Importance of viral genetic variability in human diseases

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Importance of viral genetic variability in human diseases (en)
Медицински значај генетичке варијабилности вируса (sr)
Medicinski značaj genetičke varijabilnosti virusa (sr_RS)
Authors

Publications

Levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by Epstein-Barr virus

Jakovljević, Aleksandar; Andrić, Miroslav; Nikolić, Nadja; Corić, V.; Krezović, S.; Čarkić, Jelena; Knežević, Aleksandra; Beljić-Ivanović, Katarina; Pljesa-Ercegovac, M.; Miletić, Maja; Soldatović, Ivan; Radosavljević, T.; Jovanović, T.; Simić, T.; Ivanović, Vladimir; Milašin, Jelena

(Wiley, Hoboken, 2018)

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Andrić, Miroslav
AU  - Nikolić, Nadja
AU  - Corić, V.
AU  - Krezović, S.
AU  - Čarkić, Jelena
AU  - Knežević, Aleksandra
AU  - Beljić-Ivanović, Katarina
AU  - Pljesa-Ercegovac, M.
AU  - Miletić, Maja
AU  - Soldatović, Ivan
AU  - Radosavljević, T.
AU  - Jovanović, T.
AU  - Simić, T.
AU  - Ivanović, Vladimir
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2293
AB  - AimTo investigate whether apical periodontitis lesions infected by Epstein-Barr virus (EBV) exhibit higher levels of oxidative stress biomarkers [8-hydroxydeoxyguanosine (8-OHdG) and oxidized glutathione (GSSG)] and bone resorption regulators [receptor activator of nuclear factor (NF-B) ligand (RANKL) and osteoprotegerin (OPG)] compared to EBV-negative periapical lesions and healthy pulp tissues. MethodologyThe experimental group consisted of 30 EBV-positive and 30 EBV-negative periapical lesions collected in conjunction with apicoectomy. The pulp tissues of 20 impacted third molars were used as healthy controls. The qualitative and quantitative analysis of EBV was performed by nested and real-time polymerase chain reaction (PCR), respectively. The levels of RANKL and OPG were analysed by reverse transcriptase real-time PCR. The levels of 8-OHdG and GSSG were determined by enzyme-linked immunosorbent assay (ELISA). Mann-Whitney U-test and Spearman's correlation were used for statistical analysis. ResultsThe levels of RANKL, OPG, 8-OHdG and GSSG were significantly higher in apical periodontitis lesions compared to healthy pulp controls (P=0.001, P lt 0.001, P lt 0.001 and P lt 0.05, respectively). RANKL and OPG mRNA expression was significantly higher in EBV-positive compared to EBV-negative periapical lesions (P lt 0.05). There was no significant correlation between EBV copy numbers and levels of RANKL, OPG, 8OH-dG and GSSG in apical periodontitis. ConclusionLevels of bone resorption regulators and oxidative stress biomarkers were increased in apical periodontitis compared to healthy pulp tissues. EBV-positive periapical lesions exhibited higher levels of RANKL and OPG compared to EBV-negative periapical lesions. EBV may contribute to progression of apical periodontitis via enhanced production of bone resorption regulators.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by Epstein-Barr virus
VL  - 51
IS  - 6
SP  - 593
EP  - 604
DO  - 10.1111/iej.12886
ER  - 
@article{
author = "Jakovljević, Aleksandar and Andrić, Miroslav and Nikolić, Nadja and Corić, V. and Krezović, S. and Čarkić, Jelena and Knežević, Aleksandra and Beljić-Ivanović, Katarina and Pljesa-Ercegovac, M. and Miletić, Maja and Soldatović, Ivan and Radosavljević, T. and Jovanović, T. and Simić, T. and Ivanović, Vladimir and Milašin, Jelena",
