Hypothalamic and medullary functional genomics in stress-induced hypertension

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info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41013/RS//

Hypothalamic and medullary functional genomics in stress-induced hypertension (en)
Функционална геномика хипоталамуса и медуле у хипертензији индукованој хроничним стресом (sr)
Funkcionalna genomika hipotalamusa i medule u hipertenziji indukovanoj hroničnim stresom (sr_RS)
Authors

Publications

Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils

Oprić, D.; Stankovich, A.D.; Nenadović, A.; Kovačević, S.; Obradović, D.D.; de Luka, Silvio R.; Nešović-Ostojić, J.; Milašin, Jelena; Ilić, A.Ž.; Trbovich, Alexander M.

(Elsevier Ltd, 2020)

TY  - JOUR
AU  - Oprić, D.
AU  - Stankovich, A.D.
AU  - Nenadović, A.
AU  - Kovačević, S.
AU  - Obradović, D.D.
AU  - de Luka, Silvio R.
AU  - Nešović-Ostojić, J.
AU  - Milašin, Jelena
AU  - Ilić, A.Ž.
AU  - Trbovich, Alexander M.
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2506
AB  - Objective: Inflammation is a biological response of tissue to harmful stimuli. A high-fat diet was linked to low-grade chronic liver inflammation, which can further lead to more severe health conditions. It is crucial to assess the intensity of inflammation and structural tissue changes to reduce the subjective judgment by the examiner. We propose fractal-based methods for early-stage low-degree liver inflammation grading. Methods: We have randomly divided 40 C57BL/6 female mice into four groups (control, linseed oil, palm oil, sunflower oil). After 100 days, animals were euthanized, and liver tissue collected for analyses. We performed calculations of fractal dimension, fractal lacunarity, multifractal spectra, local fractal dimension, and particle metrics, applicable to tissue segmentation and grading. Results: Pathohistological analysis of some liver tissue showed a low-grade inflammatory infiltrate around the portal vein of experimental groups subjected to different high-fat diets. Differences in fractal dimension and lacunarity of the inflamed tissue were, in most cases, statistically significant between the high-fat diet groups. Both the observed intensity and area of inflammation were lowest for the sunflower oil. The results of standard fractal analysis, local fractal analysis, and particle analysis were in an excellent agreement. Conclusions: This study demonstrated the efficiency of the fractal analysis based tools in the quantification of complexity and early-stage structural changes in inflamed liver tissue, which could potentially be used in the diagnostic workup of inflammation in the liver. The presented methods could be implemented within a wider scope computer-aided diagnostics system in a very straightforward manner.
PB  - Elsevier Ltd
T2  - Biomedical Signal Processing & Control
T1  - Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils
VL  - 61
DO  - 10.1016/j.bspc.2020.101959
ER  - 
@article{
author = "Oprić, D. and Stankovich, A.D. and Nenadović, A. and Kovačević, S. and Obradović, D.D. and de Luka, Silvio R. and Nešović-Ostojić, J. and Milašin, Jelena and Ilić, A.Ž. and Trbovich, Alexander M.",
year = "2020",
abstract = "Objective: Inflammation is a biological response of tissue to harmful stimuli. A high-fat diet was linked to low-grade chronic liver inflammation, which can further lead to more severe health conditions. It is crucial to assess the intensity of inflammation and structural tissue changes to reduce the subjective judgment by the examiner. We propose fractal-based methods for early-stage low-degree liver inflammation grading. Methods: We have randomly divided 40 C57BL/6 female mice into four groups (control, linseed oil, palm oil, sunflower oil). After 100 days, animals were euthanized, and liver tissue collected for analyses. We performed calculations of fractal dimension, fractal lacunarity, multifractal spectra, local fractal dimension, and particle metrics, applicable to tissue segmentation and grading. Results: Pathohistological analysis of some liver tissue showed a low-grade inflammatory infiltrate around the portal vein of experimental groups subjected to different high-fat diets. Differences in fractal dimension and lacunarity of the inflamed tissue were, in most cases, statistically significant between the high-fat diet groups. Both the observed intensity and area of inflammation were lowest for the sunflower oil. The results of standard fractal analysis, local fractal analysis, and particle analysis were in an excellent agreement. Conclusions: This study demonstrated the efficiency of the fractal analysis based tools in the quantification of complexity and early-stage structural changes in inflamed liver tissue, which could potentially be used in the diagnostic workup of inflammation in the liver. The presented methods could be implemented within a wider scope computer-aided diagnostics system in a very straightforward manner.",
publisher = "Elsevier Ltd",
journal = "Biomedical Signal Processing & Control",
title = "Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils",
volume = "61",
doi = "10.1016/j.bspc.2020.101959"
}
Oprić, D., Stankovich, A.D., Nenadović, A., Kovačević, S., Obradović, D.D., de Luka, S. R., Nešović-Ostojić, J., Milašin, J., Ilić, A.Ž.,& Trbovich, A. M.. (2020). Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils. in Biomedical Signal Processing & Control
Elsevier Ltd., 61.
https://doi.org/10.1016/j.bspc.2020.101959
Oprić D, Stankovich A, Nenadović A, Kovačević S, Obradović D, de Luka SR, Nešović-Ostojić J, Milašin J, Ilić A, Trbovich AM. Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils. in Biomedical Signal Processing & Control. 2020;61.
doi:10.1016/j.bspc.2020.101959 .
Oprić, D., Stankovich, A.D., Nenadović, A., Kovačević, S., Obradović, D.D., de Luka, Silvio R., Nešović-Ostojić, J., Milašin, Jelena, Ilić, A.Ž., Trbovich, Alexander M., "Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils" in Biomedical Signal Processing & Control, 61 (2020),
https://doi.org/10.1016/j.bspc.2020.101959 . .
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Voronoi Decomposition of Cardiovascular Dependency Structures in Different Ambient Conditions: An Entropy Study

