TY  - JOUR
AU  - Čairović, Aleksandra
AU  - Stanimirović, Dragan
AU  - Krajnović, Tamara T.
AU  - Dojčinović, Biljana
AU  - Maksimović, Vesna
AU  - Cvijović-Alagić, Ivana
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2386
AB  - The biological quality and chemical composition of alloys used in dental practice change during heat treatment. Often the residues of the previous cast are not disposed of but are reused and recycled until consumed. Thus, manufactured dental restorations have modified biological quality and chemical composition, and compromised biocompatibility. The aim of this study was to investigate the influence of repeated casting on the cytotoxicity of the silver-palladium (Ag-Pd) alloy. Our results showed that repeated casting of the Ag-Pd dental alloy affected its biocompatibility by promoting toxicity against transformed fibroblasts in a contact-independent manner. A strong decrease in cell proliferation, induction of senescence and massive cell death were observed in cultures exposed only to a medium previously incubated with dental alloy samples. The obtained data indicated that toxicity mediated by the accumulation of the Ag, Pd, Cu and Zn cations released from the Ag-Pd material was enhanced by recasting. The induction of cell senescence and subsequent apoptotic and necrotic death were accompanied by amplified intracellular production of reactive oxygen and nitrogen species, suggesting their involvement in the cell destruction process. Therefore, compromised biocompatibility after recasting with the Ag-Pd alloy can be the cause of serious local cell destruction, as observed in clinical practice.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Recasting as a booster of Ag-Pd alloy cytotoxicity: induction of cell senescence prior to mass cell death
VL  - 71
IS  - 2
SP  - 347
EP  - 356
DO  - 10.2298/ABS190305017C
ER  - 

@article{
author = "Čairović, Aleksandra and Stanimirović, Dragan and Krajnović, Tamara T. and Dojčinović, Biljana and Maksimović, Vesna and Cvijović-Alagić, Ivana",
year = "2019",
abstract = "The biological quality and chemical composition of alloys used in dental practice change during heat treatment. Often the residues of the previous cast are not disposed of but are reused and recycled until consumed. Thus, manufactured dental restorations have modified biological quality and chemical composition, and compromised biocompatibility. The aim of this study was to investigate the influence of repeated casting on the cytotoxicity of the silver-palladium (Ag-Pd) alloy. Our results showed that repeated casting of the Ag-Pd dental alloy affected its biocompatibility by promoting toxicity against transformed fibroblasts in a contact-independent manner. A strong decrease in cell proliferation, induction of senescence and massive cell death were observed in cultures exposed only to a medium previously incubated with dental alloy samples. The obtained data indicated that toxicity mediated by the accumulation of the Ag, Pd, Cu and Zn cations released from the Ag-Pd material was enhanced by recasting. The induction of cell senescence and subsequent apoptotic and necrotic death were accompanied by amplified intracellular production of reactive oxygen and nitrogen species, suggesting their involvement in the cell destruction process. Therefore, compromised biocompatibility after recasting with the Ag-Pd alloy can be the cause of serious local cell destruction, as observed in clinical practice.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Recasting as a booster of Ag-Pd alloy cytotoxicity: induction of cell senescence prior to mass cell death",
volume = "71",
number = "2",
pages = "347-356",
doi = "10.2298/ABS190305017C"
}

Čairović, A., Stanimirović, D., Krajnović, T. T., Dojčinović, B., Maksimović, V.,& Cvijović-Alagić, I.. (2019). Recasting as a booster of Ag-Pd alloy cytotoxicity: induction of cell senescence prior to mass cell death. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 71(2), 347-356.
https://doi.org/10.2298/ABS190305017C

Čairović A, Stanimirović D, Krajnović TT, Dojčinović B, Maksimović V, Cvijović-Alagić I. Recasting as a booster of Ag-Pd alloy cytotoxicity: induction of cell senescence prior to mass cell death. in Archives of Biological Sciences. 2019;71(2):347-356.
doi:10.2298/ABS190305017C .

Čairović, Aleksandra, Stanimirović, Dragan, Krajnović, Tamara T., Dojčinović, Biljana, Maksimović, Vesna, Cvijović-Alagić, Ivana, "Recasting as a booster of Ag-Pd alloy cytotoxicity: induction of cell senescence prior to mass cell death" in Archives of Biological Sciences, 71, no. 2 (2019):347-356,
https://doi.org/10.2298/ABS190305017C . .

