene expression and COMT genotypes. Methods: 50 preeclampsia and 50 healthy pregnant women were enrolled. COMT genotyping was done by PCR/RFLP. TNF-α, IL-1β, and IL-6 mRNA levels were determined by Real-time PCR. Results: Variant (AA) homozygotes carried 3.7-fold increased preeclampsia odds, especially for severe (OR = 9.0, 95%CI (2.09-38.799)) and early forms (OR = 6.6, 95%CI (1.62-26.87)). AA homozygotes with PE had higher TNF-α levels compared to controls (P = 0.012). Conclusions: Val158Met COMT polymorphism increases preeclampsia risk. TNF-α expression and Val158Met COMT polymorphism have concomitant roles in PE pathogenesis.",
publisher = "Taylor & Francis Inc.",
journal = "Hypertension in Pregnancy",
title = "VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia",
volume = "39",
number = "4",
pages = "471-480",
doi = "10.1080/10641955.2020.1843663"
}

Šljivančanin Jakovljević, T., Kontić-Vučinić, O., Nikolić, N., Čarkić, J.,& Milašin, J.. (2020). VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia. in Hypertension in Pregnancy
Taylor & Francis Inc.., 39(4), 471-480.
https://doi.org/10.1080/10641955.2020.1843663

Šljivančanin Jakovljević T, Kontić-Vučinić O, Nikolić N, Čarkić J, Milašin J. VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia. in Hypertension in Pregnancy. 2020;39(4):471-480.
doi:10.1080/10641955.2020.1843663 .

Šljivančanin Jakovljević, Tamara, Kontić-Vučinić, Olivera, Nikolić, Nadja, Čarkić, Jelena, Milašin, Jelena, "VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia" in Hypertension in Pregnancy, 39, no. 4 (2020):471-480,
https://doi.org/10.1080/10641955.2020.1843663 . .

TY  - JOUR
AU  - Matić Petrović, Sanja
AU  - Nikolić, Nadja
AU  - Toljić, Boško
AU  - Arambašić-Jovanović, Jelena
AU  - Miličić, Biljana
AU  - Miličić, Tanja
AU  - Jotić, Aleksandra
AU  - Vidaković, Melita
AU  - Milašin, Jelena
AU  - Pucar, Ana
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2571
AB  - Objectives: Aiming to show that periodontitis (PD) and type 2 diabetes (T2D) are bidirectionally related and potentially linked by inflammatory cytokines, we searched for association between -308 G/A Tumor necrosis factor-alpha (TNFα), +252A/G Lymphotoxin-alpha (LTα), +36A/G Tumor necrosis factor receptor 1 (TNFR1) and +676 T/G tumor necrosis factor receptor 2 (TNFR2) single nucleotide polymorphisms (SNPs) and: risk of PD or PD + T2D; periodontitis parameters in PD and PD + T2D; serum levels of cytokines/their receptors. Relationship between periodontal inflammation and serum cytokine/receptor levels was also assessed.

Design: Subjects were stratified as: 57 healthy controls (HC); 58 PD; 65 PD + T2D. Sociodemographic, environmental, behavioral and periodontal clinical data were recorded. SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism, while cytokines/receptors levels were quantified by enzyme-linked immunosorbent assay. Impact of periodontal inflammation was measured using periodontal inflamed surface area (PISA).

Results: TNFα AA genotype showed protective effect in T2D + PD compared to PD, even adjusted for behavioral/environmental factors (OR 0.18; 95 %CI 0.037-0.886; p = 0.035). LTα AG heterozygotes had increased risk of PD (OR 3.27; 95 %CI 1.35-7.96; p = 0.016), while TNFR2 TG genotype had protective effect (OR = 0.44; 95 %CI 0.954-0.9794; p = 0.043). TNFR1 AA was predictor of periodontal pocket depth and clinical attachment loss in PD. Correlation between TNFR2 concentration and PISA was negative in PD, positive in PD + T2D.

Conclusions: None of the SNPs showed cross-susceptibility between PD and T2D. + 252A/G LTα and +676 T/G TNFR2 SNPs are associated with PD risk. Periodontal destruction in healthy individuals is influenced by TNFR1 genotype. Impact of periodontal on systemic inflammation is masked by T2D.
PB  - Elsevier Inc.
T2  - Archives of Oral Biology
T1  - The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population
VL  - 120
SP  - 104929
DO  - 10.1016/j.archoralbio.2020.104929
ER  - 

@article{
author = "Matić Petrović, Sanja and Nikolić, Nadja and Toljić, Boško and Arambašić-Jovanović, Jelena and Miličić, Biljana and Miličić, Tanja and Jotić, Aleksandra and Vidaković, Melita and Milašin, Jelena and Pucar, Ana",
year = "2020",
abstract = "Objectives: Aiming to show that periodontitis (PD) and type 2 diabetes (T2D) are bidirectionally related and potentially linked by inflammatory cytokines, we searched for association between -308 G/A Tumor necrosis factor-alpha (TNFα), +252A/G Lymphotoxin-alpha (LTα), +36A/G Tumor necrosis factor receptor 1 (TNFR1) and +676 T/G tumor necrosis factor receptor 2 (TNFR2) single nucleotide polymorphisms (SNPs) and: risk of PD or PD + T2D; periodontitis parameters in PD and PD + T2D; serum levels of cytokines/their receptors. Relationship between periodontal inflammation and serum cytokine/receptor levels was also assessed.

Design: Subjects were stratified as: 57 healthy controls (HC); 58 PD; 65 PD + T2D. Sociodemographic, environmental, behavioral and periodontal clinical data were recorded. SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism, while cytokines/receptors levels were quantified by enzyme-linked immunosorbent assay. Impact of periodontal inflammation was measured using periodontal inflamed surface area (PISA).

Results: TNFα AA genotype showed protective effect in T2D + PD compared to PD, even adjusted for behavioral/environmental factors (OR 0.18; 95 %CI 0.037-0.886; p = 0.035). LTα AG heterozygotes had increased risk of PD (OR 3.27; 95 %CI 1.35-7.96; p = 0.016), while TNFR2 TG genotype had protective effect (OR = 0.44; 95 %CI 0.954-0.9794; p = 0.043). TNFR1 AA was predictor of periodontal pocket depth and clinical attachment loss in PD. Correlation between TNFR2 concentration and PISA was negative in PD, positive in PD + T2D.

Conclusions: None of the SNPs showed cross-susceptibility between PD and T2D. + 252A/G LTα and +676 T/G TNFR2 SNPs are associated with PD risk. Periodontal destruction in healthy individuals is influenced by TNFR1 genotype. Impact of periodontal on systemic inflammation is masked by T2D.",
publisher = "Elsevier Inc.",
journal = "Archives of Oral Biology",
title = "The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population",
volume = "120",
pages = "104929",
doi = "10.1016/j.archoralbio.2020.104929"
}

Matić Petrović, S., Nikolić, N., Toljić, B., Arambašić-Jovanović, J., Miličić, B., Miličić, T., Jotić, A., Vidaković, M., Milašin, J.,& Pucar, A.. (2020). The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population. in Archives of Oral Biology
Elsevier Inc.., 120, 104929.
https://doi.org/10.1016/j.archoralbio.2020.104929

Matić Petrović S, Nikolić N, Toljić B, Arambašić-Jovanović J, Miličić B, Miličić T, Jotić A, Vidaković M, Milašin J, Pucar A. The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population. in Archives of Oral Biology. 2020;120:104929.
doi:10.1016/j.archoralbio.2020.104929 .

Matić Petrović, Sanja, Nikolić, Nadja, Toljić, Boško, Arambašić-Jovanović, Jelena, Miličić, Biljana, Miličić, Tanja, Jotić, Aleksandra, Vidaković, Melita, Milašin, Jelena, Pucar, Ana, "The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population" in Archives of Oral Biology, 120 (2020):104929,
https://doi.org/10.1016/j.archoralbio.2020.104929 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Nikolić, Nadja
AU  - Jaćimović, Jelena
AU  - Pavlović, Ognjan
AU  - Miličić, Biljana
AU  - Beljić-Ivanović, Katarina
AU  - Miletić, Maja
AU  - Andrić, Miroslav
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2570
AB  - Introduction
 This study aimed to summarize data on apical periodontitis (AP) and nonsurgical root canal treatment (NSRCT) prevalence and risk factors related to age, gender, and quality of restorative and endodontic treatment in the general population from cross-sectional studies published between 2012 and 2020.
 
 Methods
 An electronic search was performed in the following databases: Web of Science, Scopus, and PubMed. The conducted literature search covered studies published between 2012 and 2020, without restrictions on language. The STROBE and NOS tools were used for quality assessment of the included studies.
 
 Results
 Sixteen articles were included in the review. In total, 200,041 teeth were examined. On average, 6.3% of teeth had AP, and 7.4% had NSRCT. Forty-one percent of RCT teeth had AP, and 3.5% of untreated teeth had AP. Female patients were less prone to AP in endodontically treated teeth only, compared with male patients (P < .001). Variable stratification of age subgroups among included studies prevented us from conducting a meta-analysis. An increase in AP frequency was found in teeth with inadequate restorative and endodontic treatment (P < .001 and P < .001, respectively). Because of high heterogeneity, these results should be taken with caution.
 
 Conclusions
 There is an increased AP prevalence in the adult general population compared with data from 2012 (6.3% versus 5.4%) in both endodontically treated (41.3% versus 35.9%) and untreated teeth (3.5% versus 2.1%). In addition, AP developed less frequently in female patients with endodontically treated teeth and in teeth with inadequate compared with adequate restorative and endodontic treatment.
PB  - Elsevier Inc.
T2  - Journal of Endodontics
T1  - Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020
VL  - 46
IS  - 10
SP  - 1371
EP  - 1386.e8
DO  - 10.1016/j.joen.2020.07.007
ER  - 

@article{
author = "Jakovljević, Aleksandar and Nikolić, Nadja and Jaćimović, Jelena and Pavlović, Ognjan and Miličić, Biljana and Beljić-Ivanović, Katarina and Miletić, Maja and Andrić, Miroslav and Milašin, Jelena",
year = "2020",
abstract = "Introduction
 This study aimed to summarize data on apical periodontitis (AP) and nonsurgical root canal treatment (NSRCT) prevalence and risk factors related to age, gender, and quality of restorative and endodontic treatment in the general population from cross-sectional studies published between 2012 and 2020.
 
 Methods
 An electronic search was performed in the following databases: Web of Science, Scopus, and PubMed. The conducted literature search covered studies published between 2012 and 2020, without restrictions on language. The STROBE and NOS tools were used for quality assessment of the included studies.
 
 Results
 Sixteen articles were included in the review. In total, 200,041 teeth were examined. On average, 6.3% of teeth had AP, and 7.4% had NSRCT. Forty-one percent of RCT teeth had AP, and 3.5% of untreated teeth had AP. Female patients were less prone to AP in endodontically treated teeth only, compared with male patients (P < .001). Variable stratification of age subgroups among included studies prevented us from conducting a meta-analysis. An increase in AP frequency was found in teeth with inadequate restorative and endodontic treatment (P < .001 and P < .001, respectively). Because of high heterogeneity, these results should be taken with caution.
 
 Conclusions
 There is an increased AP prevalence in the adult general population compared with data from 2012 (6.3% versus 5.4%) in both endodontically treated (41.3% versus 35.9%) and untreated teeth (3.5% versus 2.1%). In addition, AP developed less frequently in female patients with endodontically treated teeth and in teeth with inadequate compared with adequate restorative and endodontic treatment.",
publisher = "Elsevier Inc.",
journal = "Journal of Endodontics",
title = "Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020",
volume = "46",
number = "10",
pages = "1371-1386.e8",
doi = "10.1016/j.joen.2020.07.007"
}

Jakovljević, A., Nikolić, N., Jaćimović, J., Pavlović, O., Miličić, B., Beljić-Ivanović, K., Miletić, M., Andrić, M.,& Milašin, J.. (2020). Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020. in Journal of Endodontics
Elsevier Inc.., 46(10), 1371-1386.e8.
https://doi.org/10.1016/j.joen.2020.07.007

Jakovljević A, Nikolić N, Jaćimović J, Pavlović O, Miličić B, Beljić-Ivanović K, Miletić M, Andrić M, Milašin J. Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020. in Journal of Endodontics. 2020;46(10):1371-1386.e8.
doi:10.1016/j.joen.2020.07.007 .

