TY  - JOUR
AU  - Popović, Jelena
AU  - Radenković, Goran
AU  - Gašić, Jovanka
AU  - Živković, Slavoljub
AU  - Mitić, Aleksandar
AU  - Nikolić, Marija
AU  - Barac, Radomir
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2144
AB  - The application of irrigating solutions is essential in chemomechanical treatment of tooth root canal. However, chemical and electrochemical aggressiveness of the solutions, which directly act on the instruments, may damage their surface. The aim of this study was to investigate the sensitivity of the nickel-titanium (Ni-Ti) and stainless steel endodontic files to corrosive action of the sodium hypochlorite (NaOCl), chlorhexidine gluconate (CHX) and ethylene-diamine tetraacetic acid (EDTA). Testing of sensitivity to corrosion of the instruments was performed by dynamic potentiometric method. Measurements were made in 5.25% NaOCl, 0.2% CHX and 17% EDTA. Ni-Ti instruments immersed in 5.25% NaOCl showed the most intensive corrosive changes and the lowest value of pitting potential of 1.1 V. Stainless steel instruments immersed in 5.25% NaOCl showed higher value of pitting potential of 1.5 V. Stainless steel instruments immersed in 0.2% CHX showed lower corrosive surface changes and higher value of pitting potential of 1.6 V, whereas Ni-Ti instruments immersed in 0.2% CHX showed the pitting potential of 1.9 V. The corrosion was not observed in both types of instruments after immersion in 17% EDTA. The use of 5.25% NaOCl and 0.2% CHX may cause severe surface corrosion of Ni-Ti and stainless steel endodontic files.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly / CICEQ
T1  - The examination of sensitivity to corrosion of nickel-titanium and stainless steel endodontic instruments in tooth root canal irrigating solutions
VL  - 22
IS  - 1
SP  - 95
EP  - 100
DO  - 10.2298/CICEQ150103023P
ER  - 

@article{
author = "Popović, Jelena and Radenković, Goran and Gašić, Jovanka and Živković, Slavoljub and Mitić, Aleksandar and Nikolić, Marija and Barac, Radomir",
year = "2016",
abstract = "The application of irrigating solutions is essential in chemomechanical treatment of tooth root canal. However, chemical and electrochemical aggressiveness of the solutions, which directly act on the instruments, may damage their surface. The aim of this study was to investigate the sensitivity of the nickel-titanium (Ni-Ti) and stainless steel endodontic files to corrosive action of the sodium hypochlorite (NaOCl), chlorhexidine gluconate (CHX) and ethylene-diamine tetraacetic acid (EDTA). Testing of sensitivity to corrosion of the instruments was performed by dynamic potentiometric method. Measurements were made in 5.25% NaOCl, 0.2% CHX and 17% EDTA. Ni-Ti instruments immersed in 5.25% NaOCl showed the most intensive corrosive changes and the lowest value of pitting potential of 1.1 V. Stainless steel instruments immersed in 5.25% NaOCl showed higher value of pitting potential of 1.5 V. Stainless steel instruments immersed in 0.2% CHX showed lower corrosive surface changes and higher value of pitting potential of 1.6 V, whereas Ni-Ti instruments immersed in 0.2% CHX showed the pitting potential of 1.9 V. The corrosion was not observed in both types of instruments after immersion in 17% EDTA. The use of 5.25% NaOCl and 0.2% CHX may cause severe surface corrosion of Ni-Ti and stainless steel endodontic files.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly / CICEQ",
title = "The examination of sensitivity to corrosion of nickel-titanium and stainless steel endodontic instruments in tooth root canal irrigating solutions",
volume = "22",
number = "1",
pages = "95-100",
doi = "10.2298/CICEQ150103023P"
}

Popović, J., Radenković, G., Gašić, J., Živković, S., Mitić, A., Nikolić, M.,& Barac, R.. (2016). The examination of sensitivity to corrosion of nickel-titanium and stainless steel endodontic instruments in tooth root canal irrigating solutions. in Chemical Industry & Chemical Engineering Quarterly / CICEQ
Savez hemijskih inženjera, Beograd., 22(1), 95-100.
https://doi.org/10.2298/CICEQ150103023P

Popović J, Radenković G, Gašić J, Živković S, Mitić A, Nikolić M, Barac R. The examination of sensitivity to corrosion of nickel-titanium and stainless steel endodontic instruments in tooth root canal irrigating solutions. in Chemical Industry & Chemical Engineering Quarterly / CICEQ. 2016;22(1):95-100.
doi:10.2298/CICEQ150103023P .

Popović, Jelena, Radenković, Goran, Gašić, Jovanka, Živković, Slavoljub, Mitić, Aleksandar, Nikolić, Marija, Barac, Radomir, "The examination of sensitivity to corrosion of nickel-titanium and stainless steel endodontic instruments in tooth root canal irrigating solutions" in Chemical Industry & Chemical Engineering Quarterly / CICEQ, 22, no. 1 (2016):95-100,
https://doi.org/10.2298/CICEQ150103023P . .