year = "2018",
abstract = "AimTo investigate whether apical periodontitis lesions infected by Epstein-Barr virus (EBV) exhibit higher levels of oxidative stress biomarkers [8-hydroxydeoxyguanosine (8-OHdG) and oxidized glutathione (GSSG)] and bone resorption regulators [receptor activator of nuclear factor (NF-B) ligand (RANKL) and osteoprotegerin (OPG)] compared to EBV-negative periapical lesions and healthy pulp tissues. MethodologyThe experimental group consisted of 30 EBV-positive and 30 EBV-negative periapical lesions collected in conjunction with apicoectomy. The pulp tissues of 20 impacted third molars were used as healthy controls. The qualitative and quantitative analysis of EBV was performed by nested and real-time polymerase chain reaction (PCR), respectively. The levels of RANKL and OPG were analysed by reverse transcriptase real-time PCR. The levels of 8-OHdG and GSSG were determined by enzyme-linked immunosorbent assay (ELISA). Mann-Whitney U-test and Spearman's correlation were used for statistical analysis. ResultsThe levels of RANKL, OPG, 8-OHdG and GSSG were significantly higher in apical periodontitis lesions compared to healthy pulp controls (P=0.001, P lt 0.001, P lt 0.001 and P lt 0.05, respectively). RANKL and OPG mRNA expression was significantly higher in EBV-positive compared to EBV-negative periapical lesions (P lt 0.05). There was no significant correlation between EBV copy numbers and levels of RANKL, OPG, 8OH-dG and GSSG in apical periodontitis. ConclusionLevels of bone resorption regulators and oxidative stress biomarkers were increased in apical periodontitis compared to healthy pulp tissues. EBV-positive periapical lesions exhibited higher levels of RANKL and OPG compared to EBV-negative periapical lesions. EBV may contribute to progression of apical periodontitis via enhanced production of bone resorption regulators.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by Epstein-Barr virus",
volume = "51",
number = "6",
pages = "593-604",
doi = "10.1111/iej.12886"
}
Jakovljević, A., Andrić, M., Nikolić, N., Corić, V., Krezović, S., Čarkić, J., Knežević, A., Beljić-Ivanović, K., Pljesa-Ercegovac, M., Miletić, M., Soldatović, I., Radosavljević, T., Jovanović, T., Simić, T., Ivanović, V.,& Milašin, J.. (2018). Levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by Epstein-Barr virus. in International Endodontic Journal
Wiley, Hoboken., 51(6), 593-604.
https://doi.org/10.1111/iej.12886
Jakovljević A, Andrić M, Nikolić N, Corić V, Krezović S, Čarkić J, Knežević A, Beljić-Ivanović K, Pljesa-Ercegovac M, Miletić M, Soldatović I, Radosavljević T, Jovanović T, Simić T, Ivanović V, Milašin J. Levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by Epstein-Barr virus. in International Endodontic Journal. 2018;51(6):593-604.
doi:10.1111/iej.12886 .
Jakovljević, Aleksandar, Andrić, Miroslav, Nikolić, Nadja, Corić, V., Krezović, S., Čarkić, Jelena, Knežević, Aleksandra, Beljić-Ivanović, Katarina, Pljesa-Ercegovac, M., Miletić, Maja, Soldatović, Ivan, Radosavljević, T., Jovanović, T., Simić, T., Ivanović, Vladimir, Milašin, Jelena, "Levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by Epstein-Barr virus" in International Endodontic Journal, 51, no. 6 (2018):593-604,
https://doi.org/10.1111/iej.12886 . .
16
13
16

Detection and genotypisation of human cytomegalovirus and epstein-barr virus in chronic periapical lesions.

