Detection of early laboratory fungal biomarkers and it's importance for outcome of invasive fungal infections in Serbia

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Detection of early laboratory fungal biomarkers and it's importance for outcome of invasive fungal infections in Serbia (en)
Значај доказивања раних лабораторијских биомаркера за исход инвазивних гљивичних инфекција код нас (sr)
Značaj dokazivanja ranih laboratorijskih biomarkera za ishod invazivnih gljivičnih infekcija kod nas (sr_RS)
Authors

Publications

Development and validation of mathematical models for testing antifungal activity of different essential oils against Candida species

Perić, Mirjana; Rajković, Katarina; Milić-Lemić, Aleksandra; Živković, Rade; Arsić-Arsenijević, Valentina

(Pergamon-Elsevier Science Ltd, Oxford, 2019)

TY  - JOUR
AU  - Perić, Mirjana
AU  - Rajković, Katarina
AU  - Milić-Lemić, Aleksandra
AU  - Živković, Rade
AU  - Arsić-Arsenijević, Valentina
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2470
AB  - Objective: The upward trend in using plant materials introduced essential oils (EOs) as a valuable, novel, bioactive antifungal agent and as an alternative to standard treatment protocol of denture stomatitis caused by Candida species. Therefore, the aim was to evaluate the antifungal activity of different EOs and to present the response surface methodology (RSM) and artificial neural network (ANN) as possible tools for optimizing and predicting EOs antifungal activity. Methods: Minimum inhibitory concentration (MIC) and Minimum fungicidal concentration (MFC) of the EOs against 3 species Candida spp. (C. albicans, C. tropicalis, C. glabrata) isolated in patients with DS were determined, together with optimization and prediction based on non-terpene and terpene content in EOs, using two mathematical models RSM and ANN-GA. Results: The highest concentrations of EO M. alternifolia inhibited (1.6-2.8 mu g/ml) and fungicided (3.5-6.0 mu g/ml) all three investigated Candida spp, while the lowest concentrations of EO C. Limon inhibited (0.2-0.5 mu g/ml) and fungicided (0.6-1.1 mu g/ml). Among the three types of Candida, C. glabrata was the most sensitive. The RSM modelling proved that MICs and MFCs statistically depend on non-terpene and terpene content in different EOs ( lt  0.0001). Both models showed that a citrus oil (EO C. Limon) with 89% content of terpenes and limonene as major constituent was more antifungal efficient. Conclusions: The investigated EOs showed a broad spectrum of anticandidal activity, also confirmed using the RSM and ANN-GA models. Since EOs can be cytotoxic in higher concentrations, models may be used for qualitative and quantitative dosage predictions of the antifungal activity of EOs.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Archives of Oral Biology
T1  - Development and validation of mathematical models for testing antifungal activity of different essential oils against Candida species
VL  - 98
SP  - 258
EP  - 264
DO  - 10.1016/j.archoralbio.2018.11.029
ER  - 
@article{
author = "Perić, Mirjana and Rajković, Katarina and Milić-Lemić, Aleksandra and Živković, Rade and Arsić-Arsenijević, Valentina",
year = "2019",
