TY  - JOUR
AU  - Velicković, Milena
AU  - Pejnović, Nada
AU  - Petrović, Renata
AU  - Mitrović, Slobodanka
AU  - Jeftić, Ilija
AU  - Kanjevac, Tatjana
AU  - Lukić, Aleksandra
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2135
AB  - BACKGROUND: Interleukin-33 (IL-33) is a recently identified cytokine belonging to the IL-1 family and ligand for the IL-1 receptor-related protein ST2. IL-33/ST2 signaling plays a critical role in allergy, autoimmunity, and chronic inflammatory disorders, but its role in the pathogenesis of periapical lesions is unknown. We aimed to investigate the expression patterns of IL-33 and ST2 in human periapical lesions. METHODS: Periapical lesions (n = 36) and healthy periapical tissues (n = 10) were evaluated by immunohistochemistry using antibodies specific for human IL-33 and ST2. Lesion samples were further analyzed by double immunofluorescence to assess IL-33/ST2 co-expression. RESULTS: The numbers of IL-33- and ST2-positive fibroblasts were significantly higher in periapical lesions compared to healthy periapical tissues (both P  lt  0.05), while the numbers of IL-33-and ST2-positive endothelial cells were similar (both P > 0.05). There were no significant differences in the numbers of IL-33-and ST2-positive fibroblasts and endothelial cells between periapical granulomas and radicular cysts (all P > 0.05). Similarly, numbers of ST2-positive mononuclear cells did not differ between periapical granulomas and radicular cysts (P > 0.05). The majority of epithelial cells in radicular cysts were IL-33 positive, while the small proportion of epithelial cells was ST2 positive. Double immunofluorescence analysis revealed IL-33/ST2 co-expression in fibroblasts and endothelial cells. CONCLUSIONS: IL-33 and ST2 are expressed in periapical granulomas and radicular cysts. Increased numbers of IL-33-and ST2-positive fibroblasts in periapical lesions when compared to healthy periapical tissues suggest that IL-33/ST2 signaling may be involved in periapical inflammation and tissue fibrosis.
PB  - Wiley, Hoboken
T2  - Journal of Oral Pathology & Medicine
T1  - Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts
VL  - 45
IS  - 1
SP  - 70
EP  - 76
DO  - 10.1111/jop.12312
ER  - 

@article{
author = "Velicković, Milena and Pejnović, Nada and Petrović, Renata and Mitrović, Slobodanka and Jeftić, Ilija and Kanjevac, Tatjana and Lukić, Aleksandra",
year = "2016",
abstract = "BACKGROUND: Interleukin-33 (IL-33) is a recently identified cytokine belonging to the IL-1 family and ligand for the IL-1 receptor-related protein ST2. IL-33/ST2 signaling plays a critical role in allergy, autoimmunity, and chronic inflammatory disorders, but its role in the pathogenesis of periapical lesions is unknown. We aimed to investigate the expression patterns of IL-33 and ST2 in human periapical lesions. METHODS: Periapical lesions (n = 36) and healthy periapical tissues (n = 10) were evaluated by immunohistochemistry using antibodies specific for human IL-33 and ST2. Lesion samples were further analyzed by double immunofluorescence to assess IL-33/ST2 co-expression. RESULTS: The numbers of IL-33- and ST2-positive fibroblasts were significantly higher in periapical lesions compared to healthy periapical tissues (both P  lt  0.05), while the numbers of IL-33-and ST2-positive endothelial cells were similar (both P > 0.05). There were no significant differences in the numbers of IL-33-and ST2-positive fibroblasts and endothelial cells between periapical granulomas and radicular cysts (all P > 0.05). Similarly, numbers of ST2-positive mononuclear cells did not differ between periapical granulomas and radicular cysts (P > 0.05). The majority of epithelial cells in radicular cysts were IL-33 positive, while the small proportion of epithelial cells was ST2 positive. Double immunofluorescence analysis revealed IL-33/ST2 co-expression in fibroblasts and endothelial cells. CONCLUSIONS: IL-33 and ST2 are expressed in periapical granulomas and radicular cysts. Increased numbers of IL-33-and ST2-positive fibroblasts in periapical lesions when compared to healthy periapical tissues suggest that IL-33/ST2 signaling may be involved in periapical inflammation and tissue fibrosis.",
publisher = "Wiley, Hoboken",
journal = "Journal of Oral Pathology & Medicine",
title = "Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts",
volume = "45",
number = "1",
pages = "70-76",
doi = "10.1111/jop.12312"
}