Jakovljević, Aleksandar

(Univerzitet u Beogradu, Stomatološki fakultet, 2017)

TY  - THES
AU  - Jakovljević, Aleksandar
PY  - 2017
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=5173
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:16039/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=49209359
UR  - http://nardus.mpn.gov.rs/123456789/8451
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1024
AB  - Periapical lesions are characterized by chronic inflammation and destruction of tooth-supporting tissues in the periapical area of non-vital teeth. Previous studies confirmed that polymicrobial infection of endodontic origin is essential for its development. At the beginning of 21st century herpesviruses have been detected in tissue samples of periapical lesions. It has been hypothesized that concomitant herpesviral infection may contribute to the pathogenesis of apical periodontitis in the same way as it has been proposed for marginal periodontitis. Up to now, 20 studies investigated the occurrence of herpesviral infection in apical periodontitis. Their results indicate that Epstein-Barr virus (EBV) and Human cytomegalovirus (HCMV) are the most frequently detected species of Herpesviridae family in apical periodontitis. Previous studies showed that herpesviruses occurred more frequently in symptomatic and large compared to asymptomatic and small periapical lesions. However, their exact role in the pathogenesis of apical periodontitis has not been fully elucidated. Although it is considered that a great diversity of viral strains may influence the course and severity of disease there are no scientific data about the occurrence of different EBV and HCMV genotypes in apical periodontitis samples. Furthermore, there is a lack of data about the relationship between levels of pro-inflammatory cytokines and herpesviral copy number in the pathogenesis of apical periodontitis. AIMS. This study aimed to detect the occurrence of HCMV and EBV, to assess their copy number and the distribution of their genotypes in periapical lesions and healthy control tissues. Additionally, this study aimed to compare these findings between symptomatic and asymptomatic, large and small periapical lesions, and periapical granulomas and radicular cysts. The goal of the study was also to determine the levels of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) in periapical lesions and healthy control tissues, and to correlate these data with the copy number of investigated herpesviruses...
AB  - Periapikalne lezije karakteriše hronična zapaljenska reakcija koja uzrokuje razgradnju alveolarnog koštanog tkiva u predelu vrha korena avitalnih zuba. Dosadašnja istraživanja pokazuju da su bakterijski mikroorganizmi i njihovi štetni produkti najčešći uzročnici nastanka zapaljenske reakcije u apeksnom parodoncijumu. Početkom 21. veka dokazano je prisustvo herpesvirusa u tkivu periapikalnih lezija i postavljena je hipoteza da pomenuti virusi učestvuju u procesu njihovog nastanka po modelu kojim doprinose razgradnji alveolarnog koštanog tkiva, kao i u marginalnom parodontitisu. Do danas je publikovano 20 istraživanja koja su ispitivala učestalost herpesvirusne infekcije u tkivu periapikalnih lezija. Rezultati istraživanja ukazuju da su Epštajn-Bar virus (EBV) i Humani citomegalovirus (HCMV) najčešće detektovani pripadnici porodice Herpesviridae u periapikalnim lezijama. Pojedina istraživanja su pokazala da su herpesvirusi češće detektovani u simptomatskim i velikim u odnosu na asimptomatske i male