TY  - JOUR
AU  - Toljić, Boško
AU  - Trbovich, Alexander M.
AU  - Matić-Petrović, Sanja
AU  - Kannosh, Ibrahim
AU  - Dragović, Gordana
AU  - Jevtović, Đorđe
AU  - de Luka, Silvio R.
AU  - Ristic-Đurović, Jasna L.
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2269
AB  - The importance of oral microflora composition in HIV-infected patients is well recognized. However, no studies so far have dealt with age-related changes in periodontal pathogens occurrence in HIV+ individuals. The aim of the present study was to assess and compare temporal changes of bacteria frequency in younger ( lt = 35 years) and older (>= 50 years) HIV-infected and non-infected individuals. Bacterial DNA was isolated from buccal swabs of 30 younger and 30 older subjects in both HIV+ and HIV-groups. By means of PCR the following microorganisms were detected: Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Peptostreptococcus micros, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and Treponema denticola. Oral and periodontal examinations were performed in all subjects. The prevalence of microorganisms was significantly higher in HIV+ patients compared to controls, and their distribution showed a notable shift. The decreasing incidence in HIV-subjects was: Pi>Pm>Pg>Aa>Ec>Tf>Td whilst in HIV+ it was: Pi>Pm>Ec>Pg>Tf>Aa>Td. Oral manifestations of HIV infection were more frequent in older compared to younger patients. All measured values of clinical periodontal parameters were significantly higher in older compared to younger HIV+ patients. Ageing in HIV+ subjects is accompanied with a substantial increase and rearrangements of periodontal microflora, potentially aggravating oral and systemic health.
PB  - Luigi Ponzio e figlio Editori
T2  - New Microbiologica
T1  - Ageing with HIV - a periodontal perspective
VL  - 41
IS  - 1
SP  - 61
EP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_smile_2269
ER  - 

@article{
author = "Toljić, Boško and Trbovich, Alexander M. and Matić-Petrović, Sanja and Kannosh, Ibrahim and Dragović, Gordana and Jevtović, Đorđe and de Luka, Silvio R. and Ristic-Đurović, Jasna L. and Milašin, Jelena",
year = "2018",
abstract = "The importance of oral microflora composition in HIV-infected patients is well recognized. However, no studies so far have dealt with age-related changes in periodontal pathogens occurrence in HIV+ individuals. The aim of the present study was to assess and compare temporal changes of bacteria frequency in younger ( lt = 35 years) and older (>= 50 years) HIV-infected and non-infected individuals. Bacterial DNA was isolated from buccal swabs of 30 younger and 30 older subjects in both HIV+ and HIV-groups. By means of PCR the following microorganisms were detected: Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Peptostreptococcus micros, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and Treponema denticola. Oral and periodontal examinations were performed in all subjects. The prevalence of microorganisms was significantly higher in HIV+ patients compared to controls, and their distribution showed a notable shift. The decreasing incidence in HIV-subjects was: Pi>Pm>Pg>Aa>Ec>Tf>Td whilst in HIV+ it was: Pi>Pm>Ec>Pg>Tf>Aa>Td. Oral manifestations of HIV infection were more frequent in older compared to younger patients. All measured values of clinical periodontal parameters were significantly higher in older compared to younger HIV+ patients. Ageing in HIV+ subjects is accompanied with a substantial increase and rearrangements of periodontal microflora, potentially aggravating oral and systemic health.",
publisher = "Luigi Ponzio e figlio Editori",
journal = "New Microbiologica",
title = "Ageing with HIV - a periodontal perspective",
volume = "41",
number = "1",
pages = "61-66",
url = "https://hdl.handle.net/21.15107/rcub_smile_2269"
}

Toljić, B., Trbovich, A. M., Matić-Petrović, S., Kannosh, I., Dragović, G., Jevtović, Đ., de Luka, S. R., Ristic-Đurović, J. L.,& Milašin, J.. (2018). Ageing with HIV - a periodontal perspective. in New Microbiologica
Luigi Ponzio e figlio Editori., 41(1), 61-66.
https://hdl.handle.net/21.15107/rcub_smile_2269

Toljić B, Trbovich AM, Matić-Petrović S, Kannosh I, Dragović G, Jevtović Đ, de Luka SR, Ristic-Đurović JL, Milašin J. Ageing with HIV - a periodontal perspective. in New Microbiologica. 2018;41(1):61-66.
https://hdl.handle.net/21.15107/rcub_smile_2269 .