abstract = "Objective: The upward trend in using plant materials introduced essential oils (EOs) as a valuable, novel, bioactive antifungal agent and as an alternative to standard treatment protocol of denture stomatitis caused by Candida species. Therefore, the aim was to evaluate the antifungal activity of different EOs and to present the response surface methodology (RSM) and artificial neural network (ANN) as possible tools for optimizing and predicting EOs antifungal activity. Methods: Minimum inhibitory concentration (MIC) and Minimum fungicidal concentration (MFC) of the EOs against 3 species Candida spp. (C. albicans, C. tropicalis, C. glabrata) isolated in patients with DS were determined, together with optimization and prediction based on non-terpene and terpene content in EOs, using two mathematical models RSM and ANN-GA. Results: The highest concentrations of EO M. alternifolia inhibited (1.6-2.8 mu g/ml) and fungicided (3.5-6.0 mu g/ml) all three investigated Candida spp, while the lowest concentrations of EO C. Limon inhibited (0.2-0.5 mu g/ml) and fungicided (0.6-1.1 mu g/ml). Among the three types of Candida, C. glabrata was the most sensitive. The RSM modelling proved that MICs and MFCs statistically depend on non-terpene and terpene content in different EOs ( lt  0.0001). Both models showed that a citrus oil (EO C. Limon) with 89% content of terpenes and limonene as major constituent was more antifungal efficient. Conclusions: The investigated EOs showed a broad spectrum of anticandidal activity, also confirmed using the RSM and ANN-GA models. Since EOs can be cytotoxic in higher concentrations, models may be used for qualitative and quantitative dosage predictions of the antifungal activity of EOs.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Archives of Oral Biology",
title = "Development and validation of mathematical models for testing antifungal activity of different essential oils against Candida species",
volume = "98",
pages = "258-264",
doi = "10.1016/j.archoralbio.2018.11.029"
}
Perić, M., Rajković, K., Milić-Lemić, A., Živković, R.,& Arsić-Arsenijević, V.. (2019). Development and validation of mathematical models for testing antifungal activity of different essential oils against Candida species. in Archives of Oral Biology
Pergamon-Elsevier Science Ltd, Oxford., 98, 258-264.
https://doi.org/10.1016/j.archoralbio.2018.11.029
Perić M, Rajković K, Milić-Lemić A, Živković R, Arsić-Arsenijević V. Development and validation of mathematical models for testing antifungal activity of different essential oils against Candida species. in Archives of Oral Biology. 2019;98:258-264.
doi:10.1016/j.archoralbio.2018.11.029 .
Perić, Mirjana, Rajković, Katarina, Milić-Lemić, Aleksandra, Živković, Rade, Arsić-Arsenijević, Valentina, "Development and validation of mathematical models for testing antifungal activity of different essential oils against Candida species" in Archives of Oral Biology, 98 (2019):258-264,
https://doi.org/10.1016/j.archoralbio.2018.11.029 . .
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Laboratory-Based Investigation of Denture Sonication Method in Patients with Candida-Associated Denture Stomatitis

