TY  - JOUR
AU  - Stojić, Vanja
AU  - Glišić, Branislav
AU  - Đukić, Ljiljana
AU  - Prokić, Bogomir
AU  - Janović, Aleksa
AU  - Stamenković, Zorana
AU  - Milutinović-Smiljanić, Sanja
AU  - Danilović, Vesna
AU  - Brković, Božidar
AU  - Roganović, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2512
AB  - Objective: We aimed to investigate alteration in cellular signaling mediated by vascular endothelial growth factor (VEGF) and parameters of oxidative stress/nitric oxide generation, superoxide dismutase (SOD) and neuronal nitric oxide synthase (nNOS), underlying altered functional mechanical loading of TMJ (temporomandibular joint) during lateral mandibular deviation. Design: Thirty-eight 5-week-old male Wistar rats were divided into experimental group, which received acrylic resin appliance that shifted mandible to the left during closure, and control group. Computed tomography and histomorphometry were used for condyle analyses, while samples of condyle, synovial membrane and m. masseter were analyzed with enzyme-linked immunosorbent assay and spectrophotometry to determine VEGF and nNOS protein concentrations, and SOD activity. Results: Experimental group of rats developed smaller and asymmetrical mandibles. Less of new bone and cartilage formation and larger bone marrow cavities area were found in the experimental group. Higher VEGF expression in condyle and m. masseter as well as higher nNOS expression in m. masseter and synovial membrane were found in the experimental compared to the control group. Alteration of SOD activity was found in m. masseter and synovial membrane in the experimental group. Conclusions: Lateral mandibular deviation induces mandibular and condylar morphological changes as well as significant cellular signaling alterations in condyle, synovial membrane and masticatory muscle. Cellular VEGF protein overexpression and oxidative stress/nitric oxide disbalance could be the mechanisms underlying unbalanced functional TMJ loading due to mandibular deviation.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Archives of Oral Biology
T1  - Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle
VL  - 110
DO  - 10.1016/j.archoralbio.2019.104599
ER  - 

@article{
author = "Stojić, Vanja and Glišić, Branislav and Đukić, Ljiljana and Prokić, Bogomir and Janović, Aleksa and Stamenković, Zorana and Milutinović-Smiljanić, Sanja and Danilović, Vesna and Brković, Božidar and Roganović, Jelena",
year = "2020",
abstract = "Objective: We aimed to investigate alteration in cellular signaling mediated by vascular endothelial growth factor (VEGF) and parameters of oxidative stress/nitric oxide generation, superoxide dismutase (SOD) and neuronal nitric oxide synthase (nNOS), underlying altered functional mechanical loading of TMJ (temporomandibular joint) during lateral mandibular deviation. Design: Thirty-eight 5-week-old male Wistar rats were divided into experimental group, which received acrylic resin appliance that shifted mandible to the left during closure, and control group. Computed tomography and histomorphometry were used for condyle analyses, while samples of condyle, synovial membrane and m. masseter were analyzed with enzyme-linked immunosorbent assay and spectrophotometry to determine VEGF and nNOS protein concentrations, and SOD activity. Results: Experimental group of rats developed smaller and asymmetrical mandibles. Less of new bone and cartilage formation and larger bone marrow cavities area were found in the experimental group. Higher VEGF expression in condyle and m. masseter as well as higher nNOS expression in m. masseter and synovial membrane were found in the experimental compared to the control group. Alteration of SOD activity was found in m. masseter and synovial membrane in the experimental group. Conclusions: Lateral mandibular deviation induces mandibular and condylar morphological changes as well as significant cellular signaling alterations in condyle, synovial membrane and masticatory muscle. Cellular VEGF protein overexpression and oxidative stress/nitric oxide disbalance could be the mechanisms underlying unbalanced functional TMJ loading due to mandibular deviation.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Archives of Oral Biology",
title = "Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle",
volume = "110",
doi = "10.1016/j.archoralbio.2019.104599"
}

Stojić, V., Glišić, B., Đukić, L., Prokić, B., Janović, A., Stamenković, Z., Milutinović-Smiljanić, S., Danilović, V., Brković, B.,& Roganović, J.. (2020). Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle. in Archives of Oral Biology
Pergamon-Elsevier Science Ltd, Oxford., 110.
https://doi.org/10.1016/j.archoralbio.2019.104599

Stojić V, Glišić B, Đukić L, Prokić B, Janović A, Stamenković Z, Milutinović-Smiljanić S, Danilović V, Brković B, Roganović J. Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle. in Archives of Oral Biology. 2020;110.
doi:10.1016/j.archoralbio.2019.104599 .

Stojić, Vanja, Glišić, Branislav, Đukić, Ljiljana, Prokić, Bogomir, Janović, Aleksa, Stamenković, Zorana, Milutinović-Smiljanić, Sanja, Danilović, Vesna, Brković, Božidar, Roganović, Jelena, "Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle" in Archives of Oral Biology, 110 (2020),
https://doi.org/10.1016/j.archoralbio.2019.104599 . .

TY  - JOUR
AU  - Dželetović, Bojan
AU  - Aleksić, Nikola
AU  - Radak, Đorđe
AU  - Stratimirović, Đorđe
AU  - Đukić, Ljiljana
AU  - Stojić, Dragica
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2499
AB  - Introduction: Age-related changes of dental pulp tissue and atherosclerosis of carotid arteries as its feeding arteries could influence the functionality of pulpal circulation. The objective of our study was to evaluate the effect of aging (physiological process) and carotid bifurcation atherosclerosis (pathologic process) on the pulpal microcirculatory system using multifractal analysis of the laser Doppler flowmetry signal. Methods: Three groups of 10 subjects were enrolled in the study: the young group (healthy subjects, 20-25 years), the middle-aged group (healthy subjects, 50-60 years), and the clinical group (subjects with carotid bifurcation atherosclerosis, 50-60 years). Pulpal blood flow (PBF) signals recorded by laser Doppler flowmetry were assessed by multifractal analysis that estimates Holder exponents of the signal. PBF levels, the average mean values, and the range of Holder exponents were obtained. Results: PBF levels were significantly higher in the young group compared with the middle-aged and clinical groups, and the difference between the middle-aged and clinical groups was not statistically significant. The range of the Holder exponents was narrower in the middle-aged and clinical groups than in the young group and narrower in the clinical group than in the middle-aged group. The average mean value of Holder exponents was significantly higher in the young group than in the middle-aged and clinical groups, whereas there was no significant difference between the middle-aged and clinical groups. Conclusions: Our study investigating the multifractality of the PBF signal showed that the aging process and carotid atherosclerosis could affect the complex structure of PBF oscillations and contribute to a better understanding of pulpal hemodynamics.
PB  - Elsevier Science Inc, New York
T2  - Journal of Endodontics
T1  - Effect of Aging and Carotid Atherosclerosis on Multifractality of Dental Pulp Blood Flow Oscillations
VL  - 46
IS  - 3
SP  - 358
EP  - 363
DO  - 10.1016/j.joen.2019.12.008
ER  - 

@article{
author = "Dželetović, Bojan and Aleksić, Nikola and Radak, Đorđe and Stratimirović, Đorđe and Đukić, Ljiljana and Stojić, Dragica",
year = "2020",
abstract = "Introduction: Age-related changes of dental pulp tissue and atherosclerosis of carotid arteries as its feeding arteries could influence the functionality of pulpal circulation. The objective of our study was to evaluate the effect of aging (physiological process) and carotid bifurcation atherosclerosis (pathologic process) on the pulpal microcirculatory system using multifractal analysis of the laser Doppler flowmetry signal. Methods: Three groups of 10 subjects were enrolled in the study: the young group (healthy subjects, 20-25 years), the middle-aged group (healthy subjects, 50-60 years), and the clinical group (subjects with carotid bifurcation atherosclerosis, 50-60 years). Pulpal blood flow (PBF) signals recorded by laser Doppler flowmetry were assessed by multifractal analysis that estimates Holder exponents of the signal. PBF levels, the average mean values, and the range of Holder exponents were obtained. Results: PBF levels were significantly higher in the young group compared with the middle-aged and clinical groups, and the difference between the middle-aged and clinical groups was not statistically significant. The range of the Holder exponents was narrower in the middle-aged and clinical groups than in the young group and narrower in the clinical group than in the middle-aged group. The average mean value of Holder exponents was significantly higher in the young group than in the middle-aged and clinical groups, whereas there was no significant difference between the middle-aged and clinical groups. Conclusions: Our study investigating the multifractality of the PBF signal showed that the aging process and carotid atherosclerosis could affect the complex structure of PBF oscillations and contribute to a better understanding of pulpal hemodynamics.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Endodontics",
title = "Effect of Aging and Carotid Atherosclerosis on Multifractality of Dental Pulp Blood Flow Oscillations",
volume = "46",
number = "3",
pages = "358-363",
doi = "10.1016/j.joen.2019.12.008"
}

Dželetović, B., Aleksić, N., Radak, Đ., Stratimirović, Đ., Đukić, L.,& Stojić, D.. (2020). Effect of Aging and Carotid Atherosclerosis on Multifractality of Dental Pulp Blood Flow Oscillations. in Journal of Endodontics
Elsevier Science Inc, New York., 46(3), 358-363.
https://doi.org/10.1016/j.joen.2019.12.008

Dželetović B, Aleksić N, Radak Đ, Stratimirović Đ, Đukić L, Stojić D. Effect of Aging and Carotid Atherosclerosis on Multifractality of Dental Pulp Blood Flow Oscillations. in Journal of Endodontics. 2020;46(3):358-363.
doi:10.1016/j.joen.2019.12.008 .

Dželetović, Bojan, Aleksić, Nikola, Radak, Đorđe, Stratimirović, Đorđe, Đukić, Ljiljana, Stojić, Dragica, "Effect of Aging and Carotid Atherosclerosis on Multifractality of Dental Pulp Blood Flow Oscillations" in Journal of Endodontics, 46, no. 3 (2020):358-363,
https://doi.org/10.1016/j.joen.2019.12.008 . .

TY  - JOUR
AU  - Dželetović, Bojan
AU  - Stratimirović, Đorđe
AU  - Stojić, Dragica
AU  - Đukić, Ljiljana
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1049
AB  - Aim To investigate the effect of ageing on control mechanisms of pulpal microcirculation using wavelet analysis and to calculate linear and nonlinear parameters of blood flow oscillations, in a healthy general population. Methodology Pulpal blood flow (PBF) oscillations were recorded on right maxillary central incisors using laser Doppler Flowmeter (PeriFlux PF 5001, Perimed, Jarfalla, Sweden) on a group of 10 young participants (20-25 years) and a group of 10 older adults (60-70 years). In total, 20 recordings were obtained for at least 20 min (one recording on one tooth per subject). Using wavelet spectral analysis, the amplitude and power were calculated as a linear and Hurst exponent as a nonlinear parameter of PBF oscillations. Differences between the two groups were assessed with the independent Student t-test. Results Mean PBF levels were significantly lower (P = 0.024) in older adults than in young participants. Relative amplitudes and powers corresponding to the myogenic (P = 0.046, P  lt  0.001, respectively) and neurogenic activity (P = 0.04, P = 0.01, respectively) were significantly higher, whereas values corresponding to the endothelial function (P = 0.04, P = 0.01, respectively) were significantly lower in older adults than in young participants. Hurst exponents of the total spectrum, myogenic and endothelial component (P  lt  0.001, P = 0.02, P  lt  0.001, respectively) of PBF oscillations were significantly lower in older adults in comparison to young participants. Conclusions At the level of pulpal microcirculation, ageing was associated with altered blood flow levels, the contribution of different control mechanisms to blood flow oscillations as well as the interaction of vascular smooth muscle and endothelium. Described changes of pulpal haemodynamics contribute to a better understanding of physiological behaviour and decreased adaptability of aged dental pulp to pathological stimuli.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Linear and nonlinear analysis of dental pulp blood flow oscillations in ageing
DO  - 10.1111/iej.13306
ER  - 

@article{
author = "Dželetović, Bojan and Stratimirović, Đorđe and Stojić, Dragica and Đukić, Ljiljana",
year = "2020",
abstract = "Aim To investigate the effect of ageing on control mechanisms of pulpal microcirculation using wavelet analysis and to calculate linear and nonlinear parameters of blood flow oscillations, in a healthy general population. Methodology Pulpal blood flow (PBF) oscillations were recorded on right maxillary central incisors using laser Doppler Flowmeter (PeriFlux PF 5001, Perimed, Jarfalla, Sweden) on a group of 10 young participants (20-25 years) and a group of 10 older adults (60-70 years). In total, 20 recordings were obtained for at least 20 min (one recording on one tooth per subject). Using wavelet spectral analysis, the amplitude and power were calculated as a linear and Hurst exponent as a nonlinear parameter of PBF oscillations. Differences between the two groups were assessed with the independent Student t-test. Results Mean PBF levels were significantly lower (P = 0.024) in older adults than in young participants. Relative amplitudes and powers corresponding to the myogenic (P = 0.046, P  lt  0.001, respectively) and neurogenic activity (P = 0.04, P = 0.01, respectively) were significantly higher, whereas values corresponding to the endothelial function (P = 0.04, P = 0.01, respectively) were significantly lower in older adults than in young participants. Hurst exponents of the total spectrum, myogenic and endothelial component (P  lt  0.001, P = 0.02, P  lt  0.001, respectively) of PBF oscillations were significantly lower in older adults in comparison to young participants. Conclusions At the level of pulpal microcirculation, ageing was associated with altered blood flow levels, the contribution of different control mechanisms to blood flow oscillations as well as the interaction of vascular smooth muscle and endothelium. Described changes of pulpal haemodynamics contribute to a better understanding of physiological behaviour and decreased adaptability of aged dental pulp to pathological stimuli.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Linear and nonlinear analysis of dental pulp blood flow oscillations in ageing",
doi = "10.1111/iej.13306"
}

Dželetović, B., Stratimirović, Đ., Stojić, D.,& Đukić, L.. (2020). Linear and nonlinear analysis of dental pulp blood flow oscillations in ageing. in International Endodontic Journal
Wiley, Hoboken..
https://doi.org/10.1111/iej.13306

Dželetović B, Stratimirović Đ, Stojić D, Đukić L. Linear and nonlinear analysis of dental pulp blood flow oscillations in ageing. in International Endodontic Journal. 2020;.
doi:10.1111/iej.13306 .

Dželetović, Bojan, Stratimirović, Đorđe, Stojić, Dragica, Đukić, Ljiljana, "Linear and nonlinear analysis of dental pulp blood flow oscillations in ageing" in International Endodontic Journal (2020),
https://doi.org/10.1111/iej.13306 . .

TY  - JOUR
AU  - Ilić, Jugoslav
AU  - Radović, Katarina
AU  - Brković, Božidar
AU  - Vasić, Jugoslav
AU  - Roganović, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2588
AB  - Introduction/Objective. We aimed to investigate the effects of diabetes mellitus (DM) on rat dental pulp repair by measuring time-dependent changes in expressions of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP 2) following direct pulp capping. Methods. Two groups, each of 20 Wistar rats, received either streptozotocin (for DM induction) or the same volume of sterile saline. A week later, the pulp of maxillary and mandibular right incisors in diabetic and non-diabetic groups were exposed and capped with calcium hydroxide in order to provoke reparative response. The levels of VEGF and BMP 2 were determined in the pulp tissue lysates one and seven days after the pulp capping, using enzyme-linked immunosorbent assays. Results. Diabetic state per se increased VEGF level, with a peak at first day after the pulp capping (19.3 ± 0.9 pg/mg, p < 0.001), but did not affect BMP 2 levels. Significant increase of BMP 2 expression was noticed on the seventh day in capped pulp, but only in diabetic rats (16.7 ± 1 pg/mg, p = 0.001). Positive correlation between VEGF and BMP 2 was found on the seventh day following capping, only in diabetic pulp (r = 0.905, p = 0.003). Conclusion. Diabetes-induced increase in VEGF expression reflects changes in the inflammatory phase of pulp repair in DM. Increase in BMP 2 expression suggest that stimulating effect of calcium hydroxide appears seven days after diabetic pulp capping.
AB  - Uvod/Cilj Cilj ove studije bio je da se ispita efekat dijabetesa melitusa na reparaciju zubne pulpe pacova utvrđivanjem vremenski zavisnih promena u ekspresiji vaskularnog endotelnog faktora rasta (VEGF) i kostnog morfogenetskog proteina 2 (BMP 2) posle direktnog prekrivanja pulpe. Metode Istraživanje je sprovedeno na pacovima soja vistar, podeljenim u dve grupe od po 20 životinja, pri čemu je jedna grupa dobila streptozotocin (za indukciju dijabetesa melitusa), a druga sterilni fiziološki rastvor u istoj zapremini. Posle nedelju dana pulpe maksilarnih i mandibularnih donjih inciziva kod dijabetičnih i nedijabetičnih životinja su eksponirane i odmah zatim prekrivene kalcijum-hidroksidom da bi se izazvao reparatorni odgovor. Nivoi VEGF i BMP 2 su utvrđivani u lizatima pulpnog tkiva, prvog i sedmog dana posle direktnog prekrivanja imunoenzimskim testom ELISA. Rezultati Dijabetično stanje je dovelo do rasta nivoa VEGF, sa maksimumom utvrđenim prvog dana posle prekrivanja pulpe (19,3 ± 0,9 pg/mg, p < 0,001), ali nije uticalo na nivoe BMP 2. Značajan porast BMP 2 je utvrđen sedmog dana posle prekrivanja pulpe, ali samo kod dijabetičnih pacova (16,7 ± 1 pg/mg, p = 0,001). Kod ovih životinja u istom periodu posle prekrivanja nađena je pozitivna korelacija između nivoa VEGF i BMP 2 (r = 0,905, p = 0,003). Zaključak Dijabetesom indukovan porast ekspresije VEGF ukazuje na promene u inflamatornoj fazi pulpne reparacije. Porast ekspresije BMP 2 ukazuje da se stimulativan reparatorni efekat kalcijum-hidroksida javlja sedmog dana posle prekrivanja dijabetične pulpe.
PB  - Beograd: Srpsko lekarsko društvo
T2  - Srpski arhiv za celokupno lekarstvo
T1  - The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2
T1  - Reparacija dijabetične zubne pulpe – uloga vaskularnog endotelnog faktora rasta i kostnog morfogenetskog proteina 2
VL  - 148
IS  - 11-12
SP  - 673
EP  - 678
DO  - 10.2298/SARH200228057I
ER  - 

