TY  - JOUR
AU  - Oprić, D.
AU  - Stankovich, A.D.
AU  - Nenadović, A.
AU  - Kovačević, S.
AU  - Obradović, D.D.
AU  - de Luka, Silvio R.
AU  - Nešović-Ostojić, J.
AU  - Milašin, Jelena
AU  - Ilić, A.Ž.
AU  - Trbovich, Alexander M.
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2506
AB  - Objective: Inflammation is a biological response of tissue to harmful stimuli. A high-fat diet was linked to low-grade chronic liver inflammation, which can further lead to more severe health conditions. It is crucial to assess the intensity of inflammation and structural tissue changes to reduce the subjective judgment by the examiner. We propose fractal-based methods for early-stage low-degree liver inflammation grading. Methods: We have randomly divided 40 C57BL/6 female mice into four groups (control, linseed oil, palm oil, sunflower oil). After 100 days, animals were euthanized, and liver tissue collected for analyses. We performed calculations of fractal dimension, fractal lacunarity, multifractal spectra, local fractal dimension, and particle metrics, applicable to tissue segmentation and grading. Results: Pathohistological analysis of some liver tissue showed a low-grade inflammatory infiltrate around the portal vein of experimental groups subjected to different high-fat diets. Differences in fractal dimension and lacunarity of the inflamed tissue were, in most cases, statistically significant between the high-fat diet groups. Both the observed intensity and area of inflammation were lowest for the sunflower oil. The results of standard fractal analysis, local fractal analysis, and particle analysis were in an excellent agreement. Conclusions: This study demonstrated the efficiency of the fractal analysis based tools in the quantification of complexity and early-stage structural changes in inflamed liver tissue, which could potentially be used in the diagnostic workup of inflammation in the liver. The presented methods could be implemented within a wider scope computer-aided diagnostics system in a very straightforward manner.
PB  - Elsevier Ltd
T2  - Biomedical Signal Processing & Control
T1  - Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils
VL  - 61
DO  - 10.1016/j.bspc.2020.101959
ER  - 

@article{
author = "Oprić, D. and Stankovich, A.D. and Nenadović, A. and Kovačević, S. and Obradović, D.D. and de Luka, Silvio R. and Nešović-Ostojić, J. and Milašin, Jelena and Ilić, A.Ž. and Trbovich, Alexander M.",
year = "2020",
abstract = "Objective: Inflammation is a biological response of tissue to harmful stimuli. A high-fat diet was linked to low-grade chronic liver inflammation, which can further lead to more severe health conditions. It is crucial to assess the intensity of inflammation and structural tissue changes to reduce the subjective judgment by the examiner. We propose fractal-based methods for early-stage low-degree liver inflammation grading. Methods: We have randomly divided 40 C57BL/6 female mice into four groups (control, linseed oil, palm oil, sunflower oil). After 100 days, animals were euthanized, and liver tissue collected for analyses. We performed calculations of fractal dimension, fractal lacunarity, multifractal spectra, local fractal dimension, and particle metrics, applicable to tissue segmentation and grading. Results: Pathohistological analysis of some liver tissue showed a low-grade inflammatory infiltrate around the portal vein of experimental groups subjected to different high-fat diets. Differences in fractal dimension and lacunarity of the inflamed tissue were, in most cases, statistically significant between the high-fat diet groups. Both the observed intensity and area of inflammation were lowest for the sunflower oil. The results of standard fractal analysis, local fractal analysis, and particle analysis were in an excellent agreement. Conclusions: This study demonstrated the efficiency of the fractal analysis based tools in the quantification of complexity and early-stage structural changes in inflamed liver tissue, which could potentially be used in the diagnostic workup of inflammation in the liver. The presented methods could be implemented within a wider scope computer-aided diagnostics system in a very straightforward manner.",
publisher = "Elsevier Ltd",
journal = "Biomedical Signal Processing & Control",
title = "Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils",
volume = "61",
doi = "10.1016/j.bspc.2020.101959"
}

Oprić, D., Stankovich, A.D., Nenadović, A., Kovačević, S., Obradović, D.D., de Luka, S. R., Nešović-Ostojić, J., Milašin, J., Ilić, A.Ž.,& Trbovich, A. M.. (2020). Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils. in Biomedical Signal Processing & Control
Elsevier Ltd., 61.
https://doi.org/10.1016/j.bspc.2020.101959

Oprić D, Stankovich A, Nenadović A, Kovačević S, Obradović D, de Luka SR, Nešović-Ostojić J, Milašin J, Ilić A, Trbovich AM. Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils. in Biomedical Signal Processing & Control. 2020;61.
doi:10.1016/j.bspc.2020.101959 .

Oprić, D., Stankovich, A.D., Nenadović, A., Kovačević, S., Obradović, D.D., de Luka, Silvio R., Nešović-Ostojić, J., Milašin, Jelena, Ilić, A.Ž., Trbovich, Alexander M., "Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils" in Biomedical Signal Processing & Control, 61 (2020),
https://doi.org/10.1016/j.bspc.2020.101959 . .