Velicković, M., Pejnović, N., Petrović, R., Mitrović, S., Jeftić, I., Kanjevac, T.,& Lukić, A.. (2016). Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts. in Journal of Oral Pathology & Medicine
Wiley, Hoboken., 45(1), 70-76.
https://doi.org/10.1111/jop.12312

Velicković M, Pejnović N, Petrović R, Mitrović S, Jeftić I, Kanjevac T, Lukić A. Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts. in Journal of Oral Pathology & Medicine. 2016;45(1):70-76.
doi:10.1111/jop.12312 .

Velicković, Milena, Pejnović, Nada, Petrović, Renata, Mitrović, Slobodanka, Jeftić, Ilija, Kanjevac, Tatjana, Lukić, Aleksandra, "Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts" in Journal of Oral Pathology & Medicine, 45, no. 1 (2016):70-76,
https://doi.org/10.1111/jop.12312 . .

TY  - JOUR
AU  - Kanjevac, Tatjana
AU  - Milovanović, Marija
AU  - Milošević-Đorđević, Olivera
AU  - Tešić, Živoslav
AU  - Ivanović, Mirjana
AU  - Lukić, Aleksandra
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1968
AB  - Stem cells from human exfoliated deciduous teeth (SHED) can be used as a cell-based therapy in regenerative medicine and in immunomodulation. Pulp from human deciduous teeth can be stored as a source of SHED. Glass ionomer cements (GICs) are commonly used in restorative dentistry and in cavity lining. GICs have lower biocompatibility and are cytotoxic for dental pulp cells. In this study, seven commonly used GICs were tested for their cytotoxic effects on SHED, for their potential to arrest mitosis in cells and induce chromosome aberrations, and were compared with the effects of composite. Fuji II, Fuji VIII, Fuji IX, Fuji plus and Vitrebond had significantly higher cytotoxic effects on SHED than composite. Only SHEDs that have been treated with Fuji I, Fuji IX, Fuji plus and composite recovered the potential for proliferation, but no chromosome aberrations were found after treatment with GICs. The cytotoxic effects of GICs on SHEDs were in strong correlation with combined concentrations of released fluoride, aluminum and strontium ions. Fuji I exhibited the lowest activity towards SHEDs; it did not interrupt mitosis and did not induce chromosome aberrations, and was accompanied by the lowest levels of released F, Al and Sr ions.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrations
VL  - 67
IS  - 2
SP  - 619
EP  - 630
DO  - 10.2298/ABS141021022K
ER  - 

@article{
author = "Kanjevac, Tatjana and Milovanović, Marija and Milošević-Đorđević, Olivera and Tešić, Živoslav and Ivanović, Mirjana and Lukić, Aleksandra",
year = "2015",
abstract = "Stem cells from human exfoliated deciduous teeth (SHED) can be used as a cell-based therapy in regenerative medicine and in immunomodulation. Pulp from human deciduous teeth can be stored as a source of SHED. Glass ionomer cements (GICs) are commonly used in restorative dentistry and in cavity lining. GICs have lower biocompatibility and are cytotoxic for dental pulp cells. In this study, seven commonly used GICs were tested for their cytotoxic effects on SHED, for their potential to arrest mitosis in cells and induce chromosome aberrations, and were compared with the effects of composite. Fuji II, Fuji VIII, Fuji IX, Fuji plus and Vitrebond had significantly higher cytotoxic effects on SHED than composite. Only SHEDs that have been treated with Fuji I, Fuji IX, Fuji plus and composite recovered the potential for proliferation, but no chromosome aberrations were found after treatment with GICs. The cytotoxic effects of GICs on SHEDs were in strong correlation with combined concentrations of released fluoride, aluminum and strontium ions. Fuji I exhibited the lowest activity towards SHEDs; it did not interrupt mitosis and did not induce chromosome aberrations, and was accompanied by the lowest levels of released F, Al and Sr ions.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrations",
volume = "67",
number = "2",
pages = "619-630",
doi = "10.2298/ABS141021022K"
}

