Chemical and structural designing of nanomaterials for application in medicine and tissue engineering

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Chemical and structural designing of nanomaterials for application in medicine and tissue engineering (en)
Хемијско и структурно дизајнирање наноматеријала за примену у медицини и инжењерству ткива (sr)
Hemijsko i strukturno dizajniranje nanomaterijala za primenu u medicini i inženjerstvu tkiva (sr_RS)
Authors

Publications

Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats

Paraš, S.; Trišić, Dijana; Ajtić, O.M.; Prokić, B.; Drobne, D.; Živković, Slavoljub; Jokanović, Vukoman

(Mdpi Ag, 2020)

TY  - JOUR
AU  - Paraš, S.
AU  - Trišić, Dijana
AU  - Ajtić, O.M.
AU  - Prokić, B.
AU  - Drobne, D.
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2478
AB  - Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.
PB  - Mdpi Ag
T2  - Nanomaterials
T1  - Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats
VL  - 10
IS  - 5
DO  - 10.3390/nano10050918
ER  - 
@article{
author = "Paraš, S. and Trišić, Dijana and Ajtić, O.M. and Prokić, B. and Drobne, D. and Živković, Slavoljub and Jokanović, Vukoman",
year = "2020",
abstract = "Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.",
publisher = "Mdpi Ag",
journal = "Nanomaterials",
title = "Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats",
volume = "10",
number = "5",
doi = "10.3390/nano10050918"
}
Paraš, S., Trišić, D., Ajtić, O.M., Prokić, B., Drobne, D., Živković, S.,& Jokanović, V.. (2020). Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats. in Nanomaterials
Mdpi Ag., 10(5).
https://doi.org/10.3390/nano10050918
Paraš S, Trišić D, Ajtić O, Prokić B, Drobne D, Živković S, Jokanović V. Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats. in Nanomaterials. 2020;10(5).
doi:10.3390/nano10050918 .
Paraš, S., Trišić, Dijana, Ajtić, O.M., Prokić, B., Drobne, D., Živković, Slavoljub, Jokanović, Vukoman, "Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats" in Nanomaterials, 10, no. 5 (2020),
https://doi.org/10.3390/nano10050918 . .
3
3
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Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation: A preliminary study

Ćetenović, Bojana; Marković, Dejan; Gatman, Džejms; Perić, Tamara; Jokanović, Vukoman

(Srpsko lekarsko društvo, Beograd, 2019)

TY  - JOUR
AU  - Ćetenović, Bojana
AU  - Marković, Dejan
AU  - Gatman, Džejms
AU  - Perić, Tamara
AU  - Jokanović, Vukoman
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2438
AB  - Introduction/Objective the purpose of this study was to assess effectiveness of endodontic root canal procedures in traumatized permanent teeth with necrotic pulps and chronic periapical lesions after definitive obturation with mineral trioxide aggregate (MTA) products. Adobe Photoshop CS (San Jose, CA, USA) image-analysis software was used for healing assessment. Methods Twenty-seven traumatized single-rooted permanent teeth diagnosed with necrotic pulps and chronic periapical lesions were treated with non-surgical procedure using a tri-antibiotic paste and calcium hydroxide as intra-canal medication. Definitive obturation was performed with ProRoot MTA (Dentsply Tulsa Dental Specialties, Tulsa, OK, USA) or MTA+ Cerkamed (Cerkamed, Stalowa Wola, Poland). Control follow-ups were done three, six, 12, and 24 months following the completion of treatment. Results the positive clinical outcome was recorded in 24 (88.9%) cases, while radiographic success was present in 26 (96.3%) cases. A statistically significant decrease in the sizes of periapical lesions was consistently observed at follow-up periods (p  lt  0.001). There was no statistically significant difference between the two tested MTA materials (p > 0.05). Conclusion the MTA products were effective for the root canal obturation and appeared to contribute to the significant reduction or complete regression of periapical lesions in teeth treated. The presented procedure may be proposed for everyday clinical practice.
AB  - Uvod/Cilj Svrha ove studije bila je procena efikasnosti endodontskog tretmana traumatizovanih stalnih zuba sa nekrotičnom pulpom i hroničnim periapikalnim lezijama posle definitivne opturacije mineralnim trioksidnim agregatom (MTA). Softver za analizu slike Adobe Photoshop CS korišćen je za procenu regeneracije. Metode Dvadeset i sedam traumatizovanih jednokorenih stalnih zuba sa nekrotičnom pulpom i hroničnim periapikalnim lezijama tretirani su nehirurškim procedurama pomoću triantibiotske paste i kalcijum-hidroksida kao intrakanalnog medikamenta. Definitivna opturacija izvedena je pomoću ProRoot MTA ili MTA+ Cerkamed. Kontrolni pregledi obavljeni su tri, šest, 12 i 24 meseca posle završetka lečenja. Rezultati Pozitivan klinički ishod zabeležen je u 24 (88,9%) slučaja, dok je radiografski uspeh bio prisutan u 26 (96,3%) slučaja. Statistički značajno smanjenje veličine periapikalnih lezija primećeno je u svim periodima (p  lt  0,001). Nije bilo statistički značajne razlike između dva testirana MTA materijala (p > 0,05). Zaključak MTA proizvodi su efikasni u opturaciji korenskih kanala i čini se da doprinose značajnom smanjenju ili potpunoj regresiji periapikalnih lezija u tretiranim zubima. Prikazana metodologija može biti predložena za svakodnevnu kliničku praksu.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation: A preliminary study
T1  - Endodontsko lečenje traumatizovanih zuba sa hroničnim periapikalnim lezijama primenom antibiotske paste i opturacije mineralnim trioksidnim agregatom - preliminarna studija
VL  - 147
IS  - 5-6
SP  - 270
EP  - 275
DO  - 10.2298/SARH180301018C
ER  - 
@article{
author = "Ćetenović, Bojana and Marković, Dejan and Gatman, Džejms and Perić, Tamara and Jokanović, Vukoman",
year = "2019",
abstract = "Introduction/Objective the purpose of this study was to assess effectiveness of endodontic root canal procedures in traumatized permanent teeth with necrotic pulps and chronic periapical lesions after definitive obturation with mineral trioxide aggregate (MTA) products. Adobe Photoshop CS (San Jose, CA, USA) image-analysis software was used for healing assessment. Methods Twenty-seven traumatized single-rooted permanent teeth diagnosed with necrotic pulps and chronic periapical lesions were treated with non-surgical procedure using a tri-antibiotic paste and calcium hydroxide as intra-canal medication. Definitive obturation was performed with ProRoot MTA (Dentsply Tulsa Dental Specialties, Tulsa, OK, USA) or MTA+ Cerkamed (Cerkamed, Stalowa Wola, Poland). Control follow-ups were done three, six, 12, and 24 months following the completion of treatment. Results the positive clinical outcome was recorded in 24 (88.9%) cases, while radiographic success was present in 26 (96.3%) cases. A statistically significant decrease in the sizes of periapical lesions was consistently observed at follow-up periods (p  lt  0.001). There was no statistically significant difference between the two tested MTA materials (p > 0.05). Conclusion the MTA products were effective for the root canal obturation and appeared to contribute to the significant reduction or complete regression of periapical lesions in teeth treated. The presented procedure may be proposed for everyday clinical practice., Uvod/Cilj Svrha ove studije bila je procena efikasnosti endodontskog tretmana traumatizovanih stalnih zuba sa nekrotičnom pulpom i hroničnim periapikalnim lezijama posle definitivne opturacije mineralnim trioksidnim agregatom (MTA). Softver za analizu slike Adobe Photoshop CS korišćen je za procenu regeneracije. Metode Dvadeset i sedam traumatizovanih jednokorenih stalnih zuba sa nekrotičnom pulpom i hroničnim periapikalnim lezijama tretirani su nehirurškim procedurama pomoću triantibiotske paste i kalcijum-hidroksida kao intrakanalnog medikamenta. Definitivna opturacija izvedena je pomoću ProRoot MTA ili MTA+ Cerkamed. Kontrolni pregledi obavljeni su tri, šest, 12 i 24 meseca posle završetka lečenja. Rezultati Pozitivan klinički ishod zabeležen je u 24 (88,9%) slučaja, dok je radiografski uspeh bio prisutan u 26 (96,3%) slučaja. Statistički značajno smanjenje veličine periapikalnih lezija primećeno je u svim periodima (p  lt  0,001). Nije bilo statistički značajne razlike između dva testirana MTA materijala (p > 0,05). Zaključak MTA proizvodi su efikasni u opturaciji korenskih kanala i čini se da doprinose značajnom smanjenju ili potpunoj regresiji periapikalnih lezija u tretiranim zubima. Prikazana metodologija može biti predložena za svakodnevnu kliničku praksu.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation: A preliminary study, Endodontsko lečenje traumatizovanih zuba sa hroničnim periapikalnim lezijama primenom antibiotske paste i opturacije mineralnim trioksidnim agregatom - preliminarna studija",
volume = "147",
number = "5-6",
pages = "270-275",
doi = "10.2298/SARH180301018C"
}
Ćetenović, B., Marković, D., Gatman, D., Perić, T.,& Jokanović, V.. (2019). Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation: A preliminary study. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 147(5-6), 270-275.
https://doi.org/10.2298/SARH180301018C
Ćetenović B, Marković D, Gatman D, Perić T, Jokanović V. Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation: A preliminary study. in Srpski arhiv za celokupno lekarstvo. 2019;147(5-6):270-275.
doi:10.2298/SARH180301018C .
Ćetenović, Bojana, Marković, Dejan, Gatman, Džejms, Perić, Tamara, Jokanović, Vukoman, "Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation: A preliminary study" in Srpski arhiv za celokupno lekarstvo, 147, no. 5-6 (2019):270-275,
https://doi.org/10.2298/SARH180301018C . .
4
3
3

Challenges in experimental evaluation of morphological, chemo-mechanical and adhesive properties of glass-ionomer based dental materials

Petrović, Bojan; Marković, Evgenija; Perić, Tamara; Kojić, Sanja

(2019)

TY  - JOUR
AU  - Petrović, Bojan
AU  - Marković, Evgenija
AU  - Perić, Tamara
AU  - Kojić, Sanja
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2407
AB  - Changes in composition and new material characteristics require verification in clinical and experimental studies. Investigating glass-ionomer cements under laboratory conditions encounters problems in interpreting the results and in comparing them with other types of materials tested in the same way. As the connection between the glass-ionomer cements and the dental tissues is delicate, it is often the case that the impact of fractures and other artifacts is either underestimated or over-dimensioned when interpreting the results. A critical review was performed, with defining the main problems regarding the usage of SEM, EDX and nanoindentation techniques in glass-ionomer based materials evaluation.
T2  - Advanced Technologies & Materials
T1  - Challenges in experimental evaluation of morphological, chemo-mechanical and adhesive properties of glass-ionomer based dental materials
VL  - 44
IS  - 2
SP  - 25
EP  - 30
DO  - 10.24867/ATM-2019-2-005
ER  - 
@article{
author = "Petrović, Bojan and Marković, Evgenija and Perić, Tamara and Kojić, Sanja",
year = "2019",
abstract = "Changes in composition and new material characteristics require verification in clinical and experimental studies. Investigating glass-ionomer cements under laboratory conditions encounters problems in interpreting the results and in comparing them with other types of materials tested in the same way. As the connection between the glass-ionomer cements and the dental tissues is delicate, it is often the case that the impact of fractures and other artifacts is either underestimated or over-dimensioned when interpreting the results. A critical review was performed, with defining the main problems regarding the usage of SEM, EDX and nanoindentation techniques in glass-ionomer based materials evaluation.",
journal = "Advanced Technologies & Materials",
title = "Challenges in experimental evaluation of morphological, chemo-mechanical and adhesive properties of glass-ionomer based dental materials",
volume = "44",
number = "2",
pages = "25-30",
doi = "10.24867/ATM-2019-2-005"
}
Petrović, B., Marković, E., Perić, T.,& Kojić, S.. (2019). Challenges in experimental evaluation of morphological, chemo-mechanical and adhesive properties of glass-ionomer based dental materials. in Advanced Technologies & Materials, 44(2), 25-30.
https://doi.org/10.24867/ATM-2019-2-005
Petrović B, Marković E, Perić T, Kojić S. Challenges in experimental evaluation of morphological, chemo-mechanical and adhesive properties of glass-ionomer based dental materials. in Advanced Technologies & Materials. 2019;44(2):25-30.
doi:10.24867/ATM-2019-2-005 .
Petrović, Bojan, Marković, Evgenija, Perić, Tamara, Kojić, Sanja, "Challenges in experimental evaluation of morphological, chemo-mechanical and adhesive properties of glass-ionomer based dental materials" in Advanced Technologies & Materials, 44, no. 2 (2019):25-30,
https://doi.org/10.24867/ATM-2019-2-005 . .
1

Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition

Magić, Marko; Čolović, Božana; Jokanović, Vukoman; Vasilijić, Saša; Marković, Milan; Vučević, Dragana; Rudolf, Rebeka; Čolić, Snježana; Čolić, Miodrag

(Vojnomedicinska akademija - Institut za naučne informacije, Beograd, 2019)