Perić, Mirjana; Radunović, Milena; Pekmezović, Marina; Marinković, Jelena; Živković, Rade; Arsić-Arsenijević, Valentina

(Wiley, Hoboken, 2019)

TY  - JOUR
AU  - Perić, Mirjana
AU  - Radunović, Milena
AU  - Pekmezović, Marina
AU  - Marinković, Jelena
AU  - Živković, Rade
AU  - Arsić-Arsenijević, Valentina
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2379
AB  - Purpose Denture stomatitis (DS) is a disease characterized by inflammation and erythema of the oral mucosa areas covered by the denture. Multifactorial etiological factors contribute to DS, but it seems that Candida plays the key role. The aim of the study was to evaluate the denture sonication method to: (i) increase the possibility of diagnosing patients with Candida-associated DS; (ii) detect and identify the mixed Candida spp., and (iii) determine the Candida colony forming units (CFU) and its possible relationship with DS severity, based on Newton's classification. Materials and Methods The cross-sectional study conducted at the Clinic for Dental Prosthetics, Belgrade (Serbia) from June 2013 to December 2014 enrolled edentulous patients with dentures (n = 250). Patients without DS (n = 20) were the control group (CG). The patients' data were collected, and patients with DS (study group/SG) were selected and divided into SG Candida+ and SG Candida-. Based on severity of DS, the SG patients were classified in 3 groups (Newton's classification). Four sampling methods were applied to detect patients with Candida-associated DS: mucosa swab, denture swab, oral rinse, and denture sonication method. The sensitivity and specificity of denture sonication method were shown using the receiver operating characteristic (ROC) curve and the area under the curve (AUC). Results In 97 (38.8%), out of 250 clinically examined patients, DS was diagnosed. In 82 (84.5%), out of 97 mycologically examined patients, Candida-associated DS was detected when denture sonication method was applied. Additionally, using the denture sonication method we observed: (i) the largest number of Candida positive patients compared to other sampling methods (p  lt  0.0001); (ii) the highest number of Candida CFU/ml (10(5)), and (iii) the possibility to detect mixed Candida cultures. The largest number of patients with Candida-associated DS showed type II (60%) DS, followed by type I (21%), and type III (19%) DS. Conclusion The denture sonication method is easy, accurate, and sensitive, and increases the possibility of diagnosing patients with Candida-associated DS. Additionally, yeast quantification, mixed Candida spp., and non-albicans Candida were detectable when cultivation on Candida CHROMagar was performed. It was not possible using conventional methods, such as swab or oral rinse.
PB  - Wiley, Hoboken
T2  - Journal of Prosthodontics-Implant Esthetic & Reconstructive Dentistry
T1  - Laboratory-Based Investigation of Denture Sonication Method in Patients with Candida-Associated Denture Stomatitis
VL  - 28
IS  - 5
SP  - 580
EP  - 586
DO  - 10.1111/jopr.12610
ER  - 
@article{
author = "Perić, Mirjana and Radunović, Milena and Pekmezović, Marina and Marinković, Jelena and Živković, Rade and Arsić-Arsenijević, Valentina",
year = "2019",