TY  - JOUR
AU  - Barac, Radomir
AU  - Popović, Jelena
AU  - Živković, Slavoljub
AU  - Mitić, Aleksandar
AU  - Nikolić, Marija
AU  - Gašić, Jovanka
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2109
AB  - Introduction During endodontic treatment smaller or larger areas of root canal wall remain non-instrumented. This can affect prognosis of endodontic treatment as some bacteria may be left behind. The purpose of this study was to evaluate the morphology of non-instrumented areas of the root canal wall using scanning-electron-microscopy (SEM) after completed instrumentation and various irrigation regiments. Materials and Methods Eighteen single-rooted extracted teeth were divided into the six groups. One tooth in each group represented a control sample. In all samples only one half of the canal was instrumented using ISO 40 hand files. Control samples were subjected to an irrigation protocols without instrumentation. Irrigants used were physiological saline, 3% sodium hypochlorite and 15% of ethylene-diamine-tetra-acetate. Irrigation protocol included using each of these irrigants alone, or a combination of NaOCl and EDTA, as well as their combination with final irrigation using NaOCl or chlorhexidine. Then after, roots were sectioned longitudinally and prepared for SEM. Results Saline irrigation left pulpal debris on uninstrumented areas of the canal wall. Irrigation with 3% NaOCl left behind canal wall with different forms of calcospherites. However, after EDTA irrigation dentin appeared as an undulating surface with open tubules without a smear layer. The combination of NaOCl and EDTA showed remnants of calcospherites and open slightly widened dentinal tubules. Final irrigation with NaOCl on the uninstrumented areas showed enlarged dentinal tubules along with dentinal erosion, while after final irrigation with CHX clean dentin and open dentinal tubules without smear layer were noted. Conclusion From the morphological point of view, the most favorable effect of irrigation on both uninstrumented and uninstrumented canal walls was achieved after irrigation with NaOCl and EDTA or NaOCl, EDTA and chlorhexidine as the final irrigant.
AB  - Uvod Tokom endodontske terapije manje ili veće površine kanala korena zuba ostaju neinstrumentisane i mogu sadržavati bakterije, što može dovesti do neuspešnog ishoda. Cilj rada je bio da se skening-elekron-mikroskopskom (SEM) analizom proceni morfologija neistrumentisanih delova zidova kanala posle instrumentacije i primene različitih irigacionih protokola. Materijal i metode rada Osamnaest jednokorenih ekstrahovanih zuba je podeljeno u šest grupa. Jedan zub iz svake grupe je predstavljao kontrolni uzorak. Eksperimentalni uzorci su preparisani do instrumenta veličine ISO40. Kod svakog zuba je instrumentisana samo jedna polovina kanala. Kontrolni uzorci su podvrgnuti samo irigacionim protokolima bez preparacije. Osim fiziološkog rastvora, 3% natrijum-hipohlorita i 15% etilen-diamin-tetra-acetata, koji su primenjeni samostalno, eksperimentalni uzorci su irigirani i sa kombinacijom NaOCl i EDTA, kao i sa kombinacijom istih iriganasa, pri čemu je kao završni irigans korišćen ili NaOCl ili hlorheksidin. Korenovi su uzdužno presečeni i pripremljeni za SEM. Rezultati Posle irigacije sa fiziološkim rastvorom u neinstrumentisanim delovima kanala uočen je dentin pokriven debrisom, posle irigacije sa NaOCl dentin sa različitim oblicima kalcisferita. Posle irigacije sa EDTA uočena je talasasta površina dentinskog zida sa otvorenim tubulima bez razmaznog sloja. Irigacija kombinacijom NaOCl i EDTA je pokazala ostatke kalcisferita i otvorene, blago proširene dentinske tubule. Završno ispiranje sa NaOCl na neinstrumentisanim delovima dentina pokazuje proširene dentinske tubule i eroziju dentina, a završno ispiranje sa CHX čist dentin i otvorene dentinske tubule bez razmaznog sloja. Zaključak Sa morfološkog aspekta, najpovoljniji efekat irigacije (i kod instrumentisanih i kod neinstrumentisanih delova kanala) ustanovljen je posle irigacionih protokola sa: NaOCl i EDTA i NaOCl, EDTA i hlorheksidinom kao završnim irigansom.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Ultrastructural analysis of uninstrumented root canal areas following various irrigation regimens
T1  - Ultrastrukturna analiza neinstrumentisanih površina u kanalu korena posle različitih irigacionih protokola
VL  - 63
IS  - 2
SP  - 57
EP  - 65
DO  - 10.1515/sdj-2016-0006
ER  - 

@article{
author = "Barac, Radomir and Popović, Jelena and Živković, Slavoljub and Mitić, Aleksandar and Nikolić, Marija and Gašić, Jovanka",
year = "2016",
abstract = "Introduction During endodontic treatment smaller or larger areas of root canal wall remain non-instrumented. This can affect prognosis of endodontic treatment as some bacteria may be left behind. The purpose of this study was to evaluate the morphology of non-instrumented areas of the root canal wall using scanning-electron-microscopy (SEM) after completed instrumentation and various irrigation regiments. Materials and Methods Eighteen single-rooted extracted teeth were divided into the six groups. One tooth in each group represented a control sample. In all samples only one half of the canal was instrumented using ISO 40 hand files. Control samples were subjected to an irrigation protocols without instrumentation. Irrigants used were physiological saline, 3% sodium hypochlorite and 15% of ethylene-diamine-tetra-acetate. Irrigation protocol included using each of these irrigants alone, or a combination of NaOCl and EDTA, as well as their combination with final irrigation using NaOCl or chlorhexidine. Then after, roots were sectioned longitudinally and prepared for SEM. Results Saline irrigation left pulpal debris on uninstrumented areas of the canal wall. Irrigation with 3% NaOCl left behind canal wall with different forms of calcospherites. However, after EDTA irrigation dentin appeared as an undulating surface with open tubules without a smear layer. The combination of NaOCl and EDTA showed remnants of calcospherites and open slightly widened dentinal tubules. Final irrigation with NaOCl on the uninstrumented areas showed enlarged dentinal tubules along with dentinal erosion, while after final irrigation with CHX clean dentin and open dentinal tubules without smear layer were noted. Conclusion From the morphological point of view, the most favorable effect of irrigation on both uninstrumented and uninstrumented canal walls was achieved after irrigation with NaOCl and EDTA or NaOCl, EDTA and chlorhexidine as the final irrigant., Uvod Tokom endodontske terapije manje ili veće površine kanala korena zuba ostaju neinstrumentisane i mogu sadržavati bakterije, što može dovesti do neuspešnog ishoda. Cilj rada je bio da se skening-elekron-mikroskopskom (SEM) analizom proceni morfologija neistrumentisanih delova zidova kanala posle instrumentacije i primene različitih irigacionih protokola. Materijal i metode rada Osamnaest jednokorenih ekstrahovanih zuba je podeljeno u šest grupa. Jedan zub iz svake grupe je predstavljao kontrolni uzorak. Eksperimentalni uzorci su preparisani do instrumenta veličine ISO40. Kod svakog zuba je instrumentisana samo jedna polovina kanala. Kontrolni uzorci su podvrgnuti samo irigacionim protokolima bez preparacije. Osim fiziološkog rastvora, 3% natrijum-hipohlorita i 15% etilen-diamin-tetra-acetata, koji su primenjeni samostalno, eksperimentalni uzorci su irigirani i sa kombinacijom NaOCl i EDTA, kao i sa kombinacijom istih iriganasa, pri čemu je kao završni irigans korišćen ili NaOCl ili hlorheksidin. Korenovi su uzdužno presečeni i pripremljeni za SEM. Rezultati Posle irigacije sa fiziološkim rastvorom u neinstrumentisanim delovima kanala uočen je dentin pokriven debrisom, posle irigacije sa NaOCl dentin sa različitim oblicima kalcisferita. Posle irigacije sa EDTA uočena je talasasta površina dentinskog zida sa otvorenim tubulima bez razmaznog sloja. Irigacija kombinacijom NaOCl i EDTA je pokazala ostatke kalcisferita i otvorene, blago proširene dentinske tubule. Završno ispiranje sa NaOCl na neinstrumentisanim delovima dentina pokazuje proširene dentinske tubule i eroziju dentina, a završno ispiranje sa CHX čist dentin i otvorene dentinske tubule bez razmaznog sloja. Zaključak Sa morfološkog aspekta, najpovoljniji efekat irigacije (i kod instrumentisanih i kod neinstrumentisanih delova kanala) ustanovljen je posle irigacionih protokola sa: NaOCl i EDTA i NaOCl, EDTA i hlorheksidinom kao završnim irigansom.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Ultrastructural analysis of uninstrumented root canal areas following various irrigation regimens, Ultrastrukturna analiza neinstrumentisanih površina u kanalu korena posle različitih irigacionih protokola",
volume = "63",
number = "2",
pages = "57-65",
doi = "10.1515/sdj-2016-0006"
}