TY  - JOUR
AU  - Magić, Marko
AU  - Čolović, Božana
AU  - Jokanović, Vukoman
AU  - Vasilijić, Saša
AU  - Marković, Milan
AU  - Vučević, Dragana
AU  - Rudolf, Rebeka
AU  - Čolić, Snježana
AU  - Čolić, Miodrag
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2445
AB  - Background/Aim. The deposition of hydroxyapatite (HAP) on the surface of titanium (Ti) alloys enhances bioactivity and osseointegration of the alloys which are widely used as implant materials in dentistry and orthopaedic surgery. However, the stability of HAP and subsequent biocompatibility of such alloys depends on the coating technique. The aim of this work was to test the cytotoxicity of a Ti alloy (Ti6Al4V), coated with HAP by a new plasma deposition method. Methods. The Ti6Al4V samples prepared as discs, 10 mm in diameter and 2 mm in thickness, were coated with HAP (one or both sides of the alloy) by an innovative atmospheric plasma jet method. The cytotoxicity of uncoated and HAP coated Ti6Al4V samples was evaluated by examining the morphological changes and viability of L929 fibroblasts in direct contact with the test materials. Adequate negative (polystyrene) and positive (nickel) control discs of the same size were used. The indirect cytotoxicity was determined by cultivating L929 cells with conditioning medium (CM), prepared as extract of the test samples incubated in the complete Roswell Park Memorial Institute (RPMI) 1640 medium for cell cultures. The cytotoxic effect was evaluated based on the degree of metabolic activity, necrosis, apoptosis and proliferation of L929 cells, using the appropriate assays. Results. Uncoated and one side HAP coated Ti6Al4V alloys were classified as non-cytotoxic according to the current ISO 10993-5 criteria, whereas two sides HAP coated Ti6Al4V alloy samples were slightly-moderate cytotoxic. The cytotoxicity manifested as the inhibition of metabolic activity and proliferation of L929 cells as well as the induction of their apoptosis and necrosis was significantly reduced by conditioning of HAP/Ti6Al4V alloys for 24 hours. The cytotoxic effect of HAP/Ti6Al4V CM only partly decreased in the presence of nifelate, a calcium (Ca) channel blocker, suggesting that Ca ions were not the only responsible cytotoxic agent. Conclusion. The original HAP coating procedure by atmospheric plasma spraying with high energy input enables the production of the stable adhesive coatings on Ti6Al4V alloys. Their cytotoxicity, which depends on the quantity of HAP coating layer, could be significantly reduced up to the non-cytotoxic level by prior conditioning of the alloys in culture medium. Such a procedure, which removes leachable toxic components, could be useful before implantation of HAP coated alloys in vivo.
AB  - Uvod/Cilj. Oblaganje površine legura titana (Ti) hidroksiapatitom (HAP) poboljšava bioaktivnost i oseointegraciju Ti legura, koje se široko koriste kao implantacioni materijali u stomatologiji i ortopediji. Međutim, stabilnost HAP prevlake i biokompatibilnost takvih legura zavise od primenjene tehnike oblaganja. Cilj ovog rada je bio da se ispita citotoksičnost Ti6Al4V legure obložene sa HAP pomoću plazme korišćenjem originalne metode. Metode. Uzorci Ti6Al4V legure u obliku diska, prečnika 10 mm, debljine 2 mm su presvučeni sa HAP (jednostrano ili obostrano) mlazom atmosferske plazme. Citotoksičnost neobložene i HAP-om obloženih Ti6Al4V legura je ispitivana na osnovu morfoloških karakteristika i vijabilnosti L929 fibroblasta u direktnom kontaktu ćelija sa test materijalima. Odgovarajuća negativna kontrola (polistirenski diskovi) i pozitivna kontrola (diskovi od nikla) istih veličina kao i diskovi Ti6Al4V legura su takođe uključeni u eksperimente. Indirektna citotoksičnost je procenjivana nakon kultivisanja L929 ćelija sa kondicioniranim medijumom (CM), koji je predstavljao ekstrakt testiranih uzoraka inkubiranih u kompletnom Roswel Park Memorial Institute (RPMI) 1640 medijumu za ćelijske kulture. Citotoksični efekat CM je procenjivan na osnovu stepena metaboličke aktivnosti, nekroze, apoptoze i proliferacije L929 ćelija, korišćenjem adekvatnih testova. Rezultati. Neobložena Ti6Al4V legura i Ti6Al4V legura obložena jednostrano sa HAP su okarakterisane kao necitotoksične na osnovu ISO 10993-5 kriterijuma, dok je Ti6Al4V legura obložena sa HAP obostrano pokazivala blagu do umerenu citotoksičnost. Citotoksičnost, koja se manifestovala smanjenjem metaboličke aktivnosti i proliferacije L929 ćelija kao i indukcijom njihove apoptoze i nekroze, je bila značajno smanjena ako su uzorci HAP-om presvučenih legura kondicionirani u medijumu u toku 24 časa. Citotoksičnost CM pripremljenih od Ti6Al4V legura obloženih sa HAP je bila samo delimično smanjena u prisustvu nifelata, blokatora kalcijumovih (Ca) kanala, što ukazuje da Ca joni nisu jedini citotoksični faktor. Zaključak. Originalna metoda oblaganja Ti6Al4V legure sa HAP pomoću atmosferske plazme u obliku spreja visoke energije omogućava stabilnu adheziju prevlake. Citotoksičnost ovako obrađene legure, koja zavisi od količine nanetog HAP, se može znatno smanjiti do necitotoksičnog nivoa prethodnim kondicioniranjem u medijumu. Ova procedura, kojom se uklanjaju rastvorljive toksične komponente, može biti korisna pre in vivo implantacije legura obloženih sa HAP.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition
T1  - Citotoksičnost legure titana obložene hidroksiapatitom pomoću mlaza plazme
VL  - 76
IS  - 5
SP  - 492
EP  - 501
DO  - 10.2298/VSP170809097M
ER  - 
@article{
author = "Magić, Marko and Čolović, Božana and Jokanović, Vukoman and Vasilijić, Saša and Marković, Milan and Vučević, Dragana and Rudolf, Rebeka and Čolić, Snježana and Čolić, Miodrag",
year = "2019",
abstract = "Background/Aim. The deposition of hydroxyapatite (HAP) on the surface of titanium (Ti) alloys enhances bioactivity and osseointegration of the alloys which are widely used as implant materials in dentistry and orthopaedic surgery. However, the stability of HAP and subsequent biocompatibility of such alloys depends on the coating technique. The aim of this work was to test the cytotoxicity of a Ti alloy (Ti6Al4V), coated with HAP by a new plasma deposition method. Methods. The Ti6Al4V samples prepared as discs, 10 mm in diameter and 2 mm in thickness, were coated with HAP (one or both sides of the alloy) by an innovative atmospheric plasma jet method. The cytotoxicity of uncoated and HAP coated Ti6Al4V samples was evaluated by examining the morphological changes and viability of L929 fibroblasts in direct contact with the test materials. Adequate negative (polystyrene) and positive (nickel) control discs of the same size were used. The indirect cytotoxicity was determined by cultivating L929 cells with conditioning medium (CM), prepared as extract of the test samples incubated in the complete Roswell Park Memorial Institute (RPMI) 1640 medium for cell cultures. The cytotoxic effect was evaluated based on the degree of metabolic activity, necrosis, apoptosis and proliferation of L929 cells, using the appropriate assays. Results. Uncoated and one side HAP coated Ti6Al4V alloys were classified as non-cytotoxic according to the current ISO 10993-5 criteria, whereas two sides HAP coated Ti6Al4V alloy samples were slightly-moderate cytotoxic. The cytotoxicity manifested as the inhibition of metabolic activity and proliferation of L929 cells as well as the induction of their apoptosis and necrosis was significantly reduced by conditioning of HAP/Ti6Al4V alloys for 24 hours. The cytotoxic effect of HAP/Ti6Al4V CM only partly decreased in the presence of nifelate, a calcium (Ca) channel blocker, suggesting that Ca ions were not the only responsible cytotoxic agent. Conclusion. The original HAP coating procedure by atmospheric plasma spraying with high energy input enables the production of the stable adhesive coatings on Ti6Al4V alloys. Their cytotoxicity, which depends on the quantity of HAP coating layer, could be significantly reduced up to the non-cytotoxic level by prior conditioning of the alloys in culture medium. Such a procedure, which removes leachable toxic components, could be useful before implantation of HAP coated alloys in vivo., Uvod/Cilj. Oblaganje površine legura titana (Ti) hidroksiapatitom (HAP) poboljšava bioaktivnost i oseointegraciju Ti legura, koje se široko koriste kao implantacioni materijali u stomatologiji i ortopediji. Međutim, stabilnost HAP prevlake i biokompatibilnost takvih legura zavise od primenjene tehnike oblaganja. Cilj ovog rada je bio da se ispita citotoksičnost Ti6Al4V legure obložene sa HAP pomoću plazme korišćenjem originalne metode. Metode. Uzorci Ti6Al4V legure u obliku diska, prečnika 10 mm, debljine 2 mm su presvučeni sa HAP (jednostrano ili obostrano) mlazom atmosferske plazme. Citotoksičnost neobložene i HAP-om obloženih Ti6Al4V legura je ispitivana na osnovu morfoloških karakteristika i vijabilnosti L929 fibroblasta u direktnom kontaktu ćelija sa test materijalima. Odgovarajuća negativna kontrola (polistirenski diskovi) i pozitivna kontrola (diskovi od nikla) istih veličina kao i diskovi Ti6Al4V legura su takođe uključeni u eksperimente. Indirektna citotoksičnost je procenjivana nakon kultivisanja L929 ćelija sa kondicioniranim medijumom (CM), koji je predstavljao ekstrakt testiranih uzoraka inkubiranih u kompletnom Roswel Park Memorial Institute (RPMI) 1640 medijumu za ćelijske kulture. Citotoksični efekat CM je procenjivan na osnovu stepena metaboličke aktivnosti, nekroze, apoptoze i proliferacije L929 ćelija, korišćenjem adekvatnih testova. Rezultati. Neobložena Ti6Al4V legura i Ti6Al4V legura obložena jednostrano sa HAP su okarakterisane kao necitotoksične na osnovu ISO 10993-5 kriterijuma, dok je Ti6Al4V legura obložena sa HAP obostrano pokazivala blagu do umerenu citotoksičnost. Citotoksičnost, koja se manifestovala smanjenjem metaboličke aktivnosti i proliferacije L929 ćelija kao i indukcijom njihove apoptoze i nekroze, je bila značajno smanjena ako su uzorci HAP-om presvučenih legura kondicionirani u medijumu u toku 24 časa. Citotoksičnost CM pripremljenih od Ti6Al4V legura obloženih sa HAP je bila samo delimično smanjena u prisustvu nifelata, blokatora kalcijumovih (Ca) kanala, što ukazuje da Ca joni nisu jedini citotoksični faktor. Zaključak. Originalna metoda oblaganja Ti6Al4V legure sa HAP pomoću atmosferske plazme u obliku spreja visoke energije omogućava stabilnu adheziju prevlake. Citotoksičnost ovako obrađene legure, koja zavisi od količine nanetog HAP, se može znatno smanjiti do necitotoksičnog nivoa prethodnim kondicioniranjem u medijumu. Ova procedura, kojom se uklanjaju rastvorljive toksične komponente, može biti korisna pre in vivo implantacije legura obloženih sa HAP.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition, Citotoksičnost legure titana obložene hidroksiapatitom pomoću mlaza plazme",
volume = "76",
number = "5",
pages = "492-501",
doi = "10.2298/VSP170809097M"
}
Magić, M., Čolović, B., Jokanović, V., Vasilijić, S., Marković, M., Vučević, D., Rudolf, R., Čolić, S.,& Čolić, M.. (2019). Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 76(5), 492-501.
https://doi.org/10.2298/VSP170809097M
Magić M, Čolović B, Jokanović V, Vasilijić S, Marković M, Vučević D, Rudolf R, Čolić S, Čolić M. Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition. in Vojnosanitetski pregled. 2019;76(5):492-501.
doi:10.2298/VSP170809097M .
Magić, Marko, Čolović, Božana, Jokanović, Vukoman, Vasilijić, Saša, Marković, Milan, Vučević, Dragana, Rudolf, Rebeka, Čolić, Snježana, Čolić, Miodrag, "Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition" in Vojnosanitetski pregled, 76, no. 5 (2019):492-501,
https://doi.org/10.2298/VSP170809097M . .

Influence of photodynamic therapy and diode laser on successful endodontic treatment of young permanent teeth and biostimulation of stem cells from apical papilla

Trišić, Dijana

(Univerzitet u Beogradu, Stomatološki fakultet, 2019)

TY  - THES
AU  - Trišić, Dijana
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7249
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:20993/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51890703
UR  - http://nardus.mpn.gov.rs/123456789/12096
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1041
AB  - Stem cells from apical papilla (SCAPs) represent a homogeneous cell population which differentiates into primary odontoblasts and form primary root dentine. Young permanent teeth, more prone to dental trauma and developmental anomalies, have greater susceptibility to develop endodontic infections. During endodontic treatment of young permanent teeth, control of infection represents a necessary step in creating conditions for further tissue regeneration and healing. Due to good antibacterial potential, the application of photodynamic therapy and a high-power diode laser has been recommended, as addition to standard endodontic protocol. The Aim of this study was to investigate biocompatibility of diode laser and photodynamic therapy on stem cells from apical papilla, as well as the antibacterial effect of standard disinfection, lasers, and materials for definitive root canal obturation in endodontic treatment of young permanent teeth. Materials and methods: SCAPs were isolated and characterized by internationally accepted criteria for mesenchymal stem cells. Biocompatibility of diode laser and photodynamic therapy was investigated on characterized stem cells by the mitochondrial activity assay (MTT assay) and cells viability assay (Neutral red assay) after 1 hour, 1 and 7 days. Lasers bbiostimulation effect on cells migration was evaluated by scratch assay, while the effect on osteogenic differentiation was investigated by measurement of alkaline phosphatase (3 7, and 14 days) and Alizarin red staining. Relative gene expression analysis and protein staining of selected markers for odonto/osteogenic differentiation, have been performed as well after 21 days. In a randomized controlled clinical study, patients have been relocated in 3 study groups. Antibacterial effect was evaluated after standard endodontic treatment, as well as after diode laser application and photodynamic therapy, in corresponding groups. Samples were seeded on microbial plates, and additionally analyzed by molecular methods. Antibacterial effects of calcium hydroxide, mineral trioxide aggregate, and calcium silicate with the addition of bismuth oxide were investigated on clinical isolates, by the method of double agar diffusion test in three time periods (1, 3, and 24 hours)...
AB  - Uvod: Matične ćelije poreklom iz apikalne papile (SCAP) predstavljaju homogenu ćelijsku populaciju koja diferentuje u primarne odontoblaste i formira primarni dentin korena. Mladi stalni zubi, usled sklonosti ka povredama i razvojnim anomalijama, su u velikom riziku za nastanak endodontskih oboljenja. Tokom endodontskog lečenja mladih stalnih zuba kontrola infekcije predstavlja neophodan korak u stvaranju uslova za regeneraciju i zarastanje. Usled dobrog antibakterijskog potencijala, predložena je upotreba fotodinamske terapije i diodnih lasera velike snage u endodontskom lečenju, kao dodatak važećim protokolima. Cilj ovog istraživanja bio je da se ispita biokompatibilnost diodnog lasera i fotodinamske terapije na matičnim ćelijama poreklom iz apikalne papile zuba, kao i antimikrobno dejstvo tradicionalnih antimikrobnih sredstava, lasera, i materijala za definitivnu opturaciju kanala korena u endodontskom lečenju mladih stalnih zuba. Materijali i metode: Ćelije iz apikalne papile su izolovane i okarakterisane prema međunarodno definisanim kriterijumima za mezenhimalne matične ćelije. Na okarakterisanim matičnim ćelijama poreklom iz apikalne papile ispitana je biokompatibilnost diodnog lasera i fotodinamske terapije testom mitohondrijske aktivnosti (MTT) i direktnim testom vijabilnosti ćelija (Neutralno crveno) nakon 1 sata, 1 i 7 dana. Biostimulativno dejstvo lasera na potencijal migracije ispitano je testom ogrebotine, dok je uticaj na potencijal osteogenog usmeravanja ispitana merenjem alkalne fosfataze (3, 7 i 14 dana) i bojenjem Alizarin crvenim. Ispitan je i nivo relativne genske ekspresije i specifično bojenje proteina za markere odonto/osteogene diferencijacije, nakon 21 dana. U randomizovanoj kontrolisanoj kliničkoj studiji, pacijenti su raspoređeni u 3 ogledne grupe. Antimikrobno dejstvo je ispitano nakon dejstva standardnih antimikrobnih sredstava, kao i nakon dodatnog dejstva diodnog lasera i fotodinamske terapije u odgovarajućim grupama...
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - Influence of photodynamic therapy and diode laser on successful endodontic treatment of young permanent teeth and biostimulation of stem cells from apical papilla
T1  - Uticaj fotodinamske terapije i diodnog lasera na uspešnost endodontskog lečenja mladih stalnih zuba i biostimulaciju matičnih ćelija poreklom iz apikalne papile
UR  - https://hdl.handle.net/21.15107/rcub_nardus_12096
ER  - 
@phdthesis{
author = "Trišić, Dijana",
year = "2019",
abstract = "Stem cells from apical papilla (SCAPs) represent a homogeneous cell population which differentiates into primary odontoblasts and form primary root dentine. Young permanent teeth, more prone to dental trauma and developmental anomalies, have greater susceptibility to develop endodontic infections. During endodontic treatment of young permanent teeth, control of infection represents a necessary step in creating conditions for further tissue regeneration and healing. Due to good antibacterial potential, the application of photodynamic therapy and a high-power diode laser has been recommended, as addition to standard endodontic protocol. The Aim of this study was to investigate biocompatibility of diode laser and photodynamic therapy on stem cells from apical papilla, as well as the antibacterial effect of standard disinfection, lasers, and materials for definitive root canal obturation in endodontic treatment of young permanent teeth. Materials and methods: SCAPs were isolated and characterized by internationally accepted criteria for mesenchymal stem cells. Biocompatibility of diode laser and photodynamic therapy was investigated on characterized stem cells by the mitochondrial activity assay (MTT assay) and cells viability assay (Neutral red assay) after 1 hour, 1 and 7 days. Lasers bbiostimulation effect on cells migration was evaluated by scratch assay, while the effect on osteogenic differentiation was investigated by measurement of alkaline phosphatase (3 7, and 14 days) and Alizarin red staining. Relative gene expression analysis and protein staining of selected markers for odonto/osteogenic differentiation, have been performed as well after 21 days. In a randomized controlled clinical study, patients have been relocated in 3 study groups. Antibacterial effect was evaluated after standard endodontic treatment, as well as after diode laser application and photodynamic therapy, in corresponding groups. Samples were seeded on microbial plates, and additionally analyzed by molecular methods. Antibacterial effects of calcium hydroxide, mineral trioxide aggregate, and calcium silicate with the addition of bismuth oxide were investigated on clinical isolates, by the method of double agar diffusion test in three time periods (1, 3, and 24 hours)..., Uvod: Matične ćelije poreklom iz apikalne papile (SCAP) predstavljaju homogenu ćelijsku populaciju koja diferentuje u primarne odontoblaste i formira primarni dentin korena. Mladi stalni zubi, usled sklonosti ka povredama i razvojnim anomalijama, su u velikom riziku za nastanak endodontskih oboljenja. Tokom endodontskog lečenja mladih stalnih zuba kontrola infekcije predstavlja neophodan korak u stvaranju uslova za regeneraciju i zarastanje. Usled dobrog antibakterijskog potencijala, predložena je upotreba fotodinamske terapije i diodnih lasera velike snage u endodontskom lečenju, kao dodatak važećim protokolima. Cilj ovog istraživanja bio je da se ispita biokompatibilnost diodnog lasera i fotodinamske terapije na matičnim ćelijama poreklom iz apikalne papile zuba, kao i antimikrobno dejstvo tradicionalnih antimikrobnih sredstava, lasera, i materijala za definitivnu opturaciju kanala korena u endodontskom lečenju mladih stalnih zuba. Materijali i metode: Ćelije iz apikalne papile su izolovane i okarakterisane prema međunarodno definisanim kriterijumima za mezenhimalne matične ćelije. Na okarakterisanim matičnim ćelijama poreklom iz apikalne papile ispitana je biokompatibilnost diodnog lasera i fotodinamske terapije testom mitohondrijske aktivnosti (MTT) i direktnim testom vijabilnosti ćelija (Neutralno crveno) nakon 1 sata, 1 i 7 dana. Biostimulativno dejstvo lasera na potencijal migracije ispitano je testom ogrebotine, dok je uticaj na potencijal osteogenog usmeravanja ispitana merenjem alkalne fosfataze (3, 7 i 14 dana) i bojenjem Alizarin crvenim. Ispitan je i nivo relativne genske ekspresije i specifično bojenje proteina za markere odonto/osteogene diferencijacije, nakon 21 dana. U randomizovanoj kontrolisanoj kliničkoj studiji, pacijenti su raspoređeni u 3 ogledne grupe. Antimikrobno dejstvo je ispitano nakon dejstva standardnih antimikrobnih sredstava, kao i nakon dodatnog dejstva diodnog lasera i fotodinamske terapije u odgovarajućim grupama...",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "Influence of photodynamic therapy and diode laser on successful endodontic treatment of young permanent teeth and biostimulation of stem cells from apical papilla, Uticaj fotodinamske terapije i diodnog lasera na uspešnost endodontskog lečenja mladih stalnih zuba i biostimulaciju matičnih ćelija poreklom iz apikalne papile",
url = "https://hdl.handle.net/21.15107/rcub_nardus_12096"
}
Trišić, D.. (2019). Influence of photodynamic therapy and diode laser on successful endodontic treatment of young permanent teeth and biostimulation of stem cells from apical papilla. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_12096
Trišić D. Influence of photodynamic therapy and diode laser on successful endodontic treatment of young permanent teeth and biostimulation of stem cells from apical papilla. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_12096 .
Trišić, Dijana, "Influence of photodynamic therapy and diode laser on successful endodontic treatment of young permanent teeth and biostimulation of stem cells from apical papilla" (2019),
https://hdl.handle.net/21.15107/rcub_nardus_12096 .

