TY  - JOUR
AU  - Kanjevac, Tatjana
AU  - Milovanović, Marija
AU  - Milošević-Đorđević, Olivera
AU  - Tešić, Živoslav
AU  - Ivanović, Mirjana
AU  - Lukić, Aleksandra
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1968
AB  - Stem cells from human exfoliated deciduous teeth (SHED) can be used as a cell-based therapy in regenerative medicine and in immunomodulation. Pulp from human deciduous teeth can be stored as a source of SHED. Glass ionomer cements (GICs) are commonly used in restorative dentistry and in cavity lining. GICs have lower biocompatibility and are cytotoxic for dental pulp cells. In this study, seven commonly used GICs were tested for their cytotoxic effects on SHED, for their potential to arrest mitosis in cells and induce chromosome aberrations, and were compared with the effects of composite. Fuji II, Fuji VIII, Fuji IX, Fuji plus and Vitrebond had significantly higher cytotoxic effects on SHED than composite. Only SHEDs that have been treated with Fuji I, Fuji IX, Fuji plus and composite recovered the potential for proliferation, but no chromosome aberrations were found after treatment with GICs. The cytotoxic effects of GICs on SHEDs were in strong correlation with combined concentrations of released fluoride, aluminum and strontium ions. Fuji I exhibited the lowest activity towards SHEDs; it did not interrupt mitosis and did not induce chromosome aberrations, and was accompanied by the lowest levels of released F, Al and Sr ions.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrations
VL  - 67
IS  - 2
SP  - 619
EP  - 630
DO  - 10.2298/ABS141021022K
ER  - 

@article{
author = "Kanjevac, Tatjana and Milovanović, Marija and Milošević-Đorđević, Olivera and Tešić, Živoslav and Ivanović, Mirjana and Lukić, Aleksandra",
year = "2015",
abstract = "Stem cells from human exfoliated deciduous teeth (SHED) can be used as a cell-based therapy in regenerative medicine and in immunomodulation. Pulp from human deciduous teeth can be stored as a source of SHED. Glass ionomer cements (GICs) are commonly used in restorative dentistry and in cavity lining. GICs have lower biocompatibility and are cytotoxic for dental pulp cells. In this study, seven commonly used GICs were tested for their cytotoxic effects on SHED, for their potential to arrest mitosis in cells and induce chromosome aberrations, and were compared with the effects of composite. Fuji II, Fuji VIII, Fuji IX, Fuji plus and Vitrebond had significantly higher cytotoxic effects on SHED than composite. Only SHEDs that have been treated with Fuji I, Fuji IX, Fuji plus and composite recovered the potential for proliferation, but no chromosome aberrations were found after treatment with GICs. The cytotoxic effects of GICs on SHEDs were in strong correlation with combined concentrations of released fluoride, aluminum and strontium ions. Fuji I exhibited the lowest activity towards SHEDs; it did not interrupt mitosis and did not induce chromosome aberrations, and was accompanied by the lowest levels of released F, Al and Sr ions.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrations",
volume = "67",
number = "2",
pages = "619-630",
doi = "10.2298/ABS141021022K"
}

Kanjevac, T., Milovanović, M., Milošević-Đorđević, O., Tešić, Ž., Ivanović, M.,& Lukić, A.. (2015). Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrations. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 67(2), 619-630.
https://doi.org/10.2298/ABS141021022K

Kanjevac T, Milovanović M, Milošević-Đorđević O, Tešić Ž, Ivanović M, Lukić A. Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrations. in Archives of Biological Sciences. 2015;67(2):619-630.
doi:10.2298/ABS141021022K .

Kanjevac, Tatjana, Milovanović, Marija, Milošević-Đorđević, Olivera, Tešić, Živoslav, Ivanović, Mirjana, Lukić, Aleksandra, "Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrations" in Archives of Biological Sciences, 67, no. 2 (2015):619-630,
https://doi.org/10.2298/ABS141021022K . .

