TY  - JOUR
AU  - Roganović, Jelena
PY  - 2021
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2585
AB  - The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is able to produce an excessive host immune reaction and may leads to severe disease- a life-threatening condition occurring more often in patients suffering from comorbidities such as hypertension, diabetes and obesity. Infection by human corona viruses highly depends on host microRNA (miR) involved in regulation of host innate immune response and inflammation-modulatory miR-146a is among the first miRs induced by immune reaction to a virus. Moreover, recent analysis showed that miR-146 is predicted to target at the SARS-CoV-2 genome. As the dominant regulator of Toll-like receptors (TLRs) downstream signaling, miR-146a may limit excessive inflammatory response to virus. Downregulation of circulating miR-146a was found in diabetes, obesity and hypertension and it is reflected by enhanced inflammation and fibrosis, systemic effects accompanying severe COVID-19. Thus it could be hypothesized that miR-146a deficiency may contribute to severe COVID-19 state observed in diabetes, obesity and hypertension but further investigations are needed.
PB  - Elsevier
T2  - Medical Hypotheses
T1  - Downregulation of microRNA-146a in diabetes, obesity and hypertension may contribute to severe COVID-19
VL  - 146
SP  - 110448
DO  - 10.1016/j.mehy.2020.110448
ER  - 

@article{
author = "Roganović, Jelena",
year = "2021",
abstract = "The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is able to produce an excessive host immune reaction and may leads to severe disease- a life-threatening condition occurring more often in patients suffering from comorbidities such as hypertension, diabetes and obesity. Infection by human corona viruses highly depends on host microRNA (miR) involved in regulation of host innate immune response and inflammation-modulatory miR-146a is among the first miRs induced by immune reaction to a virus. Moreover, recent analysis showed that miR-146 is predicted to target at the SARS-CoV-2 genome. As the dominant regulator of Toll-like receptors (TLRs) downstream signaling, miR-146a may limit excessive inflammatory response to virus. Downregulation of circulating miR-146a was found in diabetes, obesity and hypertension and it is reflected by enhanced inflammation and fibrosis, systemic effects accompanying severe COVID-19. Thus it could be hypothesized that miR-146a deficiency may contribute to severe COVID-19 state observed in diabetes, obesity and hypertension but further investigations are needed.",
publisher = "Elsevier",
journal = "Medical Hypotheses",
title = "Downregulation of microRNA-146a in diabetes, obesity and hypertension may contribute to severe COVID-19",
volume = "146",
pages = "110448",
doi = "10.1016/j.mehy.2020.110448"
}

Roganović, J.. (2021). Downregulation of microRNA-146a in diabetes, obesity and hypertension may contribute to severe COVID-19. in Medical Hypotheses
Elsevier., 146, 110448.
https://doi.org/10.1016/j.mehy.2020.110448

Roganović J. Downregulation of microRNA-146a in diabetes, obesity and hypertension may contribute to severe COVID-19. in Medical Hypotheses. 2021;146:110448.
doi:10.1016/j.mehy.2020.110448 .

Roganović, Jelena, "Downregulation of microRNA-146a in diabetes, obesity and hypertension may contribute to severe COVID-19" in Medical Hypotheses, 146 (2021):110448,
https://doi.org/10.1016/j.mehy.2020.110448 . .

TY  - JOUR
AU  - Roganović, Jelena
PY  - 2020-12
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2587
AB  - Background: Type 2 diabetes and periodontitis predispose to a higher risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Recent studies show upregulation of innate immuno-regulatory microRNA-146a and -155 in oral fluids of patients with type 2 diabetes as well as of patients with periodontitis. The aim was to investigate whether upregulation of these microRNAs may relate to patient susceptibility to the infection via modulation of SARS-CoV-2 cellular entry factors expression. 
Methods: Due to limited experimental feasibility and health risks in Coronavirus Disease 2019, bioinformatic analyses combining with system biology were used as initial investigation of interaction between microRNA-146 and -155 and genes encoding SARS-CoV-2 entry factors. 
Results: SARS-CoV-2 cellular entry factors are expressed in salivary glands and masticatory mucosa (tongue) at different expression levels, comparable with those measured in lungs and tonsil. MicroRNA-146 and -155 are widely involved in the regulation of SARS-CoV-2 oral cellular entry factors and may enhance expression of ACE2 and modulate genes involved in host immunity. 
Conclusions: Diabetes- and periodontitis-induced increase in microRNA-146a and -155 in oral cavity is predicted to upregulate angiotensin-converting enzyme 2 expression, essential SARS-CoV-2 entry receptors, and modulate host antiviral response. As it could suggest increased infectivity of diabetes and periodontitis patients, additional protective measures for periodontists are recommended.
PB  - American Academy of Periodontology
PB  - John Wiley & Sons, Inc.
T2  - Journal of Periodontology
T1  - MicroRNA-146a and -155, upregulated by periodontitis and type 2 diabetes in oral fluids, are predicted to regulate SARS-CoV-2 oral receptors genes
DO  - 10.1002/JPER.20-0623
ER  - 

@article{
author = "Roganović, Jelena",
year = "2020-12",
abstract = "Background: Type 2 diabetes and periodontitis predispose to a higher risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Recent studies show upregulation of innate immuno-regulatory microRNA-146a and -155 in oral fluids of patients with type 2 diabetes as well as of patients with periodontitis. The aim was to investigate whether upregulation of these microRNAs may relate to patient susceptibility to the infection via modulation of SARS-CoV-2 cellular entry factors expression. 
Methods: Due to limited experimental feasibility and health risks in Coronavirus Disease 2019, bioinformatic analyses combining with system biology were used as initial investigation of interaction between microRNA-146 and -155 and genes encoding SARS-CoV-2 entry factors. 
Results: SARS-CoV-2 cellular entry factors are expressed in salivary glands and masticatory mucosa (tongue) at different expression levels, comparable with those measured in lungs and tonsil. MicroRNA-146 and -155 are widely involved in the regulation of SARS-CoV-2 oral cellular entry factors and may enhance expression of ACE2 and modulate genes involved in host immunity. 
Conclusions: Diabetes- and periodontitis-induced increase in microRNA-146a and -155 in oral cavity is predicted to upregulate angiotensin-converting enzyme 2 expression, essential SARS-CoV-2 entry receptors, and modulate host antiviral response. As it could suggest increased infectivity of diabetes and periodontitis patients, additional protective measures for periodontists are recommended.",
publisher = "American Academy of Periodontology, John Wiley & Sons, Inc.",
journal = "Journal of Periodontology",
title = "MicroRNA-146a and -155, upregulated by periodontitis and type 2 diabetes in oral fluids, are predicted to regulate SARS-CoV-2 oral receptors genes",
doi = "10.1002/JPER.20-0623"
}

Roganović, J.. (2020-12). MicroRNA-146a and -155, upregulated by periodontitis and type 2 diabetes in oral fluids, are predicted to regulate SARS-CoV-2 oral receptors genes. in Journal of Periodontology
American Academy of Periodontology..
https://doi.org/10.1002/JPER.20-0623

Roganović J. MicroRNA-146a and -155, upregulated by periodontitis and type 2 diabetes in oral fluids, are predicted to regulate SARS-CoV-2 oral receptors genes. in Journal of Periodontology. 2020;.
doi:10.1002/JPER.20-0623 .

Roganović, Jelena, "MicroRNA-146a and -155, upregulated by periodontitis and type 2 diabetes in oral fluids, are predicted to regulate SARS-CoV-2 oral receptors genes" in Journal of Periodontology (2020-12),
https://doi.org/10.1002/JPER.20-0623 . .

TY  - JOUR
AU  - Stojić, Vanja
AU  - Glišić, Branislav
AU  - Đukić, Ljiljana
AU  - Prokić, Bogomir
AU  - Janović, Aleksa
AU  - Stamenković, Zorana
AU  - Milutinović-Smiljanić, Sanja
AU  - Danilović, Vesna
AU  - Brković, Božidar
AU  - Roganović, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2512
AB  - Objective: We aimed to investigate alteration in cellular signaling mediated by vascular endothelial growth factor (VEGF) and parameters of oxidative stress/nitric oxide generation, superoxide dismutase (SOD) and neuronal nitric oxide synthase (nNOS), underlying altered functional mechanical loading of TMJ (temporomandibular joint) during lateral mandibular deviation. Design: Thirty-eight 5-week-old male Wistar rats were divided into experimental group, which received acrylic resin appliance that shifted mandible to the left during closure, and control group. Computed tomography and histomorphometry were used for condyle analyses, while samples of condyle, synovial membrane and m. masseter were analyzed with enzyme-linked immunosorbent assay and spectrophotometry to determine VEGF and nNOS protein concentrations, and SOD activity. Results: Experimental group of rats developed smaller and asymmetrical mandibles. Less of new bone and cartilage formation and larger bone marrow cavities area were found in the experimental group. Higher VEGF expression in condyle and m. masseter as well as higher nNOS expression in m. masseter and synovial membrane were found in the experimental compared to the control group. Alteration of SOD activity was found in m. masseter and synovial membrane in the experimental group. Conclusions: Lateral mandibular deviation induces mandibular and condylar morphological changes as well as significant cellular signaling alterations in condyle, synovial membrane and masticatory muscle. Cellular VEGF protein overexpression and oxidative stress/nitric oxide disbalance could be the mechanisms underlying unbalanced functional TMJ loading due to mandibular deviation.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Archives of Oral Biology
T1  - Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle
VL  - 110
DO  - 10.1016/j.archoralbio.2019.104599
ER  - 

@article{
author = "Stojić, Vanja and Glišić, Branislav and Đukić, Ljiljana and Prokić, Bogomir and Janović, Aleksa and Stamenković, Zorana and Milutinović-Smiljanić, Sanja and Danilović, Vesna and Brković, Božidar and Roganović, Jelena",
year = "2020",
abstract = "Objective: We aimed to investigate alteration in cellular signaling mediated by vascular endothelial growth factor (VEGF) and parameters of oxidative stress/nitric oxide generation, superoxide dismutase (SOD) and neuronal nitric oxide synthase (nNOS), underlying altered functional mechanical loading of TMJ (temporomandibular joint) during lateral mandibular deviation. Design: Thirty-eight 5-week-old male Wistar rats were divided into experimental group, which received acrylic resin appliance that shifted mandible to the left during closure, and control group. Computed tomography and histomorphometry were used for condyle analyses, while samples of condyle, synovial membrane and m. masseter were analyzed with enzyme-linked immunosorbent assay and spectrophotometry to determine VEGF and nNOS protein concentrations, and SOD activity. Results: Experimental group of rats developed smaller and asymmetrical mandibles. Less of new bone and cartilage formation and larger bone marrow cavities area were found in the experimental group. Higher VEGF expression in condyle and m. masseter as well as higher nNOS expression in m. masseter and synovial membrane were found in the experimental compared to the control group. Alteration of SOD activity was found in m. masseter and synovial membrane in the experimental group. Conclusions: Lateral mandibular deviation induces mandibular and condylar morphological changes as well as significant cellular signaling alterations in condyle, synovial membrane and masticatory muscle. Cellular VEGF protein overexpression and oxidative stress/nitric oxide disbalance could be the mechanisms underlying unbalanced functional TMJ loading due to mandibular deviation.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Archives of Oral Biology",
title = "Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle",
volume = "110",
doi = "10.1016/j.archoralbio.2019.104599"
}

Stojić, V., Glišić, B., Đukić, L., Prokić, B., Janović, A., Stamenković, Z., Milutinović-Smiljanić, S., Danilović, V., Brković, B.,& Roganović, J.. (2020). Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle. in Archives of Oral Biology
Pergamon-Elsevier Science Ltd, Oxford., 110.
https://doi.org/10.1016/j.archoralbio.2019.104599

Stojić V, Glišić B, Đukić L, Prokić B, Janović A, Stamenković Z, Milutinović-Smiljanić S, Danilović V, Brković B, Roganović J. Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle. in Archives of Oral Biology. 2020;110.
doi:10.1016/j.archoralbio.2019.104599 .

Stojić, Vanja, Glišić, Branislav, Đukić, Ljiljana, Prokić, Bogomir, Janović, Aleksa, Stamenković, Zorana, Milutinović-Smiljanić, Sanja, Danilović, Vesna, Brković, Božidar, Roganović, Jelena, "Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle" in Archives of Oral Biology, 110 (2020),
https://doi.org/10.1016/j.archoralbio.2019.104599 . .

TY  - JOUR
AU  - Roganović, Jelena
AU  - Radenković, Miroslav
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2586
PB  - John Wiley & Sons, Inc.
T2  - Oral Diseases
T1  - Letter to the Editor: Diabetes, obesity and hypertension may promote oral SARS-CoV-2 infection-Salivary soluble ACE2 perspective.
SP  - 1
EP  - 3
DO  - 10.1111/odi.13711
ER  - 

@article{
author = "Roganović, Jelena and Radenković, Miroslav",
year = "2020",
publisher = "John Wiley & Sons, Inc.",
journal = "Oral Diseases",
title = "Letter to the Editor: Diabetes, obesity and hypertension may promote oral SARS-CoV-2 infection-Salivary soluble ACE2 perspective.",
pages = "1-3",
doi = "10.1111/odi.13711"
}

Roganović, J.,& Radenković, M.. (2020). Letter to the Editor: Diabetes, obesity and hypertension may promote oral SARS-CoV-2 infection-Salivary soluble ACE2 perspective.. in Oral Diseases
John Wiley & Sons, Inc.., 1-3.
https://doi.org/10.1111/odi.13711

Roganović J, Radenković M. Letter to the Editor: Diabetes, obesity and hypertension may promote oral SARS-CoV-2 infection-Salivary soluble ACE2 perspective.. in Oral Diseases. 2020;:1-3.
doi:10.1111/odi.13711 .

Roganović, Jelena, Radenković, Miroslav, "Letter to the Editor: Diabetes, obesity and hypertension may promote oral SARS-CoV-2 infection-Salivary soluble ACE2 perspective." in Oral Diseases (2020):1-3,
https://doi.org/10.1111/odi.13711 . .