Bajić, Dragana; Skorić, Tamara; Milutinović-Smiljanić, Sanja; Japundžić-Žigon, Nina

(Mdpi, Basel, 2019)

TY  - JOUR
AU  - Bajić, Dragana
AU  - Skorić, Tamara
AU  - Milutinović-Smiljanić, Sanja
AU  - Japundžić-Žigon, Nina
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2418
AB  - This paper proposes a method that maps the coupling strength of an arbitrary number of signals D, D >= 2, into a single time series. It is motivated by the inability of multiscale entropy to jointly analyze more than two signals. The coupling strength is determined using the copula density defined over a [0 1](D) copula domain. The copula domain is decomposed into the Voronoi regions, with volumes inversely proportional to the dependency level (coupling strength) of the observed joint signals. A stream of dependency levels, ordered in time, creates a new time series that shows the fluctuation of the signals' coupling strength along the time axis. The composite multiscale entropy (CMSE) is then applied to three signals, systolic blood pressure (SBP), pulse interval (PI), and body temperature (t(B)), simultaneously recorded from rats exposed to different ambient temperatures (t(A)). The obtained results are consistent with the results from the classical studies, and the method itself offers more levels of freedom than the classical analysis.
PB  - Mdpi, Basel
T2  - Entropy
T1  - Voronoi Decomposition of Cardiovascular Dependency Structures in Different Ambient Conditions: An Entropy Study
VL  - 21
IS  - 11
DO  - 10.3390/e21111103
ER  - 
@article{
author = "Bajić, Dragana and Skorić, Tamara and Milutinović-Smiljanić, Sanja and Japundžić-Žigon, Nina",
year = "2019",
abstract = "This paper proposes a method that maps the coupling strength of an arbitrary number of signals D, D >= 2, into a single time series. It is motivated by the inability of multiscale entropy to jointly analyze more than two signals. The coupling strength is determined using the copula density defined over a [0 1](D) copula domain. The copula domain is decomposed into the Voronoi regions, with volumes inversely proportional to the dependency level (coupling strength) of the observed joint signals. A stream of dependency levels, ordered in time, creates a new time series that shows the fluctuation of the signals' coupling strength along the time axis. The composite multiscale entropy (CMSE) is then applied to three signals, systolic blood pressure (SBP), pulse interval (PI), and body temperature (t(B)), simultaneously recorded from rats exposed to different ambient temperatures (t(A)). The obtained results are consistent with the results from the classical studies, and the method itself offers more levels of freedom than the classical analysis.",
publisher = "Mdpi, Basel",
journal = "Entropy",
title = "Voronoi Decomposition of Cardiovascular Dependency Structures in Different Ambient Conditions: An Entropy Study",
volume = "21",
number = "11",
doi = "10.3390/e21111103"
}
Bajić, D., Skorić, T., Milutinović-Smiljanić, S.,& Japundžić-Žigon, N.. (2019). Voronoi Decomposition of Cardiovascular Dependency Structures in Different Ambient Conditions: An Entropy Study. in Entropy
Mdpi, Basel., 21(11).
https://doi.org/10.3390/e21111103
Bajić D, Skorić T, Milutinović-Smiljanić S, Japundžić-Žigon N. Voronoi Decomposition of Cardiovascular Dependency Structures in Different Ambient Conditions: An Entropy Study. in Entropy. 2019;21(11).
doi:10.3390/e21111103 .
Bajić, Dragana, Skorić, Tamara, Milutinović-Smiljanić, Sanja, Japundžić-Žigon, Nina, "Voronoi Decomposition of Cardiovascular Dependency Structures in Different Ambient Conditions: An Entropy Study" in Entropy, 21, no. 11 (2019),
https://doi.org/10.3390/e21111103 . .
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Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy

