Dačić, Stefan

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Effects of laser treatment on intracanal dentine and cementum at the root apex

Mitić, Aleksandar; Popović, Jelena; Živković, Slavoljub; Mitić, Vladimir; Nikolić, Marija; Dačić, Stefan

(Univerzitet u Nišu - Medicinski fakultet, Niš, 2016)

TY  - JOUR
AU  - Mitić, Aleksandar
AU  - Popović, Jelena
AU  - Živković, Slavoljub
AU  - Mitić, Vladimir
AU  - Nikolić, Marija
AU  - Dačić, Stefan
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2153
AB  - Laser irradiation during root canal treatment is efficient but in order to prevent irreversible thermal damage to dentine, cementum and other periapical structures continuous water cooling is required. The aim of this study was to ultrastructurally analyze the surface of intracanal dentine and cementum at the root apex after Er:YAG laser irradiation with and without sterile saline solution irrigation. The study involved 70 intact maxillary and mandibular single-rooted and tworooted human teeth, divided into two experimental groups and one control group. In all teeth access cavity was created. For root canal irradiation, Er:YAG laser with the wavelength of 2940 nm, energy output of 140 mJ per pulse, pulse duration of 125-160 ms, pulse repetition rate of 10 Hz and power of 15 W was used. Total irradiation time for each root canal was 10 seconds. The ultrastructural analysis of intracanal dentine and the cementum of the root apex was done by using scanning electron microscope (JEOL JSM-5300 Tokyo Japan). Intracanal Er:YAG laser irradiation without water cooling showed melted circumpulpal dentine with large cracks in it, whereas cementum at the root apex was thermally modified in the form of plates and cracks. Intracanal Er:YAG laser irradiation with continuous water cooling resulted in clean intracanal dentine surface without morphological changes of the cementum. The canal system irradiation is acceptable and possible only when using constant water cooling; otherwise, irreversible thermal damage to intracanal dentine and cementum at the root apex occur.
AB  - Obrada kanala korena zuba laserom je efikasna, ali zahteva obavezno hlađenje vodom kako bi se sprečila ireverzibilna termička oštećenja dentina, cementa i ostalih periapeksnih struktura. Cilj ovog rada bio je da se ultrastrukturno analizira površina intrakanalnog dentina i cementnog tkiva na apeksu korena zuba posle obrade kanala Er:YAG laserom bez irigacije i sa irigacijom sterilnim fiziološkim rastvorom. Korišćeno je 70 intaktnih maksilarnih i mandibularnih jednokorenih i dvokorenih humanih zuba, podeljenih u dve eksperimentalne i jednu kontrolnu grupu. Kod svih zuba je formiran pristupni kavitet. Za obradu kanala korišćen je Er:YAG laser talasne dužine 2940 nm, izlazne energije za impuls 140 mJ; u pulsnom režimu 125-160ms, stopom pulsne repeticije od 10 Hz i energijom od 15 W. Vreme obrade svakog kanala laserom iznosilo je 10 sekundi. Ultrastrukturna analiza intrakanalnog dentina i cementnog tkiva na apeksnom delu korena zuba urađena je na skening elektronskom mikroskopu (JEOL JSM -5300 Tokyo Japan). Intrakanalna obrada Er:YAG laserom bez vodenog hlađenja pokazuje stopljen cirkumpulparni dentin sa velikim pukotinama u dentinu, a cementno tkivo na apeksu je termički modifikovano u vidu ploča i pukotina. Intrakanalna obrada Er:YAG laserom uz kontinuirano vodeno hlađenje daje čistu intrakanalnu dentinsku površinu bez morfoloških promena cementnog tkiva. Obrada kanalnog sistema zuba je opravdana i moguća samo u uslovima stalnog hlađenja vodom, u protivnom nastaju ireverzibilna termička oštećenja intrakanalnog dentina i cementa na apeksu korena zuba.