lezije. Međutim, njihova uloga u patogenezi periapikalnih lezija još uvek nije u potpunosti razjašnjena. Iako se smatra da genetička varijabilnost herpesvirusa može uticati na odnos virusa i imunskog odgovor domaćina, u naučnoj literaturi još uvek nema podataka o učestalosti pojedinih genotipova EBV i HCMV u periapikalnim lezijama. Takođe, nedovoljno je ispitan odnos povećanog broja genskih kopija herpesvirusa i nivoa proinflamatornih citokina u patogenezi periapikalnih lezija. CILJ ISTRAŽIVANJA. Ovo istraživanje je imalo za cilj da odredi učestalost prisustva, broja genskih kopija i vrste genotipova EBV i HCMV u tkivu periapikalnih lezija i kontrolnog pulpnog tkiva, kao i da uporedi dobijeni nalaz među periapikalnim lezijama sa različitim kliničkim, radiološkim i patohistološkim karakteristikama. Takođe, cilj ovog istraživanja bio je i da se odredi vrednost proinflamatornih citokina (TNF-α, IL-1β i IL-6) u tkivu periapikalnih lezija i kontrolnog pulpnog tkiva, te da se dobijene vrednosti uporede sa brojem genskih kopija EBV i HCMV u ispitivanom uzorku. MATERIJAL I METOD. U istraživanju je analizirano 100 uzoraka hroničnih periapikalnih lezija i 25 uzoraka kontrolnog pulpnog tkiva. Uzorci su prikupljani od zdravih pacijenata kod kojih je indikovana resekcija vrha korena zuba ili hirurško vađenje imapaktiranih umnjaka...
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - Detection and genotypisation of human cytomegalovirus and epstein-barr virus in chronic periapical lesions.
T1  - Detekcija i genotipizacija humanog citomegalovirusa i Epštajn-Bar virusa u hroničnim periapikalnim lezijama
UR  - https://hdl.handle.net/21.15107/rcub_nardus_8451
ER  - 
@phdthesis{
author = "Jakovljević, Aleksandar",
year = "2017",
abstract = "Periapical lesions are characterized by chronic inflammation and destruction of tooth-supporting tissues in the periapical area of non-vital teeth. Previous studies confirmed that polymicrobial infection of endodontic origin is essential for its development. At the beginning of 21st century herpesviruses have been detected in tissue samples of periapical lesions. It has been hypothesized that concomitant herpesviral infection may contribute to the pathogenesis of apical periodontitis in the same way as it has been proposed for marginal periodontitis. Up to now, 20 studies investigated the occurrence of herpesviral infection in apical periodontitis. Their results indicate that Epstein-Barr virus (EBV) and Human cytomegalovirus (HCMV) are the most frequently detected species of Herpesviridae family in apical periodontitis. Previous studies showed that herpesviruses occurred more frequently in symptomatic and large compared to asymptomatic and small periapical lesions. However, their exact role in the pathogenesis of apical periodontitis has not been fully elucidated. Although it is considered that a great diversity of viral strains may influence the course and severity of disease there are no scientific data about the occurrence of different EBV and HCMV genotypes in apical periodontitis samples. Furthermore, there is a lack of data about the relationship between levels of pro-inflammatory cytokines and herpesviral copy number in the pathogenesis of apical periodontitis. AIMS. This study aimed to detect the occurrence of HCMV and EBV, to assess their copy number and the distribution of their genotypes in periapical lesions and healthy control tissues. Additionally, this study aimed to compare these findings between symptomatic and asymptomatic, large and small periapical