@article{
author = "Ilić, Jugoslav and Radović, Katarina and Brković, Božidar and Vasić, Jugoslav and Roganović, Jelena",
year = "2020",
abstract = "Introduction/Objective. We aimed to investigate the effects of diabetes mellitus (DM) on rat dental pulp repair by measuring time-dependent changes in expressions of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP 2) following direct pulp capping. Methods. Two groups, each of 20 Wistar rats, received either streptozotocin (for DM induction) or the same volume of sterile saline. A week later, the pulp of maxillary and mandibular right incisors in diabetic and non-diabetic groups were exposed and capped with calcium hydroxide in order to provoke reparative response. The levels of VEGF and BMP 2 were determined in the pulp tissue lysates one and seven days after the pulp capping, using enzyme-linked immunosorbent assays. Results. Diabetic state per se increased VEGF level, with a peak at first day after the pulp capping (19.3 ± 0.9 pg/mg, p < 0.001), but did not affect BMP 2 levels. Significant increase of BMP 2 expression was noticed on the seventh day in capped pulp, but only in diabetic rats (16.7 ± 1 pg/mg, p = 0.001). Positive correlation between VEGF and BMP 2 was found on the seventh day following capping, only in diabetic pulp (r = 0.905, p = 0.003). Conclusion. Diabetes-induced increase in VEGF expression reflects changes in the inflammatory phase of pulp repair in DM. Increase in BMP 2 expression suggest that stimulating effect of calcium hydroxide appears seven days after diabetic pulp capping., Uvod/Cilj Cilj ove studije bio je da se ispita efekat dijabetesa melitusa na reparaciju zubne pulpe pacova utvrđivanjem vremenski zavisnih promena u ekspresiji vaskularnog endotelnog faktora rasta (VEGF) i kostnog morfogenetskog proteina 2 (BMP 2) posle direktnog prekrivanja pulpe. Metode Istraživanje je sprovedeno na pacovima soja vistar, podeljenim u dve grupe od po 20 životinja, pri čemu je jedna grupa dobila streptozotocin (za indukciju dijabetesa melitusa), a druga sterilni fiziološki rastvor u istoj zapremini. Posle nedelju dana pulpe maksilarnih i mandibularnih donjih inciziva kod dijabetičnih i nedijabetičnih životinja su eksponirane i odmah zatim prekrivene kalcijum-hidroksidom da bi se izazvao reparatorni odgovor. Nivoi VEGF i BMP 2 su utvrđivani u lizatima pulpnog tkiva, prvog i sedmog dana posle direktnog prekrivanja imunoenzimskim testom ELISA. Rezultati Dijabetično stanje je dovelo do rasta nivoa VEGF, sa maksimumom utvrđenim prvog dana posle prekrivanja pulpe (19,3 ± 0,9 pg/mg, p < 0,001), ali nije uticalo na nivoe BMP 2. Značajan porast BMP 2 je utvrđen sedmog dana posle prekrivanja pulpe, ali samo kod dijabetičnih pacova (16,7 ± 1 pg/mg, p = 0,001). Kod ovih životinja u istom periodu posle prekrivanja nađena je pozitivna korelacija između nivoa VEGF i BMP 2 (r = 0,905, p = 0,003). Zaključak Dijabetesom indukovan porast ekspresije VEGF ukazuje na promene u inflamatornoj fazi pulpne reparacije. Porast ekspresije BMP 2 ukazuje da se stimulativan reparatorni efekat kalcijum-hidroksida javlja sedmog dana posle prekrivanja dijabetične pulpe.",
publisher = "Beograd: Srpsko lekarsko društvo",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2, Reparacija dijabetične zubne pulpe – uloga vaskularnog endotelnog faktora rasta i kostnog morfogenetskog proteina 2",
volume = "148",
number = "11-12",
pages = "673-678",
doi = "10.2298/SARH200228057I"
}

Ilić, J., Radović, K., Brković, B., Vasić, J.,& Roganović, J.. (2020). The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2. in Srpski arhiv za celokupno lekarstvo
Beograd: Srpsko lekarsko društvo., 148(11-12), 673-678.
https://doi.org/10.2298/SARH200228057I

Ilić J, Radović K, Brković B, Vasić J, Roganović J. The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2. in Srpski arhiv za celokupno lekarstvo. 2020;148(11-12):673-678.
doi:10.2298/SARH200228057I .

Ilić, Jugoslav, Radović, Katarina, Brković, Božidar, Vasić, Jugoslav, Roganović, Jelena, "The diabetic dental pulp repair: involvement of vascular endothelial growth factor and bone morphogenetic protein 2" in Srpski arhiv za celokupno lekarstvo, 148, no. 11-12 (2020):673-678,
https://doi.org/10.2298/SARH200228057I . .

TY  - JOUR
AU  - Jurišić, Tamara
AU  - Milić, Marija
AU  - Todorović, Vladimir S.
AU  - Živković, Marko
AU  - Jurišić, Milan
AU  - Milić-Lemić, Aleksandra
AU  - Tihaček-Šojić, Ljiljana
AU  - Brković, Božidar
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2497
AB  - Background/Aim. Alveolar ridge dimensional alterations following tooth extraction in the anterior maxilla often result in an inadequate bone volume for a correct implant placement. In order to obtain optimal bone volume various bone graft substitutes have become commercially available and widely used for socket grafting. The aim of this study was to examine and compare long-term clinical outcomes of dental implant therapy in the maxillary esthetic zone, after socket grafting with beta-tricalcium phosphate (TCP) combined with collagen type I, either with or without barrier membrane and flap surgery, after a 12-month follow-up. Methods. Twenty healthy patients were allocated to either C group (beta-TCP and type I collagen without mucoperiosteal flap coverage) or C+M group (beta-TCP and type I collagen barrier membrane with mucoperiosteal flap coverage). Following clinical parameters were assessed: implant stability (evaluated by a resonance frequency analysis - RFA), periimplant soft tissue stability (sulcus bleeding index - SBI, Mombelli sulcus bleeding index - MBI, periimplant sulcus depth, keratinized gingiva width, gingival level) and marginal bone level at the retroalveolar radiograms. Results. Within C+M group, RFA values significantly increased 12 weeks after implant installation compared to primary RFA values. Comparison between investigated groups showed a significantly reduced keratinized gingiva width in the C+M group compared to the C group after 3, 6, 9 and 12 months. Comparison between groups revealed significantly lower gingival level values in the C+M group at 9th and 12th month when compared to the C group. Conclusion. Implant treatment in the anterior maxilla could be effective when using a 9 months alveolar ridge preservation healing with combined treatment with beta-tricalcium phosphate and type I collagen, with regard to the peri-implant soft and hard tissue stability.
AB  - Uvod/Cilj. Posle ekstrakcije zuba, dimenzionalne promene alveolarnog grebena u estetskoj regiji gornje vilice za posledicu često imaju nedovoljnu količinu kosti za ugradnju zubnih implanata. U vezi sa tim, primenjuju se različiti koštani zamenici sa ciljem očuvanja dimenzija alveolarnog grebena posle ekstrakcije zuba. Cilj rada bio je da se, posle prezervacije alveolarnog grebena beta-trikalcijum fosfatom (TCP) sa kolagenom tip 1, sa barijernom membranom i mukoperiostalnim režnjem i bez nje, ispitaju i uporede klinički ishodi zarastanja posle ugradnje zubnih implanata u estetskoj regiji gornje vilice, tokom jednogodišnjeg perioda praćenja. Metode. Dvadeset zdravih bolesnika podeljeno je u dve grupe: C (beta TCP/kolagen tip 1 bez barijerne membrane i mukoperiostalnog režnja) i C+M (beta TCP/kolagen tip 1 sa barijernom membranom i mukoperiostalnim režnjem). Praćeni su uobičajeni klinički parametri ishoda terapije: implantna stabilnost (analiza rezonantne frekvence), stanje mekih tkiva (indeks krvarenja, plak indeks, širina pripojne mukoze, recesija gingive) i nivo periimplantnog koštanog tkiva na retroalveolarnom radiogramu. Rezultati. U C+M grupi, implantna stabilnost posle 12 nedelja bila je značajno veća u odnosu na primarnu stabilnost. U C+M grupi, širina keratinizovane gingive bila je značajno manja posle 3, 6, 9 i 12 meseci u odnosu na C grupu. Recesija gingive bila je značajno veća u C+M grupi u odnosu na C grupu posle 9 i 12 meseci. Zaključak. Razmatrajući stabilnost mekog i tvrdog periimplantnog tkiva, terapija zubnim implantima može biti uspešna prilikom ugradnje u estetskoj regiji gornje vilice.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Peri-implant soft and hard tissue condition after alveolar ridge preservation with beta-tricalcium phosphate/type I collagen in the maxillary esthetic zone: A 1-year follow-up study
T1  - Stanje tvrdog i mekog periimplantnog tkiva u estetskoj regiji gornje vilice posle prezervacije alveolarnog grebena beta-trikalcijum fosfatom sa kolagenom tip I - studija sa jednogodišnjim periodom praćenja
VL  - 77
IS  - 1
SP  - 22
EP  - 28
DO  - 10.2298/VSP180128047J
ER  - 

@article{
author = "Jurišić, Tamara and Milić, Marija and Todorović, Vladimir S. and Živković, Marko and Jurišić, Milan and Milić-Lemić, Aleksandra and Tihaček-Šojić, Ljiljana and Brković, Božidar",
year = "2020",
abstract = "Background/Aim. Alveolar ridge dimensional alterations following tooth extraction in the anterior maxilla often result in an inadequate bone volume for a correct implant placement. In order to obtain optimal bone volume various bone graft substitutes have become commercially available and widely used for socket grafting. The aim of this study was to examine and compare long-term clinical outcomes of dental implant therapy in the maxillary esthetic zone, after socket grafting with beta-tricalcium phosphate (TCP) combined with collagen type I, either with or without barrier membrane and flap surgery, after a 12-month follow-up. Methods. Twenty healthy patients were allocated to either C group (beta-TCP and type I collagen without mucoperiosteal flap coverage) or C+M group (beta-TCP and type I collagen barrier membrane with mucoperiosteal flap coverage). Following clinical parameters were assessed: implant stability (evaluated by a resonance frequency analysis - RFA), periimplant soft tissue stability (sulcus bleeding index - SBI, Mombelli sulcus bleeding index - MBI, periimplant sulcus depth, keratinized gingiva width, gingival level) and marginal bone level at the retroalveolar radiograms. Results. Within C+M group, RFA values significantly increased 12 weeks after implant installation compared to primary RFA values. Comparison between investigated groups showed a significantly reduced keratinized gingiva width in the C+M group compared to the C group after 3, 6, 9 and 12 months. Comparison between groups revealed significantly lower gingival level values in the C+M group at 9th and 12th month when compared to the C group. Conclusion. Implant treatment in the anterior maxilla could be effective when using a 9 months alveolar ridge preservation healing with combined treatment with beta-tricalcium phosphate and type I collagen, with regard to the peri-implant soft and hard tissue stability., Uvod/Cilj. Posle ekstrakcije zuba, dimenzionalne promene alveolarnog grebena u estetskoj regiji gornje vilice za posledicu često imaju nedovoljnu količinu kosti za ugradnju zubnih implanata. U vezi sa tim, primenjuju se različiti koštani zamenici sa ciljem očuvanja dimenzija alveolarnog grebena posle ekstrakcije zuba. Cilj rada bio je da se, posle prezervacije alveolarnog grebena beta-trikalcijum fosfatom (TCP) sa kolagenom tip 1, sa barijernom membranom i mukoperiostalnim režnjem i bez nje, ispitaju i uporede klinički ishodi zarastanja posle ugradnje zubnih implanata u estetskoj regiji gornje vilice, tokom jednogodišnjeg perioda praćenja. Metode. Dvadeset zdravih bolesnika podeljeno je u dve grupe: C (beta TCP/kolagen tip 1 bez barijerne membrane i mukoperiostalnog režnja) i C+M (beta TCP/kolagen tip 1 sa barijernom membranom i mukoperiostalnim režnjem). Praćeni su uobičajeni klinički parametri ishoda terapije: implantna stabilnost (analiza rezonantne frekvence), stanje mekih tkiva (indeks krvarenja, plak indeks, širina pripojne mukoze, recesija gingive) i nivo periimplantnog koštanog tkiva na retroalveolarnom radiogramu. Rezultati. U C+M grupi, implantna stabilnost posle 12 nedelja bila je značajno veća u odnosu na primarnu stabilnost. U C+M grupi, širina keratinizovane gingive bila je značajno manja posle 3, 6, 9 i 12 meseci u odnosu na C grupu. Recesija gingive bila je značajno veća u C+M grupi u odnosu na C grupu posle 9 i 12 meseci. Zaključak. Razmatrajući stabilnost mekog i tvrdog periimplantnog tkiva, terapija zubnim implantima može biti uspešna prilikom ugradnje u estetskoj regiji gornje vilice.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Peri-implant soft and hard tissue condition after alveolar ridge preservation with beta-tricalcium phosphate/type I collagen in the maxillary esthetic zone: A 1-year follow-up study, Stanje tvrdog i mekog periimplantnog tkiva u estetskoj regiji gornje vilice posle prezervacije alveolarnog grebena beta-trikalcijum fosfatom sa kolagenom tip I - studija sa jednogodišnjim periodom praćenja",
volume = "77",
number = "1",
pages = "22-28",
doi = "10.2298/VSP180128047J"
}

Jurišić, T., Milić, M., Todorović, V. S., Živković, M., Jurišić, M., Milić-Lemić, A., Tihaček-Šojić, L.,& Brković, B.. (2020). Peri-implant soft and hard tissue condition after alveolar ridge preservation with beta-tricalcium phosphate/type I collagen in the maxillary esthetic zone: A 1-year follow-up study. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 77(1), 22-28.
https://doi.org/10.2298/VSP180128047J

Jurišić T, Milić M, Todorović VS, Živković M, Jurišić M, Milić-Lemić A, Tihaček-Šojić L, Brković B. Peri-implant soft and hard tissue condition after alveolar ridge preservation with beta-tricalcium phosphate/type I collagen in the maxillary esthetic zone: A 1-year follow-up study. in Vojnosanitetski pregled. 2020;77(1):22-28.
doi:10.2298/VSP180128047J .

Jurišić, Tamara, Milić, Marija, Todorović, Vladimir S., Živković, Marko, Jurišić, Milan, Milić-Lemić, Aleksandra, Tihaček-Šojić, Ljiljana, Brković, Božidar, "Peri-implant soft and hard tissue condition after alveolar ridge preservation with beta-tricalcium phosphate/type I collagen in the maxillary esthetic zone: A 1-year follow-up study" in Vojnosanitetski pregled, 77, no. 1 (2020):22-28,
https://doi.org/10.2298/VSP180128047J . .

TY  - JOUR
AU  - Vučetić, Milan
AU  - Roganović, Jelena
AU  - Freilich, M.
AU  - Shafer, D.
AU  - Milić, Marija
AU  - Đukić, Ljiljana
AU  - Petrović, Nina
AU  - Marković, Evgenija
AU  - Marković, Aleksa
AU  - Brković, Božidar
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2481
AB  - Objective: To evaluate an association between bone levels of inflammation/oxidative stress mediators and postoperative discomfort after third molar conventional or piezosurgery. Material and methods: Twenty-six subjects with bilaterally impacted mandibular third molars, who underwent either piezo or conventional surgery, were included in a split-mouth design study. MicroRNA-21 (miR-21) expression, interleukin-1 beta (IL-1β), and vascular endothelial growth factor (VEGF) proteins, as well as superoxide dismutase (SOD) activity in alveolar bone, were evaluated. Pain intensity, the first pain appearance, analgesic first use and total dose taken, trismus, and swelling were clinically recorded. Results: MiR-21 expression was higher while VEGF protein was lower in piezosurgery vs. conventional groups. The differences in IL-1β protein and SOD activity were not significant between groups. The pain intensity on the first day was significantly decreased in piezosurgery group. The first pain appearance and the first analgesic taken were reported sooner in conventional vs. piezosurgical group. Significantly pronounced trismus on the third day following conventional surgery was found. In conventional group, significantly increased trismus was observed on the third compared to the first postoperative day. MiR-21 showed significant correlation with the first pain appearance. Conclusion: Delayed onset of less pronounced postoperative pain after piezosurgical vs. conventional extraction of impacted lower third molar was significantly associated with expression of bone miR-21. Clinical relevance: Alveolar bone miR-21 may reflect surgical stress and is associated with third molar postoperative pain onset.
PB  - Springer
T2  - Clinical Oral Investigations
T1  - Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort
DO  - 10.1007/s00784-020-03366-6
ER  - 

@article{
author = "Vučetić, Milan and Roganović, Jelena and Freilich, M. and Shafer, D. and Milić, Marija and Đukić, Ljiljana and Petrović, Nina and Marković, Evgenija and Marković, Aleksa and Brković, Božidar",
year = "2020",
abstract = "Objective: To evaluate an association between bone levels of inflammation/oxidative stress mediators and postoperative discomfort after third molar conventional or piezosurgery. Material and methods: Twenty-six subjects with bilaterally impacted mandibular third molars, who underwent either piezo or conventional surgery, were included in a split-mouth design study. MicroRNA-21 (miR-21) expression, interleukin-1 beta (IL-1β), and vascular endothelial growth factor (VEGF) proteins, as well as superoxide dismutase (SOD) activity in alveolar bone, were evaluated. Pain intensity, the first pain appearance, analgesic first use and total dose taken, trismus, and swelling were clinically recorded. Results: MiR-21 expression was higher while VEGF protein was lower in piezosurgery vs. conventional groups. The differences in IL-1β protein and SOD activity were not significant between groups. The pain intensity on the first day was significantly decreased in piezosurgery group. The first pain appearance and the first analgesic taken were reported sooner in conventional vs. piezosurgical group. Significantly pronounced trismus on the third day following conventional surgery was found. In conventional group, significantly increased trismus was observed on the third compared to the first postoperative day. MiR-21 showed significant correlation with the first pain appearance. Conclusion: Delayed onset of less pronounced postoperative pain after piezosurgical vs. conventional extraction of impacted lower third molar was significantly associated with expression of bone miR-21. Clinical relevance: Alveolar bone miR-21 may reflect surgical stress and is associated with third molar postoperative pain onset.",
publisher = "Springer",
journal = "Clinical Oral Investigations",
title = "Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort",
doi = "10.1007/s00784-020-03366-6"
}

Vučetić, M., Roganović, J., Freilich, M., Shafer, D., Milić, M., Đukić, L., Petrović, N., Marković, E., Marković, A.,& Brković, B.. (2020). Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort. in Clinical Oral Investigations
Springer..
https://doi.org/10.1007/s00784-020-03366-6

Vučetić M, Roganović J, Freilich M, Shafer D, Milić M, Đukić L, Petrović N, Marković E, Marković A, Brković B. Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort. in Clinical Oral Investigations. 2020;.
doi:10.1007/s00784-020-03366-6 .