Kanjevac, T., Milovanović, M., Milošević-Đorđević, O., Tešić, Ž., Ivanović, M.,& Lukić, A.. (2015). Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrations. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 67(2), 619-630.
https://doi.org/10.2298/ABS141021022K

Kanjevac T, Milovanović M, Milošević-Đorđević O, Tešić Ž, Ivanović M, Lukić A. Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrations. in Archives of Biological Sciences. 2015;67(2):619-630.
doi:10.2298/ABS141021022K .

Kanjevac, Tatjana, Milovanović, Marija, Milošević-Đorđević, Olivera, Tešić, Živoslav, Ivanović, Mirjana, Lukić, Aleksandra, "Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrations" in Archives of Biological Sciences, 67, no. 2 (2015):619-630,
https://doi.org/10.2298/ABS141021022K . .

TY  - JOUR
AU  - Kanjevac, Tatjana
AU  - Milovanović, Marija
AU  - Volarević, Vladislav
AU  - Lukić, Miodrag L.
AU  - Arsenijević, Nebojša
AU  - Marković, Dejan
AU  - Zdravković, Nebojša
AU  - Tešić, Živoslav
AU  - Lukić, Aleksandra
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1752
AB  - Objectives: Glass ionomer cements (GICs) are commonly used as restorative materials. Responses to GICs differ among cell types and it is therefore of importance to thoroughly investigate the influence of these restorative materials on pulp stem cells that are potential source for dental tissue regeneration. Eight biomaterials were tested: Fuji I, Fuji II, Fuji VIII, Fuji IX, Fuji Plus, Fuji Triage, Vitrebond and Composit. We compared their cytotoxic activity on human dental pulp stem cells (DPSC) and correlated this activity with the content of Fluoride, Aluminium and Strontium ions in their eluates. Methods: Elution samples of biomaterials were prepared in sterile tissue culture medium and the medium was tested for toxicity by an assay of cell survival/proliferation (MTT test) and apoptosis (Annexin V FITC Detection Kit). Concentrations of Fluoride, Aluminium and Strontium ions were tested by appropriate methods in the same eluates. Results: Cell survival ranged between 79.62% (Fuji Triage) to 1.5% (Fuji Plus) and most dead DPSCs were in the stage of late apoptosis. Fluoride release correlated with cytotoxicity of GICs, while Aluminium and Strontium ions, present in significant amount in eluates of tested GICs did not. Significance: Fuji Plus, Vitrebond and Fuji VIII, which released fluoride in higher quantities than other GICs, were highly toxic to human DPSCs. Opposite, low levels of released fluoride correlated to low cytotoxic effect of Composit, Fuji I and Fuji Triage.
PB  - Bentham Science Publ Ltd, Sharjah
T2  - Medicinal Chemistry
T1  - Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release
VL  - 8
IS  - 1
SP  - 40
EP  - 45
DO  - 10.2174/157340612799278351
ER  - 

@article{
author = "Kanjevac, Tatjana and Milovanović, Marija and Volarević, Vladislav and Lukić, Miodrag L. and Arsenijević, Nebojša and Marković, Dejan and Zdravković, Nebojša and Tešić, Živoslav and Lukić, Aleksandra",
year = "2012",
abstract = "Objectives: Glass ionomer cements (GICs) are commonly used as restorative materials. Responses to GICs differ among cell types and it is therefore of importance to thoroughly investigate the influence of these restorative materials on pulp stem cells that are potential source for dental tissue regeneration. Eight biomaterials were tested: Fuji I, Fuji II, Fuji VIII, Fuji IX, Fuji Plus, Fuji Triage, Vitrebond and Composit. We compared their cytotoxic activity on human dental pulp stem cells (DPSC) and correlated this activity with the content of Fluoride, Aluminium and Strontium ions in their eluates. Methods: Elution samples of biomaterials were prepared in sterile tissue culture medium and the medium was tested for toxicity by an assay of cell survival/proliferation (MTT test) and apoptosis (Annexin V FITC Detection Kit). Concentrations of Fluoride, Aluminium and Strontium ions were tested by appropriate methods in the same eluates. Results: Cell survival ranged between 79.62% (Fuji Triage) to 1.5% (Fuji Plus) and most dead DPSCs were in the stage of late apoptosis. Fluoride release correlated with cytotoxicity of GICs, while Aluminium and Strontium ions, present in significant amount in eluates of tested GICs did not. Significance: Fuji Plus, Vitrebond and Fuji VIII, which released fluoride in higher quantities than other GICs, were highly toxic to human DPSCs. Opposite, low levels of released fluoride correlated to low cytotoxic effect of Composit, Fuji I and Fuji Triage.",
publisher = "Bentham Science Publ Ltd, Sharjah",
journal = "Medicinal Chemistry",
title = "Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release",
volume = "8",
number = "1",
pages = "40-45",
doi = "10.2174/157340612799278351"
}