A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis

Čolović, Božana; Janković, Ognjenka; Živković, Slavoljub; Zizak, Zeljko; Zizak, Irina Besu; Jokanović, Vukoman

(Elsevier Sci Ltd, Oxford, 2019)

TY  - JOUR
AU  - Čolović, Božana
AU  - Janković, Ognjenka
AU  - Živković, Slavoljub
AU  - Zizak, Zeljko
AU  - Zizak, Irina Besu
AU  - Jokanović, Vukoman
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2442
AB  - The synthesis of a new endodontic mixture based on calcium aluminate cement is reported. Physico-chemical and mechanical properties of the mixture were investigated before and after various hydration periods. Phase analysis of the mixture was performed using X-ray diffraction (XRD), while morphological analysis was performed by scanning electron microscopy (SEM) equipped with EDS which revealed elemental composition of the mixture sample. Investigations of mechanical properties showed that this new endodontic mixture possessed good mechanical properties (compressive strength after 28 days aging was 94 MPa) and relatively short setting time (2 h). Additionally, cytotoxicity of the mixture was investigated by MTT assay, while genotoxicity was investigated by Comet assay, using MRC-5 cell line. The obtained results make this material very promising for potential application in dental practice.
PB  - Elsevier Sci Ltd, Oxford
T2  - Ceramics International
T1  - A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis
VL  - 45
IS  - 7
SP  - 9211
EP  - 9218
DO  - 10.1016/j.ceramint.2019.01.266
ER  - 
@article{
author = "Čolović, Božana and Janković, Ognjenka and Živković, Slavoljub and Zizak, Zeljko and Zizak, Irina Besu and Jokanović, Vukoman",
year = "2019",
abstract = "The synthesis of a new endodontic mixture based on calcium aluminate cement is reported. Physico-chemical and mechanical properties of the mixture were investigated before and after various hydration periods. Phase analysis of the mixture was performed using X-ray diffraction (XRD), while morphological analysis was performed by scanning electron microscopy (SEM) equipped with EDS which revealed elemental composition of the mixture sample. Investigations of mechanical properties showed that this new endodontic mixture possessed good mechanical properties (compressive strength after 28 days aging was 94 MPa) and relatively short setting time (2 h). Additionally, cytotoxicity of the mixture was investigated by MTT assay, while genotoxicity was investigated by Comet assay, using MRC-5 cell line. The obtained results make this material very promising for potential application in dental practice.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Ceramics International",
title = "A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis",
volume = "45",
number = "7",
pages = "9211-9218",
doi = "10.1016/j.ceramint.2019.01.266"
}
Čolović, B., Janković, O., Živković, S., Zizak, Z., Zizak, I. B.,& Jokanović, V.. (2019). A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis. in Ceramics International
Elsevier Sci Ltd, Oxford., 45(7), 9211-9218.
https://doi.org/10.1016/j.ceramint.2019.01.266
Čolović B, Janković O, Živković S, Zizak Z, Zizak IB, Jokanović V. A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis. in Ceramics International. 2019;45(7):9211-9218.
doi:10.1016/j.ceramint.2019.01.266 .
Čolović, Božana, Janković, Ognjenka, Živković, Slavoljub, Zizak, Zeljko, Zizak, Irina Besu, Jokanović, Vukoman, "A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis" in Ceramics International, 45, no. 7 (2019):9211-9218,
https://doi.org/10.1016/j.ceramint.2019.01.266 . .
3
2
3

Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics

Jokanović, Vukoman; Čolović, Božana; Ćetenović, Bojana; Živković, Slavoljub

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2019)

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Ćetenović, Bojana
AU  - Živković, Slavoljub
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2427
AB  - Various magnetron-sputtering methods for surface modification of shape memory alloys (SMA) are described in this paper. These methods belong to the most effective methods, which enable mechanical reinforcing of the SMA, showing numerous advantages over conventional methods of electro-polishing. In addition, surface modified SMA, particularly with equiatomic TiNi coatings, is crucial for further development of various endodontic instruments; wires and brackets used for orthodontic teeth movements. Active coatings with bactericide properties and coatings that can be used as barrier for release of toxic Ni ions from the bulk body of SMA obtained by various magnetron-sputtering methods can be successfully combined. Therefore, the review of these methods is given in this paper, with their main characteristics and drawbacks. Magnetron sputtering deposition involves surface modification of SMA in a single-layer, multilayer, graded layers, and nanocomposite thin coatings for obtaining systems with superior "functional" characteristics. These are hardness, scratch, abrasion, and erosion resistance, improved adhesion to various technologically important substrate materials such as polymers, hydrophobicity or hydrophilicity, long-term chemical, thermal, and environmental stability, gas and vapor impermeability, and others. This paper is critical review of the advances in the development of magnetron sputtering modified SMA products in dentistry, with in advance predictable physicochemical, structural and antimicrobial properties.
AB  - U ovom radu su opisane različite metode magnetronskog raspršivanja za površinsku modifikaciju memorijskih legura (shape memory alloys - SMA). Ove metode spadaju u najefikasnije metode koje omogućavaju mehaničko ojačavanje SMA, pokazujući brojne prednosti u odnosu na konvencionalne metode elektropoliranja, koje su najčešće korišćene u savremenoj ortodontskoj i endodontskoj praksi. Pored toga, površinski modifikovane SMA, posebno sa ekviatomskim Ti i Ni udelima unutar prevlake, presudne su za dalji razvoj različitih endodontskih instrumenata, žica i konzola koje se koriste za ortodontska pomeranja zuba. Aktivne prevlake sa baktericidnim svojstvima i prevlake koje služe kao barijere protiv otpuštanja toksičnih Ni jona iz SMA unutar organizma mogu biti dobijene različitim metodama magnetronskog raspršivanja, pri čemu se različite varijante ove metode mogu uspešno kombinovati. Zbog svega toga u ovom radu je dat pregled ovih metoda, sa njihovim glavnim karakteristikama i nedostacima. Magnetronsko nanošenje raspršivanjem uključuje površinsku modifikaciju SMA u jednoslojnim, višeslojnim, gradiranim slojevima i nanokompozitne tanke prevlake za dobijanje sistema sa superiornim "funkcionalnim" karakteristikama, kao što su vrlo visoka tvrdoća, otpornost na habanje, abraziju i eroziju, poboljšano prijanjanje na različite tehnološki važne supstratne materijale kao što su polimeri, hidrofobnost ili hidrofilnost, dugotrajna hemijska, termička i ekološka stabilnost, nepropusnost gasa i para i drugi. Ovaj rad predstavlja neku vrstu kritičkog pregleda napretka u razvoju magnetronskog raspršivanja modifikovanih SMA proizvoda u stomatologiji, sa unapred predvidljivim fizičko-hemijskim, strukturnim i antimikrobnim osobinama.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics
T1  - Metode magnetronskog raspršivanja za površinsku modifikaciju memorijskih legura za primenu u ortodonciji i endodonciji
VL  - 66
IS  - 2
SP  - 87
EP  - 96
DO  - 10.2478/sdj-2019-0010
ER  - 
@article{
author = "Jokanović, Vukoman and Čolović, Božana and Ćetenović, Bojana and Živković, Slavoljub",
year = "2019",
abstract = "Various magnetron-sputtering methods for surface modification of shape memory alloys (SMA) are described in this paper. These methods belong to the most effective methods, which enable mechanical reinforcing of the SMA, showing numerous advantages over conventional methods of electro-polishing. In addition, surface modified SMA, particularly with equiatomic TiNi coatings, is crucial for further development of various endodontic instruments; wires and brackets used for orthodontic teeth movements. Active coatings with bactericide properties and coatings that can be used as barrier for release of toxic Ni ions from the bulk body of SMA obtained by various magnetron-sputtering methods can be successfully combined. Therefore, the review of these methods is given in this paper, with their main characteristics and drawbacks. Magnetron sputtering deposition involves surface modification of SMA in a single-layer, multilayer, graded layers, and nanocomposite thin coatings for obtaining systems with superior "functional" characteristics. These are hardness, scratch, abrasion, and erosion resistance, improved adhesion to various technologically important substrate materials such as polymers, hydrophobicity or hydrophilicity, long-term chemical, thermal, and environmental stability, gas and vapor impermeability, and others. This paper is critical review of the advances in the development of magnetron sputtering modified SMA products in dentistry, with in advance predictable physicochemical, structural and antimicrobial properties., U ovom radu su opisane različite metode magnetronskog raspršivanja za površinsku modifikaciju memorijskih legura (shape memory alloys - SMA). Ove metode spadaju u najefikasnije metode koje omogućavaju mehaničko ojačavanje SMA, pokazujući brojne prednosti u odnosu na konvencionalne metode elektropoliranja, koje su najčešće korišćene u savremenoj ortodontskoj i endodontskoj praksi. Pored toga, površinski modifikovane SMA, posebno sa ekviatomskim Ti i Ni udelima unutar prevlake, presudne su za dalji razvoj različitih endodontskih instrumenata, žica i konzola koje se koriste za ortodontska pomeranja zuba. Aktivne prevlake sa baktericidnim svojstvima i prevlake koje služe kao barijere protiv otpuštanja toksičnih Ni jona iz SMA unutar organizma mogu biti dobijene različitim metodama magnetronskog raspršivanja, pri čemu se različite varijante ove metode mogu uspešno kombinovati. Zbog svega toga u ovom radu je dat pregled ovih metoda, sa njihovim glavnim karakteristikama i nedostacima. Magnetronsko nanošenje raspršivanjem uključuje površinsku modifikaciju SMA u jednoslojnim, višeslojnim, gradiranim slojevima i nanokompozitne tanke prevlake za dobijanje sistema sa superiornim "funkcionalnim" karakteristikama, kao što su vrlo visoka tvrdoća, otpornost na habanje, abraziju i eroziju, poboljšano prijanjanje na različite tehnološki važne supstratne materijale kao što su polimeri, hidrofobnost ili hidrofilnost, dugotrajna hemijska, termička i ekološka stabilnost, nepropusnost gasa i para i drugi. Ovaj rad predstavlja neku vrstu kritičkog pregleda napretka u razvoju magnetronskog raspršivanja modifikovanih SMA proizvoda u stomatologiji, sa unapred predvidljivim fizičko-hemijskim, strukturnim i antimikrobnim osobinama.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics, Metode magnetronskog raspršivanja za površinsku modifikaciju memorijskih legura za primenu u ortodonciji i endodonciji",
volume = "66",
number = "2",
pages = "87-96",
doi = "10.2478/sdj-2019-0010"
}
Jokanović, V., Čolović, B., Ćetenović, B.,& Živković, S.. (2019). Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 66(2), 87-96.
https://doi.org/10.2478/sdj-2019-0010
Jokanović V, Čolović B, Ćetenović B, Živković S. Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics. in Stomatološki glasnik Srbije. 2019;66(2):87-96.
doi:10.2478/sdj-2019-0010 .
Jokanović, Vukoman, Čolović, Božana, Ćetenović, Bojana, Živković, Slavoljub, "Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics" in Stomatološki glasnik Srbije, 66, no. 2 (2019):87-96,
https://doi.org/10.2478/sdj-2019-0010 . .

Antibacterial effects of new endodontic materials based on calcium silicates

Trišić, Dijana; Ćetenović, Bojana; Zdravković, Nemanja; Marković, Tatjana; Dojčinović, Biljana; Jokanović, Vukoman; Marković, Dejan

(Vojnomedicinska akademija - Institut za naučne informacije, Beograd, 2019)

TY  - JOUR
AU  - Trišić, Dijana
AU  - Ćetenović, Bojana
AU  - Zdravković, Nemanja
AU  - Marković, Tatjana
AU  - Dojčinović, Biljana
AU  - Jokanović, Vukoman
AU  - Marković, Dejan
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2444
AB  - Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation. The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA +) and UltraCal XS (CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Entero-coccus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the an-tibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA + had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA + in 3h and 24h of the incubation period (p  lt  0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA +. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTA substitution.
AB  - Uvod/Cilj. Osnovni cilj endodonskog lečenja je eliminacija patološki izmenjenog tkiva, eliminacija infekcije korensko kanala i njegovo hermetičko trodimenzionalno zatvaranje. Cilj istraživanja je bio da se proceni antibakterijska aktivnost novih endodontskih nano-strukturiranih materijala na bazi visoko aktivnih kalcijum silikata albo-mineral polyoxide carbonate aggregate (ALBO-MPCA) i calcium silicates (CS) u odnosu na mineral trioxide aggregate (MTA +) i UltraCal XS (CH). Metode. Testirana je antibakterijska aktivnost materijala protiv Staphylococcus aureus (ATCC 25923) i Enterococcus faecalis (ATCC 14506), kao i kliničkih izolata: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus i Streptococcus vestibularis pomoću agar difuzionog testa. Merenja pH vred-nosti obavljena su korišćenjem pH metra. Ukupan iznos oslobođenih jona određivan je pomoću ICP-OES. Rezultati. Svi testirani materijali pokazali su najbolji antibakterijski efekat nakon 1 h od inkubacije. Nakon 3 h i 24 h od inkubacije, antibakterijski efekat svih testiranih materijala bio je sličan. Agar difuzioni test pokazao je da materijali ALBOMPCA, CS i MTA+ ispoljavaju slične zone inhibicije rasta (p > 0.05) osim u slučaju Staphylococcus aureus, gde je materijal ALBO-MPCA pokazao bolje antibakterijsko dejstvo nego MTA+ nakon 3 h i 24 h od inkubacije (p  lt  0.05). Nakon 24 h od inkubacije, zone inhibicije su bile najizraženije u slučaju materijala CS protiv Staphylococcus aureus (16.67 ± 2.34 mm), zatim ALBO-MPCA (14.67 ± 1.21 mm), a najslabije u slučaju CS protiv Enterococcus faecalis (6.50±1.76 mm). Materijal CH ispoljio je najveću pH vrednost, zatim ALBOMPCA, CS i MTA+. Zaključak. Ispoljeni antibakterijski efekti ukazuju na to da materijali na bazi visoko aktivnih kalcijum silikata mogu da predstavljaju efikasnu zamenu za MTA u terapiji zuba sa nezavršenim rastom korena u jednoj poseti, te se stoga preporučuju za dalja klinička ispitivanja
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Antibacterial effects of new endodontic materials based on calcium silicates
T1  - Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata
VL  - 76
IS  - 4
SP  - 365
EP  - 372
DO  - 10.2298/VSP161231130T
ER  - 
@article{
author = "Trišić, Dijana and Ćetenović, Bojana and Zdravković, Nemanja and Marković, Tatjana and Dojčinović, Biljana and Jokanović, Vukoman and Marković, Dejan",
year = "2019",
abstract = "Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation. The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA +) and UltraCal XS (CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Entero-coccus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the an-tibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA + had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA + in 3h and 24h of the incubation period (p  lt  0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA +. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTA substitution., Uvod/Cilj. Osnovni cilj endodonskog lečenja je eliminacija patološki izmenjenog tkiva, eliminacija infekcije korensko kanala i njegovo hermetičko trodimenzionalno zatvaranje. Cilj istraživanja je bio da se proceni antibakterijska aktivnost novih endodontskih nano-strukturiranih materijala na bazi visoko aktivnih kalcijum silikata albo-mineral polyoxide carbonate aggregate (ALBO-MPCA) i calcium silicates (CS) u odnosu na mineral trioxide aggregate (MTA +) i UltraCal XS (CH). Metode. Testirana je antibakterijska aktivnost materijala protiv Staphylococcus aureus (ATCC 25923) i Enterococcus faecalis (ATCC 14506), kao i kliničkih izolata: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus i Streptococcus vestibularis pomoću agar difuzionog testa. Merenja pH vred-nosti obavljena su korišćenjem pH metra. Ukupan iznos oslobođenih jona određivan je pomoću ICP-OES. Rezultati. Svi testirani materijali pokazali su najbolji antibakterijski efekat nakon 1 h od inkubacije. Nakon 3 h i 24 h od inkubacije, antibakterijski efekat svih testiranih materijala bio je sličan. Agar difuzioni test pokazao je da materijali ALBOMPCA, CS i MTA+ ispoljavaju slične zone inhibicije rasta (p > 0.05) osim u slučaju Staphylococcus aureus, gde je materijal ALBO-MPCA pokazao bolje antibakterijsko dejstvo nego MTA+ nakon 3 h i 24 h od inkubacije (p  lt  0.05). Nakon 24 h od inkubacije, zone inhibicije su bile najizraženije u slučaju materijala CS protiv Staphylococcus aureus (16.67 ± 2.34 mm), zatim ALBO-MPCA (14.67 ± 1.21 mm), a najslabije u slučaju CS protiv Enterococcus faecalis (6.50±1.76 mm). Materijal CH ispoljio je najveću pH vrednost, zatim ALBOMPCA, CS i MTA+. Zaključak. Ispoljeni antibakterijski efekti ukazuju na to da materijali na bazi visoko aktivnih kalcijum silikata mogu da predstavljaju efikasnu zamenu za MTA u terapiji zuba sa nezavršenim rastom korena u jednoj poseti, te se stoga preporučuju za dalja klinička ispitivanja",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Antibacterial effects of new endodontic materials based on calcium silicates, Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata",
volume = "76",
number = "4",
pages = "365-372",
doi = "10.2298/VSP161231130T"
}
Trišić, D., Ćetenović, B., Zdravković, N., Marković, T., Dojčinović, B., Jokanović, V.,& Marković, D.. (2019). Antibacterial effects of new endodontic materials based on calcium silicates. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 76(4), 365-372.
https://doi.org/10.2298/VSP161231130T
Trišić D, Ćetenović B, Zdravković N, Marković T, Dojčinović B, Jokanović V, Marković D. Antibacterial effects of new endodontic materials based on calcium silicates. in Vojnosanitetski pregled. 2019;76(4):365-372.
doi:10.2298/VSP161231130T .
Trišić, Dijana, Ćetenović, Bojana, Zdravković, Nemanja, Marković, Tatjana, Dojčinović, Biljana, Jokanović, Vukoman, Marković, Dejan, "Antibacterial effects of new endodontic materials based on calcium silicates" in Vojnosanitetski pregled, 76, no. 4 (2019):365-372,
https://doi.org/10.2298/VSP161231130T . .
1

Physico-chemical and biological properties of dental calcium silicate cements - literature review

Ilić, Dragan; Antonijević, Đorđe; Biočanin, Vladimir; Čolović, Božana; Danilović, Vesna; Komlev, Vladimir S.; Teterina, Anastasia; Jokanović, Vukoman

(Savez hemijskih inženjera, Beograd, 2019)