lesions, and periapical granulomas and radicular cysts. The goal of the study was also to determine the levels of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) in periapical lesions and healthy control tissues, and to correlate these data with the copy number of investigated herpesviruses..., Periapikalne lezije karakteriše hronična zapaljenska reakcija koja uzrokuje razgradnju alveolarnog koštanog tkiva u predelu vrha korena avitalnih zuba. Dosadašnja istraživanja pokazuju da su bakterijski mikroorganizmi i njihovi štetni produkti najčešći uzročnici nastanka zapaljenske reakcije u apeksnom parodoncijumu. Početkom 21. veka dokazano je prisustvo herpesvirusa u tkivu periapikalnih lezija i postavljena je hipoteza da pomenuti virusi učestvuju u procesu njihovog nastanka po modelu kojim doprinose razgradnji alveolarnog koštanog tkiva, kao i u marginalnom parodontitisu. Do danas je publikovano 20 istraživanja koja su ispitivala učestalost herpesvirusne infekcije u tkivu periapikalnih lezija. Rezultati istraživanja ukazuju da su Epštajn-Bar virus (EBV) i Humani citomegalovirus (HCMV) najčešće detektovani pripadnici porodice Herpesviridae u periapikalnim lezijama. Pojedina istraživanja su pokazala da su herpesvirusi češće detektovani u simptomatskim i velikim u odnosu na asimptomatske i male lezije. Međutim, njihova uloga u patogenezi periapikalnih lezija još uvek nije u potpunosti razjašnjena. Iako se smatra da genetička varijabilnost herpesvirusa može uticati na odnos virusa i imunskog odgovor domaćina, u naučnoj literaturi još uvek nema podataka o učestalosti pojedinih genotipova EBV i HCMV u periapikalnim lezijama. Takođe, nedovoljno je ispitan odnos povećanog broja genskih kopija herpesvirusa i nivoa proinflamatornih citokina u patogenezi periapikalnih lezija. CILJ ISTRAŽIVANJA. Ovo istraživanje je imalo za cilj da odredi učestalost prisustva, broja genskih kopija i vrste genotipova EBV i HCMV u tkivu periapikalnih lezija i kontrolnog pulpnog tkiva, kao i da uporedi dobijeni nalaz među periapikalnim lezijama sa različitim kliničkim, radiološkim i patohistološkim karakteristikama. Takođe, cilj ovog istraživanja bio je i da se odredi vrednost proinflamatornih citokina (TNF-α, IL-1β i IL-6) u tkivu periapikalnih lezija i kontrolnog pulpnog tkiva, te da se dobijene vrednosti uporede sa brojem genskih kopija EBV i HCMV u ispitivanom uzorku. MATERIJAL I METOD. U istraživanju je analizirano 100 uzoraka hroničnih periapikalnih lezija i 25 uzoraka kontrolnog pulpnog tkiva. Uzorci su prikupljani od zdravih pacijenata kod kojih je indikovana resekcija vrha korena zuba ili hirurško vađenje imapaktiranih umnjaka...",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "Detection and genotypisation of human cytomegalovirus and epstein-barr virus in chronic periapical lesions., Detekcija i genotipizacija humanog citomegalovirusa i Epštajn-Bar virusa u hroničnim periapikalnim lezijama",
url = "https://hdl.handle.net/21.15107/rcub_nardus_8451"
}
Jakovljević, A.. (2017). Detection and genotypisation of human cytomegalovirus and epstein-barr virus in chronic periapical lesions.. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_8451
Jakovljević A. Detection and genotypisation of human cytomegalovirus and epstein-barr virus in chronic periapical lesions.. 2017;.
https://hdl.handle.net/21.15107/rcub_nardus_8451 .
Jakovljević, Aleksandar, "Detection and genotypisation of human cytomegalovirus and epstein-barr virus in chronic periapical lesions." (2017),
https://hdl.handle.net/21.15107/rcub_nardus_8451 .