PB  - Univerzitet u Nišu - Medicinski fakultet, Niš
T2  - Acta medica Medianae
T1  - Effects of laser treatment on intracanal dentine and cementum at the root apex
T1  - Efekti lasero-terapije na intrakanalni dentin i cementno tkivo apeksa korena zuba
VL  - 55
IS  - 1
SP  - 26
EP  - 32
DO  - 10.5633/amm.2016.0104
ER  - 
@article{
author = "Mitić, Aleksandar and Popović, Jelena and Živković, Slavoljub and Mitić, Vladimir and Nikolić, Marija and Dačić, Stefan",
year = "2016",
abstract = "Laser irradiation during root canal treatment is efficient but in order to prevent irreversible thermal damage to dentine, cementum and other periapical structures continuous water cooling is required. The aim of this study was to ultrastructurally analyze the surface of intracanal dentine and cementum at the root apex after Er:YAG laser irradiation with and without sterile saline solution irrigation. The study involved 70 intact maxillary and mandibular single-rooted and tworooted human teeth, divided into two experimental groups and one control group. In all teeth access cavity was created. For root canal irradiation, Er:YAG laser with the wavelength of 2940 nm, energy output of 140 mJ per pulse, pulse duration of 125-160 ms, pulse repetition rate of 10 Hz and power of 15 W was used. Total irradiation time for each root canal was 10 seconds. The ultrastructural analysis of intracanal dentine and the cementum of the root apex was done by using scanning electron microscope (JEOL JSM-5300 Tokyo Japan). Intracanal Er:YAG laser irradiation without water cooling showed melted circumpulpal dentine with large cracks in it, whereas cementum at the root apex was thermally modified in the form of plates and cracks. Intracanal Er:YAG laser irradiation with continuous water cooling resulted in clean intracanal dentine surface without morphological changes of the cementum. The canal system irradiation is acceptable and possible only when using constant water cooling; otherwise, irreversible thermal damage to intracanal dentine and cementum at the root apex occur., Obrada kanala korena zuba laserom je efikasna, ali zahteva obavezno hlađenje vodom kako bi se sprečila ireverzibilna termička oštećenja dentina, cementa i ostalih periapeksnih struktura. Cilj ovog rada bio je da se ultrastrukturno analizira površina intrakanalnog dentina i cementnog tkiva na apeksu korena zuba posle obrade kanala Er:YAG laserom bez irigacije i sa irigacijom sterilnim fiziološkim rastvorom. Korišćeno je 70 intaktnih maksilarnih i mandibularnih jednokorenih i dvokorenih humanih zuba, podeljenih u dve eksperimentalne i jednu kontrolnu grupu. Kod svih zuba je formiran pristupni kavitet. Za obradu kanala korišćen je Er:YAG laser talasne dužine 2940 nm, izlazne energije za impuls 140 mJ; u pulsnom režimu 125-160ms, stopom pulsne repeticije od 10 Hz i energijom od 15 W. Vreme obrade svakog kanala laserom iznosilo je 10 sekundi. Ultrastrukturna analiza intrakanalnog dentina i cementnog tkiva na apeksnom delu korena zuba urađena je na skening elektronskom mikroskopu (JEOL JSM -5300 Tokyo Japan). Intrakanalna obrada Er:YAG laserom bez vodenog hlađenja pokazuje stopljen cirkumpulparni dentin sa velikim pukotinama u dentinu, a cementno tkivo na apeksu je termički modifikovano u vidu ploča i pukotina. Intrakanalna obrada Er:YAG laserom uz kontinuirano vodeno hlađenje daje čistu intrakanalnu dentinsku površinu bez morfoloških promena cementnog tkiva. Obrada kanalnog sistema zuba je opravdana i moguća samo u uslovima stalnog hlađenja vodom, u protivnom nastaju ireverzibilna termička oštećenja intrakanalnog dentina i cementa na apeksu korena zuba.",
publisher = "Univerzitet u Nišu - Medicinski fakultet, Niš",
journal = "Acta medica Medianae",
title = "Effects of laser treatment on intracanal dentine and cementum at the root apex, Efekti lasero-terapije na intrakanalni dentin i cementno tkivo apeksa korena zuba",
volume = "55",
number = "1",
pages = "26-32",
doi = "10.5633/amm.2016.0104"
}
Mitić, A., Popović, J., Živković, S., Mitić, V., Nikolić, M.,& Dačić, S.. (2016). Effects of laser treatment on intracanal dentine and cementum at the root apex. in Acta medica Medianae
Univerzitet u Nišu - Medicinski fakultet, Niš., 55(1), 26-32.
https://doi.org/10.5633/amm.2016.0104
Mitić A, Popović J, Živković S, Mitić V, Nikolić M, Dačić S. Effects of laser treatment on intracanal dentine and cementum at the root apex. in Acta medica Medianae. 2016;55(1):26-32.
doi:10.5633/amm.2016.0104 .
Mitić, Aleksandar, Popović, Jelena, Živković, Slavoljub, Mitić, Vladimir, Nikolić, Marija, Dačić, Stefan, "Effects of laser treatment on intracanal dentine and cementum at the root apex" in Acta medica Medianae, 55, no. 1 (2016):26-32,
https://doi.org/10.5633/amm.2016.0104 . .

Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization

Dačić, Stefan; Dačić-Simonović, Dragica; Živković, Slavoljub; Dačić, Miloš; Radičević, Goran; Mitić, Aleksandar; Tošić, Goran; Igić, Marko

(Srpsko lekarsko društvo, Beograd, 2014)

TY  - JOUR
AU  - Dačić, Stefan
AU  - Dačić-Simonović, Dragica
AU  - Živković, Slavoljub
AU  - Dačić, Miloš
AU  - Radičević, Goran
AU  - Mitić, Aleksandar
AU  - Tošić, Goran
AU  - Igić, Marko
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1914
AB  - Introduction Bonding between composite and hard dental tissue is most commonly assessed by measuring bonding strength or absence of marginal gap along the restoration interface. Marginal index (MI) is a significant indicator of the efficiency of the bond between material and dental tissue because it also shows the values of width and length of marginal gap. Objective The aim of this investigation was to estimate quantitative and qualitative features of the bond between composite resin and enamel and to determine the values of MI in enamel after application of two techniques of photopolymerization with two composite systems. Methods Forty Class V cavities on extracted teeth were prepared and restored for scanning electron microscope (SEM) analysis of composite bonding to enamel. Adhesion to enamel was achieved by Adper Single Bond 2 - ASB (3M ESPE), or by Adper Easy One - AEO (3M ESPE). Photopolymerization of Filtek Ultimate - FU (3M ESPE) was performed using constant halogen light (HIP) or soft start program (SOF). Results Quantitative and qualitative analysis, showed better mikromorphological bonding with SOF photopolymerization and ASB/FU composite system. Differences in MI between different photopolymerization techniques (HIP: 0.6707; SOF: 0.2395) were statistically significant (p lt 0.001), as well as differences between the composite systems (ASB/FU: 0.0470; AEO/ FU: 0.8651) (p lt 0.001) by two-way ANOVA test. Conclusion Better marginal adaptation of composite to enamel was obtained with SOF photopolymerization in both composite systems.
AB  - Uvod Kvalitet veze kompozita za tvrda zubna tkiva se najčešće procenjuje jačinom vezivanja ili izostankom marginalne pukotine po obodu restauracija. Marginalni indeks (MI) je važan pokazatelj efikasnosti pripoja materijala za zubna tkiva jer istovremeno pokazuje vrednosti i širine i dužine marginalne pukotine. Cilj rada Cilj rada je bio da se kvantitativno i kvalitativno proceni veza kompozitnih materijala za gleđ i utvrde vrednosti MI u gleđi nakon primene dve tehnike svetlosne polimerizacije i dva kompozitna sistema. Metode rada Na ekstrahovanim zubima je restaurisano 40 kaviteta pete klase za analizu pripoja kompozita za gleđ skening- elektronskom mikroskopijom (SEM). Adhezija za gleđ je obezbeđivana primenom Adper Single Bond 2 - ASB (3M ESPE), odnosno primenom Adper Easy One - AEO (3M ESPE). Svetlosna polimerizacija kompozitnog materijala (Filtek Ultimate - FU; 3M ESPE) vršena je standardnim halogenim svetlom (HIP) ili tzv. soft start programom (SOF). Rezultati Kvantitativna i kvalitativna analiza je pokazala bolje mikromorfološko vezivanje kompozita za gleđ kod SOF osvetljavanja i ASB/FU kompozitnog sistema. Na osnovu dvosmernog ANOVA testa, razlike u vrednosti MI između različitih svetlosnopolimerizujućih tehnika (HIP: 0,6707 i SOF: 0,2395) bile su statistički značajne (p lt 0,001), kao i razlike između kompozitnih sistema (ASB/FU: 0,0470 i AEO/FU: 0,8651; p lt 0,001). Zaključak Bolji marginalni pripoj kompozita za gleđ obezbeđen je primenom SOF osvetljavanja kod oba kompozitna sistema.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization
T1  - Analiza ivičnog pripoja kompozitnih smola za gleđ skening-elektronskom mikroskopijom nakon primene standardne i postepene tehnike fotopolimerizacije
VL  - 142
IS  - 7-8
SP  - 404
EP  - 412
DO  - 10.2298/SARH1408404D
ER  - 
@article{
author = "Dačić, Stefan and Dačić-Simonović, Dragica and Živković, Slavoljub and Dačić, Miloš and Radičević, Goran and Mitić, Aleksandar and Tošić, Goran and Igić, Marko",
year = "2014",