Jakovljević, Aleksandar, Nikolić, Nadja, Jaćimović, Jelena, Pavlović, Ognjan, Miličić, Biljana, Beljić-Ivanović, Katarina, Miletić, Maja, Andrić, Miroslav, Milašin, Jelena, "Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020" in Journal of Endodontics, 46, no. 10 (2020):1371-1386.e8,
https://doi.org/10.1016/j.joen.2020.07.007 . .

TY  - JOUR
AU  - Šljivančanin Jakovljević, Tamara
AU  - Jaćimović, Jelena
AU  - Nikolić, Nadja
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2569
AB  - Insufficient response to oxidative stress in placenta is proposed as a contributing factor for preeclampsia (PE) development. Glutathione S-transferases (GST) have significant role in detoxification processes. Conflicting results were published by several research groups regarding GST T1 and GST M1 deletion polymorphism as risk factors for PE. The aim of the present meta-analysis was to get a better understanding of the impact of these polymorphisms in preeclampsia development. To identify relevant case-control studies, the author team searched Clarivate Analytics Web of Science, Scopus, PubMed, Cochrane Central Register of Controlled Trials, China National Knowledge Infrastructure, major subject journals, and gray literature. Pooled odds ratios and 95% confidence intervals for GST M1 and GST T1 deletion polymorphism and preeclampsia were derived from random effects models. This meta-analysis included 10 eligible studies. The pooled analyses showed no association between GST M1/GST T1 deletion polymorphisms and susceptibility to PE. Even though high heterogeneity was founded among results for GST M1 and double null genotypes, Egger's and Begg's tests (0.17 and 0.18, respectively) revealed no statistical evidence of publication bias among included studies. The present updated systematic review and meta-analysis found no association between GST M1 and GST T1 deletion polymorphism and PE risk.
PB  - Wiley
T2  - American Journal of Reproductive Immunology
T1  - Lack of association between glutathione S-transferase M1 and T1 gene polymorphisms and susceptibility to preeclampsia: An updated systematic review and meta-analysis
VL  - 84
IS  - 6
SP  - e13303
DO  - 10.1111/aji.13303
ER  - 

@article{
author = "Šljivančanin Jakovljević, Tamara and Jaćimović, Jelena and Nikolić, Nadja and Milašin, Jelena",
year = "2020",
abstract = "Insufficient response to oxidative stress in placenta is proposed as a contributing factor for preeclampsia (PE) development. Glutathione S-transferases (GST) have significant role in detoxification processes. Conflicting results were published by several research groups regarding GST T1 and GST M1 deletion polymorphism as risk factors for PE. The aim of the present meta-analysis was to get a better understanding of the impact of these polymorphisms in preeclampsia development. To identify relevant case-control studies, the author team searched Clarivate Analytics Web of Science, Scopus, PubMed, Cochrane Central Register of Controlled Trials, China National Knowledge Infrastructure, major subject journals, and gray literature. Pooled odds ratios and 95% confidence intervals for GST M1 and GST T1 deletion polymorphism and preeclampsia were derived from random effects models. This meta-analysis included 10 eligible studies. The pooled analyses showed no association between GST M1/GST T1 deletion polymorphisms and susceptibility to PE. Even though high heterogeneity was founded among results for GST M1 and double null genotypes, Egger's and Begg's tests (0.17 and 0.18, respectively) revealed no statistical evidence of publication bias among included studies. The present updated systematic review and meta-analysis found no association between GST M1 and GST T1 deletion polymorphism and PE risk.",
publisher = "Wiley",
journal = "American Journal of Reproductive Immunology",
title = "Lack of association between glutathione S-transferase M1 and T1 gene polymorphisms and susceptibility to preeclampsia: An updated systematic review and meta-analysis",
volume = "84",
number = "6",
pages = "e13303",
doi = "10.1111/aji.13303"
}

Šljivančanin Jakovljević, T., Jaćimović, J., Nikolić, N.,& Milašin, J.. (2020). Lack of association between glutathione S-transferase M1 and T1 gene polymorphisms and susceptibility to preeclampsia: An updated systematic review and meta-analysis. in American Journal of Reproductive Immunology
Wiley., 84(6), e13303.
https://doi.org/10.1111/aji.13303

Šljivančanin Jakovljević T, Jaćimović J, Nikolić N, Milašin J. Lack of association between glutathione S-transferase M1 and T1 gene polymorphisms and susceptibility to preeclampsia: An updated systematic review and meta-analysis. in American Journal of Reproductive Immunology. 2020;84(6):e13303.
doi:10.1111/aji.13303 .

Šljivančanin Jakovljević, Tamara, Jaćimović, Jelena, Nikolić, Nadja, Milašin, Jelena, "Lack of association between glutathione S-transferase M1 and T1 gene polymorphisms and susceptibility to preeclampsia: An updated systematic review and meta-analysis" in American Journal of Reproductive Immunology, 84, no. 6 (2020):e13303,
https://doi.org/10.1111/aji.13303 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Nikolić, Nadja
AU  - Jaćimović, Jelena
AU  - Pavlović, Ognjan
AU  - Miličić, Biljana
AU  - Beljić-Ivanović, Katarina
AU  - Miletić, Maja
AU  - Andrić, Miroslav
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2531
AB  - Introduction
This study aimed to summarize data on apical periodontitis (AP) and nonsurgical root canal treatment (NSRCT) prevalence and risk factors related to age, gender, and quality of restorative and endodontic treatment in the general population from cross-sectional studies published between 2012 and 2020.

Methods
An electronic search was performed in the following databases: Web of Science, Scopus, and PubMed. The conducted literature search covered studies published between 2012 and 2020, without restrictions on language. The STROBE and NOS tools were used for quality assessment of the included studies.

Results
Sixteen articles were included in the review. In total, 200,041 teeth were examined. On average, 6.3% of teeth had AP, and 7.4% had NSRCT. Forty-one percent of RCT teeth had AP, and 3.5% of untreated teeth had AP. Female patients were less prone to AP in endodontically treated teeth only, compared with male patients (P < .001). Variable stratification of age subgroups among included studies prevented us from conducting a meta-analysis. An increase in AP frequency was found in teeth with inadequate restorative and endodontic treatment (P < .001 and P < .001, respectively). Because of high heterogeneity, these results should be taken with caution.

Conclusions
There is an increased AP prevalence in the adult general population compared with data from 2012 (6.3% versus 5.4%) in both endodontically treated (41.3% versus 35.9%) and untreated teeth (3.5% versus 2.1%). In addition, AP developed less frequently in female patients with endodontically treated teeth and in teeth with inadequate compared with adequate restorative and endodontic treatment.
PB  - Elsevier Inc.
T2  - Journal of Endodontics
T1  - Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020
VL  - 46
IS  - 10
SP  - 1371
EP  - 1386
DO  - 10.1016/j.joen.2020.07.007
ER  - 

@article{
author = "Jakovljević, Aleksandar and Nikolić, Nadja and Jaćimović, Jelena and Pavlović, Ognjan and Miličić, Biljana and Beljić-Ivanović, Katarina and Miletić, Maja and Andrić, Miroslav and Milašin, Jelena",
year = "2020",
abstract = "Introduction
This study aimed to summarize data on apical periodontitis (AP) and nonsurgical root canal treatment (NSRCT) prevalence and risk factors related to age, gender, and quality of restorative and endodontic treatment in the general population from cross-sectional studies published between 2012 and 2020.

Methods
An electronic search was performed in the following databases: Web of Science, Scopus, and PubMed. The conducted literature search covered studies published between 2012 and 2020, without restrictions on language. The STROBE and NOS tools were used for quality assessment of the included studies.

Results
Sixteen articles were included in the review. In total, 200,041 teeth were examined. On average, 6.3% of teeth had AP, and 7.4% had NSRCT. Forty-one percent of RCT teeth had AP, and 3.5% of untreated teeth had AP. Female patients were less prone to AP in endodontically treated teeth only, compared with male patients (P < .001). Variable stratification of age subgroups among included studies prevented us from conducting a meta-analysis. An increase in AP frequency was found in teeth with inadequate restorative and endodontic treatment (P < .001 and P < .001, respectively). Because of high heterogeneity, these results should be taken with caution.

Conclusions
There is an increased AP prevalence in the adult general population compared with data from 2012 (6.3% versus 5.4%) in both endodontically treated (41.3% versus 35.9%) and untreated teeth (3.5% versus 2.1%). In addition, AP developed less frequently in female patients with endodontically treated teeth and in teeth with inadequate compared with adequate restorative and endodontic treatment.",
publisher = "Elsevier Inc.",
journal = "Journal of Endodontics",
title = "Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020",
volume = "46",
number = "10",
pages = "1371-1386",
doi = "10.1016/j.joen.2020.07.007"
}

Jakovljević, A., Nikolić, N., Jaćimović, J., Pavlović, O., Miličić, B., Beljić-Ivanović, K., Miletić, M., Andrić, M.,& Milašin, J.. (2020). Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020. in Journal of Endodontics
Elsevier Inc.., 46(10), 1371-1386.
https://doi.org/10.1016/j.joen.2020.07.007

Jakovljević A, Nikolić N, Jaćimović J, Pavlović O, Miličić B, Beljić-Ivanović K, Miletić M, Andrić M, Milašin J. Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020. in Journal of Endodontics. 2020;46(10):1371-1386.
doi:10.1016/j.joen.2020.07.007 .

Jakovljević, Aleksandar, Nikolić, Nadja, Jaćimović, Jelena, Pavlović, Ognjan, Miličić, Biljana, Beljić-Ivanović, Katarina, Miletić, Maja, Andrić, Miroslav, Milašin, Jelena, "Prevalence of Apical Periodontitis and Conventional Nonsurgical Root Canal Treatment in General Adult Population: An Updated Systematic Review and Meta-analysis of Cross-sectional Studies Published between 2012 and 2020" in Journal of Endodontics, 46, no. 10 (2020):1371-1386,
https://doi.org/10.1016/j.joen.2020.07.007 . .

TY  - JOUR
AU  - Vučković, Mila
AU  - Nikolić, Nadja
AU  - Milašin, Jelena
AU  - Đorđević, Vladan
AU  - Milinković, Iva
AU  - Asotić, Jasminka
AU  - Jezdić, Zoran
AU  - Janković, Saša
AU  - Aleksić, Zoran
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2525
AB  - Introduction/Objective. The objective of the study was to investigate whether there are differences in therapeutic effect between initial treatments of chronic periodontitis [scaling and root planning (SRP)] alone and SRP in conjunction with injectable platelet-rich fibrin (I-PRF) application, comparing clinical parameters after three months. Methods. Twenty-four patients with chronic periodontitis who had at least two sites with probing pocket depth (PPD) ≥ 5 mm on contralateral side participated in the study. Using a split-mouth design, the patients were treated with SRP + I-PRF (study group) or SRP only (control group). The clinical parameters, clinical attachment level (CAL), gingival margin level (GML), PPD, bleeding on probing, and plaque index, were recorded on both sides. Results. Compared to baseline, both treatment modalities demonstrated an improvement in investigated clinical parameters. The mean value of CAL was reduced from 1.97 ± 0.75 (0.25–3.31) to 1.07 ± 0.44 (0.12–1.78) in the study group, whereas it decreased from 1.81 ± 0.66 (0.42–2.96) to 1.48 ± 0.55 (0.22–2.30) in the control group. Similarly, the corresponding values for GML and PPD showed statistically significant difference between the groups (p = 0.040 and p = 0.006, respectively). Conclusion. Regardless the limited number of patients in the study, initial periodontal therapy in conjunction with injectable platelet-rich fibrin proved to display significant improvement in all clinical parameters compared to initial periodontal therapy alone.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - The effect of injectable platelet-rich fibrin use in the initial treatment of chronic periodontitis
VL  - 148
IS  - 5-6
SP  - 280
EP  - 285
DO  - 10.2298/SARH190925022V
ER  - 