TY  - JOUR
AU  - Ilić, Jugoslav
AU  - Radović, Katarina
AU  - Brković, Božidar
AU  - Vasić, Jugoslav
AU  - Roganović, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2588
AB  - Introduction/Objective. We aimed to investigate the effects of diabetes mellitus (DM) on rat dental pulp repair by measuring time-dependent changes in expressions of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP 2) following direct pulp capping. Methods. Two groups, each of 20 Wistar rats, received either streptozotocin (for DM induction) or the same volume of sterile saline. A week later, the pulp of maxillary and mandibular right incisors in diabetic and non-diabetic groups were exposed and capped with calcium hydroxide in order to provoke reparative response. The levels of VEGF and BMP 2 were determined in the pulp tissue lysates one and seven days after the pulp capping, using enzyme-linked immunosorbent assays. Results. Diabetic state per se increased VEGF level, with a peak at first day after the pulp capping (19.3 ± 0.9 pg/mg, p < 0.001), but did not affect BMP 2 levels. Significant increase of BMP 2 expression was noticed on the seventh day in capped pulp, but only in diabetic rats (16.7 ± 1 pg/mg, p = 0.001). Positive correlation between VEGF and BMP 2 was found on the seventh day following capping, only in diabetic pulp (r = 0.905, p = 0.003). Conclusion. Diabetes-induced increase in VEGF expression reflects changes in the inflammatory phase of pulp repair in DM. Increase in BMP 2 expression suggest that stimulating effect of calcium hydroxide appears seven days after diabetic pulp capping.
AB  - Uvod/Cilj Cilj ove studije bio je da se ispita efekat dijabetesa melitusa na reparaciju zubne pulpe pacova utvrđivanjem vremenski zavisnih promena u ekspresiji vaskularnog endotelnog faktora rasta (VEGF) i kostnog morfogenetskog proteina 2 (BMP 2) posle direktnog prekrivanja pulpe. Metode Istraživanje je sprovedeno na pacovima soja vistar, podeljenim u dve grupe od po 20 životinja, pri čemu je jedna grupa dobila streptozotocin (za indukciju dijabetesa melitusa), a druga sterilni fiziološki rastvor u istoj zapremini. Posle nedelju dana pulpe maksilarnih i mandibularnih donjih inciziva kod dijabetičnih i nedijabetičnih životinja su eksponirane i odmah zatim prekrivene kalcijum-hidroksidom da bi se izazvao reparatorni odgovor. Nivoi VEGF i BMP 2 su utvrđivani u lizatima pulpnog tkiva, prvog i sedmog dana posle direktnog prekrivanja imunoenzimskim testom ELISA. Rezultati Dijabetično stanje je dovelo do rasta nivoa VEGF, sa maksimumom utvrđenim prvog dana posle prekrivanja pulpe (19,3 ± 0,9 pg/mg, p < 0,001), ali nije uticalo na nivoe BMP 2. Značajan porast BMP 2 je utvrđen sedmog dana posle prekrivanja pulpe, ali samo kod dijabetičnih pacova (16,7 ± 1 pg/mg, p = 0,001). Kod ovih životinja u istom periodu posle prekrivanja nađena je pozitivna korelacija između nivoa VEGF i BMP 2 (r = 0,905, p = 0,003). Zaključak Dijabetesom indukovan porast ekspresije VEGF ukazuje na promene u inflamatornoj fazi pulpne reparacije. Porast ekspresije BMP 2 ukazuje da se stimulativan reparatorni efekat kalcijum-hidroksida javlja sedmog dana posle prekrivanja dijabetične pulpe.
PB  - Beograd: Srpsko lekarsko društvo
T2  - Srpski arhiv za celokupno lekarstvo
T1  - The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2
T1  - Reparacija dijabetične zubne pulpe – uloga vaskularnog endotelnog faktora rasta i kostnog morfogenetskog proteina 2
VL  - 148
IS  - 11-12
SP  - 673
EP  - 678
DO  - 10.2298/SARH200228057I
ER  - 

@article{
author = "Ilić, Jugoslav and Radović, Katarina and Brković, Božidar and Vasić, Jugoslav and Roganović, Jelena",
year = "2020",
abstract = "Introduction/Objective. We aimed to investigate the effects of diabetes mellitus (DM) on rat dental pulp repair by measuring time-dependent changes in expressions of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP 2) following direct pulp capping. Methods. Two groups, each of 20 Wistar rats, received either streptozotocin (for DM induction) or the same volume of sterile saline. A week later, the pulp of maxillary and mandibular right incisors in diabetic and non-diabetic groups were exposed and capped with calcium hydroxide in order to provoke reparative response. The levels of VEGF and BMP 2 were determined in the pulp tissue lysates one and seven days after the pulp capping, using enzyme-linked immunosorbent assays. Results. Diabetic state per se increased VEGF level, with a peak at first day after the pulp capping (19.3 ± 0.9 pg/mg, p < 0.001), but did not affect BMP 2 levels. Significant increase of BMP 2 expression was noticed on the seventh day in capped pulp, but only in diabetic rats (16.7 ± 1 pg/mg, p = 0.001). Positive correlation between VEGF and BMP 2 was found on the seventh day following capping, only in diabetic pulp (r = 0.905, p = 0.003). Conclusion. Diabetes-induced increase in VEGF expression reflects changes in the inflammatory phase of pulp repair in DM. Increase in BMP 2 expression suggest that stimulating effect of calcium hydroxide appears seven days after diabetic pulp capping., Uvod/Cilj Cilj ove studije bio je da se ispita efekat dijabetesa melitusa na reparaciju zubne pulpe pacova utvrđivanjem vremenski zavisnih promena u ekspresiji vaskularnog endotelnog faktora rasta (VEGF) i kostnog morfogenetskog proteina 2 (BMP 2) posle direktnog prekrivanja pulpe. Metode Istraživanje je sprovedeno na pacovima soja vistar, podeljenim u dve grupe od po 20 životinja, pri čemu je jedna grupa dobila streptozotocin (za indukciju dijabetesa melitusa), a druga sterilni fiziološki rastvor u istoj zapremini. Posle nedelju dana pulpe maksilarnih i mandibularnih donjih inciziva kod dijabetičnih i nedijabetičnih životinja su eksponirane i odmah zatim prekrivene kalcijum-hidroksidom da bi se izazvao reparatorni odgovor. Nivoi VEGF i BMP 2 su utvrđivani u lizatima pulpnog tkiva, prvog i sedmog dana posle direktnog prekrivanja imunoenzimskim testom ELISA. Rezultati Dijabetično stanje je dovelo do rasta nivoa VEGF, sa maksimumom utvrđenim prvog dana posle prekrivanja pulpe (19,3 ± 0,9 pg/mg, p < 0,001), ali nije uticalo na nivoe BMP 2. Značajan porast BMP 2 je utvrđen sedmog dana posle prekrivanja pulpe, ali samo kod dijabetičnih pacova (16,7 ± 1 pg/mg, p = 0,001). Kod ovih životinja u istom periodu posle prekrivanja nađena je pozitivna korelacija između nivoa VEGF i BMP 2 (r = 0,905, p = 0,003). Zaključak Dijabetesom indukovan porast ekspresije VEGF ukazuje na promene u inflamatornoj fazi pulpne reparacije. Porast ekspresije BMP 2 ukazuje da se stimulativan reparatorni efekat kalcijum-hidroksida javlja sedmog dana posle prekrivanja dijabetične pulpe.",
publisher = "Beograd: Srpsko lekarsko društvo",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2, Reparacija dijabetične zubne pulpe – uloga vaskularnog endotelnog faktora rasta i kostnog morfogenetskog proteina 2",
volume = "148",
number = "11-12",
pages = "673-678",
doi = "10.2298/SARH200228057I"
}

Ilić, J., Radović, K., Brković, B., Vasić, J.,& Roganović, J.. (2020). The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2. in Srpski arhiv za celokupno lekarstvo
Beograd: Srpsko lekarsko društvo., 148(11-12), 673-678.
https://doi.org/10.2298/SARH200228057I

Ilić J, Radović K, Brković B, Vasić J, Roganović J. The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2. in Srpski arhiv za celokupno lekarstvo. 2020;148(11-12):673-678.
doi:10.2298/SARH200228057I .

Ilić, Jugoslav, Radović, Katarina, Brković, Božidar, Vasić, Jugoslav, Roganović, Jelena, "The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2" in Srpski arhiv za celokupno lekarstvo, 148, no. 11-12 (2020):673-678,
https://doi.org/10.2298/SARH200228057I . .

TY  - JOUR
AU  - Vučetić, Milan
AU  - Roganović, Jelena
AU  - Freilich, M.
AU  - Shafer, D.
AU  - Milić, Marija
AU  - Đukić, Ljiljana
AU  - Petrović, Nina
AU  - Marković, Evgenija
AU  - Marković, Aleksa
AU  - Brković, Božidar
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2481
AB  - Objective: To evaluate an association between bone levels of inflammation/oxidative stress mediators and postoperative discomfort after third molar conventional or piezosurgery. Material and methods: Twenty-six subjects with bilaterally impacted mandibular third molars, who underwent either piezo or conventional surgery, were included in a split-mouth design study. MicroRNA-21 (miR-21) expression, interleukin-1 beta (IL-1β), and vascular endothelial growth factor (VEGF) proteins, as well as superoxide dismutase (SOD) activity in alveolar bone, were evaluated. Pain intensity, the first pain appearance, analgesic first use and total dose taken, trismus, and swelling were clinically recorded. Results: MiR-21 expression was higher while VEGF protein was lower in piezosurgery vs. conventional groups. The differences in IL-1β protein and SOD activity were not significant between groups. The pain intensity on the first day was significantly decreased in piezosurgery group. The first pain appearance and the first analgesic taken were reported sooner in conventional vs. piezosurgical group. Significantly pronounced trismus on the third day following conventional surgery was found. In conventional group, significantly increased trismus was observed on the third compared to the first postoperative day. MiR-21 showed significant correlation with the first pain appearance. Conclusion: Delayed onset of less pronounced postoperative pain after piezosurgical vs. conventional extraction of impacted lower third molar was significantly associated with expression of bone miR-21. Clinical relevance: Alveolar bone miR-21 may reflect surgical stress and is associated with third molar postoperative pain onset.
PB  - Springer
T2  - Clinical Oral Investigations
T1  - Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort
DO  - 10.1007/s00784-020-03366-6
ER  - 

@article{
author = "Vučetić, Milan and Roganović, Jelena and Freilich, M. and Shafer, D. and Milić, Marija and Đukić, Ljiljana and Petrović, Nina and Marković, Evgenija and Marković, Aleksa and Brković, Božidar",
year = "2020",
abstract = "Objective: To evaluate an association between bone levels of inflammation/oxidative stress mediators and postoperative discomfort after third molar conventional or piezosurgery. Material and methods: Twenty-six subjects with bilaterally impacted mandibular third molars, who underwent either piezo or conventional surgery, were included in a split-mouth design study. MicroRNA-21 (miR-21) expression, interleukin-1 beta (IL-1β), and vascular endothelial growth factor (VEGF) proteins, as well as superoxide dismutase (SOD) activity in alveolar bone, were evaluated. Pain intensity, the first pain appearance, analgesic first use and total dose taken, trismus, and swelling were clinically recorded. Results: MiR-21 expression was higher while VEGF protein was lower in piezosurgery vs. conventional groups. The differences in IL-1β protein and SOD activity were not significant between groups. The pain intensity on the first day was significantly decreased in piezosurgery group. The first pain appearance and the first analgesic taken were reported sooner in conventional vs. piezosurgical group. Significantly pronounced trismus on the third day following conventional surgery was found. In conventional group, significantly increased trismus was observed on the third compared to the first postoperative day. MiR-21 showed significant correlation with the first pain appearance. Conclusion: Delayed onset of less pronounced postoperative pain after piezosurgical vs. conventional extraction of impacted lower third molar was significantly associated with expression of bone miR-21. Clinical relevance: Alveolar bone miR-21 may reflect surgical stress and is associated with third molar postoperative pain onset.",
publisher = "Springer",
journal = "Clinical Oral Investigations",
title = "Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort",
doi = "10.1007/s00784-020-03366-6"
}

Vučetić, M., Roganović, J., Freilich, M., Shafer, D., Milić, M., Đukić, L., Petrović, N., Marković, E., Marković, A.,& Brković, B.. (2020). Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort. in Clinical Oral Investigations
Springer..
https://doi.org/10.1007/s00784-020-03366-6

Vučetić M, Roganović J, Freilich M, Shafer D, Milić M, Đukić L, Petrović N, Marković E, Marković A, Brković B. Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort. in Clinical Oral Investigations. 2020;.
doi:10.1007/s00784-020-03366-6 .

Vučetić, Milan, Roganović, Jelena, Freilich, M., Shafer, D., Milić, Marija, Đukić, Ljiljana, Petrović, Nina, Marković, Evgenija, Marković, Aleksa, Brković, Božidar, "Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort" in Clinical Oral Investigations (2020),
https://doi.org/10.1007/s00784-020-03366-6 . .

TY  - JOUR
AU  - Kopčalić, Katarina
AU  - Petrović, Nina
AU  - Stanojković, Tatjana P.
AU  - Stanković, Vesna
AU  - Bukumirić, Zoran
AU  - Roganović, Jelena
AU  - Mališić, Emina
AU  - Nikitović, Marina
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2594
AB  - Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. 
Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative ΔΔCt method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. 
Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). 
Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs.
PB  - Elsevier GmbH
T2  - Pathology - Research and Practice
T1  - Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study
VL  - 215
IS  - 4
SP  - 626
EP  - 631
DO  - 10.1016/j.prp.2018.12.007
ER  - 

@article{
author = "Kopčalić, Katarina and Petrović, Nina and Stanojković, Tatjana P. and Stanković, Vesna and Bukumirić, Zoran and Roganović, Jelena and Mališić, Emina and Nikitović, Marina",
year = "2019",
abstract = "Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. 
Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative ΔΔCt method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. 
Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). 
Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs.",
publisher = "Elsevier GmbH",
journal = "Pathology - Research and Practice",
title = "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study",
volume = "215",
number = "4",
pages = "626-631",
doi = "10.1016/j.prp.2018.12.007"
}

Kopčalić, K., Petrović, N., Stanojković, T. P., Stanković, V., Bukumirić, Z., Roganović, J., Mališić, E.,& Nikitović, M.. (2019). Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology - Research and Practice
Elsevier GmbH., 215(4), 626-631.
https://doi.org/10.1016/j.prp.2018.12.007

Kopčalić K, Petrović N, Stanojković TP, Stanković V, Bukumirić Z, Roganović J, Mališić E, Nikitović M. Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology - Research and Practice. 2019;215(4):626-631.
doi:10.1016/j.prp.2018.12.007 .

Kopčalić, Katarina, Petrović, Nina, Stanojković, Tatjana P., Stanković, Vesna, Bukumirić, Zoran, Roganović, Jelena, Mališić, Emina, Nikitović, Marina, "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study" in Pathology - Research and Practice, 215, no. 4 (2019):626-631,
https://doi.org/10.1016/j.prp.2018.12.007 . .