TY  - JOUR
AU  - Popović, Branka
AU  - Velimirović, Milica
AU  - Stojković, Tihomir
AU  - Brajović, Gavrilo
AU  - de Luka, Silvio R.
AU  - Milovanović, Ivan
AU  - Stefanović, Srđan
AU  - Nikolić, Dragica
AU  - Ristic-Đurović, Jasna L.
AU  - Petronijević, Nataša
AU  - Trbovich, Alexander M.
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2323
AB  - Ageing affects various physiological and metabolic processes in a body and a progressive accumulation of oxidative damage stands out as often used explanation. One of the most powerful scavenger of reactive oxygen species (ROS) in all organs is melatonin. A majority of melatonin supplied to the body via blood originates from the pineal gland. However, we have been interested in a locally produced melatonin. We have used 2.5- and 36-months-old Wistar rats. Tissues were collected and gene expression of AA-NAT and ASMT, the two key enzymes in a synthesis of melatonin, was determined in brain, liver, kidney, heart, skin, and intestine. Since melatonin can influence antioxidant enzymes, the activity of superoxide dismutase (SOD) and catalase (CAT), and the level of GSH were measured in liver. In addition, Copper (Cu), Zinc (Zn), and Manganese (Mn) were also determined in liver since these microelements might affect the activity of antioxidant enzymes. The expression of AA-NAT and ASMT was increased in liver and skin of old animals. A positive correlation in AA-NAT and ASMT expression was observed in liver, intestine and kidney. Moreover, the activity of CAT enzyme in liver was increased while SOD activity was decreased. SOD and CAT were probably affected by the observed decreased amount of Cu, Zn, and Mn in liver of old animals. In our model, extrapineal melatonin pathway in ageing consisted of complex interplay of locally produced melatonin, activities of SOD and CAT, and adequate presence of Cu, Zn and Mn microelements in order to defend organs against oxidative damage.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - The influence of ageing on the extrapineal melatonin synthetic pathway
VL  - 110
SP  - 151
EP  - 157
DO  - 10.1016/j.exger.2018.06.010
ER  - 

@article{
author = "Popović, Branka and Velimirović, Milica and Stojković, Tihomir and Brajović, Gavrilo and de Luka, Silvio R. and Milovanović, Ivan and Stefanović, Srđan and Nikolić, Dragica and Ristic-Đurović, Jasna L. and Petronijević, Nataša and Trbovich, Alexander M.",
year = "2018",
abstract = "Ageing affects various physiological and metabolic processes in a body and a progressive accumulation of oxidative damage stands out as often used explanation. One of the most powerful scavenger of reactive oxygen species (ROS) in all organs is melatonin. A majority of melatonin supplied to the body via blood originates from the pineal gland. However, we have been interested in a locally produced melatonin. We have used 2.5- and 36-months-old Wistar rats. Tissues were collected and gene expression of AA-NAT and ASMT, the two key enzymes in a synthesis of melatonin, was determined in brain, liver, kidney, heart, skin, and intestine. Since melatonin can influence antioxidant enzymes, the activity of superoxide dismutase (SOD) and catalase (CAT), and the level of GSH were measured in liver. In addition, Copper (Cu), Zinc (Zn), and Manganese (Mn) were also determined in liver since these microelements might affect the activity of antioxidant enzymes. The expression of AA-NAT and ASMT was increased in liver and skin of old animals. A positive correlation in AA-NAT and ASMT expression was observed in liver, intestine and kidney. Moreover, the activity of CAT enzyme in liver was increased while SOD activity was decreased. SOD and CAT were probably affected by the observed decreased amount of Cu, Zn, and Mn in liver of old animals. In our model, extrapineal melatonin pathway in ageing consisted of complex interplay of locally produced melatonin, activities of SOD and CAT, and adequate presence of Cu, Zn and Mn microelements in order to defend organs against oxidative damage.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "The influence of ageing on the extrapineal melatonin synthetic pathway",
volume = "110",
pages = "151-157",
doi = "10.1016/j.exger.2018.06.010"
}

Popović, B., Velimirović, M., Stojković, T., Brajović, G., de Luka, S. R., Milovanović, I., Stefanović, S., Nikolić, D., Ristic-Đurović, J. L., Petronijević, N.,& Trbovich, A. M.. (2018). The influence of ageing on the extrapineal melatonin synthetic pathway. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 110, 151-157.
https://doi.org/10.1016/j.exger.2018.06.010

Popović B, Velimirović M, Stojković T, Brajović G, de Luka SR, Milovanović I, Stefanović S, Nikolić D, Ristic-Đurović JL, Petronijević N, Trbovich AM. The influence of ageing on the extrapineal melatonin synthetic pathway. in Experimental Gerontology. 2018;110:151-157.
doi:10.1016/j.exger.2018.06.010 .

Popović, Branka, Velimirović, Milica, Stojković, Tihomir, Brajović, Gavrilo, de Luka, Silvio R., Milovanović, Ivan, Stefanović, Srđan, Nikolić, Dragica, Ristic-Đurović, Jasna L., Petronijević, Nataša, Trbovich, Alexander M., "The influence of ageing on the extrapineal melatonin synthetic pathway" in Experimental Gerontology, 110 (2018):151-157,
https://doi.org/10.1016/j.exger.2018.06.010 . .