@article{
author = "Vučković, Mila and Nikolić, Nadja and Milašin, Jelena and Đorđević, Vladan and Milinković, Iva and Asotić, Jasminka and Jezdić, Zoran and Janković, Saša and Aleksić, Zoran",
year = "2020",
abstract = "Introduction/Objective. The objective of the study was to investigate whether there are differences in therapeutic effect between initial treatments of chronic periodontitis [scaling and root planning (SRP)] alone and SRP in conjunction with injectable platelet-rich fibrin (I-PRF) application, comparing clinical parameters after three months. Methods. Twenty-four patients with chronic periodontitis who had at least two sites with probing pocket depth (PPD) ≥ 5 mm on contralateral side participated in the study. Using a split-mouth design, the patients were treated with SRP + I-PRF (study group) or SRP only (control group). The clinical parameters, clinical attachment level (CAL), gingival margin level (GML), PPD, bleeding on probing, and plaque index, were recorded on both sides. Results. Compared to baseline, both treatment modalities demonstrated an improvement in investigated clinical parameters. The mean value of CAL was reduced from 1.97 ± 0.75 (0.25–3.31) to 1.07 ± 0.44 (0.12–1.78) in the study group, whereas it decreased from 1.81 ± 0.66 (0.42–2.96) to 1.48 ± 0.55 (0.22–2.30) in the control group. Similarly, the corresponding values for GML and PPD showed statistically significant difference between the groups (p = 0.040 and p = 0.006, respectively). Conclusion. Regardless the limited number of patients in the study, initial periodontal therapy in conjunction with injectable platelet-rich fibrin proved to display significant improvement in all clinical parameters compared to initial periodontal therapy alone.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "The effect of injectable platelet-rich fibrin use in the initial treatment of chronic periodontitis",
volume = "148",
number = "5-6",
pages = "280-285",
doi = "10.2298/SARH190925022V"
}

Vučković, M., Nikolić, N., Milašin, J., Đorđević, V., Milinković, I., Asotić, J., Jezdić, Z., Janković, S.,& Aleksić, Z.. (2020). The effect of injectable platelet-rich fibrin use in the initial treatment of chronic periodontitis. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 148(5-6), 280-285.
https://doi.org/10.2298/SARH190925022V

Vučković M, Nikolić N, Milašin J, Đorđević V, Milinković I, Asotić J, Jezdić Z, Janković S, Aleksić Z. The effect of injectable platelet-rich fibrin use in the initial treatment of chronic periodontitis. in Srpski arhiv za celokupno lekarstvo. 2020;148(5-6):280-285.
doi:10.2298/SARH190925022V .

Vučković, Mila, Nikolić, Nadja, Milašin, Jelena, Đorđević, Vladan, Milinković, Iva, Asotić, Jasminka, Jezdić, Zoran, Janković, Saša, Aleksić, Zoran, "The effect of injectable platelet-rich fibrin use in the initial treatment of chronic periodontitis" in Srpski arhiv za celokupno lekarstvo, 148, no. 5-6 (2020):280-285,
https://doi.org/10.2298/SARH190925022V . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Nikolić, Nadja
AU  - Čarkić, Jelena
AU  - Andrić, Miroslav
AU  - Miletić, Maja
AU  - Beljić-Ivanović, Katarina
AU  - Jovanović, Tanja
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2493
AB  - Objectives: This study aimed to investigate whether Epstein-Barr virus (EBV) positive periapical lesions exhibited higher mRNA levels of Notch signalling molecules (Notch2 and Jagged1), bone resorption regulators (receptor activator of nuclear factor kappa-? ligand (RANKL) and osteoprotegerin (OPG)), and proinflammatory cytokines (tumour necrosis factor-alpha (TNF-?), interleukin-1 beta (IL-1?) and IL-6) compared to EBV negative lesions. Additionally, the potential correlation between investigated molecules in periapical lesions was analyzed. Materials and methods: Sixty-four apical periodontitis lesions were obtained subsequent to standard apicoectomy procedure. The presence of EBV was determined using nested PCR. Based on the presence of EBV all periapical lesions were divided into two groups, 29 EBV positive and 35 EBV negative lesions. A reverse transcriptase real-time PCR was used to determine mRNA levels of Notch2, Jagged1, RANKL, OPG, TNF-?, IL-1? and IL-6. Results: Significantly higher mRNA levels of Notch2, Jagged1, RANKL and IL-1? were observed in EBV positive compared to EBV negative lesions. Significant positive correlation was present between Notch2 and Jagged1, Jagged1 and RANKL, and IL-? and TNF-? in EBV positive periapical lesions. Conclusions: Notch signalling pathway may be involved in alveolar bone resorption in apical periodontitis lesions infected by EBV.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Acta Odontologica Scandinavica
T1  - Notch ? a possible mediator between Epstein-Barr virus infection and bone resorption in apical periodontitis
VL  - 78
IS  - 2
SP  - 126
EP  - 131
DO  - 10.1080/00016357.2019.1658896
ER  - 

@article{
author = "Jakovljević, Aleksandar and Nikolić, Nadja and Čarkić, Jelena and Andrić, Miroslav and Miletić, Maja and Beljić-Ivanović, Katarina and Jovanović, Tanja and Milašin, Jelena",
year = "2020",
abstract = "Objectives: This study aimed to investigate whether Epstein-Barr virus (EBV) positive periapical lesions exhibited higher mRNA levels of Notch signalling molecules (Notch2 and Jagged1), bone resorption regulators (receptor activator of nuclear factor kappa-? ligand (RANKL) and osteoprotegerin (OPG)), and proinflammatory cytokines (tumour necrosis factor-alpha (TNF-?), interleukin-1 beta (IL-1?) and IL-6) compared to EBV negative lesions. Additionally, the potential correlation between investigated molecules in periapical lesions was analyzed. Materials and methods: Sixty-four apical periodontitis lesions were obtained subsequent to standard apicoectomy procedure. The presence of EBV was determined using nested PCR. Based on the presence of EBV all periapical lesions were divided into two groups, 29 EBV positive and 35 EBV negative lesions. A reverse transcriptase real-time PCR was used to determine mRNA levels of Notch2, Jagged1, RANKL, OPG, TNF-?, IL-1? and IL-6. Results: Significantly higher mRNA levels of Notch2, Jagged1, RANKL and IL-1? were observed in EBV positive compared to EBV negative lesions. Significant positive correlation was present between Notch2 and Jagged1, Jagged1 and RANKL, and IL-? and TNF-? in EBV positive periapical lesions. Conclusions: Notch signalling pathway may be involved in alveolar bone resorption in apical periodontitis lesions infected by EBV.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Acta Odontologica Scandinavica",
title = "Notch ? a possible mediator between Epstein-Barr virus infection and bone resorption in apical periodontitis",
volume = "78",
number = "2",
pages = "126-131",
doi = "10.1080/00016357.2019.1658896"
}

Jakovljević, A., Nikolić, N., Čarkić, J., Andrić, M., Miletić, M., Beljić-Ivanović, K., Jovanović, T.,& Milašin, J.. (2020). Notch ? a possible mediator between Epstein-Barr virus infection and bone resorption in apical periodontitis. in Acta Odontologica Scandinavica
Taylor & Francis Ltd, Abingdon., 78(2), 126-131.
https://doi.org/10.1080/00016357.2019.1658896

Jakovljević A, Nikolić N, Čarkić J, Andrić M, Miletić M, Beljić-Ivanović K, Jovanović T, Milašin J. Notch ? a possible mediator between Epstein-Barr virus infection and bone resorption in apical periodontitis. in Acta Odontologica Scandinavica. 2020;78(2):126-131.
doi:10.1080/00016357.2019.1658896 .

Jakovljević, Aleksandar, Nikolić, Nadja, Čarkić, Jelena, Andrić, Miroslav, Miletić, Maja, Beljić-Ivanović, Katarina, Jovanović, Tanja, Milašin, Jelena, "Notch ? a possible mediator between Epstein-Barr virus infection and bone resorption in apical periodontitis" in Acta Odontologica Scandinavica, 78, no. 2 (2020):126-131,
https://doi.org/10.1080/00016357.2019.1658896 . .

TY  - JOUR
AU  - Mijailović, Iva
AU  - Nikolić, Nadja
AU  - Đinić, Ana
AU  - Čarkić, Jelena
AU  - Milinković, Iva
AU  - Perić, Mina
AU  - Janković, Saša
AU  - Milašin, Jelena
AU  - Aleksić, Zoran
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2492
AB  - Background The exact mechanisms of bone resorption in periodontitis have not been fully elucidated. The aims of this study were to analyze the expression of Notch signaling molecules, bone remodeling mediators, and pro-inflammatory cytokines in periodontitis patients and to determine their potential correlations. Methods The study included 130 individuals: 40 with aggressive periodontitis (AP group), 40 with chronic periodontitis (CP group), and 50 periodontally healthy controls. Total RNA was extracted from gingival crevicular fluid samples and relative gene expression of investigated molecules (Notch 1, Notch 2, Jagged 1, Hes 1, Hey 1, TNF-alpha, IL-17, RANKL, and OPG) was determined by reverse transcriptase - real-time polymerase chain reaction (RT-qPCR). Results In AP group, a significant increase of Notch 2, TNF-alpha, IL-17 and RANKL and a significant decrease of Notch 1 and Jagged 1 expression were observed compared to control group (P = 0.023, P = 0.005, P = 0.030, and P = 0.001 P = 0.031 and P = 0.029, respectively). Notch 2 and RANKL were also overexpressed in CP group compared to controls (P = 0.001 and P = 0.011). Significant correlations were observed in AP group between expression levels of the analyzed genes. Conclusion The present findings implicate Notch 2 overexpression in the ethiopathogenesis of bone resorption in aggressive and chronic periodontitis. The down-regulation of Notch 1 and Jagged 1 and loss of their osteoprotective function might cause a more excessive osteoclast formation and contribute to greater osteolysis in aggressive periodontitis.
PB  - Wiley, Hoboken
T2  - Journal of Periodontology
T1  - The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis
VL  - 91
IS  - 4
SP  - 554
EP  - 561
DO  - 10.1002/JPER.18-0755
ER  - 

@article{
author = "Mijailović, Iva and Nikolić, Nadja and Đinić, Ana and Čarkić, Jelena and Milinković, Iva and Perić, Mina and Janković, Saša and Milašin, Jelena and Aleksić, Zoran",
year = "2020",
abstract = "Background The exact mechanisms of bone resorption in periodontitis have not been fully elucidated. The aims of this study were to analyze the expression of Notch signaling molecules, bone remodeling mediators, and pro-inflammatory cytokines in periodontitis patients and to determine their potential correlations. Methods The study included 130 individuals: 40 with aggressive periodontitis (AP group), 40 with chronic periodontitis (CP group), and 50 periodontally healthy controls. Total RNA was extracted from gingival crevicular fluid samples and relative gene expression of investigated molecules (Notch 1, Notch 2, Jagged 1, Hes 1, Hey 1, TNF-alpha, IL-17, RANKL, and OPG) was determined by reverse transcriptase - real-time polymerase chain reaction (RT-qPCR). Results In AP group, a significant increase of Notch 2, TNF-alpha, IL-17 and RANKL and a significant decrease of Notch 1 and Jagged 1 expression were observed compared to control group (P = 0.023, P = 0.005, P = 0.030, and P = 0.001 P = 0.031 and P = 0.029, respectively). Notch 2 and RANKL were also overexpressed in CP group compared to controls (P = 0.001 and P = 0.011). Significant correlations were observed in AP group between expression levels of the analyzed genes. Conclusion The present findings implicate Notch 2 overexpression in the ethiopathogenesis of bone resorption in aggressive and chronic periodontitis. The down-regulation of Notch 1 and Jagged 1 and loss of their osteoprotective function might cause a more excessive osteoclast formation and contribute to greater osteolysis in aggressive periodontitis.",
publisher = "Wiley, Hoboken",
journal = "Journal of Periodontology",
title = "The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis",
volume = "91",
number = "4",
pages = "554-561",
doi = "10.1002/JPER.18-0755"
}

Mijailović, I., Nikolić, N., Đinić, A., Čarkić, J., Milinković, I., Perić, M., Janković, S., Milašin, J.,& Aleksić, Z.. (2020). The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis. in Journal of Periodontology
Wiley, Hoboken., 91(4), 554-561.
https://doi.org/10.1002/JPER.18-0755

Mijailović I, Nikolić N, Đinić A, Čarkić J, Milinković I, Perić M, Janković S, Milašin J, Aleksić Z. The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis. in Journal of Periodontology. 2020;91(4):554-561.
doi:10.1002/JPER.18-0755 .