abstract = "Introduction Bonding between composite and hard dental tissue is most commonly assessed by measuring bonding strength or absence of marginal gap along the restoration interface. Marginal index (MI) is a significant indicator of the efficiency of the bond between material and dental tissue because it also shows the values of width and length of marginal gap. Objective The aim of this investigation was to estimate quantitative and qualitative features of the bond between composite resin and enamel and to determine the values of MI in enamel after application of two techniques of photopolymerization with two composite systems. Methods Forty Class V cavities on extracted teeth were prepared and restored for scanning electron microscope (SEM) analysis of composite bonding to enamel. Adhesion to enamel was achieved by Adper Single Bond 2 - ASB (3M ESPE), or by Adper Easy One - AEO (3M ESPE). Photopolymerization of Filtek Ultimate - FU (3M ESPE) was performed using constant halogen light (HIP) or soft start program (SOF). Results Quantitative and qualitative analysis, showed better mikromorphological bonding with SOF photopolymerization and ASB/FU composite system. Differences in MI between different photopolymerization techniques (HIP: 0.6707; SOF: 0.2395) were statistically significant (p lt 0.001), as well as differences between the composite systems (ASB/FU: 0.0470; AEO/ FU: 0.8651) (p lt 0.001) by two-way ANOVA test. Conclusion Better marginal adaptation of composite to enamel was obtained with SOF photopolymerization in both composite systems., Uvod Kvalitet veze kompozita za tvrda zubna tkiva se najčešće procenjuje jačinom vezivanja ili izostankom marginalne pukotine po obodu restauracija. Marginalni indeks (MI) je važan pokazatelj efikasnosti pripoja materijala za zubna tkiva jer istovremeno pokazuje vrednosti i širine i dužine marginalne pukotine. Cilj rada Cilj rada je bio da se kvantitativno i kvalitativno proceni veza kompozitnih materijala za gleđ i utvrde vrednosti MI u gleđi nakon primene dve tehnike svetlosne polimerizacije i dva kompozitna sistema. Metode rada Na ekstrahovanim zubima je restaurisano 40 kaviteta pete klase za analizu pripoja kompozita za gleđ skening- elektronskom mikroskopijom (SEM). Adhezija za gleđ je obezbeđivana primenom Adper Single Bond 2 - ASB (3M ESPE), odnosno primenom Adper Easy One - AEO (3M ESPE). Svetlosna polimerizacija kompozitnog materijala (Filtek Ultimate - FU; 3M ESPE) vršena je standardnim halogenim svetlom (HIP) ili tzv. soft start programom (SOF). Rezultati Kvantitativna i kvalitativna analiza je pokazala bolje mikromorfološko vezivanje kompozita za gleđ kod SOF osvetljavanja i ASB/FU kompozitnog sistema. Na osnovu dvosmernog ANOVA testa, razlike u vrednosti MI između različitih svetlosnopolimerizujućih tehnika (HIP: 0,6707 i SOF: 0,2395) bile su statistički značajne (p lt 0,001), kao i razlike između kompozitnih sistema (ASB/FU: 0,0470 i AEO/FU: 0,8651; p lt 0,001). Zaključak Bolji marginalni pripoj kompozita za gleđ obezbeđen je primenom SOF osvetljavanja kod oba kompozitna sistema.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization, Analiza ivičnog pripoja kompozitnih smola za gleđ skening-elektronskom mikroskopijom nakon primene standardne i postepene tehnike fotopolimerizacije",
volume = "142",
number = "7-8",
pages = "404-412",
doi = "10.2298/SARH1408404D"
}
Dačić, S., Dačić-Simonović, D., Živković, S., Dačić, M., Radičević, G., Mitić, A., Tošić, G.,& Igić, M.. (2014). Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 142(7-8), 404-412.
https://doi.org/10.2298/SARH1408404D
Dačić S, Dačić-Simonović D, Živković S, Dačić M, Radičević G, Mitić A, Tošić G, Igić M. Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization. in Srpski arhiv za celokupno lekarstvo. 2014;142(7-8):404-412.
doi:10.2298/SARH1408404D .
Dačić, Stefan, Dačić-Simonović, Dragica, Živković, Slavoljub, Dačić, Miloš, Radičević, Goran, Mitić, Aleksandar, Tošić, Goran, Igić, Marko, "Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization" in Srpski arhiv za celokupno lekarstvo, 142, no. 7-8 (2014):404-412,
https://doi.org/10.2298/SARH1408404D . .