TY  - JOUR
AU  - Krunić, Jelena
AU  - Stojanović, Nikola
AU  - Đukić, Ljiljana
AU  - Roganović, Jelena
AU  - Popović, Branka
AU  - Simić, Ivana
AU  - Stojić, Dragica
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2465
AB  - ObjectivesTo evaluate local effect of gaseous ozone on bacteria in deep carious lesions after incomplete caries removal, using chlorhexidine as control, and to investigate its effect on pulp vascular endothelial growth factor (VEGF), neuronal nitric oxide synthase (nNOS), and superoxide dismutase (SOD).Materials and methodsAntibacterial effect was evaluated in 48 teeth with diagnosed deep carious lesion. After incomplete caries removal, teeth were randomly allocated into two groups regarding the cavity disinfectant used: ozone (open system) or 2% chlorhexidine. Dentin samples were analyzed for the presence of total bacteria and Lactobacillus spp. by real-time quantitative polymerase chain reaction. For evaluation of ozone effect on dental pulp, 38 intact permanent teeth indicated for pulp removal/tooth extraction were included. After cavity preparation, teeth were randomly allocated into two groups: ozone group and control group. VEGF/nNOS level and SOD activity in dental pulp were determined by enzyme-linked immunosorbent assay and spectrophotometric method, respectively.ResultsOzone application decreased number of total bacteria (p=0.001) and Lactobacillus spp. (p lt 0.001), similarly to chlorhexidine. The VEGF (p lt 0.001) and nNOS (p=0.012) levels in dental pulp after ozone application were higher, while SOD activity was lower (p=0.001) comparing to those in control pulp.ConclusionsAntibacterial effect of ozone on residual bacteria after incomplete caries removal was similar to that of 2% chlorhexidine. Effect of ozone on pulp VEGF, nNOS, and SOD indicated its biocompatibility.Clinical relevanceOzone appears as effective and biocompatible cavity disinfectant in treatment of deep carious lesions by incomplete caries removal technique.
PB  - Springer Heidelberg, Heidelberg
T2  - Clinical Oral Investigations
T1  - Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal
VL  - 23
IS  - 2
SP  - 785
EP  - 792
DO  - 10.1007/s00784-018-2495-x
ER  - 

@article{
author = "Krunić, Jelena and Stojanović, Nikola and Đukić, Ljiljana and Roganović, Jelena and Popović, Branka and Simić, Ivana and Stojić, Dragica",
year = "2019",
abstract = "ObjectivesTo evaluate local effect of gaseous ozone on bacteria in deep carious lesions after incomplete caries removal, using chlorhexidine as control, and to investigate its effect on pulp vascular endothelial growth factor (VEGF), neuronal nitric oxide synthase (nNOS), and superoxide dismutase (SOD).Materials and methodsAntibacterial effect was evaluated in 48 teeth with diagnosed deep carious lesion. After incomplete caries removal, teeth were randomly allocated into two groups regarding the cavity disinfectant used: ozone (open system) or 2% chlorhexidine. Dentin samples were analyzed for the presence of total bacteria and Lactobacillus spp. by real-time quantitative polymerase chain reaction. For evaluation of ozone effect on dental pulp, 38 intact permanent teeth indicated for pulp removal/tooth extraction were included. After cavity preparation, teeth were randomly allocated into two groups: ozone group and control group. VEGF/nNOS level and SOD activity in dental pulp were determined by enzyme-linked immunosorbent assay and spectrophotometric method, respectively.ResultsOzone application decreased number of total bacteria (p=0.001) and Lactobacillus spp. (p lt 0.001), similarly to chlorhexidine. The VEGF (p lt 0.001) and nNOS (p=0.012) levels in dental pulp after ozone application were higher, while SOD activity was lower (p=0.001) comparing to those in control pulp.ConclusionsAntibacterial effect of ozone on residual bacteria after incomplete caries removal was similar to that of 2% chlorhexidine. Effect of ozone on pulp VEGF, nNOS, and SOD indicated its biocompatibility.Clinical relevanceOzone appears as effective and biocompatible cavity disinfectant in treatment of deep carious lesions by incomplete caries removal technique.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Clinical Oral Investigations",
title = "Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal",
volume = "23",
number = "2",
pages = "785-792",
doi = "10.1007/s00784-018-2495-x"
}

Krunić, J., Stojanović, N., Đukić, L., Roganović, J., Popović, B., Simić, I.,& Stojić, D.. (2019). Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal. in Clinical Oral Investigations
Springer Heidelberg, Heidelberg., 23(2), 785-792.
https://doi.org/10.1007/s00784-018-2495-x

Krunić J, Stojanović N, Đukić L, Roganović J, Popović B, Simić I, Stojić D. Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal. in Clinical Oral Investigations. 2019;23(2):785-792.
doi:10.1007/s00784-018-2495-x .

Krunić, Jelena, Stojanović, Nikola, Đukić, Ljiljana, Roganović, Jelena, Popović, Branka, Simić, Ivana, Stojić, Dragica, "Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal" in Clinical Oral Investigations, 23, no. 2 (2019):785-792,
https://doi.org/10.1007/s00784-018-2495-x . .

TY  - JOUR
AU  - Kopcalić, Katarina
AU  - Petrović, Nina
AU  - Stanojković, Tatjana P.
AU  - Stanković, Vesna
AU  - Bukumirić, Zoran
AU  - Roganović, Jelena
AU  - Malisić, Emina
AU  - Nikitović, Marina
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2391
AB  - Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative Delta Delta Ct method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs.
PB  - Elsevier Gmbh, Munich
T2  - Pathology Research & Practice
T1  - Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study
VL  - 215
IS  - 4
SP  - 626
EP  - 631
DO  - 10.1016/j.prp.2018.12.007
ER  - 

@article{
author = "Kopcalić, Katarina and Petrović, Nina and Stanojković, Tatjana P. and Stanković, Vesna and Bukumirić, Zoran and Roganović, Jelena and Malisić, Emina and Nikitović, Marina",
year = "2019",
abstract = "Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative Delta Delta Ct method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs.",
publisher = "Elsevier Gmbh, Munich",
journal = "Pathology Research & Practice",
title = "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study",
volume = "215",
number = "4",
pages = "626-631",
doi = "10.1016/j.prp.2018.12.007"
}

Kopcalić, K., Petrović, N., Stanojković, T. P., Stanković, V., Bukumirić, Z., Roganović, J., Malisić, E.,& Nikitović, M.. (2019). Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology Research & Practice
Elsevier Gmbh, Munich., 215(4), 626-631.
https://doi.org/10.1016/j.prp.2018.12.007

Kopcalić K, Petrović N, Stanojković TP, Stanković V, Bukumirić Z, Roganović J, Malisić E, Nikitović M. Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology Research & Practice. 2019;215(4):626-631.
doi:10.1016/j.prp.2018.12.007 .

Kopcalić, Katarina, Petrović, Nina, Stanojković, Tatjana P., Stanković, Vesna, Bukumirić, Zoran, Roganović, Jelena, Malisić, Emina, Nikitović, Marina, "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study" in Pathology Research & Practice, 215, no. 4 (2019):626-631,
https://doi.org/10.1016/j.prp.2018.12.007 . .

TY  - JOUR
AU  - Baćević, Miljana
AU  - Brković, Božidar
AU  - Lamber, France
AU  - Đukić, Ljiljana
AU  - Petrović, Nina
AU  - Roganović, Jelena
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2388
AB  - Objective: Leukocyte- and platelet-rich fibrin (L-PRF) represents a natural, low-cost product which may promote tissue healing by mechanisms not fully elucidated. Diabetes mellitus (DM) disrupts bone healing by inducing inflammation and oxidative stress (OS), mechanisms regulated by microRNAs (miRs). The aim of the present study was to investigate the microRNA-21 (miR-21) involvement in diabetic bone regeneration using L-PRF alone or in combination with a standard grafting material. Design: After the induction of diabetes (alloxan 100 mg/kg), four cranial osteotomies were made in diabetic (n = 12) and non-diabetic (n = 12) rabbits: one was left empty and the remaining three were grafted with L-PRF, bovine hydroxyapatite (Bio-Oss((R))) and L-PRF + Bio-Oss((R)). Two and eight weeks postoperatively, the samples were harvested for miR-21 expression (Real-time RT-PCR) and enzyme-linked immunosorbent assay analyses. Results: Diabetic rabbits showed decreased miR-21 and matrix metalloproteinase-9 (MMP-9) protein expression while increased malondialdehyde (MDA) levels two weeks postoperatively; however, there were no significant differences in miR-21 and MMP-9 levels between diabetic and non-diabetic rabbits in samples taken eight weeks postoperatively. Application of L-PRF and L-PRF + Bio-Oss((R)) improved miR-21 and MMP-9 and decreased MDA levels while Bio-Oss((R)) alone enhanced superoxide dismutase (SOD) activity levels in diabetic rabbits. Conclusion: L-PRF alone or in combination with bovine hydroxyapatite as bone graft could be beneficial in DM since it seems to improve inflammation-modulatory miR-21 expression and decreases oxidative stress.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Archives of Oral Biology
T1  - Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits
VL  - 102
SP  - 231
EP  - 237
DO  - 10.1016/j.archoralbio.2019.05.005
ER  - 

@article{
author = "Baćević, Miljana and Brković, Božidar and Lamber, France and Đukić, Ljiljana and Petrović, Nina and Roganović, Jelena",
year = "2019",
abstract = "Objective: Leukocyte- and platelet-rich fibrin (L-PRF) represents a natural, low-cost product which may promote tissue healing by mechanisms not fully elucidated. Diabetes mellitus (DM) disrupts bone healing by inducing inflammation and oxidative stress (OS), mechanisms regulated by microRNAs (miRs). The aim of the present study was to investigate the microRNA-21 (miR-21) involvement in diabetic bone regeneration using L-PRF alone or in combination with a standard grafting material. Design: After the induction of diabetes (alloxan 100 mg/kg), four cranial osteotomies were made in diabetic (n = 12) and non-diabetic (n = 12) rabbits: one was left empty and the remaining three were grafted with L-PRF, bovine hydroxyapatite (Bio-Oss((R))) and L-PRF + Bio-Oss((R)). Two and eight weeks postoperatively, the samples were harvested for miR-21 expression (Real-time RT-PCR) and enzyme-linked immunosorbent assay analyses. Results: Diabetic rabbits showed decreased miR-21 and matrix metalloproteinase-9 (MMP-9) protein expression while increased malondialdehyde (MDA) levels two weeks postoperatively; however, there were no significant differences in miR-21 and MMP-9 levels between diabetic and non-diabetic rabbits in samples taken eight weeks postoperatively. Application of L-PRF and L-PRF + Bio-Oss((R)) improved miR-21 and MMP-9 and decreased MDA levels while Bio-Oss((R)) alone enhanced superoxide dismutase (SOD) activity levels in diabetic rabbits. Conclusion: L-PRF alone or in combination with bovine hydroxyapatite as bone graft could be beneficial in DM since it seems to improve inflammation-modulatory miR-21 expression and decreases oxidative stress.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Archives of Oral Biology",
title = "Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits",
volume = "102",
pages = "231-237",
doi = "10.1016/j.archoralbio.2019.05.005"
}

Baćević, M., Brković, B., Lamber, F., Đukić, L., Petrović, N.,& Roganović, J.. (2019). Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits. in Archives of Oral Biology
Pergamon-Elsevier Science Ltd, Oxford., 102, 231-237.
https://doi.org/10.1016/j.archoralbio.2019.05.005

Baćević M, Brković B, Lamber F, Đukić L, Petrović N, Roganović J. Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits. in Archives of Oral Biology. 2019;102:231-237.
doi:10.1016/j.archoralbio.2019.05.005 .

Baćević, Miljana, Brković, Božidar, Lamber, France, Đukić, Ljiljana, Petrović, Nina, Roganović, Jelena, "Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits" in Archives of Oral Biology, 102 (2019):231-237,
https://doi.org/10.1016/j.archoralbio.2019.05.005 . .

TY  - JOUR
AU  - Milosavljević, A.
AU  - Đukić, Ljiljana
AU  - Toljić, Boško
AU  - Milašin, Jelena
AU  - Dželetović, Bojan
AU  - Brković, Božidar
AU  - Roganović, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2321
AB  - AimTo investigate melatonin (MEL) levels in human dental pulp tissue (hDP) in type 2 diabetic (T2D) participants and the underlying molecular mechanisms of its effects in human dental pulp cells (hDPCs) under hyperglycaemia. MethodologyThe study included 16 healthy and 16 T2D participants who underwent vital pulp extirpation for hDP and four healthy participants undergoing third molar extraction for hDPCs analyses. MTT and NRU were used as tests for cytotoxicity. The pulp tissue levels of MEL, inducible NO synthase (iNOS) and superoxide dismutase (SOD) activity, as well as iNOS, histone acetyltransferase p300 (p300) and SOD activity levels in hDPCs incubated with MEL (0.1 and 1.0mmolL(-1)) under normoglycaemia and hyperglycaemia were measured by enzyme-linked immunosorbent assay. Comparisons between the two groups were made by unpaired t-tests or Mann-Whitney test whilst the chi-square test was used for dichotomous variables. To compare more groups, the Kruskal-Wallis test with Dunn's multiple comparison was used, whilst Spearman correlation was used to assess association between two variables. ResultsMelatonin was decreased (124.3021.6 vs. 240.0 +/- 19.1 pgmL(-1), P lt 0.01), whilst iNOS levels increased (0.92 +/- 0.08 vs. 0.32 +/- 0.09ngmL(-1), P lt 0.01) in hDP from T2D compared to nondiabetic participants. In hDPCs, MEL (0.1 and 1.0mmolL(-1)) had no cytotoxicity. Incubation with 1.0mmolL(-1) of MEL (24h) decreased hyperglycaemia-induced increases of iNOS (0.34 +/- 0.01ngmL(-1) vs. 0.40 +/- 0.01ngmL(-1), P lt 0.01) and p300 (11.59 +/- 0.58ngmL(-1) vs. 16.12 +/- 0.39ngmL(-1), P lt 0.01), and also, increased SOD activity (87.11 +/- 3.10% vs. 68.56 +/- 3.77%, P lt 0.01) to the levels comparable to the normoglycaemic; iNOS and p300 protein expression levels showed strong positive correlation under hyperglycaemia (Spearman r=0.8242, P lt 0.001). ConclusionType 2 diabetic participants had decreased MEL in hDP. At pharmacological concentrations, MEL is not cytotoxic for hDPCs and normalizes iNOS and SOD activity levels in hyperglyceamic hDPCs suggesting its antioxidant and protective effects in human dental pulp tissue under hyperglycaemia.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions
VL  - 51
IS  - 10
SP  - 1149
EP  - 1158
DO  - 10.1111/iej.12934
ER  - 

@article{
author = "Milosavljević, A. and Đukić, Ljiljana and Toljić, Boško and Milašin, Jelena and Dželetović, Bojan and Brković, Božidar and Roganović, Jelena",
year = "2018",
abstract = "AimTo investigate melatonin (MEL) levels in human dental pulp tissue (hDP) in type 2 diabetic (T2D) participants and the underlying molecular mechanisms of its effects in human dental pulp cells (hDPCs) under hyperglycaemia. MethodologyThe study included 16 healthy and 16 T2D participants who underwent vital pulp extirpation for hDP and four healthy participants undergoing third molar extraction for hDPCs analyses. MTT and NRU were used as tests for cytotoxicity. The pulp tissue levels of MEL, inducible NO synthase (iNOS) and superoxide dismutase (SOD) activity, as well as iNOS, histone acetyltransferase p300 (p300) and SOD activity levels in hDPCs incubated with MEL (0.1 and 1.0mmolL(-1)) under normoglycaemia and hyperglycaemia were measured by enzyme-linked immunosorbent assay. Comparisons between the two groups were made by unpaired t-tests or Mann-Whitney test whilst the chi-square test was used for dichotomous variables. To compare more groups, the Kruskal-Wallis test with Dunn's multiple comparison was used, whilst Spearman correlation was used to assess association between two variables. ResultsMelatonin was decreased (124.3021.6 vs. 240.0 +/- 19.1 pgmL(-1), P lt 0.01), whilst iNOS levels increased (0.92 +/- 0.08 vs. 0.32 +/- 0.09ngmL(-1), P lt 0.01) in hDP from T2D compared to nondiabetic participants. In hDPCs, MEL (0.1 and 1.0mmolL(-1)) had no cytotoxicity. Incubation with 1.0mmolL(-1) of MEL (24h) decreased hyperglycaemia-induced increases of iNOS (0.34 +/- 0.01ngmL(-1) vs. 0.40 +/- 0.01ngmL(-1), P lt 0.01) and p300 (11.59 +/- 0.58ngmL(-1) vs. 16.12 +/- 0.39ngmL(-1), P lt 0.01), and also, increased SOD activity (87.11 +/- 3.10% vs. 68.56 +/- 3.77%, P lt 0.01) to the levels comparable to the normoglycaemic; iNOS and p300 protein expression levels showed strong positive correlation under hyperglycaemia (Spearman r=0.8242, P lt 0.001). ConclusionType 2 diabetic participants had decreased MEL in hDP. At pharmacological concentrations, MEL is not cytotoxic for hDPCs and normalizes iNOS and SOD activity levels in hyperglyceamic hDPCs suggesting its antioxidant and protective effects in human dental pulp tissue under hyperglycaemia.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions",
volume = "51",
number = "10",
pages = "1149-1158",
doi = "10.1111/iej.12934"
}

Milosavljević, A., Đukić, L., Toljić, B., Milašin, J., Dželetović, B., Brković, B.,& Roganović, J.. (2018). Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions. in International Endodontic Journal
Wiley, Hoboken., 51(10), 1149-1158.
https://doi.org/10.1111/iej.12934

Milosavljević A, Đukić L, Toljić B, Milašin J, Dželetović B, Brković B, Roganović J. Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions. in International Endodontic Journal. 2018;51(10):1149-1158.
doi:10.1111/iej.12934 .