Toljić, Boško, Trbovich, Alexander M., Matić-Petrović, Sanja, Kannosh, Ibrahim, Dragović, Gordana, Jevtović, Đorđe, de Luka, Silvio R., Ristic-Đurović, Jasna L., Milašin, Jelena, "Ageing with HIV - a periodontal perspective" in New Microbiologica, 41, no. 1 (2018):61-66,
https://hdl.handle.net/21.15107/rcub_smile_2269 .

TY  - JOUR
AU  - Kannosh, Ibrahim
AU  - Staletović, Danijela
AU  - Toljić, Boško
AU  - Radunović, Milena
AU  - Pucar, Ana
AU  - Matić-Petrović, Sanja
AU  - Grubiša, Ivana
AU  - Lazarević, Miloš
AU  - Brkić, Zlata
AU  - Knežević-Vukcević, Jelena
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2276
AB  - Introduction: There is a known connection between periodontitis and atherosclerosis and the presence of periopathogens in blood vessels. However, changes of the oral microflora related to the aging process and its possible effects on atherosclerosis, have yet to be analyzed. The aim of this study was to assess temporal changes in the frequency of periodontal bacteria in the subgingival plaque and in atherosclerotic blood vessels of patients with atherosclerosis. Methodology: The study included 100 patients with atherosclerosis and periodontitis, divided into two groups, below and over 60 years of age. Clinical examinations were performedand subgingival plaque specimens were collected as well as biopsy specimens from the following arteries: coronary (34), carotid (29), abdominal (10), femoral (10), mammary (13) and iliac (4). Subgingival and artery specimens were subjected to PCR detection of 5 major periodontal pathogens: Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Aggregatibacter actinomycetemcomitans (Aa), Tannerella forsythensis (Tf) and Treponema denticola (Td). Results: Tf was the most and Td the least frequent bacteria in both age groups and in both types of samples. The frequencies of bacteria in subgingival versus atherosclerotic samples were: Tf (76%: 53%), Pi (71%: 31%), Pg (60%: 38%), Aa (39%: 14%) and Td (21%: 6%). Only Aa and Pi showed a significant difference of prevalence between younger and older patients. The most colonized artery was a. coronaria, followed by a. carotis, a. abdominalis, a. mammaria, and a. femoralis. Conclusions: Patient's age and the distance of a given blood vessel from the oral cavity influenced microbiological findings in the atherotic plaque.
PB  - J Infection Developing Countries, Tramaniglio
T2  - Journal of Infection in Developing Countries
T1  - The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis
VL  - 12
IS  - 12
SP  - 1088
EP  - 1095
DO  - 10.3855/jidc.10980
ER  - 

@article{
author = "Kannosh, Ibrahim and Staletović, Danijela and Toljić, Boško and Radunović, Milena and Pucar, Ana and Matić-Petrović, Sanja and Grubiša, Ivana and Lazarević, Miloš and Brkić, Zlata and Knežević-Vukcević, Jelena and Milašin, Jelena",
year = "2018",
abstract = "Introduction: There is a known connection between periodontitis and atherosclerosis and the presence of periopathogens in blood vessels. However, changes of the oral microflora related to the aging process and its possible effects on atherosclerosis, have yet to be analyzed. The aim of this study was to assess temporal changes in the frequency of periodontal bacteria in the subgingival plaque and in atherosclerotic blood vessels of patients with atherosclerosis. Methodology: The study included 100 patients with atherosclerosis and periodontitis, divided into two groups, below and over 60 years of age. Clinical examinations were performedand subgingival plaque specimens were collected as well as biopsy specimens from the following arteries: coronary (34), carotid (29), abdominal (10), femoral (10), mammary (13) and iliac (4). Subgingival and artery specimens were subjected to PCR detection of 5 major periodontal pathogens: Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Aggregatibacter actinomycetemcomitans (Aa), Tannerella forsythensis (Tf) and Treponema denticola (Td). Results: Tf was the most and Td the least frequent bacteria in both age groups and in both types of samples. The frequencies of bacteria in subgingival versus atherosclerotic samples were: Tf (76%: 53%), Pi (71%: 31%), Pg (60%: 38%), Aa (39%: 14%) and Td (21%: 6%). Only Aa and Pi showed a significant difference of prevalence between younger and older patients. The most colonized artery was a. coronaria, followed by a. carotis, a. abdominalis, a. mammaria, and a. femoralis. Conclusions: Patient's age and the distance of a given blood vessel from the oral cavity influenced microbiological findings in the atherotic plaque.",
publisher = "J Infection Developing Countries, Tramaniglio",
journal = "Journal of Infection in Developing Countries",
title = "The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis",
volume = "12",
number = "12",
pages = "1088-1095",
doi = "10.3855/jidc.10980"
}

