TY  - JOUR
AU  - Mitić, Aleksandar
AU  - Popović, Jelena
AU  - Živković, Slavoljub
AU  - Mitić, Vladimir
AU  - Nikolić, Marija
AU  - Dačić, Stefan
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2153
AB  - Laser irradiation during root canal treatment is efficient but in order to prevent irreversible thermal damage to dentine, cementum and other periapical structures continuous water cooling is required. The aim of this study was to ultrastructurally analyze the surface of intracanal dentine and cementum at the root apex after Er:YAG laser irradiation with and without sterile saline solution irrigation. The study involved 70 intact maxillary and mandibular single-rooted and tworooted human teeth, divided into two experimental groups and one control group. In all teeth access cavity was created. For root canal irradiation, Er:YAG laser with the wavelength of 2940 nm, energy output of 140 mJ per pulse, pulse duration of 125-160 ms, pulse repetition rate of 10 Hz and power of 15 W was used. Total irradiation time for each root canal was 10 seconds. The ultrastructural analysis of intracanal dentine and the cementum of the root apex was done by using scanning electron microscope (JEOL JSM-5300 Tokyo Japan). Intracanal Er:YAG laser irradiation without water cooling showed melted circumpulpal dentine with large cracks in it, whereas cementum at the root apex was thermally modified in the form of plates and cracks. Intracanal Er:YAG laser irradiation with continuous water cooling resulted in clean intracanal dentine surface without morphological changes of the cementum. The canal system irradiation is acceptable and possible only when using constant water cooling; otherwise, irreversible thermal damage to intracanal dentine and cementum at the root apex occur.
AB  - Obrada kanala korena zuba laserom je efikasna, ali zahteva obavezno hlađenje vodom kako bi se sprečila ireverzibilna termička oštećenja dentina, cementa i ostalih periapeksnih struktura. Cilj ovog rada bio je da se ultrastrukturno analizira površina intrakanalnog dentina i cementnog tkiva na apeksu korena zuba posle obrade kanala Er:YAG laserom bez irigacije i sa irigacijom sterilnim fiziološkim rastvorom. Korišćeno je 70 intaktnih maksilarnih i mandibularnih jednokorenih i dvokorenih humanih zuba, podeljenih u dve eksperimentalne i jednu kontrolnu grupu. Kod svih zuba je formiran pristupni kavitet. Za obradu kanala korišćen je Er:YAG laser talasne dužine 2940 nm, izlazne energije za impuls 140 mJ; u pulsnom režimu 125-160ms, stopom pulsne repeticije od 10 Hz i energijom od 15 W. Vreme obrade svakog kanala laserom iznosilo je 10 sekundi. Ultrastrukturna analiza intrakanalnog dentina i cementnog tkiva na apeksnom delu korena zuba urađena je na skening elektronskom mikroskopu (JEOL JSM -5300 Tokyo Japan). Intrakanalna obrada Er:YAG laserom bez vodenog hlađenja pokazuje stopljen cirkumpulparni dentin sa velikim pukotinama u dentinu, a cementno tkivo na apeksu je termički modifikovano u vidu ploča i pukotina. Intrakanalna obrada Er:YAG laserom uz kontinuirano vodeno hlađenje daje čistu intrakanalnu dentinsku površinu bez morfoloških promena cementnog tkiva. Obrada kanalnog sistema zuba je opravdana i moguća samo u uslovima stalnog hlađenja vodom, u protivnom nastaju ireverzibilna termička oštećenja intrakanalnog dentina i cementa na apeksu korena zuba.
PB  - Univerzitet u Nišu - Medicinski fakultet, Niš
T2  - Acta medica Medianae
T1  - Effects of laser treatment on intracanal dentine and cementum at the root apex
T1  - Efekti lasero-terapije na intrakanalni dentin i cementno tkivo apeksa korena zuba
VL  - 55
IS  - 1
SP  - 26
EP  - 32
DO  - 10.5633/amm.2016.0104
ER  - 