Barac, R., Popović, J., Živković, S., Mitić, A., Nikolić, M.,& Gašić, J.. (2016). Ultrastructural analysis of uninstrumented root canal areas following various irrigation regimens. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 63(2), 57-65.
https://doi.org/10.1515/sdj-2016-0006

Barac R, Popović J, Živković S, Mitić A, Nikolić M, Gašić J. Ultrastructural analysis of uninstrumented root canal areas following various irrigation regimens. in Stomatološki glasnik Srbije. 2016;63(2):57-65.
doi:10.1515/sdj-2016-0006 .

Barac, Radomir, Popović, Jelena, Živković, Slavoljub, Mitić, Aleksandar, Nikolić, Marija, Gašić, Jovanka, "Ultrastructural analysis of uninstrumented root canal areas following various irrigation regimens" in Stomatološki glasnik Srbije, 63, no. 2 (2016):57-65,
https://doi.org/10.1515/sdj-2016-0006 . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Radenković, Goran
AU  - Gašić, Jovanka
AU  - Mitić, Aleksandar
AU  - Nikolić, Marija
AU  - Barac, Radomir
AU  - Živković, Slavoljub
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2026
AB  - Introduction Separation (fracture) of endodontic instruments in the root canal during chemomechanical instrumentation is a complication that can compromise the final outcome of endodontic treatment. One of the most common factors that cause fatigue of endodontic instruments and consequent fracture is surface corrosion. The aim of this study was to investigate the ultrastructure of surface corrosion of endodontic instruments made of stainless steel and nickel-titanium after immersion in the most commonly used root canal irrigants. Material and Methods The study included 48 nickel-titanium and stainless steel endodontic hand files. All instruments were immersed in 5.25% sodium hypochlorite, 0.2% CHX and 17% EDTA. Surface corrosion was analyzed using a scanning electron microscope (SEM). Results Nickel-titanium instruments showed significantly higher susceptibility to corrosion after immersion in 5.25% sodium hypochlorite compared to stainless steel instruments (p lt 0,001). After immersion in 0.2% CHX corrosion damage was observed on both nickel-titanium and stainless steel instruments but the difference was not statistically significant (p=0.096). No corrosion was observed in both types of instruments after immersion in 17% EDTA. Conclusion The use of 5.25% NaOCl and 0.2% CHX as root canal irrigating solutions can cause serious corrosion changes on the surface of nickel-titanium and stainless steel endodontic instruments.
AB  - Uvod Fraktura endodontskih instrumenata u kanalu korena tokom hemomehaničke preparacije je značajna komplikacija koja može ugroziti konačan ishod endodontskog lečenja. Jedan od važnih faktora koji izazivaju zamor materijala endodontskog instrumenta je površinska korozija. Cilj ovog rada je bio da se ispita ultrastruktura površine endodontskih instrumenata od nerđajućeg čelika i nikl-titanijuma nakon potapanja u najčešće upotrebljavane rastvore za irigaciju kanala korena zuba. Materijal i metode rada U istraživanju je korišćeno 48 instrumenata od nikl-titanijuma i nerđajućeg čelika. Testiranje osetljivosti na koroziju je izvršeno potapanjem u rastvore NaOCl od 5,25%, CHX od 0,2% i EDTA od 17%. Analiza korozivnih oštećenja je urađena pomoću skening elektronskog mikroskopa (SEM) na različitim uveličanjima. Rezultati Instrumenti od nikl-titanijuma su pokazali statistički značajno veću osetljivost na koroziju nakon potapanja u 5,25% NaOCl u poređenju sa instrumentima od nerđajućeg čelika (p lt 0,001). Nakon potapanja u 0,2% CHX uočeno je korozivno oštećenje instrumenata od nikl-titanijuma i nerđajućeg čelika, ali razlika nije bila statistički značajna. Korozija nakon potapanja u 17% EDTA nije uočena ni kod jedne vrste endodontskih instrumenata. Zaključak Primena 5,25% NaOCl i 0,2% CHX kao rastvora za irigaciju kanala korena može izazvati ozbiljne korozivne promene na površini instrumenata od nikl-titanijuma i nerđajućeg čelika.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Ultrastructural analysis of the surface of endodontic instruments after immersion in irrigating solutions
T1  - Ultrastrukturna analiza površine endodontskih instrumenata nakon potapanja u rastvore za irigaciju
VL  - 62
IS  - 4
SP  - 157
EP  - 165
DO  - 10.1515/sdj-2015-0016
ER  - 

@article{
author = "Popović, Jelena and Radenković, Goran and Gašić, Jovanka and Mitić, Aleksandar and Nikolić, Marija and Barac, Radomir and Živković, Slavoljub",
year = "2015",
abstract = "Introduction Separation (fracture) of endodontic instruments in the root canal during chemomechanical instrumentation is a complication that can compromise the final outcome of endodontic treatment. One of the most common factors that cause fatigue of endodontic instruments and consequent fracture is surface corrosion. The aim of this study was to investigate the ultrastructure of surface corrosion of endodontic instruments made of stainless steel and nickel-titanium after immersion in the most commonly used root canal irrigants. Material and Methods The study included 48 nickel-titanium and stainless steel endodontic hand files. All instruments were immersed in 5.25% sodium hypochlorite, 0.2% CHX and 17% EDTA. Surface corrosion was analyzed using a scanning electron microscope (SEM). Results Nickel-titanium instruments showed significantly higher susceptibility to corrosion after immersion in 5.25% sodium hypochlorite compared to stainless steel instruments (p lt 0,001). After immersion in 0.2% CHX corrosion damage was observed on both nickel-titanium and stainless steel instruments but the difference was not statistically significant (p=0.096). No corrosion was observed in both types of instruments after immersion in 17% EDTA. Conclusion The use of 5.25% NaOCl and 0.2% CHX as root canal irrigating solutions can cause serious corrosion changes on the surface of nickel-titanium and stainless steel endodontic instruments., Uvod Fraktura endodontskih instrumenata u kanalu korena tokom hemomehaničke preparacije je značajna komplikacija koja može ugroziti konačan ishod endodontskog lečenja. Jedan od važnih faktora koji izazivaju zamor materijala endodontskog instrumenta je površinska korozija. Cilj ovog rada je bio da se ispita ultrastruktura površine endodontskih instrumenata od nerđajućeg čelika i nikl-titanijuma nakon potapanja u najčešće upotrebljavane rastvore za irigaciju kanala korena zuba. Materijal i metode rada U istraživanju je korišćeno 48 instrumenata od nikl-titanijuma i nerđajućeg čelika. Testiranje osetljivosti na koroziju je izvršeno potapanjem u rastvore NaOCl od 5,25%, CHX od 0,2% i EDTA od 17%. Analiza korozivnih oštećenja je urađena pomoću skening elektronskog mikroskopa (SEM) na različitim uveličanjima. Rezultati Instrumenti od nikl-titanijuma su pokazali statistički značajno veću osetljivost na koroziju nakon potapanja u 5,25% NaOCl u poređenju sa instrumentima od nerđajućeg čelika (p lt 0,001). Nakon potapanja u 0,2% CHX uočeno je korozivno oštećenje instrumenata od nikl-titanijuma i nerđajućeg čelika, ali razlika nije bila statistički značajna. Korozija nakon potapanja u 17% EDTA nije uočena ni kod jedne vrste endodontskih instrumenata. Zaključak Primena 5,25% NaOCl i 0,2% CHX kao rastvora za irigaciju kanala korena može izazvati ozbiljne korozivne promene na površini instrumenata od nikl-titanijuma i nerđajućeg čelika.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Ultrastructural analysis of the surface of endodontic instruments after immersion in irrigating solutions, Ultrastrukturna analiza površine endodontskih instrumenata nakon potapanja u rastvore za irigaciju",
volume = "62",
number = "4",
pages = "157-165",
doi = "10.1515/sdj-2015-0016"
}