TY  - JOUR
AU  - Kanjevac, Tatjana
AU  - Milovanović, Marija
AU  - Volarević, Vladislav
AU  - Lukić, Miodrag L.
AU  - Arsenijević, Nebojša
AU  - Marković, Dejan
AU  - Zdravković, Nebojša
AU  - Tešić, Živoslav
AU  - Lukić, Aleksandra
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1752
AB  - Objectives: Glass ionomer cements (GICs) are commonly used as restorative materials. Responses to GICs differ among cell types and it is therefore of importance to thoroughly investigate the influence of these restorative materials on pulp stem cells that are potential source for dental tissue regeneration. Eight biomaterials were tested: Fuji I, Fuji II, Fuji VIII, Fuji IX, Fuji Plus, Fuji Triage, Vitrebond and Composit. We compared their cytotoxic activity on human dental pulp stem cells (DPSC) and correlated this activity with the content of Fluoride, Aluminium and Strontium ions in their eluates. Methods: Elution samples of biomaterials were prepared in sterile tissue culture medium and the medium was tested for toxicity by an assay of cell survival/proliferation (MTT test) and apoptosis (Annexin V FITC Detection Kit). Concentrations of Fluoride, Aluminium and Strontium ions were tested by appropriate methods in the same eluates. Results: Cell survival ranged between 79.62% (Fuji Triage) to 1.5% (Fuji Plus) and most dead DPSCs were in the stage of late apoptosis. Fluoride release correlated with cytotoxicity of GICs, while Aluminium and Strontium ions, present in significant amount in eluates of tested GICs did not. Significance: Fuji Plus, Vitrebond and Fuji VIII, which released fluoride in higher quantities than other GICs, were highly toxic to human DPSCs. Opposite, low levels of released fluoride correlated to low cytotoxic effect of Composit, Fuji I and Fuji Triage.
PB  - Bentham Science Publ Ltd, Sharjah
T2  - Medicinal Chemistry
T1  - Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release
VL  - 8
IS  - 1
SP  - 40
EP  - 45
DO  - 10.2174/157340612799278351
ER  - 

@article{
author = "Kanjevac, Tatjana and Milovanović, Marija and Volarević, Vladislav and Lukić, Miodrag L. and Arsenijević, Nebojša and Marković, Dejan and Zdravković, Nebojša and Tešić, Živoslav and Lukić, Aleksandra",
year = "2012",
abstract = "Objectives: Glass ionomer cements (GICs) are commonly used as restorative materials. Responses to GICs differ among cell types and it is therefore of importance to thoroughly investigate the influence of these restorative materials on pulp stem cells that are potential source for dental tissue regeneration. Eight biomaterials were tested: Fuji I, Fuji II, Fuji VIII, Fuji IX, Fuji Plus, Fuji Triage, Vitrebond and Composit. We compared their cytotoxic activity on human dental pulp stem cells (DPSC) and correlated this activity with the content of Fluoride, Aluminium and Strontium ions in their eluates. Methods: Elution samples of biomaterials were prepared in sterile tissue culture medium and the medium was tested for toxicity by an assay of cell survival/proliferation (MTT test) and apoptosis (Annexin V FITC Detection Kit). Concentrations of Fluoride, Aluminium and Strontium ions were tested by appropriate methods in the same eluates. Results: Cell survival ranged between 79.62% (Fuji Triage) to 1.5% (Fuji Plus) and most dead DPSCs were in the stage of late apoptosis. Fluoride release correlated with cytotoxicity of GICs, while Aluminium and Strontium ions, present in significant amount in eluates of tested GICs did not. Significance: Fuji Plus, Vitrebond and Fuji VIII, which released fluoride in higher quantities than other GICs, were highly toxic to human DPSCs. Opposite, low levels of released fluoride correlated to low cytotoxic effect of Composit, Fuji I and Fuji Triage.",
publisher = "Bentham Science Publ Ltd, Sharjah",
journal = "Medicinal Chemistry",
title = "Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release",
volume = "8",
number = "1",
pages = "40-45",
doi = "10.2174/157340612799278351"
}

Kanjevac, T., Milovanović, M., Volarević, V., Lukić, M. L., Arsenijević, N., Marković, D., Zdravković, N., Tešić, Ž.,& Lukić, A.. (2012). Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release. in Medicinal Chemistry
Bentham Science Publ Ltd, Sharjah., 8(1), 40-45.
https://doi.org/10.2174/157340612799278351

Kanjevac T, Milovanović M, Volarević V, Lukić ML, Arsenijević N, Marković D, Zdravković N, Tešić Ž, Lukić A. Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release. in Medicinal Chemistry. 2012;8(1):40-45.
doi:10.2174/157340612799278351 .

Kanjevac, Tatjana, Milovanović, Marija, Volarević, Vladislav, Lukić, Miodrag L., Arsenijević, Nebojša, Marković, Dejan, Zdravković, Nebojša, Tešić, Živoslav, Lukić, Aleksandra, "Cytotoxic Effects of Glass Ionomer Cements on Human Dental Pulp Stem Cells Correlate with Fluoride Release" in Medicinal Chemistry, 8, no. 1 (2012):40-45,
https://doi.org/10.2174/157340612799278351 . .