TY  - JOUR
AU  - Ilić, Dragan
AU  - Antonijević, Đorđe
AU  - Biočanin, Vladimir
AU  - Čolović, Božana
AU  - Danilović, Vesna
AU  - Komlev, Vladimir S.
AU  - Teterina, Anastasia
AU  - Jokanović, Vukoman
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2411
AB  - Dental cement materials have been developed with the aim to replace hard dental tissues. The first material used for pulp capping, root canal obturation, bifurcation perforation and apexification is calcium hydroxide (in 1920). A half century later, glass-ionomer cements began to suppress it as dentine substitutes. Finally, in the 1990s, calcium silicate (CS) material appeared in the dental research community as the most promising dentine substitute capable to adequately meet all clinical requirements. The aim of this paper is to present an overview of literature related to studies about CS materials taking into account their physical, chemical and biological properties and clinical applications. This review aims to discuss beneficial and adverse characteristics of CSs concerning interactions to the hard dentine and soft pulp/periodontal tissues. This review article deals with the literature data about currently commercially available CS concerning laboratory and clinical findings. 109 scientific articles were analyzed of which 62 references reported in vitro and 26 in vivo investigations while 21 references comprised reports, reviews and books dealing with both, in vitro and in vivo investigations. Although further data collection is necessary, CSs are promising materials that represent a gold standard for numerous dental clinical procedures.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska Industrija
T1  - Physico-chemical and biological properties of dental calcium silicate cements - literature review
VL  - 73
IS  - 5
SP  - 281
EP  - 294
DO  - 10.2298/HEMIND190614027I
ER  - 
@article{
author = "Ilić, Dragan and Antonijević, Đorđe and Biočanin, Vladimir and Čolović, Božana and Danilović, Vesna and Komlev, Vladimir S. and Teterina, Anastasia and Jokanović, Vukoman",
year = "2019",
abstract = "Dental cement materials have been developed with the aim to replace hard dental tissues. The first material used for pulp capping, root canal obturation, bifurcation perforation and apexification is calcium hydroxide (in 1920). A half century later, glass-ionomer cements began to suppress it as dentine substitutes. Finally, in the 1990s, calcium silicate (CS) material appeared in the dental research community as the most promising dentine substitute capable to adequately meet all clinical requirements. The aim of this paper is to present an overview of literature related to studies about CS materials taking into account their physical, chemical and biological properties and clinical applications. This review aims to discuss beneficial and adverse characteristics of CSs concerning interactions to the hard dentine and soft pulp/periodontal tissues. This review article deals with the literature data about currently commercially available CS concerning laboratory and clinical findings. 109 scientific articles were analyzed of which 62 references reported in vitro and 26 in vivo investigations while 21 references comprised reports, reviews and books dealing with both, in vitro and in vivo investigations. Although further data collection is necessary, CSs are promising materials that represent a gold standard for numerous dental clinical procedures.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska Industrija",
title = "Physico-chemical and biological properties of dental calcium silicate cements - literature review",
volume = "73",
number = "5",
pages = "281-294",
doi = "10.2298/HEMIND190614027I"
}
Ilić, D., Antonijević, Đ., Biočanin, V., Čolović, B., Danilović, V., Komlev, V. S., Teterina, A.,& Jokanović, V.. (2019). Physico-chemical and biological properties of dental calcium silicate cements - literature review. in Hemijska Industrija
Savez hemijskih inženjera, Beograd., 73(5), 281-294.
https://doi.org/10.2298/HEMIND190614027I
Ilić D, Antonijević Đ, Biočanin V, Čolović B, Danilović V, Komlev VS, Teterina A, Jokanović V. Physico-chemical and biological properties of dental calcium silicate cements - literature review. in Hemijska Industrija. 2019;73(5):281-294.
doi:10.2298/HEMIND190614027I .
Ilić, Dragan, Antonijević, Đorđe, Biočanin, Vladimir, Čolović, Božana, Danilović, Vesna, Komlev, Vladimir S., Teterina, Anastasia, Jokanović, Vukoman, "Physico-chemical and biological properties of dental calcium silicate cements - literature review" in Hemijska Industrija, 73, no. 5 (2019):281-294,
https://doi.org/10.2298/HEMIND190614027I . .
2
1
4

Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis

Paras, S.; Janković, Ognjenka; Trišić, Dijana; Čolović, Božana; Mitrović-Ajtić, O.; Dekić, R.; Soldatović, Ivan; Živković-Sandić, Marija; Živković, Slavoljub; Jokanović, Vukoman

(Wiley, Hoboken, 2019)

TY  - JOUR
AU  - Paras, S.
AU  - Janković, Ognjenka
AU  - Trišić, Dijana
AU  - Čolović, Božana
AU  - Mitrović-Ajtić, O.
AU  - Dekić, R.
AU  - Soldatović, Ivan
AU  - Živković-Sandić, Marija
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2393
AB  - Aim To examine the potential systemic toxicity of nanostructured materials based on calcium silicate and calcium aluminate, for potential application in Dentistry. Methodology Twenty-four Albino Wistar rats aged 2 months were used as an in vivo animal model for subcutaneous implantation of the investigated materials, placed in polyethylene tubes. Thirty days after implantation, the livers of the rats were analysed and following histological and stereological parameters were evaluated for volume density of hepatocytes and blood sinusoids, number and numerical density of hepatocytes, surface of hepatocytes and their nucleuses, nucleocytoplasmic ratio and mitotic index of hepatocytes. Stereological measurements were achieved using Cavalieri's principle, with grid P2 and unbiased analysis. Additionally, immunohistochemistry studies were performed to further analyse changes in liver tissue. Several haematological and biochemical parameters of blood of experimental animals were also analysed, as well as local tissue reactions around the implants. Statistical analysis was performed using parametric (anova and t-test) and nonparametric tests (Kruskal-Wallis and Mann-Whitney U-test) depending on data distribution. Results Implanted dental cements led to an increase in stereological and histological parameters in liver tissue compared to control rats. Although the investigated parameters mostly showed significant differences between control and experimental animals, the liver tissue of the experimental animals did not have visible signs of pathological changes. This was supported by the analysis of blood parameters which were not significantly different between control and experimental animals. Also, the subcutaneous tissues had minimal inflammatory reactions. Immunohistochemistry studies revealed that nanostructured materials induced proliferation of hepatocytes, but that the immunological response to the materials was not strong enough to induce proliferation of immunoreactive cells in liver in the observed time period. Conclusions This study was performed as a contribution to the attestation of the biocompatibility of dental cements based on calcium silicate and calcium aluminate. Although these materials induced several changes in the liver structure, they were not clinically relevant and represent a normal and reversible response of the liver to the presence of biocompatible materials in the body. Blood and immunohistochemistry analyses and local tissue reactions further confirmed that these materials possess good biocompatible potential.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis
VL  - 52
IS  - 8
SP  - 1162
EP  - 1172
DO  - 10.1111/iej.13105
ER  - 
@article{
author = "Paras, S. and Janković, Ognjenka and Trišić, Dijana and Čolović, Božana and Mitrović-Ajtić, O. and Dekić, R. and Soldatović, Ivan and Živković-Sandić, Marija and Živković, Slavoljub and Jokanović, Vukoman",
year = "2019",
abstract = "Aim To examine the potential systemic toxicity of nanostructured materials based on calcium silicate and calcium aluminate, for potential application in Dentistry. Methodology Twenty-four Albino Wistar rats aged 2 months were used as an in vivo animal model for subcutaneous implantation of the investigated materials, placed in polyethylene tubes. Thirty days after implantation, the livers of the rats were analysed and following histological and stereological parameters were evaluated for volume density of hepatocytes and blood sinusoids, number and numerical density of hepatocytes, surface of hepatocytes and their nucleuses, nucleocytoplasmic ratio and mitotic index of hepatocytes. Stereological measurements were achieved using Cavalieri's principle, with grid P2 and unbiased analysis. Additionally, immunohistochemistry studies were performed to further analyse changes in liver tissue. Several haematological and biochemical parameters of blood of experimental animals were also analysed, as well as local tissue reactions around the implants. Statistical analysis was performed using parametric (anova and t-test) and nonparametric tests (Kruskal-Wallis and Mann-Whitney U-test) depending on data distribution. Results Implanted dental cements led to an increase in stereological and histological parameters in liver tissue compared to control rats. Although the investigated parameters mostly showed significant differences between control and experimental animals, the liver tissue of the experimental animals did not have visible signs of pathological changes. This was supported by the analysis of blood parameters which were not significantly different between control and experimental animals. Also, the subcutaneous tissues had minimal inflammatory reactions. Immunohistochemistry studies revealed that nanostructured materials induced proliferation of hepatocytes, but that the immunological response to the materials was not strong enough to induce proliferation of immunoreactive cells in liver in the observed time period. Conclusions This study was performed as a contribution to the attestation of the biocompatibility of dental cements based on calcium silicate and calcium aluminate. Although these materials induced several changes in the liver structure, they were not clinically relevant and represent a normal and reversible response of the liver to the presence of biocompatible materials in the body. Blood and immunohistochemistry analyses and local tissue reactions further confirmed that these materials possess good biocompatible potential.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis",
volume = "52",
number = "8",
pages = "1162-1172",
doi = "10.1111/iej.13105"
}
Paras, S., Janković, O., Trišić, D., Čolović, B., Mitrović-Ajtić, O., Dekić, R., Soldatović, I., Živković-Sandić, M., Živković, S.,& Jokanović, V.. (2019). Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis. in International Endodontic Journal
Wiley, Hoboken., 52(8), 1162-1172.
https://doi.org/10.1111/iej.13105
Paras S, Janković O, Trišić D, Čolović B, Mitrović-Ajtić O, Dekić R, Soldatović I, Živković-Sandić M, Živković S, Jokanović V. Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis. in International Endodontic Journal. 2019;52(8):1162-1172.
doi:10.1111/iej.13105 .
Paras, S., Janković, Ognjenka, Trišić, Dijana, Čolović, Božana, Mitrović-Ajtić, O., Dekić, R., Soldatović, Ivan, Živković-Sandić, Marija, Živković, Slavoljub, Jokanović, Vukoman, "Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis" in International Endodontic Journal, 52, no. 8 (2019):1162-1172,
https://doi.org/10.1111/iej.13105 . .
9
6
9

Influence of different physical and chemical modifications of titanium surface on its citocompatibility and immunomodulatory properties

Magić, Marko

(Univerzitet u Beogradu, Stomatološki fakultet, 2018)

TY  - THES
AU  - Magić, Marko
PY  - 2018
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6593
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:19386/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50897679
UR  - http://nardus.mpn.gov.rs/123456789/10746
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1040
AB  - Introduction: Titanium and its alloys are being used as dental implant materials due to its biocompatibility and solid physical and mechanical properties. Corrosion properties of titanium and its alloys in acid and alkaline solutions and biological fluids are not satisfying and can be surpassed by modifying its surface area. Biofunctionality of the modified surface depends on the techniques being used. There are many methods to modify titanium surface such as chemical methods that, together with subsequent thermal treatment, provide the formation of a titanium oxide layer, or physical methods, such as deposition of a hydroxyapatite coatings (HAP). Dental implants can increase production of cytokines and chemokines with proinflammatory properties. Aim: Investigation of the effect of the titanium alloy (Ti6Al4V) surface modification by chemical treatment and subsequent thermal treatment, and deposition of a hydroxyapatite coating, on biocompatibility, assesed by cytotoxicity evaluation in vitro, and on the immunomodulatory action of titanium in vitro. Material and methods: Cytotoxicity was determined by using in vitro test of direct contact of titanium alloy samples with L929 cells or indirectly, by determining metabolic activity of L929 cells in the presence of different solutions of conditioned medium (KM). Mouse fibroblast (L929) cell line was used. Metabolic activity was determined with MTT test, cell proliferation was measured with 3[H+]-thymidine test, while reactive oxygen species (ROS) production was determined by flow cytometry after labeling with dichloro-dihydro-fluorescein diacetate (DCFH-DA). Necrosis and apoptosis were measured with propidium iodide (PI) cell staining, measuring lactate dehydrogenase (LDH) production, and by analyzing gene expression of mice caspases 3, 8 and 9 with Real Time PCR method. Cell growth and morphological analysis of L929 cells were determined with phase contrast light microscopy, while viability was determined with Trypan Blue. Immunomodulating effect of the modified titanium surfaces was measured on phytohemaglutinin (PHA) model of peripheral blood mononuclear cells (PBMNC)...
AB  - Titan i njegove legure se upotrebljavaju kao materijali za dentalne implantate zbog pokazane biokompatibilnosti titana i dobrih fizičkih i mehaničkih svojstva. Korozivna svojstva titana i njegovih legura u kiselim i baznim rastvorima, i u biološkim fluidima nisu zadovoljavajuća što je moguće prevazići modifikacijom njihove površine. Biofunkcionalnost modifikovanih površina titana zavisi od tehnika kojima se sprovode modifikacije. Postoje brojne metode modifikacije površine titana, kao hemijske metode koje zajedno sa naknadnim termičkim tretmanom obezbeđuju formiranje titan-oksidnog sloja ili fizičke metode nanošenja prevlaka hidroksiapatita (HAP). Dentalni implantati mogu dovesti do povećane produkcije citokina i hemokina sa proinflamacijskim delovanjem. Cilj istraživanja: Ispitivanje efekta modifikacije površine titanove legure (Ti6Al4V) hemijskom obradom i naknadnim termalnim tretmanom, i nanošenjem prevlake hidroksiapatita, na biokompatibilnost merenu pomoću određivanja citotoksičnosti in vitro, kao i na imunomodulacijsko delovanje titana in vitro. Materijal i metode: Citotoksičnost je ispitivana in vitro, primenom testova direktnog kontakta uzoraka titanovih legura sa L929 ćelijama ili indirektno, ispitivanjem metaboličke aktivnosti L929 ćelija u prisustvu različitih razblaženja kondicioniranog medijuma (KM). Korišćena je ćelijska linija mišijih fibroblasta (L929). Metabolička aktivnost je merena MTT testom, ćelijska proliferacija je merena testom ugradnje 3[H+]-timidina, dok je produkcija slobodnih radikala kiseonika (ROS) određivana protočnom citofluorimetrijom nakon obeležavanja sa dihloro-dihidro-fluorescin diacetatom (DCFH-DA). Nekroza i apoptoza su merene bojenjem ćelija sa propidijum jodidom, merenjem produkcije laktatdehidrogenaze (LDH), kao i analizom ekspresije gena za mišje kaspaze 3, 8 i 9 metodom lančane reakcije polimeraze u realnom vremenu (Real-Time PCR). Ćelijski rast i morfološka analiza L929 ćelija je procenjivana fazno kontrasno svetlosnom mikroskopijom, dok je vijabilnost određivana pomoću tripan plavog. Imunomodulacijski efekat modifikovanih površina titana je ispitivan na modelu fitohemaglutininom (PHA) aktiviranih humanih mononuklearnih ćelija periferne krvi (PBMNC)...
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - Influence of different physical and chemical modifications of titanium surface on its citocompatibility and immunomodulatory properties
T1  - Uticaj različitih modifikacija površine titana fizičkim i hemijskim metodama na njegovu citokompatibilnost i imunomodulacijska svojstva
UR  - https://hdl.handle.net/21.15107/rcub_nardus_10746
ER  - 
@phdthesis{
author = "Magić, Marko",
year = "2018",
abstract = "Introduction: Titanium and its alloys are being used as dental implant materials due to its biocompatibility and solid physical and mechanical properties. Corrosion properties of titanium and its alloys in acid and alkaline solutions and biological fluids are not satisfying and can be surpassed by modifying its surface area. Biofunctionality of the modified surface depends on the techniques being used. There are many methods to modify titanium surface such as chemical methods that, together with subsequent thermal treatment, provide the formation of a titanium oxide layer, or physical methods, such as deposition of a hydroxyapatite coatings (HAP). Dental implants can increase production of cytokines and chemokines with proinflammatory properties. Aim: Investigation of the effect of the titanium alloy (Ti6Al4V) surface modification by chemical treatment and subsequent thermal treatment, and deposition of a hydroxyapatite coating, on biocompatibility, assesed by cytotoxicity evaluation in vitro, and on the immunomodulatory action of titanium in vitro. Material and methods: Cytotoxicity was determined by using in vitro test of direct contact of titanium alloy samples with L929 cells or indirectly, by determining metabolic activity of L929 cells in the presence of different solutions of conditioned medium (KM). Mouse fibroblast (L929) cell line was used. Metabolic activity was determined with MTT test, cell proliferation was measured with 3[H+]-thymidine test, while reactive oxygen species (ROS) production was determined by flow cytometry after labeling with dichloro-dihydro-fluorescein diacetate (DCFH-DA). Necrosis and apoptosis were measured with propidium iodide (PI) cell staining, measuring lactate dehydrogenase (LDH) production, and by analyzing gene expression of mice caspases 3, 8 and 9 with Real Time PCR method. Cell growth and morphological analysis of L929 cells were determined with phase contrast light microscopy, while viability was determined with Trypan Blue. Immunomodulating effect of the modified titanium surfaces was measured on phytohemaglutinin (PHA) model of peripheral blood mononuclear cells (PBMNC)..., Titan i njegove legure se upotrebljavaju kao materijali za dentalne implantate zbog pokazane biokompatibilnosti titana i dobrih fizičkih i mehaničkih svojstva. Korozivna svojstva titana i njegovih legura u kiselim i baznim rastvorima, i u biološkim fluidima nisu zadovoljavajuća što je moguće prevazići modifikacijom njihove površine. Biofunkcionalnost modifikovanih površina titana zavisi od tehnika kojima se sprovode modifikacije. Postoje brojne metode modifikacije površine titana, kao hemijske metode koje zajedno sa naknadnim termičkim tretmanom obezbeđuju formiranje titan-oksidnog sloja ili fizičke metode nanošenja prevlaka hidroksiapatita (HAP). Dentalni implantati mogu dovesti do povećane produkcije citokina i hemokina sa proinflamacijskim delovanjem. Cilj istraživanja: Ispitivanje efekta modifikacije površine titanove legure (Ti6Al4V) hemijskom obradom i naknadnim termalnim tretmanom, i nanošenjem prevlake hidroksiapatita, na biokompatibilnost merenu pomoću određivanja citotoksičnosti in vitro, kao i na imunomodulacijsko delovanje titana in vitro. Materijal i metode: Citotoksičnost je ispitivana in vitro, primenom testova direktnog kontakta uzoraka titanovih legura sa L929 ćelijama ili indirektno, ispitivanjem metaboličke aktivnosti L929 ćelija u prisustvu različitih razblaženja kondicioniranog medijuma (KM). Korišćena je ćelijska linija mišijih fibroblasta (L929). Metabolička aktivnost je merena MTT testom, ćelijska proliferacija je merena testom ugradnje 3[H+]-timidina, dok je produkcija slobodnih radikala kiseonika (ROS) određivana protočnom citofluorimetrijom nakon obeležavanja sa dihloro-dihidro-fluorescin diacetatom (DCFH-DA). Nekroza i apoptoza su merene bojenjem ćelija sa propidijum jodidom, merenjem produkcije laktatdehidrogenaze (LDH), kao i analizom ekspresije gena za mišje kaspaze 3, 8 i 9 metodom lančane reakcije polimeraze u realnom vremenu (Real-Time PCR). Ćelijski rast i morfološka analiza L929 ćelija je procenjivana fazno kontrasno svetlosnom mikroskopijom, dok je vijabilnost određivana pomoću tripan plavog. Imunomodulacijski efekat modifikovanih površina titana je ispitivan na modelu fitohemaglutininom (PHA) aktiviranih humanih mononuklearnih ćelija periferne krvi (PBMNC)...",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "Influence of different physical and chemical modifications of titanium surface on its citocompatibility and immunomodulatory properties, Uticaj različitih modifikacija površine titana fizičkim i hemijskim metodama na njegovu citokompatibilnost i imunomodulacijska svojstva",
url = "https://hdl.handle.net/21.15107/rcub_nardus_10746"
}
Magić, M.. (2018). Influence of different physical and chemical modifications of titanium surface on its citocompatibility and immunomodulatory properties. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_10746
Magić M. Influence of different physical and chemical modifications of titanium surface on its citocompatibility and immunomodulatory properties. 2018;.
https://hdl.handle.net/21.15107/rcub_nardus_10746 .
Magić, Marko, "Influence of different physical and chemical modifications of titanium surface on its citocompatibility and immunomodulatory properties" (2018),
https://hdl.handle.net/21.15107/rcub_nardus_10746 .

Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates

Jokanović, Vukoman; Čolović, Božana; Prokić, Bogomir Bolka; Tomanović, Nada; Popović-Bajić, Marijana; Živković, Slavoljub

(Hindawi Ltd, London, 2018)

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Prokić, Bogomir Bolka
AU  - Tomanović, Nada
AU  - Popović-Bajić, Marijana
AU  - Živković, Slavoljub
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2313
AB  - As an alternative to MTA, a new endodontic material based on hydroxyapatite and calcium silicates (ALBO-MPSA) has been synthesized, and its biocompatibility has been studied in many in vitro and in vivo studies. The current study aims to evaluate a subchronic systemic toxicity of ALBO-MPSA on the rat animal model, as a continuation of the previous studies. Biochemical parameters of blood and histological parameters of the liver, kidneys, and spleen of the rats were analyzed after 120 days of consumption of the aqueous extract of ALBO-MPSA. The results showed no myelotoxic effect or autoimmune effect on peripheral blood cells and no pathological effect on the liver, kidney, and spleen tissues. Besides, no changes in the skin and hair of the rats, neither the change in the consumption of food and water, nor the change in their usual behavior were noticed during the experiment.
PB  - Hindawi Ltd, London
T2  - Advances in Materials Science & Engineering
T1  - Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates
VL  - 2018
DO  - 10.1155/2018/8493439
ER  - 
@article{
author = "Jokanović, Vukoman and Čolović, Božana and Prokić, Bogomir Bolka and Tomanović, Nada and Popović-Bajić, Marijana and Živković, Slavoljub",
year = "2018",
abstract = "As an alternative to MTA, a new endodontic material based on hydroxyapatite and calcium silicates (ALBO-MPSA) has been synthesized, and its biocompatibility has been studied in many in vitro and in vivo studies. The current study aims to evaluate a subchronic systemic toxicity of ALBO-MPSA on the rat animal model, as a continuation of the previous studies. Biochemical parameters of blood and histological parameters of the liver, kidneys, and spleen of the rats were analyzed after 120 days of consumption of the aqueous extract of ALBO-MPSA. The results showed no myelotoxic effect or autoimmune effect on peripheral blood cells and no pathological effect on the liver, kidney, and spleen tissues. Besides, no changes in the skin and hair of the rats, neither the change in the consumption of food and water, nor the change in their usual behavior were noticed during the experiment.",
publisher = "Hindawi Ltd, London",
journal = "Advances in Materials Science & Engineering",
title = "Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates",
volume = "2018",
doi = "10.1155/2018/8493439"
}
Jokanović, V., Čolović, B., Prokić, B. B., Tomanović, N., Popović-Bajić, M.,& Živković, S.. (2018). Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates. in Advances in Materials Science & Engineering
Hindawi Ltd, London., 2018.
https://doi.org/10.1155/2018/8493439
Jokanović V, Čolović B, Prokić BB, Tomanović N, Popović-Bajić M, Živković S. Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates. in Advances in Materials Science & Engineering. 2018;2018.
doi:10.1155/2018/8493439 .
Jokanović, Vukoman, Čolović, Božana, Prokić, Bogomir Bolka, Tomanović, Nada, Popović-Bajić, Marijana, Živković, Slavoljub, "Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates" in Advances in Materials Science & Engineering, 2018 (2018),
https://doi.org/10.1155/2018/8493439 . .
3
2
2

Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study

Ćetenović, Bojana; Čolović, Božana; Vasilijić, Saša; Prokić, Bogomir; Pašalić, Snežana; Jokanović, Vukoman; Tepavčević, Zvezdana; Marković, Dejan

(Springer, Dordrecht, 2018)

TY  - JOUR
AU  - Ćetenović, Bojana
AU  - Čolović, Božana
AU  - Vasilijić, Saša
AU  - Prokić, Bogomir
AU  - Pašalić, Snežana
AU  - Jokanović, Vukoman
AU  - Tepavčević, Zvezdana
AU  - Marković, Dejan
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2275
AB  - The aim of this study was to investigate the biocompatibility of nanostructured materials based on highly active calcium silicates mixed with different radiocontrast agents in comparison to MTA(+) using in vitro and in vivo model. Morphology of materials' samples was analyzed using SEM while the phase compositions were identified by XRD. pH values of materials' suspensions were conducted by pH-meter. The cytotoxicity of materials' solutions was tested by MTT test (100, 50, 25 and 12.5mg/ml). LDH and H-3-thymidine assay were utilized for biocompatibility investigations of materials' eluates (24h, 7 day and 21 day). Eighteen Guinea pigs were used for intramuscular implantation, as teflon tubes with freshly prepared materials were placed into intramuscular pockets. All samples were composed of round and needle-like particles equally distributed with Ca/Si ratio 2.7 at%, with the presence of hydrated calcium silicate phases. The pH values of ALBO-MPCA(1) and ALBO-MPCA(2) were high alkaline, while in case of MTA(+) they were lower and continuously declined (p lt 0.05). Investigated materials didn't exhibit dose-dependent effect on metabolic activity of L929 cells (p>0.05). Significant differences in the percentage of cytotoxicity between diluted and undiluted extracts between all tested materials after 24h and 7 day were noticed (p lt 0.05). Increase in L929 cells proliferation was noticed in case of undiluted eluates of ALBO-MPCA(1) and ALBO-MPCA(2) after 7 day (p lt 0.05). There were no statistically significant differences in the intensity of inflammatory response between investigated materials and control group after 60 day (p>0.05). Evaluation of biocompatibility of both ALBO-MPCA(1) and ALBO-MPCA(2) indicate their potential clinical use. [GRAPHICS] .
PB  - Springer, Dordrecht
T2  - Journal of Materials Science-Materials in Medicine
T1  - Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study
VL  - 29
IS  - 12
DO  - 10.1007/s10856-018-6200-z
ER  - 
@article{
author = "Ćetenović, Bojana and Čolović, Božana and Vasilijić, Saša and Prokić, Bogomir and Pašalić, Snežana and Jokanović, Vukoman and Tepavčević, Zvezdana and Marković, Dejan",
year = "2018",
abstract = "The aim of this study was to investigate the biocompatibility of nanostructured materials based on highly active calcium silicates mixed with different radiocontrast agents in comparison to MTA(+) using in vitro and in vivo model. Morphology of materials' samples was analyzed using SEM while the phase compositions were identified by XRD. pH values of materials' suspensions were conducted by pH-meter. The cytotoxicity of materials' solutions was tested by MTT test (100, 50, 25 and 12.5mg/ml). LDH and H-3-thymidine assay were utilized for biocompatibility investigations of materials' eluates (24h, 7 day and 21 day). Eighteen Guinea pigs were used for intramuscular implantation, as teflon tubes with freshly prepared materials were placed into intramuscular pockets. All samples were composed of round and needle-like particles equally distributed with Ca/Si ratio 2.7 at%, with the presence of hydrated calcium silicate phases. The pH values of ALBO-MPCA(1) and ALBO-MPCA(2) were high alkaline, while in case of MTA(+) they were lower and continuously declined (p lt 0.05). Investigated materials didn't exhibit dose-dependent effect on metabolic activity of L929 cells (p>0.05). Significant differences in the percentage of cytotoxicity between diluted and undiluted extracts between all tested materials after 24h and 7 day were noticed (p lt 0.05). Increase in L929 cells proliferation was noticed in case of undiluted eluates of ALBO-MPCA(1) and ALBO-MPCA(2) after 7 day (p lt 0.05). There were no statistically significant differences in the intensity of inflammatory response between investigated materials and control group after 60 day (p>0.05). Evaluation of biocompatibility of both ALBO-MPCA(1) and ALBO-MPCA(2) indicate their potential clinical use. [GRAPHICS] .",
publisher = "Springer, Dordrecht",
journal = "Journal of Materials Science-Materials in Medicine",
title = "Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study",
volume = "29",
number = "12",
doi = "10.1007/s10856-018-6200-z"
}
Ćetenović, B., Čolović, B., Vasilijić, S., Prokić, B., Pašalić, S., Jokanović, V., Tepavčević, Z.,& Marković, D.. (2018). Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study. in Journal of Materials Science-Materials in Medicine
Springer, Dordrecht., 29(12).
https://doi.org/10.1007/s10856-018-6200-z
Ćetenović B, Čolović B, Vasilijić S, Prokić B, Pašalić S, Jokanović V, Tepavčević Z, Marković D. Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study. in Journal of Materials Science-Materials in Medicine. 2018;29(12).
doi:10.1007/s10856-018-6200-z .
Ćetenović, Bojana, Čolović, Božana, Vasilijić, Saša, Prokić, Bogomir, Pašalić, Snežana, Jokanović, Vukoman, Tepavčević, Zvezdana, Marković, Dejan, "Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study" in Journal of Materials Science-Materials in Medicine, 29, no. 12 (2018),
https://doi.org/10.1007/s10856-018-6200-z . .
4
4
6

Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers

Ćetenović, Bojana; Prokić, Bogomir; Vasilijić, Saša; Dojčinović, Biljana; Magić, Marko; Jokanović, Vukoman; Marković, Dejan

(Elsevier Science Inc, New York, 2017)

TY  - JOUR
AU  - Ćetenović, Bojana
AU  - Prokić, Bogomir
AU  - Vasilijić, Saša
AU  - Dojčinović, Biljana
AU  - Magić, Marko
AU  - Jokanović, Vukoman
AU  - Marković, Dejan
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2196
AB  - Introduction: The aim of this article was to analyze biocompatibility and bioactivity of new endodontic materials on the basis of nanosynthesized calcium silicates (ALBO-MPCA(1) and ALBO-MPCA(2)) combined with different radiopacifiers in comparison with MTA(+). Methods: Morphology of the samples was studied by scanning electron microscopy, and the pH and ion release analysis were also assessed. Biocompatibility of materials' eluates (24-hour, 7-day, and 21-day) was conducted by using MIT test. Twelve New Zealand white rabbits were used for intraosseous implantation. Four calvarial defects per animal were created and filled with freshly prepared investigated materials. Results: Samples mostly consisted of agglomerates built up from nanoparticles, preferably spherical and rod-like. There was no significant difference among pH values of materials' eluates after 24 hours (P>.05). The amount of calcium and aluminum ion release decreased, whereas the amount of magnesium and bismuth (ALBO-MPCAl, MTA(+)) and barium (ALBO-MPCA(2)) increased during 21-day period. The metabolic activity of cells increased after the extraction time, except in case of undiluted elutes of ALBO-MPCA(2) and ALBO-MPCAI (21-day). Histologic analysis of the samples revealed newly formed bone tissue with moderate inflammation for all investigated materials, which subsided during 90-day period to mild. Both MIA(+) and ALBO-MPCAI were in direct contact with the newly formed bone tissue. After 90 days, statistically significant difference in hard tissue formation was observed in comparison of MIA(+) and ALBO-MPCki with control group (P  lt .05). Conclusions: Experimental materials ALBOMPCA, and ALBO-MPCA(2) possess both biocompatibility and bioactivity. Because ALBO-MPCAA provokes favorable biological response, it is especially good candidate for further clinical investigations.
PB  - Elsevier Science Inc, New York
T2  - Journal of Endodontics
T1  - Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers
VL  - 43
IS  - 3
SP  - 425
EP  - 432
DO  - 10.1016/j.joen.2016.10.041
ER  - 
@article{
author = "Ćetenović, Bojana and Prokić, Bogomir and Vasilijić, Saša and Dojčinović, Biljana and Magić, Marko and Jokanović, Vukoman and Marković, Dejan",
year = "2017",
abstract = "Introduction: The aim of this article was to analyze biocompatibility and bioactivity of new endodontic materials on the basis of nanosynthesized calcium silicates (ALBO-MPCA(1) and ALBO-MPCA(2)) combined with different radiopacifiers in comparison with MTA(+). Methods: Morphology of the samples was studied by scanning electron microscopy, and the pH and ion release analysis were also assessed. Biocompatibility of materials' eluates (24-hour, 7-day, and 21-day) was conducted by using MIT test. Twelve New Zealand white rabbits were used for intraosseous implantation. Four calvarial defects per animal were created and filled with freshly prepared investigated materials. Results: Samples mostly consisted of agglomerates built up from nanoparticles, preferably spherical and rod-like. There was no significant difference among pH values of materials' eluates after 24 hours (P>.05). The amount of calcium and aluminum ion release decreased, whereas the amount of magnesium and bismuth (ALBO-MPCAl, MTA(+)) and barium (ALBO-MPCA(2)) increased during 21-day period. The metabolic activity of cells increased after the extraction time, except in case of undiluted elutes of ALBO-MPCA(2) and ALBO-MPCAI (21-day). Histologic analysis of the samples revealed newly formed bone tissue with moderate inflammation for all investigated materials, which subsided during 90-day period to mild. Both MIA(+) and ALBO-MPCAI were in direct contact with the newly formed bone tissue. After 90 days, statistically significant difference in hard tissue formation was observed in comparison of MIA(+) and ALBO-MPCki with control group (P  lt .05). Conclusions: Experimental materials ALBOMPCA, and ALBO-MPCA(2) possess both biocompatibility and bioactivity. Because ALBO-MPCAA provokes favorable biological response, it is especially good candidate for further clinical investigations.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Endodontics",
title = "Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers",
volume = "43",
number = "3",
pages = "425-432",
doi = "10.1016/j.joen.2016.10.041"
}
Ćetenović, B., Prokić, B., Vasilijić, S., Dojčinović, B., Magić, M., Jokanović, V.,& Marković, D.. (2017). Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers. in Journal of Endodontics
Elsevier Science Inc, New York., 43(3), 425-432.
https://doi.org/10.1016/j.joen.2016.10.041
Ćetenović B, Prokić B, Vasilijić S, Dojčinović B, Magić M, Jokanović V, Marković D. Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers. in Journal of Endodontics. 2017;43(3):425-432.
doi:10.1016/j.joen.2016.10.041 .
Ćetenović, Bojana, Prokić, Bogomir, Vasilijić, Saša, Dojčinović, Biljana, Magić, Marko, Jokanović, Vukoman, Marković, Dejan, "Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers" in Journal of Endodontics, 43, no. 3 (2017):425-432,
https://doi.org/10.1016/j.joen.2016.10.041 . .
3
9
9
10

Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation

Jokanović, Vukoman; Čolović, Božana; Marković, Dejan; Petrović, Milan; Soldatović, Ivan; Antonijević, Đorđe; Milosavljević, Petar; Sjerobabin, Nikola; Sopta, Jelena

(Walter De Gruyter Gmbh, Berlin, 2017)

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Marković, Dejan
AU  - Petrović, Milan
AU  - Soldatović, Ivan
AU  - Antonijević, Đorđe
AU  - Milosavljević, Petar
AU  - Sjerobabin, Nikola
AU  - Sopta, Jelena
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2187
AB  - This study examined the potential of a new porous calcium hydroxyapatite scaffold covered with poly (lactide-co-glycolide) (PLGA) as a bone substitute, identifying its advantages over Geistlich Bio-Oss (R), considered the gold standard, in in vivo biofunctionality investigations. Structural and morphological properties of the new scaffold were analyzed by scanning electron and atomic force microscopy. The biofunctionality assays were performed on New Zealand white rabbits using new scaffold for filling full-thickness defects of critical size. The evaluated parameters were: the presence of macrophages, giant cells, monoocytes, plasma cells, granulocytes, neo-angiogenesis, fibroplasia, and the percentage of mineralization. Parallel biofunctionality assays were performed using Geistlich Bio-Oss (R). The appearance of bone defects 12 weeks after the new scaffold implantation showed the presence of a small number of typical immune response cells. Furthermore, significantly reduced number of capillary buds, low intensity of fibroplasia and high degree of mineralization in a lamellar pattern indicated that the inflammation process has been almost completely overcome and that the new bone formed was in the final phase of remodeling. All biofunctionality assays proved the new scaffold's suitability as a bone substitute for applications in maxillofacial surgery. It showed numerous biological advantages over Geistlich Bio-Oss (R) which was reflected mainly as a lower number of giant cells surrounding implanted material and higher degree of mineralization in new formed bone.
PB  - Walter De Gruyter Gmbh, Berlin
T2  - Biomedical Engineering - Biomedizinische Technik
T1  - Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation
VL  - 62
IS  - 3
SP  - 295
EP  - 306
DO  - 10.1515/bmt-2015-0164
ER  - 
@article{
author = "Jokanović, Vukoman and Čolović, Božana and Marković, Dejan and Petrović, Milan and Soldatović, Ivan and Antonijević, Đorđe and Milosavljević, Petar and Sjerobabin, Nikola and Sopta, Jelena",
year = "2017",
abstract = "This study examined the potential of a new porous calcium hydroxyapatite scaffold covered with poly (lactide-co-glycolide) (PLGA) as a bone substitute, identifying its advantages over Geistlich Bio-Oss (R), considered the gold standard, in in vivo biofunctionality investigations. Structural and morphological properties of the new scaffold were analyzed by scanning electron and atomic force microscopy. The biofunctionality assays were performed on New Zealand white rabbits using new scaffold for filling full-thickness defects of critical size. The evaluated parameters were: the presence of macrophages, giant cells, monoocytes, plasma cells, granulocytes, neo-angiogenesis, fibroplasia, and the percentage of mineralization. Parallel biofunctionality assays were performed using Geistlich Bio-Oss (R). The appearance of bone defects 12 weeks after the new scaffold implantation showed the presence of a small number of typical immune response cells. Furthermore, significantly reduced number of capillary buds, low intensity of fibroplasia and high degree of mineralization in a lamellar pattern indicated that the inflammation process has been almost completely overcome and that the new bone formed was in the final phase of remodeling. All biofunctionality assays proved the new scaffold's suitability as a bone substitute for applications in maxillofacial surgery. It showed numerous biological advantages over Geistlich Bio-Oss (R) which was reflected mainly as a lower number of giant cells surrounding implanted material and higher degree of mineralization in new formed bone.",
publisher = "Walter De Gruyter Gmbh, Berlin",
journal = "Biomedical Engineering - Biomedizinische Technik",
title = "Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation",
volume = "62",
number = "3",
pages = "295-306",
doi = "10.1515/bmt-2015-0164"
}
Jokanović, V., Čolović, B., Marković, D., Petrović, M., Soldatović, I., Antonijević, Đ., Milosavljević, P., Sjerobabin, N.,& Sopta, J.. (2017). Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation. in Biomedical Engineering - Biomedizinische Technik
Walter De Gruyter Gmbh, Berlin., 62(3), 295-306.
https://doi.org/10.1515/bmt-2015-0164
Jokanović V, Čolović B, Marković D, Petrović M, Soldatović I, Antonijević Đ, Milosavljević P, Sjerobabin N, Sopta J. Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation. in Biomedical Engineering - Biomedizinische Technik. 2017;62(3):295-306.
doi:10.1515/bmt-2015-0164 .
Jokanović, Vukoman, Čolović, Božana, Marković, Dejan, Petrović, Milan, Soldatović, Ivan, Antonijević, Đorđe, Milosavljević, Petar, Sjerobabin, Nikola, Sopta, Jelena, "Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation" in Biomedical Engineering - Biomedizinische Technik, 62, no. 3 (2017):295-306,
https://doi.org/10.1515/bmt-2015-0164 . .
1
11
7
9