Popović, J., Radenković, G., Gašić, J., Mitić, A., Nikolić, M., Barac, R.,& Živković, S.. (2015). Ultrastructural analysis of the surface of endodontic instruments after immersion in irrigating solutions. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 62(4), 157-165.
https://doi.org/10.1515/sdj-2015-0016

Popović J, Radenković G, Gašić J, Mitić A, Nikolić M, Barac R, Živković S. Ultrastructural analysis of the surface of endodontic instruments after immersion in irrigating solutions. in Stomatološki glasnik Srbije. 2015;62(4):157-165.
doi:10.1515/sdj-2015-0016 .

Popović, Jelena, Radenković, Goran, Gašić, Jovanka, Mitić, Aleksandar, Nikolić, Marija, Barac, Radomir, Živković, Slavoljub, "Ultrastructural analysis of the surface of endodontic instruments after immersion in irrigating solutions" in Stomatološki glasnik Srbije, 62, no. 4 (2015):157-165,
https://doi.org/10.1515/sdj-2015-0016 . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Gašić, Jovanka
AU  - Živković, Slavoljub
AU  - Kesić, Ljiljana
AU  - Mitić, Aleksandar
AU  - Nikolić, Marija
AU  - Milašin, Jelena
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2007
AB  - Objective: The aim of this study was to investigate the presence of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) in the tissue of chronic periapical lesions, and to compare the results in relation to the symptoms of patients and the size of the lesion. Methods: Periapical lesions analyzed in the study were collected from the roots of the teeth indicated for extraction. Samples were divided according to the symptoms into groups of symptomatic and asymptomatic, and according the size into groups of small and large lesions. Polymerase chain reaction was used to detect HCMV and EBV. The amplification was performed in a DNA Thermal Cycler (Hybaid). Results: Symptomatic lesions were 7.68 times more likely to be infected with HCMV than asymptomatic lesions (p  lt  0.001). Large symptomatic lesions were 73.50 times more likely to harbor HCMV than small symptomatic lesions (p  lt  0.001). Large symptomatic lesions were 7.64 times more likely to be infected with EBV than small symptomatic lesions (p = 0.05). Large symptomatic lesions were 5.38 times more likely to harbor dual HCMV/EBV infection than small symptomatic lesions (p = 0.115). Conclusion: Detection of HCMV and EBV in the samples of periapical lesions suggests an important role of herpesviruses in periapical tissue destruction.
PB  - Karger, Basel
T2  - Intervirology
T1  - Prevalence of Human Cytomegalovirus and Epstein-Barr Virus in Chronic Periapical Lesions
VL  - 58
IS  - 5
SP  - 271
EP  - 277
DO  - 10.1159/000441208
ER  - 

@article{
author = "Popović, Jelena and Gašić, Jovanka and Živković, Slavoljub and Kesić, Ljiljana and Mitić, Aleksandar and Nikolić, Marija and Milašin, Jelena",
year = "2015",
abstract = "Objective: The aim of this study was to investigate the presence of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) in the tissue of chronic periapical lesions, and to compare the results in relation to the symptoms of patients and the size of the lesion. Methods: Periapical lesions analyzed in the study were collected from the roots of the teeth indicated for extraction. Samples were divided according to the symptoms into groups of symptomatic and asymptomatic, and according the size into groups of small and large lesions. Polymerase chain reaction was used to detect HCMV and EBV. The amplification was performed in a DNA Thermal Cycler (Hybaid). Results: Symptomatic lesions were 7.68 times more likely to be infected with HCMV than asymptomatic lesions (p  lt  0.001). Large symptomatic lesions were 73.50 times more likely to harbor HCMV than small symptomatic lesions (p  lt  0.001). Large symptomatic lesions were 7.64 times more likely to be infected with EBV than small symptomatic lesions (p = 0.05). Large symptomatic lesions were 5.38 times more likely to harbor dual HCMV/EBV infection than small symptomatic lesions (p = 0.115). Conclusion: Detection of HCMV and EBV in the samples of periapical lesions suggests an important role of herpesviruses in periapical tissue destruction.",
publisher = "Karger, Basel",
journal = "Intervirology",
title = "Prevalence of Human Cytomegalovirus and Epstein-Barr Virus in Chronic Periapical Lesions",
volume = "58",
number = "5",
pages = "271-277",
doi = "10.1159/000441208"
}

Popović, J., Gašić, J., Živković, S., Kesić, L., Mitić, A., Nikolić, M.,& Milašin, J.. (2015). Prevalence of Human Cytomegalovirus and Epstein-Barr Virus in Chronic Periapical Lesions. in Intervirology
Karger, Basel., 58(5), 271-277.
https://doi.org/10.1159/000441208

Popović J, Gašić J, Živković S, Kesić L, Mitić A, Nikolić M, Milašin J. Prevalence of Human Cytomegalovirus and Epstein-Barr Virus in Chronic Periapical Lesions. in Intervirology. 2015;58(5):271-277.
doi:10.1159/000441208 .