Vasić, Marko; Loncar-Turukalo, Tatjana; Tasić, Tatjana; Matić, Marija; Glumac, Sofija; Bajić, Dragana; Popović, Branka; Japundžić-Žigon, Nina

(Academic Press Inc Elsevier Science, San Diego, 2019)

TY  - JOUR
AU  - Vasić, Marko
AU  - Loncar-Turukalo, Tatjana
AU  - Tasić, Tatjana
AU  - Matić, Marija
AU  - Glumac, Sofija
AU  - Bajić, Dragana
AU  - Popović, Branka
AU  - Japundžić-Žigon, Nina
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2469
AB  - Using comprehensive analysis of heart rate (HRV) and blood pressure (BPV) short-term variability we estimated the time course of changes of autonomic nervous system remodeling in two stages of doxorubicin-induced cardiomyopathy (DCM). We also investigated the level of gene expression of cardiac beta-1 (beta-1AR) and beta-2 (beta-2AR) adrenoceptors. Experiments were performed in adult male Wistar rats equipped with indwelling catheters for BP recording and blood withdrawal. A 15 mg/kg total cumulative dose of doxorubicin was injected i.p. to rats to induce DCM or saline for control (n = 18). Rats were assessed for general toxicity, cardiovascular hemodynamic and echocardiography before treatment (n = 6), 35 days (DOX35; n = 6) and 70 days (DOX70; n = 6) post-treatment. HRV was evaluated by spectral analysis, Poincare plots, sample and approximate entropy. Expression of beta-1AR and beta-2AR mRNA was evaluated by RT-qPCR. Doxorubicin-treated rats exhibited poor general condition and lower survival than saline-treated rats. In DOX35 rats, there were no echocardiography signs of decompensation, no increase in serum cardiac troponins, but there was an increase of HRV and decrease of HR complexity. In these rats typical microscopic signs of cardiotoxicity were seen along with over-expression of beta-1AR mRNA. 70 days post-treatment echocardiography revealed signs of decompensation and serum cardiac troponin T was increased. At this stage BPV decreased. In conclusion, HRV increase matches transient over expression of cardiac beta-1AR mRNA in compensate stage of DCM while decompensate stage of DCM is characterized by a decrease of BPV and no changes in beta-1AR and beta-2AR gene expression.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Toxicology & Applied Pharmacology
T1  - Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy
VL  - 362
SP  - 43
EP  - 51
DO  - 10.1016/j.taap.2018.10.015
ER  - 
@article{
author = "Vasić, Marko and Loncar-Turukalo, Tatjana and Tasić, Tatjana and Matić, Marija and Glumac, Sofija and Bajić, Dragana and Popović, Branka and Japundžić-Žigon, Nina",
year = "2019",
abstract = "Using comprehensive analysis of heart rate (HRV) and blood pressure (BPV) short-term variability we estimated the time course of changes of autonomic nervous system remodeling in two stages of doxorubicin-induced cardiomyopathy (DCM). We also investigated the level of gene expression of cardiac beta-1 (beta-1AR) and beta-2 (beta-2AR) adrenoceptors. Experiments were performed in adult male Wistar rats equipped with indwelling catheters for BP recording and blood withdrawal. A 15 mg/kg total cumulative dose of doxorubicin was injected i.p. to rats to induce DCM or saline for control (n = 18). Rats were assessed for general toxicity, cardiovascular hemodynamic and echocardiography before treatment (n = 6), 35 days (DOX35; n = 6) and 70 days (DOX70; n = 6) post-treatment. HRV was evaluated by spectral analysis, Poincare plots, sample and approximate entropy. Expression of beta-1AR and beta-2AR mRNA was evaluated by RT-qPCR. Doxorubicin-treated rats exhibited poor general condition and lower survival than saline-treated rats. In DOX35 rats, there were no echocardiography signs of decompensation, no increase in serum cardiac troponins, but there was an increase of HRV and decrease of HR complexity. In these rats typical microscopic signs of cardiotoxicity were seen along with over-expression of beta-1AR mRNA. 70 days post-treatment echocardiography revealed signs of decompensation and serum cardiac troponin T was increased. At this stage BPV decreased. In conclusion, HRV increase matches transient over expression of cardiac beta-1AR mRNA in compensate stage of DCM while decompensate stage of DCM is characterized by a decrease of BPV and no changes in beta-1AR and beta-2AR gene expression.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Toxicology & Applied Pharmacology",
title = "Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy",
volume = "362",
pages = "43-51",
doi = "10.1016/j.taap.2018.10.015"
}
Vasić, M., Loncar-Turukalo, T., Tasić, T., Matić, M., Glumac, S., Bajić, D., Popović, B.,& Japundžić-Žigon, N.. (2019). Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy. in Toxicology & Applied Pharmacology
Academic Press Inc Elsevier Science, San Diego., 362, 43-51.
https://doi.org/10.1016/j.taap.2018.10.015
Vasić M, Loncar-Turukalo T, Tasić T, Matić M, Glumac S, Bajić D, Popović B, Japundžić-Žigon N. Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy. in Toxicology & Applied Pharmacology. 2019;362:43-51.
doi:10.1016/j.taap.2018.10.015 .
Vasić, Marko, Loncar-Turukalo, Tatjana, Tasić, Tatjana, Matić, Marija, Glumac, Sofija, Bajić, Dragana, Popović, Branka, Japundžić-Žigon, Nina, "Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy" in Toxicology & Applied Pharmacology, 362 (2019):43-51,
https://doi.org/10.1016/j.taap.2018.10.015 . .
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Copula as a dynamic measure of cardiovascular signal interactions