Herpesviruses in Periapical Pathoses: An Updated Systematic Review

Ongrádi, Joseph; Jakovljević, Aleksandar; Andrić, Miroslav; Knežević, Aleksandra; Beljić-Ivanović, Katarina; Miletić, Maja; Jovanović, Tanja; Kesić, Ljiljana; Milasin, Jelena

(Rijeka : InTech, 2016)

TY  - CHAP
AU  - Jakovljević, Aleksandar
AU  - Andrić, Miroslav
AU  - Knežević, Aleksandra
AU  - Beljić-Ivanović, Katarina
AU  - Miletić, Maja
AU  - Jovanović, Tanja
AU  - Kesić, Ljiljana
AU  - Milasin, Jelena
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2597
AB  - Apical periodontitis represents a chronic inflammation and destruction of periradicular tissue caused by polymicrobial infection of endodontic origin. The aim of this systematic review was to make an update on findings related to Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) presence in periapical pathoses and to correlate these findings with clinical, histopathological and radiographic features of periapical lesions. Methods were based on the preferred reporting items for systematic reviews and meta-analyses (PRISMA) statement. A search was performed using PubMed, Web of Science and SCOPUS. Search key words included the following medical subjects heading terms: (periapical disease OR apical periodontitis OR periapical lesions OR periapical abscess) AND (viruses OR herpesvir*). A manual search involved references from articles retrieved for possible inclusion. The search, evalua‐ tion, and critical appraisal of articles were performed by two independent judges. Collected data were analyzed using the measures of descriptive statistics. The final review has included twenty nine articles related to herpesviral presence periapical pathoses. Qualitative analysis indicated that EBV HCMV, and HHV-8 were the most prevalent species in periapical pathoses. Our findings suggest that there is wide variety of herpesviruses detection rates in periapical pathoses in relation to their clinical, histopathological and radiographic features.
PB  - Rijeka : InTech
T2  - Herpesviridae
T1  - Herpesviruses in Periapical Pathoses: An Updated Systematic Review
SP  - 31
EP  - 74
DO  - 10.5772/64004
ER  - 
@inbook{
editor = "Ongrádi, Joseph",
author = "Jakovljević, Aleksandar and Andrić, Miroslav and Knežević, Aleksandra and Beljić-Ivanović, Katarina and Miletić, Maja and Jovanović, Tanja and Kesić, Ljiljana and Milasin, Jelena",
year = "2016",
abstract = "Apical periodontitis represents a chronic inflammation and destruction of periradicular tissue caused by polymicrobial infection of endodontic origin. The aim of this systematic review was to make an update on findings related to Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) presence in periapical pathoses and to correlate these findings with clinical, histopathological and radiographic features of periapical lesions. Methods were based on the preferred reporting items for systematic reviews and meta-analyses (PRISMA) statement. A search was performed using PubMed, Web of Science and SCOPUS. Search key words included the following medical subjects heading terms: (periapical disease OR apical periodontitis OR periapical lesions OR periapical abscess) AND (viruses OR herpesvir*). A manual search involved references from articles retrieved for possible inclusion. The search, evalua‐ tion, and critical appraisal of articles were performed by two independent judges. Collected data were analyzed using the measures of descriptive statistics. The final review has included twenty nine articles related to herpesviral presence periapical pathoses. Qualitative analysis indicated that EBV HCMV, and HHV-8 were the most prevalent species in periapical pathoses. Our findings suggest that there is wide variety of herpesviruses detection rates in periapical pathoses in relation to their clinical, histopathological and radiographic features.",
publisher = "Rijeka : InTech",
journal = "Herpesviridae",
booktitle = "Herpesviruses in Periapical Pathoses: An Updated Systematic Review",
pages = "31-74",
doi = "10.5772/64004"
}
Ongrádi, J., Jakovljević, A., Andrić, M., Knežević, A., Beljić-Ivanović, K., Miletić, M., Jovanović, T., Kesić, L.,& Milasin, J.. (2016). Herpesviruses in Periapical Pathoses: An Updated Systematic Review. in Herpesviridae
Rijeka : InTech., 31-74.
https://doi.org/10.5772/64004
Ongrádi J, Jakovljević A, Andrić M, Knežević A, Beljić-Ivanović K, Miletić M, Jovanović T, Kesić L, Milasin J. Herpesviruses in Periapical Pathoses: An Updated Systematic Review. in Herpesviridae. 2016;:31-74.
doi:10.5772/64004 .
Ongrádi, Joseph, Jakovljević, Aleksandar, Andrić, Miroslav, Knežević, Aleksandra, Beljić-Ivanović, Katarina, Miletić, Maja, Jovanović, Tanja, Kesić, Ljiljana, Milasin, Jelena, "Herpesviruses in Periapical Pathoses: An Updated Systematic Review" in Herpesviridae (2016):31-74,
https://doi.org/10.5772/64004 . .
3

Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions

Jakovljević, Aleksandar; Andrić, Miroslav; Knežević, Aleksandra; Soldatović, Ivan; Nikolić, Nadja; Karalić, Danijela; Milašin, Jelena

(Elsevier Science Inc, New York, 2015)