Popović, Jelena, Gašić, Jovanka, Živković, Slavoljub, Kesić, Ljiljana, Mitić, Aleksandar, Nikolić, Marija, Milašin, Jelena, "Prevalence of Human Cytomegalovirus and Epstein-Barr Virus in Chronic Periapical Lesions" in Intervirology, 58, no. 5 (2015):271-277,
https://doi.org/10.1159/000441208 . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Gašić, Jovanka
AU  - Živković, Slavoljub
AU  - Mitić, Aleksandar
AU  - Nikolić, Marija
AU  - Barac, Radomir
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1864
AB  - Introduction Although many studies have reported various advantages of nickel-titanium endodontic instruments, the use of stainless steel endodontic files still presents a standard in everyday practice. The aim of this study was to evaluate ultrastructure of the surface of stainless steel endodontic instruments before and after their use. Material and Methods Fourteen sets of non-used stainless steel endodontic reamers and files were included in the study. Each set of instruments was used one, three or six times in simulated clinical conditions on extracted maxillary premolars. After their use the instruments were subjected to cleaning and sterilization procedures. Ultrastructure of the surface of instruments was observed under scanning electron microscope (SEM). Surface analysis was performed based on the number of uses and size of instruments. The following characteristics were analyzed: defects of instrument flutes, defects of cutting edges and instrument deformations. Results SEM analysis showed defects on the surface of non-used instruments as a result of the manufacturing process. Surface defects, defects of cutting edges as well as flutes deformations were observed after a single use and were more often noticed in small sized instruments. After three uses, flutes deformations were observed in all small sized instruments and some of the medium size. After six uses all groups of instruments showed defects of cutting edges. Conclusion Non-used stainless steel endodontic instruments showed surface defects created by the manufacturing process. Defects of cutting edges and flutes can be observed after a single use. To minimize complications and mistakes during clinical preparation it is recommended to limit the number of instrument uses.
AB  - Uvod Iako brojne studije ističu prednost korišćenja instrumenata od nikl-titanijuma zbog njihovih prednosti, primena endodontskih instrumenata od nerđajućeg čelika i dalje je standard u svakodnevnoj praksi. Cilj ovog rada je bio da se provere ultrastrukturne odlike površine radnog dela endodontskih instrumenata od nerđajućeg čelika pre i posle njihove upotrebe. Materijal i metode rada U istraživanju je korišćeno 14 setova novih ručnih proširivača i turpija od nerđajućeg čelika. Instrumenti svakog seta su korišćeni jednom, tri i šest puta za preparaciju kanala u simuliranim kliničkim uslovima na ekstrahovanim gornjim premolarima. Nakon svake upotrebe instrumenti su očišćeni i sterilizovani. Površina instrumenata je posmatrana i analizirana na skening-elektronskom mikroskopu (SEM). Analiza ultrastrukture instrumenata vršena je u pogledu broja upotreba i veličine. Kada su u pitanju ultrastrukturne promene na instrumentima, analizirani su oštećenja površine i sečiva i deformacije instrumenata. Rezultati SEM analiza je pokazala da i kod novih instrumenata postoje oštećenja koja su verovatno posledica proizvodnog procesa. Nakon prve upotrebe javljala su se oštećenja površine, sečivnih ivica, kao i deformacije navoja, i to češće kod manjih veličina. Nakon tri upotrebe deformiteti navoja su se javili kod svih instrumenata malih veličina i kod pojedinih instrumenata srednje velicine. Nakon šeste upotrebe dominirala su oštećenja u vidu tupljenja i zalamanja sečivnih ivica, koja su bila jednako zastupljena u svim grupama instrumenata. Zaključak Novi endodontski instrumenti od nerđajućegčelika imaju na svojoj površini oštećenja koja potiču od procesa proizvodnje, a deformacije instrumenata i oštećenja seciva se javljaju već posle prve kliničke upotrebe. Da bi se sprečile komplikacije i greške u kliničkom radu, preporuka je da broj upotreba endodontskih instrumenata bude ograničen.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Ultrastructural analysis of the surface of stainless steel endodontic instruments before and after use
T1  - Ultrastrukturna ispitivanja površine endodontskih instrumenata od nerđajućeg čelika
VL  - 61
IS  - 4
SP  - 175
EP  - 182
DO  - 10.2298/sgs1404175p
ER  - 

@article{
author = "Popović, Jelena and Gašić, Jovanka and Živković, Slavoljub and Mitić, Aleksandar and Nikolić, Marija and Barac, Radomir",
year = "2014",
abstract = "Introduction Although many studies have reported various advantages of nickel-titanium endodontic instruments, the use of stainless steel endodontic files still presents a standard in everyday practice. The aim of this study was to evaluate ultrastructure of the surface of stainless steel endodontic instruments before and after their use. Material and Methods Fourteen sets of non-used stainless steel endodontic reamers and files were included in the study. Each set of instruments was used one, three or six times in simulated clinical conditions on extracted maxillary premolars. After their use the instruments were subjected to cleaning and sterilization procedures. Ultrastructure of the surface of instruments was observed under scanning electron microscope (SEM). Surface analysis was performed based on the number of uses and size of instruments. The following characteristics were analyzed: defects of instrument flutes, defects of cutting edges and instrument deformations. Results SEM analysis showed defects on the surface of non-used instruments as a result of the manufacturing process. Surface defects, defects of cutting edges as well as flutes deformations were observed after a single use and were more often noticed in small sized instruments. After three uses, flutes deformations were observed in all small sized instruments and some of the medium size. After six uses all groups of instruments showed defects of cutting edges. Conclusion Non-used stainless steel endodontic instruments showed surface defects created by the manufacturing process. Defects of cutting edges and flutes can be observed after a single use. To minimize complications and mistakes during clinical preparation it is recommended to limit the number of instrument uses., Uvod Iako brojne studije ističu prednost korišćenja instrumenata od nikl-titanijuma zbog njihovih prednosti, primena endodontskih instrumenata od nerđajućeg čelika i dalje je standard u svakodnevnoj praksi. Cilj ovog rada je bio da se provere ultrastrukturne odlike površine radnog dela endodontskih instrumenata od nerđajućeg čelika pre i posle njihove upotrebe. Materijal i metode rada U istraživanju je korišćeno 14 setova novih ručnih proširivača i turpija od nerđajućeg čelika. Instrumenti svakog seta su korišćeni jednom, tri i šest puta za preparaciju kanala u simuliranim kliničkim uslovima na ekstrahovanim gornjim premolarima. Nakon svake upotrebe instrumenti su očišćeni i sterilizovani. Površina instrumenata je posmatrana i analizirana na skening-elektronskom mikroskopu (SEM). Analiza ultrastrukture instrumenata vršena je u pogledu broja upotreba i veličine. Kada su u pitanju ultrastrukturne promene na instrumentima, analizirani su oštećenja površine i sečiva i deformacije instrumenata. Rezultati SEM analiza je pokazala da i kod novih instrumenata postoje oštećenja koja su verovatno posledica proizvodnog procesa. Nakon prve upotrebe javljala su se oštećenja površine, sečivnih ivica, kao i deformacije navoja, i to češće kod manjih veličina. Nakon tri upotrebe deformiteti navoja su se javili kod svih instrumenata malih veličina i kod pojedinih instrumenata srednje velicine. Nakon šeste upotrebe dominirala su oštećenja u vidu tupljenja i zalamanja sečivnih ivica, koja su bila jednako zastupljena u svim grupama instrumenata. Zaključak Novi endodontski instrumenti od nerđajućegčelika imaju na svojoj površini oštećenja koja potiču od procesa proizvodnje, a deformacije instrumenata i oštećenja seciva se javljaju već posle prve kliničke upotrebe. Da bi se sprečile komplikacije i greške u kliničkom radu, preporuka je da broj upotreba endodontskih instrumenata bude ograničen.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Ultrastructural analysis of the surface of stainless steel endodontic instruments before and after use, Ultrastrukturna ispitivanja površine endodontskih instrumenata od nerđajućeg čelika",
volume = "61",
number = "4",
pages = "175-182",
doi = "10.2298/sgs1404175p"
}