abstract = "Purpose Denture stomatitis (DS) is a disease characterized by inflammation and erythema of the oral mucosa areas covered by the denture. Multifactorial etiological factors contribute to DS, but it seems that Candida plays the key role. The aim of the study was to evaluate the denture sonication method to: (i) increase the possibility of diagnosing patients with Candida-associated DS; (ii) detect and identify the mixed Candida spp., and (iii) determine the Candida colony forming units (CFU) and its possible relationship with DS severity, based on Newton's classification. Materials and Methods The cross-sectional study conducted at the Clinic for Dental Prosthetics, Belgrade (Serbia) from June 2013 to December 2014 enrolled edentulous patients with dentures (n = 250). Patients without DS (n = 20) were the control group (CG). The patients' data were collected, and patients with DS (study group/SG) were selected and divided into SG Candida+ and SG Candida-. Based on severity of DS, the SG patients were classified in 3 groups (Newton's classification). Four sampling methods were applied to detect patients with Candida-associated DS: mucosa swab, denture swab, oral rinse, and denture sonication method. The sensitivity and specificity of denture sonication method were shown using the receiver operating characteristic (ROC) curve and the area under the curve (AUC). Results In 97 (38.8%), out of 250 clinically examined patients, DS was diagnosed. In 82 (84.5%), out of 97 mycologically examined patients, Candida-associated DS was detected when denture sonication method was applied. Additionally, using the denture sonication method we observed: (i) the largest number of Candida positive patients compared to other sampling methods (p  lt  0.0001); (ii) the highest number of Candida CFU/ml (10(5)), and (iii) the possibility to detect mixed Candida cultures. The largest number of patients with Candida-associated DS showed type II (60%) DS, followed by type I (21%), and type III (19%) DS. Conclusion The denture sonication method is easy, accurate, and sensitive, and increases the possibility of diagnosing patients with Candida-associated DS. Additionally, yeast quantification, mixed Candida spp., and non-albicans Candida were detectable when cultivation on Candida CHROMagar was performed. It was not possible using conventional methods, such as swab or oral rinse.",
publisher = "Wiley, Hoboken",
journal = "Journal of Prosthodontics-Implant Esthetic & Reconstructive Dentistry",
title = "Laboratory-Based Investigation of Denture Sonication Method in Patients with Candida-Associated Denture Stomatitis",
volume = "28",
number = "5",
pages = "580-586",
doi = "10.1111/jopr.12610"
}
Perić, M., Radunović, M., Pekmezović, M., Marinković, J., Živković, R.,& Arsić-Arsenijević, V.. (2019). Laboratory-Based Investigation of Denture Sonication Method in Patients with Candida-Associated Denture Stomatitis. in Journal of Prosthodontics-Implant Esthetic & Reconstructive Dentistry
Wiley, Hoboken., 28(5), 580-586.
https://doi.org/10.1111/jopr.12610
Perić M, Radunović M, Pekmezović M, Marinković J, Živković R, Arsić-Arsenijević V. Laboratory-Based Investigation of Denture Sonication Method in Patients with Candida-Associated Denture Stomatitis. in Journal of Prosthodontics-Implant Esthetic & Reconstructive Dentistry. 2019;28(5):580-586.
doi:10.1111/jopr.12610 .
Perić, Mirjana, Radunović, Milena, Pekmezović, Marina, Marinković, Jelena, Živković, Rade, Arsić-Arsenijević, Valentina, "Laboratory-Based Investigation of Denture Sonication Method in Patients with Candida-Associated Denture Stomatitis" in Journal of Prosthodontics-Implant Esthetic & Reconstructive Dentistry, 28, no. 5 (2019):580-586,
https://doi.org/10.1111/jopr.12610 . .
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The severity of denture stomatitis as related to risk factors and different Candida spp.