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Efficiency of final irrigation of root canal in removal of smear layer

Mitić, Aleksandar; Mitić, Nadica; Živković, Slavoljub; Tošić, Goran; Savić, Vojin; Dačić, Stefan; Stojanović, Miodrag

(Srpsko lekarsko društvo, Beograd, 2009)

TY  - JOUR
AU  - Mitić, Aleksandar
AU  - Mitić, Nadica
AU  - Živković, Slavoljub
AU  - Tošić, Goran
AU  - Savić, Vojin
AU  - Dačić, Stefan
AU  - Stojanović, Miodrag
PY  - 2009
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1458
AB  - Introduction A smear layer forms on the root canal walls as the consequence of root instrumentation. The smear layer formed in such a way considerably influences the quality of root obturation and endodontic treatment outcome. Objective The aim of this study was to ultrastructurally analyze the surface of intracanal dentine after removal of the smear layer by the solution of doxycycline, citric acid and detergent Tween-80 (MTAD). Methods The study involved 60 single-rooted, extracted, human teeth divided into four groups. All samples were instrumented by a step-back technique and manual K-files, and rinsed during instrumentation by 2% CHX and H2O2. The first group of samples was exclusively rinsed by CHX and H2O2; in the second group, besides using CHX and H2O2, MTAD solution was used for the final irrigation. The samples which were rinsed by distilled water (+ control) and the samples rinsed by 5.25% NaOCl and 17% Na EDTA (-control) served as control groups. All samples were observed under the scanning electronic microscope JEOL-JSM-5300. The coronary, middle and apex thirds of the radix region were analyzed. Results The obtained results of the SEM analysis showed that the application of 2% chlorhexidine and hydrogen peroxide did not give clear dentine walls, and the smear layer could not be removed completely. The application of the same combination (CHX and H2O2), added by the final MTAD irrigation solution, led to the efficient removal of the smear layer, while the morphological structure of dentine surface remained unchanged. Statistical analysis showed that canal walls in the experimental group with MTAD as the final irrigation were significantly clearer compared to the control group (p lt 0.001). Conclusion Based on the obtained results, it can be concluded that MTAD is an efficient solution for the final irrigation of the canal system.
AB  - Uvod Razmazni sloj koji se stvara na zidovima kanala korena zuba posledica je instrumentacije kanala. On značajno utiče na kvalitet opturacije kanala i krajnji ishod endodontskog lečenja. Cilj rada Cilj istraživanja je bio da se ustanovi stepen efikasnosti rastvora doksiciklina, limunske kiseline i deterxenta Tween-80 (poznate pod nazivom MTAD) kao završnog irigansa u uklanjanju razmaznog sloja s površina intrakanalnog dentina. Metode rada Ispitano je 60 jednokorenih ekstrahovanih humanih zuba, podeljenih u četiri grupe. Svi uzorci su instrumentirani tehnikom step-back i ručnim K-turpijama, a tokom instrumentacije ispirani dvoprocentnim hlorheksidinom (CHX) i tropostotnim vodonik-peroksidom (H2O2). Prva grupa uzoraka je ispirana isključivo sa CHX i H2O2, dok je za završnu irigaciju uzoraka druge grupe korišćen i rastvor MTAD. Svi uzorci su posmatrani na skening elektronskom mikroskopu JEOL-JSM-5300. Ultrastrukturno su analizirane koronarna, srednja i apikalna trećina korenske regije. Rezultati Primenom samo CHX i H2O2 kao irigansa ne dobijaju se čisti dentinski zidovi, a razmazni sloj sa zidova nije potpuno uklonjen. Istom kombinacijom irigansa koja je dopunjena rastvorom MTAD za završno ispiranje razmazni sloj se efikasno uklanja, a morfološka struktura dentinske površine se ne menja. Statistička analiza je pokazala da su zidovi kanala uzoraka ispiranih i sa MTAD značajno čistiji nego kod uzoraka kontrolne grupe (p lt 0,001). Zaključak Na osnovu rezultata istraživanja može se zaključiti da je MTAD bio najefikasniji rastvor za završnu irigaciju kanalnog sistema korena zuba.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Efficiency of final irrigation of root canal in removal of smear layer
T1  - Efikasnost sredstava za završnu irigaciju kanala korena zuba u uklanjanju razmaznog sloja
VL  - 137
IS  - 9-10
SP  - 482
EP  - 489
DO  - 10.2298/SARH0910482M
ER  - 
@article{
author = "Mitić, Aleksandar and Mitić, Nadica and Živković, Slavoljub and Tošić, Goran and Savić, Vojin and Dačić, Stefan and Stojanović, Miodrag",
year = "2009",
