TY  - JOUR
AU  - Čolić, Miodrag
AU  - Tomić, Sergej
AU  - Rudolf, Rebeka
AU  - Marković, Evgenija
AU  - Šćepan, Ivana
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2090
AB  - Superelastic (SE) and thermo-activated (TA) nickel-titanium (NiTi) archwires are used in everyday orthodontic practice, based on their acceptable biocompatibility and well-defined shape memory properties. However, the differences in their surface microstructure and cytotoxicity have not been clearly defined, and the standard cytotoxicity tests are too robust to detect small differences in the cytotoxicity of these alloys, all of which can lead to unexpected adverse reactions in some patients. Therefore, we tested the hypothesis that the differences in manufacture and microstructure of commercially available SE and TA archwires may influence their biocompatibility. The archwires were studied as-received and after conditioning for 24 h or 35 days in a cell culture medium under static conditions. All of the tested archwires, including their conditioned medium (CM), were non-cytotoxic for L929 cells, but Rematitan SE (both as received and conditioned) induced the apoptosis of rat thymocytes in a direct contact. In contrast, TruFlex SE and Equire TA increased the proliferation of thymocytes. The cytotoxic effect of Rematitan SE correlated with the higher release of Ni ions in CM, higher concentration of surface Ni and an increased oxygen layer thickness after the conditioning. In conclusion, the apoptosis assay on rat thymocytes, in contrast to the less sensitive standard assay on L929 cells, revealed that Rematitan SE was less cytocompatible compared to other archwires and the effect was most probably associated with a higher exposition of the cells to Ni on the surface of the archwire, due to the formation of unstable oxide layer.
PB  - Springer, Dordrecht
T2  - Journal of Materials Science-Materials in Medicine
T1  - Differences in cytocompatibility, dynamics of the oxide layers' formation, and nickel release between superelastic and thermo-activated nickel-titanium archwires
VL  - 27
IS  - 8
DO  - 10.1007/s10856-016-5742-1
ER  - 

@article{
author = "Čolić, Miodrag and Tomić, Sergej and Rudolf, Rebeka and Marković, Evgenija and Šćepan, Ivana",
year = "2016",
abstract = "Superelastic (SE) and thermo-activated (TA) nickel-titanium (NiTi) archwires are used in everyday orthodontic practice, based on their acceptable biocompatibility and well-defined shape memory properties. However, the differences in their surface microstructure and cytotoxicity have not been clearly defined, and the standard cytotoxicity tests are too robust to detect small differences in the cytotoxicity of these alloys, all of which can lead to unexpected adverse reactions in some patients. Therefore, we tested the hypothesis that the differences in manufacture and microstructure of commercially available SE and TA archwires may influence their biocompatibility. The archwires were studied as-received and after conditioning for 24 h or 35 days in a cell culture medium under static conditions. All of the tested archwires, including their conditioned medium (CM), were non-cytotoxic for L929 cells, but Rematitan SE (both as received and conditioned) induced the apoptosis of rat thymocytes in a direct contact. In contrast, TruFlex SE and Equire TA increased the proliferation of thymocytes. The cytotoxic effect of Rematitan SE correlated with the higher release of Ni ions in CM, higher concentration of surface Ni and an increased oxygen layer thickness after the conditioning. In conclusion, the apoptosis assay on rat thymocytes, in contrast to the less sensitive standard assay on L929 cells, revealed that Rematitan SE was less cytocompatible compared to other archwires and the effect was most probably associated with a higher exposition of the cells to Ni on the surface of the archwire, due to the formation of unstable oxide layer.",
publisher = "Springer, Dordrecht",
journal = "Journal of Materials Science-Materials in Medicine",
title = "Differences in cytocompatibility, dynamics of the oxide layers' formation, and nickel release between superelastic and thermo-activated nickel-titanium archwires",
volume = "27",
number = "8",
doi = "10.1007/s10856-016-5742-1"
}

Čolić, M., Tomić, S., Rudolf, R., Marković, E.,& Šćepan, I.. (2016). Differences in cytocompatibility, dynamics of the oxide layers' formation, and nickel release between superelastic and thermo-activated nickel-titanium archwires. in Journal of Materials Science-Materials in Medicine
Springer, Dordrecht., 27(8).
https://doi.org/10.1007/s10856-016-5742-1

Čolić M, Tomić S, Rudolf R, Marković E, Šćepan I. Differences in cytocompatibility, dynamics of the oxide layers' formation, and nickel release between superelastic and thermo-activated nickel-titanium archwires. in Journal of Materials Science-Materials in Medicine. 2016;27(8).
doi:10.1007/s10856-016-5742-1 .

Čolić, Miodrag, Tomić, Sergej, Rudolf, Rebeka, Marković, Evgenija, Šćepan, Ivana, "Differences in cytocompatibility, dynamics of the oxide layers' formation, and nickel release between superelastic and thermo-activated nickel-titanium archwires" in Journal of Materials Science-Materials in Medicine, 27, no. 8 (2016),
https://doi.org/10.1007/s10856-016-5742-1 . .