Vučetić, Milan, Roganović, Jelena, Freilich, M., Shafer, D., Milić, Marija, Đukić, Ljiljana, Petrović, Nina, Marković, Evgenija, Marković, Aleksa, Brković, Božidar, "Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort" in Clinical Oral Investigations (2020),
https://doi.org/10.1007/s00784-020-03366-6 . .

TY  - JOUR
AU  - Ćetković, Dejan
AU  - Milić, Marija
AU  - Biočanin, Vladimir
AU  - Brajić, Ivana
AU  - Ćalasan, Dejan
AU  - Radović, Katarina
AU  - Živković, Rade
AU  - Brković, Božidar
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2448
AB  - Bacground/Aim. The efficient dental anesthesia, which is related to the clinically adequate depth, duration and the width of anesthetic field, is an important prerequisite for successful dental treatment. The aim of this study was to investigate and compare the pulpal anesthesia and cardiovascular parameters after the anterior middle superior alveolar (AMSA) nerve block with 4% articaine with epinephrine administered by conventional cartridge-syringe and computer-controlled local anesthetic delivery system (CCLADS). Methods. This controlled double-blind cross-over randomized clinical study included 38 healthy volunteers. Efficacy of pulpal anesthesia after the AMSA nerve block was evaluated by measuring a success rate, onset and duration of pulpal anesthesia, using an electrical pulp tester. The parameters of cardiovascular function (systolic and diastolic blood pressure, heart rate) were monitored noninvasively. Results. Successful pulpal anesthesia of all tested teeth was obtained in 57.9% participants with CCLADS and in 44.7% participants with conventional syringe. The onset time was not significantly different between two investigated groups. The pulpal anesthesia duration was not significantly different neither within nor between investigated groups. The systolic and diastolic blood pressures were significantly decreased in both investigated groups, in comparison with the baseline values. Heart rate significantly decreased within CCLADS from 10th to 30th minute when compared to baseline. Conclusion. The efficacy of pulpal anesthesia and safety of cardiovascular profile of 0.6 mL of articaine with epinephrine (1:100.000) delivered with CCLADS were improved in comparison to the conventional syringe delivery. Significant changes of cardiovascular function were not observed.
AB  - Uvod/Cilj. Efikasna zubna anestezija u pogledu klinički adekvatne dubine, trajanja i širine anestetičkog polja je važan preduslov za uspešno zubno lečenje. Cilj rada bio je ispitivanje i upoređivanje parametra anestezije zubne pulpe i kardiovaskularnih parametra posle gornje prednje i srednje alveolarne (AMSA) sprovodne anestezije postignute 4% artikainom sa epinefrinom, primenom standardne karpulbrizgalice i kompjuterski kontrolisanog sistema za primenu anestetičkog rastvora (CCLADS). Metode. U ovom randomizovanom, prospektivnom, kontrolisanom, dvostruko slepom ukrštenom kliničkom istraživanju učestvovalo je 38 ispitanika. Kvalitet anestezije zubne pulpe posle AMSA anestezije praćen je na osnovu uspešnosti, latentnog perioda i trajanja anestezije zubne pulpe, primenom električnog pulp-testera. Parametri kardiovaskularne funkcije (sistolni i dijastolni krvni pritisak, srčana frekvencija) praćeni su neinvazivno, primenom aparata za monitoring. Rezultati. Uspešna anestezija zubne pulpe svih ispitivanih zuba bila je prisutna kod 57,9% ispitanika posle kod CCLADS i kod 44,7% ispitanika posle primene standardne karpul-brizgalice. Latentni period i trajanje anestezije zubne pulpe nisu se značajno razlikovali između ispitivanih grupa. Sistolni i dijastolni pritisak bili su značajno sniženi u praćenim vremenskim intervalima u odnosu na početne vrednosti. Srčana frekvencija je bila značajno snižena kod CCLADS grupe od 10 do 30 minuta u odnosu na početne vrednosti. Zaključak. Kontrolisanom kompjuterizovanom primenom (CCLADS) 0,6 mL 4% artikaina sa epinefrinom (1:100,000) za AMSA sprovodnu anesteziju, postignut je bolji kvalitet anestezije zubne pulpe u odnosu na primenu artikaina sa epinefrinom standardnom karpul brizgalicom. Nisu uočene bitne promene funkcija kardiovaskularnog sistema.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Efficacy and safety of 4% articaine with epinephrine for the anterior middle superior alveolar nerve block comparing to the computer controlled and conventional anesthetic delivery: Prospective, randomized, cross-over clinical study
T1  - Efikasnost i sigurnost 4% artikaina sa epinefrinom za gornji prednji i srednji alveolarni nervni blok primenom kompjuterski kontrolisanog sistema i standardnog pristupa za primenu anestetičkog rastvora - prospektivna, randomizovana, kontrolisana, dvosturko slepa, ukrštena klinička studija
VL  - 76
IS  - 10
SP  - 1045
EP  - 1053
DO  - 10.2298/VSP170511013C
ER  - 

@article{
author = "Ćetković, Dejan and Milić, Marija and Biočanin, Vladimir and Brajić, Ivana and Ćalasan, Dejan and Radović, Katarina and Živković, Rade and Brković, Božidar",
year = "2019",
abstract = "Bacground/Aim. The efficient dental anesthesia, which is related to the clinically adequate depth, duration and the width of anesthetic field, is an important prerequisite for successful dental treatment. The aim of this study was to investigate and compare the pulpal anesthesia and cardiovascular parameters after the anterior middle superior alveolar (AMSA) nerve block with 4% articaine with epinephrine administered by conventional cartridge-syringe and computer-controlled local anesthetic delivery system (CCLADS). Methods. This controlled double-blind cross-over randomized clinical study included 38 healthy volunteers. Efficacy of pulpal anesthesia after the AMSA nerve block was evaluated by measuring a success rate, onset and duration of pulpal anesthesia, using an electrical pulp tester. The parameters of cardiovascular function (systolic and diastolic blood pressure, heart rate) were monitored noninvasively. Results. Successful pulpal anesthesia of all tested teeth was obtained in 57.9% participants with CCLADS and in 44.7% participants with conventional syringe. The onset time was not significantly different between two investigated groups. The pulpal anesthesia duration was not significantly different neither within nor between investigated groups. The systolic and diastolic blood pressures were significantly decreased in both investigated groups, in comparison with the baseline values. Heart rate significantly decreased within CCLADS from 10th to 30th minute when compared to baseline. Conclusion. The efficacy of pulpal anesthesia and safety of cardiovascular profile of 0.6 mL of articaine with epinephrine (1:100.000) delivered with CCLADS were improved in comparison to the conventional syringe delivery. Significant changes of cardiovascular function were not observed., Uvod/Cilj. Efikasna zubna anestezija u pogledu klinički adekvatne dubine, trajanja i širine anestetičkog polja je važan preduslov za uspešno zubno lečenje. Cilj rada bio je ispitivanje i upoređivanje parametra anestezije zubne pulpe i kardiovaskularnih parametra posle gornje prednje i srednje alveolarne (AMSA) sprovodne anestezije postignute 4% artikainom sa epinefrinom, primenom standardne karpulbrizgalice i kompjuterski kontrolisanog sistema za primenu anestetičkog rastvora (CCLADS). Metode. U ovom randomizovanom, prospektivnom, kontrolisanom, dvostruko slepom ukrštenom kliničkom istraživanju učestvovalo je 38 ispitanika. Kvalitet anestezije zubne pulpe posle AMSA anestezije praćen je na osnovu uspešnosti, latentnog perioda i trajanja anestezije zubne pulpe, primenom električnog pulp-testera. Parametri kardiovaskularne funkcije (sistolni i dijastolni krvni pritisak, srčana frekvencija) praćeni su neinvazivno, primenom aparata za monitoring. Rezultati. Uspešna anestezija zubne pulpe svih ispitivanih zuba bila je prisutna kod 57,9% ispitanika posle kod CCLADS i kod 44,7% ispitanika posle primene standardne karpul-brizgalice. Latentni period i trajanje anestezije zubne pulpe nisu se značajno razlikovali između ispitivanih grupa. Sistolni i dijastolni pritisak bili su značajno sniženi u praćenim vremenskim intervalima u odnosu na početne vrednosti. Srčana frekvencija je bila značajno snižena kod CCLADS grupe od 10 do 30 minuta u odnosu na početne vrednosti. Zaključak. Kontrolisanom kompjuterizovanom primenom (CCLADS) 0,6 mL 4% artikaina sa epinefrinom (1:100,000) za AMSA sprovodnu anesteziju, postignut je bolji kvalitet anestezije zubne pulpe u odnosu na primenu artikaina sa epinefrinom standardnom karpul brizgalicom. Nisu uočene bitne promene funkcija kardiovaskularnog sistema.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Efficacy and safety of 4% articaine with epinephrine for the anterior middle superior alveolar nerve block comparing to the computer controlled and conventional anesthetic delivery: Prospective, randomized, cross-over clinical study, Efikasnost i sigurnost 4% artikaina sa epinefrinom za gornji prednji i srednji alveolarni nervni blok primenom kompjuterski kontrolisanog sistema i standardnog pristupa za primenu anestetičkog rastvora - prospektivna, randomizovana, kontrolisana, dvosturko slepa, ukrštena klinička studija",
volume = "76",
number = "10",
pages = "1045-1053",
doi = "10.2298/VSP170511013C"
}

Ćetković, D., Milić, M., Biočanin, V., Brajić, I., Ćalasan, D., Radović, K., Živković, R.,& Brković, B.. (2019). Efficacy and safety of 4% articaine with epinephrine for the anterior middle superior alveolar nerve block comparing to the computer controlled and conventional anesthetic delivery: Prospective, randomized, cross-over clinical study. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 76(10), 1045-1053.
https://doi.org/10.2298/VSP170511013C

Ćetković D, Milić M, Biočanin V, Brajić I, Ćalasan D, Radović K, Živković R, Brković B. Efficacy and safety of 4% articaine with epinephrine for the anterior middle superior alveolar nerve block comparing to the computer controlled and conventional anesthetic delivery: Prospective, randomized, cross-over clinical study. in Vojnosanitetski pregled. 2019;76(10):1045-1053.
doi:10.2298/VSP170511013C .

Ćetković, Dejan, Milić, Marija, Biočanin, Vladimir, Brajić, Ivana, Ćalasan, Dejan, Radović, Katarina, Živković, Rade, Brković, Božidar, "Efficacy and safety of 4% articaine with epinephrine for the anterior middle superior alveolar nerve block comparing to the computer controlled and conventional anesthetic delivery: Prospective, randomized, cross-over clinical study" in Vojnosanitetski pregled, 76, no. 10 (2019):1045-1053,
https://doi.org/10.2298/VSP170511013C . .

TY  - JOUR
AU  - Kopčalić, Katarina
AU  - Petrović, Nina
AU  - Stanojković, Tatjana P.
AU  - Stanković, Vesna
AU  - Bukumirić, Zoran
AU  - Roganović, Jelena
AU  - Mališić, Emina
AU  - Nikitović, Marina
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2594
AB  - Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. 
Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative ΔΔCt method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. 
Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). 
Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs.
PB  - Elsevier GmbH
T2  - Pathology - Research and Practice
T1  - Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study
VL  - 215
IS  - 4
SP  - 626
EP  - 631
DO  - 10.1016/j.prp.2018.12.007
ER  - 

@article{
author = "Kopčalić, Katarina and Petrović, Nina and Stanojković, Tatjana P. and Stanković, Vesna and Bukumirić, Zoran and Roganović, Jelena and Mališić, Emina and Nikitović, Marina",
year = "2019",
abstract = "Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. 
Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative ΔΔCt method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. 
Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). 
Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs.",
publisher = "Elsevier GmbH",
journal = "Pathology - Research and Practice",
title = "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study",
volume = "215",
number = "4",
pages = "626-631",
doi = "10.1016/j.prp.2018.12.007"
}

Kopčalić, K., Petrović, N., Stanojković, T. P., Stanković, V., Bukumirić, Z., Roganović, J., Mališić, E.,& Nikitović, M.. (2019). Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology - Research and Practice
Elsevier GmbH., 215(4), 626-631.
https://doi.org/10.1016/j.prp.2018.12.007

Kopčalić K, Petrović N, Stanojković TP, Stanković V, Bukumirić Z, Roganović J, Mališić E, Nikitović M. Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology - Research and Practice. 2019;215(4):626-631.
doi:10.1016/j.prp.2018.12.007 .

Kopčalić, Katarina, Petrović, Nina, Stanojković, Tatjana P., Stanković, Vesna, Bukumirić, Zoran, Roganović, Jelena, Mališić, Emina, Nikitović, Marina, "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study" in Pathology - Research and Practice, 215, no. 4 (2019):626-631,
https://doi.org/10.1016/j.prp.2018.12.007 . .

TY  - THES
AU  - Radović, Nikola
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7320
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:21240/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51881743
UR  - http://nardus.mpn.gov.rs/123456789/12161
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1045
AB  - Recent studies point at the crucial role of genetic and epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF), as well as levels of MMP-9, in periodontitis patients with (CPDM) and without DM type 2 (CP), as well as in periodontally healthy, control groups with or without DM type 2 (PHDM and PH, respectively). Ninety-six subjects (44 males and 52 females) were included in the study. Using a periodontal probe (PCPUNC 15, Hu Friedy, Chicago, USA), full-mouth periodontal evaluation was carried out. The following clinical parameters were recorded: probing pocket depth (PPD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI), and plaque index (PI). Baseline, GCF samples were collected from two representative sites per participant, 24h after the clinical measurments were recorded using paper strip, PerioPaper®, (Pro-Flow Corp., Amityville, NY, USA). Representative sites in periodontally healthy participants were mesiovestibular aspect of the right upper central incisor and right upper first molar. In patients with periodontitis, the two sites demonstrating the highest values of PPD and positive BOP were considered as representative. The clinical measurements were recorded, and GCF samples were taken from the sampling site at baseline and 6 weeks after the periodontal treatment. MiRNA modulation was analyzed using real-time quantitative reverse transcriptase- polymerase chain reaction qRT-PCR. SOD activity was measured spectrophotometrically, while the levels of MMP-9 was measured using enzyme-linked immunosorbent assay (ELISA). Mean values of full-mouth and sample sites’ PPD, CAL, BOP, GI, PI were statistically higher in CP and CPDM groups compared to periodontally healthy groups (PH and PHDM), baseline as well as 6 weeks after non-surgical therapy. (Anova and Bonferroni post hoc test. p lt 0.01). The upregulation of miR-146a and miR-155 was observed baseline in CP and CPDM patients, while the levels decreased six weeks after the non-surgical therapy to the levels comparable to PH and PHDM, respectively. (Mann-Whitney test, p lt 0.01)...
AB  - Nedavne studije pokazuju ključnu ulogu genetičkih i epigenetičkih mehanizama u razvoju multifaktorijalnih oboljenja kao što su parodontopatija i dijabetes melitus tip 2 (DM tip 2). Tako su se cirkulatorne miRNK pojavile kao potencijalni novi biomarkeri mnogih oboljenja i igraju važnu ulogu u regulaciji značajnih bioloških procesa među kojima je i inflamacija. Cilj ove studije je bio da se istraže vrednosti i značaj nivoa ekspresije molekula miRNK-146a i miRNK-155, nivo aktivnosti enzima SOD-e (superoksid dizmutaze), kao i vrednosti koncentracije enzima matriksne metaloproteinaze 9 (MMP-9) u gingivalnoj tečnosti ispitanika obolelih od hronične parodontopatije sistemski zdravih (HP grupa) i obolelih od DM tipa 2 (HPDM grupa) i ispitanika sa klinički zdravim parodoncijumom sistemski zdravih (Z grupa) i obolelih od DM tipa 2 (ZDM grupa). U istraživanje je bilo uključeno 96 ispitanika oba pola (44 muškaraca i 52 žene). Nivo oralne higijene i stanje parodontalnih tkiva verifikovano je pomoću graduisane parodontalne sonde (PCPUNC 15, Hu Friedy, Chicago, USA) merenjem sledećih kliničkih parametara: plak indeks, gingivalni indeks, krvarenje na provokaciju, dubina sondiranja, nivo pripojnog epitela i nivo ivice gingive. Uzorci gingivalne tečnosti su uzimani sa dva reprezentativna mesta filter papirima PerioPaper®, (Pro-Flow Corp., Amityville, NY, USA), 24h nakon merenja i beleženja kliničkih parametara. Ispitanicima sa hroničnom parodontopatijom uzorci su uzimani iz dva parodontalna džepa, na mestima sa najvećim vrednostima dubine sondiranja i pozitivnim vrednostima krvarenja na provokaciju, dok je ispitanicima sa zdravim parodoncijumom uzorak gingivalne tečnosti uziman u regiji gornjeg desnog centralnog sekutića i gornjeg desnog prvog molara (meziovestibularne površine). Klinička merenja i uzorkovanje gingivalne tečnosti su rađena dva puta: na prvom pregledu i šest nedelja nakon sprovedene kauzalne terapije obolelog parodoncijuma. Vrednosti nivoa ekspresije molekula miRNK su određivane lančanom reakcijom polimeraze kojoj prethodi reverzna transkripcija korišćenjem prajmera sa petljom, (eng. stem-loop) qRT-PCR (eng. real-time quantitative reverse transcriptase- polymerase chain reaction). Aktivnost enzima SOD-e je određivana spektrofotometrijskom metodom, dok su vrednosti koncentracije molekula MMP-9 mereni ELISA metodom (eng. enzyme-linked immunosorbent assay)...
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in nondiabetic and type 2 diabetic patients
T1  - Mikro RNK-146a i mikro RNK-155 u gingivalnoj tečnosti, kao potencijalni biomarkeri parodontopatije kod sistemski zdravih osoba i obolelih od dijabetes melitusa tip 2
UR  - https://hdl.handle.net/21.15107/rcub_nardus_12161
ER  - 