Mijailović, Iva, Nikolić, Nadja, Đinić, Ana, Čarkić, Jelena, Milinković, Iva, Perić, Mina, Janković, Saša, Milašin, Jelena, Aleksić, Zoran, "The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis" in Journal of Periodontology, 91, no. 4 (2020):554-561,
https://doi.org/10.1002/JPER.18-0755 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Nikolić, Nadja
AU  - Čarkić, Jelena
AU  - Beljić-Ivanović, Katarina
AU  - Soldatović, Ivan
AU  - Miletić, Maja
AU  - Andrić, Miroslav
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1047
AB  - Aim To investigate the possible association between TNF alpha (-308 G/A) and IL-1 beta (-511 C/T) single nucleotide polymorphisms (SNPs) and GSTT and GSTM deletion polymorphisms and risk of apical periodontitis (AP) development, and determine the association of different genotypes with the presence of herpesviral infection in AP. Methodology The study included 120 periapical lesions and 200 control samples. Gene polymorphism analysis was performed using either polymerase chain reaction (PCR) or PCR/ restriction fragment length polymorphism (RFLP). Relative gene expression of TNF-alpha and IL-1 beta was analysed using reverse transcriptase - real-time PCR. The presence of Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) was assessed by nested PCR. Chi-square and Fisher's exact tests and logistic regression analyses were done for polymorphisms, whilst Mann-Whitney U-test was performed for the expression analysis. The expected frequency of variants was analysed by the Hardy-Weinberg equilibrium test. Results TNF-alpha (-308 G/A) SNP increased AP susceptibility for heterozygous (odds ratio (OR) = 1.72, 95% confidence interval (CI) = 1.06-2.80, P = 0.027) and homozygous (OR = 8.55, 95% CI = 1.77-41.36, P  lt  0.001) carriers of the variant A allele. On the other hand, IL-1 beta (-511 C/T) polymorphism exerted a protective effect both in heterozygotes (OR = 0.540, 95% CI = 0.332-0.880, P = 0.013) and homozygotes (OR = 0.114, 95% CI = 0.026-0.501, P  lt  0.001). In addition, GSTM1 and GSTT1 null genotypes separately, as well as concomitantly, were associated with an increased risk for AP development (P  lt  0.001). The null GSTT1 genotype increased approximately twice the risk of Epstein-Barr infection (EBV) in AP (OR = 2.17, 95% CI = 1-4.71, P = 0.048), whilst TNF-alpha SNP decreased it, both in heterozygotes (OR = 0.20, 95% CI = 0.08-0.48, P  lt  0.001) and AA homozygotes (OR = 0.07, 95% CI = 0.01-0.37, P = 0.001). Conclusions GSTM and GSTT deletion polymorphisms, as well as TNF alpha (-308 G/A) SNP, are associated with increased risk, whereas IL-1 beta (-511 C/T) polymorphism decreases the risk of AP development. GSTT and TNF alpha polymorphisms also appear to modulate the risk of EBV infection in Serbian patients with AP.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Association of polymorphisms in TNF-alpha, IL-1 beta, GSTM and GSTT genes with apical periodontitis: is there a link with herpesviral infection?
VL  - 53
IS  - 7
SP  - 895
EP  - 904
DO  - 10.1111/iej.13298
ER  - 

@article{
author = "Jakovljević, Aleksandar and Nikolić, Nadja and Čarkić, Jelena and Beljić-Ivanović, Katarina and Soldatović, Ivan and Miletić, Maja and Andrić, Miroslav and Milašin, Jelena",
year = "2020",
abstract = "Aim To investigate the possible association between TNF alpha (-308 G/A) and IL-1 beta (-511 C/T) single nucleotide polymorphisms (SNPs) and GSTT and GSTM deletion polymorphisms and risk of apical periodontitis (AP) development, and determine the association of different genotypes with the presence of herpesviral infection in AP. Methodology The study included 120 periapical lesions and 200 control samples. Gene polymorphism analysis was performed using either polymerase chain reaction (PCR) or PCR/ restriction fragment length polymorphism (RFLP). Relative gene expression of TNF-alpha and IL-1 beta was analysed using reverse transcriptase - real-time PCR. The presence of Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) was assessed by nested PCR. Chi-square and Fisher's exact tests and logistic regression analyses were done for polymorphisms, whilst Mann-Whitney U-test was performed for the expression analysis. The expected frequency of variants was analysed by the Hardy-Weinberg equilibrium test. Results TNF-alpha (-308 G/A) SNP increased AP susceptibility for heterozygous (odds ratio (OR) = 1.72, 95% confidence interval (CI) = 1.06-2.80, P = 0.027) and homozygous (OR = 8.55, 95% CI = 1.77-41.36, P  lt  0.001) carriers of the variant A allele. On the other hand, IL-1 beta (-511 C/T) polymorphism exerted a protective effect both in heterozygotes (OR = 0.540, 95% CI = 0.332-0.880, P = 0.013) and homozygotes (OR = 0.114, 95% CI = 0.026-0.501, P  lt  0.001). In addition, GSTM1 and GSTT1 null genotypes separately, as well as concomitantly, were associated with an increased risk for AP development (P  lt  0.001). The null GSTT1 genotype increased approximately twice the risk of Epstein-Barr infection (EBV) in AP (OR = 2.17, 95% CI = 1-4.71, P = 0.048), whilst TNF-alpha SNP decreased it, both in heterozygotes (OR = 0.20, 95% CI = 0.08-0.48, P  lt  0.001) and AA homozygotes (OR = 0.07, 95% CI = 0.01-0.37, P = 0.001). Conclusions GSTM and GSTT deletion polymorphisms, as well as TNF alpha (-308 G/A) SNP, are associated with increased risk, whereas IL-1 beta (-511 C/T) polymorphism decreases the risk of AP development. GSTT and TNF alpha polymorphisms also appear to modulate the risk of EBV infection in Serbian patients with AP.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Association of polymorphisms in TNF-alpha, IL-1 beta, GSTM and GSTT genes with apical periodontitis: is there a link with herpesviral infection?",
volume = "53",
number = "7",
pages = "895-904",
doi = "10.1111/iej.13298"
}

Jakovljević, A., Nikolić, N., Čarkić, J., Beljić-Ivanović, K., Soldatović, I., Miletić, M., Andrić, M.,& Milašin, J.. (2020). Association of polymorphisms in TNF-alpha, IL-1 beta, GSTM and GSTT genes with apical periodontitis: is there a link with herpesviral infection?. in International Endodontic Journal
Wiley, Hoboken., 53(7), 895-904.
https://doi.org/10.1111/iej.13298

Jakovljević A, Nikolić N, Čarkić J, Beljić-Ivanović K, Soldatović I, Miletić M, Andrić M, Milašin J. Association of polymorphisms in TNF-alpha, IL-1 beta, GSTM and GSTT genes with apical periodontitis: is there a link with herpesviral infection?. in International Endodontic Journal. 2020;53(7):895-904.
doi:10.1111/iej.13298 .

Jakovljević, Aleksandar, Nikolić, Nadja, Čarkić, Jelena, Beljić-Ivanović, Katarina, Soldatović, Ivan, Miletić, Maja, Andrić, Miroslav, Milašin, Jelena, "Association of polymorphisms in TNF-alpha, IL-1 beta, GSTM and GSTT genes with apical periodontitis: is there a link with herpesviral infection?" in International Endodontic Journal, 53, no. 7 (2020):895-904,
https://doi.org/10.1111/iej.13298 . .

TY  - JOUR
AU  - Andjus, Stefan
AU  - Lazović, Vladimir
AU  - Nikolić, Nadja
AU  - Tubić, Bojana
AU  - Nikolić, Vera
AU  - Paunović, Momir
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2502
AB  - As data on the distribution of freshwater sponges (Porifera, Demospongiae, Spongillida) in Serbia are extremely scarce, we investigated the main Serbian rivers and lakes with respect to Porifera occurrence, for which 17 lotic and 11 lentic water bodies were selected. Sponges were found in 11 of 17 rivers (62 specimens in total) and in 3 of 11 lakes/reservoirs (seven specimens in total). Classical morphological spicule analysis was coupled with polymerase chain reaction (PCR) and gene sequencing for species identification. Among the 69 collected specimens, five sponge species of the family Spongillidae have been identified: Ephydana fluviatilis (Linnaeus, 1759), Spongilla lacustris (Linnaeus, 1759), Ephydatia muelleri (Lieberklihn, 1856), Trochospongilla horrida Weltner, 1893, and Eunapius fragilis (Leidy, 1851). The most frequently found sponge in Serbian rivers was E.fluviatilis (45 % of all specimens), while the least frequent was E. fragilis (6% of all specimens). The Tisa river has the highest sponge diversity (four species). In lentic water bodies, only E.fluviatilis (four specimens) and S. lacustris (three specimens) were found. In general, sponges were infrequent and their abundance was low in Serbian fresh waters. While sponges seem to tolerate significant variations of physical and chemical parameters, some optimal values can be established.
PB  - E Schweizerbartsche Verlagsbuchhandlung, Stuttgart
T2  - Fundamental & Applied Limnology
T1  - Distribution of freshwater sponges in Serbia
VL  - 193
IS  - 3
SP  - 195
EP  - 204
DO  - 10.1127/fal/2019/1242
ER  - 

@article{
author = "Andjus, Stefan and Lazović, Vladimir and Nikolić, Nadja and Tubić, Bojana and Nikolić, Vera and Paunović, Momir",
year = "2020",
abstract = "As data on the distribution of freshwater sponges (Porifera, Demospongiae, Spongillida) in Serbia are extremely scarce, we investigated the main Serbian rivers and lakes with respect to Porifera occurrence, for which 17 lotic and 11 lentic water bodies were selected. Sponges were found in 11 of 17 rivers (62 specimens in total) and in 3 of 11 lakes/reservoirs (seven specimens in total). Classical morphological spicule analysis was coupled with polymerase chain reaction (PCR) and gene sequencing for species identification. Among the 69 collected specimens, five sponge species of the family Spongillidae have been identified: Ephydana fluviatilis (Linnaeus, 1759), Spongilla lacustris (Linnaeus, 1759), Ephydatia muelleri (Lieberklihn, 1856), Trochospongilla horrida Weltner, 1893, and Eunapius fragilis (Leidy, 1851). The most frequently found sponge in Serbian rivers was E.fluviatilis (45 % of all specimens), while the least frequent was E. fragilis (6% of all specimens). The Tisa river has the highest sponge diversity (four species). In lentic water bodies, only E.fluviatilis (four specimens) and S. lacustris (three specimens) were found. In general, sponges were infrequent and their abundance was low in Serbian fresh waters. While sponges seem to tolerate significant variations of physical and chemical parameters, some optimal values can be established.",
publisher = "E Schweizerbartsche Verlagsbuchhandlung, Stuttgart",
journal = "Fundamental & Applied Limnology",
title = "Distribution of freshwater sponges in Serbia",
volume = "193",
number = "3",
pages = "195-204",
doi = "10.1127/fal/2019/1242"
}

Andjus, S., Lazović, V., Nikolić, N., Tubić, B., Nikolić, V.,& Paunović, M.. (2020). Distribution of freshwater sponges in Serbia. in Fundamental & Applied Limnology
E Schweizerbartsche Verlagsbuchhandlung, Stuttgart., 193(3), 195-204.
https://doi.org/10.1127/fal/2019/1242

Andjus S, Lazović V, Nikolić N, Tubić B, Nikolić V, Paunović M. Distribution of freshwater sponges in Serbia. in Fundamental & Applied Limnology. 2020;193(3):195-204.
doi:10.1127/fal/2019/1242 .