@article{
author = "Mitić, Aleksandar and Popović, Jelena and Živković, Slavoljub and Mitić, Vladimir and Nikolić, Marija and Dačić, Stefan",
year = "2016",
abstract = "Laser irradiation during root canal treatment is efficient but in order to prevent irreversible thermal damage to dentine, cementum and other periapical structures continuous water cooling is required. The aim of this study was to ultrastructurally analyze the surface of intracanal dentine and cementum at the root apex after Er:YAG laser irradiation with and without sterile saline solution irrigation. The study involved 70 intact maxillary and mandibular single-rooted and tworooted human teeth, divided into two experimental groups and one control group. In all teeth access cavity was created. For root canal irradiation, Er:YAG laser with the wavelength of 2940 nm, energy output of 140 mJ per pulse, pulse duration of 125-160 ms, pulse repetition rate of 10 Hz and power of 15 W was used. Total irradiation time for each root canal was 10 seconds. The ultrastructural analysis of intracanal dentine and the cementum of the root apex was done by using scanning electron microscope (JEOL JSM-5300 Tokyo Japan). Intracanal Er:YAG laser irradiation without water cooling showed melted circumpulpal dentine with large cracks in it, whereas cementum at the root apex was thermally modified in the form of plates and cracks. Intracanal Er:YAG laser irradiation with continuous water cooling resulted in clean intracanal dentine surface without morphological changes of the cementum. The canal system irradiation is acceptable and possible only when using constant water cooling; otherwise, irreversible thermal damage to intracanal dentine and cementum at the root apex occur., Obrada kanala korena zuba laserom je efikasna, ali zahteva obavezno hlađenje vodom kako bi se sprečila ireverzibilna termička oštećenja dentina, cementa i ostalih periapeksnih struktura. Cilj ovog rada bio je da se ultrastrukturno analizira površina intrakanalnog dentina i cementnog tkiva na apeksu korena zuba posle obrade kanala Er:YAG laserom bez irigacije i sa irigacijom sterilnim fiziološkim rastvorom. Korišćeno je 70 intaktnih maksilarnih i mandibularnih jednokorenih i dvokorenih humanih zuba, podeljenih u dve eksperimentalne i jednu kontrolnu grupu. Kod svih zuba je formiran pristupni kavitet. Za obradu kanala korišćen je Er:YAG laser talasne dužine 2940 nm, izlazne energije za impuls 140 mJ; u pulsnom režimu 125-160ms, stopom pulsne repeticije od 10 Hz i energijom od 15 W. Vreme obrade svakog kanala laserom iznosilo je 10 sekundi. Ultrastrukturna analiza intrakanalnog dentina i cementnog tkiva na apeksnom delu korena zuba urađena je na skening elektronskom mikroskopu (JEOL JSM -5300 Tokyo Japan). Intrakanalna obrada Er:YAG laserom bez vodenog hlađenja pokazuje stopljen cirkumpulparni dentin sa velikim pukotinama u dentinu, a cementno tkivo na apeksu je termički modifikovano u vidu ploča i pukotina. Intrakanalna obrada Er:YAG laserom uz kontinuirano vodeno hlađenje daje čistu intrakanalnu dentinsku površinu bez morfoloških promena cementnog tkiva. Obrada kanalnog sistema zuba je opravdana i moguća samo u uslovima stalnog hlađenja vodom, u protivnom nastaju ireverzibilna termička oštećenja intrakanalnog dentina i cementa na apeksu korena zuba.",
publisher = "Univerzitet u Nišu - Medicinski fakultet, Niš",
journal = "Acta medica Medianae",
title = "Effects of laser treatment on intracanal dentine and cementum at the root apex, Efekti lasero-terapije na intrakanalni dentin i cementno tkivo apeksa korena zuba",
volume = "55",
number = "1",
pages = "26-32",
doi = "10.5633/amm.2016.0104"
}

Mitić, A., Popović, J., Živković, S., Mitić, V., Nikolić, M.,& Dačić, S.. (2016). Effects of laser treatment on intracanal dentine and cementum at the root apex. in Acta medica Medianae
Univerzitet u Nišu - Medicinski fakultet, Niš., 55(1), 26-32.
https://doi.org/10.5633/amm.2016.0104

Mitić A, Popović J, Živković S, Mitić V, Nikolić M, Dačić S. Effects of laser treatment on intracanal dentine and cementum at the root apex. in Acta medica Medianae. 2016;55(1):26-32.
doi:10.5633/amm.2016.0104 .

Mitić, Aleksandar, Popović, Jelena, Živković, Slavoljub, Mitić, Vladimir, Nikolić, Marija, Dačić, Stefan, "Effects of laser treatment on intracanal dentine and cementum at the root apex" in Acta medica Medianae, 55, no. 1 (2016):26-32,
https://doi.org/10.5633/amm.2016.0104 . .