Perić, Mirjana; Živković, Rade; Milić-Lemić, Aleksandra; Radunović, Milena; Miličić, Biljana; Arsić-Arsenijević, Valentina

(Elsevier Science Inc, New York, 2018)

TY  - JOUR
AU  - Perić, Mirjana
AU  - Živković, Rade
AU  - Milić-Lemić, Aleksandra
AU  - Radunović, Milena
AU  - Miličić, Biljana
AU  - Arsić-Arsenijević, Valentina
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2298
AB  - Objectives. 1) To select patients with Candida-related denture stomatitis (DS) and to study possible risk factors associated with DS, 2) to evaluate the severity of DS according to Newton's classification, and 3) to investigate the association between the presence of non-albicans Candida spp. (NAC) or mixed Candida spp. and the severity of DS. Study Design. Eighty-two patients with Candida-positive DS have undergone 1) filling in the interview questionnaire, 2) clinical examination, and 3) microbiologic examination. Results. A total of 113 Candida spp. isolates were obtained from Candida-positive DS patients: C. albicans (as a single species) in 47/82 (57%) patients (study group A [SG_A]) and NAC/mixed Candida spp. in 35/82 (43%) patients (SG_B). Univariate logistic regression analysis showed that older age, longer age of the mandibular denture, and ex-smoker status were associated with SG_A. A multivariate model revealed no significant predictor of DS severity. Patients from SG_A were 3 times as likely to have DS type I, while patients from SG_B were 4.9 times as likely to have DS type III. Conclusions. Our results show the association between type III of DS (by Newton's classification) and the presence of NAC or mixed Candida spp. in denture wearers.
PB  - Elsevier Science Inc, New York
T2  - Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology
T1  - The severity of denture stomatitis as related to risk factors and different Candida spp.
VL  - 126
IS  - 1
SP  - 41
EP  - 47
DO  - 10.1016/j.oooo.2018.03.003
ER  - 
@article{
author = "Perić, Mirjana and Živković, Rade and Milić-Lemić, Aleksandra and Radunović, Milena and Miličić, Biljana and Arsić-Arsenijević, Valentina",
year = "2018",
abstract = "Objectives. 1) To select patients with Candida-related denture stomatitis (DS) and to study possible risk factors associated with DS, 2) to evaluate the severity of DS according to Newton's classification, and 3) to investigate the association between the presence of non-albicans Candida spp. (NAC) or mixed Candida spp. and the severity of DS. Study Design. Eighty-two patients with Candida-positive DS have undergone 1) filling in the interview questionnaire, 2) clinical examination, and 3) microbiologic examination. Results. A total of 113 Candida spp. isolates were obtained from Candida-positive DS patients: C. albicans (as a single species) in 47/82 (57%) patients (study group A [SG_A]) and NAC/mixed Candida spp. in 35/82 (43%) patients (SG_B). Univariate logistic regression analysis showed that older age, longer age of the mandibular denture, and ex-smoker status were associated with SG_A. A multivariate model revealed no significant predictor of DS severity. Patients from SG_A were 3 times as likely to have DS type I, while patients from SG_B were 4.9 times as likely to have DS type III. Conclusions. Our results show the association between type III of DS (by Newton's classification) and the presence of NAC or mixed Candida spp. in denture wearers.",
publisher = "Elsevier Science Inc, New York",
journal = "Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology",
title = "The severity of denture stomatitis as related to risk factors and different Candida spp.",
volume = "126",
number = "1",
pages = "41-47",
doi = "10.1016/j.oooo.2018.03.003"
}
Perić, M., Živković, R., Milić-Lemić, A., Radunović, M., Miličić, B.,& Arsić-Arsenijević, V.. (2018). The severity of denture stomatitis as related to risk factors and different Candida spp.. in Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology
Elsevier Science Inc, New York., 126(1), 41-47.
https://doi.org/10.1016/j.oooo.2018.03.003
Perić M, Živković R, Milić-Lemić A, Radunović M, Miličić B, Arsić-Arsenijević V. The severity of denture stomatitis as related to risk factors and different Candida spp.. in Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology. 2018;126(1):41-47.
doi:10.1016/j.oooo.2018.03.003 .
Perić, Mirjana, Živković, Rade, Milić-Lemić, Aleksandra, Radunović, Milena, Miličić, Biljana, Arsić-Arsenijević, Valentina, "The severity of denture stomatitis as related to risk factors and different Candida spp." in Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, 126, no. 1 (2018):41-47,
https://doi.org/10.1016/j.oooo.2018.03.003 . .
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Study toward resolving the controversy over the definition of allergic fungal rhinosinusitis