@phdthesis{
author = "Radović, Nikola",
year = "2019",
abstract = "Recent studies point at the crucial role of genetic and epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF), as well as levels of MMP-9, in periodontitis patients with (CPDM) and without DM type 2 (CP), as well as in periodontally healthy, control groups with or without DM type 2 (PHDM and PH, respectively). Ninety-six subjects (44 males and 52 females) were included in the study. Using a periodontal probe (PCPUNC 15, Hu Friedy, Chicago, USA), full-mouth periodontal evaluation was carried out. The following clinical parameters were recorded: probing pocket depth (PPD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI), and plaque index (PI). Baseline, GCF samples were collected from two representative sites per participant, 24h after the clinical measurments were recorded using paper strip, PerioPaper®, (Pro-Flow Corp., Amityville, NY, USA). Representative sites in periodontally healthy participants were mesiovestibular aspect of the right upper central incisor and right upper first molar. In patients with periodontitis, the two sites demonstrating the highest values of PPD and positive BOP were considered as representative. The clinical measurements were recorded, and GCF samples were taken from the sampling site at baseline and 6 weeks after the periodontal treatment. MiRNA modulation was analyzed using real-time quantitative reverse transcriptase- polymerase chain reaction qRT-PCR. SOD activity was measured spectrophotometrically, while the levels of MMP-9 was measured using enzyme-linked immunosorbent assay (ELISA). Mean values of full-mouth and sample sites’ PPD, CAL, BOP, GI, PI were statistically higher in CP and CPDM groups compared to periodontally healthy groups (PH and PHDM), baseline as well as 6 weeks after non-surgical therapy. (Anova and Bonferroni post hoc test. p lt 0.01). The upregulation of miR-146a and miR-155 was observed baseline in CP and CPDM patients, while the levels decreased six weeks after the non-surgical therapy to the levels comparable to PH and PHDM, respectively. (Mann-Whitney test, p lt 0.01)..., Nedavne studije pokazuju ključnu ulogu genetičkih i epigenetičkih mehanizama u razvoju multifaktorijalnih oboljenja kao što su parodontopatija i dijabetes melitus tip 2 (DM tip 2). Tako su se cirkulatorne miRNK pojavile kao potencijalni novi biomarkeri mnogih oboljenja i igraju važnu ulogu u regulaciji značajnih bioloških procesa među kojima je i inflamacija. Cilj ove studije je bio da se istraže vrednosti i značaj nivoa ekspresije molekula miRNK-146a i miRNK-155, nivo aktivnosti enzima SOD-e (superoksid dizmutaze), kao i vrednosti koncentracije enzima matriksne metaloproteinaze 9 (MMP-9) u gingivalnoj tečnosti ispitanika obolelih od hronične parodontopatije sistemski zdravih (HP grupa) i obolelih od DM tipa 2 (HPDM grupa) i ispitanika sa klinički zdravim parodoncijumom sistemski zdravih (Z grupa) i obolelih od DM tipa 2 (ZDM grupa). U istraživanje je bilo uključeno 96 ispitanika oba pola (44 muškaraca i 52 žene). Nivo oralne higijene i stanje parodontalnih tkiva verifikovano je pomoću graduisane parodontalne sonde (PCPUNC 15, Hu Friedy, Chicago, USA) merenjem sledećih kliničkih parametara: plak indeks, gingivalni indeks, krvarenje na provokaciju, dubina sondiranja, nivo pripojnog epitela i nivo ivice gingive. Uzorci gingivalne tečnosti su uzimani sa dva reprezentativna mesta filter papirima PerioPaper®, (Pro-Flow Corp., Amityville, NY, USA), 24h nakon merenja i beleženja kliničkih parametara. Ispitanicima sa hroničnom parodontopatijom uzorci su uzimani iz dva parodontalna džepa, na mestima sa najvećim vrednostima dubine sondiranja i pozitivnim vrednostima krvarenja na provokaciju, dok je ispitanicima sa zdravim parodoncijumom uzorak gingivalne tečnosti uziman u regiji gornjeg desnog centralnog sekutića i gornjeg desnog prvog molara (meziovestibularne površine). Klinička merenja i uzorkovanje gingivalne tečnosti su rađena dva puta: na prvom pregledu i šest nedelja nakon sprovedene kauzalne terapije obolelog parodoncijuma. Vrednosti nivoa ekspresije molekula miRNK su određivane lančanom reakcijom polimeraze kojoj prethodi reverzna transkripcija korišćenjem prajmera sa petljom, (eng. stem-loop) qRT-PCR (eng. real-time quantitative reverse transcriptase- polymerase chain reaction). Aktivnost enzima SOD-e je određivana spektrofotometrijskom metodom, dok su vrednosti koncentracije molekula MMP-9 mereni ELISA metodom (eng. enzyme-linked immunosorbent assay)...",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in nondiabetic and type 2 diabetic patients, Mikro RNK-146a i mikro RNK-155 u gingivalnoj tečnosti, kao potencijalni biomarkeri parodontopatije kod sistemski zdravih osoba i obolelih od dijabetes melitusa tip 2",
url = "https://hdl.handle.net/21.15107/rcub_nardus_12161"
}

Radović, N.. (2019). MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in nondiabetic and type 2 diabetic patients. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_12161

Radović N. MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in nondiabetic and type 2 diabetic patients. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_12161 .

Radović, Nikola, "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in nondiabetic and type 2 diabetic patients" (2019),
https://hdl.handle.net/21.15107/rcub_nardus_12161 .

TY  - THES
AU  - Vasović, Dina
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7283
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:21040/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51891471
UR  - http://nardus.mpn.gov.rs/123456789/12098
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1043
AB  - poorly controlled by current treatments. It represents major therapeutic concern and reduces patients quality of life. Numerous chemical mediators are present in trigeminal ganglia, and GABA is most common inhbitory neurotransmitter. Due to different subunit combinations, GABA can show diversity of pharmacological effects. GABAA receptors containing α6 subunit are located in trigeminal ganglia, and their reduction by small interfering RNA increases inflammatory temporomandibular and myofascial pain in rats. One of the most common orofacial pain states like trigeminal neuralgia or trigeminal neuropathy begin in trigeminal ganglia. We thus hypothesized that enhancing GABAA receptors containing the α6 subunit activity may help in neuropathic syndromes originating from the trigeminal system. Selective modulators that activate this specific receptor population are not commercially available. In this doctoral dissertation we used recently developed deuterated pyrazoloquinolinone compound, (DK-I-56-1) that selectively activates α6 subunit of GABAA receptors. Here, we performed a detailed pharmacokinetic analysis of DK-I-56-1 on animal experimental model. Both plasma and brain tissue kinetics of DK-I-56-1 were relatively slow, with half-lives of 6 h and 13 h, respectively, enabling the persistence of estimated free brain concentrations in the range 10-300 nM throughout a 24-h period. We confirmed that chronic constriction injury of infraorbital nerve is considered a reliable experimental animal model for trigeminal neuropathic pain. Results were obtained on IoN-CCI protocol in hypersensitive rats dosed intraperitoneally with 10 mg/kg DK-I-56-1 or DK-I-87-1, structurally similar pyrazoloquinolinone compound that can not induce GABA currents on α6 containing receptors...
AB  - Trigeminalni neuropatski bol je hronično bolno stanje orofacijalne regije koje nastaje kao posledica povrede trigeminalnog živca. Ova vrsta bola loše ili uopšte ne reaguje na terapiju, te njegovo lečenje predstavlja veliki terapijski problem koji utiče kvalitet života pacijenta. U trigeminalnom ganglionu eksprimiran je veliki broj hemijskih medijatora, a GABA je najznačajniji inhibitorni neurotransmiter. Zbog velikog broja podjedinica i njihovih različitih kombinacija, postoji veliki broj efekata koji GABA ostvaruje. U trigeminalnom ganglionu postoje GABAA receptori koji sadrže α6 podjedinicu. Smanjenje ove podjedinice primenom male interferirajuće RNK pojačava bolni odgovor kod eksperimentalnh životinja na modelima miofacijalnog i inflamatornog bola temporomandibularnog zgloba. Trigeminalni ganglion je supstrat za razvoj većeg broja patoloških stanja koje se sreću u stomatologiji, kao što su trigeminalna neuralgija i trigerminalna neuropatija. Hipoteza ove doktorske disertacije je bila da pojačavanje aktivnosti GABAA receptora koji sadrže α6 podjedinicu utiče na smanjenje bolnog odgovora u modelu trigeminalnog neuropatskog bola. Lekovi koji potenciraju aktivnost ove grupe receptora nisu dostupni u kliničkoj praksi. U ovoj doktorskoj disertaciji korišćen je DK-I-56-1, novosintetisani selektivni modulator α6 podjedinice GABAA receptora, koji pripada grupi deuterisanih pirazolohinolinona. Sprovedena je detaljna farmakokinetička analiza ovog jedinjenja na animalnom eksperimentalnom modelu. Dobijeni rezultati pokazuju da je kinetika ovog jedinjenja u plazmi i mozgu relativno spora, sa poluvremenom eliminacije od 6 h odnosno 13 h, pri čemu je postignuta slobodna koncentracija u mozgu u rasponu 10-300 nM. Ovakav farmakokinetički profil je podesan za ispitivanje u protokolima ponavljane primene...
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - Selective modulation of alpha6 subunit containing GABAA receptors on trigeminal neuropathic pain model
T1  - Selektivna modulacija GABAA receptora koji sadrže alfa6 podjedinicu na eksperimentalnom modelu trigeminalnog neuropatskog bola
UR  - https://hdl.handle.net/21.15107/rcub_nardus_12098
ER  - 

@phdthesis{
author = "Vasović, Dina",
year = "2019",
abstract = "poorly controlled by current treatments. It represents major therapeutic concern and reduces patients quality of life. Numerous chemical mediators are present in trigeminal ganglia, and GABA is most common inhbitory neurotransmitter. Due to different subunit combinations, GABA can show diversity of pharmacological effects. GABAA receptors containing α6 subunit are located in trigeminal ganglia, and their reduction by small interfering RNA increases inflammatory temporomandibular and myofascial pain in rats. One of the most common orofacial pain states like trigeminal neuralgia or trigeminal neuropathy begin in trigeminal ganglia. We thus hypothesized that enhancing GABAA receptors containing the α6 subunit activity may help in neuropathic syndromes originating from the trigeminal system. Selective modulators that activate this specific receptor population are not commercially available. In this doctoral dissertation we used recently developed deuterated pyrazoloquinolinone compound, (DK-I-56-1) that selectively activates α6 subunit of GABAA receptors. Here, we performed a detailed pharmacokinetic analysis of DK-I-56-1 on animal experimental model. Both plasma and brain tissue kinetics of DK-I-56-1 were relatively slow, with half-lives of 6 h and 13 h, respectively, enabling the persistence of estimated free brain concentrations in the range 10-300 nM throughout a 24-h period. We confirmed that chronic constriction injury of infraorbital nerve is considered a reliable experimental animal model for trigeminal neuropathic pain. Results were obtained on IoN-CCI protocol in hypersensitive rats dosed intraperitoneally with 10 mg/kg DK-I-56-1 or DK-I-87-1, structurally similar pyrazoloquinolinone compound that can not induce GABA currents on α6 containing receptors..., Trigeminalni neuropatski bol je hronično bolno stanje orofacijalne regije koje nastaje kao posledica povrede trigeminalnog živca. Ova vrsta bola loše ili uopšte ne reaguje na terapiju, te njegovo lečenje predstavlja veliki terapijski problem koji utiče kvalitet života pacijenta. U trigeminalnom ganglionu eksprimiran je veliki broj hemijskih medijatora, a GABA je najznačajniji inhibitorni neurotransmiter. Zbog velikog broja podjedinica i njihovih različitih kombinacija, postoji veliki broj efekata koji GABA ostvaruje. U trigeminalnom ganglionu postoje GABAA receptori koji sadrže α6 podjedinicu. Smanjenje ove podjedinice primenom male interferirajuće RNK pojačava bolni odgovor kod eksperimentalnh životinja na modelima miofacijalnog i inflamatornog bola temporomandibularnog zgloba. Trigeminalni ganglion je supstrat za razvoj većeg broja patoloških stanja koje se sreću u stomatologiji, kao što su trigeminalna neuralgija i trigerminalna neuropatija. Hipoteza ove doktorske disertacije je bila da pojačavanje aktivnosti GABAA receptora koji sadrže α6 podjedinicu utiče na smanjenje bolnog odgovora u modelu trigeminalnog neuropatskog bola. Lekovi koji potenciraju aktivnost ove grupe receptora nisu dostupni u kliničkoj praksi. U ovoj doktorskoj disertaciji korišćen je DK-I-56-1, novosintetisani selektivni modulator α6 podjedinice GABAA receptora, koji pripada grupi deuterisanih pirazolohinolinona. Sprovedena je detaljna farmakokinetička analiza ovog jedinjenja na animalnom eksperimentalnom modelu. Dobijeni rezultati pokazuju da je kinetika ovog jedinjenja u plazmi i mozgu relativno spora, sa poluvremenom eliminacije od 6 h odnosno 13 h, pri čemu je postignuta slobodna koncentracija u mozgu u rasponu 10-300 nM. Ovakav farmakokinetički profil je podesan za ispitivanje u protokolima ponavljane primene...",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "Selective modulation of alpha6 subunit containing GABAA receptors on trigeminal neuropathic pain model, Selektivna modulacija GABAA receptora koji sadrže alfa6 podjedinicu na eksperimentalnom modelu trigeminalnog neuropatskog bola",
url = "https://hdl.handle.net/21.15107/rcub_nardus_12098"
}

Vasović, D.. (2019). Selective modulation of alpha6 subunit containing GABAA receptors on trigeminal neuropathic pain model. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_12098

Vasović D. Selective modulation of alpha6 subunit containing GABAA receptors on trigeminal neuropathic pain model. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_12098 .

Vasović, Dina, "Selective modulation of alpha6 subunit containing GABAA receptors on trigeminal neuropathic pain model" (2019),
https://hdl.handle.net/21.15107/rcub_nardus_12098 .

TY  - JOUR
AU  - Kopcalić, Katarina
AU  - Petrović, Nina
AU  - Stanojković, Tatjana P.
AU  - Stanković, Vesna
AU  - Bukumirić, Zoran
AU  - Roganović, Jelena
AU  - Malisić, Emina
AU  - Nikitović, Marina
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2391
AB  - Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative Delta Delta Ct method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs.
PB  - Elsevier Gmbh, Munich
T2  - Pathology Research & Practice
T1  - Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study
VL  - 215
IS  - 4
SP  - 626
EP  - 631
DO  - 10.1016/j.prp.2018.12.007
ER  - 

@article{
author = "Kopcalić, Katarina and Petrović, Nina and Stanojković, Tatjana P. and Stanković, Vesna and Bukumirić, Zoran and Roganović, Jelena and Malisić, Emina and Nikitović, Marina",
year = "2019",
abstract = "Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative Delta Delta Ct method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs.",
publisher = "Elsevier Gmbh, Munich",
journal = "Pathology Research & Practice",
title = "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study",
volume = "215",
number = "4",
pages = "626-631",
doi = "10.1016/j.prp.2018.12.007"
}

Kopcalić, K., Petrović, N., Stanojković, T. P., Stanković, V., Bukumirić, Z., Roganović, J., Malisić, E.,& Nikitović, M.. (2019). Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology Research & Practice
Elsevier Gmbh, Munich., 215(4), 626-631.
https://doi.org/10.1016/j.prp.2018.12.007

Kopcalić K, Petrović N, Stanojković TP, Stanković V, Bukumirić Z, Roganović J, Malisić E, Nikitović M. Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology Research & Practice. 2019;215(4):626-631.
doi:10.1016/j.prp.2018.12.007 .

Kopcalić, Katarina, Petrović, Nina, Stanojković, Tatjana P., Stanković, Vesna, Bukumirić, Zoran, Roganović, Jelena, Malisić, Emina, Nikitović, Marina, "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study" in Pathology Research & Practice, 215, no. 4 (2019):626-631,
https://doi.org/10.1016/j.prp.2018.12.007 . .

TY  - JOUR
AU  - Baćević, Miljana
AU  - Brković, Božidar
AU  - Lamber, France
AU  - Đukić, Ljiljana
AU  - Petrović, Nina
AU  - Roganović, Jelena
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2388
AB  - Objective: Leukocyte- and platelet-rich fibrin (L-PRF) represents a natural, low-cost product which may promote tissue healing by mechanisms not fully elucidated. Diabetes mellitus (DM) disrupts bone healing by inducing inflammation and oxidative stress (OS), mechanisms regulated by microRNAs (miRs). The aim of the present study was to investigate the microRNA-21 (miR-21) involvement in diabetic bone regeneration using L-PRF alone or in combination with a standard grafting material. Design: After the induction of diabetes (alloxan 100 mg/kg), four cranial osteotomies were made in diabetic (n = 12) and non-diabetic (n = 12) rabbits: one was left empty and the remaining three were grafted with L-PRF, bovine hydroxyapatite (Bio-Oss((R))) and L-PRF + Bio-Oss((R)). Two and eight weeks postoperatively, the samples were harvested for miR-21 expression (Real-time RT-PCR) and enzyme-linked immunosorbent assay analyses. Results: Diabetic rabbits showed decreased miR-21 and matrix metalloproteinase-9 (MMP-9) protein expression while increased malondialdehyde (MDA) levels two weeks postoperatively; however, there were no significant differences in miR-21 and MMP-9 levels between diabetic and non-diabetic rabbits in samples taken eight weeks postoperatively. Application of L-PRF and L-PRF + Bio-Oss((R)) improved miR-21 and MMP-9 and decreased MDA levels while Bio-Oss((R)) alone enhanced superoxide dismutase (SOD) activity levels in diabetic rabbits. Conclusion: L-PRF alone or in combination with bovine hydroxyapatite as bone graft could be beneficial in DM since it seems to improve inflammation-modulatory miR-21 expression and decreases oxidative stress.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Archives of Oral Biology
T1  - Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits
VL  - 102
SP  - 231
EP  - 237
DO  - 10.1016/j.archoralbio.2019.05.005
ER  - 

@article{
author = "Baćević, Miljana and Brković, Božidar and Lamber, France and Đukić, Ljiljana and Petrović, Nina and Roganović, Jelena",
year = "2019",
abstract = "Objective: Leukocyte- and platelet-rich fibrin (L-PRF) represents a natural, low-cost product which may promote tissue healing by mechanisms not fully elucidated. Diabetes mellitus (DM) disrupts bone healing by inducing inflammation and oxidative stress (OS), mechanisms regulated by microRNAs (miRs). The aim of the present study was to investigate the microRNA-21 (miR-21) involvement in diabetic bone regeneration using L-PRF alone or in combination with a standard grafting material. Design: After the induction of diabetes (alloxan 100 mg/kg), four cranial osteotomies were made in diabetic (n = 12) and non-diabetic (n = 12) rabbits: one was left empty and the remaining three were grafted with L-PRF, bovine hydroxyapatite (Bio-Oss((R))) and L-PRF + Bio-Oss((R)). Two and eight weeks postoperatively, the samples were harvested for miR-21 expression (Real-time RT-PCR) and enzyme-linked immunosorbent assay analyses. Results: Diabetic rabbits showed decreased miR-21 and matrix metalloproteinase-9 (MMP-9) protein expression while increased malondialdehyde (MDA) levels two weeks postoperatively; however, there were no significant differences in miR-21 and MMP-9 levels between diabetic and non-diabetic rabbits in samples taken eight weeks postoperatively. Application of L-PRF and L-PRF + Bio-Oss((R)) improved miR-21 and MMP-9 and decreased MDA levels while Bio-Oss((R)) alone enhanced superoxide dismutase (SOD) activity levels in diabetic rabbits. Conclusion: L-PRF alone or in combination with bovine hydroxyapatite as bone graft could be beneficial in DM since it seems to improve inflammation-modulatory miR-21 expression and decreases oxidative stress.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Archives of Oral Biology",
title = "Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits",
volume = "102",
pages = "231-237",
doi = "10.1016/j.archoralbio.2019.05.005"
}

Baćević, M., Brković, B., Lamber, F., Đukić, L., Petrović, N.,& Roganović, J.. (2019). Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits. in Archives of Oral Biology
Pergamon-Elsevier Science Ltd, Oxford., 102, 231-237.
https://doi.org/10.1016/j.archoralbio.2019.05.005

Baćević M, Brković B, Lamber F, Đukić L, Petrović N, Roganović J. Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits. in Archives of Oral Biology. 2019;102:231-237.
doi:10.1016/j.archoralbio.2019.05.005 .