Milosavljević, A., Đukić, Ljiljana, Toljić, Boško, Milašin, Jelena, Dželetović, Bojan, Brković, Božidar, Roganović, Jelena, "Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions" in International Endodontic Journal, 51, no. 10 (2018):1149-1158,
https://doi.org/10.1111/iej.12934 . .

TY  - JOUR
AU  - Radović, Nikola
AU  - Nikolić-Jakoba, Nataša
AU  - Petrović, Nina
AU  - Milosavljević, Aleksandra
AU  - Brković, Božidar
AU  - Roganović, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2519
AB  - AimRecent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively). Material and methodsmiR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically. ResultsThe upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender. ConclusionsmiR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients.
PB  - Wiley, Hoboken
T2  - Journal of Clinical Periodontology
T1  - MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients
VL  - 45
IS  - 6
SP  - 663
EP  - 671
DO  - 10.1111/jcpe.12888
ER  - 

@article{
author = "Radović, Nikola and Nikolić-Jakoba, Nataša and Petrović, Nina and Milosavljević, Aleksandra and Brković, Božidar and Roganović, Jelena",
year = "2018",
abstract = "AimRecent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively). Material and methodsmiR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically. ResultsThe upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender. ConclusionsmiR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients.",
publisher = "Wiley, Hoboken",
journal = "Journal of Clinical Periodontology",
title = "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients",
volume = "45",
number = "6",
pages = "663-671",
doi = "10.1111/jcpe.12888"
}

Radović, N., Nikolić-Jakoba, N., Petrović, N., Milosavljević, A., Brković, B.,& Roganović, J.. (2018). MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. in Journal of Clinical Periodontology
Wiley, Hoboken., 45(6), 663-671.
https://doi.org/10.1111/jcpe.12888

Radović N, Nikolić-Jakoba N, Petrović N, Milosavljević A, Brković B, Roganović J. MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. in Journal of Clinical Periodontology. 2018;45(6):663-671.
doi:10.1111/jcpe.12888 .

Radović, Nikola, Nikolić-Jakoba, Nataša, Petrović, Nina, Milosavljević, Aleksandra, Brković, Božidar, Roganović, Jelena, "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients" in Journal of Clinical Periodontology, 45, no. 6 (2018):663-671,
https://doi.org/10.1111/jcpe.12888 . .

TY  - JOUR
AU  - Radović, Nikola
AU  - Nikolić-Jakoba, Nataša
AU  - Petrović, Nina
AU  - Milosavljević, Aleksandra
AU  - Brković, Božidar
AU  - Roganović, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2282
AB  - AimRecent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively). Material and methodsmiR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically. ResultsThe upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender. ConclusionsmiR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients.
PB  - Wiley, Hoboken
T2  - Journal of Clinical Periodontology
T1  - MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients
VL  - 45
IS  - 6
SP  - 663
EP  - 671
DO  - 10.1111/jcpe.12888
ER  - 

@article{
author = "Radović, Nikola and Nikolić-Jakoba, Nataša and Petrović, Nina and Milosavljević, Aleksandra and Brković, Božidar and Roganović, Jelena",
year = "2018",
abstract = "AimRecent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively). Material and methodsmiR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically. ResultsThe upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender. ConclusionsmiR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients.",
publisher = "Wiley, Hoboken",
journal = "Journal of Clinical Periodontology",
title = "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients",
volume = "45",
number = "6",
pages = "663-671",
doi = "10.1111/jcpe.12888"
}

Radović, N., Nikolić-Jakoba, N., Petrović, N., Milosavljević, A., Brković, B.,& Roganović, J.. (2018). MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. in Journal of Clinical Periodontology
Wiley, Hoboken., 45(6), 663-671.
https://doi.org/10.1111/jcpe.12888

Radović N, Nikolić-Jakoba N, Petrović N, Milosavljević A, Brković B, Roganović J. MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. in Journal of Clinical Periodontology. 2018;45(6):663-671.
doi:10.1111/jcpe.12888 .

Radović, Nikola, Nikolić-Jakoba, Nataša, Petrović, Nina, Milosavljević, Aleksandra, Brković, Božidar, Roganović, Jelena, "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients" in Journal of Clinical Periodontology, 45, no. 6 (2018):663-671,
https://doi.org/10.1111/jcpe.12888 . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Davidović, Radoslav
AU  - Jovanović-Cupić, Snezana
AU  - Krajnović, Milena
AU  - Lukić, Silvana
AU  - Petrović, Milan
AU  - Roganović, Jelena
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2120
AB  - Breast cancer (BC) is a heterogeneous group of diseases that still represents a major cause of death in the female population. MicroRNAs (miRNAs, miRs), such as miR-221 and miR-222, have been shown to be involved in BC pathology by acting via its target genes such as tissue inhibitor of metalloproteinase 3 (TIMP3). The main goals of this study were to find differences in miR-221/222 levels of expression in BC groups based on invasiveness, and to investigate the association with estrogen receptor (ER), TIMP3 messenger RNA (mRNA) levels, and clinicopathological characteristics of patients and tumors. In this study, we measured levels of miR-221/222 in 63 breast tissue samples by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) using TaqMan(A (R)) technology and immunohistochemistry. miR-221/222 levels varied significantly across groups based on invasiveness (P  lt  0.001). In in situ tumors, miR-221 and miR-222 were negatively associated with ER (P = 0.001, r = -0.714, and P = 0.013, r = -0.585, respectively). In invasive breast carcinomas associated with non-invasive tumors, miR-222 was inversely associated with ER (P = 0.039, r = -0.620). Pure invasive BCs showed a positive correlation of miR-221 and miR-222 with TIMP3 mRNA levels (P = 0.008, r = 0.508, and P = 0.010, r = 0.497, respectively). An increase in miR-221/222 might be an important event for in situ carcinoma formation, and miR-221/222 may be important molecules that highlight potential differences between invasive breast carcinomas associated with non-invasive and pure invasive BCs.
PB  - Adis Int Ltd, Northcote
T2  - Molecular Diagnosis & Therapy
T1  - Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels
VL  - 20
IS  - 6
SP  - 603
EP  - 615
DO  - 10.1007/s40291-016-0230-3
ER  - 

@article{
author = "Petrović, Nina and Davidović, Radoslav and Jovanović-Cupić, Snezana and Krajnović, Milena and Lukić, Silvana and Petrović, Milan and Roganović, Jelena",
year = "2016",
abstract = "Breast cancer (BC) is a heterogeneous group of diseases that still represents a major cause of death in the female population. MicroRNAs (miRNAs, miRs), such as miR-221 and miR-222, have been shown to be involved in BC pathology by acting via its target genes such as tissue inhibitor of metalloproteinase 3 (TIMP3). The main goals of this study were to find differences in miR-221/222 levels of expression in BC groups based on invasiveness, and to investigate the association with estrogen receptor (ER), TIMP3 messenger RNA (mRNA) levels, and clinicopathological characteristics of patients and tumors. In this study, we measured levels of miR-221/222 in 63 breast tissue samples by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) using TaqMan(A (R)) technology and immunohistochemistry. miR-221/222 levels varied significantly across groups based on invasiveness (P  lt  0.001). In in situ tumors, miR-221 and miR-222 were negatively associated with ER (P = 0.001, r = -0.714, and P = 0.013, r = -0.585, respectively). In invasive breast carcinomas associated with non-invasive tumors, miR-222 was inversely associated with ER (P = 0.039, r = -0.620). Pure invasive BCs showed a positive correlation of miR-221 and miR-222 with TIMP3 mRNA levels (P = 0.008, r = 0.508, and P = 0.010, r = 0.497, respectively). An increase in miR-221/222 might be an important event for in situ carcinoma formation, and miR-221/222 may be important molecules that highlight potential differences between invasive breast carcinomas associated with non-invasive and pure invasive BCs.",
publisher = "Adis Int Ltd, Northcote",
journal = "Molecular Diagnosis & Therapy",
title = "Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels",
volume = "20",
number = "6",
pages = "603-615",
doi = "10.1007/s40291-016-0230-3"
}

Petrović, N., Davidović, R., Jovanović-Cupić, S., Krajnović, M., Lukić, S., Petrović, M.,& Roganović, J.. (2016). Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels. in Molecular Diagnosis & Therapy
Adis Int Ltd, Northcote., 20(6), 603-615.
https://doi.org/10.1007/s40291-016-0230-3

Petrović N, Davidović R, Jovanović-Cupić S, Krajnović M, Lukić S, Petrović M, Roganović J. Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels. in Molecular Diagnosis & Therapy. 2016;20(6):603-615.
doi:10.1007/s40291-016-0230-3 .

Petrović, Nina, Davidović, Radoslav, Jovanović-Cupić, Snezana, Krajnović, Milena, Lukić, Silvana, Petrović, Milan, Roganović, Jelena, "Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels" in Molecular Diagnosis & Therapy, 20, no. 6 (2016):603-615,
https://doi.org/10.1007/s40291-016-0230-3 . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Jovanović-Cupić, Snezana
AU  - Brajušković, Goran
AU  - Lukić, Silvana
AU  - Roganović, Jelena
AU  - Krajnović, Milena
AU  - Mandusić, Vesna
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1991
AB  - Invasive ductal carcinomas with a non-invasive component (IDC-DCIS) are classified as a group of invasive breast carcinomas, together with pure invasive ductal carcinomas of the breast (IDC). MicroRNA-21 (miR-21) has been characterized as a factor of breast cancer invasiveness, however the difference in miR-21 expression levels between IDC-DCIS and pure IDC tumors and the correlations with standard diagnostic and prognostic parameters inside the IDC-DCIS group are unknown. Our aim was to determine if miR-21 had the ability to distinguish these two invasive breast cancer groups. Levels of miR-21 expression were measured by a stem-loop quantitative Real-Time PCR (RT-qPCR) method. Expression levels of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her-2) and proliferative index Ki-67 were evaluated by immunohistochemistry. IDC-DCIS tumors had significantly lower levels of miR-21 expression in grade 2 (P=0.003, Mann-Whitney U test), ER positive (P=0.025, Mann-Whitney U test) and PR positive tumors (P=0.024, Mann-Whitney U test) than pure IDCs. miR-21 levels showed a different pattern of expression in IDC-DCIS compared to IDC tumors, which is based on the difference in miR-21 expression between Her-2 negative and Her-2 positive IDC-DCIS tumors (P=0.030, Mann-Whitney U test) and high negative correlation of miR-21 levels with PR levels (rho=-0.886, P=0.006, Spearman correlation). According to our results, IDC-DCIS breast carcinomas act in a different manner in pure IDC tumors with regard to the relations between miR-21 expression levels and the standard diagnostic and prognostic parameters, such as Her-2 status, ER and PR status and protein levels.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Micro RNA-21 expression levels in invasive breast carcinoma with a non-invasive component
VL  - 67
IS  - 4
SP  - 1285
EP  - 1295
DO  - 10.2298/ABS150327105P
ER  - 

@article{
author = "Petrović, Nina and Jovanović-Cupić, Snezana and Brajušković, Goran and Lukić, Silvana and Roganović, Jelena and Krajnović, Milena and Mandusić, Vesna",
year = "2015",
abstract = "Invasive ductal carcinomas with a non-invasive component (IDC-DCIS) are classified as a group of invasive breast carcinomas, together with pure invasive ductal carcinomas of the breast (IDC). MicroRNA-21 (miR-21) has been characterized as a factor of breast cancer invasiveness, however the difference in miR-21 expression levels between IDC-DCIS and pure IDC tumors and the correlations with standard diagnostic and prognostic parameters inside the IDC-DCIS group are unknown. Our aim was to determine if miR-21 had the ability to distinguish these two invasive breast cancer groups. Levels of miR-21 expression were measured by a stem-loop quantitative Real-Time PCR (RT-qPCR) method. Expression levels of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her-2) and proliferative index Ki-67 were evaluated by immunohistochemistry. IDC-DCIS tumors had significantly lower levels of miR-21 expression in grade 2 (P=0.003, Mann-Whitney U test), ER positive (P=0.025, Mann-Whitney U test) and PR positive tumors (P=0.024, Mann-Whitney U test) than pure IDCs. miR-21 levels showed a different pattern of expression in IDC-DCIS compared to IDC tumors, which is based on the difference in miR-21 expression between Her-2 negative and Her-2 positive IDC-DCIS tumors (P=0.030, Mann-Whitney U test) and high negative correlation of miR-21 levels with PR levels (rho=-0.886, P=0.006, Spearman correlation). According to our results, IDC-DCIS breast carcinomas act in a different manner in pure IDC tumors with regard to the relations between miR-21 expression levels and the standard diagnostic and prognostic parameters, such as Her-2 status, ER and PR status and protein levels.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Micro RNA-21 expression levels in invasive breast carcinoma with a non-invasive component",
volume = "67",
number = "4",
pages = "1285-1295",
doi = "10.2298/ABS150327105P"
}

Petrović, N., Jovanović-Cupić, S., Brajušković, G., Lukić, S., Roganović, J., Krajnović, M.,& Mandusić, V.. (2015). Micro RNA-21 expression levels in invasive breast carcinoma with a non-invasive component. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 67(4), 1285-1295.
https://doi.org/10.2298/ABS150327105P

Petrović N, Jovanović-Cupić S, Brajušković G, Lukić S, Roganović J, Krajnović M, Mandusić V. Micro RNA-21 expression levels in invasive breast carcinoma with a non-invasive component. in Archives of Biological Sciences. 2015;67(4):1285-1295.
doi:10.2298/ABS150327105P .

Petrović, Nina, Jovanović-Cupić, Snezana, Brajušković, Goran, Lukić, Silvana, Roganović, Jelena, Krajnović, Milena, Mandusić, Vesna, "Micro RNA-21 expression levels in invasive breast carcinoma with a non-invasive component" in Archives of Biological Sciences, 67, no. 4 (2015):1285-1295,
https://doi.org/10.2298/ABS150327105P . .

