TY  - JOUR
AU  - Krunić, Jelena
AU  - Stojanović, Nikola
AU  - Đukić, Ljiljana
AU  - Roganović, Jelena
AU  - Popović, Branka
AU  - Simić, Ivana
AU  - Stojić, Dragica
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2465
AB  - ObjectivesTo evaluate local effect of gaseous ozone on bacteria in deep carious lesions after incomplete caries removal, using chlorhexidine as control, and to investigate its effect on pulp vascular endothelial growth factor (VEGF), neuronal nitric oxide synthase (nNOS), and superoxide dismutase (SOD).Materials and methodsAntibacterial effect was evaluated in 48 teeth with diagnosed deep carious lesion. After incomplete caries removal, teeth were randomly allocated into two groups regarding the cavity disinfectant used: ozone (open system) or 2% chlorhexidine. Dentin samples were analyzed for the presence of total bacteria and Lactobacillus spp. by real-time quantitative polymerase chain reaction. For evaluation of ozone effect on dental pulp, 38 intact permanent teeth indicated for pulp removal/tooth extraction were included. After cavity preparation, teeth were randomly allocated into two groups: ozone group and control group. VEGF/nNOS level and SOD activity in dental pulp were determined by enzyme-linked immunosorbent assay and spectrophotometric method, respectively.ResultsOzone application decreased number of total bacteria (p=0.001) and Lactobacillus spp. (p lt 0.001), similarly to chlorhexidine. The VEGF (p lt 0.001) and nNOS (p=0.012) levels in dental pulp after ozone application were higher, while SOD activity was lower (p=0.001) comparing to those in control pulp.ConclusionsAntibacterial effect of ozone on residual bacteria after incomplete caries removal was similar to that of 2% chlorhexidine. Effect of ozone on pulp VEGF, nNOS, and SOD indicated its biocompatibility.Clinical relevanceOzone appears as effective and biocompatible cavity disinfectant in treatment of deep carious lesions by incomplete caries removal technique.
PB  - Springer Heidelberg, Heidelberg
T2  - Clinical Oral Investigations
T1  - Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal
VL  - 23
IS  - 2
SP  - 785
EP  - 792
DO  - 10.1007/s00784-018-2495-x
ER  - 

@article{
author = "Krunić, Jelena and Stojanović, Nikola and Đukić, Ljiljana and Roganović, Jelena and Popović, Branka and Simić, Ivana and Stojić, Dragica",
year = "2019",
abstract = "ObjectivesTo evaluate local effect of gaseous ozone on bacteria in deep carious lesions after incomplete caries removal, using chlorhexidine as control, and to investigate its effect on pulp vascular endothelial growth factor (VEGF), neuronal nitric oxide synthase (nNOS), and superoxide dismutase (SOD).Materials and methodsAntibacterial effect was evaluated in 48 teeth with diagnosed deep carious lesion. After incomplete caries removal, teeth were randomly allocated into two groups regarding the cavity disinfectant used: ozone (open system) or 2% chlorhexidine. Dentin samples were analyzed for the presence of total bacteria and Lactobacillus spp. by real-time quantitative polymerase chain reaction. For evaluation of ozone effect on dental pulp, 38 intact permanent teeth indicated for pulp removal/tooth extraction were included. After cavity preparation, teeth were randomly allocated into two groups: ozone group and control group. VEGF/nNOS level and SOD activity in dental pulp were determined by enzyme-linked immunosorbent assay and spectrophotometric method, respectively.ResultsOzone application decreased number of total bacteria (p=0.001) and Lactobacillus spp. (p lt 0.001), similarly to chlorhexidine. The VEGF (p lt 0.001) and nNOS (p=0.012) levels in dental pulp after ozone application were higher, while SOD activity was lower (p=0.001) comparing to those in control pulp.ConclusionsAntibacterial effect of ozone on residual bacteria after incomplete caries removal was similar to that of 2% chlorhexidine. Effect of ozone on pulp VEGF, nNOS, and SOD indicated its biocompatibility.Clinical relevanceOzone appears as effective and biocompatible cavity disinfectant in treatment of deep carious lesions by incomplete caries removal technique.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Clinical Oral Investigations",
title = "Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal",
volume = "23",
number = "2",
pages = "785-792",
doi = "10.1007/s00784-018-2495-x"
}

Krunić, J., Stojanović, N., Đukić, L., Roganović, J., Popović, B., Simić, I.,& Stojić, D.. (2019). Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal. in Clinical Oral Investigations
Springer Heidelberg, Heidelberg., 23(2), 785-792.
https://doi.org/10.1007/s00784-018-2495-x

Krunić J, Stojanović N, Đukić L, Roganović J, Popović B, Simić I, Stojić D. Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal. in Clinical Oral Investigations. 2019;23(2):785-792.
doi:10.1007/s00784-018-2495-x .

Krunić, Jelena, Stojanović, Nikola, Đukić, Ljiljana, Roganović, Jelena, Popović, Branka, Simić, Ivana, Stojić, Dragica, "Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal" in Clinical Oral Investigations, 23, no. 2 (2019):785-792,
https://doi.org/10.1007/s00784-018-2495-x . .