TY  - JOUR
AU  - Čairović, Aleksandra
AU  - Đorđević, Ivan
AU  - Bulatović, Mirna
AU  - Mojić, Marija
AU  - Momcilović, Miljana
AU  - Stošic-Grujicić, Stanislava
AU  - Maksimović, Vesna
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Stamenković, Dragoslav
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1826
AB  - The aim of this study was to evaluate the effect of recasting of commercially available Ni-Cr (Wiron 99) and Co-Cr (Dentalit C) dental alloys on physiology of microenvironmental cells. The viability of fibrosarcoma (L929) cells, human embrional fibroblasts (MRC-5) and isolated peripheral blood mononuclear cells (PBMC) was measured by MTT and acidic phosphatase tests. Presence of dying cells was estimated by Annexin/PI staining while the production of intracellular nitric oxide (NO), reactive oxygen (ROS) and nitrogen (RNS) species was determined by DAF-FM diacetate and DHR staining. Recasting of Ni-Cr alloy intensified its cytotoxicity manifested through enhanced free radicals production, induction of cell death and permamently diminished cell proliferation. On the other hand, after initial toxic effect cells adapted to the presence of Co-Cr alloys. Independently of recasting, Co-Cr alloys are more compatible with microenvironment then Ni-Cr alloy. Oppositely, recasting of Ni-Cr alloy promoted its toxicity.
T2  - Digest Journal of Nanomaterials & Biostructures
T1  - In vitro assessment of ni-cr and co-cr dental alloys upon recasting: cellular compatibility
VL  - 8
IS  - 2
SP  - 877
EP  - 886
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5620
ER  - 

@article{
author = "Čairović, Aleksandra and Đorđević, Ivan and Bulatović, Mirna and Mojić, Marija and Momcilović, Miljana and Stošic-Grujicić, Stanislava and Maksimović, Vesna and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Stamenković, Dragoslav",
year = "2013",
abstract = "The aim of this study was to evaluate the effect of recasting of commercially available Ni-Cr (Wiron 99) and Co-Cr (Dentalit C) dental alloys on physiology of microenvironmental cells. The viability of fibrosarcoma (L929) cells, human embrional fibroblasts (MRC-5) and isolated peripheral blood mononuclear cells (PBMC) was measured by MTT and acidic phosphatase tests. Presence of dying cells was estimated by Annexin/PI staining while the production of intracellular nitric oxide (NO), reactive oxygen (ROS) and nitrogen (RNS) species was determined by DAF-FM diacetate and DHR staining. Recasting of Ni-Cr alloy intensified its cytotoxicity manifested through enhanced free radicals production, induction of cell death and permamently diminished cell proliferation. On the other hand, after initial toxic effect cells adapted to the presence of Co-Cr alloys. Independently of recasting, Co-Cr alloys are more compatible with microenvironment then Ni-Cr alloy. Oppositely, recasting of Ni-Cr alloy promoted its toxicity.",
journal = "Digest Journal of Nanomaterials & Biostructures",
title = "In vitro assessment of ni-cr and co-cr dental alloys upon recasting: cellular compatibility",
volume = "8",
number = "2",
pages = "877-886",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5620"
}

Čairović, A., Đorđević, I., Bulatović, M., Mojić, M., Momcilović, M., Stošic-Grujicić, S., Maksimović, V., Maksimović-Ivanić, D., Mijatović, S.,& Stamenković, D.. (2013). In vitro assessment of ni-cr and co-cr dental alloys upon recasting: cellular compatibility. in Digest Journal of Nanomaterials & Biostructures, 8(2), 877-886.
https://hdl.handle.net/21.15107/rcub_vinar_5620

Čairović A, Đorđević I, Bulatović M, Mojić M, Momcilović M, Stošic-Grujicić S, Maksimović V, Maksimović-Ivanić D, Mijatović S, Stamenković D. In vitro assessment of ni-cr and co-cr dental alloys upon recasting: cellular compatibility. in Digest Journal of Nanomaterials & Biostructures. 2013;8(2):877-886.
https://hdl.handle.net/21.15107/rcub_vinar_5620 .

Čairović, Aleksandra, Đorđević, Ivan, Bulatović, Mirna, Mojić, Marija, Momcilović, Miljana, Stošic-Grujicić, Stanislava, Maksimović, Vesna, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Stamenković, Dragoslav, "In vitro assessment of ni-cr and co-cr dental alloys upon recasting: cellular compatibility" in Digest Journal of Nanomaterials & Biostructures, 8, no. 2 (2013):877-886,
https://hdl.handle.net/21.15107/rcub_vinar_5620 .