Andjus, Stefan, Lazović, Vladimir, Nikolić, Nadja, Tubić, Bojana, Nikolić, Vera, Paunović, Momir, "Distribution of freshwater sponges in Serbia" in Fundamental & Applied Limnology, 193, no. 3 (2020):195-204,
https://doi.org/10.1127/fal/2019/1242 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Miletić, Maja
AU  - Nikolić, Nadja
AU  - Beljić-Ivanović, Katarina
AU  - Andrić, Miroslav
AU  - Milašin, Jelena
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2455
AB  - Apical periodontitis represents a chronic inflammatory process within periapical tissues, mostly caused by etiological agents of endodontic origin. Progressive bone resorption in the periapical region represents the hallmark of apical periodontitis and occurs as the consequence of interplay between polymicrobial infections and host response. The Notch signaling pathway is an evolutionary conserved cell-signaling system that plays an important role in a variety of cell functions including proliferation, differentiation and apoptosis. In recent years its involvement in bone homeostasis has attracted a significant consideration. We hypothesized that Notch signaling pathway, which has a complex interplay with proinflammatory cytokines and bone resorption regulators, contributes to alveolar bone resorption via increased Notch receptors on immune cell surface and stimulates Notch receptor intracellular domain (NICD) translocation into the nucleus. The potential benefit of medications aimed to down-regulate these pathways in apical periodontitis treatment remains to be assessed.
PB  - Churchill Livingstone, Edinburgh
T2  - Medical Hypotheses
T1  - Notch signaling pathway mediates alveolar bone resorption in apical periodontitis
VL  - 124
SP  - 87
EP  - 90
DO  - 10.1016/j.mehy.2019.02.018
ER  - 

@article{
author = "Jakovljević, Aleksandar and Miletić, Maja and Nikolić, Nadja and Beljić-Ivanović, Katarina and Andrić, Miroslav and Milašin, Jelena",
year = "2019",
abstract = "Apical periodontitis represents a chronic inflammatory process within periapical tissues, mostly caused by etiological agents of endodontic origin. Progressive bone resorption in the periapical region represents the hallmark of apical periodontitis and occurs as the consequence of interplay between polymicrobial infections and host response. The Notch signaling pathway is an evolutionary conserved cell-signaling system that plays an important role in a variety of cell functions including proliferation, differentiation and apoptosis. In recent years its involvement in bone homeostasis has attracted a significant consideration. We hypothesized that Notch signaling pathway, which has a complex interplay with proinflammatory cytokines and bone resorption regulators, contributes to alveolar bone resorption via increased Notch receptors on immune cell surface and stimulates Notch receptor intracellular domain (NICD) translocation into the nucleus. The potential benefit of medications aimed to down-regulate these pathways in apical periodontitis treatment remains to be assessed.",
publisher = "Churchill Livingstone, Edinburgh",
journal = "Medical Hypotheses",
title = "Notch signaling pathway mediates alveolar bone resorption in apical periodontitis",
volume = "124",
pages = "87-90",
doi = "10.1016/j.mehy.2019.02.018"
}

Jakovljević, A., Miletić, M., Nikolić, N., Beljić-Ivanović, K., Andrić, M.,& Milašin, J.. (2019). Notch signaling pathway mediates alveolar bone resorption in apical periodontitis. in Medical Hypotheses
Churchill Livingstone, Edinburgh., 124, 87-90.
https://doi.org/10.1016/j.mehy.2019.02.018

Jakovljević A, Miletić M, Nikolić N, Beljić-Ivanović K, Andrić M, Milašin J. Notch signaling pathway mediates alveolar bone resorption in apical periodontitis. in Medical Hypotheses. 2019;124:87-90.
doi:10.1016/j.mehy.2019.02.018 .

Jakovljević, Aleksandar, Miletić, Maja, Nikolić, Nadja, Beljić-Ivanović, Katarina, Andrić, Miroslav, Milašin, Jelena, "Notch signaling pathway mediates alveolar bone resorption in apical periodontitis" in Medical Hypotheses, 124 (2019):87-90,
https://doi.org/10.1016/j.mehy.2019.02.018 . .

TY  - JOUR
AU  - Šljivančanin Jakovljević, Tamara
AU  - Kontić-Vučinić, Olivera
AU  - Nikolić, Nadja
AU  - Čarkić, Jelena
AU  - Soldatović, Ivan
AU  - Milašin, Jelena
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2421
AB  - Problem Preeclampsia has a multifactorial origin with genetic, immunological, and environmental factors described as main contributors to its onset. This study aimed to investigate glutathione-S-transferase M1 (GSTM1) and glutathione-S-transferase T1 (GSTT1) gene polymorphisms, the expression of pro-inflammatory cytokines (TNF-alpha, IL-1 beta, IL-6), and the potential relationship between GST polymorphisms and cytokine expression levels in preeclampsia and uncomplicated pregnancy. Method of Study This prospective case-control study included 50 women with preeclampsia and 50 healthy pregnant women. DNA and RNA were extracted from women leukocytes. Deletion polymorphisms were analyzed by PCR, while cytokine mRNA expression was analyzed by real-time PCR. Results GSTM1 null genotype with present GSTT1 increased the risk for preeclampsia development. Deletion of GSTT1 without deletion of GSTM1 increased the risk for early preeclampsia. Relative mRNA expression of TNF-alpha was significantly higher in preeclampsia compared to healthy pregnant women (P = 0.006). Expression of IL-1 beta was significantly higher in severe and late preeclampsia compared to the control group (P = 0.005, P = 0.007, respectively). A significant positive correlation between TNF-alpha and IL-1 beta was observed (Spearman's rho = 0.312, P = 0.028) and between IL-1 beta and IL-6, in preeclampsia group (Spearman's rho = 0.296, P = 0.037). IL-1 beta was significantly increased in patients with GSTT1 null genotype (P = 0.015) while IL-6 was increased in patients with GSTM1 null genotype (P = 0.015). Conclusions GSTM1 null genotype represents a risk factor for preeclampsia development, while GSTT1 null genotype favors early preeclampsia. Preeclampsia is also associated with increased expression of pro-inflammatory cytokines, predominantly TNF-alpha and IL-1 beta.
PB  - Wiley, Hoboken
T2  - American Journal of Reproductive Immunology
T1  - Glutathione-S-transferase M1 polymorphism and pro-inflammatory cytokines tumour necrosis factor-alpha and interleukin-1 beta are associated with preeclampsia in Serbian women
VL  - 81
IS  - 5
DO  - 10.1111/aji.13105
ER  - 

@article{
author = "Šljivančanin Jakovljević, Tamara and Kontić-Vučinić, Olivera and Nikolić, Nadja and Čarkić, Jelena and Soldatović, Ivan and Milašin, Jelena",
year = "2019",
abstract = "Problem Preeclampsia has a multifactorial origin with genetic, immunological, and environmental factors described as main contributors to its onset. This study aimed to investigate glutathione-S-transferase M1 (GSTM1) and glutathione-S-transferase T1 (GSTT1) gene polymorphisms, the expression of pro-inflammatory cytokines (TNF-alpha, IL-1 beta, IL-6), and the potential relationship between GST polymorphisms and cytokine expression levels in preeclampsia and uncomplicated pregnancy. Method of Study This prospective case-control study included 50 women with preeclampsia and 50 healthy pregnant women. DNA and RNA were extracted from women leukocytes. Deletion polymorphisms were analyzed by PCR, while cytokine mRNA expression was analyzed by real-time PCR. Results GSTM1 null genotype with present GSTT1 increased the risk for preeclampsia development. Deletion of GSTT1 without deletion of GSTM1 increased the risk for early preeclampsia. Relative mRNA expression of TNF-alpha was significantly higher in preeclampsia compared to healthy pregnant women (P = 0.006). Expression of IL-1 beta was significantly higher in severe and late preeclampsia compared to the control group (P = 0.005, P = 0.007, respectively). A significant positive correlation between TNF-alpha and IL-1 beta was observed (Spearman's rho = 0.312, P = 0.028) and between IL-1 beta and IL-6, in preeclampsia group (Spearman's rho = 0.296, P = 0.037). IL-1 beta was significantly increased in patients with GSTT1 null genotype (P = 0.015) while IL-6 was increased in patients with GSTM1 null genotype (P = 0.015). Conclusions GSTM1 null genotype represents a risk factor for preeclampsia development, while GSTT1 null genotype favors early preeclampsia. Preeclampsia is also associated with increased expression of pro-inflammatory cytokines, predominantly TNF-alpha and IL-1 beta.",
publisher = "Wiley, Hoboken",
journal = "American Journal of Reproductive Immunology",
title = "Glutathione-S-transferase M1 polymorphism and pro-inflammatory cytokines tumour necrosis factor-alpha and interleukin-1 beta are associated with preeclampsia in Serbian women",
volume = "81",
number = "5",
doi = "10.1111/aji.13105"
}

Šljivančanin Jakovljević, T., Kontić-Vučinić, O., Nikolić, N., Čarkić, J., Soldatović, I.,& Milašin, J.. (2019). Glutathione-S-transferase M1 polymorphism and pro-inflammatory cytokines tumour necrosis factor-alpha and interleukin-1 beta are associated with preeclampsia in Serbian women. in American Journal of Reproductive Immunology
Wiley, Hoboken., 81(5).
https://doi.org/10.1111/aji.13105

Šljivančanin Jakovljević T, Kontić-Vučinić O, Nikolić N, Čarkić J, Soldatović I, Milašin J. Glutathione-S-transferase M1 polymorphism and pro-inflammatory cytokines tumour necrosis factor-alpha and interleukin-1 beta are associated with preeclampsia in Serbian women. in American Journal of Reproductive Immunology. 2019;81(5).
doi:10.1111/aji.13105 .

Šljivančanin Jakovljević, Tamara, Kontić-Vučinić, Olivera, Nikolić, Nadja, Čarkić, Jelena, Soldatović, Ivan, Milašin, Jelena, "Glutathione-S-transferase M1 polymorphism and pro-inflammatory cytokines tumour necrosis factor-alpha and interleukin-1 beta are associated with preeclampsia in Serbian women" in American Journal of Reproductive Immunology, 81, no. 5 (2019),
https://doi.org/10.1111/aji.13105 . .

TY  - JOUR
AU  - Simonović, Jelena
AU  - Toljić, Boško
AU  - Rasković, Bozidar
AU  - Jovanović, Vladimir
AU  - Lazarević, Miloš
AU  - Milošević, Maja
AU  - Nikolić, Nadja
AU  - Panajotović, Radmila
AU  - Milašin, Jelena
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2397
AB  - Aim To characterize stem cells originating from different dental tissues (apical papilla [SCAP], dental follicle [DFSC], and pulp [DPSC]) and test the capacity of Raman microspectroscopy to distinguish between the three dental stem cell types. Methods SCAR DFSC, and DPSC cultures were generated from three immature wisdom teeth originating from three patients. Cell stemness was confirmed by inducing neuro-, osteo-, chondro-, and adipo-differentiaton and by mesenchymal marker expression analysis by flow-cytometry and real-time polymerase chain reaction. Cellular components were then evaluated by Raman microspectroscopy. Results We found differences between SCAP, DFSC, and DPSC Raman spectra. The ratio between proteins and nucleic acids (748/770), a parameter for discriminating more differentiated from less differentiated cells, showed significant differences between the three cell types. All cells also displayed a fingerprint region in the 600-700 cm(-1) range, and characteristic lipid peaks at positions 1440 cm(-1) and 1650 cm(-1). Conclusion Although different dental stem cells exhibited similar Raman spectra, the method enabled us to make subtle distinction between them.
PB  - Medicinska Naklada, Zagreb
T2  - Croatian Medical Journal
T1  - Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells
VL  - 60
IS  - 2
SP  - 78
EP  - 86
DO  - 10.3325/CroatMedJ_60_0078
ER  - 

@article{
author = "Simonović, Jelena and Toljić, Boško and Rasković, Bozidar and Jovanović, Vladimir and Lazarević, Miloš and Milošević, Maja and Nikolić, Nadja and Panajotović, Radmila and Milašin, Jelena",
year = "2019",
abstract = "Aim To characterize stem cells originating from different dental tissues (apical papilla [SCAP], dental follicle [DFSC], and pulp [DPSC]) and test the capacity of Raman microspectroscopy to distinguish between the three dental stem cell types. Methods SCAR DFSC, and DPSC cultures were generated from three immature wisdom teeth originating from three patients. Cell stemness was confirmed by inducing neuro-, osteo-, chondro-, and adipo-differentiaton and by mesenchymal marker expression analysis by flow-cytometry and real-time polymerase chain reaction. Cellular components were then evaluated by Raman microspectroscopy. Results We found differences between SCAP, DFSC, and DPSC Raman spectra. The ratio between proteins and nucleic acids (748/770), a parameter for discriminating more differentiated from less differentiated cells, showed significant differences between the three cell types. All cells also displayed a fingerprint region in the 600-700 cm(-1) range, and characteristic lipid peaks at positions 1440 cm(-1) and 1650 cm(-1). Conclusion Although different dental stem cells exhibited similar Raman spectra, the method enabled us to make subtle distinction between them.",
publisher = "Medicinska Naklada, Zagreb",
journal = "Croatian Medical Journal",
title = "Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells",
volume = "60",
number = "2",
pages = "78-86",
doi = "10.3325/CroatMedJ_60_0078"
}

Simonović, J., Toljić, B., Rasković, B., Jovanović, V., Lazarević, M., Milošević, M., Nikolić, N., Panajotović, R.,& Milašin, J.. (2019). Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells. in Croatian Medical Journal
Medicinska Naklada, Zagreb., 60(2), 78-86.
https://doi.org/10.3325/CroatMedJ_60_0078

Simonović J, Toljić B, Rasković B, Jovanović V, Lazarević M, Milošević M, Nikolić N, Panajotović R, Milašin J. Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells. in Croatian Medical Journal. 2019;60(2):78-86.
doi:10.3325/CroatMedJ_60_0078 .