Barac, Aleksandra; Stevanović, Goran; Pekmezović, Marina; Rakočević, Zoran; Stošović, Rajica; Erović, Boban; Tomić-Spirić, Vesna

(Oxford Univ Press, Oxford, 2018)

TY  - JOUR
AU  - Barac, Aleksandra
AU  - Stevanović, Goran
AU  - Pekmezović, Marina
AU  - Rakočević, Zoran
AU  - Stošović, Rajica
AU  - Erović, Boban
AU  - Tomić-Spirić, Vesna
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2310
AB  - Dysbiosis of the microbiome on the airway mucosa leads to the development of chronic inflammatory and allergic disorders. The aim of this study was to consider the potential diagnostic criteria for allergic fungal rhinosinusitis (AFRS) and nonallergic fungal rhinosinusitis (FRS), and the role of fungal presence in an environment for the development of AFRS. In this study, 136 patients were divided into two groups: patients with positive specific immunoglobulin E (sIgE) and fungal finding (AFRS group), and patients with negative sIgE and positive fungal finding (FRS group). The study design included: anamnesis data, sIgE, eosinophil count and skin-prick test, rhinology and computerized tomography (CT) observation and mycological finding. Our results showed: (i) the prevalence in Serbia is: AFRS 1.3%, FRS 2.8%; (ii) 30.4% patients with sIgE+ had more often severe and recurrent chronic rhinosinusitis (CRS) (P = .005) and the presence of polyps (P = .025); (iii) 46.4% patients with sIgE+ had positive fungi on the sinonasal mucosa and were considered as AFRS; (iv) patients with AFRS had more frequent asthma (P = .024) and chronicity of CRS > 10 years (P = .000). The persistent fungal presence and prolonged duration of CRS could be a silent threat for the progression of inflammation and development of FRS. Lavage with hypertonic-NaCl should be included in the everyday hygiene routine in an effort to decrease fungal load and antigenic exposure. The presence of allergological parameters and better response to corticosteroid therapy in AFRS patients should be considered as crucial diagnostic criteria for AFRS.
PB  - Oxford Univ Press, Oxford
T2  - Medical Mycology
T1  - Study toward resolving the controversy over the definition of allergic fungal rhinosinusitis
VL  - 56
IS  - 2
SP  - 162
EP  - 171
DO  - 10.1093/mmy/myx032
ER  - 
@article{
author = "Barac, Aleksandra and Stevanović, Goran and Pekmezović, Marina and Rakočević, Zoran and Stošović, Rajica and Erović, Boban and Tomić-Spirić, Vesna",
year = "2018",
abstract = "Dysbiosis of the microbiome on the airway mucosa leads to the development of chronic inflammatory and allergic disorders. The aim of this study was to consider the potential diagnostic criteria for allergic fungal rhinosinusitis (AFRS) and nonallergic fungal rhinosinusitis (FRS), and the role of fungal presence in an environment for the development of AFRS. In this study, 136 patients were divided into two groups: patients with positive specific immunoglobulin E (sIgE) and fungal finding (AFRS group), and patients with negative sIgE and positive fungal finding (FRS group). The study design included: anamnesis data, sIgE, eosinophil count and skin-prick test, rhinology and computerized tomography (CT) observation and mycological finding. Our results showed: (i) the prevalence in Serbia is: AFRS 1.3%, FRS 2.8%; (ii) 30.4% patients with sIgE+ had more often severe and recurrent chronic rhinosinusitis (CRS) (P = .005) and the presence of polyps (P = .025); (iii) 46.4% patients with sIgE+ had positive fungi on the sinonasal mucosa and were considered as AFRS; (iv) patients with AFRS had more frequent asthma (P = .024) and chronicity of CRS > 10 years (P = .000). The persistent fungal presence and prolonged duration of CRS could be a silent threat for the progression of inflammation and development of FRS. Lavage with hypertonic-NaCl should be included in the everyday hygiene routine in an effort to decrease fungal load and antigenic exposure. The presence of allergological parameters and better response to corticosteroid therapy in AFRS patients should be considered as crucial diagnostic criteria for AFRS.",
publisher = "Oxford Univ Press, Oxford",
journal = "Medical Mycology",
title = "Study toward resolving the controversy over the definition of allergic fungal rhinosinusitis",
volume = "56",
number = "2",
pages = "162-171",
doi = "10.1093/mmy/myx032"
}
Barac, A., Stevanović, G., Pekmezović, M., Rakočević, Z., Stošović, R., Erović, B.,& Tomić-Spirić, V.. (2018). Study toward resolving the controversy over the definition of allergic fungal rhinosinusitis. in Medical Mycology
Oxford Univ Press, Oxford., 56(2), 162-171.
https://doi.org/10.1093/mmy/myx032
Barac A, Stevanović G, Pekmezović M, Rakočević Z, Stošović R, Erović B, Tomić-Spirić V. Study toward resolving the controversy over the definition of allergic fungal rhinosinusitis. in Medical Mycology. 2018;56(2):162-171.
doi:10.1093/mmy/myx032 .
Barac, Aleksandra, Stevanović, Goran, Pekmezović, Marina, Rakočević, Zoran, Stošović, Rajica, Erović, Boban, Tomić-Spirić, Vesna, "Study toward resolving the controversy over the definition of allergic fungal rhinosinusitis" in Medical Mycology, 56, no. 2 (2018):162-171,
https://doi.org/10.1093/mmy/myx032 . .
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Presence, species distribution, and density of Malassezia yeast in patients with seborrhoeic dermatitis - a community-based case-control study and review of literature

Barac, Aleksandra; Pekmezović, Marina; Milobratović, Danica; Otašević-Tasić, Suzana; Radunović, Milena; Arsić-Arsenijević, Valentina

(Wiley, Hoboken, 2015)