Kannosh, I., Staletović, D., Toljić, B., Radunović, M., Pucar, A., Matić-Petrović, S., Grubiša, I., Lazarević, M., Brkić, Z., Knežević-Vukcević, J.,& Milašin, J.. (2018). The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis. in Journal of Infection in Developing Countries
J Infection Developing Countries, Tramaniglio., 12(12), 1088-1095.
https://doi.org/10.3855/jidc.10980

Kannosh I, Staletović D, Toljić B, Radunović M, Pucar A, Matić-Petrović S, Grubiša I, Lazarević M, Brkić Z, Knežević-Vukcević J, Milašin J. The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis. in Journal of Infection in Developing Countries. 2018;12(12):1088-1095.
doi:10.3855/jidc.10980 .

Kannosh, Ibrahim, Staletović, Danijela, Toljić, Boško, Radunović, Milena, Pucar, Ana, Matić-Petrović, Sanja, Grubiša, Ivana, Lazarević, Miloš, Brkić, Zlata, Knežević-Vukcević, Jelena, Milašin, Jelena, "The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis" in Journal of Infection in Developing Countries, 12, no. 12 (2018):1088-1095,
https://doi.org/10.3855/jidc.10980 . .

TY  - JOUR
AU  - Petrović, M. S.
AU  - Kannosh, Ibrahim
AU  - Milašin, Jelena
AU  - Mihailović, D. S.
AU  - Obradović, Radmila
AU  - Bubanj, S. R.
AU  - Kesić, Ljiljana
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2286
AB  - ObjectiveA clinical prospective study was designed to evaluate microbiological, cytomorphometric and clinical efficacy of low-level laser therapy (LLLT) as an adjunct to periodontal therapy in the treatment of chronic periodontitis. MethodsSixty subjects were included and randomly assigned into 2 groups: SRP (scaling root planning) group (n=30) and LLLT+SRP group (n=30). Clinical parameters were measured before intervention, after the fifth treatment, and after a month. All subjects received oral hygiene instructions and full-mouth conservative periodontal treatment (removal of dental plaque followed by SRP). Afterwards, in group II, Kavo LLLT (980nm, 0.2W, 6J/cm(2)) was applied. Subgingival samples were collected at baseline and after the fifth treatment to quantify Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythensis and Treponema denticola by polymerase chain reaction (PCR). Gingival swabs were taken, and direct smears were prepared on slides for cytomorphometric analysis. ResultsEvaluation using clinical parameters showed better results in LLLT group. A statistically significant decrease in the prevalence of bacteria after treatment in LLLT group was observed for the following: T.forsythensis and T.denticola (P lt .001), P.gingivalis (P lt .01), A.actinomycetemcomitans and P.intermedia (P lt .05). The values of nuclear area, perimeter and Ferret's diameter were significantly lower in both studied groups after treatment, but statistical significance was higher in LLLT group (P lt .001) than in the SRP therapy group (P lt .05). ConclusionLow-level laser therapy as an adjunct to periodontal therapy demonstrates short-term additional bacteriological, cytological and clinical benefits.
PB  - Wiley, Hoboken
T2  - International Journal of Dental Hygiene
T1  - Clinical, microbiological and cytomorphometric evaluation of low-level laser therapy as an adjunct to periodontal therapy in patients with chronic periodontitis
VL  - 16
IS  - 2
SP  - E120
EP  - E127
DO  - 10.1111/idh.12328
ER  - 