Popović, J., Gašić, J., Živković, S., Mitić, A., Nikolić, M.,& Barac, R.. (2014). Ultrastructural analysis of the surface of stainless steel endodontic instruments before and after use. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 61(4), 175-182.
https://doi.org/10.2298/sgs1404175p

Popović J, Gašić J, Živković S, Mitić A, Nikolić M, Barac R. Ultrastructural analysis of the surface of stainless steel endodontic instruments before and after use. in Stomatološki glasnik Srbije. 2014;61(4):175-182.
doi:10.2298/sgs1404175p .

Popović, Jelena, Gašić, Jovanka, Živković, Slavoljub, Mitić, Aleksandar, Nikolić, Marija, Barac, Radomir, "Ultrastructural analysis of the surface of stainless steel endodontic instruments before and after use" in Stomatološki glasnik Srbije, 61, no. 4 (2014):175-182,
https://doi.org/10.2298/sgs1404175p . .

TY  - JOUR
AU  - Mitić, Aleksandar
AU  - Mitić, Nadica
AU  - Milašin, Jelena
AU  - Živković, Slavoljub
AU  - Gašić, Jovanka
AU  - Mitić, Vladimir
AU  - Popović, Jelena
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1795
AB  - Introduction. Clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic. Objective. The aim of the paper was to assess the presence of genomes of the most common microorganisms (Porphyromonas gingivalis, Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella intermedia, Treponema denticola and Enterococcus faecalis) in infected tooth root canals before and after rinsing with solution of doxycycline, citric acid and detergent Tween-80 (MTAD) in patients with clinically diagnosed primary apex periodontitis. Methods. The content of primarily infected canals before and after using the MTAD solution was used as a biological material in which the presence of microorganisms DNA was proved. For the detection of bacterial genome the multiplex PCR technique was applied. Results. The percentage of positive samples before canal treatment was 100%. In infected root canals E. faecalis was most dominant (37%). In a relatively high percentage we detected P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) and P. gingivalis (10%). After rinsing the canal system using MTAD solution, there was a statistically significant decrease in E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) and P. gingivalis (0%). The presence of other bacteria was also diminished but not statistically significantly. Conclusion. With the application of multiplex PCR technique which provided a simultaneous amplification of various genomic sequences, using several pairs of primers, the most dominant in infected root canals were E. faecalis. P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis and P. gingivalis. After mechanic treatment and irrigation of root canals with MTAD solution, P. intermedia, P. gingivalis and T. forsythensis were not found. The presence of E. faecalis, A. actinomycetemcomitans and T. denticola was diminished, however, not statistically significantly.
AB  - Uvod. Klinički prihvatljiv intrakanalni antiseptik mora imati organolitičko-mineralolitičko dejstvo i antibakterijsku efikasnost i ne sme biti toksičan. Cilj rada. Cilj rada je bio da se ispita postojanje genoma najčešćih mikroorganizama (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythensis, Prevotella intermedia, Treponema denticola i Enterococcus faecalis) u inficiranim kanalima korena zuba pre i posle irigacije rastvorom doksiciklina, limunske kiseline i deterdženta Tween­80 (MTAD) kod osoba s klinički dijagnostikovanim primarnim apeksnim parodontitisom. Metode rada. Kao biološki materijal u kojem je dokazivano prisustvo DNK mikroorganizama korišćen je sadržaj iz primarno inficiranih kanala pre i posle irigacije rastvorom MTAD. Za otkrivanje bakterijskog genoma primenjena je multipleksna tehnika PCR. Rezultati. Procenat pozitivnih uzoraka pre obrade kanala bio je 100%. U inficiranim kanalima korena zuba najčešći je bio E. faecalis (37%). U relativno visokom procentu otkriveni su i: P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) i P. gingivalis (10%). Posle irigacije kanalnog sistema rastvorom MTAD utvrđeno je statistički značajno smanjenje učestalosti E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) i P. gingivalis (0%). Učestalost ostalih bakterija takođe se smanjila, ali ne statistički značajno. Zaključak. Primenom multipleksne PCR tehnike, koja omogućava istovremenu amplifikaciju genskih sekvenci uz korišćenje dva para prajmera, u inficiranim kanalima korena zuba najčešće je utvrđen E. faecalis. U relativno visokom procentu otkrivene su P. intermedia, A. actinomycetem­comitans, T. denticola, T. forsythensis i P. gingivalis. Posle hemomehaničke obrade i irigacije kanala rastvorom MTAD nisu zabeležene P. intermedia, P. gingivalis i T. forsythensis, dok je učestalost E. faecalis, A. actinomycetemcomitans i T. denticola smanjena, ali ne statistički značajno.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique
T1  - Analiza antimikrobnog dejstva rastvora MTAD u inficiranom kanalnom sistemu korena zuba tehnikom PCR
VL  - 141
IS  - 3-4
SP  - 155
EP  - 162
DO  - 10.2298/SARH1304155M
ER  - 