Scaffold in bone tissue engineering

Jokanović, Vukoman; Čolović, Božana; Popović-Bajić, Marijana; Živković-Sandić, Marija

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2017)

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Popović-Bajić, Marijana
AU  - Živković-Sandić, Marija
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2252
AB  - Treatment of bone tissue injuries and diseases is still a great challenge for surgeons, but also for researchers who work with materials. Today stem cells are commonly used in bone tissue engineering. However, advances in biocompatible materials design, especially biodegradable porous structure (scaffold) is gaining an important role in the treatment of diseased bone tissue. The basic advantage of these carriers is specifically designed scaffold with defined porosity and pore structure that is favourable for cells settlement. Scaffolds are most commonly used as ceramic brackets because they have excellent characteristics in biodegradation and bioactivity. The process of scaffold production is important because the appropriate technology must ensure control of liquids and reproducibility of scaffold production through standardized process. The aim of this study was to present some of different procedures of scaffold production in bone tissue engineering and point out the advantages and disadvantages of these methods.
AB  - Terapija i lečenje brojnih povreda i oboljenja koštanog tkiva je još uvek veliki izazov za hirurge, ali i za one istraživače koji se bave materijalima. Na polju inženjerstva koštanog tkiva danas se najčešće koriste matične ćelije. Međutim, napredak u dizajniranju biokompatibilnih materijala, a posebno biodegradibilnih poroznih struktura (skafolda) sve više dobija vrlo značajnu ulogu u lečenju obolelih koštanih tkiva. Specifično dizajnirani skafoldi sa definisanom poroznošću i strukturom pora koja je povoljna za naseljavanje ćelija osnovna je prednost ovih nosača. Skafoldi se najčešće koriste kao keramički nosači jer imaju izvanredne osobine vezane za biodegradaciju i jako izraženu bioaktivnost. Postupak izrade skafolda je vrlo važan jer se odgovarajućom tehnologijom mora obezbediti kontrola tečnosti i reproduktivnost izrade skafolda kroz standardizaciju procesa. Cilj ovog rada je bio da se predstave različiti metodološki postupci izrade skafolda u inženjerstvu koštanog tkiva i ukaže na određene prednosti i nedostatke tih metoda.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Scaffold in bone tissue engineering
T1  - Skafoldi u inženjerstvu koštanog tkiva
VL  - 64
IS  - 1
SP  - 32
EP  - 40
DO  - 10.1515/sdj-2017-0004
ER  - 
@article{
author = "Jokanović, Vukoman and Čolović, Božana and Popović-Bajić, Marijana and Živković-Sandić, Marija",
year = "2017",
abstract = "Treatment of bone tissue injuries and diseases is still a great challenge for surgeons, but also for researchers who work with materials. Today stem cells are commonly used in bone tissue engineering. However, advances in biocompatible materials design, especially biodegradable porous structure (scaffold) is gaining an important role in the treatment of diseased bone tissue. The basic advantage of these carriers is specifically designed scaffold with defined porosity and pore structure that is favourable for cells settlement. Scaffolds are most commonly used as ceramic brackets because they have excellent characteristics in biodegradation and bioactivity. The process of scaffold production is important because the appropriate technology must ensure control of liquids and reproducibility of scaffold production through standardized process. The aim of this study was to present some of different procedures of scaffold production in bone tissue engineering and point out the advantages and disadvantages of these methods., Terapija i lečenje brojnih povreda i oboljenja koštanog tkiva je još uvek veliki izazov za hirurge, ali i za one istraživače koji se bave materijalima. Na polju inženjerstva koštanog tkiva danas se najčešće koriste matične ćelije. Međutim, napredak u dizajniranju biokompatibilnih materijala, a posebno biodegradibilnih poroznih struktura (skafolda) sve više dobija vrlo značajnu ulogu u lečenju obolelih koštanih tkiva. Specifično dizajnirani skafoldi sa definisanom poroznošću i strukturom pora koja je povoljna za naseljavanje ćelija osnovna je prednost ovih nosača. Skafoldi se najčešće koriste kao keramički nosači jer imaju izvanredne osobine vezane za biodegradaciju i jako izraženu bioaktivnost. Postupak izrade skafolda je vrlo važan jer se odgovarajućom tehnologijom mora obezbediti kontrola tečnosti i reproduktivnost izrade skafolda kroz standardizaciju procesa. Cilj ovog rada je bio da se predstave različiti metodološki postupci izrade skafolda u inženjerstvu koštanog tkiva i ukaže na određene prednosti i nedostatke tih metoda.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Scaffold in bone tissue engineering, Skafoldi u inženjerstvu koštanog tkiva",
volume = "64",
number = "1",
pages = "32-40",
doi = "10.1515/sdj-2017-0004"
}
Jokanović, V., Čolović, B., Popović-Bajić, M.,& Živković-Sandić, M.. (2017). Scaffold in bone tissue engineering. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 64(1), 32-40.
https://doi.org/10.1515/sdj-2017-0004
Jokanović V, Čolović B, Popović-Bajić M, Živković-Sandić M. Scaffold in bone tissue engineering. in Stomatološki glasnik Srbije. 2017;64(1):32-40.
doi:10.1515/sdj-2017-0004 .
Jokanović, Vukoman, Čolović, Božana, Popović-Bajić, Marijana, Živković-Sandić, Marija, "Scaffold in bone tissue engineering" in Stomatološki glasnik Srbije, 64, no. 1 (2017):32-40,
https://doi.org/10.1515/sdj-2017-0004 . .
2

History of dentistry in central Serbia

Jeremić, Marko; Vuković, Ana; Marković, Dejan; Vuković, Rade; Stanojlović, Ninoslav

(Udruženje stomatologa Balkana, 2016)

TY  - JOUR
AU  - Jeremić, Marko
AU  - Vuković, Ana
AU  - Marković, Dejan
AU  - Vuković, Rade
AU  - Stanojlović, Ninoslav
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2175
AB  - History of dentistry in the Central Serbian District of Jagodina has been influenced by traditional medicine for centuries. Development of dentistry in the region of Jagodina was slow, the level of oral and general hygiene was low and the sanitary prevention was absent. Trained physicians started to practice medicine and dentistry in the first half of the nineteenth century and they were educated in abroad universities. However, common people used to address to these physicians only when the traditional medicine were unable to help. Until the end of the World War II, common, mostly rural people, with the urgent dental treatment need were usually referred to the barbers, healers or empirics in the nearby villages rather than the dentists. Medications used for the urgent dental treatment were balsams and solutions made of herbs. After the World War II, the dental technicians who finished special courses started to practice dentistry. In 1947 the Regional Dental Office in Jagodina was opened and in 1955 the first Doctor of Dental Medicine who graduated from the School of Dental Medicine of University of Belgrade was employed. Nowadays, the Department of Dentistry represents is an important and independent part of the Health Care Centre in Jagodina.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Dental Medicine
T1  - History of dentistry in central Serbia
VL  - 20
IS  - 3
SP  - 138
EP  - 142
DO  - 10.1515/bjdm-2016-0022
ER  - 
@article{
author = "Jeremić, Marko and Vuković, Ana and Marković, Dejan and Vuković, Rade and Stanojlović, Ninoslav",
year = "2016",
abstract = "History of dentistry in the Central Serbian District of Jagodina has been influenced by traditional medicine for centuries. Development of dentistry in the region of Jagodina was slow, the level of oral and general hygiene was low and the sanitary prevention was absent. Trained physicians started to practice medicine and dentistry in the first half of the nineteenth century and they were educated in abroad universities. However, common people used to address to these physicians only when the traditional medicine were unable to help. Until the end of the World War II, common, mostly rural people, with the urgent dental treatment need were usually referred to the barbers, healers or empirics in the nearby villages rather than the dentists. Medications used for the urgent dental treatment were balsams and solutions made of herbs. After the World War II, the dental technicians who finished special courses started to practice dentistry. In 1947 the Regional Dental Office in Jagodina was opened and in 1955 the first Doctor of Dental Medicine who graduated from the School of Dental Medicine of University of Belgrade was employed. Nowadays, the Department of Dentistry represents is an important and independent part of the Health Care Centre in Jagodina.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Dental Medicine",
title = "History of dentistry in central Serbia",
volume = "20",
number = "3",
pages = "138-142",
doi = "10.1515/bjdm-2016-0022"
}
Jeremić, M., Vuković, A., Marković, D., Vuković, R.,& Stanojlović, N.. (2016). History of dentistry in central Serbia. in Balkan Journal of Dental Medicine
Udruženje stomatologa Balkana., 20(3), 138-142.
https://doi.org/10.1515/bjdm-2016-0022
Jeremić M, Vuković A, Marković D, Vuković R, Stanojlović N. History of dentistry in central Serbia. in Balkan Journal of Dental Medicine. 2016;20(3):138-142.
doi:10.1515/bjdm-2016-0022 .
Jeremić, Marko, Vuković, Ana, Marković, Dejan, Vuković, Rade, Stanojlović, Ninoslav, "History of dentistry in central Serbia" in Balkan Journal of Dental Medicine, 20, no. 3 (2016):138-142,
https://doi.org/10.1515/bjdm-2016-0022 . .

After-Hours Versus Office-Hours Dental Injuries in Children: Does Timing Influence Outcome?

Vuković, Ana; Vuković, Rade; Marković, Dejan; Soldatović, Ivan; Mandinić, Zoran; Beloica, Miloš; Stojan, George

(Sage Publications Inc, Thousand Oaks, 2016)

TY  - JOUR
AU  - Vuković, Ana
AU  - Vuković, Rade
AU  - Marković, Dejan
AU  - Soldatović, Ivan
AU  - Mandinić, Zoran
AU  - Beloica, Miloš
AU  - Stojan, George
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2133
AB  - Aim. The aim of this study was to analyze the outcomes and factors associated with after-hours dental trauma. Methods. Study sample consisted of 1762 permanent teeth injuries in children, gender and age matched with office-hours injuries. Epidemiological and clinical data were collected from 4 university dental trauma centers. Results. During median follow-up time of 4.3 years, complications have occurred in 14.5% of injured teeth. Age, type, and degree of tissue injury and after-hours time of injury were significantly associated with complications. Unfavorable outcomes were 34% more likely in the after-hours group compared with office-hours. Urgent treatment was significantly delayed in after-hours group with a delay of more than 3 hours in 90.5% versus 38.9% in the office-hours group. Multivariate regression model showed that after-hours time of injury was significant predictor of complications. Conclusion. Delayed urgent treatment was one of the main factors associated with unfavorable outcome of after-hours injuries.
PB  - Sage Publications Inc, Thousand Oaks
T2  - Clinical Pediatrics
T1  - After-Hours Versus Office-Hours Dental Injuries in Children: Does Timing Influence Outcome?
VL  - 55
IS  - 1
SP  - 29
EP  - 35
DO  - 10.1177/0009922815584214
ER  - 
@article{
author = "Vuković, Ana and Vuković, Rade and Marković, Dejan and Soldatović, Ivan and Mandinić, Zoran and Beloica, Miloš and Stojan, George",
year = "2016",
abstract = "Aim. The aim of this study was to analyze the outcomes and factors associated with after-hours dental trauma. Methods. Study sample consisted of 1762 permanent teeth injuries in children, gender and age matched with office-hours injuries. Epidemiological and clinical data were collected from 4 university dental trauma centers. Results. During median follow-up time of 4.3 years, complications have occurred in 14.5% of injured teeth. Age, type, and degree of tissue injury and after-hours time of injury were significantly associated with complications. Unfavorable outcomes were 34% more likely in the after-hours group compared with office-hours. Urgent treatment was significantly delayed in after-hours group with a delay of more than 3 hours in 90.5% versus 38.9% in the office-hours group. Multivariate regression model showed that after-hours time of injury was significant predictor of complications. Conclusion. Delayed urgent treatment was one of the main factors associated with unfavorable outcome of after-hours injuries.",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Clinical Pediatrics",
title = "After-Hours Versus Office-Hours Dental Injuries in Children: Does Timing Influence Outcome?",
volume = "55",
number = "1",
pages = "29-35",
doi = "10.1177/0009922815584214"
}
Vuković, A., Vuković, R., Marković, D., Soldatović, I., Mandinić, Z., Beloica, M.,& Stojan, G.. (2016). After-Hours Versus Office-Hours Dental Injuries in Children: Does Timing Influence Outcome?. in Clinical Pediatrics
Sage Publications Inc, Thousand Oaks., 55(1), 29-35.
https://doi.org/10.1177/0009922815584214
Vuković A, Vuković R, Marković D, Soldatović I, Mandinić Z, Beloica M, Stojan G. After-Hours Versus Office-Hours Dental Injuries in Children: Does Timing Influence Outcome?. in Clinical Pediatrics. 2016;55(1):29-35.
doi:10.1177/0009922815584214 .
Vuković, Ana, Vuković, Rade, Marković, Dejan, Soldatović, Ivan, Mandinić, Zoran, Beloica, Miloš, Stojan, George, "After-Hours Versus Office-Hours Dental Injuries in Children: Does Timing Influence Outcome?" in Clinical Pediatrics, 55, no. 1 (2016):29-35,
https://doi.org/10.1177/0009922815584214 . .
4
3
2

Biological aspects of application of nanomaterials in tissue engineering

Marković, Dejan; Karadžić, Ivana; Jokanović, Vukoman; Vuković, Ana; Vučić, Vesna

(Savez hemijskih inženjera, Beograd, 2016)

TY  - JOUR
AU  - Marković, Dejan
AU  - Karadžić, Ivana
AU  - Jokanović, Vukoman
AU  - Vuković, Ana
AU  - Vučić, Vesna
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2083
AB  - Millions of patients worldwide need surgery to repair or replace tissue that has been damaged through trauma or disease. To solve the problem of lost tissue, a major emphasis of tissue engineering (TE) is on tissue regeneration. Stem cells and highly porous biomaterials used as cell carriers (scaffolds) have an essential role in the production of new tissue by TE. The cellular component is important for the generation and establishment of the extracellular matrix, while a scaffold is necessary to determine the shape of the newly formed tissue and facilitate migration of cells into the desired location, as well as their growth and differentiation. This review describes the types, characteristics and classification of stem cells. Furthermore, it includes functional features of cell carriers - biocompatibility, biodegradability and mechanical properties of biomaterials used in developing state-of-the-an scaffolds for TE applications, as well as suitability for different tissues. Moreover, it explains the importance of nanotechnology and defines the challenges and the purpose of future research in this rapidly advancing field.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly / CICEQ
T1  - Biological aspects of application of nanomaterials in tissue engineering
VL  - 22
IS  - 2
SP  - 145
EP  - 153
DO  - 10.2298/CICEQ141231028M
ER  - 
@article{
author = "Marković, Dejan and Karadžić, Ivana and Jokanović, Vukoman and Vuković, Ana and Vučić, Vesna",
year = "2016",
abstract = "Millions of patients worldwide need surgery to repair or replace tissue that has been damaged through trauma or disease. To solve the problem of lost tissue, a major emphasis of tissue engineering (TE) is on tissue regeneration. Stem cells and highly porous biomaterials used as cell carriers (scaffolds) have an essential role in the production of new tissue by TE. The cellular component is important for the generation and establishment of the extracellular matrix, while a scaffold is necessary to determine the shape of the newly formed tissue and facilitate migration of cells into the desired location, as well as their growth and differentiation. This review describes the types, characteristics and classification of stem cells. Furthermore, it includes functional features of cell carriers - biocompatibility, biodegradability and mechanical properties of biomaterials used in developing state-of-the-an scaffolds for TE applications, as well as suitability for different tissues. Moreover, it explains the importance of nanotechnology and defines the challenges and the purpose of future research in this rapidly advancing field.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly / CICEQ",
title = "Biological aspects of application of nanomaterials in tissue engineering",
volume = "22",
number = "2",
pages = "145-153",
doi = "10.2298/CICEQ141231028M"
}
Marković, D., Karadžić, I., Jokanović, V., Vuković, A.,& Vučić, V.. (2016). Biological aspects of application of nanomaterials in tissue engineering. in Chemical Industry & Chemical Engineering Quarterly / CICEQ
Savez hemijskih inženjera, Beograd., 22(2), 145-153.
https://doi.org/10.2298/CICEQ141231028M
Marković D, Karadžić I, Jokanović V, Vuković A, Vučić V. Biological aspects of application of nanomaterials in tissue engineering. in Chemical Industry & Chemical Engineering Quarterly / CICEQ. 2016;22(2):145-153.
doi:10.2298/CICEQ141231028M .
Marković, Dejan, Karadžić, Ivana, Jokanović, Vukoman, Vuković, Ana, Vučić, Vesna, "Biological aspects of application of nanomaterials in tissue engineering" in Chemical Industry & Chemical Engineering Quarterly / CICEQ, 22, no. 2 (2016):145-153,
https://doi.org/10.2298/CICEQ141231028M . .
4
2
3

Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design

Sjerobabin, Nikola; Čolović, Božana; Petrović, Milan; Marković, Dejan; Živković, Slavoljub; Jokanović, Vukoman

(Srpsko lekarsko društvo, Beograd, 2016)

TY  - JOUR
AU  - Sjerobabin, Nikola
AU  - Čolović, Božana
AU  - Petrović, Milan
AU  - Marković, Dejan
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2162
AB  - Introduction: Biodegradable porous scaffolds are found to be very promising bone substitutes, acting as a temporary physical support to guide new tissue regeneration, until the entire scaffold is totally degraded and replaced by the new tissue. Objective The aim of this study was to investigate cytotoxicity of a synthesized calcium hydroxyapatite-based scaffold, named ALBO-OS, with high porosity and optimal topology. Methods The ALBO-OS scaffold was synthesized by the method of polymer foam template. The analysis of pore geometry and scaffold walls' topography was made by scanning electron microscope (SEM). The biological investigations assumed the examinations of ALBO-OS cytotoxicity to mouse L929 fibroblasts, using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) and lactate dehydrogenase (LDH) tests and inverse phase microscopy. Results The SEM analysis showed high porosity with fair pore distribution and interesting morphology from the biological standpoint. The biological investigations showed that the material is not cytotoxic to L929 cells. Comparison of ALBO-OS with Bio-Oss, as the global gold standard as a bone substitute, showed similar results in MTT test, while LDH test showed significantly higher rate of cell multiplication with ALBO-OS. Conclusion The scaffold design from the aspect of pore size, distribution, and topology seems to be very convenient for cell adhesion and occupation, which makes it a promising material as a bone substitute. The results of biological assays proved that ALBO-OS is not cytotoxic for L929 fibroblasts. In comparison with Bio-Oss, similar or even better results were obtained.
AB  - Uvod: Porozni biodegradabilni nosači su se pokazali kao dobri zamenici kosti jer deluju kao privremena fizička potpora za usmerenu regeneraciju tkiva dok se čitav nosač potpuno ne razgradi i zameni novim tkivom. Cilj rada Cilj ovog rada je bio da se ispita citotoksičnost nosača tkiva na bazi kalcijum-hidroksiapatita, visoke poroznosti i optimalne topologije, nazvanog ALBO-OS. Metode rada Zamenik kosti ALBO-OS je sintetisan metodom matrice napravljene od polimerne pene. Geometrija pora i zidova nosača analizirane su pomoću skenirajuće elektronske mikroskopije (SEM). Biološka istraživanja izvedena su ispitivanjem citotoksičnosti ALBO-OS-a na mišjim fibroblastima L929 pomoću MTT i LDH testova i fazno kontrastne mikroskopije. Rezultati SEM analiza je pokazala veliku i ravnomernu poroznost i zanimljivu morfologiju sa biološkog stanovišta. Biološka istraživanja pokazala su da materijal nije citotoksičan. Poređenjem materijala ALBO-OS i Bio-Oss, koji je, globalno, zlatni standard među zamenicima kosti, dobijeni su slični rezultati na MTT testu, dok su rezulati LDH testa pokazali značajno veći broj ćelijskih deoba u kontaktu sa ALBO-OS-om. Zaključak Dizajn nosača sa stanovišta raspodele veličine pora i topologije je veoma pogodan za adheziju i naseljavanje ćelija, zbog čega ima veliki potencijal kao zamenik kosti. Rezultati bioloških testova su pokazali da ALBO-OS nije citotoksičan za L929 fibroblaste. U poređenju sa materijalom Bio-Oss, dobijeni su slični ili bolji rezultati.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design
T1  - Ispitivanje citotoksičnosti nosača na bazi hidroksiapatita s unapređenim strukturnim dizajnom
VL  - 144
IS  - 5-6
SP  - 280
EP  - 287
DO  - 10.2298/SARH1606280S
ER  - 
@article{
author = "Sjerobabin, Nikola and Čolović, Božana and Petrović, Milan and Marković, Dejan and Živković, Slavoljub and Jokanović, Vukoman",
year = "2016",
abstract = "Introduction: Biodegradable porous scaffolds are found to be very promising bone substitutes, acting as a temporary physical support to guide new tissue regeneration, until the entire scaffold is totally degraded and replaced by the new tissue. Objective The aim of this study was to investigate cytotoxicity of a synthesized calcium hydroxyapatite-based scaffold, named ALBO-OS, with high porosity and optimal topology. Methods The ALBO-OS scaffold was synthesized by the method of polymer foam template. The analysis of pore geometry and scaffold walls' topography was made by scanning electron microscope (SEM). The biological investigations assumed the examinations of ALBO-OS cytotoxicity to mouse L929 fibroblasts, using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) and lactate dehydrogenase (LDH) tests and inverse phase microscopy. Results The SEM analysis showed high porosity with fair pore distribution and interesting morphology from the biological standpoint. The biological investigations showed that the material is not cytotoxic to L929 cells. Comparison of ALBO-OS with Bio-Oss, as the global gold standard as a bone substitute, showed similar results in MTT test, while LDH test showed significantly higher rate of cell multiplication with ALBO-OS. Conclusion The scaffold design from the aspect of pore size, distribution, and topology seems to be very convenient for cell adhesion and occupation, which makes it a promising material as a bone substitute. The results of biological assays proved that ALBO-OS is not cytotoxic for L929 fibroblasts. In comparison with Bio-Oss, similar or even better results were obtained., Uvod: Porozni biodegradabilni nosači su se pokazali kao dobri zamenici kosti jer deluju kao privremena fizička potpora za usmerenu regeneraciju tkiva dok se čitav nosač potpuno ne razgradi i zameni novim tkivom. Cilj rada Cilj ovog rada je bio da se ispita citotoksičnost nosača tkiva na bazi kalcijum-hidroksiapatita, visoke poroznosti i optimalne topologije, nazvanog ALBO-OS. Metode rada Zamenik kosti ALBO-OS je sintetisan metodom matrice napravljene od polimerne pene. Geometrija pora i zidova nosača analizirane su pomoću skenirajuće elektronske mikroskopije (SEM). Biološka istraživanja izvedena su ispitivanjem citotoksičnosti ALBO-OS-a na mišjim fibroblastima L929 pomoću MTT i LDH testova i fazno kontrastne mikroskopije. Rezultati SEM analiza je pokazala veliku i ravnomernu poroznost i zanimljivu morfologiju sa biološkog stanovišta. Biološka istraživanja pokazala su da materijal nije citotoksičan. Poređenjem materijala ALBO-OS i Bio-Oss, koji je, globalno, zlatni standard među zamenicima kosti, dobijeni su slični rezultati na MTT testu, dok su rezulati LDH testa pokazali značajno veći broj ćelijskih deoba u kontaktu sa ALBO-OS-om. Zaključak Dizajn nosača sa stanovišta raspodele veličine pora i topologije je veoma pogodan za adheziju i naseljavanje ćelija, zbog čega ima veliki potencijal kao zamenik kosti. Rezultati bioloških testova su pokazali da ALBO-OS nije citotoksičan za L929 fibroblaste. U poređenju sa materijalom Bio-Oss, dobijeni su slični ili bolji rezultati.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design, Ispitivanje citotoksičnosti nosača na bazi hidroksiapatita s unapređenim strukturnim dizajnom",
volume = "144",
number = "5-6",
pages = "280-287",
doi = "10.2298/SARH1606280S"
}
Sjerobabin, N., Čolović, B., Petrović, M., Marković, D., Živković, S.,& Jokanović, V.. (2016). Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 144(5-6), 280-287.
https://doi.org/10.2298/SARH1606280S
Sjerobabin N, Čolović B, Petrović M, Marković D, Živković S, Jokanović V. Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design. in Srpski arhiv za celokupno lekarstvo. 2016;144(5-6):280-287.
doi:10.2298/SARH1606280S .
Sjerobabin, Nikola, Čolović, Božana, Petrović, Milan, Marković, Dejan, Živković, Slavoljub, Jokanović, Vukoman, "Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design" in Srpski arhiv za celokupno lekarstvo, 144, no. 5-6 (2016):280-287,
https://doi.org/10.2298/SARH1606280S . .
1
1
1

In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA

Jokanović, Vukoman; Čolović, Božana; Marković, Dejan; Petrović, Milan; Jokanović, Milan; Milosavljević, Petar; Sopta, Jelena

(Hindawi Ltd, London, 2016)

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Marković, Dejan
AU  - Petrović, Milan
AU  - Jokanović, Milan
AU  - Milosavljević, Petar
AU  - Sopta, Jelena
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2168
AB  - A synthetic bone substitute based on calcium hydroxyapatite (CHA) and poly(lactic-co-glycolic) acid (PLGA), described in this paper, was synthesized to fulfill specific requirements like biodegradability, satisfying mechanical properties, optimal porosity and nanotopology, osteoinductive and osteoconductive properties, and so forth. Structural and morphological properties of the new scaffold were analyzed by micro computed tomography and scanning electron microscopy, while its physicochemical properties were investigated by X-ray diffraction and infrared spectroscopy. In vivo biological investigations of the synthesized scaffold were conducted over the cutaneous irritation and biofunctionality assays on rabbits and the test of acute systemic toxicity on mice. The results showed that the scaffold is not irritant and that it does not exhibit any symptoms of acute toxicity. Biofunctionality assays which include evaluation of the presence of various cells of immune response, the presence of neoangiogenesis, percentage of mineralization of newly formed bone, and fibroplasia in the tissue indicated that the new scaffold is suitable for the application in maxillofacial and dental surgery as a bone substitute. Also, it showed significant advantages over commercial product Geistlich Bio-Oss(R) from the aspect of some parameters of immunological response.
PB  - Hindawi Ltd, London
T2  - Journal of Nanomaterials
T1  - In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA
VL  - 2016
DO  - 10.1155/2016/3948768
ER  - 
@article{
author = "Jokanović, Vukoman and Čolović, Božana and Marković, Dejan and Petrović, Milan and Jokanović, Milan and Milosavljević, Petar and Sopta, Jelena",
year = "2016",
abstract = "A synthetic bone substitute based on calcium hydroxyapatite (CHA) and poly(lactic-co-glycolic) acid (PLGA), described in this paper, was synthesized to fulfill specific requirements like biodegradability, satisfying mechanical properties, optimal porosity and nanotopology, osteoinductive and osteoconductive properties, and so forth. Structural and morphological properties of the new scaffold were analyzed by micro computed tomography and scanning electron microscopy, while its physicochemical properties were investigated by X-ray diffraction and infrared spectroscopy. In vivo biological investigations of the synthesized scaffold were conducted over the cutaneous irritation and biofunctionality assays on rabbits and the test of acute systemic toxicity on mice. The results showed that the scaffold is not irritant and that it does not exhibit any symptoms of acute toxicity. Biofunctionality assays which include evaluation of the presence of various cells of immune response, the presence of neoangiogenesis, percentage of mineralization of newly formed bone, and fibroplasia in the tissue indicated that the new scaffold is suitable for the application in maxillofacial and dental surgery as a bone substitute. Also, it showed significant advantages over commercial product Geistlich Bio-Oss(R) from the aspect of some parameters of immunological response.",
publisher = "Hindawi Ltd, London",
journal = "Journal of Nanomaterials",
title = "In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA",
volume = "2016",
doi = "10.1155/2016/3948768"
}
Jokanović, V., Čolović, B., Marković, D., Petrović, M., Jokanović, M., Milosavljević, P.,& Sopta, J.. (2016). In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA. in Journal of Nanomaterials
Hindawi Ltd, London., 2016.
https://doi.org/10.1155/2016/3948768
Jokanović V, Čolović B, Marković D, Petrović M, Jokanović M, Milosavljević P, Sopta J. In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA. in Journal of Nanomaterials. 2016;2016.
doi:10.1155/2016/3948768 .
Jokanović, Vukoman, Čolović, Božana, Marković, Dejan, Petrović, Milan, Jokanović, Milan, Milosavljević, Petar, Sopta, Jelena, "In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA" in Journal of Nanomaterials, 2016 (2016),
https://doi.org/10.1155/2016/3948768 . .
8
4
5

Structural characteristics and mechanisms of fluorapatite mechanochemical synthesis

Jokanović, Vukoman; Čolović, Božana; Živković-Sandić, Marija; Popović-Bajić, Marijana; Živković, Slavoljub

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2016)

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Živković-Sandić, Marija
AU  - Popović-Bajić, Marijana
AU  - Živković, Slavoljub
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2108
AB  - This paper analyzes mechanisms of fluorapatite mechanochemical synthesis and its structural characteristics. Several studies of Jokanovic et al. published in appropriate journals and the book 'Nanomedicine, the biggest challenge of the 21st century' are the base for this article. Characteristics of obtained materials show numerous biological advantages associated with the specific structural design of material during the process of synthesis. X-ray diffraction (XRD) and infrared spectroscopy with Fourier transform (FTIR) were used for studying the processes of fluorapatite synthesis.
AB  - U radu su analizirani mehanizmi mehanohemijske sinteze fluorapatita i njegove strukturne karakteristike. Suštinu istraživanja čine istraživanja V. Jokanovića i saradnika objavljena u odgovarajućim časopisima i knjizi Nanomedicina, najveći izazov 21. veka. Karakteristike dobijenih materijala pokazuju njihove brojne biološke prednosti, koje su povezane sa specifičnostima strukturnog dizajna materijala tokom procesa sinteze. Kao osnove za proučavanje procesa sinteze i mehanizma nastajanja fluorapatita korišćene su metode rendgenske difrakcije (XRD) i infracrvene spektroskopije sa Furijeovom transformacijom (FTIR).
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Structural characteristics and mechanisms of fluorapatite mechanochemical synthesis
T1  - Strukturne karakteristike i mehanizmi mehanohemijske sinteze fluorapatita
VL  - 63
IS  - 2
SP  - 74
EP  - 84
DO  - 10.1515/sdj-2016-0008
ER  - 
@article{
author = "Jokanović, Vukoman and Čolović, Božana and Živković-Sandić, Marija and Popović-Bajić, Marijana and Živković, Slavoljub",
year = "2016",
abstract = "This paper analyzes mechanisms of fluorapatite mechanochemical synthesis and its structural characteristics. Several studies of Jokanovic et al. published in appropriate journals and the book 'Nanomedicine, the biggest challenge of the 21st century' are the base for this article. Characteristics of obtained materials show numerous biological advantages associated with the specific structural design of material during the process of synthesis. X-ray diffraction (XRD) and infrared spectroscopy with Fourier transform (FTIR) were used for studying the processes of fluorapatite synthesis., U radu su analizirani mehanizmi mehanohemijske sinteze fluorapatita i njegove strukturne karakteristike. Suštinu istraživanja čine istraživanja V. Jokanovića i saradnika objavljena u odgovarajućim časopisima i knjizi Nanomedicina, najveći izazov 21. veka. Karakteristike dobijenih materijala pokazuju njihove brojne biološke prednosti, koje su povezane sa specifičnostima strukturnog dizajna materijala tokom procesa sinteze. Kao osnove za proučavanje procesa sinteze i mehanizma nastajanja fluorapatita korišćene su metode rendgenske difrakcije (XRD) i infracrvene spektroskopije sa Furijeovom transformacijom (FTIR).",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Structural characteristics and mechanisms of fluorapatite mechanochemical synthesis, Strukturne karakteristike i mehanizmi mehanohemijske sinteze fluorapatita",
volume = "63",
number = "2",
pages = "74-84",
doi = "10.1515/sdj-2016-0008"
}
Jokanović, V., Čolović, B., Živković-Sandić, M., Popović-Bajić, M.,& Živković, S.. (2016). Structural characteristics and mechanisms of fluorapatite mechanochemical synthesis. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 63(2), 74-84.
https://doi.org/10.1515/sdj-2016-0008
Jokanović V, Čolović B, Živković-Sandić M, Popović-Bajić M, Živković S. Structural characteristics and mechanisms of fluorapatite mechanochemical synthesis. in Stomatološki glasnik Srbije. 2016;63(2):74-84.
doi:10.1515/sdj-2016-0008 .
Jokanović, Vukoman, Čolović, Božana, Živković-Sandić, Marija, Popović-Bajić, Marijana, Živković, Slavoljub, "Structural characteristics and mechanisms of fluorapatite mechanochemical synthesis" in Stomatološki glasnik Srbije, 63, no. 2 (2016):74-84,
https://doi.org/10.1515/sdj-2016-0008 . .
4

The influence of RUNX2 and WNT10A gene mutations on tooth number and size

Živković-Sandić, Marija

(Univerzitet u Beogradu, Stomatološki fakultet, 2016)