@article{
author = "Petrović, M. S. and Kannosh, Ibrahim and Milašin, Jelena and Mihailović, D. S. and Obradović, Radmila and Bubanj, S. R. and Kesić, Ljiljana",
year = "2018",
abstract = "ObjectiveA clinical prospective study was designed to evaluate microbiological, cytomorphometric and clinical efficacy of low-level laser therapy (LLLT) as an adjunct to periodontal therapy in the treatment of chronic periodontitis. MethodsSixty subjects were included and randomly assigned into 2 groups: SRP (scaling root planning) group (n=30) and LLLT+SRP group (n=30). Clinical parameters were measured before intervention, after the fifth treatment, and after a month. All subjects received oral hygiene instructions and full-mouth conservative periodontal treatment (removal of dental plaque followed by SRP). Afterwards, in group II, Kavo LLLT (980nm, 0.2W, 6J/cm(2)) was applied. Subgingival samples were collected at baseline and after the fifth treatment to quantify Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythensis and Treponema denticola by polymerase chain reaction (PCR). Gingival swabs were taken, and direct smears were prepared on slides for cytomorphometric analysis. ResultsEvaluation using clinical parameters showed better results in LLLT group. A statistically significant decrease in the prevalence of bacteria after treatment in LLLT group was observed for the following: T.forsythensis and T.denticola (P lt .001), P.gingivalis (P lt .01), A.actinomycetemcomitans and P.intermedia (P lt .05). The values of nuclear area, perimeter and Ferret's diameter were significantly lower in both studied groups after treatment, but statistical significance was higher in LLLT group (P lt .001) than in the SRP therapy group (P lt .05). ConclusionLow-level laser therapy as an adjunct to periodontal therapy demonstrates short-term additional bacteriological, cytological and clinical benefits.",
publisher = "Wiley, Hoboken",
journal = "International Journal of Dental Hygiene",
title = "Clinical, microbiological and cytomorphometric evaluation of low-level laser therapy as an adjunct to periodontal therapy in patients with chronic periodontitis",
volume = "16",
number = "2",
pages = "E120-E127",
doi = "10.1111/idh.12328"
}

Petrović, M. S., Kannosh, I., Milašin, J., Mihailović, D. S., Obradović, R., Bubanj, S. R.,& Kesić, L.. (2018). Clinical, microbiological and cytomorphometric evaluation of low-level laser therapy as an adjunct to periodontal therapy in patients with chronic periodontitis. in International Journal of Dental Hygiene
Wiley, Hoboken., 16(2), E120-E127.
https://doi.org/10.1111/idh.12328

Petrović MS, Kannosh I, Milašin J, Mihailović DS, Obradović R, Bubanj SR, Kesić L. Clinical, microbiological and cytomorphometric evaluation of low-level laser therapy as an adjunct to periodontal therapy in patients with chronic periodontitis. in International Journal of Dental Hygiene. 2018;16(2):E120-E127.
doi:10.1111/idh.12328 .

Petrović, M. S., Kannosh, Ibrahim, Milašin, Jelena, Mihailović, D. S., Obradović, Radmila, Bubanj, S. R., Kesić, Ljiljana, "Clinical, microbiological and cytomorphometric evaluation of low-level laser therapy as an adjunct to periodontal therapy in patients with chronic periodontitis" in International Journal of Dental Hygiene, 16, no. 2 (2018):E120-E127,
https://doi.org/10.1111/idh.12328 . .

TY  - JOUR
AU  - Anđelković, Marko
AU  - Tihaček-Šojić, Ljiljana
AU  - Milić-Lemić, Aleksandra
AU  - Nikolić, Nadja
AU  - Kannosh, Ibrahim
AU  - Milašin, Jelena
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2194
AB  - Purpose: To determine if wearing complete dentures can cause changes in prevalence of some of the most common periodontal pathogens in elderly edentulous patients. The need for understanding the composition of oral microflora in edentulous patients has been recognized by some authors, but no studies have dealt with the changes that occur in periodontal pathogens' prevalence as a result of complete dentures. Materials and Methods: A total of 30 edentulous elderly (average age 71) patients participated in the study. Complete dentures were fabricated for each patient, and the residual alveolar ridges were swabbed before denture insertion. After a period of 6 months swabs were taken again. Identification of P. intermedia, A. actinomycetemcomitans, P. gingivalis, T. forsythia, T. denticola, and F. nucleatum was done by the polymerase chain reaction (PCR) method and primers specific for each microorganism. Results: A noticeable increase in the presence of periodontal pathogens was observed after 6 months of denture wearing; targeted bacteria were identified in 17 pre-insertion samples compared to 28 post-insertion samples. The McNemar test was used to compare the prevalence of periodontal pathogenic bacteria before and after dental treatment. p lt 0.05 indicated statistical significance. Three microorganisms showed a statistically significant difference between the first and second swabbing-A. actinomycetemcomitans (6.7% vs. 40.0%, p = 0.006), P. intermedia (30.0% vs. 73.3%, p = 0.004), and T. forsythia (6.7% vs. 30.0%, p = 0.004). There was also an increase in bacteria co-associations 6 months post-insertion of complete dentures. Conclusions: The results of the present study suggested that wearing complete dentures caused a considerable increase of periodontopathic bacteria prevalence in elderly patients. Better understanding of oral microflora and the impact dental treatment has on bacterial colonies is important in modern dentistry.
PB  - Wiley, Hoboken
T2  - Journal of Prosthodontics-Implant Esthetic & Reconstructive Dentistry
T1  - Does the Prevalence of Periodontal Pathogens Change in Elderly Edentulous Patients after Complete Denture Treatment?
VL  - 26
IS  - 5
SP  - 364
EP  - 369
DO  - 10.1111/jopr.12402
ER  - 