@article{
author = "Mitić, Aleksandar and Mitić, Nadica and Milašin, Jelena and Živković, Slavoljub and Gašić, Jovanka and Mitić, Vladimir and Popović, Jelena",
year = "2013",
abstract = "Introduction. Clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic. Objective. The aim of the paper was to assess the presence of genomes of the most common microorganisms (Porphyromonas gingivalis, Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella intermedia, Treponema denticola and Enterococcus faecalis) in infected tooth root canals before and after rinsing with solution of doxycycline, citric acid and detergent Tween-80 (MTAD) in patients with clinically diagnosed primary apex periodontitis. Methods. The content of primarily infected canals before and after using the MTAD solution was used as a biological material in which the presence of microorganisms DNA was proved. For the detection of bacterial genome the multiplex PCR technique was applied. Results. The percentage of positive samples before canal treatment was 100%. In infected root canals E. faecalis was most dominant (37%). In a relatively high percentage we detected P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) and P. gingivalis (10%). After rinsing the canal system using MTAD solution, there was a statistically significant decrease in E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) and P. gingivalis (0%). The presence of other bacteria was also diminished but not statistically significantly. Conclusion. With the application of multiplex PCR technique which provided a simultaneous amplification of various genomic sequences, using several pairs of primers, the most dominant in infected root canals were E. faecalis. P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis and P. gingivalis. After mechanic treatment and irrigation of root canals with MTAD solution, P. intermedia, P. gingivalis and T. forsythensis were not found. The presence of E. faecalis, A. actinomycetemcomitans and T. denticola was diminished, however, not statistically significantly., Uvod. Klinički prihvatljiv intrakanalni antiseptik mora imati organolitičko-mineralolitičko dejstvo i antibakterijsku efikasnost i ne sme biti toksičan. Cilj rada. Cilj rada je bio da se ispita postojanje genoma najčešćih mikroorganizama (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythensis, Prevotella intermedia, Treponema denticola i Enterococcus faecalis) u inficiranim kanalima korena zuba pre i posle irigacije rastvorom doksiciklina, limunske kiseline i deterdženta Tween­80 (MTAD) kod osoba s klinički dijagnostikovanim primarnim apeksnim parodontitisom. Metode rada. Kao biološki materijal u kojem je dokazivano prisustvo DNK mikroorganizama korišćen je sadržaj iz primarno inficiranih kanala pre i posle irigacije rastvorom MTAD. Za otkrivanje bakterijskog genoma primenjena je multipleksna tehnika PCR. Rezultati. Procenat pozitivnih uzoraka pre obrade kanala bio je 100%. U inficiranim kanalima korena zuba najčešći je bio E. faecalis (37%). U relativno visokom procentu otkriveni su i: P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) i P. gingivalis (10%). Posle irigacije kanalnog sistema rastvorom MTAD utvrđeno je statistički značajno smanjenje učestalosti E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) i P. gingivalis (0%). Učestalost ostalih bakterija takođe se smanjila, ali ne statistički značajno. Zaključak. Primenom multipleksne PCR tehnike, koja omogućava istovremenu amplifikaciju genskih sekvenci uz korišćenje dva para prajmera, u inficiranim kanalima korena zuba najčešće je utvrđen E. faecalis. U relativno visokom procentu otkrivene su P. intermedia, A. actinomycetem­comitans, T. denticola, T. forsythensis i P. gingivalis. Posle hemomehaničke obrade i irigacije kanala rastvorom MTAD nisu zabeležene P. intermedia, P. gingivalis i T. forsythensis, dok je učestalost E. faecalis, A. actinomycetemcomitans i T. denticola smanjena, ali ne statistički značajno.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique, Analiza antimikrobnog dejstva rastvora MTAD u inficiranom kanalnom sistemu korena zuba tehnikom PCR",
volume = "141",
number = "3-4",
pages = "155-162",
doi = "10.2298/SARH1304155M"
}

Mitić, A., Mitić, N., Milašin, J., Živković, S., Gašić, J., Mitić, V.,& Popović, J.. (2013). Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 141(3-4), 155-162.
https://doi.org/10.2298/SARH1304155M

Mitić A, Mitić N, Milašin J, Živković S, Gašić J, Mitić V, Popović J. Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique. in Srpski arhiv za celokupno lekarstvo. 2013;141(3-4):155-162.
doi:10.2298/SARH1304155M .

Mitić, Aleksandar, Mitić, Nadica, Milašin, Jelena, Živković, Slavoljub, Gašić, Jovanka, Mitić, Vladimir, Popović, Jelena, "Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique" in Srpski arhiv za celokupno lekarstvo, 141, no. 3-4 (2013):155-162,
https://doi.org/10.2298/SARH1304155M . .

TY  - JOUR
AU  - Gašić, Jovanka
AU  - Popović, Jelena
AU  - Živković, Slavoljub
AU  - Petrović, Aleksandar
AU  - Barac, Radomir
AU  - Nikolić, Marija
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1764
AB  - To determine whether sodium hypochlorite (NaOCl) with 0.2% chlorhexidine gluconate (CHX) leads to colour change and precipitate formation, and to ultrastructurally analyse the dentine surface after simultaneous irrigation with 0.5% NaOCl and 0.2% CHX. Four tubes in which 5ml of different NaOCl concentrations and 5ml 0,2% CHX were placed, were observed every 15 minutes in the first two hours and after 7 days. Mixture solutions were centrifuged at 800 rpm/4 min. A precipitate is observed under light-microscopy. Thirty-five single-rooted teeth were instrumented using crown-down technique with irrigation: Positive control: distilled water, Negative control: 0,5% NaOCl+15% EDTA, Experimental group: identical to the negative control, then canals were treated with 0.5% NaOCl+0.2% CHX. The longitudinal root sections were observed under scanning-electron-microscopy. The amount of debris was assessed with 5/score-system, and the results were analysed using the Mann-Whitney U test (p  lt  0.001). Change in colour was noticed immediately after the merger and it did not change with time. Mixture solutions showed considerable turbidity, but precipitate was observed only after centrifugation. Statistical analysis showed a significant difference in the dentinal wall purity when comparing the cervical and middle root canal thirds between the experimental and negative control group. The difference was also observed between the cervical and middle thirds compared to the apical third of the root canal within these groups. Interaction between NaOCl and CHX, as well as the creation of precipitates, depends not only on the concentration of NaOCl, but also on the concentration of CHX. Microsc. Res. Tech. 75:10991103, 2012.
PB  - Wiley, Hoboken
T2  - Microscopy Research & Technique
T1  - Ultrastructural analysis of the root canal walls after simultaneous irrigation of different sodium hypochlorite concentration and 0.2% chlorhexidine gluconate
VL  - 75
IS  - 8
SP  - 1099
EP  - 1103
DO  - 10.1002/jemt.22036
ER  - 