Jovanović, Slađana; Skorić, Tamara; Sarenac, Olivera; Milutinović-Smiljanić, Sanja; Japundžić-Žigon, Nina; Bajić, Dragana

(Elsevier Sci Ltd, Oxford, 2018)

TY  - JOUR
AU  - Jovanović, Slađana
AU  - Skorić, Tamara
AU  - Sarenac, Olivera
AU  - Milutinović-Smiljanić, Sanja
AU  - Japundžić-Žigon, Nina
AU  - Bajić, Dragana
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2291
AB  - Objectives: Copula is a tool for measuring linear and non-linear interactions between two or more time series. The aim of this paper is to prove that a copula approach can accurately capture and visualize the spatial and temporal fluctuations in dependency structures of cardiovascular signals, and to outline the application possibilities. Methods: The method for measuring the level of interaction between systolic blood pressure and the corresponding pulse interval is validated statistically and pharmacologically. The time series are recorded from the freely moving male Wistar rats equipped with radio-telemetry device for blood pressure recording, before and after administration of autonomic blockers scopolamine, atenolol, prazosin and hexamethonium. Implicit (Gaussian and t) and explicit (Clayton, Frank and Gumbel) copulas were calculated and compared to the conventional bivariate methods (Kendal, Pearson, Spearman and classical correlation). Further statistical validation was done using artificially generated surrogate data. A window sliding procedure for dynamic monitoring the signals' coupling strength is implemented. Results: Under the baseline physiological conditions, SBP-PI dependency is significant for time lags 0 s-4 s. Hexamethonium completely abolished the dependency, scopolamine abolished it for time lags 0 s-2 s, atenolol first slightly increased, than for lags greater than 2 s decreased the dependency and prazosin had no effect. Isospectral and isodistributional surrogate data tests confirm that copulas successfully notify the absence of dependency as well. Conclusion: Copula approach accurately captures the temporal fluctuations in dependency structures of SBP and PI, simultaneously enabling a visualization of dependency levels within the particular signal zones. An analysis showed that copulas are more sensitive than the conventional statistical measures, with Frank copula exhibiting the best characterization of SBP and PI dependency.
PB  - Elsevier Sci Ltd, Oxford
T2  - Biomedical Signal Processing & Control
T1  - Copula as a dynamic measure of cardiovascular signal interactions
VL  - 43
SP  - 250
EP  - 264
DO  - 10.1016/j.bspc.2018.03.007
ER  - 
@article{
author = "Jovanović, Slađana and Skorić, Tamara and Sarenac, Olivera and Milutinović-Smiljanić, Sanja and Japundžić-Žigon, Nina and Bajić, Dragana",
year = "2018",
abstract = "Objectives: Copula is a tool for measuring linear and non-linear interactions between two or more time series. The aim of this paper is to prove that a copula approach can accurately capture and visualize the spatial and temporal fluctuations in dependency structures of cardiovascular signals, and to outline the application possibilities. Methods: The method for measuring the level of interaction between systolic blood pressure and the corresponding pulse interval is validated statistically and pharmacologically. The time series are recorded from the freely moving male Wistar rats equipped with radio-telemetry device for blood pressure recording, before and after administration of autonomic blockers scopolamine, atenolol, prazosin and hexamethonium. Implicit (Gaussian and t) and explicit (Clayton, Frank and Gumbel) copulas were calculated and compared to the conventional bivariate methods (Kendal, Pearson, Spearman and classical correlation). Further statistical validation was done using artificially generated surrogate data. A window sliding procedure for dynamic monitoring the signals' coupling strength is implemented. Results: Under the baseline physiological conditions, SBP-PI dependency is significant for time lags 0 s-4 s. Hexamethonium completely abolished the dependency, scopolamine abolished it for time lags 0 s-2 s, atenolol first slightly increased, than for lags greater than 2 s decreased the dependency and prazosin had no effect. Isospectral and isodistributional surrogate data tests confirm that copulas successfully notify the absence of dependency as well. Conclusion: Copula approach accurately captures the temporal fluctuations in dependency structures of SBP and PI, simultaneously enabling a visualization of dependency levels within the particular signal zones. An analysis showed that copulas are more sensitive than the conventional statistical measures, with Frank copula exhibiting the best characterization of SBP and PI dependency.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Biomedical Signal Processing & Control",
title = "Copula as a dynamic measure of cardiovascular signal interactions",
volume = "43",
pages = "250-264",
doi = "10.1016/j.bspc.2018.03.007"
}
Jovanović, S., Skorić, T., Sarenac, O., Milutinović-Smiljanić, S., Japundžić-Žigon, N.,& Bajić, D.. (2018). Copula as a dynamic measure of cardiovascular signal interactions. in Biomedical Signal Processing & Control
Elsevier Sci Ltd, Oxford., 43, 250-264.
https://doi.org/10.1016/j.bspc.2018.03.007
Jovanović S, Skorić T, Sarenac O, Milutinović-Smiljanić S, Japundžić-Žigon N, Bajić D. Copula as a dynamic measure of cardiovascular signal interactions. in Biomedical Signal Processing & Control. 2018;43:250-264.
doi:10.1016/j.bspc.2018.03.007 .
Jovanović, Slađana, Skorić, Tamara, Sarenac, Olivera, Milutinović-Smiljanić, Sanja, Japundžić-Žigon, Nina, Bajić, Dragana, "Copula as a dynamic measure of cardiovascular signal interactions" in Biomedical Signal Processing & Control, 43 (2018):250-264,
https://doi.org/10.1016/j.bspc.2018.03.007 . .
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Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation

Stanković, Marija; Pantić, Igor; de Luka, Silvio R.; Puškaš, Nela; Zaletel, Ivan; Milutinović-Smiljanić, Sanja; Pantić, Senka; Trbovich, Alexander M.

(Wiley, Hoboken, 2016)

TY  - JOUR
AU  - Stanković, Marija
AU  - Pantić, Igor
AU  - de Luka, Silvio R.
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - Milutinović-Smiljanić, Sanja
AU  - Pantić, Senka
AU  - Trbovich, Alexander M.
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2129
AB  - The aim of the study was to examine alteration and possible application of fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely inflamed tissue. Acute inflammation was induced by injection of turpentine oil into the right and left hind limb muscles of mice, whereas control animals received intramuscular saline injection. After 12h, animals were anesthetised and treated muscles collected. The tissue was stained by hematoxylin and eosin, digital micrographs produced, enabling determination of fractal dimension of the cells, angular second moment and correlation of studied tissue. Histopathological analysis showed presence of inflammatory infiltrate and tissue damage in inflammatory group, whereas tissue structure in control group was preserved, devoid of inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of treated tissue in inflammatory group decreased in comparison to the control group. In this study, we were first to observe and report that fractal dimension of the cells, angular second moment, and correlation were reduced in acutely inflamed tissue, indicating loss of overall complexity of the cells in the tissue, the tissue uniformity and structure regularity. Fractal dimension, angular second moment and correlation could be useful methods for quantification of structural changes in acute inflammation. Lay Description The aim of this study was to examine alteration, and possible application of mathematical parameters fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely-inflamed tissue. An acute inflammation was induced by injection of turpentine oil into mice muscles, whereas control group received intramuscular injection of saline. After 12 h animals were anesthetized, and treated muscles were collected. The tissue was stained, and photos of the tissue were made. Mathematical parameters, namely fractal dimension, angular second moment, and correlation of the tissue photo, were determined by computer program. Standard histopathological analysis showed that inflammatory infiltrate and tissue damage were present in inflammatory group, whereas tissue structure in control group was preserved, and without inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of the treated tissue in inflammatory group decreased, when compared to control group. In this study we reported, for the first time, that fractal dimension of the cells, angular second moment, and correlation had decreased in acutely-inflamed tissue, indicating loss of overall complexity of cells in tissue, tissue uniformity, and structure regularity. Fractal dimension, angular second moment, and correlation could be useful methods for quantification of structural changes in acute inflammation.
PB  - Wiley, Hoboken
T2  - Journal of Microscopy
T1  - Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation
VL  - 261
IS  - 3
SP  - 277
EP  - 284
DO  - 10.1111/jmi.12330
ER  - 
@article{
author = "Stanković, Marija and Pantić, Igor and de Luka, Silvio R. and Puškaš, Nela and Zaletel, Ivan and Milutinović-Smiljanić, Sanja and Pantić, Senka and Trbovich, Alexander M.",
year = "2016",
abstract = "The aim of the study was to examine alteration and possible application of fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely inflamed tissue. Acute inflammation was induced by injection of turpentine oil into the right and left hind limb muscles of mice, whereas control animals received intramuscular saline injection. After 12h, animals were anesthetised and treated muscles collected. The tissue was stained by hematoxylin and eosin, digital micrographs produced, enabling determination of fractal dimension of the cells, angular second moment and correlation of studied tissue. Histopathological analysis showed presence of inflammatory infiltrate and tissue damage in inflammatory group, whereas tissue structure in control group was preserved, devoid of inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of treated tissue in inflammatory group decreased in comparison to the control group. In this study, we were first to observe and report that fractal dimension of the cells, angular second moment, and correlation were reduced in acutely inflamed tissue, indicating loss of overall complexity of the cells in the tissue, the tissue uniformity and structure regularity. Fractal dimension, angular second moment and correlation could be useful methods for quantification of structural changes in acute inflammation. Lay Description The aim of this study was to examine alteration, and possible application of mathematical parameters fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely-inflamed tissue. An acute inflammation was induced by injection of turpentine oil into mice muscles, whereas control group received intramuscular injection of saline. After 12 h animals were anesthetized, and treated muscles were collected. The tissue was stained, and photos of the tissue were made. Mathematical parameters, namely fractal dimension, angular second moment, and correlation of the tissue photo, were determined by computer program. Standard histopathological analysis showed that inflammatory infiltrate and tissue damage were present in inflammatory group, whereas tissue structure in control group was preserved, and without inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of the treated tissue in inflammatory group decreased, when compared to control group. In this study we reported, for the first time, that fractal dimension of the cells, angular second moment, and correlation had decreased in acutely-inflamed tissue, indicating loss of overall complexity of cells in tissue, tissue uniformity, and structure regularity. Fractal dimension, angular second moment, and correlation could be useful methods for quantification of structural changes in acute inflammation.",
publisher = "Wiley, Hoboken",
journal = "Journal of Microscopy",
title = "Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation",
volume = "261",
number = "3",
pages = "277-284",
doi = "10.1111/jmi.12330"
}
Stanković, M., Pantić, I., de Luka, S. R., Puškaš, N., Zaletel, I., Milutinović-Smiljanić, S., Pantić, S.,& Trbovich, A. M.. (2016). Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation. in Journal of Microscopy
Wiley, Hoboken., 261(3), 277-284.
https://doi.org/10.1111/jmi.12330
Stanković M, Pantić I, de Luka SR, Puškaš N, Zaletel I, Milutinović-Smiljanić S, Pantić S, Trbovich AM. Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation. in Journal of Microscopy. 2016;261(3):277-284.
doi:10.1111/jmi.12330 .
Stanković, Marija, Pantić, Igor, de Luka, Silvio R., Puškaš, Nela, Zaletel, Ivan, Milutinović-Smiljanić, Sanja, Pantić, Senka, Trbovich, Alexander M., "Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation" in Journal of Microscopy, 261, no. 3 (2016):277-284,
https://doi.org/10.1111/jmi.12330 . .
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Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation

Stanković, Marija S.; Turuntas, Vladimir; de Luka, Silvio R.; Janković, Saša; Stefanović, Srđan; Puškaš, Nela; Zaletel, Ivan; Milutinović-Smiljanić, Sanja; Trbovich, Alexander M.

(Academic Press Inc Elsevier Science, San Diego, 2015)

TY  - JOUR
AU  - Stanković, Marija S.
AU  - Turuntas, Vladimir
AU  - de Luka, Silvio R.
AU  - Janković, Saša
AU  - Stefanović, Srđan
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - Milutinović-Smiljanić, Sanja
AU  - Trbovich, Alexander M.
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2003
AB  - Aim: The aim of this study was to examine the role of the IL-33/ST2 pathway in pathogenesis of acute inflammation by investigating its possible role in alteration of iron and hematological parameters in experimental model of acute inflammation. Material and methods: Wild-type and ST2 knockout BALB/c mice were divided into four groups: wild-type control group, ST2-/- control group, wild-type inflammatory group, and ST2-/- inflammatory group. Acute inflammation was induced by intramuscular injection of turpentine oil, while control groups were injected with saline. After 12 h animals were anesthetized, and the treated tissue, blood and spleen were collected. Iron concentration in the treated tissue, hemoglobin blood concentration, mean corpuscular hemoglobin (MCH), hematocrit, erythrocyte, neutrophil and lymphocyte blood count, and erythrocytes percentage in spleen were determined. Results: Iron concentration in the treated tissue was significantly higher in wild-type inflammatory group (WT-I) when compared to both, the wild-type control group (WT-C) and ST2-/- inflammatory group (KO-I). There was no significant difference in iron concentration between ST2-/- control group (KO-C) and the KO-I. MCH had significantly decreased in WT-I when compared to WT-C, while there was no significant difference between KO-C and KO-I. Hemoglobin blood concentration significantly increased in KO-I in comparison to KO-C, while it did not significantly differ between WT-I and KO-I. Erythrocyte count and hematocrit had significantly increased, while the percentage of erythrocytes in spleen decreased in both inflammatory groups when compared to their controls. Neutrophil count significantly decreased in WT-I, when compared to WT-C. Lymphocyte count decreased in both inflammatory groups when compared to their controls. Conclusion: Results of this study indicate that the IL-33/ST2 axis could have a role in the alteration of iron in acute inflammation, namely in an increase of iron concentration at the site of acute inflammation and a decrease of blood mean corpuscular hemoglobin.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Experimental & Molecular Pathology
T1  - Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation
VL  - 99
IS  - 3
SP  - 687
EP  - 692
DO  - 10.1016/j.yexmp.2015.11.016
ER  - 
@article{
author = "Stanković, Marija S. and Turuntas, Vladimir and de Luka, Silvio R. and Janković, Saša and Stefanović, Srđan and Puškaš, Nela and Zaletel, Ivan and Milutinović-Smiljanić, Sanja and Trbovich, Alexander M.",
year = "2015",