Baćević, Miljana, Brković, Božidar, Lamber, France, Đukić, Ljiljana, Petrović, Nina, Roganović, Jelena, "Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits" in Archives of Oral Biology, 102 (2019):231-237,
https://doi.org/10.1016/j.archoralbio.2019.05.005 . .

TY  - JOUR
AU  - Ćetković, Dejan
AU  - Antić, Svetlana
AU  - Antonijević, Đorđe
AU  - Brković, Božidar
AU  - Đukić, Ksenija
AU  - Vujašković, Goran
AU  - Đurić, Marija
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2318
AB  - Background. The authors investigated morphologic parameters of the palatal cortex that affect the diffusion of local anesthetic solution in the region of the anterior middle superior alveolar (AMSA) nerve block injection site. Methods. The authors used computed tomographic (CT) and micro-CT imaging to assess 20 human skulls from an anatomic collection. Analysis of the C1 images included frequency, distribution, and width of the nutrient canals in the bony palate, according to to the person's sex and age. Micro-CT analysis involved measuring the thickness and porosity of palatal cortical bone in the area of the AMSA injection site in relation to the thickness and porosity of the opposite buccal cortical bone. Results. There was a statistically significant difference (P = .042) in the location of the nutrient canals between male specimens (> 50% in the border region) and female specimens (> 50% in the palatal process). Furthermore, the female skulls had significantly wider nutrient canal foramina (P = .042) than did the male skulls. Despite greater thickness, the palatal cortex in the area of the AMSA injection site had slightly greater porosity than did the buccal cortex. A significantly greater number of microcanals penetrated the whole cortical thickness in palatal than in buccal cortical bone (P = .001). Conclusions. The distribution and width of nutrient canals differed between male and female skulls. At the microscopic level, structural characteristics of the palatal cortex provide a good anatomic basis for the potential of a satisfactory AMSA injection success rate. Practical Implications. The AMSA technique success rate might be increased if the clinician adjusts the injection site to the distribution of nutrient canals, depending on the sex of the patient.
PB  - Amer Dental Assoc, Chicago
T2  - Journal of the American Dental Association
T1  - Nutrient canals and porosity of the bony palate A basis for the biological plausibility of the anterior middle superior alveolar nerve block
VL  - 149
IS  - 10
SP  - 859
EP  - 868
DO  - 10.1016/j.adaj.2018.05.015
ER  - 

@article{
author = "Ćetković, Dejan and Antić, Svetlana and Antonijević, Đorđe and Brković, Božidar and Đukić, Ksenija and Vujašković, Goran and Đurić, Marija",
year = "2018",
abstract = "Background. The authors investigated morphologic parameters of the palatal cortex that affect the diffusion of local anesthetic solution in the region of the anterior middle superior alveolar (AMSA) nerve block injection site. Methods. The authors used computed tomographic (CT) and micro-CT imaging to assess 20 human skulls from an anatomic collection. Analysis of the C1 images included frequency, distribution, and width of the nutrient canals in the bony palate, according to to the person's sex and age. Micro-CT analysis involved measuring the thickness and porosity of palatal cortical bone in the area of the AMSA injection site in relation to the thickness and porosity of the opposite buccal cortical bone. Results. There was a statistically significant difference (P = .042) in the location of the nutrient canals between male specimens (> 50% in the border region) and female specimens (> 50% in the palatal process). Furthermore, the female skulls had significantly wider nutrient canal foramina (P = .042) than did the male skulls. Despite greater thickness, the palatal cortex in the area of the AMSA injection site had slightly greater porosity than did the buccal cortex. A significantly greater number of microcanals penetrated the whole cortical thickness in palatal than in buccal cortical bone (P = .001). Conclusions. The distribution and width of nutrient canals differed between male and female skulls. At the microscopic level, structural characteristics of the palatal cortex provide a good anatomic basis for the potential of a satisfactory AMSA injection success rate. Practical Implications. The AMSA technique success rate might be increased if the clinician adjusts the injection site to the distribution of nutrient canals, depending on the sex of the patient.",
publisher = "Amer Dental Assoc, Chicago",
journal = "Journal of the American Dental Association",
title = "Nutrient canals and porosity of the bony palate A basis for the biological plausibility of the anterior middle superior alveolar nerve block",
volume = "149",
number = "10",
pages = "859-868",
doi = "10.1016/j.adaj.2018.05.015"
}

Ćetković, D., Antić, S., Antonijević, Đ., Brković, B., Đukić, K., Vujašković, G.,& Đurić, M.. (2018). Nutrient canals and porosity of the bony palate A basis for the biological plausibility of the anterior middle superior alveolar nerve block. in Journal of the American Dental Association
Amer Dental Assoc, Chicago., 149(10), 859-868.
https://doi.org/10.1016/j.adaj.2018.05.015

Ćetković D, Antić S, Antonijević Đ, Brković B, Đukić K, Vujašković G, Đurić M. Nutrient canals and porosity of the bony palate A basis for the biological plausibility of the anterior middle superior alveolar nerve block. in Journal of the American Dental Association. 2018;149(10):859-868.
doi:10.1016/j.adaj.2018.05.015 .

Ćetković, Dejan, Antić, Svetlana, Antonijević, Đorđe, Brković, Božidar, Đukić, Ksenija, Vujašković, Goran, Đurić, Marija, "Nutrient canals and porosity of the bony palate A basis for the biological plausibility of the anterior middle superior alveolar nerve block" in Journal of the American Dental Association, 149, no. 10 (2018):859-868,
https://doi.org/10.1016/j.adaj.2018.05.015 . .

TY  - JOUR
AU  - Milosavljević, A.
AU  - Đukić, Ljiljana
AU  - Toljić, Boško
AU  - Milašin, Jelena
AU  - Dželetović, Bojan
AU  - Brković, Božidar
AU  - Roganović, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2321
AB  - AimTo investigate melatonin (MEL) levels in human dental pulp tissue (hDP) in type 2 diabetic (T2D) participants and the underlying molecular mechanisms of its effects in human dental pulp cells (hDPCs) under hyperglycaemia. MethodologyThe study included 16 healthy and 16 T2D participants who underwent vital pulp extirpation for hDP and four healthy participants undergoing third molar extraction for hDPCs analyses. MTT and NRU were used as tests for cytotoxicity. The pulp tissue levels of MEL, inducible NO synthase (iNOS) and superoxide dismutase (SOD) activity, as well as iNOS, histone acetyltransferase p300 (p300) and SOD activity levels in hDPCs incubated with MEL (0.1 and 1.0mmolL(-1)) under normoglycaemia and hyperglycaemia were measured by enzyme-linked immunosorbent assay. Comparisons between the two groups were made by unpaired t-tests or Mann-Whitney test whilst the chi-square test was used for dichotomous variables. To compare more groups, the Kruskal-Wallis test with Dunn's multiple comparison was used, whilst Spearman correlation was used to assess association between two variables. ResultsMelatonin was decreased (124.3021.6 vs. 240.0 +/- 19.1 pgmL(-1), P lt 0.01), whilst iNOS levels increased (0.92 +/- 0.08 vs. 0.32 +/- 0.09ngmL(-1), P lt 0.01) in hDP from T2D compared to nondiabetic participants. In hDPCs, MEL (0.1 and 1.0mmolL(-1)) had no cytotoxicity. Incubation with 1.0mmolL(-1) of MEL (24h) decreased hyperglycaemia-induced increases of iNOS (0.34 +/- 0.01ngmL(-1) vs. 0.40 +/- 0.01ngmL(-1), P lt 0.01) and p300 (11.59 +/- 0.58ngmL(-1) vs. 16.12 +/- 0.39ngmL(-1), P lt 0.01), and also, increased SOD activity (87.11 +/- 3.10% vs. 68.56 +/- 3.77%, P lt 0.01) to the levels comparable to the normoglycaemic; iNOS and p300 protein expression levels showed strong positive correlation under hyperglycaemia (Spearman r=0.8242, P lt 0.001). ConclusionType 2 diabetic participants had decreased MEL in hDP. At pharmacological concentrations, MEL is not cytotoxic for hDPCs and normalizes iNOS and SOD activity levels in hyperglyceamic hDPCs suggesting its antioxidant and protective effects in human dental pulp tissue under hyperglycaemia.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions
VL  - 51
IS  - 10
SP  - 1149
EP  - 1158
DO  - 10.1111/iej.12934
ER  - 

@article{
author = "Milosavljević, A. and Đukić, Ljiljana and Toljić, Boško and Milašin, Jelena and Dželetović, Bojan and Brković, Božidar and Roganović, Jelena",
year = "2018",
abstract = "AimTo investigate melatonin (MEL) levels in human dental pulp tissue (hDP) in type 2 diabetic (T2D) participants and the underlying molecular mechanisms of its effects in human dental pulp cells (hDPCs) under hyperglycaemia. MethodologyThe study included 16 healthy and 16 T2D participants who underwent vital pulp extirpation for hDP and four healthy participants undergoing third molar extraction for hDPCs analyses. MTT and NRU were used as tests for cytotoxicity. The pulp tissue levels of MEL, inducible NO synthase (iNOS) and superoxide dismutase (SOD) activity, as well as iNOS, histone acetyltransferase p300 (p300) and SOD activity levels in hDPCs incubated with MEL (0.1 and 1.0mmolL(-1)) under normoglycaemia and hyperglycaemia were measured by enzyme-linked immunosorbent assay. Comparisons between the two groups were made by unpaired t-tests or Mann-Whitney test whilst the chi-square test was used for dichotomous variables. To compare more groups, the Kruskal-Wallis test with Dunn's multiple comparison was used, whilst Spearman correlation was used to assess association between two variables. ResultsMelatonin was decreased (124.3021.6 vs. 240.0 +/- 19.1 pgmL(-1), P lt 0.01), whilst iNOS levels increased (0.92 +/- 0.08 vs. 0.32 +/- 0.09ngmL(-1), P lt 0.01) in hDP from T2D compared to nondiabetic participants. In hDPCs, MEL (0.1 and 1.0mmolL(-1)) had no cytotoxicity. Incubation with 1.0mmolL(-1) of MEL (24h) decreased hyperglycaemia-induced increases of iNOS (0.34 +/- 0.01ngmL(-1) vs. 0.40 +/- 0.01ngmL(-1), P lt 0.01) and p300 (11.59 +/- 0.58ngmL(-1) vs. 16.12 +/- 0.39ngmL(-1), P lt 0.01), and also, increased SOD activity (87.11 +/- 3.10% vs. 68.56 +/- 3.77%, P lt 0.01) to the levels comparable to the normoglycaemic; iNOS and p300 protein expression levels showed strong positive correlation under hyperglycaemia (Spearman r=0.8242, P lt 0.001). ConclusionType 2 diabetic participants had decreased MEL in hDP. At pharmacological concentrations, MEL is not cytotoxic for hDPCs and normalizes iNOS and SOD activity levels in hyperglyceamic hDPCs suggesting its antioxidant and protective effects in human dental pulp tissue under hyperglycaemia.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions",
volume = "51",
number = "10",
pages = "1149-1158",
doi = "10.1111/iej.12934"
}

Milosavljević, A., Đukić, L., Toljić, B., Milašin, J., Dželetović, B., Brković, B.,& Roganović, J.. (2018). Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions. in International Endodontic Journal
Wiley, Hoboken., 51(10), 1149-1158.
https://doi.org/10.1111/iej.12934

Milosavljević A, Đukić L, Toljić B, Milašin J, Dželetović B, Brković B, Roganović J. Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions. in International Endodontic Journal. 2018;51(10):1149-1158.
doi:10.1111/iej.12934 .

Milosavljević, A., Đukić, Ljiljana, Toljić, Boško, Milašin, Jelena, Dželetović, Bojan, Brković, Božidar, Roganović, Jelena, "Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions" in International Endodontic Journal, 51, no. 10 (2018):1149-1158,
https://doi.org/10.1111/iej.12934 . .

TY  - JOUR
AU  - Radović, Nikola
AU  - Nikolić-Jakoba, Nataša
AU  - Petrović, Nina
AU  - Milosavljević, Aleksandra
AU  - Brković, Božidar
AU  - Roganović, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2519
AB  - AimRecent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively). Material and methodsmiR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically. ResultsThe upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender. ConclusionsmiR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients.
PB  - Wiley, Hoboken
T2  - Journal of Clinical Periodontology
T1  - MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients
VL  - 45
IS  - 6
SP  - 663
EP  - 671
DO  - 10.1111/jcpe.12888
ER  - 

@article{
author = "Radović, Nikola and Nikolić-Jakoba, Nataša and Petrović, Nina and Milosavljević, Aleksandra and Brković, Božidar and Roganović, Jelena",
year = "2018",
abstract = "AimRecent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively). Material and methodsmiR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically. ResultsThe upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender. ConclusionsmiR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients.",
publisher = "Wiley, Hoboken",
journal = "Journal of Clinical Periodontology",
title = "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients",
volume = "45",
number = "6",
pages = "663-671",
doi = "10.1111/jcpe.12888"
}

Radović, N., Nikolić-Jakoba, N., Petrović, N., Milosavljević, A., Brković, B.,& Roganović, J.. (2018). MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. in Journal of Clinical Periodontology
Wiley, Hoboken., 45(6), 663-671.
https://doi.org/10.1111/jcpe.12888

Radović N, Nikolić-Jakoba N, Petrović N, Milosavljević A, Brković B, Roganović J. MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. in Journal of Clinical Periodontology. 2018;45(6):663-671.
doi:10.1111/jcpe.12888 .

Radović, Nikola, Nikolić-Jakoba, Nataša, Petrović, Nina, Milosavljević, Aleksandra, Brković, Božidar, Roganović, Jelena, "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients" in Journal of Clinical Periodontology, 45, no. 6 (2018):663-671,
https://doi.org/10.1111/jcpe.12888 . .

TY  - JOUR
AU  - Radović, Nikola
AU  - Nikolić-Jakoba, Nataša
AU  - Petrović, Nina
AU  - Milosavljević, Aleksandra
AU  - Brković, Božidar
AU  - Roganović, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2282
AB  - AimRecent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively). Material and methodsmiR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically. ResultsThe upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender. ConclusionsmiR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients.
PB  - Wiley, Hoboken
T2  - Journal of Clinical Periodontology
T1  - MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients
VL  - 45
IS  - 6
SP  - 663
EP  - 671
DO  - 10.1111/jcpe.12888
ER  - 

@article{
author = "Radović, Nikola and Nikolić-Jakoba, Nataša and Petrović, Nina and Milosavljević, Aleksandra and Brković, Božidar and Roganović, Jelena",
year = "2018",
abstract = "AimRecent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively). Material and methodsmiR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically. ResultsThe upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender. ConclusionsmiR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients.",
publisher = "Wiley, Hoboken",
journal = "Journal of Clinical Periodontology",
title = "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients",
volume = "45",
number = "6",
pages = "663-671",
doi = "10.1111/jcpe.12888"
}

Radović, N., Nikolić-Jakoba, N., Petrović, N., Milosavljević, A., Brković, B.,& Roganović, J.. (2018). MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. in Journal of Clinical Periodontology
Wiley, Hoboken., 45(6), 663-671.
https://doi.org/10.1111/jcpe.12888

Radović N, Nikolić-Jakoba N, Petrović N, Milosavljević A, Brković B, Roganović J. MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. in Journal of Clinical Periodontology. 2018;45(6):663-671.
doi:10.1111/jcpe.12888 .

Radović, Nikola, Nikolić-Jakoba, Nataša, Petrović, Nina, Milosavljević, Aleksandra, Brković, Božidar, Roganović, Jelena, "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients" in Journal of Clinical Periodontology, 45, no. 6 (2018):663-671,
https://doi.org/10.1111/jcpe.12888 . .