TY  - THES
AU  - Živković-Sandić, Marija
PY  - 2016
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=4680
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:14872/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48770831
UR  - http://nardus.mpn.gov.rs/123456789/7723
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1003
AB  - Hypodontia (tooth agenesis) is one of the most common dental anomalies in humans that occurs either in non-syndromic forms or as a part of various syndroms. Hypodontia can be a result of nucleotide substitutions in genes that are involved in tooth morphogenesis, whose products are signal molecules and transcription factors that control gene expression in different phases of tooth morphogenesis (bud, cap and bell stage). Among many genes, WNT10A (with its expression in dental epithelial tissue) and RUNX2 gene (with its expression in mesenchimal tissue) have been singled out due to their key role in odontogenesis. Mutations of genes involved in odontogenesis can lead to changes in tooth morphology, structure and the number of teeth. The aim of this study was to examine the presence of potential mutations in RUNX2 and WNT10A genes, and to determine their inheritance path in patients with isolated (non-syndromic) forms of tooth agenesis. The second part of the investigation was aimed to compare tooth dimensions in hypodontia patients and healthy controls, in order to determine whether hypodontia / olygodontia is related to potential tooth size anomalies. Mutational status for RUNX2 and WNT10A genes was determined for 24 families. The sample included 72 subjects, 29 of which had clinically confirmed tooth agenesis. Mesiodistal tooth width was measured in 25 hypodontia patients and 25 healthy controls. Genetic mutations presence was confirmed by direct sequencing. Mesiodistal tooth width was measured on digitized study models using a computer software. Mutational analysis of WNT10A gene revealed the presence of two nucleotide substitutions in the exon 2 region (rs10180544, rs141074983) and three nucleotide substitutions in exon 3 (rs121908120, rs77583146, rs146902156). Mutational analysis of RUNX2 gene revealed four nucleotide substitutions in exon 3 (rs6921145, COSM1658842, rs759395776, rs768156797) and one in exon 4 (34 insC). Mutations in WNT10A gene were present in 52% of subjects, mutations in RUNX2 gene in 59% of subjects, mutations in both genes 35% of subjects, and no mutations were found in 24% of the sample...
AB  - Hipodoncija-urodjeni nedostatak zuba, predstavlja jednu od najĉešćih razvojnih anomalija kod ljudi, i moţe biti nesindromska i sindromska. Do razvoja hipodoncije moţe doći usled izmena u genima ukljuĉenim u morfogenezu zuba, a ĉiji produkti su signalni i transkripcioni faktori, odgovorni za vremenski programiranu ekspresiju gena u fazama pupoljka, kape i zvona. Iz palete gena ukljuĉenih u odontogenezu, zbog svog kljuĉnog znaĉaja izdvaja se WNT10A gen (eksprimiran u dentalnom epitelu), i RUNX2 gen (eksprimiran u mezenhimu). Mutacije gena povezanih sa odontogenezom mogu dovesti do izmena u morfologiji, broju i strukturi zuba. Ciljevi ove studije su bili da se ispita zastupljenost potencijalnih mutacija u RUNX2 i WNT10A genima i utvrdi njihov naĉin nasledjivanja kod izolovanih (nesindromskih) formi hipodoncije. Drugi deo istraţivanja odnosio se na uporedjivanje veliĉine zuba pacijenata sa urodjenim nedostatkom zuba i zdravih pacijenata kako bi se utvrdilo da li je hipodoncija/oligodoncija povezana sa eventualnim anomalijama veliĉine zuba . Mutacioni status RUNX2 i WNT10A gena, odreĊen je kod 24 porodice (72 ispitanika), pri ĉemu je kod 29 osoba, kliniĉki potvrdjena hipodoncija. Merenje meziodistalnih širina zuba je uradjeno kod 25 pacijenata sa uroĊenim nedostatkom zuba i 25 pacijenata kontrolne grupe. Prisustvo mutacija potvrĊeno je primenom tehnike direktnog sekvenciranja, dok je odreĊivanje meziodistalnih širina zuba uraĊeno primenom 3D skenera i merenjima na digitalnim modelima u kompjuterskom programu. Mutaciona analiza WNT10A gena, pokazala je prisustvo 2 nukleotidne izmene u regionu egzona 2 (rs10180544, rs141074983) i 3 izmene u egzonu 3 (rs121908120, rs77583146, rs146902156). Mutacionom analizom RUNX2 gena utvrĊeno je prisustvo 4 nukleotidne izmene u egzonu 3 (rs6921145, COSM1658842, rs759395776, rs768156797) i jedna nukleotidna izmena u regionu egzona 4 (34 insC)...
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - The influence of RUNX2 and WNT10A gene mutations on tooth number and size
T1  - Uticaj mutacija RUNX2 i WNT10A gena na broj i veličinu zuba
UR  - https://hdl.handle.net/21.15107/rcub_nardus_7723
ER  - 
@phdthesis{
author = "Živković-Sandić, Marija",
year = "2016",
abstract = "Hypodontia (tooth agenesis) is one of the most common dental anomalies in humans that occurs either in non-syndromic forms or as a part of various syndroms. Hypodontia can be a result of nucleotide substitutions in genes that are involved in tooth morphogenesis, whose products are signal molecules and transcription factors that control gene expression in different phases of tooth morphogenesis (bud, cap and bell stage). Among many genes, WNT10A (with its expression in dental epithelial tissue) and RUNX2 gene (with its expression in mesenchimal tissue) have been singled out due to their key role in odontogenesis. Mutations of genes involved in odontogenesis can lead to changes in tooth morphology, structure and the number of teeth. The aim of this study was to examine the presence of potential mutations in RUNX2 and WNT10A genes, and to determine their inheritance path in patients with isolated (non-syndromic) forms of tooth agenesis. The second part of the investigation was aimed to compare tooth dimensions in hypodontia patients and healthy controls, in order to determine whether hypodontia / olygodontia is related to potential tooth size anomalies. Mutational status for RUNX2 and WNT10A genes was determined for 24 families. The sample included 72 subjects, 29 of which had clinically confirmed tooth agenesis. Mesiodistal tooth width was measured in 25 hypodontia patients and 25 healthy controls. Genetic mutations presence was confirmed by direct sequencing. Mesiodistal tooth width was measured on digitized study models using a computer software. Mutational analysis of WNT10A gene revealed the presence of two nucleotide substitutions in the exon 2 region (rs10180544, rs141074983) and three nucleotide substitutions in exon 3 (rs121908120, rs77583146, rs146902156). Mutational analysis of RUNX2 gene revealed four nucleotide substitutions in exon 3 (rs6921145, COSM1658842, rs759395776, rs768156797) and one in exon 4 (34 insC). Mutations in WNT10A gene were present in 52% of subjects, mutations in RUNX2 gene in 59% of subjects, mutations in both genes 35% of subjects, and no mutations were found in 24% of the sample..., Hipodoncija-urodjeni nedostatak zuba, predstavlja jednu od najĉešćih razvojnih anomalija kod ljudi, i moţe biti nesindromska i sindromska. Do razvoja hipodoncije moţe doći usled izmena u genima ukljuĉenim u morfogenezu zuba, a ĉiji produkti su signalni i transkripcioni faktori, odgovorni za vremenski programiranu ekspresiju gena u fazama pupoljka, kape i zvona. Iz palete gena ukljuĉenih u odontogenezu, zbog svog kljuĉnog znaĉaja izdvaja se WNT10A gen (eksprimiran u dentalnom epitelu), i RUNX2 gen (eksprimiran u mezenhimu). Mutacije gena povezanih sa odontogenezom mogu dovesti do izmena u morfologiji, broju i strukturi zuba. Ciljevi ove studije su bili da se ispita zastupljenost potencijalnih mutacija u RUNX2 i WNT10A genima i utvrdi njihov naĉin nasledjivanja kod izolovanih (nesindromskih) formi hipodoncije. Drugi deo istraţivanja odnosio se na uporedjivanje veliĉine zuba pacijenata sa urodjenim nedostatkom zuba i zdravih pacijenata kako bi se utvrdilo da li je hipodoncija/oligodoncija povezana sa eventualnim anomalijama veliĉine zuba . Mutacioni status RUNX2 i WNT10A gena, odreĊen je kod 24 porodice (72 ispitanika), pri ĉemu je kod 29 osoba, kliniĉki potvrdjena hipodoncija. Merenje meziodistalnih širina zuba je uradjeno kod 25 pacijenata sa uroĊenim nedostatkom zuba i 25 pacijenata kontrolne grupe. Prisustvo mutacija potvrĊeno je primenom tehnike direktnog sekvenciranja, dok je odreĊivanje meziodistalnih širina zuba uraĊeno primenom 3D skenera i merenjima na digitalnim modelima u kompjuterskom programu. Mutaciona analiza WNT10A gena, pokazala je prisustvo 2 nukleotidne izmene u regionu egzona 2 (rs10180544, rs141074983) i 3 izmene u egzonu 3 (rs121908120, rs77583146, rs146902156). Mutacionom analizom RUNX2 gena utvrĊeno je prisustvo 4 nukleotidne izmene u egzonu 3 (rs6921145, COSM1658842, rs759395776, rs768156797) i jedna nukleotidna izmena u regionu egzona 4 (34 insC)...",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "The influence of RUNX2 and WNT10A gene mutations on tooth number and size, Uticaj mutacija RUNX2 i WNT10A gena na broj i veličinu zuba",
url = "https://hdl.handle.net/21.15107/rcub_nardus_7723"
}
Živković-Sandić, M.. (2016). The influence of RUNX2 and WNT10A gene mutations on tooth number and size. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_7723
Živković-Sandić M. The influence of RUNX2 and WNT10A gene mutations on tooth number and size. 2016;.
https://hdl.handle.net/21.15107/rcub_nardus_7723 .
Živković-Sandić, Marija, "The influence of RUNX2 and WNT10A gene mutations on tooth number and size" (2016),
https://hdl.handle.net/21.15107/rcub_nardus_7723 .

History of medicine in Jagodina district

Jeremić, Marko; Vuković, Ana; Stanojlović, Ninoslav; Vuković, Rade; Marković, Dejan

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2015)

TY  - JOUR
AU  - Jeremić, Marko
AU  - Vuković, Ana
AU  - Stanojlović, Ninoslav
AU  - Vuković, Rade
AU  - Marković, Dejan
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2051
AB  - The first record of scientific medicine in Serbia has been found in the early of 12th century. For centuries lifestyle, nutrition, natural environment, armies passing through, cultural heritage, and prejudice have affected healthcare in Serbia. Until 1820, Serbia has not had any educated doctor. Fourteen district physicians from 1839 and Dr. Karlo Beloni, to the last one, Dr. Selimir Đorđević - have spent part of their professional careers in Jagodina. All of them have had influence on raising health culture of Jagodina and its population and helped to overcome easily and quickly all existing diseases and epidemics. The Jagodina Hospital has been working without interruption for 147 years and represents one of the oldest healthcare institutions in Serbia.
AB  - Prvi zapis o postojanju naučne medicine u Srbiji potiče iz ranog 12. veka. Stolećima je zdravstveno stanje srpskoga naroda bilo pod uticajem faktora kao što su način života, ishrana, prirodno okruženje, vojske koje prolaze, kulturno nasleđe i predrasude. Do 1820. godine u Srbiji nije bilo obrazovanih lekara. Četrnaest okružnih fizikusa od 1839. godine i dr Karla Belonija, do poslednjeg, dr Selimira Đorđevića, proveo je deo svoje profesionalne karijere u Jagodini. Svi oni su ostavili svoj humani i profesionalni trag na podizanju zdravstvene kulture Jagodine i njenog stanovništva, te pomogli lakše i brže prevazilaženje svih postojećih bolesti i epidemija. Bolnica u Jagodini radi bez prekida 147 godina i jedna je od najstarijih zdravstvenih ustanova u Srbiji.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - History of medicine in Jagodina district
T1  - Istorija medicine u Jagodinskom okrugu
VL  - 62
IS  - 4
SP  - 184
EP  - 195
DO  - 10.1515/sdj-2015-0019
ER  - 
@article{
author = "Jeremić, Marko and Vuković, Ana and Stanojlović, Ninoslav and Vuković, Rade and Marković, Dejan",
year = "2015",
abstract = "The first record of scientific medicine in Serbia has been found in the early of 12th century. For centuries lifestyle, nutrition, natural environment, armies passing through, cultural heritage, and prejudice have affected healthcare in Serbia. Until 1820, Serbia has not had any educated doctor. Fourteen district physicians from 1839 and Dr. Karlo Beloni, to the last one, Dr. Selimir Đorđević - have spent part of their professional careers in Jagodina. All of them have had influence on raising health culture of Jagodina and its population and helped to overcome easily and quickly all existing diseases and epidemics. The Jagodina Hospital has been working without interruption for 147 years and represents one of the oldest healthcare institutions in Serbia., Prvi zapis o postojanju naučne medicine u Srbiji potiče iz ranog 12. veka. Stolećima je zdravstveno stanje srpskoga naroda bilo pod uticajem faktora kao što su način života, ishrana, prirodno okruženje, vojske koje prolaze, kulturno nasleđe i predrasude. Do 1820. godine u Srbiji nije bilo obrazovanih lekara. Četrnaest okružnih fizikusa od 1839. godine i dr Karla Belonija, do poslednjeg, dr Selimira Đorđevića, proveo je deo svoje profesionalne karijere u Jagodini. Svi oni su ostavili svoj humani i profesionalni trag na podizanju zdravstvene kulture Jagodine i njenog stanovništva, te pomogli lakše i brže prevazilaženje svih postojećih bolesti i epidemija. Bolnica u Jagodini radi bez prekida 147 godina i jedna je od najstarijih zdravstvenih ustanova u Srbiji.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "History of medicine in Jagodina district, Istorija medicine u Jagodinskom okrugu",
volume = "62",
number = "4",
pages = "184-195",
doi = "10.1515/sdj-2015-0019"
}
Jeremić, M., Vuković, A., Stanojlović, N., Vuković, R.,& Marković, D.. (2015). History of medicine in Jagodina district. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 62(4), 184-195.
https://doi.org/10.1515/sdj-2015-0019
Jeremić M, Vuković A, Stanojlović N, Vuković R, Marković D. History of medicine in Jagodina district. in Stomatološki glasnik Srbije. 2015;62(4):184-195.
doi:10.1515/sdj-2015-0019 .
Jeremić, Marko, Vuković, Ana, Stanojlović, Ninoslav, Vuković, Rade, Marković, Dejan, "History of medicine in Jagodina district" in Stomatološki glasnik Srbije, 62, no. 4 (2015):184-195,
https://doi.org/10.1515/sdj-2015-0019 . .

Preserved insulin sensitivity predicts metabolically healthy obese phenotype in children and adolescents

Vuković, Rade; Milenković, Tatjana; Mitrović, Katarina; Todorović, Slađana; Plavsić, Ljiljana; Vuković, Ana; Zdravković, Dragan

(Springer, New York, 2015)

TY  - JOUR
AU  - Vuković, Rade
AU  - Milenković, Tatjana
AU  - Mitrović, Katarina
AU  - Todorović, Slađana
AU  - Plavsić, Ljiljana
AU  - Vuković, Ana
AU  - Zdravković, Dragan
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2010
AB  - Available data on metabolically healthy obese (MHO) phenotype in children suggest that gender, puberty, waist circumference, insulin sensitivity, and other laboratory predictors have a role in distinguishing these children from metabolically unhealthy obese (MUO) youth. The goal of this study was to identify predictors of MHO phenotype and to analyze glucose and insulin metabolism during oral glucose tolerance test (OGTT) in MHO children. OGTT was performed in 244 obese children and adolescents aged 4.6-18.9 years. Subjects were classified as MHO in case of no fulfilled criterion of metabolic syndrome except anthropometry or as MUO (a parts per thousand yen2 fulfilled criteria). Among the subjects, 21.7 % had MHO phenotype, and they were more likely to be female, younger, and in earlier stages of pubertal development, with lower degree of abdominal obesity. Insulin resistance was the only independent laboratory predictor of MUO phenotype (OR 1.59, CI 1.13-2.25), with 82 % sensitivity and 60 % specificity for diagnosing MUO using HOMA-IR cutoff point of a parts per thousand yen2.85. Although no significant differences were observed in glucose regulation, MUO children had higher insulin demand throughout OGTT, with 1.53 times higher total insulin secretion. Conclusion: Further research is needed to investigate the possibility of targeted treatment of insulin resistance to minimize pubertal cross-over to MUO in obese children.
PB  - Springer, New York
T2  - European Journal of Pediatrics
T1  - Preserved insulin sensitivity predicts metabolically healthy obese phenotype in children and adolescents
VL  - 174
IS  - 12
SP  - 1649
EP  - 1655
DO  - 10.1007/s00431-015-2587-4
ER  - 
@article{
author = "Vuković, Rade and Milenković, Tatjana and Mitrović, Katarina and Todorović, Slađana and Plavsić, Ljiljana and Vuković, Ana and Zdravković, Dragan",
year = "2015",
abstract = "Available data on metabolically healthy obese (MHO) phenotype in children suggest that gender, puberty, waist circumference, insulin sensitivity, and other laboratory predictors have a role in distinguishing these children from metabolically unhealthy obese (MUO) youth. The goal of this study was to identify predictors of MHO phenotype and to analyze glucose and insulin metabolism during oral glucose tolerance test (OGTT) in MHO children. OGTT was performed in 244 obese children and adolescents aged 4.6-18.9 years. Subjects were classified as MHO in case of no fulfilled criterion of metabolic syndrome except anthropometry or as MUO (a parts per thousand yen2 fulfilled criteria). Among the subjects, 21.7 % had MHO phenotype, and they were more likely to be female, younger, and in earlier stages of pubertal development, with lower degree of abdominal obesity. Insulin resistance was the only independent laboratory predictor of MUO phenotype (OR 1.59, CI 1.13-2.25), with 82 % sensitivity and 60 % specificity for diagnosing MUO using HOMA-IR cutoff point of a parts per thousand yen2.85. Although no significant differences were observed in glucose regulation, MUO children had higher insulin demand throughout OGTT, with 1.53 times higher total insulin secretion. Conclusion: Further research is needed to investigate the possibility of targeted treatment of insulin resistance to minimize pubertal cross-over to MUO in obese children.",
publisher = "Springer, New York",
journal = "European Journal of Pediatrics",
title = "Preserved insulin sensitivity predicts metabolically healthy obese phenotype in children and adolescents",
volume = "174",
number = "12",
pages = "1649-1655",
doi = "10.1007/s00431-015-2587-4"
}
Vuković, R., Milenković, T., Mitrović, K., Todorović, S., Plavsić, L., Vuković, A.,& Zdravković, D.. (2015). Preserved insulin sensitivity predicts metabolically healthy obese phenotype in children and adolescents. in European Journal of Pediatrics
Springer, New York., 174(12), 1649-1655.
https://doi.org/10.1007/s00431-015-2587-4
Vuković R, Milenković T, Mitrović K, Todorović S, Plavsić L, Vuković A, Zdravković D. Preserved insulin sensitivity predicts metabolically healthy obese phenotype in children and adolescents. in European Journal of Pediatrics. 2015;174(12):1649-1655.
doi:10.1007/s00431-015-2587-4 .
Vuković, Rade, Milenković, Tatjana, Mitrović, Katarina, Todorović, Slađana, Plavsić, Ljiljana, Vuković, Ana, Zdravković, Dragan, "Preserved insulin sensitivity predicts metabolically healthy obese phenotype in children and adolescents" in European Journal of Pediatrics, 174, no. 12 (2015):1649-1655,
https://doi.org/10.1007/s00431-015-2587-4 . .
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