@article{
author = "Anđelković, Marko and Tihaček-Šojić, Ljiljana and Milić-Lemić, Aleksandra and Nikolić, Nadja and Kannosh, Ibrahim and Milašin, Jelena",
year = "2017",
abstract = "Purpose: To determine if wearing complete dentures can cause changes in prevalence of some of the most common periodontal pathogens in elderly edentulous patients. The need for understanding the composition of oral microflora in edentulous patients has been recognized by some authors, but no studies have dealt with the changes that occur in periodontal pathogens' prevalence as a result of complete dentures. Materials and Methods: A total of 30 edentulous elderly (average age 71) patients participated in the study. Complete dentures were fabricated for each patient, and the residual alveolar ridges were swabbed before denture insertion. After a period of 6 months swabs were taken again. Identification of P. intermedia, A. actinomycetemcomitans, P. gingivalis, T. forsythia, T. denticola, and F. nucleatum was done by the polymerase chain reaction (PCR) method and primers specific for each microorganism. Results: A noticeable increase in the presence of periodontal pathogens was observed after 6 months of denture wearing; targeted bacteria were identified in 17 pre-insertion samples compared to 28 post-insertion samples. The McNemar test was used to compare the prevalence of periodontal pathogenic bacteria before and after dental treatment. p lt 0.05 indicated statistical significance. Three microorganisms showed a statistically significant difference between the first and second swabbing-A. actinomycetemcomitans (6.7% vs. 40.0%, p = 0.006), P. intermedia (30.0% vs. 73.3%, p = 0.004), and T. forsythia (6.7% vs. 30.0%, p = 0.004). There was also an increase in bacteria co-associations 6 months post-insertion of complete dentures. Conclusions: The results of the present study suggested that wearing complete dentures caused a considerable increase of periodontopathic bacteria prevalence in elderly patients. Better understanding of oral microflora and the impact dental treatment has on bacterial colonies is important in modern dentistry.",
publisher = "Wiley, Hoboken",
journal = "Journal of Prosthodontics-Implant Esthetic & Reconstructive Dentistry",
title = "Does the Prevalence of Periodontal Pathogens Change in Elderly Edentulous Patients after Complete Denture Treatment?",
volume = "26",
number = "5",
pages = "364-369",
doi = "10.1111/jopr.12402"
}

Anđelković, M., Tihaček-Šojić, L., Milić-Lemić, A., Nikolić, N., Kannosh, I.,& Milašin, J.. (2017). Does the Prevalence of Periodontal Pathogens Change in Elderly Edentulous Patients after Complete Denture Treatment?. in Journal of Prosthodontics-Implant Esthetic & Reconstructive Dentistry
Wiley, Hoboken., 26(5), 364-369.
https://doi.org/10.1111/jopr.12402

Anđelković M, Tihaček-Šojić L, Milić-Lemić A, Nikolić N, Kannosh I, Milašin J. Does the Prevalence of Periodontal Pathogens Change in Elderly Edentulous Patients after Complete Denture Treatment?. in Journal of Prosthodontics-Implant Esthetic & Reconstructive Dentistry. 2017;26(5):364-369.
doi:10.1111/jopr.12402 .

Anđelković, Marko, Tihaček-Šojić, Ljiljana, Milić-Lemić, Aleksandra, Nikolić, Nadja, Kannosh, Ibrahim, Milašin, Jelena, "Does the Prevalence of Periodontal Pathogens Change in Elderly Edentulous Patients after Complete Denture Treatment?" in Journal of Prosthodontics-Implant Esthetic & Reconstructive Dentistry, 26, no. 5 (2017):364-369,
https://doi.org/10.1111/jopr.12402 . .