TY  - JOUR
AU  - Mitić, Aleksandar
AU  - Mitić, Nadica
AU  - Milašin, Jelena
AU  - Živković, Slavoljub
AU  - Gašić, Jovanka
AU  - Mitić, Vladimir
AU  - Popović, Jelena
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1795
AB  - Introduction. Clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic. Objective. The aim of the paper was to assess the presence of genomes of the most common microorganisms (Porphyromonas gingivalis, Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella intermedia, Treponema denticola and Enterococcus faecalis) in infected tooth root canals before and after rinsing with solution of doxycycline, citric acid and detergent Tween-80 (MTAD) in patients with clinically diagnosed primary apex periodontitis. Methods. The content of primarily infected canals before and after using the MTAD solution was used as a biological material in which the presence of microorganisms DNA was proved. For the detection of bacterial genome the multiplex PCR technique was applied. Results. The percentage of positive samples before canal treatment was 100%. In infected root canals E. faecalis was most dominant (37%). In a relatively high percentage we detected P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) and P. gingivalis (10%). After rinsing the canal system using MTAD solution, there was a statistically significant decrease in E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) and P. gingivalis (0%). The presence of other bacteria was also diminished but not statistically significantly. Conclusion. With the application of multiplex PCR technique which provided a simultaneous amplification of various genomic sequences, using several pairs of primers, the most dominant in infected root canals were E. faecalis. P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis and P. gingivalis. After mechanic treatment and irrigation of root canals with MTAD solution, P. intermedia, P. gingivalis and T. forsythensis were not found. The presence of E. faecalis, A. actinomycetemcomitans and T. denticola was diminished, however, not statistically significantly.
AB  - Uvod. Klinički prihvatljiv intrakanalni antiseptik mora imati organolitičko-mineralolitičko dejstvo i antibakterijsku efikasnost i ne sme biti toksičan. Cilj rada. Cilj rada je bio da se ispita postojanje genoma najčešćih mikroorganizama (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythensis, Prevotella intermedia, Treponema denticola i Enterococcus faecalis) u inficiranim kanalima korena zuba pre i posle irigacije rastvorom doksiciklina, limunske kiseline i deterdženta Tween­80 (MTAD) kod osoba s klinički dijagnostikovanim primarnim apeksnim parodontitisom. Metode rada. Kao biološki materijal u kojem je dokazivano prisustvo DNK mikroorganizama korišćen je sadržaj iz primarno inficiranih kanala pre i posle irigacije rastvorom MTAD. Za otkrivanje bakterijskog genoma primenjena je multipleksna tehnika PCR. Rezultati. Procenat pozitivnih uzoraka pre obrade kanala bio je 100%. U inficiranim kanalima korena zuba najčešći je bio E. faecalis (37%). U relativno visokom procentu otkriveni su i: P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) i P. gingivalis (10%). Posle irigacije kanalnog sistema rastvorom MTAD utvrđeno je statistički značajno smanjenje učestalosti E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) i P. gingivalis (0%). Učestalost ostalih bakterija takođe se smanjila, ali ne statistički značajno. Zaključak. Primenom multipleksne PCR tehnike, koja omogućava istovremenu amplifikaciju genskih sekvenci uz korišćenje dva para prajmera, u inficiranim kanalima korena zuba najčešće je utvrđen E. faecalis. U relativno visokom procentu otkrivene su P. intermedia, A. actinomycetem­comitans, T. denticola, T. forsythensis i P. gingivalis. Posle hemomehaničke obrade i irigacije kanala rastvorom MTAD nisu zabeležene P. intermedia, P. gingivalis i T. forsythensis, dok je učestalost E. faecalis, A. actinomycetemcomitans i T. denticola smanjena, ali ne statistički značajno.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique
T1  - Analiza antimikrobnog dejstva rastvora MTAD u inficiranom kanalnom sistemu korena zuba tehnikom PCR
VL  - 141
IS  - 3-4
SP  - 155
EP  - 162
DO  - 10.2298/SARH1304155M
ER  - 