abstract = "Introduction A smear layer forms on the root canal walls as the consequence of root instrumentation. The smear layer formed in such a way considerably influences the quality of root obturation and endodontic treatment outcome. Objective The aim of this study was to ultrastructurally analyze the surface of intracanal dentine after removal of the smear layer by the solution of doxycycline, citric acid and detergent Tween-80 (MTAD). Methods The study involved 60 single-rooted, extracted, human teeth divided into four groups. All samples were instrumented by a step-back technique and manual K-files, and rinsed during instrumentation by 2% CHX and H2O2. The first group of samples was exclusively rinsed by CHX and H2O2; in the second group, besides using CHX and H2O2, MTAD solution was used for the final irrigation. The samples which were rinsed by distilled water (+ control) and the samples rinsed by 5.25% NaOCl and 17% Na EDTA (-control) served as control groups. All samples were observed under the scanning electronic microscope JEOL-JSM-5300. The coronary, middle and apex thirds of the radix region were analyzed. Results The obtained results of the SEM analysis showed that the application of 2% chlorhexidine and hydrogen peroxide did not give clear dentine walls, and the smear layer could not be removed completely. The application of the same combination (CHX and H2O2), added by the final MTAD irrigation solution, led to the efficient removal of the smear layer, while the morphological structure of dentine surface remained unchanged. Statistical analysis showed that canal walls in the experimental group with MTAD as the final irrigation were significantly clearer compared to the control group (p lt 0.001). Conclusion Based on the obtained results, it can be concluded that MTAD is an efficient solution for the final irrigation of the canal system., Uvod Razmazni sloj koji se stvara na zidovima kanala korena zuba posledica je instrumentacije kanala. On značajno utiče na kvalitet opturacije kanala i krajnji ishod endodontskog lečenja. Cilj rada Cilj istraživanja je bio da se ustanovi stepen efikasnosti rastvora doksiciklina, limunske kiseline i deterxenta Tween-80 (poznate pod nazivom MTAD) kao završnog irigansa u uklanjanju razmaznog sloja s površina intrakanalnog dentina. Metode rada Ispitano je 60 jednokorenih ekstrahovanih humanih zuba, podeljenih u četiri grupe. Svi uzorci su instrumentirani tehnikom step-back i ručnim K-turpijama, a tokom instrumentacije ispirani dvoprocentnim hlorheksidinom (CHX) i tropostotnim vodonik-peroksidom (H2O2). Prva grupa uzoraka je ispirana isključivo sa CHX i H2O2, dok je za završnu irigaciju uzoraka druge grupe korišćen i rastvor MTAD. Svi uzorci su posmatrani na skening elektronskom mikroskopu JEOL-JSM-5300. Ultrastrukturno su analizirane koronarna, srednja i apikalna trećina korenske regije. Rezultati Primenom samo CHX i H2O2 kao irigansa ne dobijaju se čisti dentinski zidovi, a razmazni sloj sa zidova nije potpuno uklonjen. Istom kombinacijom irigansa koja je dopunjena rastvorom MTAD za završno ispiranje razmazni sloj se efikasno uklanja, a morfološka struktura dentinske površine se ne menja. Statistička analiza je pokazala da su zidovi kanala uzoraka ispiranih i sa MTAD značajno čistiji nego kod uzoraka kontrolne grupe (p lt 0,001). Zaključak Na osnovu rezultata istraživanja može se zaključiti da je MTAD bio najefikasniji rastvor za završnu irigaciju kanalnog sistema korena zuba.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Efficiency of final irrigation of root canal in removal of smear layer, Efikasnost sredstava za završnu irigaciju kanala korena zuba u uklanjanju razmaznog sloja",
volume = "137",
number = "9-10",
pages = "482-489",
doi = "10.2298/SARH0910482M"
}
Mitić, A., Mitić, N., Živković, S., Tošić, G., Savić, V., Dačić, S.,& Stojanović, M.. (2009). Efficiency of final irrigation of root canal in removal of smear layer. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 137(9-10), 482-489.
https://doi.org/10.2298/SARH0910482M
Mitić A, Mitić N, Živković S, Tošić G, Savić V, Dačić S, Stojanović M. Efficiency of final irrigation of root canal in removal of smear layer. in Srpski arhiv za celokupno lekarstvo. 2009;137(9-10):482-489.
doi:10.2298/SARH0910482M .
Mitić, Aleksandar, Mitić, Nadica, Živković, Slavoljub, Tošić, Goran, Savić, Vojin, Dačić, Stefan, Stojanović, Miodrag, "Efficiency of final irrigation of root canal in removal of smear layer" in Srpski arhiv za celokupno lekarstvo, 137, no. 9-10 (2009):482-489,
https://doi.org/10.2298/SARH0910482M . .