abstract = "Aim: The aim of this study was to examine the role of the IL-33/ST2 pathway in pathogenesis of acute inflammation by investigating its possible role in alteration of iron and hematological parameters in experimental model of acute inflammation. Material and methods: Wild-type and ST2 knockout BALB/c mice were divided into four groups: wild-type control group, ST2-/- control group, wild-type inflammatory group, and ST2-/- inflammatory group. Acute inflammation was induced by intramuscular injection of turpentine oil, while control groups were injected with saline. After 12 h animals were anesthetized, and the treated tissue, blood and spleen were collected. Iron concentration in the treated tissue, hemoglobin blood concentration, mean corpuscular hemoglobin (MCH), hematocrit, erythrocyte, neutrophil and lymphocyte blood count, and erythrocytes percentage in spleen were determined. Results: Iron concentration in the treated tissue was significantly higher in wild-type inflammatory group (WT-I) when compared to both, the wild-type control group (WT-C) and ST2-/- inflammatory group (KO-I). There was no significant difference in iron concentration between ST2-/- control group (KO-C) and the KO-I. MCH had significantly decreased in WT-I when compared to WT-C, while there was no significant difference between KO-C and KO-I. Hemoglobin blood concentration significantly increased in KO-I in comparison to KO-C, while it did not significantly differ between WT-I and KO-I. Erythrocyte count and hematocrit had significantly increased, while the percentage of erythrocytes in spleen decreased in both inflammatory groups when compared to their controls. Neutrophil count significantly decreased in WT-I, when compared to WT-C. Lymphocyte count decreased in both inflammatory groups when compared to their controls. Conclusion: Results of this study indicate that the IL-33/ST2 axis could have a role in the alteration of iron in acute inflammation, namely in an increase of iron concentration at the site of acute inflammation and a decrease of blood mean corpuscular hemoglobin.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Experimental & Molecular Pathology",
title = "Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation",
volume = "99",
number = "3",
pages = "687-692",
doi = "10.1016/j.yexmp.2015.11.016"
}
Stanković, M. S., Turuntas, V., de Luka, S. R., Janković, S., Stefanović, S., Puškaš, N., Zaletel, I., Milutinović-Smiljanić, S.,& Trbovich, A. M.. (2015). Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation. in Experimental & Molecular Pathology
Academic Press Inc Elsevier Science, San Diego., 99(3), 687-692.
https://doi.org/10.1016/j.yexmp.2015.11.016
Stanković MS, Turuntas V, de Luka SR, Janković S, Stefanović S, Puškaš N, Zaletel I, Milutinović-Smiljanić S, Trbovich AM. Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation. in Experimental & Molecular Pathology. 2015;99(3):687-692.
doi:10.1016/j.yexmp.2015.11.016 .
Stanković, Marija S., Turuntas, Vladimir, de Luka, Silvio R., Janković, Saša, Stefanović, Srđan, Puškaš, Nela, Zaletel, Ivan, Milutinović-Smiljanić, Sanja, Trbovich, Alexander M., "Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation" in Experimental & Molecular Pathology, 99, no. 3 (2015):687-692,
https://doi.org/10.1016/j.yexmp.2015.11.016 . .
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Evidence for involvement of central vasopressin V1b and V2 receptors in stress-induced baroreflex desensitization

Milutinović-Smiljanić, Sanja; Sarenac, Olivera; Lozic-Đurić, Maja; Murphy, David; Japundžić-Žigon, Nina

(Wiley-Blackwell, Hoboken, 2013)