TY  - JOUR
AU  - Biočanin, Vladimir
AU  - Antonijević, Đorđe
AU  - Poštić, Srđan
AU  - Ilić, Dragan
AU  - Vuković, Zorica
AU  - Milić, Marija
AU  - Fan, Yifang
AU  - Li, Zhiyu
AU  - Brković, Božidar
AU  - Durić, Marija
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2289
AB  - Introduction: The outcome of periapical surgery has been directly improved with the introduction of novel material formulations. The aim of the study was to compare the retrograde obturation quality of the following materials: calcium silicate (Biodentine; Septodont, Saint-Maur-des-Fosses, France), mineral trioxide aggregate (MTA+; Cerkamed Company, Stalowa Wola, Poland), and glass ionomer cement (Fuji IX; GC Corporation, Tokyo, Japan). Methods: Materials' wettability was calculated concerning the contact angles of the cements measured using a glycerol drop. Cements' porosity was determined using mercury intrusion porosimetry and micro computed tomographic (mu CT) imaging. Extracted upper human incisors were retrofilled, and mu CT analysis was applied to calculate the volume of the gap between the retrograde filling material and root canal dentin. Experiments were performed before and after soaking the materials in simulated body fluid (SBF). Results: No statistically significant differences were found among the contact angles of the studied materials after being soaked in SBF. The material with the lowest nanoporosity (Fuji IX: 2.99% and 4.17% before and after SBF, respectively) showed the highest values of microporosity (4.2% and 3.1% before and after SBF, respectively). Biodentine had the lowest value of microporosity (1.2% and 0.8% before and after SBF, respectively) and the lowest value of microgap to the root canal wall ([10 +/- 30] x 10(-3) mm(3)). Conclusions: Biodentine and MTA possess certain advantages over Fuji IX for hermetic obturation of retrograde root canals. Biodentine shows a tendency toward the lowest marginal gap at the cement-to-dentin interface.
PB  - Elsevier Science Inc, New York
T2  - Journal of Endodontics
T1  - Marginal Gaps between 2 Calcium Silicate and Glass lonomer Cements and Apical Root Dentin
VL  - 44
IS  - 5
SP  - 816
EP  - 821
DO  - 10.1016/j.joen.2017.09.022
ER  - 

@article{
author = "Biočanin, Vladimir and Antonijević, Đorđe and Poštić, Srđan and Ilić, Dragan and Vuković, Zorica and Milić, Marija and Fan, Yifang and Li, Zhiyu and Brković, Božidar and Durić, Marija",
year = "2018",
abstract = "Introduction: The outcome of periapical surgery has been directly improved with the introduction of novel material formulations. The aim of the study was to compare the retrograde obturation quality of the following materials: calcium silicate (Biodentine; Septodont, Saint-Maur-des-Fosses, France), mineral trioxide aggregate (MTA+; Cerkamed Company, Stalowa Wola, Poland), and glass ionomer cement (Fuji IX; GC Corporation, Tokyo, Japan). Methods: Materials' wettability was calculated concerning the contact angles of the cements measured using a glycerol drop. Cements' porosity was determined using mercury intrusion porosimetry and micro computed tomographic (mu CT) imaging. Extracted upper human incisors were retrofilled, and mu CT analysis was applied to calculate the volume of the gap between the retrograde filling material and root canal dentin. Experiments were performed before and after soaking the materials in simulated body fluid (SBF). Results: No statistically significant differences were found among the contact angles of the studied materials after being soaked in SBF. The material with the lowest nanoporosity (Fuji IX: 2.99% and 4.17% before and after SBF, respectively) showed the highest values of microporosity (4.2% and 3.1% before and after SBF, respectively). Biodentine had the lowest value of microporosity (1.2% and 0.8% before and after SBF, respectively) and the lowest value of microgap to the root canal wall ([10 +/- 30] x 10(-3) mm(3)). Conclusions: Biodentine and MTA possess certain advantages over Fuji IX for hermetic obturation of retrograde root canals. Biodentine shows a tendency toward the lowest marginal gap at the cement-to-dentin interface.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Endodontics",
title = "Marginal Gaps between 2 Calcium Silicate and Glass lonomer Cements and Apical Root Dentin",
volume = "44",
number = "5",
pages = "816-821",
doi = "10.1016/j.joen.2017.09.022"
}

Biočanin, V., Antonijević, Đ., Poštić, S., Ilić, D., Vuković, Z., Milić, M., Fan, Y., Li, Z., Brković, B.,& Durić, M.. (2018). Marginal Gaps between 2 Calcium Silicate and Glass lonomer Cements and Apical Root Dentin. in Journal of Endodontics
Elsevier Science Inc, New York., 44(5), 816-821.
https://doi.org/10.1016/j.joen.2017.09.022

Biočanin V, Antonijević Đ, Poštić S, Ilić D, Vuković Z, Milić M, Fan Y, Li Z, Brković B, Durić M. Marginal Gaps between 2 Calcium Silicate and Glass lonomer Cements and Apical Root Dentin. in Journal of Endodontics. 2018;44(5):816-821.
doi:10.1016/j.joen.2017.09.022 .

Biočanin, Vladimir, Antonijević, Đorđe, Poštić, Srđan, Ilić, Dragan, Vuković, Zorica, Milić, Marija, Fan, Yifang, Li, Zhiyu, Brković, Božidar, Durić, Marija, "Marginal Gaps between 2 Calcium Silicate and Glass lonomer Cements and Apical Root Dentin" in Journal of Endodontics, 44, no. 5 (2018):816-821,
https://doi.org/10.1016/j.joen.2017.09.022 . .

TY  - JOUR
AU  - Ležaja-Zebić, Maja
AU  - Dželetović, Bojan
AU  - Miletić, Vesna
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2299
AB  - Objectives: The aim of this study was to test long-term microtensile bond strength (mu TBS) of 2 universal adhesives applied to dentin following "total-etch" (TE) or "self-etch" (SE) protocols and aged by direct or indirect water exposure using simulated pulpal pressure. Materials and methods: Single Bond Universal (SBU, 3M ESPE) or Ipera Bond (IP, Itena) were applied to mid-coronal dentin ("flat dentin") or Class I cavity ("cavity dentin") following TE or SE protocols in 112 extracted human third molars. Sixteen groups (n=7 per group) were prepared, 8 groups for mu TBS measurements after 24 hours, and further 8 groups for measurements after 6 months storage. "Cavity dentin" groups were subjected to simulated hydrostatic pulpal pressure of 15 cm H2O using a custom-made device before cutting 1 x 1 mm sticks while "flat dentin" groups were cut into sticks and directly exposed to deionized water. Results: Generally, the TE protocol resulted in highest mu TBS values on flat dentin initially for both adhesives (general linear model, P lt .05). Long-term storage resulted in significantly lower mu TBS values for the TE protocol (P lt .05) while the SE protocol showed comparable values after 6 months (P>.05). "Cavity dentin" with simulated pulpal pressure resulted in lower mu TBS than "flat dentin" (P lt .05). For both adhesives, mu TBS was in the range of 19-42 MPa initially and 16-36 MPa after 6 months storage. Conclusions: mu TBS to dentin of universal adhesives is more stable in the long term following the SE than TE protocol. Simulated pulpal pressure and cavity-type sample preparation may be recommended for mu TBS testing as a more clinically relevant strategy.
PB  - Wiley, Hoboken
T2  - Journal of Esthetic & Restorative Dentistry
T1  - Microtensile bond strength of universal adhesives to flat versus Class I cavity dentin with pulpal pressure simulation
VL  - 30
IS  - 3
SP  - 240
EP  - 248
DO  - 10.1111/jerd.12363
ER  - 

@article{
author = "Ležaja-Zebić, Maja and Dželetović, Bojan and Miletić, Vesna",
year = "2018",
abstract = "Objectives: The aim of this study was to test long-term microtensile bond strength (mu TBS) of 2 universal adhesives applied to dentin following "total-etch" (TE) or "self-etch" (SE) protocols and aged by direct or indirect water exposure using simulated pulpal pressure. Materials and methods: Single Bond Universal (SBU, 3M ESPE) or Ipera Bond (IP, Itena) were applied to mid-coronal dentin ("flat dentin") or Class I cavity ("cavity dentin") following TE or SE protocols in 112 extracted human third molars. Sixteen groups (n=7 per group) were prepared, 8 groups for mu TBS measurements after 24 hours, and further 8 groups for measurements after 6 months storage. "Cavity dentin" groups were subjected to simulated hydrostatic pulpal pressure of 15 cm H2O using a custom-made device before cutting 1 x 1 mm sticks while "flat dentin" groups were cut into sticks and directly exposed to deionized water. Results: Generally, the TE protocol resulted in highest mu TBS values on flat dentin initially for both adhesives (general linear model, P lt .05). Long-term storage resulted in significantly lower mu TBS values for the TE protocol (P lt .05) while the SE protocol showed comparable values after 6 months (P>.05). "Cavity dentin" with simulated pulpal pressure resulted in lower mu TBS than "flat dentin" (P lt .05). For both adhesives, mu TBS was in the range of 19-42 MPa initially and 16-36 MPa after 6 months storage. Conclusions: mu TBS to dentin of universal adhesives is more stable in the long term following the SE than TE protocol. Simulated pulpal pressure and cavity-type sample preparation may be recommended for mu TBS testing as a more clinically relevant strategy.",
publisher = "Wiley, Hoboken",
journal = "Journal of Esthetic & Restorative Dentistry",
title = "Microtensile bond strength of universal adhesives to flat versus Class I cavity dentin with pulpal pressure simulation",
volume = "30",
number = "3",
pages = "240-248",
doi = "10.1111/jerd.12363"
}

Ležaja-Zebić, M., Dželetović, B.,& Miletić, V.. (2018). Microtensile bond strength of universal adhesives to flat versus Class I cavity dentin with pulpal pressure simulation. in Journal of Esthetic & Restorative Dentistry
Wiley, Hoboken., 30(3), 240-248.
https://doi.org/10.1111/jerd.12363

Ležaja-Zebić M, Dželetović B, Miletić V. Microtensile bond strength of universal adhesives to flat versus Class I cavity dentin with pulpal pressure simulation. in Journal of Esthetic & Restorative Dentistry. 2018;30(3):240-248.
doi:10.1111/jerd.12363 .

Ležaja-Zebić, Maja, Dželetović, Bojan, Miletić, Vesna, "Microtensile bond strength of universal adhesives to flat versus Class I cavity dentin with pulpal pressure simulation" in Journal of Esthetic & Restorative Dentistry, 30, no. 3 (2018):240-248,
https://doi.org/10.1111/jerd.12363 . .

TY  - JOUR
AU  - Brković, Božidar
AU  - Andrić, Miroslav
AU  - Ćalasan, Dejan
AU  - Milić, Marija
AU  - Stepić, Jelena
AU  - Vučetić, Milan
AU  - Brajković, Denis
AU  - Todorović, Ljubomir
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2241
AB  - The purpose of this study was to investigate postoperative analgesic effect of ropivacaine administered as main or supplemental injection for the inferior alveolar nerve block (IANB) in patients undergoing lower third molar surgery. The double-blind randomized study comprised 72 healthy patients. All patients received two blocks, the IANB for surgical procedure + IANB after surgery for postoperative pain control, and were divided into three groups: (1) 2 % lidocaine/epinephrine + 1 % ropivacaine, (2) 2 % lidocaine/epinephrine + saline, and (3) 1 % ropivacaine + saline. The occurrence of postoperative pain, pain intensity and analgesic requirements were recorded. Data were statistically analyzed using chi-square, Fisher, and Kruskal-Wallis tests and analysis of variance (ANOVA) with Bonferroni and Tukey correction. Ropivacaine was more successful than lidocaine/epinephrine in obtaining duration of postoperative analgesia, reduction of pain, and analgesic requirements whether ropivacaine was used for surgical block or administered as a supplemental injection after surgery. Ropivacaine (1 %, 2 ml) resulted in effective postoperative analgesia after lower third molar surgery. Since pain control related to third molar surgery requires the effective surgical anesthesia and postoperative analgesia, the use of 1 % ropivacaine could be clinically relevant in a selection of appropriate pain control regimen for both surgical procedure and early postsurgical treatment.
PB  - Springer Heidelberg, Heidelberg
T2  - Clinical Oral Investigations
T1  - Efficacy and safety of 1% ropivacaine for postoperative analgesia after lower third molar surgery: a prospective, randomized, double-blinded clinical study
VL  - 21
IS  - 3
SP  - 779
EP  - 785
DO  - 10.1007/s00784-016-1831-2
ER  - 

@article{
author = "Brković, Božidar and Andrić, Miroslav and Ćalasan, Dejan and Milić, Marija and Stepić, Jelena and Vučetić, Milan and Brajković, Denis and Todorović, Ljubomir",
year = "2017",
abstract = "The purpose of this study was to investigate postoperative analgesic effect of ropivacaine administered as main or supplemental injection for the inferior alveolar nerve block (IANB) in patients undergoing lower third molar surgery. The double-blind randomized study comprised 72 healthy patients. All patients received two blocks, the IANB for surgical procedure + IANB after surgery for postoperative pain control, and were divided into three groups: (1) 2 % lidocaine/epinephrine + 1 % ropivacaine, (2) 2 % lidocaine/epinephrine + saline, and (3) 1 % ropivacaine + saline. The occurrence of postoperative pain, pain intensity and analgesic requirements were recorded. Data were statistically analyzed using chi-square, Fisher, and Kruskal-Wallis tests and analysis of variance (ANOVA) with Bonferroni and Tukey correction. Ropivacaine was more successful than lidocaine/epinephrine in obtaining duration of postoperative analgesia, reduction of pain, and analgesic requirements whether ropivacaine was used for surgical block or administered as a supplemental injection after surgery. Ropivacaine (1 %, 2 ml) resulted in effective postoperative analgesia after lower third molar surgery. Since pain control related to third molar surgery requires the effective surgical anesthesia and postoperative analgesia, the use of 1 % ropivacaine could be clinically relevant in a selection of appropriate pain control regimen for both surgical procedure and early postsurgical treatment.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Clinical Oral Investigations",
title = "Efficacy and safety of 1% ropivacaine for postoperative analgesia after lower third molar surgery: a prospective, randomized, double-blinded clinical study",
volume = "21",
number = "3",
pages = "779-785",
doi = "10.1007/s00784-016-1831-2"
}

Brković, B., Andrić, M., Ćalasan, D., Milić, M., Stepić, J., Vučetić, M., Brajković, D.,& Todorović, L.. (2017). Efficacy and safety of 1% ropivacaine for postoperative analgesia after lower third molar surgery: a prospective, randomized, double-blinded clinical study. in Clinical Oral Investigations
Springer Heidelberg, Heidelberg., 21(3), 779-785.
https://doi.org/10.1007/s00784-016-1831-2

Brković B, Andrić M, Ćalasan D, Milić M, Stepić J, Vučetić M, Brajković D, Todorović L. Efficacy and safety of 1% ropivacaine for postoperative analgesia after lower third molar surgery: a prospective, randomized, double-blinded clinical study. in Clinical Oral Investigations. 2017;21(3):779-785.
doi:10.1007/s00784-016-1831-2 .

Brković, Božidar, Andrić, Miroslav, Ćalasan, Dejan, Milić, Marija, Stepić, Jelena, Vučetić, Milan, Brajković, Denis, Todorović, Ljubomir, "Efficacy and safety of 1% ropivacaine for postoperative analgesia after lower third molar surgery: a prospective, randomized, double-blinded clinical study" in Clinical Oral Investigations, 21, no. 3 (2017):779-785,
https://doi.org/10.1007/s00784-016-1831-2 . .

TY  - JOUR
AU  - Kosanić, Ivan
AU  - Andrić, Miroslav
AU  - Brković, Božidar
AU  - Koković, Vladimir
AU  - Jurišić, Milan
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2233
AB  - Background/Aim. The use of autogenous bone grafts for augmentation of the resorbed alveolar ridge is still considered the gold standard in implant dentistry. The aim of this study was to analyze the resorption rate of autogenous bone block grafts from the retromolar region placed in the frontal segment of the upper jaw unprotected by barrier membranes, to assess the stability of implants placed into the grafted bone, as well as to monitor its changes during the healing period. Methods. The study included 18 patients with a total of 20 grafted sites. The residual alveolar ridge was measured before and after the augmentation and prior to implant placement. All implants were restored with provisional crowns within 48 hours after the placement. Implant stability was assessed using resonance frequency analysis. Results. The average period from ridge augmentation to reentry was 5.4 months (range 4-6 months) . At reentry the healed alveolar ridge had a mean width of 6.1 ± 1.27 mm. The mean calculated width gain was 3.04 ± 1.22 mm. The overall surface resorption of block grafts was 0.68 ± 0.69 mm (18.85%). At the time of implant placement the mean value of implant stability quotient (ISQ) was 71.25 ± 5.77. The lowest ISQ values were noted after three weeks of healing, followed by a gradual increase until week 12. After 12 weeks implants showed significantly higher ISQ values compared to primary stability (p  lt  0.05 Wilcoxon signed ranks test). During the 3-years followup period no cases of implant loss were recorded. Conclusion. Despite a significant resorption of bone grafts, it was possible to place implants in all the cases and to use the immediate loading protocol without affecting implant survival rate.
AB  - Uvod/cilj. Primena autogenih koštanih graftova (implantata) za uvećavanje smanjenog (resorbovanog) alvelarnog grebena još uvek se smatra zlatnim standardom u implantologiji. Cilj ove studije bio je analiza stepena resorpcije autolognih koštanih blok transplantata nezaštićenih barijernim mebranama, uzetih iz retromolarnog predela mandibule i postavljenih u frontalni segment gornje vilice, procena stabilnosti implantata ugrađenih u povećanu regiju, kao i praćenje promene implantne stabilnosti tokom perioda oseointegracije. Metode. U studiju je bilo uključeno 18 pacijenata sa ukupno 20 autotransplantata. Širina rezidualnog alveolarnog grebena merena je pre i posle postavljanja transplantata, kao i neposredno pre ugradnje implantata. Svi implantati su opterećeni privremenim nadoknadama 48 sati nakon ugradnje. Stabilnost implantata procenjivana je primenom analize rezonantne frekvencije. Rezultati. Srednje vreme između uvećavanja grebena i ugradnje implantata iznosilo je 5,4 (4-6) meseci. Pre ugradnje implantata srednja vrednost širine grebena iznosila je 6,1 ± 1,27 mm, a povećanja širine grebena u odnosu na vrednosti pre uvećanja 3,04 ± 1,22 mm. Površinska resoprcija grafta iznosila je 18,85% (0,68 ± 0,69 mm). Srednja vrednost koeficijenta implantne stabilnosti (ISQ) u momentu ugradnje iznosila je 71,25 ± 5,77. Najniže vrednosti ISQ zabeležene su u trećoj nedelji nakon ugradnje, što je bilo praćeno postepenim porastom do dvanaeste nedelje zarastanja. Nakon dvanaest nedelja vrednosti ISQ bile su statitički značajno više od vrednosti u momentu ugradnje (p  lt  0,05 Wilcoxon test). Tokom trogodišnjeg perioda praćenja nije bilo izgubljenih implantata. Zaključak. Bez obzira na značajan stepen resorpcije autotransplantata, kod svih pacijenata bilo je moguće ugraditi implantate u uvećani greben, kao i primeniti protokol ranog opterećenja bez uticaja na stepen preživljavanja implantata.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Unprotected autogenous bone block grafts in the anterior maxilla: Resorption rates and clinical outcomes
T1  - Nezaštićeni koštani autotransplantati u prednjem segmentu gornje vilice - stepen resorpcije i klinički rezultati
VL  - 74
IS  - 4
SP  - 305
EP  - 310
DO  - 10.2298/VSP150429188K
ER  - 