@article{
author = "Mitić, Aleksandar and Mitić, Nadica and Milašin, Jelena and Živković, Slavoljub and Gašić, Jovanka and Mitić, Vladimir and Popović, Jelena",
year = "2013",
abstract = "Introduction. Clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic. Objective. The aim of the paper was to assess the presence of genomes of the most common microorganisms (Porphyromonas gingivalis, Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella intermedia, Treponema denticola and Enterococcus faecalis) in infected tooth root canals before and after rinsing with solution of doxycycline, citric acid and detergent Tween-80 (MTAD) in patients with clinically diagnosed primary apex periodontitis. Methods. The content of primarily infected canals before and after using the MTAD solution was used as a biological material in which the presence of microorganisms DNA was proved. For the detection of bacterial genome the multiplex PCR technique was applied. Results. The percentage of positive samples before canal treatment was 100%. In infected root canals E. faecalis was most dominant (37%). In a relatively high percentage we detected P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) and P. gingivalis (10%). After rinsing the canal system using MTAD solution, there was a statistically significant decrease in E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) and P. gingivalis (0%). The presence of other bacteria was also diminished but not statistically significantly. Conclusion. With the application of multiplex PCR technique which provided a simultaneous amplification of various genomic sequences, using several pairs of primers, the most dominant in infected root canals were E. faecalis. P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis and P. gingivalis. After mechanic treatment and irrigation of root canals with MTAD solution, P. intermedia, P. gingivalis and T. forsythensis were not found. The presence of E. faecalis, A. actinomycetemcomitans and T. denticola was diminished, however, not statistically significantly., Uvod. Klinički prihvatljiv intrakanalni antiseptik mora imati organolitičko-mineralolitičko dejstvo i antibakterijsku efikasnost i ne sme biti toksičan. Cilj rada. Cilj rada je bio da se ispita postojanje genoma najčešćih mikroorganizama (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythensis, Prevotella intermedia, Treponema denticola i Enterococcus faecalis) u inficiranim kanalima korena zuba pre i posle irigacije rastvorom doksiciklina, limunske kiseline i deterdženta Tween­80 (MTAD) kod osoba s klinički dijagnostikovanim primarnim apeksnim parodontitisom. Metode rada. Kao biološki materijal u kojem je dokazivano prisustvo DNK mikroorganizama korišćen je sadržaj iz primarno inficiranih kanala pre i posle irigacije rastvorom MTAD. Za otkrivanje bakterijskog genoma primenjena je multipleksna tehnika PCR. Rezultati. Procenat pozitivnih uzoraka pre obrade kanala bio je 100%. U inficiranim kanalima korena zuba najčešći je bio E. faecalis (37%). U relativno visokom procentu otkriveni su i: P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) i P. gingivalis (10%). Posle irigacije kanalnog sistema rastvorom MTAD utvrđeno je statistički značajno smanjenje učestalosti E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) i P. gingivalis (0%). Učestalost ostalih bakterija takođe se smanjila, ali ne statistički značajno. Zaključak. Primenom multipleksne PCR tehnike, koja omogućava istovremenu amplifikaciju genskih sekvenci uz korišćenje dva para prajmera, u inficiranim kanalima korena zuba najčešće je utvrđen E. faecalis. U relativno visokom procentu otkrivene su P. intermedia, A. actinomycetem­comitans, T. denticola, T. forsythensis i P. gingivalis. Posle hemomehaničke obrade i irigacije kanala rastvorom MTAD nisu zabeležene P. intermedia, P. gingivalis i T. forsythensis, dok je učestalost E. faecalis, A. actinomycetemcomitans i T. denticola smanjena, ali ne statistički značajno.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique, Analiza antimikrobnog dejstva rastvora MTAD u inficiranom kanalnom sistemu korena zuba tehnikom PCR",
volume = "141",
number = "3-4",
pages = "155-162",
doi = "10.2298/SARH1304155M"
}

Mitić, A., Mitić, N., Milašin, J., Živković, S., Gašić, J., Mitić, V.,& Popović, J.. (2013). Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 141(3-4), 155-162.
https://doi.org/10.2298/SARH1304155M

Mitić A, Mitić N, Milašin J, Živković S, Gašić J, Mitić V, Popović J. Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique. in Srpski arhiv za celokupno lekarstvo. 2013;141(3-4):155-162.
doi:10.2298/SARH1304155M .

Mitić, Aleksandar, Mitić, Nadica, Milašin, Jelena, Živković, Slavoljub, Gašić, Jovanka, Mitić, Vladimir, Popović, Jelena, "Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique" in Srpski arhiv za celokupno lekarstvo, 141, no. 3-4 (2013):155-162,
https://doi.org/10.2298/SARH1304155M . .