TY  - JOUR
AU  - Grdović, Nevena
AU  - Rajić, Jovana
AU  - Matić-Petrović, Sanja
AU  - Dinić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Mihailović, Mirjana
AU  - Arambašić-Jovanović, Jelena
AU  - Tolić, Anja
AU  - Pucar, Ana
AU  - Milašin, Jelena
AU  - Vidaković, Melita
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2122
AB  - Objectives: CXCL12 is widely expressed, constitutive chemokine involved in tissue repair and regeneration, while the extent of its expression is important in various chronic inflammatory conditions. Involvement of DNA methylation in CXCL12 gene suppression (CXCL12) has been shown in malignancy and some autoimmune diseases. The aim of this study was to investigate whether the alterations in DNA methylation of CXCL12 are also involved in progression of periodontitis in combination with diabetes, as these chronic inflammatory conditions are strongly interrelated. Design: Study included 72 subjects divided in three groups: healthy control (C, n=21), periodontitis (P, n=29) and diabetes/periodontitis group (D/P, n=22). DNA extracted from epithelial cells obtained by sterile cotton swabs from buccal mucosa was subjected to methylation specific polymerase chain reaction (MSP) to obtain DNA methylation pattern of CXCL12 promoter. Results: CXCL12 promoter was predominantly unmethylated in all groups. However, increase in the frequency of the methylated form and increase in percent of methylation of CXCL12 promoter in periodontitis and diabetes/periodontitis group compared to control group were found, although without statistical significance. However, statistically significant increase in Tm of MSP products in diabetes/periodontitis group was observed. Correlation analysis revealed statistically significant relationship between the extent of DNA methylation of the CXCL12 promoter and periodontal parameters, as well as between DNA methylation of CXCL12 and glycosylated hemoglobin. Conclusion: Presented results suggest that chronic inflammation contributes to the change of CXCL12 DNA methylation in buccal cells and that DNA methylation profile of CXCL12 promoter plays important role in development and progression of periodontal disease.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Archives of Oral Biology
T1  - Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2
VL  - 72
SP  - 124
EP  - 133
DO  - 10.1016/j.archoralbio.2016.08.025
ER  - 

@article{
author = "Grdović, Nevena and Rajić, Jovana and Matić-Petrović, Sanja and Dinić, Svetlana and Uskoković, Aleksandra and Mihailović, Mirjana and Arambašić-Jovanović, Jelena and Tolić, Anja and Pucar, Ana and Milašin, Jelena and Vidaković, Melita",
year = "2016",
abstract = "Objectives: CXCL12 is widely expressed, constitutive chemokine involved in tissue repair and regeneration, while the extent of its expression is important in various chronic inflammatory conditions. Involvement of DNA methylation in CXCL12 gene suppression (CXCL12) has been shown in malignancy and some autoimmune diseases. The aim of this study was to investigate whether the alterations in DNA methylation of CXCL12 are also involved in progression of periodontitis in combination with diabetes, as these chronic inflammatory conditions are strongly interrelated. Design: Study included 72 subjects divided in three groups: healthy control (C, n=21), periodontitis (P, n=29) and diabetes/periodontitis group (D/P, n=22). DNA extracted from epithelial cells obtained by sterile cotton swabs from buccal mucosa was subjected to methylation specific polymerase chain reaction (MSP) to obtain DNA methylation pattern of CXCL12 promoter. Results: CXCL12 promoter was predominantly unmethylated in all groups. However, increase in the frequency of the methylated form and increase in percent of methylation of CXCL12 promoter in periodontitis and diabetes/periodontitis group compared to control group were found, although without statistical significance. However, statistically significant increase in Tm of MSP products in diabetes/periodontitis group was observed. Correlation analysis revealed statistically significant relationship between the extent of DNA methylation of the CXCL12 promoter and periodontal parameters, as well as between DNA methylation of CXCL12 and glycosylated hemoglobin. Conclusion: Presented results suggest that chronic inflammation contributes to the change of CXCL12 DNA methylation in buccal cells and that DNA methylation profile of CXCL12 promoter plays important role in development and progression of periodontal disease.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Archives of Oral Biology",
title = "Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2",
volume = "72",
pages = "124-133",
doi = "10.1016/j.archoralbio.2016.08.025"
}

Grdović, N., Rajić, J., Matić-Petrović, S., Dinić, S., Uskoković, A., Mihailović, M., Arambašić-Jovanović, J., Tolić, A., Pucar, A., Milašin, J.,& Vidaković, M.. (2016). Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2. in Archives of Oral Biology
Pergamon-Elsevier Science Ltd, Oxford., 72, 124-133.
https://doi.org/10.1016/j.archoralbio.2016.08.025

Grdović N, Rajić J, Matić-Petrović S, Dinić S, Uskoković A, Mihailović M, Arambašić-Jovanović J, Tolić A, Pucar A, Milašin J, Vidaković M. Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2. in Archives of Oral Biology. 2016;72:124-133.
doi:10.1016/j.archoralbio.2016.08.025 .

Grdović, Nevena, Rajić, Jovana, Matić-Petrović, Sanja, Dinić, Svetlana, Uskoković, Aleksandra, Mihailović, Mirjana, Arambašić-Jovanović, Jelena, Tolić, Anja, Pucar, Ana, Milašin, Jelena, Vidaković, Melita, "Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2" in Archives of Oral Biology, 72 (2016):124-133,
https://doi.org/10.1016/j.archoralbio.2016.08.025 . .