Simonović, Jelena, Toljić, Boško, Rasković, Bozidar, Jovanović, Vladimir, Lazarević, Miloš, Milošević, Maja, Nikolić, Nadja, Panajotović, Radmila, Milašin, Jelena, "Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells" in Croatian Medical Journal, 60, no. 2 (2019):78-86,
https://doi.org/10.3325/CroatMedJ_60_0078 . .

TY  - JOUR
AU  - Nikolić, Nadja
AU  - Jakovljević, Aleksandar
AU  - Čarkić, Jelena
AU  - Beljić-Ivanović, Katarina
AU  - Miletić, Maja
AU  - Soldatović, Ivan
AU  - Andrić, Miroslav
AU  - Ivanović, Vladimir
AU  - Milašin, Jelena
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2390
AB  - Introduction: The exact mechanisms of periapical bone resorption have not been fully elucidated. This study aimed to analyze the expression of Notch signaling molecules (Notch2, Jagged1, and Hey1) and proinflammatory cytokines (tumor necrosis factor alpha [TNF-alpha], interleukin [IL]-1 beta, and IL-6) in human apical periodontitis lesions with different receptor activator of nuclear factor kappa B ligand (RANKL)/osteo-protegerin (OPG) ratios and determine their potential correlation. Methods: The study group consisted of 50 periapical lesions collected in conjunction with apicoectomy. The relative gene expression of the investigated molecules (Notch2, Jagged1, Hey1, RANKL, OPG, TNF-alpha, IL-1 beta, and IL-6) in all tissue samples was analyzed using reverse transcriptase real-time polymerase chain reaction. The Student t test, Mann-Whitney U test and Spearman correlation were used for statistical analysis. Results: Based on the RANKUOPG ratio, periapical lesions were either RANKL predominant (RANKL > OPG, n = 33) or OPG predominant (RANKL  lt  OPG, n = 17). Symptomatic lesions occurred more frequently in RANKL-predominant compared with OPG-predominant lesions (24 vs 7, P=.029). Notch2,Jagged1, Hey1, and TNF-alpha were significantly overexpressed in lesions with predominant RANKL compared with lesions with predominant OPG (P =.001, P =.001, P =.027, and P =.016, respectively). Significant correlations were observed between the investigated genes in periapical lesions. Conclusions: Notch signaling appeared to be activated in periapical inflammation. An increase in Notch2, Jagged1, Hey1, and TNF-alpha expression in RANKL-predominant periapical lesions corroborates their joined involvement in extensive periapical bone resorption.
PB  - Elsevier Science Inc, New York
T2  - Journal of Endodontics
T1  - Notch Signaling Pathway in Apical Periodontitis: Correlation with Bone Resorption Regulators and Proinflammatory Cytokines
VL  - 45
IS  - 2
SP  - 123
EP  - 128
DO  - 10.1016/j.joen.2018.10.015
ER  - 

@article{
author = "Nikolić, Nadja and Jakovljević, Aleksandar and Čarkić, Jelena and Beljić-Ivanović, Katarina and Miletić, Maja and Soldatović, Ivan and Andrić, Miroslav and Ivanović, Vladimir and Milašin, Jelena",
year = "2019",
abstract = "Introduction: The exact mechanisms of periapical bone resorption have not been fully elucidated. This study aimed to analyze the expression of Notch signaling molecules (Notch2, Jagged1, and Hey1) and proinflammatory cytokines (tumor necrosis factor alpha [TNF-alpha], interleukin [IL]-1 beta, and IL-6) in human apical periodontitis lesions with different receptor activator of nuclear factor kappa B ligand (RANKL)/osteo-protegerin (OPG) ratios and determine their potential correlation. Methods: The study group consisted of 50 periapical lesions collected in conjunction with apicoectomy. The relative gene expression of the investigated molecules (Notch2, Jagged1, Hey1, RANKL, OPG, TNF-alpha, IL-1 beta, and IL-6) in all tissue samples was analyzed using reverse transcriptase real-time polymerase chain reaction. The Student t test, Mann-Whitney U test and Spearman correlation were used for statistical analysis. Results: Based on the RANKUOPG ratio, periapical lesions were either RANKL predominant (RANKL > OPG, n = 33) or OPG predominant (RANKL  lt  OPG, n = 17). Symptomatic lesions occurred more frequently in RANKL-predominant compared with OPG-predominant lesions (24 vs 7, P=.029). Notch2,Jagged1, Hey1, and TNF-alpha were significantly overexpressed in lesions with predominant RANKL compared with lesions with predominant OPG (P =.001, P =.001, P =.027, and P =.016, respectively). Significant correlations were observed between the investigated genes in periapical lesions. Conclusions: Notch signaling appeared to be activated in periapical inflammation. An increase in Notch2, Jagged1, Hey1, and TNF-alpha expression in RANKL-predominant periapical lesions corroborates their joined involvement in extensive periapical bone resorption.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Endodontics",
title = "Notch Signaling Pathway in Apical Periodontitis: Correlation with Bone Resorption Regulators and Proinflammatory Cytokines",
volume = "45",
number = "2",
pages = "123-128",
doi = "10.1016/j.joen.2018.10.015"
}

Nikolić, N., Jakovljević, A., Čarkić, J., Beljić-Ivanović, K., Miletić, M., Soldatović, I., Andrić, M., Ivanović, V.,& Milašin, J.. (2019). Notch Signaling Pathway in Apical Periodontitis: Correlation with Bone Resorption Regulators and Proinflammatory Cytokines. in Journal of Endodontics
Elsevier Science Inc, New York., 45(2), 123-128.
https://doi.org/10.1016/j.joen.2018.10.015

Nikolić N, Jakovljević A, Čarkić J, Beljić-Ivanović K, Miletić M, Soldatović I, Andrić M, Ivanović V, Milašin J. Notch Signaling Pathway in Apical Periodontitis: Correlation with Bone Resorption Regulators and Proinflammatory Cytokines. in Journal of Endodontics. 2019;45(2):123-128.
doi:10.1016/j.joen.2018.10.015 .

Nikolić, Nadja, Jakovljević, Aleksandar, Čarkić, Jelena, Beljić-Ivanović, Katarina, Miletić, Maja, Soldatović, Ivan, Andrić, Miroslav, Ivanović, Vladimir, Milašin, Jelena, "Notch Signaling Pathway in Apical Periodontitis: Correlation with Bone Resorption Regulators and Proinflammatory Cytokines" in Journal of Endodontics, 45, no. 2 (2019):123-128,
https://doi.org/10.1016/j.joen.2018.10.015 . .

TY  - JOUR
AU  - Andjus, Stefan
AU  - Nikolić, Nadja
AU  - Marjanović, Ana
AU  - Branković, Marija
AU  - Lazović, Vladimir
AU  - Tubić, Bojana
AU  - Čanak-Atlagić, Jelena
AU  - Nikolić, Vera
AU  - Paunović, Momir
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2381
AB  - Trochospongilla horrida Weltner, 1893 (Spongillidae, Spongillida) has a relatively wide, though discontinuous, distribution in the Northern Hemisphere. The aim of this paper was to report the first finding of T. horrida in Serbia. During 2017, research was carried out on ten tributaries of the Sava and the Danube. T. horrida was identified at three localities in Tisa, Juzna Morava and Zapadna Morava rivers, out of 51 inspected sites. For the first time ribosomal 28S DNA was used for T. horrida identification.
PB  - Elsevier Gmbh, Munich
T2  - Limnologica
T1  - First record of freshwater sponge Trochospongilla horrida Weltner, 1893 in Serbia - A morphological and genetic study
VL  - 76
SP  - 48
EP  - 51
DO  - 10.1016/j.limno.2019.03.004
ER  - 

@article{
author = "Andjus, Stefan and Nikolić, Nadja and Marjanović, Ana and Branković, Marija and Lazović, Vladimir and Tubić, Bojana and Čanak-Atlagić, Jelena and Nikolić, Vera and Paunović, Momir",
year = "2019",
abstract = "Trochospongilla horrida Weltner, 1893 (Spongillidae, Spongillida) has a relatively wide, though discontinuous, distribution in the Northern Hemisphere. The aim of this paper was to report the first finding of T. horrida in Serbia. During 2017, research was carried out on ten tributaries of the Sava and the Danube. T. horrida was identified at three localities in Tisa, Juzna Morava and Zapadna Morava rivers, out of 51 inspected sites. For the first time ribosomal 28S DNA was used for T. horrida identification.",
publisher = "Elsevier Gmbh, Munich",
journal = "Limnologica",
title = "First record of freshwater sponge Trochospongilla horrida Weltner, 1893 in Serbia - A morphological and genetic study",
volume = "76",
pages = "48-51",
doi = "10.1016/j.limno.2019.03.004"
}

Andjus, S., Nikolić, N., Marjanović, A., Branković, M., Lazović, V., Tubić, B., Čanak-Atlagić, J., Nikolić, V.,& Paunović, M.. (2019). First record of freshwater sponge Trochospongilla horrida Weltner, 1893 in Serbia - A morphological and genetic study. in Limnologica
Elsevier Gmbh, Munich., 76, 48-51.
https://doi.org/10.1016/j.limno.2019.03.004

Andjus S, Nikolić N, Marjanović A, Branković M, Lazović V, Tubić B, Čanak-Atlagić J, Nikolić V, Paunović M. First record of freshwater sponge Trochospongilla horrida Weltner, 1893 in Serbia - A morphological and genetic study. in Limnologica. 2019;76:48-51.
doi:10.1016/j.limno.2019.03.004 .

Andjus, Stefan, Nikolić, Nadja, Marjanović, Ana, Branković, Marija, Lazović, Vladimir, Tubić, Bojana, Čanak-Atlagić, Jelena, Nikolić, Vera, Paunović, Momir, "First record of freshwater sponge Trochospongilla horrida Weltner, 1893 in Serbia - A morphological and genetic study" in Limnologica, 76 (2019):48-51,
https://doi.org/10.1016/j.limno.2019.03.004 . .