TY  - JOUR
AU  - Roganović, Jelena
AU  - Đukić, Ljiljana
AU  - Kršljak, Elena
AU  - Tanić, Nasta
AU  - Stojić, Dragica
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1963
AB  - ObjectivesThe influence of experimental diabetes (alloxan, 100mgkg(-1)) was studied on rabbit parotid gland function. Material and MethodsCarbachol-induced parotid secretion in vivo, and in vitro quantification of inducible nitric oxide synthase (iNOS) mRNA expression, by real-time RT-PCR, and activity of superoxide dismutase (SOD) and total antioxidant capacity (TAC) in commercial colorimetric assays were measured in parotid glands of non-diabetic and diabetic rabbits. ResultsCarbachol-induced dose-dependent increase in parotid secretion significantly reduced in diabetic rabbits. Functional studies in the presence of muscarinic receptor and nitric oxide synthase (NOS) antagonists revealed that in M-3 receptor-mediated carbachol secretion, nitric oxide, deriving mainly from neuronal NOS (nNOS) in control, and iNOS in diabetic rabbits, was involved. Also, upregulation of iNOS mRNA expression and enhanced SOD activity and TAC were detected in diabetic glands. ConclusionsOur data suggest that decreased M-3 receptor-mediated parotid secretion in diabetic rabbits appears to be due to alterations in NO signaling, mainly due to iNOS induction, accompanied by elevated antioxidant response.
PB  - Wiley, Hoboken
T2  - Oral Diseases
T1  - Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits
VL  - 21
IS  - 5
SP  - 634
EP  - 640
DO  - 10.1111/odi.12327
ER  - 

@article{
author = "Roganović, Jelena and Đukić, Ljiljana and Kršljak, Elena and Tanić, Nasta and Stojić, Dragica",
year = "2015",
abstract = "ObjectivesThe influence of experimental diabetes (alloxan, 100mgkg(-1)) was studied on rabbit parotid gland function. Material and MethodsCarbachol-induced parotid secretion in vivo, and in vitro quantification of inducible nitric oxide synthase (iNOS) mRNA expression, by real-time RT-PCR, and activity of superoxide dismutase (SOD) and total antioxidant capacity (TAC) in commercial colorimetric assays were measured in parotid glands of non-diabetic and diabetic rabbits. ResultsCarbachol-induced dose-dependent increase in parotid secretion significantly reduced in diabetic rabbits. Functional studies in the presence of muscarinic receptor and nitric oxide synthase (NOS) antagonists revealed that in M-3 receptor-mediated carbachol secretion, nitric oxide, deriving mainly from neuronal NOS (nNOS) in control, and iNOS in diabetic rabbits, was involved. Also, upregulation of iNOS mRNA expression and enhanced SOD activity and TAC were detected in diabetic glands. ConclusionsOur data suggest that decreased M-3 receptor-mediated parotid secretion in diabetic rabbits appears to be due to alterations in NO signaling, mainly due to iNOS induction, accompanied by elevated antioxidant response.",
publisher = "Wiley, Hoboken",
journal = "Oral Diseases",
title = "Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits",
volume = "21",
number = "5",
pages = "634-640",
doi = "10.1111/odi.12327"
}

Roganović, J., Đukić, L., Kršljak, E., Tanić, N.,& Stojić, D.. (2015). Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits. in Oral Diseases
Wiley, Hoboken., 21(5), 634-640.
https://doi.org/10.1111/odi.12327

Roganović J, Đukić L, Kršljak E, Tanić N, Stojić D. Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits. in Oral Diseases. 2015;21(5):634-640.
doi:10.1111/odi.12327 .

Roganović, Jelena, Đukić, Ljiljana, Kršljak, Elena, Tanić, Nasta, Stojić, Dragica, "Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits" in Oral Diseases, 21, no. 5 (2015):634-640,
https://doi.org/10.1111/odi.12327 . .

TY  - JOUR
AU  - Đukić, Ljiljana
AU  - Roganović, Jelena
AU  - Brajović, Milan
AU  - Bokonjić, Dejan
AU  - Stojić, Dragica
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1961
AB  - ObjectiveThe present cross-sectional study aimed to determine the effect of first-line anti-hypertensive drugs (enalapril, metoprolol, and combinations of enalapril with metoprolol and/or hydrochlorothiazide) on salivary gland function and salivary total antioxidant capacity (TAC) in hypertensive patients with/without diabetes mellitus (DM) type 2. Materials and MethodsSalivary gland function was measured as xerostomia (interview) and unstimulated whole saliva flow rate (UWSFR) in 447 subjects (387 hypertensive and 60 healthy). Salivary TAC was evaluated by spectrophotometric assay. ResultsEnalapril is not xerogenic, while metoprolol and drug combinations are. In the presence of DM type 2, all drugs, except metoprolol, had pronounced xerogenic effect. Binary logistic regression analysis found enalapril to be significantly associated with decreased risk of xerogenic effect development, while DM type 2 with increased risk. In the presence of enalapril in hypertensive patients with/without DM type 2 salivary TAC was similar to that in healthy subjects, while for metoprolol was reduced. ConclusionsEnalapril is not xerogenic but is antioxidant, which moderately reduces the risk of xerogenic effect development even in the presence of DM type 2. However, metoprolol and drug combinations exhibit xerogenic effect. In DM type 2, xerogenic effect of all drugs was pronounced except of metoprolol.
PB  - Wiley, Hoboken
T2  - Oral Diseases
T1  - The effects of anti-hypertensives and type 2 diabetes on salivary flow and total antioxidant capacity
VL  - 21
IS  - 5
SP  - 619
EP  - 625
DO  - 10.1111/odi.12325
ER  - 

@article{
author = "Đukić, Ljiljana and Roganović, Jelena and Brajović, Milan and Bokonjić, Dejan and Stojić, Dragica",
year = "2015",
abstract = "ObjectiveThe present cross-sectional study aimed to determine the effect of first-line anti-hypertensive drugs (enalapril, metoprolol, and combinations of enalapril with metoprolol and/or hydrochlorothiazide) on salivary gland function and salivary total antioxidant capacity (TAC) in hypertensive patients with/without diabetes mellitus (DM) type 2. Materials and MethodsSalivary gland function was measured as xerostomia (interview) and unstimulated whole saliva flow rate (UWSFR) in 447 subjects (387 hypertensive and 60 healthy). Salivary TAC was evaluated by spectrophotometric assay. ResultsEnalapril is not xerogenic, while metoprolol and drug combinations are. In the presence of DM type 2, all drugs, except metoprolol, had pronounced xerogenic effect. Binary logistic regression analysis found enalapril to be significantly associated with decreased risk of xerogenic effect development, while DM type 2 with increased risk. In the presence of enalapril in hypertensive patients with/without DM type 2 salivary TAC was similar to that in healthy subjects, while for metoprolol was reduced. ConclusionsEnalapril is not xerogenic but is antioxidant, which moderately reduces the risk of xerogenic effect development even in the presence of DM type 2. However, metoprolol and drug combinations exhibit xerogenic effect. In DM type 2, xerogenic effect of all drugs was pronounced except of metoprolol.",
publisher = "Wiley, Hoboken",
journal = "Oral Diseases",
title = "The effects of anti-hypertensives and type 2 diabetes on salivary flow and total antioxidant capacity",
volume = "21",
number = "5",
pages = "619-625",
doi = "10.1111/odi.12325"
}

Đukić, L., Roganović, J., Brajović, M., Bokonjić, D.,& Stojić, D.. (2015). The effects of anti-hypertensives and type 2 diabetes on salivary flow and total antioxidant capacity. in Oral Diseases
Wiley, Hoboken., 21(5), 619-625.
https://doi.org/10.1111/odi.12325

Đukić L, Roganović J, Brajović M, Bokonjić D, Stojić D. The effects of anti-hypertensives and type 2 diabetes on salivary flow and total antioxidant capacity. in Oral Diseases. 2015;21(5):619-625.
doi:10.1111/odi.12325 .

Đukić, Ljiljana, Roganović, Jelena, Brajović, Milan, Bokonjić, Dejan, Stojić, Dragica, "The effects of anti-hypertensives and type 2 diabetes on salivary flow and total antioxidant capacity" in Oral Diseases, 21, no. 5 (2015):619-625,
https://doi.org/10.1111/odi.12325 . .

TY  - JOUR
AU  - Radović, Katarina
AU  - Ilić, Jugoslav
AU  - Roganović, Jelena
AU  - Stojić, Dragica
AU  - Brković, Božidar
AU  - Pudar, Georgina
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1939
AB  - Statement of problem. Diabetes mellitus type 2 is associated with a variety of oral mucosal changes, including an altered level of salivary vascular endothelial growth factor. However, the authors identified no studies concerning denture stomatitis and salivary vascular endothelial growth factor in immediate denture wearers with diabetes mellitus type 2. Purpose. The purpose of this study was to investigate the incidence of and risk factors for denture stomatitis in immediate complete denture wearers with and without diabetes mellitus type 2 and to investigate the relationship between levels of salivary vascular endothelial growth factor and clinical forms of denture stomatitis in both groups. Material and methods. Individuals without diabetes (n=42) or with diabetes mellitus type 2 (n=36) who were candidates for complete immediate dentures were included in the study. After 1 year of wearing relined immediate dentures, participants were evaluated for denture stomatitis and potential contributing factors. Salivary vascular endothelial growth factor levels were measured with enzyme-linked immunosorbent assays. Data were analyzed with the chi(2) test or the Student t test where appropriate, as well as with binary logistic regression analysis. Results. The incidence of denture stomatitis was 61% in participants with diabetes mellitus type 2 and 38% in those without diabetes. Low denture stability and diabetes mellitus type 2 were risk factors for denture stomatitis. Salivary vascular endothelial growth factor concentrations at the beginning of the study were 557.6 +/- 94.7 pg/mL in participants with diabetes mellitus type 2 and 103.5 +/- 21.6 pg/mL in those without diabetes. In Newton Type I and Newton Type II denture stomatitis, vascular endothelial growth factor levels were 460.9 +/- 55.4 pg/mL and 1445.2 +/- 422.1 pg/mL in individuals with diabetes and 73.2 +/- 10.0 pg/mL and 306.5 +/- 22.6 pg/mL in those without diabetes. Conclusions. Perceived denture stability and diabetes mellitus type 2 are independent risk factors for the occurrence of denture stomatitis. Altered salivary vascular endothelial growth factor levels and denture stomatitis are more prevalent in denture wearers with diabetes mellitus type 2.
PB  - Mosby-Elsevier, New York
T2  - Journal of Prosthetic Dentistry
T1  - Denture stomatitis and salivary vascular endothelial growth factor in immediate complete denture wearers with type 2 diabetes
VL  - 111
IS  - 5
SP  - 373
EP  - 379
DO  - 10.1016/j.prosdent.2013.07.019
ER  - 

@article{
author = "Radović, Katarina and Ilić, Jugoslav and Roganović, Jelena and Stojić, Dragica and Brković, Božidar and Pudar, Georgina",
year = "2014",
abstract = "Statement of problem. Diabetes mellitus type 2 is associated with a variety of oral mucosal changes, including an altered level of salivary vascular endothelial growth factor. However, the authors identified no studies concerning denture stomatitis and salivary vascular endothelial growth factor in immediate denture wearers with diabetes mellitus type 2. Purpose. The purpose of this study was to investigate the incidence of and risk factors for denture stomatitis in immediate complete denture wearers with and without diabetes mellitus type 2 and to investigate the relationship between levels of salivary vascular endothelial growth factor and clinical forms of denture stomatitis in both groups. Material and methods. Individuals without diabetes (n=42) or with diabetes mellitus type 2 (n=36) who were candidates for complete immediate dentures were included in the study. After 1 year of wearing relined immediate dentures, participants were evaluated for denture stomatitis and potential contributing factors. Salivary vascular endothelial growth factor levels were measured with enzyme-linked immunosorbent assays. Data were analyzed with the chi(2) test or the Student t test where appropriate, as well as with binary logistic regression analysis. Results. The incidence of denture stomatitis was 61% in participants with diabetes mellitus type 2 and 38% in those without diabetes. Low denture stability and diabetes mellitus type 2 were risk factors for denture stomatitis. Salivary vascular endothelial growth factor concentrations at the beginning of the study were 557.6 +/- 94.7 pg/mL in participants with diabetes mellitus type 2 and 103.5 +/- 21.6 pg/mL in those without diabetes. In Newton Type I and Newton Type II denture stomatitis, vascular endothelial growth factor levels were 460.9 +/- 55.4 pg/mL and 1445.2 +/- 422.1 pg/mL in individuals with diabetes and 73.2 +/- 10.0 pg/mL and 306.5 +/- 22.6 pg/mL in those without diabetes. Conclusions. Perceived denture stability and diabetes mellitus type 2 are independent risk factors for the occurrence of denture stomatitis. Altered salivary vascular endothelial growth factor levels and denture stomatitis are more prevalent in denture wearers with diabetes mellitus type 2.",
publisher = "Mosby-Elsevier, New York",
journal = "Journal of Prosthetic Dentistry",
title = "Denture stomatitis and salivary vascular endothelial growth factor in immediate complete denture wearers with type 2 diabetes",
volume = "111",
number = "5",
pages = "373-379",
doi = "10.1016/j.prosdent.2013.07.019"
}

Radović, K., Ilić, J., Roganović, J., Stojić, D., Brković, B.,& Pudar, G.. (2014). Denture stomatitis and salivary vascular endothelial growth factor in immediate complete denture wearers with type 2 diabetes. in Journal of Prosthetic Dentistry
Mosby-Elsevier, New York., 111(5), 373-379.
https://doi.org/10.1016/j.prosdent.2013.07.019

Radović K, Ilić J, Roganović J, Stojić D, Brković B, Pudar G. Denture stomatitis and salivary vascular endothelial growth factor in immediate complete denture wearers with type 2 diabetes. in Journal of Prosthetic Dentistry. 2014;111(5):373-379.
doi:10.1016/j.prosdent.2013.07.019 .

