TY  - JOUR
AU  - Brajović, Gavrilo
AU  - Stefanović, Gordana
AU  - Ilić, Vesna
AU  - Petrović, Sonja
AU  - Stefanović, Neda
AU  - Nikolić-Jakoba, Nataša
AU  - Milošević-Jovčić, Nadežda
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1563
AB  - Background: Fibronectin (FN) can bind to immunoglobulins (Ig), influencing both the normal clearance and abnormal deposition of circulating immune complexes. This study focuses on the possible interaction between FN and IgG present in gingival crevicular fluid (GCF) of periodontitis patients and periodontally healthy controls, with the aim to determine whether such interaction may be connected with the glycosylation profile of IgG and, thus, consequentional in accumulation of proinflammatory IgG in periodontal pockets. Methods: The study included 30 patients with initial or advanced periodontitis, and 13 periodontally healthy subjects. GCF IgG was purified and analyzed for the presence of FN and its fragments and for galactose expression. Results: IgG isolated from GCF contained FN, which was bound to the IgG heavy chains. IgG from GCF of advanced periodontitis patients contained high levels of hypogalactosylated IgG, and bound more FN than IgG from GCF of the other two groups, which contained low levels of this glycoform. FN was in a degraded form in GCF from all studied patients, and a fragment of 48- to 53-kDa molecular mass seemed to be the sole one linked to IgG. Conclusions: IgG and the FN fragment of 48 to 53 kDa in GCF of periodontitis patients and periodontally healthy subjects are physically connected. This fragment was linked to the heavy chains of IgG and the reaction seemed to be particularly intensive with IgG from advanced periodontitis, which contained significantly less galactose in its sugar chains. J Periodontol 2010;81:1472-1480.
PB  - Wiley, Hoboken
T2  - Journal of Periodontology
T1  - Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis
VL  - 81
IS  - 10
SP  - 1472
EP  - 1480
DO  - 10.1902/jop.2010.100053
ER  - 

@article{
author = "Brajović, Gavrilo and Stefanović, Gordana and Ilić, Vesna and Petrović, Sonja and Stefanović, Neda and Nikolić-Jakoba, Nataša and Milošević-Jovčić, Nadežda",
year = "2010",
abstract = "Background: Fibronectin (FN) can bind to immunoglobulins (Ig), influencing both the normal clearance and abnormal deposition of circulating immune complexes. This study focuses on the possible interaction between FN and IgG present in gingival crevicular fluid (GCF) of periodontitis patients and periodontally healthy controls, with the aim to determine whether such interaction may be connected with the glycosylation profile of IgG and, thus, consequentional in accumulation of proinflammatory IgG in periodontal pockets. Methods: The study included 30 patients with initial or advanced periodontitis, and 13 periodontally healthy subjects. GCF IgG was purified and analyzed for the presence of FN and its fragments and for galactose expression. Results: IgG isolated from GCF contained FN, which was bound to the IgG heavy chains. IgG from GCF of advanced periodontitis patients contained high levels of hypogalactosylated IgG, and bound more FN than IgG from GCF of the other two groups, which contained low levels of this glycoform. FN was in a degraded form in GCF from all studied patients, and a fragment of 48- to 53-kDa molecular mass seemed to be the sole one linked to IgG. Conclusions: IgG and the FN fragment of 48 to 53 kDa in GCF of periodontitis patients and periodontally healthy subjects are physically connected. This fragment was linked to the heavy chains of IgG and the reaction seemed to be particularly intensive with IgG from advanced periodontitis, which contained significantly less galactose in its sugar chains. J Periodontol 2010;81:1472-1480.",
publisher = "Wiley, Hoboken",
journal = "Journal of Periodontology",
title = "Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis",
volume = "81",
number = "10",
pages = "1472-1480",
doi = "10.1902/jop.2010.100053"
}

Brajović, G., Stefanović, G., Ilić, V., Petrović, S., Stefanović, N., Nikolić-Jakoba, N.,& Milošević-Jovčić, N.. (2010). Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis. in Journal of Periodontology
Wiley, Hoboken., 81(10), 1472-1480.
https://doi.org/10.1902/jop.2010.100053

Brajović G, Stefanović G, Ilić V, Petrović S, Stefanović N, Nikolić-Jakoba N, Milošević-Jovčić N. Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis. in Journal of Periodontology. 2010;81(10):1472-1480.
doi:10.1902/jop.2010.100053 .

Brajović, Gavrilo, Stefanović, Gordana, Ilić, Vesna, Petrović, Sonja, Stefanović, Neda, Nikolić-Jakoba, Nataša, Milošević-Jovčić, Nadežda, "Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis" in Journal of Periodontology, 81, no. 10 (2010):1472-1480,
https://doi.org/10.1902/jop.2010.100053 . .