Kanjevac, T., Milovanović, M., Volarević, V., Lukić, M. L., Arsenijević, N., Marković, D., Zdravković, N., Tešić, Ž.,& Lukić, A.. (2012). Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release. in Medicinal Chemistry
Bentham Science Publ Ltd, Sharjah., 8(1), 40-45.
https://doi.org/10.2174/157340612799278351

Kanjevac T, Milovanović M, Volarević V, Lukić ML, Arsenijević N, Marković D, Zdravković N, Tešić Ž, Lukić A. Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release. in Medicinal Chemistry. 2012;8(1):40-45.
doi:10.2174/157340612799278351 .

Kanjevac, Tatjana, Milovanović, Marija, Volarević, Vladislav, Lukić, Miodrag L., Arsenijević, Nebojša, Marković, Dejan, Zdravković, Nebojša, Tešić, Živoslav, Lukić, Aleksandra, "Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release" in Medicinal Chemistry, 8, no. 1 (2012):40-45,
https://doi.org/10.2174/157340612799278351 . .

TY  - JOUR
AU  - Lukić, A.
AU  - Vasilijić, Saša
AU  - Majstorović, I.
AU  - Vucević, D.
AU  - Mojsilović, S.
AU  - Gazivoda, Dragan
AU  - Danilović, Vesna
AU  - Petrović, R.
AU  - Colić, M.
PY  - 2006
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1297
AB  - Aim To analyse phenotypic characteristics of antigen-presenting cells (APC), isolated from human periapical lesions by flow cytometry and immunocytochemistry. Methodology Sixteen periapical lesions were digested for 15 min with 0.05% collagenase. Mononuclear cells, separated from other inflammatory cells by density centrifugation, were processed for flow cytometry and/or immunocytochemistry. Single and double immunostainings were performed using monoclonal antibodies specific for human CD45, CD3, CD19, CD14, HLA-DR, CD1a, CD83 and CD123. Results Antigen-presenting cells (HLA-DR+ cells) represented 32.9 +/- 17.8% of total mononuclear cells. Amongst them, B cells (HLA-DR+ CD19(+)) were the predominant APC population, followed by activated macrophages (HLA-DR+ CD14(+)), dendritic cells (DC) (HLA-DR+ CD14(-) CD19(-) CD3(-)) and activated T cells (HLA-DR+ CD3(+)). Based on the predominance of T cells (CD3(+)) or B cells and plasma cells (CD19(+) and CD19(lo), respectively) amongst mononuclear cell infiltrates, lesions were divided into T- and B-types. The percentage of DC in T-type lesions (27.1 +/- 6.8% of total HLA-DR+ cells) was higher, compared with B-type lesions (10.3 +/- 5.2%) (P  lt  0.01). Within the DC population, the percentages of CD1a (Langerhans cell type) and CD123 (probably plasmacytoid DC type) did not differ significantly between the groups (P > 0.05). However, the percentage of mature DC (CD83(+)) was significantly higher in T-type periapical lesions (P  lt  0.05). Conclusion Flow cytometry and immunocytochemistry are suitable methods for phenotypic analysis of APC after their isolation from human periapical lesions. APC, that were phenotypically heterogeneous, constituted a significant component of infiltrating cells. Lesions with the predominance of T cells were characterized by a higher proportion of mature DC (HLA-DR(+)CD83(+) cells) than lesions with predominance of B cells/plasma cells.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Characterization of antigen-presenting cells in human apical periodontitis lesions by flow cytometry and immunocytochemistry
VL  - 39
IS  - 8
SP  - 626
EP  - 636
DO  - 10.1111/j.1365-2591.2006.01125.x
ER  - 

