TY  - JOUR
AU  - Radunović, Milena
AU  - De Colli, Marianna
AU  - De Marco, Patrizia
AU  - Di Nisio, Chiara
AU  - Fontana, Antonella
AU  - Piattelli, Adriano
AU  - Cataldi, Amelia
AU  - Zara, Susi
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2186
AB  - Collagen membranes are used in oral surgery for bone defects treatment acting as a barrier that does not allow the invasion of soft tissue into the growing bone. To improve biocompatibility collagen membranes were coated with graphene oxide (GO), a graphene derivative. The aim of this study was to investigate the biocompatibility of GO coated collagen membranes on human dental pulp stem cells (DPSCs) focusing on biomaterial cytotoxicity, ability to promote DPSCs differentiation process and to control inflammation event induction. DPSCs were cultured on uncoated membranes and on both 2 and 10 mu g mL(-1) GO coated membranes up to 28 days. Alamar blue and LDH cytotocicity assay, PGE2 ELISA assay, real time RT-PCR for RUNX2, BMP2, SP7, TNF alpha and COX2 genes expression were performed. Proliferation is higher on GO coated membranes at days 14 and 28. LDH assay evidences no cytotoxicity. BMP2 and RUNX2 expression is higher on coated membranes, BMP2 at early and RUNX2 and SP7 at late experimental times. PGE2 levels are lower on GO coated membranes at days 14 and 28, both TNF alpha and COX2 expression is significantly decreased when GO is applied. GO coated membranes are not toxic for DPSCs, induce a faster DPSCs differentiation into odontoblasts/osteoblasts and may represent good alternative to conventional membranes thus ensuring more efficient bone formation and improving the clinical performance.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence
VL  - 105
IS  - 8
SP  - 2312
EP  - 2320
DO  - 10.1002/jbm.a.36085
ER  - 

@article{
author = "Radunović, Milena and De Colli, Marianna and De Marco, Patrizia and Di Nisio, Chiara and Fontana, Antonella and Piattelli, Adriano and Cataldi, Amelia and Zara, Susi",
year = "2017",
abstract = "Collagen membranes are used in oral surgery for bone defects treatment acting as a barrier that does not allow the invasion of soft tissue into the growing bone. To improve biocompatibility collagen membranes were coated with graphene oxide (GO), a graphene derivative. The aim of this study was to investigate the biocompatibility of GO coated collagen membranes on human dental pulp stem cells (DPSCs) focusing on biomaterial cytotoxicity, ability to promote DPSCs differentiation process and to control inflammation event induction. DPSCs were cultured on uncoated membranes and on both 2 and 10 mu g mL(-1) GO coated membranes up to 28 days. Alamar blue and LDH cytotocicity assay, PGE2 ELISA assay, real time RT-PCR for RUNX2, BMP2, SP7, TNF alpha and COX2 genes expression were performed. Proliferation is higher on GO coated membranes at days 14 and 28. LDH assay evidences no cytotoxicity. BMP2 and RUNX2 expression is higher on coated membranes, BMP2 at early and RUNX2 and SP7 at late experimental times. PGE2 levels are lower on GO coated membranes at days 14 and 28, both TNF alpha and COX2 expression is significantly decreased when GO is applied. GO coated membranes are not toxic for DPSCs, induce a faster DPSCs differentiation into odontoblasts/osteoblasts and may represent good alternative to conventional membranes thus ensuring more efficient bone formation and improving the clinical performance.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence",
volume = "105",
number = "8",
pages = "2312-2320",
doi = "10.1002/jbm.a.36085"
}

Radunović, M., De Colli, M., De Marco, P., Di Nisio, C., Fontana, A., Piattelli, A., Cataldi, A.,& Zara, S.. (2017). Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 105(8), 2312-2320.
https://doi.org/10.1002/jbm.a.36085

Radunović M, De Colli M, De Marco P, Di Nisio C, Fontana A, Piattelli A, Cataldi A, Zara S. Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence. in Journal of Biomedical Materials Research Part A. 2017;105(8):2312-2320.
doi:10.1002/jbm.a.36085 .

Radunović, Milena, De Colli, Marianna, De Marco, Patrizia, Di Nisio, Chiara, Fontana, Antonella, Piattelli, Adriano, Cataldi, Amelia, Zara, Susi, "Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence" in Journal of Biomedical Materials Research Part A, 105, no. 8 (2017):2312-2320,
https://doi.org/10.1002/jbm.a.36085 . .