TY  - JOUR
AU  - Milutinović-Smiljanić, Sanja
AU  - Sarenac, Olivera
AU  - Lozic-Đurić, Maja
AU  - Murphy, David
AU  - Japundžić-Žigon, Nina
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1834
AB  - Background and Purpose It is well recognized that vasopressin modulates the neurogenic control of the circulation. Here, we report the central mechanisms by which vasopressin modulates cardiovascular response to stress induced by immobilization. Experimental Approach Experiments were performed in conscious male Wistar rats equipped with radiotelemetric device for continuous measurement of haemodynamic parameters: systolic and diastolic BP and heart rate (HR). The functioning of the spontaneous baro-receptor reflex (BRR) was evaluated using the sequence method and the following parameters were evaluated: BRR sensitivity (BRS) and BRR effectiveness index (BEI). Key Results Under baseline physiological conditions intracerebroventricular injection of 100 and 500ng of selective non-peptide V1a or V1b or V2 receptor antagonist did not modify BP, HR and BRR. Rats exposed to 15min long stress by immobilization exhibited increase of BP, HR, reduction of BRS and no change in BEI. Pretreatment of rats with V1a receptor antagonist did not modulate the BP, HR, BRS and BEI response to stress. Pretreatment of rats with V1b receptor and V2 receptor antagonist, at both doses, prevented BRR desensitization and tachycardia, but failed to modulate stress-induced hypertension. Conclusions and Implications Vasopressin by the stimulation of central V1b- and V2-like receptors mediates stress-induced tachycardia and BRR desensitization. If these mechanisms are involved, BRR desensitization in heart failure and hypertension associated with poor outcome, they could be considered as novel targets for cardiovascular drug development.
PB  - Wiley-Blackwell, Hoboken
T2  - British Journal of Pharmacology
T1  - Evidence for involvement of central vasopressin V1b and V2 receptors in stress-induced baroreflex desensitization
VL  - 169
IS  - 4
SP  - 900
EP  - 908
DO  - 10.1111/bph.12161
ER  - 
@article{
author = "Milutinović-Smiljanić, Sanja and Sarenac, Olivera and Lozic-Đurić, Maja and Murphy, David and Japundžić-Žigon, Nina",
year = "2013",
abstract = "Background and Purpose It is well recognized that vasopressin modulates the neurogenic control of the circulation. Here, we report the central mechanisms by which vasopressin modulates cardiovascular response to stress induced by immobilization. Experimental Approach Experiments were performed in conscious male Wistar rats equipped with radiotelemetric device for continuous measurement of haemodynamic parameters: systolic and diastolic BP and heart rate (HR). The functioning of the spontaneous baro-receptor reflex (BRR) was evaluated using the sequence method and the following parameters were evaluated: BRR sensitivity (BRS) and BRR effectiveness index (BEI). Key Results Under baseline physiological conditions intracerebroventricular injection of 100 and 500ng of selective non-peptide V1a or V1b or V2 receptor antagonist did not modify BP, HR and BRR. Rats exposed to 15min long stress by immobilization exhibited increase of BP, HR, reduction of BRS and no change in BEI. Pretreatment of rats with V1a receptor antagonist did not modulate the BP, HR, BRS and BEI response to stress. Pretreatment of rats with V1b receptor and V2 receptor antagonist, at both doses, prevented BRR desensitization and tachycardia, but failed to modulate stress-induced hypertension. Conclusions and Implications Vasopressin by the stimulation of central V1b- and V2-like receptors mediates stress-induced tachycardia and BRR desensitization. If these mechanisms are involved, BRR desensitization in heart failure and hypertension associated with poor outcome, they could be considered as novel targets for cardiovascular drug development.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "British Journal of Pharmacology",
title = "Evidence for involvement of central vasopressin V1b and V2 receptors in stress-induced baroreflex desensitization",
volume = "169",
number = "4",
pages = "900-908",
doi = "10.1111/bph.12161"
}
Milutinović-Smiljanić, S., Sarenac, O., Lozic-Đurić, M., Murphy, D.,& Japundžić-Žigon, N.. (2013). Evidence for involvement of central vasopressin V1b and V2 receptors in stress-induced baroreflex desensitization. in British Journal of Pharmacology
Wiley-Blackwell, Hoboken., 169(4), 900-908.
https://doi.org/10.1111/bph.12161
Milutinović-Smiljanić S, Sarenac O, Lozic-Đurić M, Murphy D, Japundžić-Žigon N. Evidence for involvement of central vasopressin V1b and V2 receptors in stress-induced baroreflex desensitization. in British Journal of Pharmacology. 2013;169(4):900-908.
doi:10.1111/bph.12161 .
Milutinović-Smiljanić, Sanja, Sarenac, Olivera, Lozic-Đurić, Maja, Murphy, David, Japundžić-Žigon, Nina, "Evidence for involvement of central vasopressin V1b and V2 receptors in stress-induced baroreflex desensitization" in British Journal of Pharmacology, 169, no. 4 (2013):900-908,
https://doi.org/10.1111/bph.12161 . .
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