@article{
author = "Lukić, A. and Vasilijić, Saša and Majstorović, I. and Vucević, D. and Mojsilović, S. and Gazivoda, Dragan and Danilović, Vesna and Petrović, R. and Colić, M.",
year = "2006",
abstract = "Aim To analyse phenotypic characteristics of antigen-presenting cells (APC), isolated from human periapical lesions by flow cytometry and immunocytochemistry. Methodology Sixteen periapical lesions were digested for 15 min with 0.05% collagenase. Mononuclear cells, separated from other inflammatory cells by density centrifugation, were processed for flow cytometry and/or immunocytochemistry. Single and double immunostainings were performed using monoclonal antibodies specific for human CD45, CD3, CD19, CD14, HLA-DR, CD1a, CD83 and CD123. Results Antigen-presenting cells (HLA-DR+ cells) represented 32.9 +/- 17.8% of total mononuclear cells. Amongst them, B cells (HLA-DR+ CD19(+)) were the predominant APC population, followed by activated macrophages (HLA-DR+ CD14(+)), dendritic cells (DC) (HLA-DR+ CD14(-) CD19(-) CD3(-)) and activated T cells (HLA-DR+ CD3(+)). Based on the predominance of T cells (CD3(+)) or B cells and plasma cells (CD19(+) and CD19(lo), respectively) amongst mononuclear cell infiltrates, lesions were divided into T- and B-types. The percentage of DC in T-type lesions (27.1 +/- 6.8% of total HLA-DR+ cells) was higher, compared with B-type lesions (10.3 +/- 5.2%) (P  lt  0.01). Within the DC population, the percentages of CD1a (Langerhans cell type) and CD123 (probably plasmacytoid DC type) did not differ significantly between the groups (P > 0.05). However, the percentage of mature DC (CD83(+)) was significantly higher in T-type periapical lesions (P  lt  0.05). Conclusion Flow cytometry and immunocytochemistry are suitable methods for phenotypic analysis of APC after their isolation from human periapical lesions. APC, that were phenotypically heterogeneous, constituted a significant component of infiltrating cells. Lesions with the predominance of T cells were characterized by a higher proportion of mature DC (HLA-DR(+)CD83(+) cells) than lesions with predominance of B cells/plasma cells.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Characterization of antigen-presenting cells in human apical periodontitis lesions by flow cytometry and immunocytochemistry",
volume = "39",
number = "8",
pages = "626-636",
doi = "10.1111/j.1365-2591.2006.01125.x"
}

Lukić, A., Vasilijić, S., Majstorović, I., Vucević, D., Mojsilović, S., Gazivoda, D., Danilović, V., Petrović, R.,& Colić, M.. (2006). Characterization of antigen-presenting cells in human apical periodontitis lesions by flow cytometry and immunocytochemistry. in International Endodontic Journal
Wiley, Hoboken., 39(8), 626-636.
https://doi.org/10.1111/j.1365-2591.2006.01125.x

Lukić A, Vasilijić S, Majstorović I, Vucević D, Mojsilović S, Gazivoda D, Danilović V, Petrović R, Colić M. Characterization of antigen-presenting cells in human apical periodontitis lesions by flow cytometry and immunocytochemistry. in International Endodontic Journal. 2006;39(8):626-636.
doi:10.1111/j.1365-2591.2006.01125.x .

Lukić, A., Vasilijić, Saša, Majstorović, I., Vucević, D., Mojsilović, S., Gazivoda, Dragan, Danilović, Vesna, Petrović, R., Colić, M., "Characterization of antigen-presenting cells in human apical periodontitis lesions by flow cytometry and immunocytochemistry" in International Endodontic Journal, 39, no. 8 (2006):626-636,
https://doi.org/10.1111/j.1365-2591.2006.01125.x . .