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SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system

Dačić, Stefan; Dačić-Simonović, Dragica; Živković, Slavoljub; Radičević, Goran; Mitić, Aleksandar; Stanojević, Ivana; Veselinović, Aleksandar

(Srpsko lekarsko društvo, Beograd, 2009)

TY  - JOUR
AU  - Dačić, Stefan
AU  - Dačić-Simonović, Dragica
AU  - Živković, Slavoljub
AU  - Radičević, Goran
AU  - Mitić, Aleksandar
AU  - Stanojević, Ivana
AU  - Veselinović, Aleksandar
PY  - 2009
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1457
AB  - Introduction Quality and durability of enamel/composite interface essentially depend on an adhesive restorative system. Objective The aim of this study was to evaluate the quality of marginal adaptation of composite adhesive systems to enamel by scanning electron microscopy (SEM), and to analyze the morphology of the enamel surface along margins of composite restorations, following exposure to cariogenic solution. Methods The study material included 56 extracted human third molars. Class V cavities were prepared with margins at enamel. Cavities were restored with Single Bond/Z250 and Single Bond/Filtek flow, using the total etch adhesive system, and Prompt-L-Pop/Z250 and Prompt-L-Pop/Filtek flow, using the self etch adhesive system. After the restorative procedure, the restorations were submitted to demineralization during 7 and 28 days. Samples were stored in a cariogenic solution (lactic acid, pH 4.5; 0.1 M) at 37°C or in deionized water (the control group). The margins of restorations and perimarginal enamel were examined by scanning electron microscope (SEM). The gap formations around restoration were measured on cervical, occlusal and approximate margins. Results The usage of the total etch adhesive system showed statistically significant lower marginal gap width around composite restoration, compared with the self etch system (p lt 0.01). The SEM examination also showed perimarginal enamel zones presenting several signs of demineralization and erosion (alteration of rods, porosities) after acting of a cariogenic solution, in both adhesive methods. Less alteration was found on the enamel surfaces not included in the perimarginal zone. Conclusion Treating the cavity with 35% phosphoric acid in the total etch system significantly improved the adaptation of the composite resins to enamel, compared with the self etch treatment. Stronger demineralization of the perimarginal enamel in a cariogenic solution was observed around all restorations in both adhesive systems.
AB  - Uvod Kvalitet i trajnost veze gleđi i kompozitnih materijala zavisi, između ostalog, i od korišćenog adhezivnog restaurativnog sistema. Cilj rada Cilj istraživanja je bio da se analizom pomoću skening elektronske mikroskopije (SEM) proceni kvalitet ivičnog pripoja kompozitnih adhezivnih sistema i gleđi, odnosno prouče morfološke osobine gleđne površine duž ivica kompozitnih restauracija nakon delovanja kariogenog rastvora. Metode rada Kao materijal u istraživanju korišćeno je 56 ekstrahovanih humanih trećih molara. Na svim zubima su preparirani kaviteti pete klase s ivicama u gleđi. Kaviteti su restaurirani kompozitnim adhezivnim sistemima: Single Bond/Z250 i Single Bond/Filtek Flow, korišćenjem tehnike totalnog nagrizanja, i Prompt-L-Pop/Z250 i Prompt- L-Pop/Filtek Flow, primenom tehnike sa samonagrizajućim adhezivnim sistemima. Posle postupka restauracije zubi su izlagani demineralizaciji tokom sedam i 28 dana potapanjem u kariogeni rastvor (mlečna kiselina pH 4,5; 0,1 M) na 37°C ili držani u dejonizovanoj vodi (kontrolna grupa). Ivice restauracija i perimarginalna gleđ ispitivane su pomoću SEM. Ivična pukotina oko restauracija merena je u predelu vrata zuba, okluzivno i na aproksimalnim stranama. Rezultati Primena tehnike totalnog nagrizanja je pokazala značajno manju širinu ivične pukotine oko kompozitnih restauracija u odnosu na samonagrizajući sistem (p lt 0,01). SEM ispitivanja su takođe otkrila izrazitu demineralizaciju i eroziju ivične zone gleđi (oštećenja prizmi, poroziteti) nakon delovanja kariogenog rastvora kod oba adhezivna sistema. Na gleđnim površinama van perimarginalne zone uočena su neznatna oštećenja. Zaključak Tretman kaviteta sa 35-procentnom fosfornom kiselinom kod totalno nagrizajućeg postupka značajno poboljšava vezu kompozitnih smola za gleđ. Rastvaranje ivične gleđi u kariogenom rastvoru uočeno je oko svih restauracija i kod oba adhezivna sistema.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system
T1  - SEM analiza kvaliteta ivičnog pripoja kompozitnih ispuna za gleđ posle primene totalno nagrizajućeg i samonagrizajućeg adhezivnog sistema
VL  - 137
IS  - 9-10
SP  - 475
EP  - 481
DO  - 10.2298/SARH0910475D
ER  - 
@article{
author = "Dačić, Stefan and Dačić-Simonović, Dragica and Živković, Slavoljub and Radičević, Goran and Mitić, Aleksandar and Stanojević, Ivana and Veselinović, Aleksandar",
year = "2009",