TY  - JOUR
AU  - Eljabo, Najib
AU  - Nikolić, Nadja
AU  - Čarkić, Jelena
AU  - Jelovac, Drago
AU  - Lazarević, M.
AU  - Tanić, Nasta
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2301
AB  - Despite adequate surgical resection, oral squamous cell carcinoma (OSCC) shows a high rate of recurrence and metastasis, which could be explained by the presence of molecular alterations in seemingly normal tumour margins and the entire oral mucosa. The aims of this study were (1) to assess the presence of gene amplification (c-Myc and HER2) and promoter methylation (p14 and p16) in the tumours, tumour margins, and unaffected oral mucosa of 40 OSCC patients, and (2) to evaluate the possibility of using these alterations as prognostic markers. c-Myc and HER2 genes were quantified by means of real-time PCR (qPCR), and p14 and p16 methylation status was determined by methylation-specific PCR (MSP PCR). All tissues examined exhibited molecular alterations in various proportions. Tumour tissues, as expected, showed the highest prevalence of alterations, while oral mucosa showed the lowest. Multiple alterations (co-alterations) in tumours and tumour margins were significantly more frequent than in unaffected oral mucosa (P  lt  0.001 and P = 0.027, respectively). HER2 amplification in margin tissue (P  lt  0.001) and swabs (P = 0.013), as well as the existence of three co-alterations in margins (P = 0.001) and macroscopically unaffected oral mucosa (P  lt  0.001) were correlated with shorter disease-specific survival.
PB  - Churchill Livingstone, Edinburgh
T2  - International Journal of Oral & Maxillofacial Surgery
T1  - Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer
VL  - 47
IS  - 8
SP  - 976
EP  - 982
DO  - 10.1016/j.ijom.2018.01.020
ER  - 

@article{
author = "Eljabo, Najib and Nikolić, Nadja and Čarkić, Jelena and Jelovac, Drago and Lazarević, M. and Tanić, Nasta and Milašin, Jelena",
year = "2018",
abstract = "Despite adequate surgical resection, oral squamous cell carcinoma (OSCC) shows a high rate of recurrence and metastasis, which could be explained by the presence of molecular alterations in seemingly normal tumour margins and the entire oral mucosa. The aims of this study were (1) to assess the presence of gene amplification (c-Myc and HER2) and promoter methylation (p14 and p16) in the tumours, tumour margins, and unaffected oral mucosa of 40 OSCC patients, and (2) to evaluate the possibility of using these alterations as prognostic markers. c-Myc and HER2 genes were quantified by means of real-time PCR (qPCR), and p14 and p16 methylation status was determined by methylation-specific PCR (MSP PCR). All tissues examined exhibited molecular alterations in various proportions. Tumour tissues, as expected, showed the highest prevalence of alterations, while oral mucosa showed the lowest. Multiple alterations (co-alterations) in tumours and tumour margins were significantly more frequent than in unaffected oral mucosa (P  lt  0.001 and P = 0.027, respectively). HER2 amplification in margin tissue (P  lt  0.001) and swabs (P = 0.013), as well as the existence of three co-alterations in margins (P = 0.001) and macroscopically unaffected oral mucosa (P  lt  0.001) were correlated with shorter disease-specific survival.",
publisher = "Churchill Livingstone, Edinburgh",
journal = "International Journal of Oral & Maxillofacial Surgery",
title = "Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer",
volume = "47",
number = "8",
pages = "976-982",
doi = "10.1016/j.ijom.2018.01.020"
}

Eljabo, N., Nikolić, N., Čarkić, J., Jelovac, D., Lazarević, M., Tanić, N.,& Milašin, J.. (2018). Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer. in International Journal of Oral & Maxillofacial Surgery
Churchill Livingstone, Edinburgh., 47(8), 976-982.
https://doi.org/10.1016/j.ijom.2018.01.020

Eljabo N, Nikolić N, Čarkić J, Jelovac D, Lazarević M, Tanić N, Milašin J. Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer. in International Journal of Oral & Maxillofacial Surgery. 2018;47(8):976-982.
doi:10.1016/j.ijom.2018.01.020 .

Eljabo, Najib, Nikolić, Nadja, Čarkić, Jelena, Jelovac, Drago, Lazarević, M., Tanić, Nasta, Milašin, Jelena, "Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer" in International Journal of Oral & Maxillofacial Surgery, 47, no. 8 (2018):976-982,
https://doi.org/10.1016/j.ijom.2018.01.020 . .

TY  - JOUR
AU  - Lazarević, Miloš
AU  - Milošević, Maja
AU  - Trišić, Dijana
AU  - Toljić, Boško
AU  - Simonović, Jelena
AU  - Nikolić, Nadja
AU  - Miković, Nikola
AU  - Jelovac, Drago
AU  - Petrović, Milan
AU  - Vukadinović, Miroslav
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2271
AB  - Purpose: Recent evidence suggests that small subpopulations of stem-like cells are accountable for tumour initiation, progression and metastasis. Until now, studies were focused exclusively on the characterization of these cell populations within the tumour itself, while tumour margins were neglected, although it is known that the histological and molecular status of tumour margins may play a significant role in the course of the disease. Therefore, the aims of the study were to isolate cells from oral squamous cell carcinomas and their respective margins, to characterize these cells using specific markers, to assess their self-renewal potential and determine their chemoresistance. Methods: Cell cultures were obtained from 12 tissue specimens (6 tumours and 6 margins). Total RNA was extracted and gene expression analysis was done by real-time PCR (RTPCR). Flow cytometry, immunocytometry, sphere formation and MTT assays were also applied. Results: With minor differences, cells originating from both tumours and tumour margins showed the presence of stem cell markers CD133, Nanog, Sox2, CD44, and Oct4, had the capacity to form spheroids and showed chemoresistance. Conclusions: Subpopulations of margin cells appeared to have sternness properties which might raise the question of re-evaluation of optimal surgical management.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma
VL  - 23
IS  - 6
SP  - 1686
EP  - 1692
UR  - https://hdl.handle.net/21.15107/rcub_smile_2271
ER  - 

@article{
author = "Lazarević, Miloš and Milošević, Maja and Trišić, Dijana and Toljić, Boško and Simonović, Jelena and Nikolić, Nadja and Miković, Nikola and Jelovac, Drago and Petrović, Milan and Vukadinović, Miroslav and Milašin, Jelena",
year = "2018",
abstract = "Purpose: Recent evidence suggests that small subpopulations of stem-like cells are accountable for tumour initiation, progression and metastasis. Until now, studies were focused exclusively on the characterization of these cell populations within the tumour itself, while tumour margins were neglected, although it is known that the histological and molecular status of tumour margins may play a significant role in the course of the disease. Therefore, the aims of the study were to isolate cells from oral squamous cell carcinomas and their respective margins, to characterize these cells using specific markers, to assess their self-renewal potential and determine their chemoresistance. Methods: Cell cultures were obtained from 12 tissue specimens (6 tumours and 6 margins). Total RNA was extracted and gene expression analysis was done by real-time PCR (RTPCR). Flow cytometry, immunocytometry, sphere formation and MTT assays were also applied. Results: With minor differences, cells originating from both tumours and tumour margins showed the presence of stem cell markers CD133, Nanog, Sox2, CD44, and Oct4, had the capacity to form spheroids and showed chemoresistance. Conclusions: Subpopulations of margin cells appeared to have sternness properties which might raise the question of re-evaluation of optimal surgical management.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma",
volume = "23",
number = "6",
pages = "1686-1692",
url = "https://hdl.handle.net/21.15107/rcub_smile_2271"
}

Lazarević, M., Milošević, M., Trišić, D., Toljić, B., Simonović, J., Nikolić, N., Miković, N., Jelovac, D., Petrović, M., Vukadinović, M.,& Milašin, J.. (2018). Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 23(6), 1686-1692.
https://hdl.handle.net/21.15107/rcub_smile_2271

Lazarević M, Milošević M, Trišić D, Toljić B, Simonović J, Nikolić N, Miković N, Jelovac D, Petrović M, Vukadinović M, Milašin J. Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma. in Journal of BUON. 2018;23(6):1686-1692.
https://hdl.handle.net/21.15107/rcub_smile_2271 .

Lazarević, Miloš, Milošević, Maja, Trišić, Dijana, Toljić, Boško, Simonović, Jelena, Nikolić, Nadja, Miković, Nikola, Jelovac, Drago, Petrović, Milan, Vukadinović, Miroslav, Milašin, Jelena, "Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma" in Journal of BUON, 23, no. 6 (2018):1686-1692,
https://hdl.handle.net/21.15107/rcub_smile_2271 .

TY  - JOUR
AU  - Dragović, Miroslav
AU  - Pejović, Marko
AU  - Stepić, Jelena
AU  - Dragović, Svetlana
AU  - Nikolić, Nadja
AU  - Kuzmanović-Pfićer, Jovana
AU  - Čolić, Snježana
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2343
AB  - Introduction/Objective The purpose of this study was to compare polypropylene and silk suture materials in terms of bacterial adherence and clinical features including the impact on soft tissue healing. Methods Ten healthy patients were included in this study. Unilateral upper and lower wisdom teeth were extracted at the same time and wounds were sutured with different threads (one monofilament - polypropylene - and one multifilament - silk suture). Stitches were removed seven days postoperatively. Real-time polymerase chain reaction was used to analyze bacterial adherence. Intraoperative handling and ease of removal were assessed with the help of Visual Analogue Scale. Landry healing index was used for evaluation of soft tissue healing. Results Significantly more pronounced bacterial adherence was found on silk compared to polypropylene sutures (p = 0.005). Superior intraoperative handling properties were registered suturing with polypropylene compared to silk (p = 0.005). Soft tissue healing was significantly better around polypropylene sutures, both on the third and the seventh postoperative day (p = 0.016). Patient discomfort was slightly higher for polypropylene sutures, but without statistical significance. Conclusion Polypropylene suture material showed significantly lower bacterial adherence and superior clinical features compared to silk, including better soft tissue healing.
AB  - Uvod/Cilj Cilj ove studije bio je poređenje svilenog (SK) i polipropilenskog konca (PPK) u pogledu prijemčivosti za bakterije i kliničkih karakteristika, uključujući uticaj na zarastanje mekog tkiva u usnoj duplji. Metode U studiju je uključeno deset zdravih ispitanika kod kojih su hirurški izvađeni gornji i donji umnjak sa jedne strane istovremeno, a rane su ušivene različitim koncima (jedan monofilamentni - PPK i jedan polifilamentni - SK). Kvantifikacija bakterija na uzorcima konaca koji su uklonjeni sedam dana posle operacije urađena je metodom lančane reakcije polimeraze u realnom vremenu. Oralni hirurg je uz pomoć Vizuelne analogne skale ocenjivao lakoću intraoperativnog rukovanja, kao i lakoću uklanjanja konaca. Za procenu kvaliteta zarastanja mekog tkiva korišćen je indeks po Landriju. Rezultati Statistički značajno više bakterija nađeno je na svim uzorcima SK u poređenju sa PPK (p = 0,005). PPK se pokazao značajno lakšim za intraoperativno rukovanje u odnosu na SK (p = 0,005). Takođe, zarastanje mekog tkiva, 3. i 7. dana postoperativno, bilo je značajno uspešnije oko PPK nego oko SK (p = 0,016). Neprijatnost zbog prisustva konaca bila je veća kod primene PPK u odnosu na SK, ali bez statistički značajne razlike. Zaključak Polipropilenski konac je u odnosu na svileni konac pokazao značajno manju prijemčivost za bakterije i bolje kliničke karakteristike, uključujući i bolje zarastanje mekog tkiva. PR Projekat Ministarstva nauke Republike Srbije, br. 175075.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Microbial adherence affinity and clinical characteristics of polypropylene versus silk sutures in oral surgery
T1  - Svileni i polipropilenski materijal za šavove u oralnoj hirurgiji - kolonizacija mikroorganizmima i kliničke karakteristike
VL  - 146
IS  - 5-6
SP  - 258
EP  - 263
DO  - 10.2298/SARH170428184D
ER  - 