@article{
author = "Kosanić, Ivan and Andrić, Miroslav and Brković, Božidar and Koković, Vladimir and Jurišić, Milan",
year = "2017",
abstract = "Background/Aim. The use of autogenous bone grafts for augmentation of the resorbed alveolar ridge is still considered the gold standard in implant dentistry. The aim of this study was to analyze the resorption rate of autogenous bone block grafts from the retromolar region placed in the frontal segment of the upper jaw unprotected by barrier membranes, to assess the stability of implants placed into the grafted bone, as well as to monitor its changes during the healing period. Methods. The study included 18 patients with a total of 20 grafted sites. The residual alveolar ridge was measured before and after the augmentation and prior to implant placement. All implants were restored with provisional crowns within 48 hours after the placement. Implant stability was assessed using resonance frequency analysis. Results. The average period from ridge augmentation to reentry was 5.4 months (range 4-6 months) . At reentry the healed alveolar ridge had a mean width of 6.1 ± 1.27 mm. The mean calculated width gain was 3.04 ± 1.22 mm. The overall surface resorption of block grafts was 0.68 ± 0.69 mm (18.85%). At the time of implant placement the mean value of implant stability quotient (ISQ) was 71.25 ± 5.77. The lowest ISQ values were noted after three weeks of healing, followed by a gradual increase until week 12. After 12 weeks implants showed significantly higher ISQ values compared to primary stability (p  lt  0.05 Wilcoxon signed ranks test). During the 3-years followup period no cases of implant loss were recorded. Conclusion. Despite a significant resorption of bone grafts, it was possible to place implants in all the cases and to use the immediate loading protocol without affecting implant survival rate., Uvod/cilj. Primena autogenih koštanih graftova (implantata) za uvećavanje smanjenog (resorbovanog) alvelarnog grebena još uvek se smatra zlatnim standardom u implantologiji. Cilj ove studije bio je analiza stepena resorpcije autolognih koštanih blok transplantata nezaštićenih barijernim mebranama, uzetih iz retromolarnog predela mandibule i postavljenih u frontalni segment gornje vilice, procena stabilnosti implantata ugrađenih u povećanu regiju, kao i praćenje promene implantne stabilnosti tokom perioda oseointegracije. Metode. U studiju je bilo uključeno 18 pacijenata sa ukupno 20 autotransplantata. Širina rezidualnog alveolarnog grebena merena je pre i posle postavljanja transplantata, kao i neposredno pre ugradnje implantata. Svi implantati su opterećeni privremenim nadoknadama 48 sati nakon ugradnje. Stabilnost implantata procenjivana je primenom analize rezonantne frekvencije. Rezultati. Srednje vreme između uvećavanja grebena i ugradnje implantata iznosilo je 5,4 (4-6) meseci. Pre ugradnje implantata srednja vrednost širine grebena iznosila je 6,1 ± 1,27 mm, a povećanja širine grebena u odnosu na vrednosti pre uvećanja 3,04 ± 1,22 mm. Površinska resoprcija grafta iznosila je 18,85% (0,68 ± 0,69 mm). Srednja vrednost koeficijenta implantne stabilnosti (ISQ) u momentu ugradnje iznosila je 71,25 ± 5,77. Najniže vrednosti ISQ zabeležene su u trećoj nedelji nakon ugradnje, što je bilo praćeno postepenim porastom do dvanaeste nedelje zarastanja. Nakon dvanaest nedelja vrednosti ISQ bile su statitički značajno više od vrednosti u momentu ugradnje (p  lt  0,05 Wilcoxon test). Tokom trogodišnjeg perioda praćenja nije bilo izgubljenih implantata. Zaključak. Bez obzira na značajan stepen resorpcije autotransplantata, kod svih pacijenata bilo je moguće ugraditi implantate u uvećani greben, kao i primeniti protokol ranog opterećenja bez uticaja na stepen preživljavanja implantata.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Unprotected autogenous bone block grafts in the anterior maxilla: Resorption rates and clinical outcomes, Nezaštićeni koštani autotransplantati u prednjem segmentu gornje vilice - stepen resorpcije i klinički rezultati",
volume = "74",
number = "4",
pages = "305-310",
doi = "10.2298/VSP150429188K"
}

Kosanić, I., Andrić, M., Brković, B., Koković, V.,& Jurišić, M.. (2017). Unprotected autogenous bone block grafts in the anterior maxilla: Resorption rates and clinical outcomes. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 74(4), 305-310.
https://doi.org/10.2298/VSP150429188K

Kosanić I, Andrić M, Brković B, Koković V, Jurišić M. Unprotected autogenous bone block grafts in the anterior maxilla: Resorption rates and clinical outcomes. in Vojnosanitetski pregled. 2017;74(4):305-310.
doi:10.2298/VSP150429188K .

Kosanić, Ivan, Andrić, Miroslav, Brković, Božidar, Koković, Vladimir, Jurišić, Milan, "Unprotected autogenous bone block grafts in the anterior maxilla: Resorption rates and clinical outcomes" in Vojnosanitetski pregled, 74, no. 4 (2017):305-310,
https://doi.org/10.2298/VSP150429188K . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Davidović, Radoslav
AU  - Jovanović-Cupić, Snezana
AU  - Krajnović, Milena
AU  - Lukić, Silvana
AU  - Petrović, Milan
AU  - Roganović, Jelena
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2120
AB  - Breast cancer (BC) is a heterogeneous group of diseases that still represents a major cause of death in the female population. MicroRNAs (miRNAs, miRs), such as miR-221 and miR-222, have been shown to be involved in BC pathology by acting via its target genes such as tissue inhibitor of metalloproteinase 3 (TIMP3). The main goals of this study were to find differences in miR-221/222 levels of expression in BC groups based on invasiveness, and to investigate the association with estrogen receptor (ER), TIMP3 messenger RNA (mRNA) levels, and clinicopathological characteristics of patients and tumors. In this study, we measured levels of miR-221/222 in 63 breast tissue samples by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) using TaqMan(A (R)) technology and immunohistochemistry. miR-221/222 levels varied significantly across groups based on invasiveness (P  lt  0.001). In in situ tumors, miR-221 and miR-222 were negatively associated with ER (P = 0.001, r = -0.714, and P = 0.013, r = -0.585, respectively). In invasive breast carcinomas associated with non-invasive tumors, miR-222 was inversely associated with ER (P = 0.039, r = -0.620). Pure invasive BCs showed a positive correlation of miR-221 and miR-222 with TIMP3 mRNA levels (P = 0.008, r = 0.508, and P = 0.010, r = 0.497, respectively). An increase in miR-221/222 might be an important event for in situ carcinoma formation, and miR-221/222 may be important molecules that highlight potential differences between invasive breast carcinomas associated with non-invasive and pure invasive BCs.
PB  - Adis Int Ltd, Northcote
T2  - Molecular Diagnosis & Therapy
T1  - Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels
VL  - 20
IS  - 6
SP  - 603
EP  - 615
DO  - 10.1007/s40291-016-0230-3
ER  - 

@article{
author = "Petrović, Nina and Davidović, Radoslav and Jovanović-Cupić, Snezana and Krajnović, Milena and Lukić, Silvana and Petrović, Milan and Roganović, Jelena",
year = "2016",
abstract = "Breast cancer (BC) is a heterogeneous group of diseases that still represents a major cause of death in the female population. MicroRNAs (miRNAs, miRs), such as miR-221 and miR-222, have been shown to be involved in BC pathology by acting via its target genes such as tissue inhibitor of metalloproteinase 3 (TIMP3). The main goals of this study were to find differences in miR-221/222 levels of expression in BC groups based on invasiveness, and to investigate the association with estrogen receptor (ER), TIMP3 messenger RNA (mRNA) levels, and clinicopathological characteristics of patients and tumors. In this study, we measured levels of miR-221/222 in 63 breast tissue samples by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) using TaqMan(A (R)) technology and immunohistochemistry. miR-221/222 levels varied significantly across groups based on invasiveness (P  lt  0.001). In in situ tumors, miR-221 and miR-222 were negatively associated with ER (P = 0.001, r = -0.714, and P = 0.013, r = -0.585, respectively). In invasive breast carcinomas associated with non-invasive tumors, miR-222 was inversely associated with ER (P = 0.039, r = -0.620). Pure invasive BCs showed a positive correlation of miR-221 and miR-222 with TIMP3 mRNA levels (P = 0.008, r = 0.508, and P = 0.010, r = 0.497, respectively). An increase in miR-221/222 might be an important event for in situ carcinoma formation, and miR-221/222 may be important molecules that highlight potential differences between invasive breast carcinomas associated with non-invasive and pure invasive BCs.",
publisher = "Adis Int Ltd, Northcote",
journal = "Molecular Diagnosis & Therapy",
title = "Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels",
volume = "20",
number = "6",
pages = "603-615",
doi = "10.1007/s40291-016-0230-3"
}

Petrović, N., Davidović, R., Jovanović-Cupić, S., Krajnović, M., Lukić, S., Petrović, M.,& Roganović, J.. (2016). Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels. in Molecular Diagnosis & Therapy
Adis Int Ltd, Northcote., 20(6), 603-615.
https://doi.org/10.1007/s40291-016-0230-3

Petrović N, Davidović R, Jovanović-Cupić S, Krajnović M, Lukić S, Petrović M, Roganović J. Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels. in Molecular Diagnosis & Therapy. 2016;20(6):603-615.
doi:10.1007/s40291-016-0230-3 .

Petrović, Nina, Davidović, Radoslav, Jovanović-Cupić, Snezana, Krajnović, Milena, Lukić, Silvana, Petrović, Milan, Roganović, Jelena, "Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels" in Molecular Diagnosis & Therapy, 20, no. 6 (2016):603-615,
https://doi.org/10.1007/s40291-016-0230-3 . .

TY  - JOUR
AU  - Ilić, Jugoslav
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2107
AB  - Anatomically, dental pulp is connective tissue and specific microcirculatory system with significant reparatory abilities intending to preserve pulp vitality. Various therapeutic approaches in the treatment of affected pulp may be compromised by various factors leading to treatment failure. Due to microcirculatory system disorders, treatment of affected dental pulp in patients with diabetes mellitus (DM) is additional challenge. The function and levels of growth factors could be altered in various diabetic tissues including dental pulp. Among them are growth factors important for reparative response of the pulp. There are experimental evidences that DM impede dental pulp reparation. Therefore, clinical procedures aiming to preserve vitality of diabetic dental pulp should be applied with caution. The aim of this paper is to present basic factors and parameters that affect reparative response of dental pulp in patients with DM.
AB  - Zubna pulpa je vezivno tkivo i poseban mikrocirkulatorni sistem sa značajnim reparatornim sposobnostima u cilju očuvanja njenog vitaliteta. Terapijski postupci u lečenju obolele pulpe mogu da budu kompromitovani delovanjem brojnih faktora koji mogu uticati na ishod lečenja. Zubna pulpa kod pacijenata sa dijabetes melitusom (DM), zbog mikrocirkulatornih poremećaja često predstavlja poseban terapijski izazov. Poznato je da funkcije i nivoi faktora rasta mogu biti promenjeni u dijabetičnim tkivima, pa samim tim i u zubnoj pulpi. Ovde su od najveće važnosti faktori rasta značajni za regulaciju reparatornog odgovora pulpe. Postoje eksperimentalni dokazi da DM umanjuje reparativnu aktivnost zubne pulpe, pa zbog toga kliničke procedure vezane za očuvanje vitaliteta dijabetične zubne pulpe treba realizovati uz značajne mere opreza. Cilj ovog rada je da se predstave osnovni faktori i parametri koji utiču na reparatorni odgovor pulpe zuba kod obolelih od DM.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Diabetes mellitus and reparative response of dental pulp
T1  - Dijabetes melitus i reparativni odgovor zubne pulpe
VL  - 63
IS  - 2
SP  - 85
EP  - 90
DO  - 10.1515/sdj-2016-0009
ER  - 

@article{
author = "Ilić, Jugoslav",
year = "2016",
abstract = "Anatomically, dental pulp is connective tissue and specific microcirculatory system with significant reparatory abilities intending to preserve pulp vitality. Various therapeutic approaches in the treatment of affected pulp may be compromised by various factors leading to treatment failure. Due to microcirculatory system disorders, treatment of affected dental pulp in patients with diabetes mellitus (DM) is additional challenge. The function and levels of growth factors could be altered in various diabetic tissues including dental pulp. Among them are growth factors important for reparative response of the pulp. There are experimental evidences that DM impede dental pulp reparation. Therefore, clinical procedures aiming to preserve vitality of diabetic dental pulp should be applied with caution. The aim of this paper is to present basic factors and parameters that affect reparative response of dental pulp in patients with DM., Zubna pulpa je vezivno tkivo i poseban mikrocirkulatorni sistem sa značajnim reparatornim sposobnostima u cilju očuvanja njenog vitaliteta. Terapijski postupci u lečenju obolele pulpe mogu da budu kompromitovani delovanjem brojnih faktora koji mogu uticati na ishod lečenja. Zubna pulpa kod pacijenata sa dijabetes melitusom (DM), zbog mikrocirkulatornih poremećaja često predstavlja poseban terapijski izazov. Poznato je da funkcije i nivoi faktora rasta mogu biti promenjeni u dijabetičnim tkivima, pa samim tim i u zubnoj pulpi. Ovde su od najveće važnosti faktori rasta značajni za regulaciju reparatornog odgovora pulpe. Postoje eksperimentalni dokazi da DM umanjuje reparativnu aktivnost zubne pulpe, pa zbog toga kliničke procedure vezane za očuvanje vitaliteta dijabetične zubne pulpe treba realizovati uz značajne mere opreza. Cilj ovog rada je da se predstave osnovni faktori i parametri koji utiču na reparatorni odgovor pulpe zuba kod obolelih od DM.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Diabetes mellitus and reparative response of dental pulp, Dijabetes melitus i reparativni odgovor zubne pulpe",
volume = "63",
number = "2",
pages = "85-90",
doi = "10.1515/sdj-2016-0009"
}

Ilić, J.. (2016). Diabetes mellitus and reparative response of dental pulp. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 63(2), 85-90.
https://doi.org/10.1515/sdj-2016-0009

Ilić J. Diabetes mellitus and reparative response of dental pulp. in Stomatološki glasnik Srbije. 2016;63(2):85-90.
doi:10.1515/sdj-2016-0009 .

Ilić, Jugoslav, "Diabetes mellitus and reparative response of dental pulp" in Stomatološki glasnik Srbije, 63, no. 2 (2016):85-90,
https://doi.org/10.1515/sdj-2016-0009 . .

TY  - THES
AU  - Milić, Marija
PY  - 2016
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=4677
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:14868/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48769807
UR  - http://nardus.mpn.gov.rs/123456789/7721
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/994
AB  - Introduction Patients with diabetes mellitus (DM) type 2 are at increased risk for performance of regional anaesthesia, due to macrovascular and microvascular complications of DM, including changes in small blood vessels of peripheral nerves (diabetic neuropathy). Results of experimental studies show that local anesthetics, even in clinically applicable concentrations, cause more pronounced damage to the nerves in the presence of DM, with clinically observable changes in parameters of regional anaesthesia. Neuropathic and microangiopathic changes caused by DM are also observed in the oral cavity tissues of DM patients. To date, there are no available data on efficacy and safety of intraoral local anaestehsia in patients with DM. Aims The aims of this doctoral dissertation were to test and compare efficacy and safety of intraoral local anaesthesia obtained with 2% lidocaine with clonidine (15 mcg / ml) (LC) and 2% lidocaine with epinephrine (1:80 000) (LE) in healthy and DM type 2 participants, by assessing the parameters of pulpal and soft tissues anaesthesia, pulpal blood flow, parameters of postoperative analgesia, presence of local side effects, as well as parameters of cardiovascular functions in the settings with and without oral surgical procedure. Material and method This prospective, double blind, randomized, controlled clinical study was performed in two parts. One part of the study included 36 healthy (ASA I) i 36 DM tip 2 participants (ASA II i ASA III), who received tested anaesthetic solutions (LC i LE) for maxillary infiltration anestehesia, in cross over-manner with washout period of 14 days, in the setting without oral surgical procedure. Tested parameters were: pulpal blood flow, parameters of pulpal anaesthesia and cardiovascular functions. Following part of the study involved 106 healthy (ASA I) i 127 DM tip 2 patients (ASA II i ASA III), divided into 8 groups according to health status, anaesthesia technique (infiltration, block) and tested anesthetic solution (LC, LE). In this study population the tested parameters were: soft tissue anaesthesia, postoperative analgesia, local side effects and cardiovascular parameters after infiltration and block application of either LC or LE, for extraction of single-rooted teeth in upper and lower jaw. Pulpal blood flow of maxillary central incisors was measured by laser Dopler flowmetry prior to, as well as 5, 10, 15, 30, 45 and 60 minutes after maxillary infiltration anaesthesia...
AB  - Uvod Pacijenti sa dijabetes melitusom (DM), zbog pratećih makrovaskularnih i mikrovaskularnih komplikacija osnovnog oboljenja, koje zahvataju i periferne nerve (dijabetična neuropatija), predstavljaju pacijente rizika za primenu regionalne anestezije. Pokazano je u eksperimentalnim uslovima da lokalni anestetici, u klinički primenljivim koncentracijama, izazivaju veća oštećenja na nervima izmenjenim dijabetičnom neuropatijom, što se u kliničkim uslovima manifestuje promenjenim parametrima regionalne anestezije. Promene koje DM izaziva na krvnim sudovima i nervima su zapažene i u tkivima i organima orofacijalne regije. Za sada nema podataka o parametrima i bezbednosti primene intraoralne lokalne anestezije u uslovima DM. Ciljevi Ovo istraživanje je imalo za ciljeve da ispita i uporedi efikasnost i bezbednost intraoralne lokalne anestezije postignute primenom 2% lidokaina sa klonidinom (15 mcg / ml) (LC) i 2% lidokaina sa epinefrinom (1:80 000) (LE) kod zdravih i ispitanika sa DM tip 2, praćenjem kliničkih parametara anestezije zubne pulpe i mekih tkiva, protoka krvi kroz zubnu pulpu, parametara postoperativne analgezije, učestalosti lokalnih neželjenih efekata, kao i parametara kardiovaskularne funkcije u uslovima sa i bez oralnohirurške intervencije. Materijal i metod Ova prospektivna, dvostruko slepa, randomizovana, kontrolisana klinička studija je izvedena u dva dela. U jednom delu studije učestvovalo je 36 zdravih (ASA I) i 36 ispitanika sa DM tip 2 (ASA II i ASA III), koji su u ukrštenom dizajnu istraživanja bez oralnohirurške intervencije, u razmaku od 14 dana dobili ispitivane anestetičke rastvore (LC i LE) za maksilarnu infiltracionu anesteziju, pri čemu su ispitivani protok krvi kroz zubnu pulpu maksilarnih centralnih sekutića, kao i parametri anestezije zubne pulpe i kardiovaskularne funkcije. U drugom delu studije učestvovalo je 106 zdravih (ASA I) i 127 ispitanika sa DM tip 2 (ASA II i ASA III), koji su prema zdravstvenom statusu, tehnici anestezije (infiltraciona, sprovodna) i vrsti anestetičkog rastvora (LC, LE) podeljeni u 8 grupa...
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - Efficacy and safety of lidocaine with clonidine for intraoral local anaesthesia in patients with diabetes mellitus type 2
T1  - Efikasnost i bezbednost intraoralne lokalne anestezije primenom lidokaina sa klonidinom kod pacijenata sa dijabetes melitusom tip 2
UR  - https://hdl.handle.net/21.15107/rcub_nardus_7721
ER  - 