abstract = "Introduction Quality and durability of enamel/composite interface essentially depend on an adhesive restorative system. Objective The aim of this study was to evaluate the quality of marginal adaptation of composite adhesive systems to enamel by scanning electron microscopy (SEM), and to analyze the morphology of the enamel surface along margins of composite restorations, following exposure to cariogenic solution. Methods The study material included 56 extracted human third molars. Class V cavities were prepared with margins at enamel. Cavities were restored with Single Bond/Z250 and Single Bond/Filtek flow, using the total etch adhesive system, and Prompt-L-Pop/Z250 and Prompt-L-Pop/Filtek flow, using the self etch adhesive system. After the restorative procedure, the restorations were submitted to demineralization during 7 and 28 days. Samples were stored in a cariogenic solution (lactic acid, pH 4.5; 0.1 M) at 37°C or in deionized water (the control group). The margins of restorations and perimarginal enamel were examined by scanning electron microscope (SEM). The gap formations around restoration were measured on cervical, occlusal and approximate margins. Results The usage of the total etch adhesive system showed statistically significant lower marginal gap width around composite restoration, compared with the self etch system (p lt 0.01). The SEM examination also showed perimarginal enamel zones presenting several signs of demineralization and erosion (alteration of rods, porosities) after acting of a cariogenic solution, in both adhesive methods. Less alteration was found on the enamel surfaces not included in the perimarginal zone. Conclusion Treating the cavity with 35% phosphoric acid in the total etch system significantly improved the adaptation of the composite resins to enamel, compared with the self etch treatment. Stronger demineralization of the perimarginal enamel in a cariogenic solution was observed around all restorations in both adhesive systems., Uvod Kvalitet i trajnost veze gleđi i kompozitnih materijala zavisi, između ostalog, i od korišćenog adhezivnog restaurativnog sistema. Cilj rada Cilj istraživanja je bio da se analizom pomoću skening elektronske mikroskopije (SEM) proceni kvalitet ivičnog pripoja kompozitnih adhezivnih sistema i gleđi, odnosno prouče morfološke osobine gleđne površine duž ivica kompozitnih restauracija nakon delovanja kariogenog rastvora. Metode rada Kao materijal u istraživanju korišćeno je 56 ekstrahovanih humanih trećih molara. Na svim zubima su preparirani kaviteti pete klase s ivicama u gleđi. Kaviteti su restaurirani kompozitnim adhezivnim sistemima: Single Bond/Z250 i Single Bond/Filtek Flow, korišćenjem tehnike totalnog nagrizanja, i Prompt-L-Pop/Z250 i Prompt- L-Pop/Filtek Flow, primenom tehnike sa samonagrizajućim adhezivnim sistemima. Posle postupka restauracije zubi su izlagani demineralizaciji tokom sedam i 28 dana potapanjem u kariogeni rastvor (mlečna kiselina pH 4,5; 0,1 M) na 37°C ili držani u dejonizovanoj vodi (kontrolna grupa). Ivice restauracija i perimarginalna gleđ ispitivane su pomoću SEM. Ivična pukotina oko restauracija merena je u predelu vrata zuba, okluzivno i na aproksimalnim stranama. Rezultati Primena tehnike totalnog nagrizanja je pokazala značajno manju širinu ivične pukotine oko kompozitnih restauracija u odnosu na samonagrizajući sistem (p lt 0,01). SEM ispitivanja su takođe otkrila izrazitu demineralizaciju i eroziju ivične zone gleđi (oštećenja prizmi, poroziteti) nakon delovanja kariogenog rastvora kod oba adhezivna sistema. Na gleđnim površinama van perimarginalne zone uočena su neznatna oštećenja. Zaključak Tretman kaviteta sa 35-procentnom fosfornom kiselinom kod totalno nagrizajućeg postupka značajno poboljšava vezu kompozitnih smola za gleđ. Rastvaranje ivične gleđi u kariogenom rastvoru uočeno je oko svih restauracija i kod oba adhezivna sistema.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system, SEM analiza kvaliteta ivičnog pripoja kompozitnih ispuna za gleđ posle primene totalno nagrizajućeg i samonagrizajućeg adhezivnog sistema",
volume = "137",
number = "9-10",
pages = "475-481",
doi = "10.2298/SARH0910475D"
}
Dačić, S., Dačić-Simonović, D., Živković, S., Radičević, G., Mitić, A., Stanojević, I.,& Veselinović, A.. (2009). SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 137(9-10), 475-481.
https://doi.org/10.2298/SARH0910475D
Dačić S, Dačić-Simonović D, Živković S, Radičević G, Mitić A, Stanojević I, Veselinović A. SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system. in Srpski arhiv za celokupno lekarstvo. 2009;137(9-10):475-481.
doi:10.2298/SARH0910475D .
Dačić, Stefan, Dačić-Simonović, Dragica, Živković, Slavoljub, Radičević, Goran, Mitić, Aleksandar, Stanojević, Ivana, Veselinović, Aleksandar, "SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system" in Srpski arhiv za celokupno lekarstvo, 137, no. 9-10 (2009):475-481,
https://doi.org/10.2298/SARH0910475D . .
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