Radović, Katarina, Ilić, Jugoslav, Roganović, Jelena, Stojić, Dragica, Brković, Božidar, Pudar, Georgina, "Denture stomatitis and salivary vascular endothelial growth factor in immediate complete denture wearers with type 2 diabetes" in Journal of Prosthetic Dentistry, 111, no. 5 (2014):373-379,
https://doi.org/10.1016/j.prosdent.2013.07.019 . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Mandusić, Vesna
AU  - Dimitrijević, Bogomir
AU  - Roganović, Jelena
AU  - Lukić, Silvana
AU  - Todorović, Lidija
AU  - Stanojević, Boban
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1930
AB  - MicroRNAs play essential role in breast carcinoma progression and invasion. Our principal goals were to assess clinicopathological and prognostic correlations of microRNA-21 (miR-21) expression levels in a group of 39 Serbian breast cancer patients with invasive lobular (ILC), ductal (IDC), or mixed (ILC-IDC) breast carcinomas and in order to discover the role of miR-21 in potential novel form of stratification of the patients with different estrogen receptor (ER) and progesterone receptor (PR) status. MiR-21 expression levels were measured by stem-loop real-time RT-PCR using TaqMan technology. ER, PR, human epidermal growth factor 2 receptor (Her-2), and proliferative index (Ki-67) were evaluated by immunohistochemistry. MiR-21 levels do not vary among ILC, IDC, and ILC-IDC subgroups. MiR-21 expression levels varied significantly in the age, tumor size, Ki-67, and different grade (p = 0.030, p = 0.036, p = 0.027 and p = 0.032, respectively) subgroups. ER? and PR? showed higher miR-21 levels than their negative receptor status paired groups ER-and PR-with p = 0.012 and p = 0.018, respectively. MiR-21 positively correlated with ER and PR status (p = 0.018, rho = 0.379 and p = 0.034, rho = 0.345, respectively). Our findings suggest that miR-21 emulates transitional form of expression and that the levels of expression might be useful for stratification of the patients with different receptor status with the purpose to seek for new therapy approaches especially for the patients with the lack of response to conventional endocrine therapy.
PB  - Humana Press Inc, Totowa
T2  - Medical Oncology
T1  - Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia
VL  - 31
IS  - 6
DO  - 10.1007/s12032-014-0977-5
ER  - 

@article{
author = "Petrović, Nina and Mandusić, Vesna and Dimitrijević, Bogomir and Roganović, Jelena and Lukić, Silvana and Todorović, Lidija and Stanojević, Boban",
year = "2014",
abstract = "MicroRNAs play essential role in breast carcinoma progression and invasion. Our principal goals were to assess clinicopathological and prognostic correlations of microRNA-21 (miR-21) expression levels in a group of 39 Serbian breast cancer patients with invasive lobular (ILC), ductal (IDC), or mixed (ILC-IDC) breast carcinomas and in order to discover the role of miR-21 in potential novel form of stratification of the patients with different estrogen receptor (ER) and progesterone receptor (PR) status. MiR-21 expression levels were measured by stem-loop real-time RT-PCR using TaqMan technology. ER, PR, human epidermal growth factor 2 receptor (Her-2), and proliferative index (Ki-67) were evaluated by immunohistochemistry. MiR-21 levels do not vary among ILC, IDC, and ILC-IDC subgroups. MiR-21 expression levels varied significantly in the age, tumor size, Ki-67, and different grade (p = 0.030, p = 0.036, p = 0.027 and p = 0.032, respectively) subgroups. ER? and PR? showed higher miR-21 levels than their negative receptor status paired groups ER-and PR-with p = 0.012 and p = 0.018, respectively. MiR-21 positively correlated with ER and PR status (p = 0.018, rho = 0.379 and p = 0.034, rho = 0.345, respectively). Our findings suggest that miR-21 emulates transitional form of expression and that the levels of expression might be useful for stratification of the patients with different receptor status with the purpose to seek for new therapy approaches especially for the patients with the lack of response to conventional endocrine therapy.",
publisher = "Humana Press Inc, Totowa",
journal = "Medical Oncology",
title = "Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia",
volume = "31",
number = "6",
doi = "10.1007/s12032-014-0977-5"
}

Petrović, N., Mandusić, V., Dimitrijević, B., Roganović, J., Lukić, S., Todorović, L.,& Stanojević, B.. (2014). Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia. in Medical Oncology
Humana Press Inc, Totowa., 31(6).
https://doi.org/10.1007/s12032-014-0977-5

Petrović N, Mandusić V, Dimitrijević B, Roganović J, Lukić S, Todorović L, Stanojević B. Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia. in Medical Oncology. 2014;31(6).
doi:10.1007/s12032-014-0977-5 .

Petrović, Nina, Mandusić, Vesna, Dimitrijević, Bogomir, Roganović, Jelena, Lukić, Silvana, Todorović, Lidija, Stanojević, Boban, "Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia" in Medical Oncology, 31, no. 6 (2014),
https://doi.org/10.1007/s12032-014-0977-5 . .

TY  - JOUR
AU  - Roganović, Jelena
AU  - Petrović, Nina
AU  - Đukić, Ljiljana
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1902
AB  - Background/Aim. Atherosclerotic-occlusive changes could be observed in orofacial branches of the external carotid artery. Atherosclerosis-induced ischemia caused alteration in production and release of endothelial factors. The aim of this study was to investigate the influence of carotid artery occlusion (10, 30 and 60 min) on vascular effects of norepinephrine (NOR) and neuropeptide Y (NPY) in the isolated glandular branch of the rabbit facial artery, the main feeding artery for the submandibular gland. Method. Changes in isometric tension were recorded in organ bath studies with arterial rings, before and after carotid artery occlusion. Results. Concentrationdependent vasocontractile effect of NOR was significantly augmented after 30 and 60 min of carotid occlusion, but only in the rings with intact endothelium. Given alone, NPY showed no effect in isolated glandular branch of the rabbit facial artery, but enhanced NOR vasoconstriction in all the investigated rings. NOR vasocontractile effect enhancement in the presence of NPY was attenuated after 30 and 60 min of carotid occlusion. Also, enhancement of NOR vasoconstriction by NPY was significantly higher in endothelium-intact rings compared to endotheliumdenuded rings obtained after 30 and 60 min of carotid occlusion. Conclusion. The present investigation provides results of increased vasocontractile effect of NOR and decreased enhancing effect of NPY on NOR vasoconstriction in the rabbit facial artery after carotid occlusion that is related to altered endothelium function.
AB  - Uvod/Cilj. Pokazano je da se okluzivne promene aterosklerotične prirode mogu opaziti na orofacijalnim granama spoljne karotidne arterije. Ishemija izazvana aterosklerozom, dovodi do poremećaja stvaranja i oslobađanja faktora poreklom iz endotela. Cilj ovog istraživanja bio je da se ispita uticaj okluzije karotidne arterije (10, 30 i 60 min) na vazokontraktilne efekte norepinefrina (NOR) i neuropeptida Y (NPY) na izolovanoj žlezdanoj grani facijalne arterije kunića, glavne dovodne arterije za submandibularnu žlezdu. Metode. U kupatilu za izolovane krvne sudove ispitivane su izometrijske promene tonusa arterijskih preparata, pre i posle okluzije karotidne arterije. Rezultati. Koncentracijski-zavisan vazokontraktilni efekat NOR bio je značajno veći posle 30 i 60 min karotidne okluzije, ali samo na preparatima sa očuvanim endotelom. Primenjen u rastućim koncentracijama, NPY nije imao efekta na tonus izolovane žlezdane grane facijalne aterije kunića, ali je povećao vazokontraktilni efekat NOR na svim ispitivanim preparatima. Povećanje vazokontraktilnog efekta NOR u prisustvu NPY bilo je značajno manje posle 30 i 60 min okluzije. Takođe, efekat NPY posle karotidne okluzije bio je značajno veći na preparatima sa očuvanim endotelom u odnosu na preparate sa uklonjenim endotelom. Zaključak. Ovo istraživanje pokazalo je povećanje vazokontraktilnog efekta NOR i smanjenje potencirajućeg efekta NPY na vazokonstrikciju izazvanu NOR na facijalnoj arteriji kunića posle okluzije karotidne arterije koje su povezane sa promenjenom funkcijom endotela.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Effect of neuropeptide Y on norepinephrine-induced constriction in the rabbit facial artery after carotid artery occlusion
T1  - Efekat neuropeptida Y na konstrikciju facijalne arterije kunića izazvane norepinefrinom posle okluzije karotidne arterije
VL  - 71
IS  - 6
SP  - 571
EP  - 575
DO  - 10.2298/VSP1406571R
ER  - 

@article{
author = "Roganović, Jelena and Petrović, Nina and Đukić, Ljiljana",
year = "2014",
abstract = "Background/Aim. Atherosclerotic-occlusive changes could be observed in orofacial branches of the external carotid artery. Atherosclerosis-induced ischemia caused alteration in production and release of endothelial factors. The aim of this study was to investigate the influence of carotid artery occlusion (10, 30 and 60 min) on vascular effects of norepinephrine (NOR) and neuropeptide Y (NPY) in the isolated glandular branch of the rabbit facial artery, the main feeding artery for the submandibular gland. Method. Changes in isometric tension were recorded in organ bath studies with arterial rings, before and after carotid artery occlusion. Results. Concentrationdependent vasocontractile effect of NOR was significantly augmented after 30 and 60 min of carotid occlusion, but only in the rings with intact endothelium. Given alone, NPY showed no effect in isolated glandular branch of the rabbit facial artery, but enhanced NOR vasoconstriction in all the investigated rings. NOR vasocontractile effect enhancement in the presence of NPY was attenuated after 30 and 60 min of carotid occlusion. Also, enhancement of NOR vasoconstriction by NPY was significantly higher in endothelium-intact rings compared to endotheliumdenuded rings obtained after 30 and 60 min of carotid occlusion. Conclusion. The present investigation provides results of increased vasocontractile effect of NOR and decreased enhancing effect of NPY on NOR vasoconstriction in the rabbit facial artery after carotid occlusion that is related to altered endothelium function., Uvod/Cilj. Pokazano je da se okluzivne promene aterosklerotične prirode mogu opaziti na orofacijalnim granama spoljne karotidne arterije. Ishemija izazvana aterosklerozom, dovodi do poremećaja stvaranja i oslobađanja faktora poreklom iz endotela. Cilj ovog istraživanja bio je da se ispita uticaj okluzije karotidne arterije (10, 30 i 60 min) na vazokontraktilne efekte norepinefrina (NOR) i neuropeptida Y (NPY) na izolovanoj žlezdanoj grani facijalne arterije kunića, glavne dovodne arterije za submandibularnu žlezdu. Metode. U kupatilu za izolovane krvne sudove ispitivane su izometrijske promene tonusa arterijskih preparata, pre i posle okluzije karotidne arterije. Rezultati. Koncentracijski-zavisan vazokontraktilni efekat NOR bio je značajno veći posle 30 i 60 min karotidne okluzije, ali samo na preparatima sa očuvanim endotelom. Primenjen u rastućim koncentracijama, NPY nije imao efekta na tonus izolovane žlezdane grane facijalne aterije kunića, ali je povećao vazokontraktilni efekat NOR na svim ispitivanim preparatima. Povećanje vazokontraktilnog efekta NOR u prisustvu NPY bilo je značajno manje posle 30 i 60 min okluzije. Takođe, efekat NPY posle karotidne okluzije bio je značajno veći na preparatima sa očuvanim endotelom u odnosu na preparate sa uklonjenim endotelom. Zaključak. Ovo istraživanje pokazalo je povećanje vazokontraktilnog efekta NOR i smanjenje potencirajućeg efekta NPY na vazokonstrikciju izazvanu NOR na facijalnoj arteriji kunića posle okluzije karotidne arterije koje su povezane sa promenjenom funkcijom endotela.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Effect of neuropeptide Y on norepinephrine-induced constriction in the rabbit facial artery after carotid artery occlusion, Efekat neuropeptida Y na konstrikciju facijalne arterije kunića izazvane norepinefrinom posle okluzije karotidne arterije",
volume = "71",
number = "6",
pages = "571-575",
doi = "10.2298/VSP1406571R"
}

Roganović, J., Petrović, N.,& Đukić, L.. (2014). Effect of neuropeptide Y on norepinephrine-induced constriction in the rabbit facial artery after carotid artery occlusion. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 71(6), 571-575.
https://doi.org/10.2298/VSP1406571R

Roganović J, Petrović N, Đukić L. Effect of neuropeptide Y on norepinephrine-induced constriction in the rabbit facial artery after carotid artery occlusion. in Vojnosanitetski pregled. 2014;71(6):571-575.
doi:10.2298/VSP1406571R .

Roganović, Jelena, Petrović, Nina, Đukić, Ljiljana, "Effect of neuropeptide Y on norepinephrine-induced constriction in the rabbit facial artery after carotid artery occlusion" in Vojnosanitetski pregled, 71, no. 6 (2014):571-575,
https://doi.org/10.2298/VSP1406571R . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Mandusić, Vesna
AU  - Stanojević, Boban
AU  - Lukić, Silvana
AU  - Todorović, Lidija
AU  - Roganović, Jelena
AU  - Dimitrijević, Bogomir
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1859
AB  - MicroRNA-21 (miR-21) overexpression is characteristic for various types of tumors, but it is still unknown whether its expression levels differ between invasive and non-invasive breast carcinomas. The main goal of the study was to determine the difference in miR-21 expression among normal tissue, non-invasive, invasive with non-invasive component, and pure invasive breast cancer samples, to explain its potential role and significance in breast cancer invasiveness. The second goal was to propose miR-21 as molecular marker of breast cancer invasiveness and potential target for future anti-miR therapies for the prevention of invasion and metastasis. In order to reveal the role of miR-21 in breast cancer invasiveness, we measured miR-21 expression levels in 44 breast cancer and four normal samples by stem-loop real-time RT-PCR using TaqMan technology. Relative expression levels of miR-21 were significantly higher in invasive than in other groups (P = 0.002) and significantly higher in invasive compared with invasive with non-invasive component group in histological (P = 0.043) and nuclear grade 2 (P = 0.036), estrogen-receptor-positive (ER+) (P = 0.006), progesterone-receptor-positive (PR+) (P = 0.008), ER+ PR+ (P = 0.007), and proliferation index (Ki-67)  lt = 20 % (P = 0.036) tumors. Our findings suggest that miR-21 could be independent molecular marker of breast cancer invasiveness and potential target for future anti-miR therapies for the prevention of invasion and metastasis.
PB  - Humana Press Inc, Totowa
T2  - Medical Oncology
T1  - The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion
VL  - 31
IS  - 3
DO  - 10.1007/s12032-014-0867-x
ER  - 

@article{
author = "Petrović, Nina and Mandusić, Vesna and Stanojević, Boban and Lukić, Silvana and Todorović, Lidija and Roganović, Jelena and Dimitrijević, Bogomir",
year = "2014",
abstract = "MicroRNA-21 (miR-21) overexpression is characteristic for various types of tumors, but it is still unknown whether its expression levels differ between invasive and non-invasive breast carcinomas. The main goal of the study was to determine the difference in miR-21 expression among normal tissue, non-invasive, invasive with non-invasive component, and pure invasive breast cancer samples, to explain its potential role and significance in breast cancer invasiveness. The second goal was to propose miR-21 as molecular marker of breast cancer invasiveness and potential target for future anti-miR therapies for the prevention of invasion and metastasis. In order to reveal the role of miR-21 in breast cancer invasiveness, we measured miR-21 expression levels in 44 breast cancer and four normal samples by stem-loop real-time RT-PCR using TaqMan technology. Relative expression levels of miR-21 were significantly higher in invasive than in other groups (P = 0.002) and significantly higher in invasive compared with invasive with non-invasive component group in histological (P = 0.043) and nuclear grade 2 (P = 0.036), estrogen-receptor-positive (ER+) (P = 0.006), progesterone-receptor-positive (PR+) (P = 0.008), ER+ PR+ (P = 0.007), and proliferation index (Ki-67)  lt = 20 % (P = 0.036) tumors. Our findings suggest that miR-21 could be independent molecular marker of breast cancer invasiveness and potential target for future anti-miR therapies for the prevention of invasion and metastasis.",
publisher = "Humana Press Inc, Totowa",
journal = "Medical Oncology",
title = "The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion",
volume = "31",
number = "3",
doi = "10.1007/s12032-014-0867-x"
}

Petrović, N., Mandusić, V., Stanojević, B., Lukić, S., Todorović, L., Roganović, J.,& Dimitrijević, B.. (2014). The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion. in Medical Oncology
Humana Press Inc, Totowa., 31(3).
https://doi.org/10.1007/s12032-014-0867-x

Petrović N, Mandusić V, Stanojević B, Lukić S, Todorović L, Roganović J, Dimitrijević B. The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion. in Medical Oncology. 2014;31(3).
doi:10.1007/s12032-014-0867-x .