@phdthesis{
author = "Milić, Marija",
year = "2016",
abstract = "Introduction Patients with diabetes mellitus (DM) type 2 are at increased risk for performance of regional anaesthesia, due to macrovascular and microvascular complications of DM, including changes in small blood vessels of peripheral nerves (diabetic neuropathy). Results of experimental studies show that local anesthetics, even in clinically applicable concentrations, cause more pronounced damage to the nerves in the presence of DM, with clinically observable changes in parameters of regional anaesthesia. Neuropathic and microangiopathic changes caused by DM are also observed in the oral cavity tissues of DM patients. To date, there are no available data on efficacy and safety of intraoral local anaestehsia in patients with DM. Aims The aims of this doctoral dissertation were to test and compare efficacy and safety of intraoral local anaesthesia obtained with 2% lidocaine with clonidine (15 mcg / ml) (LC) and 2% lidocaine with epinephrine (1:80 000) (LE) in healthy and DM type 2 participants, by assessing the parameters of pulpal and soft tissues anaesthesia, pulpal blood flow, parameters of postoperative analgesia, presence of local side effects, as well as parameters of cardiovascular functions in the settings with and without oral surgical procedure. Material and method This prospective, double blind, randomized, controlled clinical study was performed in two parts. One part of the study included 36 healthy (ASA I) i 36 DM tip 2 participants (ASA II i ASA III), who received tested anaesthetic solutions (LC i LE) for maxillary infiltration anestehesia, in cross over-manner with washout period of 14 days, in the setting without oral surgical procedure. Tested parameters were: pulpal blood flow, parameters of pulpal anaesthesia and cardiovascular functions. Following part of the study involved 106 healthy (ASA I) i 127 DM tip 2 patients (ASA II i ASA III), divided into 8 groups according to health status, anaesthesia technique (infiltration, block) and tested anesthetic solution (LC, LE). In this study population the tested parameters were: soft tissue anaesthesia, postoperative analgesia, local side effects and cardiovascular parameters after infiltration and block application of either LC or LE, for extraction of single-rooted teeth in upper and lower jaw. Pulpal blood flow of maxillary central incisors was measured by laser Dopler flowmetry prior to, as well as 5, 10, 15, 30, 45 and 60 minutes after maxillary infiltration anaesthesia..., Uvod Pacijenti sa dijabetes melitusom (DM), zbog pratećih makrovaskularnih i mikrovaskularnih komplikacija osnovnog oboljenja, koje zahvataju i periferne nerve (dijabetična neuropatija), predstavljaju pacijente rizika za primenu regionalne anestezije. Pokazano je u eksperimentalnim uslovima da lokalni anestetici, u klinički primenljivim koncentracijama, izazivaju veća oštećenja na nervima izmenjenim dijabetičnom neuropatijom, što se u kliničkim uslovima manifestuje promenjenim parametrima regionalne anestezije. Promene koje DM izaziva na krvnim sudovima i nervima su zapažene i u tkivima i organima orofacijalne regije. Za sada nema podataka o parametrima i bezbednosti primene intraoralne lokalne anestezije u uslovima DM. Ciljevi Ovo istraživanje je imalo za ciljeve da ispita i uporedi efikasnost i bezbednost intraoralne lokalne anestezije postignute primenom 2% lidokaina sa klonidinom (15 mcg / ml) (LC) i 2% lidokaina sa epinefrinom (1:80 000) (LE) kod zdravih i ispitanika sa DM tip 2, praćenjem kliničkih parametara anestezije zubne pulpe i mekih tkiva, protoka krvi kroz zubnu pulpu, parametara postoperativne analgezije, učestalosti lokalnih neželjenih efekata, kao i parametara kardiovaskularne funkcije u uslovima sa i bez oralnohirurške intervencije. Materijal i metod Ova prospektivna, dvostruko slepa, randomizovana, kontrolisana klinička studija je izvedena u dva dela. U jednom delu studije učestvovalo je 36 zdravih (ASA I) i 36 ispitanika sa DM tip 2 (ASA II i ASA III), koji su u ukrštenom dizajnu istraživanja bez oralnohirurške intervencije, u razmaku od 14 dana dobili ispitivane anestetičke rastvore (LC i LE) za maksilarnu infiltracionu anesteziju, pri čemu su ispitivani protok krvi kroz zubnu pulpu maksilarnih centralnih sekutića, kao i parametri anestezije zubne pulpe i kardiovaskularne funkcije. U drugom delu studije učestvovalo je 106 zdravih (ASA I) i 127 ispitanika sa DM tip 2 (ASA II i ASA III), koji su prema zdravstvenom statusu, tehnici anestezije (infiltraciona, sprovodna) i vrsti anestetičkog rastvora (LC, LE) podeljeni u 8 grupa...",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "Efficacy and safety of lidocaine with clonidine for intraoral local anaesthesia in patients with diabetes mellitus type 2, Efikasnost i bezbednost intraoralne lokalne anestezije primenom lidokaina sa klonidinom kod pacijenata sa dijabetes melitusom tip 2",
url = "https://hdl.handle.net/21.15107/rcub_nardus_7721"
}

Milić, M.. (2016). Efficacy and safety of lidocaine with clonidine for intraoral local anaesthesia in patients with diabetes mellitus type 2. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_7721

Milić M. Efficacy and safety of lidocaine with clonidine for intraoral local anaesthesia in patients with diabetes mellitus type 2. 2016;.
https://hdl.handle.net/21.15107/rcub_nardus_7721 .

Milić, Marija, "Efficacy and safety of lidocaine with clonidine for intraoral local anaesthesia in patients with diabetes mellitus type 2" (2016),
https://hdl.handle.net/21.15107/rcub_nardus_7721 .

TY  - JOUR
AU  - Milić, Marija
AU  - Brković, Božidar
AU  - Kršljak, Elena
AU  - Stojić, Dragica
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2179
AB  - The pulpal anesthetic and cardiovascular parameters obtained by 2 % lidocaine with epinephrine (LE; 1:80,000) or clonidine (LC; 15 mcg/ml) were studied in diabetes mellitus (DM) type 2 and healthy volunteers (72), after maxillary infiltration anesthesia. Onset and duration of pulpal anesthesia were measured by electric pulp tester; vasoconstrictive effect of used local anesthetic mixtures by laser Doppler flowmetry (LDF) through pulpal blood flow (PBF); systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR) were registered by electrocardiogram monitoring. Onset of pulpal anesthesia was shorter for LC than for LE in healthy, while it was not different in diabetic participants; duration of pulpal anesthesia was significantly longer in type 2 diabetic participants, regardless of used anesthetic mixture. Significant reduction of PBF with LE was observed during 45 min in healthy and 60 min in diabetic participants, while with LC such reduction was observed during 45 min in both groups. LE caused a significant increase of SBP in the 5th and 15th minutes in diabetic versus healthy participants, while LC decreased SBP from the 10th to 60th minutes in healthy versus diabetic participants. DM type 2 influences duration of maxillary infiltration anesthesia obtained with LE and LC, and systolic blood pressure during LE anesthesia. The obtained results provide elements for future protocols concerning intraoral local anesthesia in DM type 2 patients.
PB  - Springer Heidelberg, Heidelberg
T2  - Clinical Oral Investigations
T1  - Comparison of pulpal anesthesia and cardiovascular parameters with lidocaine with epinephrine and lidocaine with clonidine after maxillary infiltration in type 2 diabetic volunteers
VL  - 20
IS  - 6
SP  - 1283
EP  - 1293
DO  - 10.1007/s00784-015-1610-5
ER  - 

@article{
author = "Milić, Marija and Brković, Božidar and Kršljak, Elena and Stojić, Dragica",
year = "2016",
abstract = "The pulpal anesthetic and cardiovascular parameters obtained by 2 % lidocaine with epinephrine (LE; 1:80,000) or clonidine (LC; 15 mcg/ml) were studied in diabetes mellitus (DM) type 2 and healthy volunteers (72), after maxillary infiltration anesthesia. Onset and duration of pulpal anesthesia were measured by electric pulp tester; vasoconstrictive effect of used local anesthetic mixtures by laser Doppler flowmetry (LDF) through pulpal blood flow (PBF); systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR) were registered by electrocardiogram monitoring. Onset of pulpal anesthesia was shorter for LC than for LE in healthy, while it was not different in diabetic participants; duration of pulpal anesthesia was significantly longer in type 2 diabetic participants, regardless of used anesthetic mixture. Significant reduction of PBF with LE was observed during 45 min in healthy and 60 min in diabetic participants, while with LC such reduction was observed during 45 min in both groups. LE caused a significant increase of SBP in the 5th and 15th minutes in diabetic versus healthy participants, while LC decreased SBP from the 10th to 60th minutes in healthy versus diabetic participants. DM type 2 influences duration of maxillary infiltration anesthesia obtained with LE and LC, and systolic blood pressure during LE anesthesia. The obtained results provide elements for future protocols concerning intraoral local anesthesia in DM type 2 patients.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Clinical Oral Investigations",
title = "Comparison of pulpal anesthesia and cardiovascular parameters with lidocaine with epinephrine and lidocaine with clonidine after maxillary infiltration in type 2 diabetic volunteers",
volume = "20",
number = "6",
pages = "1283-1293",
doi = "10.1007/s00784-015-1610-5"
}

Milić, M., Brković, B., Kršljak, E.,& Stojić, D.. (2016). Comparison of pulpal anesthesia and cardiovascular parameters with lidocaine with epinephrine and lidocaine with clonidine after maxillary infiltration in type 2 diabetic volunteers. in Clinical Oral Investigations
Springer Heidelberg, Heidelberg., 20(6), 1283-1293.
https://doi.org/10.1007/s00784-015-1610-5

Milić M, Brković B, Kršljak E, Stojić D. Comparison of pulpal anesthesia and cardiovascular parameters with lidocaine with epinephrine and lidocaine with clonidine after maxillary infiltration in type 2 diabetic volunteers. in Clinical Oral Investigations. 2016;20(6):1283-1293.
doi:10.1007/s00784-015-1610-5 .

Milić, Marija, Brković, Božidar, Kršljak, Elena, Stojić, Dragica, "Comparison of pulpal anesthesia and cardiovascular parameters with lidocaine with epinephrine and lidocaine with clonidine after maxillary infiltration in type 2 diabetic volunteers" in Clinical Oral Investigations, 20, no. 6 (2016):1283-1293,
https://doi.org/10.1007/s00784-015-1610-5 . .

TY  - JOUR
AU  - Biočanin, Vladimir
AU  - Milić, Marija
AU  - Vučetić, Milan
AU  - Baćević, Miljana
AU  - Vasović, Dina
AU  - Živadinović, Milka
AU  - Ćetković, Dejan
AU  - Ćalasan, Dejan
AU  - Brković, Božidar
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2073
AB  - Introduction. The material used for root-end filling has to be biocompatible with adjacent periapical tissue and to stimulate its regenerative processes. Tricalcium silicate cement (TSC), as a new dental material, shows good sealing properties with dentin, high compression strengths and better marginal adaptation than commonly used root-end filling materials. Although optimal postoperative healing of periapical tissues is mainly influenced by characteristics of end-root material used, it could sometimes be affected by the influence of systemic diseases, such as diabetes mellitus (DM). Case report. We presented apical healing of the upper central incisor, retrofilled with TSC, in a diabetic patient (type 2 DM) with peripheral neuropathy. Standard root-end resection of upper central incisor was accompanied by retropreparation using ultrasonic retrotips to the depth of 3 mm and retrofilling with TSC. Postoperatively, the surgical wound healed uneventfully. However, the patient reported undefined dull pain in the operated area that could possibly be attributed to undiagnosed intraoral diabetic peripheral neuropathy, what was evaluated clinically. Conclusion. Although TSC presents a suitable material for apical root-end filling in the treatment of chronic periradicular lesions a possible presence of systemic diseases, like type 2 DM, has to be considered in the treatment outcome estimation.
AB  - Uvod. Materijal koji se koristi za retrogradnu opturaciju kanala korena trebalo bi da bude biokompatibilan sa okolnim periapeksnim tkivom i da stimuliše procese njegove regeneracije. Trikalcijum silikatni cement (TSC), kao novi dentalni materijal, pokazuje dobro zaptivanje, visoku kompresivnu snagu i bolju ivičnu adaptaciju u odnosu na standardno korišćene materijale za retroopturaciju. Iako postoperativno zarastanje periapikalnog tkiva najviše zavisi od karakteristika materijala za retroopturaciju, ponekad na uspeh zarastanja može uticati i prisustvo neke sistemske bolesti kao što je dijabetes melitus (DM). Prikaz bolesnika. Prikazali smo apikalno zarastanje u predelu gornjeg centralnog sekutića, nakon retroopturacije sa TSC, kod bolesnika sa DM tipa 2 i prisutnom perifernom neuropatijom. Standardna resekcija korena gornjeg centralnog sekutića bila je urađena retropreparacijom ultrazvučnim nastavcima do dubine od 3 mm i retrogradnom opturacijom sa TSC. Zarastanje postoperativne regije bilo je u fiziološkim granicama. Bolesnik se, međutim, žalio na nedefinisan, tup bol u predelu operisane regije koji je verovatno bio povezan sa nedijagnostikovanom intraoralnom dijabetičnom perifernom neuropatijom, što je potvrđeno kliničkim nalazom. Zaključak. Iako TSC predstavlja pogodan materijal za retrogradnu opturaciju kanala korena zuba u lečenju hroničnih periradikularnih lezija, u proceni ishoda lečenja treba imati u vidu i moguće prisustvo perifernih manfestacija sistemskih bolesti kao što je DM tipa 2.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Apical root-end filling with tricalcium silicate-based cement in a patient with diabetes mellitus: A case report
T1  - Punjenje kanala korena cementom na bazi trikalcijum-silikata kod bolesnika sa dijabetesom melitusom
VL  - 73
IS  - 12
SP  - 1173
EP  - 1177
DO  - 10.2298/VSP150606137B
ER  - 

@article{
author = "Biočanin, Vladimir and Milić, Marija and Vučetić, Milan and Baćević, Miljana and Vasović, Dina and Živadinović, Milka and Ćetković, Dejan and Ćalasan, Dejan and Brković, Božidar",
year = "2016",
abstract = "Introduction. The material used for root-end filling has to be biocompatible with adjacent periapical tissue and to stimulate its regenerative processes. Tricalcium silicate cement (TSC), as a new dental material, shows good sealing properties with dentin, high compression strengths and better marginal adaptation than commonly used root-end filling materials. Although optimal postoperative healing of periapical tissues is mainly influenced by characteristics of end-root material used, it could sometimes be affected by the influence of systemic diseases, such as diabetes mellitus (DM). Case report. We presented apical healing of the upper central incisor, retrofilled with TSC, in a diabetic patient (type 2 DM) with peripheral neuropathy. Standard root-end resection of upper central incisor was accompanied by retropreparation using ultrasonic retrotips to the depth of 3 mm and retrofilling with TSC. Postoperatively, the surgical wound healed uneventfully. However, the patient reported undefined dull pain in the operated area that could possibly be attributed to undiagnosed intraoral diabetic peripheral neuropathy, what was evaluated clinically. Conclusion. Although TSC presents a suitable material for apical root-end filling in the treatment of chronic periradicular lesions a possible presence of systemic diseases, like type 2 DM, has to be considered in the treatment outcome estimation., Uvod. Materijal koji se koristi za retrogradnu opturaciju kanala korena trebalo bi da bude biokompatibilan sa okolnim periapeksnim tkivom i da stimuliše procese njegove regeneracije. Trikalcijum silikatni cement (TSC), kao novi dentalni materijal, pokazuje dobro zaptivanje, visoku kompresivnu snagu i bolju ivičnu adaptaciju u odnosu na standardno korišćene materijale za retroopturaciju. Iako postoperativno zarastanje periapikalnog tkiva najviše zavisi od karakteristika materijala za retroopturaciju, ponekad na uspeh zarastanja može uticati i prisustvo neke sistemske bolesti kao što je dijabetes melitus (DM). Prikaz bolesnika. Prikazali smo apikalno zarastanje u predelu gornjeg centralnog sekutića, nakon retroopturacije sa TSC, kod bolesnika sa DM tipa 2 i prisutnom perifernom neuropatijom. Standardna resekcija korena gornjeg centralnog sekutića bila je urađena retropreparacijom ultrazvučnim nastavcima do dubine od 3 mm i retrogradnom opturacijom sa TSC. Zarastanje postoperativne regije bilo je u fiziološkim granicama. Bolesnik se, međutim, žalio na nedefinisan, tup bol u predelu operisane regije koji je verovatno bio povezan sa nedijagnostikovanom intraoralnom dijabetičnom perifernom neuropatijom, što je potvrđeno kliničkim nalazom. Zaključak. Iako TSC predstavlja pogodan materijal za retrogradnu opturaciju kanala korena zuba u lečenju hroničnih periradikularnih lezija, u proceni ishoda lečenja treba imati u vidu i moguće prisustvo perifernih manfestacija sistemskih bolesti kao što je DM tipa 2.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Apical root-end filling with tricalcium silicate-based cement in a patient with diabetes mellitus: A case report, Punjenje kanala korena cementom na bazi trikalcijum-silikata kod bolesnika sa dijabetesom melitusom",
volume = "73",
number = "12",
pages = "1173-1177",
doi = "10.2298/VSP150606137B"
}

Biočanin, V., Milić, M., Vučetić, M., Baćević, M., Vasović, D., Živadinović, M., Ćetković, D., Ćalasan, D.,& Brković, B.. (2016). Apical root-end filling with tricalcium silicate-based cement in a patient with diabetes mellitus: A case report. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 73(12), 1173-1177.
https://doi.org/10.2298/VSP150606137B

Biočanin V, Milić M, Vučetić M, Baćević M, Vasović D, Živadinović M, Ćetković D, Ćalasan D, Brković B. Apical root-end filling with tricalcium silicate-based cement in a patient with diabetes mellitus: A case report. in Vojnosanitetski pregled. 2016;73(12):1173-1177.
doi:10.2298/VSP150606137B .

Biočanin, Vladimir, Milić, Marija, Vučetić, Milan, Baćević, Miljana, Vasović, Dina, Živadinović, Milka, Ćetković, Dejan, Ćalasan, Dejan, Brković, Božidar, "Apical root-end filling with tricalcium silicate-based cement in a patient with diabetes mellitus: A case report" in Vojnosanitetski pregled, 73, no. 12 (2016):1173-1177,
https://doi.org/10.2298/VSP150606137B . .