TY  - JOUR
AU  - Barac, Aleksandra
AU  - Pekmezović, Marina
AU  - Milobratović, Danica
AU  - Otašević-Tasić, Suzana
AU  - Radunović, Milena
AU  - Arsić-Arsenijević, Valentina
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2023
AB  - Malassezia yeast belongs to the normal cutaneous flora and under certain conditions it causes seborrhoeic dermatitis (SD). There is no culture-based study about the presence and density of the Malassezia in SD patients in Serbia. Aim was to show the presence, species distribution and density of Malassezia in patients with SD on lesional skin (LS) and non-lesional skin (NLS) and healthy controls (HC) and to compare data between Serbia and other countries. The study included 70 HC and 60 patients with SD in the study group (SG). Isolation, identification and examination of density of Malassezia colony-forming units from LS and NLS were performed. Malassezia was found more frequently in the SG than in HC, 90% and 60%, respectively (P lt 0.01). The most frequent isolates in SG on LS were M. slooffiae (26%), followed by M. globosa (17%) and M. sympodialis (17%). The yeast density was much higher on LS of SG than on NLS of SG or in the HC group (P lt 0.05). Higher density of Malassezia was shown on LS of SG than on NLS of SG and HC. M. slooffiae is the most prevalent species in SD patients in Serbia. This study demonstrated a positive relationship between severity of SD and presence of Malassezia spp.
PB  - Wiley, Hoboken
T2  - Mycoses
T1  - Presence, species distribution, and density of Malassezia yeast in patients with seborrhoeic dermatitis - a community-based case-control study and review of literature
VL  - 58
IS  - 2
SP  - 69
EP  - 75
DO  - 10.1111/myc.12276
ER  - 
@article{
author = "Barac, Aleksandra and Pekmezović, Marina and Milobratović, Danica and Otašević-Tasić, Suzana and Radunović, Milena and Arsić-Arsenijević, Valentina",
year = "2015",
abstract = "Malassezia yeast belongs to the normal cutaneous flora and under certain conditions it causes seborrhoeic dermatitis (SD). There is no culture-based study about the presence and density of the Malassezia in SD patients in Serbia. Aim was to show the presence, species distribution and density of Malassezia in patients with SD on lesional skin (LS) and non-lesional skin (NLS) and healthy controls (HC) and to compare data between Serbia and other countries. The study included 70 HC and 60 patients with SD in the study group (SG). Isolation, identification and examination of density of Malassezia colony-forming units from LS and NLS were performed. Malassezia was found more frequently in the SG than in HC, 90% and 60%, respectively (P lt 0.01). The most frequent isolates in SG on LS were M. slooffiae (26%), followed by M. globosa (17%) and M. sympodialis (17%). The yeast density was much higher on LS of SG than on NLS of SG or in the HC group (P lt 0.05). Higher density of Malassezia was shown on LS of SG than on NLS of SG and HC. M. slooffiae is the most prevalent species in SD patients in Serbia. This study demonstrated a positive relationship between severity of SD and presence of Malassezia spp.",
publisher = "Wiley, Hoboken",
journal = "Mycoses",
title = "Presence, species distribution, and density of Malassezia yeast in patients with seborrhoeic dermatitis - a community-based case-control study and review of literature",
volume = "58",
number = "2",
pages = "69-75",
doi = "10.1111/myc.12276"
}
Barac, A., Pekmezović, M., Milobratović, D., Otašević-Tasić, S., Radunović, M.,& Arsić-Arsenijević, V.. (2015). Presence, species distribution, and density of Malassezia yeast in patients with seborrhoeic dermatitis - a community-based case-control study and review of literature. in Mycoses
Wiley, Hoboken., 58(2), 69-75.
https://doi.org/10.1111/myc.12276
Barac A, Pekmezović M, Milobratović D, Otašević-Tasić S, Radunović M, Arsić-Arsenijević V. Presence, species distribution, and density of Malassezia yeast in patients with seborrhoeic dermatitis - a community-based case-control study and review of literature. in Mycoses. 2015;58(2):69-75.
doi:10.1111/myc.12276 .
Barac, Aleksandra, Pekmezović, Marina, Milobratović, Danica, Otašević-Tasić, Suzana, Radunović, Milena, Arsić-Arsenijević, Valentina, "Presence, species distribution, and density of Malassezia yeast in patients with seborrhoeic dermatitis - a community-based case-control study and review of literature" in Mycoses, 58, no. 2 (2015):69-75,
https://doi.org/10.1111/myc.12276 . .
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