@article{
author = "Gašić, Jovanka and Popović, Jelena and Živković, Slavoljub and Petrović, Aleksandar and Barac, Radomir and Nikolić, Marija",
year = "2012",
abstract = "To determine whether sodium hypochlorite (NaOCl) with 0.2% chlorhexidine gluconate (CHX) leads to colour change and precipitate formation, and to ultrastructurally analyse the dentine surface after simultaneous irrigation with 0.5% NaOCl and 0.2% CHX. Four tubes in which 5ml of different NaOCl concentrations and 5ml 0,2% CHX were placed, were observed every 15 minutes in the first two hours and after 7 days. Mixture solutions were centrifuged at 800 rpm/4 min. A precipitate is observed under light-microscopy. Thirty-five single-rooted teeth were instrumented using crown-down technique with irrigation: Positive control: distilled water, Negative control: 0,5% NaOCl+15% EDTA, Experimental group: identical to the negative control, then canals were treated with 0.5% NaOCl+0.2% CHX. The longitudinal root sections were observed under scanning-electron-microscopy. The amount of debris was assessed with 5/score-system, and the results were analysed using the Mann-Whitney U test (p  lt  0.001). Change in colour was noticed immediately after the merger and it did not change with time. Mixture solutions showed considerable turbidity, but precipitate was observed only after centrifugation. Statistical analysis showed a significant difference in the dentinal wall purity when comparing the cervical and middle root canal thirds between the experimental and negative control group. The difference was also observed between the cervical and middle thirds compared to the apical third of the root canal within these groups. Interaction between NaOCl and CHX, as well as the creation of precipitates, depends not only on the concentration of NaOCl, but also on the concentration of CHX. Microsc. Res. Tech. 75:10991103, 2012.",
publisher = "Wiley, Hoboken",
journal = "Microscopy Research & Technique",
title = "Ultrastructural analysis of the root canal walls after simultaneous irrigation of different sodium hypochlorite concentration and 0.2% chlorhexidine gluconate",
volume = "75",
number = "8",
pages = "1099-1103",
doi = "10.1002/jemt.22036"
}

Gašić, J., Popović, J., Živković, S., Petrović, A., Barac, R.,& Nikolić, M.. (2012). Ultrastructural analysis of the root canal walls after simultaneous irrigation of different sodium hypochlorite concentration and 0.2% chlorhexidine gluconate. in Microscopy Research & Technique
Wiley, Hoboken., 75(8), 1099-1103.
https://doi.org/10.1002/jemt.22036

Gašić J, Popović J, Živković S, Petrović A, Barac R, Nikolić M. Ultrastructural analysis of the root canal walls after simultaneous irrigation of different sodium hypochlorite concentration and 0.2% chlorhexidine gluconate. in Microscopy Research & Technique. 2012;75(8):1099-1103.
doi:10.1002/jemt.22036 .

Gašić, Jovanka, Popović, Jelena, Živković, Slavoljub, Petrović, Aleksandar, Barac, Radomir, Nikolić, Marija, "Ultrastructural analysis of the root canal walls after simultaneous irrigation of different sodium hypochlorite concentration and 0.2% chlorhexidine gluconate" in Microscopy Research & Technique, 75, no. 8 (2012):1099-1103,
https://doi.org/10.1002/jemt.22036 . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Gašić, Jovanka
AU  - Živković, Slavoljub
AU  - Petrović, A.
AU  - Radičević, Goran
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1529
AB  - Aim To examine the presence of biological debris and the level of contamination on reusable endodontic instruments those were subjected to different cleaning methods prior to sterilization. Methodology One hundred and eighty endodontic instruments from eighteen dental practices were analysed. These practices used different decontamination protocols for reusable instruments. The presence of organic debris was detected by the use of Van Gieson's stain. Forty-eight new stainless steel hand instruments were used as controls. The samples were examined by light microscopy. Results Residual biological debriswasobserved in 96% of the samples. The mean value of maximum biological contamination was 34% in the group in which the instruments were brushed manually and immersed in alcohol, 25% in the group in which commercially available disinfectants were used and 5% in the group in which the instruments were cleaned ultrasonically. There was a statistically significant difference in the mean values with respect to the cleaning protocol applied (P  lt  0.001). Conclusions The methods used to clean endodontic instruments appear to be generally ineffective for the removal of biological debris. The best method was the one that included mechanical, chemical and ultrasonic cleaning of instruments.
PB  - Wiley-Blackwell Publishing, Inc, Malden
T2  - International Endodontic Journal
T1  - Evaluation of biological debris on endodontic instruments after cleaning and sterilization procedures
VL  - 43
IS  - 4
SP  - 336
EP  - 341
DO  - 10.1111/j.1365-2591.2010.01686.x
ER  - 

@article{
author = "Popović, Jelena and Gašić, Jovanka and Živković, Slavoljub and Petrović, A. and Radičević, Goran",
year = "2010",
abstract = "Aim To examine the presence of biological debris and the level of contamination on reusable endodontic instruments those were subjected to different cleaning methods prior to sterilization. Methodology One hundred and eighty endodontic instruments from eighteen dental practices were analysed. These practices used different decontamination protocols for reusable instruments. The presence of organic debris was detected by the use of Van Gieson's stain. Forty-eight new stainless steel hand instruments were used as controls. The samples were examined by light microscopy. Results Residual biological debriswasobserved in 96% of the samples. The mean value of maximum biological contamination was 34% in the group in which the instruments were brushed manually and immersed in alcohol, 25% in the group in which commercially available disinfectants were used and 5% in the group in which the instruments were cleaned ultrasonically. There was a statistically significant difference in the mean values with respect to the cleaning protocol applied (P  lt  0.001). Conclusions The methods used to clean endodontic instruments appear to be generally ineffective for the removal of biological debris. The best method was the one that included mechanical, chemical and ultrasonic cleaning of instruments.",
publisher = "Wiley-Blackwell Publishing, Inc, Malden",
journal = "International Endodontic Journal",
title = "Evaluation of biological debris on endodontic instruments after cleaning and sterilization procedures",
volume = "43",
number = "4",
pages = "336-341",
doi = "10.1111/j.1365-2591.2010.01686.x"
}

Popović, J., Gašić, J., Živković, S., Petrović, A.,& Radičević, G.. (2010). Evaluation of biological debris on endodontic instruments after cleaning and sterilization procedures. in International Endodontic Journal
Wiley-Blackwell Publishing, Inc, Malden., 43(4), 336-341.
https://doi.org/10.1111/j.1365-2591.2010.01686.x

Popović J, Gašić J, Živković S, Petrović A, Radičević G. Evaluation of biological debris on endodontic instruments after cleaning and sterilization procedures. in International Endodontic Journal. 2010;43(4):336-341.
doi:10.1111/j.1365-2591.2010.01686.x .

Popović, Jelena, Gašić, Jovanka, Živković, Slavoljub, Petrović, A., Radičević, Goran, "Evaluation of biological debris on endodontic instruments after cleaning and sterilization procedures" in International Endodontic Journal, 43, no. 4 (2010):336-341,
https://doi.org/10.1111/j.1365-2591.2010.01686.x . .