Petrović, Nina, Mandusić, Vesna, Stanojević, Boban, Lukić, Silvana, Todorović, Lidija, Roganović, Jelena, Dimitrijević, Bogomir, "The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion" in Medical Oncology, 31, no. 3 (2014),
https://doi.org/10.1007/s12032-014-0867-x . .

TY  - JOUR
AU  - Ilić, Jugoslav
AU  - Radović, Katarina
AU  - Roganović, Jelena
AU  - Brković, Božidar
AU  - Stojić, Dragica
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1735
AB  - Introduction: Vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP 2) are growth factors (GFs) identified within the dentine-pulp complex and involved into the cellular events connected to the pulp-healing response. It is well established that the expression of these GFs is increased in different tissues in diabetes mellitus. Because there are no data concerning the levels of VEGF and BMP 2 in human dental pulp, the aim of present study was to quantify VEGF and BMP 2 levels in intact dental pulp and dental pulp that underwent reactive dentinogenesis in healthy and diabetic human subjects. Methods: The study was conducted on 28 healthy and 28 subjects with controlled diabetes type II who underwent pulp extirpation as a part of prosthetic rehabilitation. Pulp were collected from intact teeth and teeth treated by indirect pulp capping. The levels of VEGF and BMP 2 were determined in the pulp tissue lysates with enzyme-linked immunosorbent assay. Results: The levels of VEGF and BMP 2 were significantly higher in intact teeth pulp of diabetic than in healthy subjects. The concentrations of these GFs were significantly lowered in teeth with indirect pulp capping both in healthy and diabetic persons. Furthermore, VEGF and BMP 2 levels were in strong positive correlation. Conclusions: Similar changes in the levels of VEGF and BMP 2 in intact and treated teeth of healthy and diabetic patients could be suggestive of associated roles of these GFs in responses of healthy and diabetic dental pulp. (J Endod 2012;38:764-768)
PB  - Elsevier Science Inc, New York
T2  - Journal of Endodontics
T1  - The Levels of Vascular Endothelial Growth Factor and Bone Morphogenetic Protein 2 in Dental Pulp Tissue of Healthy and Diabetic Patients
VL  - 38
IS  - 6
SP  - 764
EP  - 768
DO  - 10.1016/j.joen.2012.03.016
ER  - 

@article{
author = "Ilić, Jugoslav and Radović, Katarina and Roganović, Jelena and Brković, Božidar and Stojić, Dragica",
year = "2012",
abstract = "Introduction: Vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP 2) are growth factors (GFs) identified within the dentine-pulp complex and involved into the cellular events connected to the pulp-healing response. It is well established that the expression of these GFs is increased in different tissues in diabetes mellitus. Because there are no data concerning the levels of VEGF and BMP 2 in human dental pulp, the aim of present study was to quantify VEGF and BMP 2 levels in intact dental pulp and dental pulp that underwent reactive dentinogenesis in healthy and diabetic human subjects. Methods: The study was conducted on 28 healthy and 28 subjects with controlled diabetes type II who underwent pulp extirpation as a part of prosthetic rehabilitation. Pulp were collected from intact teeth and teeth treated by indirect pulp capping. The levels of VEGF and BMP 2 were determined in the pulp tissue lysates with enzyme-linked immunosorbent assay. Results: The levels of VEGF and BMP 2 were significantly higher in intact teeth pulp of diabetic than in healthy subjects. The concentrations of these GFs were significantly lowered in teeth with indirect pulp capping both in healthy and diabetic persons. Furthermore, VEGF and BMP 2 levels were in strong positive correlation. Conclusions: Similar changes in the levels of VEGF and BMP 2 in intact and treated teeth of healthy and diabetic patients could be suggestive of associated roles of these GFs in responses of healthy and diabetic dental pulp. (J Endod 2012;38:764-768)",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Endodontics",
title = "The Levels of Vascular Endothelial Growth Factor and Bone Morphogenetic Protein 2 in Dental Pulp Tissue of Healthy and Diabetic Patients",
volume = "38",
number = "6",
pages = "764-768",
doi = "10.1016/j.joen.2012.03.016"
}

Ilić, J., Radović, K., Roganović, J., Brković, B.,& Stojić, D.. (2012). The Levels of Vascular Endothelial Growth Factor and Bone Morphogenetic Protein 2 in Dental Pulp Tissue of Healthy and Diabetic Patients. in Journal of Endodontics
Elsevier Science Inc, New York., 38(6), 764-768.
https://doi.org/10.1016/j.joen.2012.03.016

Ilić J, Radović K, Roganović J, Brković B, Stojić D. The Levels of Vascular Endothelial Growth Factor and Bone Morphogenetic Protein 2 in Dental Pulp Tissue of Healthy and Diabetic Patients. in Journal of Endodontics. 2012;38(6):764-768.
doi:10.1016/j.joen.2012.03.016 .

Ilić, Jugoslav, Radović, Katarina, Roganović, Jelena, Brković, Božidar, Stojić, Dragica, "The Levels of Vascular Endothelial Growth Factor and Bone Morphogenetic Protein 2 in Dental Pulp Tissue of Healthy and Diabetic Patients" in Journal of Endodontics, 38, no. 6 (2012):764-768,
https://doi.org/10.1016/j.joen.2012.03.016 . .

TY  - CHAP
AU  - Stojić, Dragica
AU  - Roganović, Jelena
AU  - Brković, Božidar
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2593
AB  - Branches of the external carotid artery support the integrity and functionality of oral tissue beds with critical impact in both health and disease. Stenosis or occlusion of the common carotid artery or of the tributaries – facial, maxillary, lingual arteries and dental pulp arterioles – contribute to the etiology of disease, in particular in the setting of ischemic and metabolic syndrome disorders of orofacial tissues.
Blood flow through salivary glands is largely controlled via autonomic innervation, and endothelium-derived vasodilating and vasoconstricting substances. Endothelial cells actively regulate basal vascular tone and vascular reactivity in physiological and pathological conditions. In the resting state, blood flow in rat submandibular gland is largely controlled by sympathetic nerves, but vascular resistance is rapidly lowered by parasympathetic activity. Increase in blood flow during parasympathetic stimulation, through co-release of the neurotransmitters Ach and VIP, has been attributed to the release of endothelium-derived NO, prostacyclin and EDHF. In the glandular branch of rabbit facial artery, Ach provokes endothelium-dependent vasorelaxation, mediated by NO and prostacyclin, while VIP induces an endothelium-independent vasorelaxant effect, mediated by cAMP from vascular smooth muscle and neuronal NO. In the human submandibular artery, both transmitters produce endothelium-dependent vasodilation with different mechanisms, release of NO and prostacyclin for Ach and release of NO and EDHF for VIP.
Endothelial dysfunction is commonly attributed to impaired endothelium-dependent vasorelaxation and increased vascular tone. In diabetic rat salivary glands, parasympathetic stimulation causes increased blood flow with delayed initial response. Carotid artery occlusion decreases the responsiveness of the glandular branch of the rabbit facial artery to Ach and VIP, with altered endothelial, smooth muscle and neuronal signaling.
The dental pulp is a highly vascularized tissue, richly supplied with vasomotor nerves of sensory and sympathetic origin. A major difference between the vascularization of the pulp versus other tissues is the low-compliance since in the pulp vasodilation might lead to necrosis due to secondary vasoconstriction, whereas vasodilation typically in other tissues promotes survival during inflammation. Catecholamines are an important element in neuronal and humoral regulation of pulpal blood flow. Administration of catecholamines with local anesthetics causes sufficient vasoconstriction to disturb the pulpal microcirculation in dental patients. Endothelial NOS was detected in endothelium of the rat pulp microvasculature and it is likely that NO regulates pulpal vasodilation through a cGMP-dependent mechanism. NO synthesized from nerve fibers and odontoblasts could also reach endothelial and vascular smooth muscle cells of the pulp to regulate blood vessel function.
In contrast to vasoconstriction of pulpal blood vessels, vasoconstriction of maxillary artery branches is useful for effective and long intraoral anesthesia. Local anesthetics are vasodilators, leading to decreased anesthetic effect, except ropivacaine which exert vasoconstriction on isolated infraorbital branches of human maxillary artery. To oppose the vasodilatory action of local anesthetics epinephrine is added to local anesthetic solutions. However, because of its absorption from injection sites, there are some limitations in the cardiovascularly compromised patients. It has been shown that clonidine, an alpha 2 adrenergic agonist, is a safer vasoconstrictor than epinephrine, providing hemodynamic stability due to its central hypotensive action.
PB  - Nova Science Publishers, Inc.
T2  - Advances in Medicine and Biology. Volume 29
T1  - Functionality of Orofacial Branches Feeding: Salivary Glands, Dental Pulp and Intraoral Anesthetic Field
SP  - 59
EP  - 95
UR  - https://hdl.handle.net/21.15107/rcub_smile_2593
ER  - 

@inbook{
author = "Stojić, Dragica and Roganović, Jelena and Brković, Božidar",
year = "2012",
abstract = "Branches of the external carotid artery support the integrity and functionality of oral tissue beds with critical impact in both health and disease. Stenosis or occlusion of the common carotid artery or of the tributaries – facial, maxillary, lingual arteries and dental pulp arterioles – contribute to the etiology of disease, in particular in the setting of ischemic and metabolic syndrome disorders of orofacial tissues.
Blood flow through salivary glands is largely controlled via autonomic innervation, and endothelium-derived vasodilating and vasoconstricting substances. Endothelial cells actively regulate basal vascular tone and vascular reactivity in physiological and pathological conditions. In the resting state, blood flow in rat submandibular gland is largely controlled by sympathetic nerves, but vascular resistance is rapidly lowered by parasympathetic activity. Increase in blood flow during parasympathetic stimulation, through co-release of the neurotransmitters Ach and VIP, has been attributed to the release of endothelium-derived NO, prostacyclin and EDHF. In the glandular branch of rabbit facial artery, Ach provokes endothelium-dependent vasorelaxation, mediated by NO and prostacyclin, while VIP induces an endothelium-independent vasorelaxant effect, mediated by cAMP from vascular smooth muscle and neuronal NO. In the human submandibular artery, both transmitters produce endothelium-dependent vasodilation with different mechanisms, release of NO and prostacyclin for Ach and release of NO and EDHF for VIP.
Endothelial dysfunction is commonly attributed to impaired endothelium-dependent vasorelaxation and increased vascular tone. In diabetic rat salivary glands, parasympathetic stimulation causes increased blood flow with delayed initial response. Carotid artery occlusion decreases the responsiveness of the glandular branch of the rabbit facial artery to Ach and VIP, with altered endothelial, smooth muscle and neuronal signaling.
The dental pulp is a highly vascularized tissue, richly supplied with vasomotor nerves of sensory and sympathetic origin. A major difference between the vascularization of the pulp versus other tissues is the low-compliance since in the pulp vasodilation might lead to necrosis due to secondary vasoconstriction, whereas vasodilation typically in other tissues promotes survival during inflammation. Catecholamines are an important element in neuronal and humoral regulation of pulpal blood flow. Administration of catecholamines with local anesthetics causes sufficient vasoconstriction to disturb the pulpal microcirculation in dental patients. Endothelial NOS was detected in endothelium of the rat pulp microvasculature and it is likely that NO regulates pulpal vasodilation through a cGMP-dependent mechanism. NO synthesized from nerve fibers and odontoblasts could also reach endothelial and vascular smooth muscle cells of the pulp to regulate blood vessel function.
In contrast to vasoconstriction of pulpal blood vessels, vasoconstriction of maxillary artery branches is useful for effective and long intraoral anesthesia. Local anesthetics are vasodilators, leading to decreased anesthetic effect, except ropivacaine which exert vasoconstriction on isolated infraorbital branches of human maxillary artery. To oppose the vasodilatory action of local anesthetics epinephrine is added to local anesthetic solutions. However, because of its absorption from injection sites, there are some limitations in the cardiovascularly compromised patients. It has been shown that clonidine, an alpha 2 adrenergic agonist, is a safer vasoconstrictor than epinephrine, providing hemodynamic stability due to its central hypotensive action.",
publisher = "Nova Science Publishers, Inc.",
journal = "Advances in Medicine and Biology. Volume 29",
booktitle = "Functionality of Orofacial Branches Feeding: Salivary Glands, Dental Pulp and Intraoral Anesthetic Field",
pages = "59-95",
url = "https://hdl.handle.net/21.15107/rcub_smile_2593"
}

Stojić, D., Roganović, J.,& Brković, B.. (2012). Functionality of Orofacial Branches Feeding: Salivary Glands, Dental Pulp and Intraoral Anesthetic Field. in Advances in Medicine and Biology. Volume 29
Nova Science Publishers, Inc.., 59-95.
https://hdl.handle.net/21.15107/rcub_smile_2593

Stojić D, Roganović J, Brković B. Functionality of Orofacial Branches Feeding: Salivary Glands, Dental Pulp and Intraoral Anesthetic Field. in Advances in Medicine and Biology. Volume 29. 2012;:59-95.
https://hdl.handle.net/21.15107/rcub_smile_2593 .

Stojić, Dragica, Roganović, Jelena, Brković, Božidar, "Functionality of Orofacial Branches Feeding: Salivary Glands, Dental Pulp and Intraoral Anesthetic Field" in Advances in Medicine and Biology. Volume 29 (2012):59-95,
https://hdl.handle.net/21.15107/rcub_smile_2593 .