@article{
author = "Dragović, Miroslav and Pejović, Marko and Stepić, Jelena and Dragović, Svetlana and Nikolić, Nadja and Kuzmanović-Pfićer, Jovana and Čolić, Snježana and Milašin, Jelena",
year = "2018",
abstract = "Introduction/Objective The purpose of this study was to compare polypropylene and silk suture materials in terms of bacterial adherence and clinical features including the impact on soft tissue healing. Methods Ten healthy patients were included in this study. Unilateral upper and lower wisdom teeth were extracted at the same time and wounds were sutured with different threads (one monofilament - polypropylene - and one multifilament - silk suture). Stitches were removed seven days postoperatively. Real-time polymerase chain reaction was used to analyze bacterial adherence. Intraoperative handling and ease of removal were assessed with the help of Visual Analogue Scale. Landry healing index was used for evaluation of soft tissue healing. Results Significantly more pronounced bacterial adherence was found on silk compared to polypropylene sutures (p = 0.005). Superior intraoperative handling properties were registered suturing with polypropylene compared to silk (p = 0.005). Soft tissue healing was significantly better around polypropylene sutures, both on the third and the seventh postoperative day (p = 0.016). Patient discomfort was slightly higher for polypropylene sutures, but without statistical significance. Conclusion Polypropylene suture material showed significantly lower bacterial adherence and superior clinical features compared to silk, including better soft tissue healing., Uvod/Cilj Cilj ove studije bio je poređenje svilenog (SK) i polipropilenskog konca (PPK) u pogledu prijemčivosti za bakterije i kliničkih karakteristika, uključujući uticaj na zarastanje mekog tkiva u usnoj duplji. Metode U studiju je uključeno deset zdravih ispitanika kod kojih su hirurški izvađeni gornji i donji umnjak sa jedne strane istovremeno, a rane su ušivene različitim koncima (jedan monofilamentni - PPK i jedan polifilamentni - SK). Kvantifikacija bakterija na uzorcima konaca koji su uklonjeni sedam dana posle operacije urađena je metodom lančane reakcije polimeraze u realnom vremenu. Oralni hirurg je uz pomoć Vizuelne analogne skale ocenjivao lakoću intraoperativnog rukovanja, kao i lakoću uklanjanja konaca. Za procenu kvaliteta zarastanja mekog tkiva korišćen je indeks po Landriju. Rezultati Statistički značajno više bakterija nađeno je na svim uzorcima SK u poređenju sa PPK (p = 0,005). PPK se pokazao značajno lakšim za intraoperativno rukovanje u odnosu na SK (p = 0,005). Takođe, zarastanje mekog tkiva, 3. i 7. dana postoperativno, bilo je značajno uspešnije oko PPK nego oko SK (p = 0,016). Neprijatnost zbog prisustva konaca bila je veća kod primene PPK u odnosu na SK, ali bez statistički značajne razlike. Zaključak Polipropilenski konac je u odnosu na svileni konac pokazao značajno manju prijemčivost za bakterije i bolje kliničke karakteristike, uključujući i bolje zarastanje mekog tkiva. PR Projekat Ministarstva nauke Republike Srbije, br. 175075.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Microbial adherence affinity and clinical characteristics of polypropylene versus silk sutures in oral surgery, Svileni i polipropilenski materijal za šavove u oralnoj hirurgiji - kolonizacija mikroorganizmima i kliničke karakteristike",
volume = "146",
number = "5-6",
pages = "258-263",
doi = "10.2298/SARH170428184D"
}

Dragović, M., Pejović, M., Stepić, J., Dragović, S., Nikolić, N., Kuzmanović-Pfićer, J., Čolić, S.,& Milašin, J.. (2018). Microbial adherence affinity and clinical characteristics of polypropylene versus silk sutures in oral surgery. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 146(5-6), 258-263.
https://doi.org/10.2298/SARH170428184D

Dragović M, Pejović M, Stepić J, Dragović S, Nikolić N, Kuzmanović-Pfićer J, Čolić S, Milašin J. Microbial adherence affinity and clinical characteristics of polypropylene versus silk sutures in oral surgery. in Srpski arhiv za celokupno lekarstvo. 2018;146(5-6):258-263.
doi:10.2298/SARH170428184D .

Dragović, Miroslav, Pejović, Marko, Stepić, Jelena, Dragović, Svetlana, Nikolić, Nadja, Kuzmanović-Pfićer, Jovana, Čolić, Snježana, Milašin, Jelena, "Microbial adherence affinity and clinical characteristics of polypropylene versus silk sutures in oral surgery" in Srpski arhiv za celokupno lekarstvo, 146, no. 5-6 (2018):258-263,
https://doi.org/10.2298/SARH170428184D . .

TY  - JOUR
AU  - Simonović, Jelena
AU  - Toljić, Boško
AU  - Nikolić, Nadja
AU  - Perić, Mina
AU  - Vujin, Jasna
AU  - Panajotović, Radmila
AU  - Gajić, Radoš
AU  - Bekyarova, Elena
AU  - Cataldi, Amelia
AU  - Parpura, Vladimir
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2327
AB  - Stem cell-based therapies are considered a promising treatment modality for many medical conditions. Several types of stem cells with variable differentiation potentials have been isolated from dental tissues, among them stem cells from apical papilla (SCAP). In parallel, new classes of biocompatible nanomaterials have also been developed, including graphene and carbon nanotube-based materials. The aim of the study was to assess whether graphene dispersion (GD) and water-soluble single walled carbon nanotubes (ws-SWCNT), may enhance SCAPs capacity to undergo neural differentiation. SCAPs cultivated in neuroinductive medium supplemented with GD and ws-SWCNT, separately and in combination, were subjected to neural marker analysis by real-time polymerase chain reaction (neurofilament medium [NF-M], neurogenin-2 [ngn-2], III-tubulin, microtubule-associated protein 2) and immunocytochemistry (NeuN and III-tubulin). GD, ws-SWCNT, and their combination, had neuro-stimulatory effects on SCAPs, as judged by the production of neural markers. Compared to cells grown in nanomaterial free medium, cells with GD showed higher production of B3T, cells with ws-SWCNT had higher production of ngn-2 and NF-M, while the combination of nanomaterials gave similar levels of both B3T and NF-M as the neuroinductive medium aloal ne, but with the finest neuron-like morphology. In conclusion, GD and ws-SWCNT seem to enhance neurdifferentiation of SCAP.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes
VL  - 106
IS  - 10
SP  - 2653
EP  - 2661
DO  - 10.1002/jbm.a.36461
ER  - 

@article{
author = "Simonović, Jelena and Toljić, Boško and Nikolić, Nadja and Perić, Mina and Vujin, Jasna and Panajotović, Radmila and Gajić, Radoš and Bekyarova, Elena and Cataldi, Amelia and Parpura, Vladimir and Milašin, Jelena",
year = "2018",
abstract = "Stem cell-based therapies are considered a promising treatment modality for many medical conditions. Several types of stem cells with variable differentiation potentials have been isolated from dental tissues, among them stem cells from apical papilla (SCAP). In parallel, new classes of biocompatible nanomaterials have also been developed, including graphene and carbon nanotube-based materials. The aim of the study was to assess whether graphene dispersion (GD) and water-soluble single walled carbon nanotubes (ws-SWCNT), may enhance SCAPs capacity to undergo neural differentiation. SCAPs cultivated in neuroinductive medium supplemented with GD and ws-SWCNT, separately and in combination, were subjected to neural marker analysis by real-time polymerase chain reaction (neurofilament medium [NF-M], neurogenin-2 [ngn-2], III-tubulin, microtubule-associated protein 2) and immunocytochemistry (NeuN and III-tubulin). GD, ws-SWCNT, and their combination, had neuro-stimulatory effects on SCAPs, as judged by the production of neural markers. Compared to cells grown in nanomaterial free medium, cells with GD showed higher production of B3T, cells with ws-SWCNT had higher production of ngn-2 and NF-M, while the combination of nanomaterials gave similar levels of both B3T and NF-M as the neuroinductive medium aloal ne, but with the finest neuron-like morphology. In conclusion, GD and ws-SWCNT seem to enhance neurdifferentiation of SCAP.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes",
volume = "106",
number = "10",
pages = "2653-2661",
doi = "10.1002/jbm.a.36461"
}

Simonović, J., Toljić, B., Nikolić, N., Perić, M., Vujin, J., Panajotović, R., Gajić, R., Bekyarova, E., Cataldi, A., Parpura, V.,& Milašin, J.. (2018). Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 106(10), 2653-2661.
https://doi.org/10.1002/jbm.a.36461

Simonović J, Toljić B, Nikolić N, Perić M, Vujin J, Panajotović R, Gajić R, Bekyarova E, Cataldi A, Parpura V, Milašin J. Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes. in Journal of Biomedical Materials Research Part A. 2018;106(10):2653-2661.
doi:10.1002/jbm.a.36461 .

Simonović, Jelena, Toljić, Boško, Nikolić, Nadja, Perić, Mina, Vujin, Jasna, Panajotović, Radmila, Gajić, Radoš, Bekyarova, Elena, Cataldi, Amelia, Parpura, Vladimir, Milašin, Jelena, "Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes" in Journal of Biomedical Materials Research Part A, 106, no. 10 (2018):2653-2661,
https://doi.org/10.1002/jbm.a.36461 . .

TY  - JOUR
AU  - Nikolić, Nadja
AU  - Čarkić, Jelena
AU  - Ilic-Dimitrijević, Ivana
AU  - Eljabo, Najib
AU  - Radunović, Milena
AU  - Aničić, Boban
AU  - Tanić, Nasta
AU  - Falk, Markus
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2317
AB  - Objective. To investigate the prevalence of p16(INK4) (a), p14(ARF), tumor protein p53 (TP53), and human telomerase reverse transcriptase (hTERT) promoter hypermethylation in mucoepidermoid carcinomas (MECs) and search for a possible association between methylation status and clinicopathological parameters. Study design. DNA extracted from 35 formalin-fixed and paraffin-embedded MEC samples and 10 normal salivary gland (NSG) tissue samples was analyzed for the presence of promoter hypermethylation using methylation-specific polymerase chain reaction testing. Results. The percentages of gene hypermethylation in MECs versus NSGs were the following: p14: 100% versus 20% (P  lt  .001); p16: 60% versus 20% (P = .035); hTERT: 54.3% versus 20% (P = .078); and TP53: 31.4% versus 30% (P = .981). Multiple sites were found to be methylated in 86% of MECs compared with 10% in NSGs (P  lt  .001). TP53 and hTERT were more often methylated in lower clinical stages (P = .033 and P = .005, respectively). Conclusions. Hypermethylation of p14 appears to be an important event in the development of mucoepidermoid carcinoma. High frequency of gene hypermethylation and high incidence of methylation at multiple sites point to the importance of epigenetic phenomena in the pathogenesis of MECs, although with modest impact on clinical parameters.
PB  - Elsevier Science Inc, New York
T2  - Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology
T1  - P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population
VL  - 125
IS  - 1
SP  - 52
EP  - 58
DO  - 10.1016/j.oooo.2017.09.013
ER  - 

@article{
author = "Nikolić, Nadja and Čarkić, Jelena and Ilic-Dimitrijević, Ivana and Eljabo, Najib and Radunović, Milena and Aničić, Boban and Tanić, Nasta and Falk, Markus and Milašin, Jelena",
year = "2018",
abstract = "Objective. To investigate the prevalence of p16(INK4) (a), p14(ARF), tumor protein p53 (TP53), and human telomerase reverse transcriptase (hTERT) promoter hypermethylation in mucoepidermoid carcinomas (MECs) and search for a possible association between methylation status and clinicopathological parameters. Study design. DNA extracted from 35 formalin-fixed and paraffin-embedded MEC samples and 10 normal salivary gland (NSG) tissue samples was analyzed for the presence of promoter hypermethylation using methylation-specific polymerase chain reaction testing. Results. The percentages of gene hypermethylation in MECs versus NSGs were the following: p14: 100% versus 20% (P  lt  .001); p16: 60% versus 20% (P = .035); hTERT: 54.3% versus 20% (P = .078); and TP53: 31.4% versus 30% (P = .981). Multiple sites were found to be methylated in 86% of MECs compared with 10% in NSGs (P  lt  .001). TP53 and hTERT were more often methylated in lower clinical stages (P = .033 and P = .005, respectively). Conclusions. Hypermethylation of p14 appears to be an important event in the development of mucoepidermoid carcinoma. High frequency of gene hypermethylation and high incidence of methylation at multiple sites point to the importance of epigenetic phenomena in the pathogenesis of MECs, although with modest impact on clinical parameters.",
publisher = "Elsevier Science Inc, New York",
journal = "Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology",
title = "P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population",
volume = "125",
number = "1",
pages = "52-58",
doi = "10.1016/j.oooo.2017.09.013"
}

Nikolić, N., Čarkić, J., Ilic-Dimitrijević, I., Eljabo, N., Radunović, M., Aničić, B., Tanić, N., Falk, M.,& Milašin, J.. (2018). P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population. in Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology
Elsevier Science Inc, New York., 125(1), 52-58.
https://doi.org/10.1016/j.oooo.2017.09.013

Nikolić N, Čarkić J, Ilic-Dimitrijević I, Eljabo N, Radunović M, Aničić B, Tanić N, Falk M, Milašin J. P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population. in Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology. 2018;125(1):52-58.
doi:10.1016/j.oooo.2017.09.013 .

Nikolić, Nadja, Čarkić, Jelena, Ilic-Dimitrijević, Ivana, Eljabo, Najib, Radunović, Milena, Aničić, Boban, Tanić, Nasta, Falk, Markus, Milašin, Jelena, "P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population" in Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, 125, no. 1 (2018):52-58,
https://doi.org/10.1016/j.oooo.2017.09.013 . .