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Andrić, Miroslav
AU  - Knežević, Aleksandra
AU  - Soldatović, Ivan
AU  - Nikolić, Nadja
AU  - Karalić, Danijela
AU  - Milašin, Jelena
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1996
AB  - Introduction: Different genotypes of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) possess specific pathogenic abilities because of various interactions with the host's immune system and differences in cell tropism. The aim of this study was to determine the distribution of HCMV and EBV genotypes in apical periodontitis lesions in relation to their clinical and histopathologic features. Methods: One hundred samples of apical periodontitis lesions and 25 control samples (healthy pulp tissue) were collected. The presence of HCMV glycoprotein B (gB) and EBV nuclear antigen-2 genotypes was analyzed by nested polymerase chain reaction and restriction fragment length polymorphisms analysis. Results: EBV and HCMV were detected in apical periodontitis lesions at significantly higher frequencies than in healthy pulp controls (P = .020 and P = .020, respectively). HCMV gB type II was significantly more frequent compared with gB type I in the examined groups (P = .036). No HCMV gB type III or IV products were found. In both periapical lesions and controls, EBV-1 occurred more often compared with EBV-2 (P = .001). Dual EBV and HCMV coinfection was more frequently detected in large-size periapical lesions (P = .038). Conclusions: Both HCMV and EBV are associated with inflammatory processes of periapical bone destruction. HCMV gB type II and EBV-1 are the most prevalent genotypes in apical periodontitis lesions.
PB  - Elsevier Science Inc, New York
T2  - Journal of Endodontics
T1  - Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions
VL  - 41
IS  - 11
SP  - 1847
EP  - 1851
DO  - 10.1016/j.joen.2015.08.027
ER  - 
@article{
author = "Jakovljević, Aleksandar and Andrić, Miroslav and Knežević, Aleksandra and Soldatović, Ivan and Nikolić, Nadja and Karalić, Danijela and Milašin, Jelena",
year = "2015",
abstract = "Introduction: Different genotypes of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) possess specific pathogenic abilities because of various interactions with the host's immune system and differences in cell tropism. The aim of this study was to determine the distribution of HCMV and EBV genotypes in apical periodontitis lesions in relation to their clinical and histopathologic features. Methods: One hundred samples of apical periodontitis lesions and 25 control samples (healthy pulp tissue) were collected. The presence of HCMV glycoprotein B (gB) and EBV nuclear antigen-2 genotypes was analyzed by nested polymerase chain reaction and restriction fragment length polymorphisms analysis. Results: EBV and HCMV were detected in apical periodontitis lesions at significantly higher frequencies than in healthy pulp controls (P = .020 and P = .020, respectively). HCMV gB type II was significantly more frequent compared with gB type I in the examined groups (P = .036). No HCMV gB type III or IV products were found. In both periapical lesions and controls, EBV-1 occurred more often compared with EBV-2 (P = .001). Dual EBV and HCMV coinfection was more frequently detected in large-size periapical lesions (P = .038). Conclusions: Both HCMV and EBV are associated with inflammatory processes of periapical bone destruction. HCMV gB type II and EBV-1 are the most prevalent genotypes in apical periodontitis lesions.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Endodontics",
title = "Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions",
volume = "41",
number = "11",
pages = "1847-1851",
doi = "10.1016/j.joen.2015.08.027"
}
Jakovljević, A., Andrić, M., Knežević, A., Soldatović, I., Nikolić, N., Karalić, D.,& Milašin, J.. (2015). Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions. in Journal of Endodontics
Elsevier Science Inc, New York., 41(11), 1847-1851.
https://doi.org/10.1016/j.joen.2015.08.027
Jakovljević A, Andrić M, Knežević A, Soldatović I, Nikolić N, Karalić D, Milašin J. Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions. in Journal of Endodontics. 2015;41(11):1847-1851.
doi:10.1016/j.joen.2015.08.027 .
Jakovljević, Aleksandar, Andrić, Miroslav, Knežević, Aleksandra, Soldatović, Ivan, Nikolić, Nadja, Karalić, Danijela, Milašin, Jelena, "Human Cytomegalovirus and Epstein-Barr Virus Genotypes in Apical Periodontitis Lesions" in Journal of Endodontics, 41, no. 11 (2015):1847-1851,
https://doi.org/10.1016/j.joen.2015.08.027 . .
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Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings

Jakovljević, Aleksandar; Knežević, Aleksandra; Karalić, Danijela; Soldatović, Ivan; Popović, Branka; Milašin, Jelena; Andrić, Miroslav

(Wiley, Hoboken, 2015)

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Knežević, Aleksandra
AU  - Karalić, Danijela
AU  - Soldatović, Ivan
AU  - Popović, Branka
AU  - Milašin, Jelena
AU  - Andrić, Miroslav
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1986
AB  - This study aimed to compare the levels of tumour necrosis factor-alpha (TNF-), interleukin-1 beta (IL-1) and interleukin-6 (IL-6) between apical periodontitis lesions with different clinical and histological features. Based on clinical data and history of disease, 100 human apical periodontitis lesions were categorised as either asymptomatic or symptomatic lesions. According to histological examination, lesions were divided into periapical granulomas and radicular cysts. Pulp tissues of 25 impacted wisdom teeth were used as controls. Homogenised tissue samples were centrifuged and supernatants were used for the determination of cytokine levels by enzyme-linked immunosorbent assay. Significantly higher levels of IL-1 and IL-6 were found in symptomatic lesions compared with asymptomatic lesions and control tissues (P lt 0.001, P lt 0.001, respectively). The concentration of IL-1 was significantly higher in radicular cysts compared with periapical granulomas (P=0.003). Symptomatic lesions, as judged by high local production of IL-1 and IL-6, represent an immunologically active stage of the disease.
PB  - Wiley, Hoboken
T2  - Australian Endodontic Journal
T1  - Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings
VL  - 41
IS  - 2
SP  - 72
EP  - 77
DO  - 10.1111/aej.12072
ER  - 
@article{
author = "Jakovljević, Aleksandar and Knežević, Aleksandra and Karalić, Danijela and Soldatović, Ivan and Popović, Branka and Milašin, Jelena and Andrić, Miroslav",
year = "2015",
abstract = "This study aimed to compare the levels of tumour necrosis factor-alpha (TNF-), interleukin-1 beta (IL-1) and interleukin-6 (IL-6) between apical periodontitis lesions with different clinical and histological features. Based on clinical data and history of disease, 100 human apical periodontitis lesions were categorised as either asymptomatic or symptomatic lesions. According to histological examination, lesions were divided into periapical granulomas and radicular cysts. Pulp tissues of 25 impacted wisdom teeth were used as controls. Homogenised tissue samples were centrifuged and supernatants were used for the determination of cytokine levels by enzyme-linked immunosorbent assay. Significantly higher levels of IL-1 and IL-6 were found in symptomatic lesions compared with asymptomatic lesions and control tissues (P lt 0.001, P lt 0.001, respectively). The concentration of IL-1 was significantly higher in radicular cysts compared with periapical granulomas (P=0.003). Symptomatic lesions, as judged by high local production of IL-1 and IL-6, represent an immunologically active stage of the disease.",
publisher = "Wiley, Hoboken",
journal = "Australian Endodontic Journal",
title = "Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings",
volume = "41",
number = "2",
pages = "72-77",
doi = "10.1111/aej.12072"
}
Jakovljević, A., Knežević, A., Karalić, D., Soldatović, I., Popović, B., Milašin, J.,& Andrić, M.. (2015). Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings. in Australian Endodontic Journal
Wiley, Hoboken., 41(2), 72-77.
https://doi.org/10.1111/aej.12072
Jakovljević A, Knežević A, Karalić D, Soldatović I, Popović B, Milašin J, Andrić M. Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings. in Australian Endodontic Journal. 2015;41(2):72-77.
doi:10.1111/aej.12072 .
Jakovljević, Aleksandar, Knežević, Aleksandra, Karalić, Danijela, Soldatović, Ivan, Popović, Branka, Milašin, Jelena, Andrić, Miroslav, "Pro-inflammatory cytokine levels in human apical periodontitis: Correlation with clinical and histological findings" in Australian Endodontic Journal, 41, no. 2 (2015):72-77,
https://doi.org/10.1111/aej.12072 . .
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