TY  - THES
AU  - Roganović, Jelena
PY  - 2011
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2592
AB  - The aim of this study was to assess, on the experimental model of rabbit, the effect of type 1 diabetes mellitus (induced by a single intravenous injection of 100 mg kg−1 of alloxan) on vascular tonus of parotid artery, feeding artery for parotid gland, and secretory activity of this gland, induced by stimulation of cholinergic muscarinic receptors (by acetylcholine -ACh and carbachol, respectively). Vasorelaxant effect was investigated by isometric force measurements in parotid artery rings while salivary flow was registered collecting the saliva after iv application of carbachol. Quantification of inducible nitric oxide synthase (iNOS) mRNA expression was made in rabbit parotid artery and gland by Real-time RT-PCR. Also, basal tissue concentrations of cGMP in parotid artery and gland were determined by ELISA. 
Acetylcholine induced concentration- and endothelium-dependent vasorelaxation that was significantly decreased in parotid artery rings from diabetic rabbit compared to controls. Schild analysis of the ACh vasorelaxant effect, in the presence of selective muscarinic receptor antagonists, revealed involvement of the M3 receptor subtype in parotid artery rings from both control and diabetic rabbits, with no change in antagonist affinity constants. The inhibitory effects of indomethacin, a non-selective inhibitor of cyclooxygenase, and of high potassium, an inhibitor of hyperpolarization, on ACh vasorelaxation were increased. The effect of L-NOARG, a non-selective inhibitor of NOS, was decreased in diabetic parotid artery while  SMT, a selective inhibitor of iNOS, significantly reduced ACh vasorelaxation only in parotid artery rings from diabetic rabbits. Carbachol induced dose-dependent increase in parotid gland secretion, being significantly reduced in the diabetic rabbits. Functional studies with the selective muscarinic receptor antagonists revealed involvement of the solely M3 receptor subtype in carbachol-induced parotid gland secretion from both control and diabetic rabbits. Inhibitory effects of   Nω, a selective inhibitor of nNOS, as well as of L-NOARG and SMT on carbachol-induced parotid secretion were increased in diabetes. Also, up-regulation of iNOS mRNA expression and basal cGMP concentration were detected in parotid artery rings and glands from diabetic rabbits. These results suggest that decreased  vasorelaxant effect of ACh in parotid artery and sialogouge effect of carbachol in parotid gland of diabetic rabbits are the consequence of impaired signal transduction mechanisms related to activation of cholinergic M3  receptors: disturbed balance between different NOS isoforms, but not a consequence of altered affinity and number of  M3 receptors.
AB  - Cilj ovog rada je bio da se na eksperimentalnom modelu kunića ispita uticaj dijabetes melitusa tipa 1, izazvanog  jednom intravenskom injekcijom aloksana (100mg/kg), na vaskularni tonus parotidne arterije, koja ishranjuje parotidnu žlezdu i sekretornu aktivnost ove žlezde, izazvane stimulacijom holinergičkih muskarinskih receptora (acetilholinom –ACh, odnosno karbaholom).
 Vazorelaksantni efekt je registrovan izometrijskim promenama u tonusu arterijskih prstenova, a protok pljuvačke je registrovan sakupljanjem pljuvačke posle intravenske primene karbahola. Takođe, u tkivu parotidne arterije i žlezde kvantifikovana je: ekspresija mRNK iNOS, metodom Real-time RT-PCR, i bazalna koncentracija cGMP, imunoesejskom metodom (ELISA).
Dobijeni rezultati pokazuju da ACh izaziva koncentracijski i endotelno-zavisnu relaksaciju parotidne arterije koja je značajno manja na arterijama kunića sa dijabetesom u odnosu na kontrolnu grupu životinja. Šildova analiza efekta ACh, u prisustvu selektivnih muskarinskih antagonista, pokazuje da su samo M3 receptori uključeni u ovaj efekt ACh, i to kako kod kontrolnih tako i kod kunića sa dijabetesom. Inhibitorni efekti indometacina, neselektivnog inhibitora COX, i visokih koncentracija kalijuma na vazorelaksantnom dejstvu ACh rastu, a efekt L-NOARG, neselektivnog inhibitora NOS, se smanjuje na parotidnim arterijama kunića sa dijabetesom. Takođe, primena SMT, selektivnog inhibitora iNOS, značajno smanjuje vazorelaksantni efekt ACh samo na parotidnim arterijama kunića sa dijabetesom. Karbahol prouzrokuje dozno-zavisno povećanje sekrecije parotidne pljuvačke, koja je značajno smanjena u dijabetesu. Funkcionalna ispitivanja primenom selektivnih muskarinskih antagonista pokazuje učešće samo M3 receptora u parotidnoj sekreciji, izazvanoj karbaholom, i to kako kod kontrolnih tako i kod kunića sa dijabetesom. Takođe, u tkivu parotidne arterije i žlezde kunića sa dijabetesom značajno se  povećava ekspresija mRNK iNOS kao i bazalne koncentracije cGMP. Na osnovu ovih rezultata može se zaključiti da  smanjenje vazorelaksantnog efekta ACh na parotidnoj arteriji, i sekretornog efekta karbahola na parotidnoj žlezdi kunića sa dijabetesom su posledica poremećaja u signalnim transdukcionim mehanizmima vezanim za aktivaciju holinergičkih M3 receptora: poremećene ravnoteže između aktivnosti pojedinih NOS izoformi,  a nisu posledica promena na nivou afiniteta i broja M3 muskarinskih receptora.
PB  - Univerzitet u Beogradu, Medicinski fakultet
T1  - Modulatory role of nitric oxide in the muscarinic receptor-induced vasodilation and secretion in the parotid gland of diabetic rabbit
T1  - Modulatorna uloga azot monoksida u muskarinskoj vazodilataciji i sekreciji  parotidne žlezde kunića sa dijabetesom
UR  - https://hdl.handle.net/21.15107/rcub_smile_2592
ER  - 

@phdthesis{
author = "Roganović, Jelena",
year = "2011",
abstract = "The aim of this study was to assess, on the experimental model of rabbit, the effect of type 1 diabetes mellitus (induced by a single intravenous injection of 100 mg kg−1 of alloxan) on vascular tonus of parotid artery, feeding artery for parotid gland, and secretory activity of this gland, induced by stimulation of cholinergic muscarinic receptors (by acetylcholine -ACh and carbachol, respectively). Vasorelaxant effect was investigated by isometric force measurements in parotid artery rings while salivary flow was registered collecting the saliva after iv application of carbachol. Quantification of inducible nitric oxide synthase (iNOS) mRNA expression was made in rabbit parotid artery and gland by Real-time RT-PCR. Also, basal tissue concentrations of cGMP in parotid artery and gland were determined by ELISA. 
Acetylcholine induced concentration- and endothelium-dependent vasorelaxation that was significantly decreased in parotid artery rings from diabetic rabbit compared to controls. Schild analysis of the ACh vasorelaxant effect, in the presence of selective muscarinic receptor antagonists, revealed involvement of the M3 receptor subtype in parotid artery rings from both control and diabetic rabbits, with no change in antagonist affinity constants. The inhibitory effects of indomethacin, a non-selective inhibitor of cyclooxygenase, and of high potassium, an inhibitor of hyperpolarization, on ACh vasorelaxation were increased. The effect of L-NOARG, a non-selective inhibitor of NOS, was decreased in diabetic parotid artery while  SMT, a selective inhibitor of iNOS, significantly reduced ACh vasorelaxation only in parotid artery rings from diabetic rabbits. Carbachol induced dose-dependent increase in parotid gland secretion, being significantly reduced in the diabetic rabbits. Functional studies with the selective muscarinic receptor antagonists revealed involvement of the solely M3 receptor subtype in carbachol-induced parotid gland secretion from both control and diabetic rabbits. Inhibitory effects of   Nω, a selective inhibitor of nNOS, as well as of L-NOARG and SMT on carbachol-induced parotid secretion were increased in diabetes. Also, up-regulation of iNOS mRNA expression and basal cGMP concentration were detected in parotid artery rings and glands from diabetic rabbits. These results suggest that decreased  vasorelaxant effect of ACh in parotid artery and sialogouge effect of carbachol in parotid gland of diabetic rabbits are the consequence of impaired signal transduction mechanisms related to activation of cholinergic M3  receptors: disturbed balance between different NOS isoforms, but not a consequence of altered affinity and number of  M3 receptors., Cilj ovog rada je bio da se na eksperimentalnom modelu kunića ispita uticaj dijabetes melitusa tipa 1, izazvanog  jednom intravenskom injekcijom aloksana (100mg/kg), na vaskularni tonus parotidne arterije, koja ishranjuje parotidnu žlezdu i sekretornu aktivnost ove žlezde, izazvane stimulacijom holinergičkih muskarinskih receptora (acetilholinom –ACh, odnosno karbaholom).
 Vazorelaksantni efekt je registrovan izometrijskim promenama u tonusu arterijskih prstenova, a protok pljuvačke je registrovan sakupljanjem pljuvačke posle intravenske primene karbahola. Takođe, u tkivu parotidne arterije i žlezde kvantifikovana je: ekspresija mRNK iNOS, metodom Real-time RT-PCR, i bazalna koncentracija cGMP, imunoesejskom metodom (ELISA).
Dobijeni rezultati pokazuju da ACh izaziva koncentracijski i endotelno-zavisnu relaksaciju parotidne arterije koja je značajno manja na arterijama kunića sa dijabetesom u odnosu na kontrolnu grupu životinja. Šildova analiza efekta ACh, u prisustvu selektivnih muskarinskih antagonista, pokazuje da su samo M3 receptori uključeni u ovaj efekt ACh, i to kako kod kontrolnih tako i kod kunića sa dijabetesom. Inhibitorni efekti indometacina, neselektivnog inhibitora COX, i visokih koncentracija kalijuma na vazorelaksantnom dejstvu ACh rastu, a efekt L-NOARG, neselektivnog inhibitora NOS, se smanjuje na parotidnim arterijama kunića sa dijabetesom. Takođe, primena SMT, selektivnog inhibitora iNOS, značajno smanjuje vazorelaksantni efekt ACh samo na parotidnim arterijama kunića sa dijabetesom. Karbahol prouzrokuje dozno-zavisno povećanje sekrecije parotidne pljuvačke, koja je značajno smanjena u dijabetesu. Funkcionalna ispitivanja primenom selektivnih muskarinskih antagonista pokazuje učešće samo M3 receptora u parotidnoj sekreciji, izazvanoj karbaholom, i to kako kod kontrolnih tako i kod kunića sa dijabetesom. Takođe, u tkivu parotidne arterije i žlezde kunića sa dijabetesom značajno se  povećava ekspresija mRNK iNOS kao i bazalne koncentracije cGMP. Na osnovu ovih rezultata može se zaključiti da  smanjenje vazorelaksantnog efekta ACh na parotidnoj arteriji, i sekretornog efekta karbahola na parotidnoj žlezdi kunića sa dijabetesom su posledica poremećaja u signalnim transdukcionim mehanizmima vezanim za aktivaciju holinergičkih M3 receptora: poremećene ravnoteže između aktivnosti pojedinih NOS izoformi,  a nisu posledica promena na nivou afiniteta i broja M3 muskarinskih receptora.",
publisher = "Univerzitet u Beogradu, Medicinski fakultet",
title = "Modulatory role of nitric oxide in the muscarinic receptor-induced vasodilation and secretion in the parotid gland of diabetic rabbit, Modulatorna uloga azot monoksida u muskarinskoj vazodilataciji i sekreciji  parotidne žlezde kunića sa dijabetesom",
url = "https://hdl.handle.net/21.15107/rcub_smile_2592"
}

Roganović, J.. (2011). Modulatory role of nitric oxide in the muscarinic receptor-induced vasodilation and secretion in the parotid gland of diabetic rabbit. 
Univerzitet u Beogradu, Medicinski fakultet..
https://hdl.handle.net/21.15107/rcub_smile_2592

Roganović J. Modulatory role of nitric oxide in the muscarinic receptor-induced vasodilation and secretion in the parotid gland of diabetic rabbit. 2011;.
https://hdl.handle.net/21.15107/rcub_smile_2592 .

Roganović, Jelena, "Modulatory role of nitric oxide in the muscarinic receptor-induced vasodilation and secretion in the parotid gland of diabetic rabbit" (2011),
https://hdl.handle.net/21.15107/rcub_smile_2592 .

TY  - JOUR
AU  - Roganović, Jelena
AU  - Radenković, Miroslav
AU  - Tanić, Nikola
AU  - Tanić, Nasta
AU  - Petrović, Nina
AU  - Stojić, Dragica
PY  - 2011
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1625
AB  - The aim of this study was to assess the effect of type 1 diabetes mellitus (induced by a single intravenous injection of 100 mg kg(-1) of alloxan) on acetylcholine (ACh)-induced relaxation in isolated rabbit parotid gland feeding artery. Isometric force measurements and quantification of inducible nitric oxide synthase (iNOS) mRNA by real-time RT-PCR were made in parotid artery rings from diabetic and control rabbits. Acetylcholine induced concentration- and endothelium-dependent vasorelaxation that was significantly decreased in parotid artery rings from diabetic rabbits. Schild analysis of the ACh vasorelaxant effect, in the presence of selective muscarinic receptor antagonists, revealed involvement of the M-3 receptor subtype in parotid artery rings from both control and diabetic rabbits, with no change in antagonist affinity constants. The inhibitory effects of indomethacin, a non-selective inhibitor of cyclooxygenase, and of high potassium, an inhibitor of hyperpolarization, on ACh vasorelaxation were increased. The effect of N-G-nitro-L-arginine, a non-selective inhibitor of NOS, was decreased in diabetes. S-methylisothiourea, a selective inhibitor of iNOS, significantly reduced ACh vasorelaxation only in parotid artery rings from diabetic rabbits. Also, up-regulation of iNOS mRNA expression was detected in parotid artery rings from diabetic rabbits. These results suggest that in parotid artery rings from diabetic rabbits, impaired endothelium-dependent vasorelaxation to ACh appears to be caused by the loss of a nitric oxide-mediated component and increased iNOS expression, and is unlikely to be caused by a change at the M-3 receptor level.
PB  - Wiley, Hoboken
T2  - European Journal of Oral Sciences
T1  - Impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbit
VL  - 119
IS  - 5
SP  - 352
EP  - 360
DO  - 10.1111/j.1600-0722.2011.00851.x
ER  - 

@article{
author = "Roganović, Jelena and Radenković, Miroslav and Tanić, Nikola and Tanić, Nasta and Petrović, Nina and Stojić, Dragica",
year = "2011",
abstract = "The aim of this study was to assess the effect of type 1 diabetes mellitus (induced by a single intravenous injection of 100 mg kg(-1) of alloxan) on acetylcholine (ACh)-induced relaxation in isolated rabbit parotid gland feeding artery. Isometric force measurements and quantification of inducible nitric oxide synthase (iNOS) mRNA by real-time RT-PCR were made in parotid artery rings from diabetic and control rabbits. Acetylcholine induced concentration- and endothelium-dependent vasorelaxation that was significantly decreased in parotid artery rings from diabetic rabbits. Schild analysis of the ACh vasorelaxant effect, in the presence of selective muscarinic receptor antagonists, revealed involvement of the M-3 receptor subtype in parotid artery rings from both control and diabetic rabbits, with no change in antagonist affinity constants. The inhibitory effects of indomethacin, a non-selective inhibitor of cyclooxygenase, and of high potassium, an inhibitor of hyperpolarization, on ACh vasorelaxation were increased. The effect of N-G-nitro-L-arginine, a non-selective inhibitor of NOS, was decreased in diabetes. S-methylisothiourea, a selective inhibitor of iNOS, significantly reduced ACh vasorelaxation only in parotid artery rings from diabetic rabbits. Also, up-regulation of iNOS mRNA expression was detected in parotid artery rings from diabetic rabbits. These results suggest that in parotid artery rings from diabetic rabbits, impaired endothelium-dependent vasorelaxation to ACh appears to be caused by the loss of a nitric oxide-mediated component and increased iNOS expression, and is unlikely to be caused by a change at the M-3 receptor level.",
publisher = "Wiley, Hoboken",
journal = "European Journal of Oral Sciences",
title = "Impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbit",
volume = "119",
number = "5",
pages = "352-360",
doi = "10.1111/j.1600-0722.2011.00851.x"
}

Roganović, J., Radenković, M., Tanić, N., Tanić, N., Petrović, N.,& Stojić, D.. (2011). Impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbit. in European Journal of Oral Sciences
Wiley, Hoboken., 119(5), 352-360.
https://doi.org/10.1111/j.1600-0722.2011.00851.x

Roganović J, Radenković M, Tanić N, Tanić N, Petrović N, Stojić D. Impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbit. in European Journal of Oral Sciences. 2011;119(5):352-360.
doi:10.1111/j.1600-0722.2011.00851.x .

Roganović, Jelena, Radenković, Miroslav, Tanić, Nikola, Tanić, Nasta, Petrović, Nina, Stojić, Dragica, "Impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbit" in European Journal of Oral Sciences, 119, no. 5 (2011):352-360,
https://doi.org/10.1111/j.1600-0722.2011.00851.x . .