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Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis

Authorized Users Only
2006
Authors
Stefanović, Gordana
Marković, Dragana
Ilić, Vesna
Brajović, Gavrilo
Petrović, Sonja
Milošević-Jovčić, Nadežda
Article (Published version)
Metadata
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Abstract
Background: Altered glycosylation of immunoglobulin G (IgG) has been found to affect certain immunological activities of IgG and to correlate with increased inflammation in various disease states. This work deals with the changes in distribution and galactosylation of IgG subclasses present in saliva and gingival crevicular fluid (GCF) of patients with initial and advanced periodontitis and of normal controls. Methods: IgG subclasses were quantified by dot-blot assay, and the degrees of expression of galactose in the total IgG and its individual subclasses were estimated by lectin immunoblot assay after sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) separation of IgG and by capture enzyme-linked immunosorbent assay (ELISA), using biotinylated Ricinus communis (RCA-1) and Bandeiraea simplicifolia (BS-II) lectins. Results: The distribution of IgG subclasses in both fluids was found to differ in periodontal patients compared to normal controls. In the periodontitis... saliva and GCF, the IgG2 subclass dominated quantitatively, regardless of periodontal status. However, galactose was found to be expressed in IgG heavy chains in normal controls and patients with initial periodontitis but not, or at barely detectable levels, in advanced periodontitis. Conclusion: The results suggest that the shift toward hypo-galactosylated glycoforms may occur during the process of inflammation of the gingiva.

Keywords:
immunoglobulin G / periodontitis
Source:
Journal of Periodontology, 2006, 77, 11, 1887-1893
Publisher:
  • Amer Acad Periodontology, Chicago

DOI: 10.1902/jop.2006.060049

ISSN: 0022-3492

PubMed: 17076615

WoS: 000243801100013

Scopus: 2-s2.0-33751278875
[ Google Scholar ]
12
13
URI
https://smile.stomf.bg.ac.rs/handle/123456789/1318
Collections
  • Radovi istraživača
Institution/Community
Stomatološki fakultet
TY  - JOUR
AU  - Stefanović, Gordana
AU  - Marković, Dragana
AU  - Ilić, Vesna
AU  - Brajović, Gavrilo
AU  - Petrović, Sonja
AU  - Milošević-Jovčić, Nadežda
PY  - 2006
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1318
AB  - Background: Altered glycosylation of immunoglobulin G (IgG) has been found to affect certain immunological activities of IgG and to correlate with increased inflammation in various disease states. This work deals with the changes in distribution and galactosylation of IgG subclasses present in saliva and gingival crevicular fluid (GCF) of patients with initial and advanced periodontitis and of normal controls. Methods: IgG subclasses were quantified by dot-blot assay, and the degrees of expression of galactose in the total IgG and its individual subclasses were estimated by lectin immunoblot assay after sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) separation of IgG and by capture enzyme-linked immunosorbent assay (ELISA), using biotinylated Ricinus communis (RCA-1) and Bandeiraea simplicifolia (BS-II) lectins. Results: The distribution of IgG subclasses in both fluids was found to differ in periodontal patients compared to normal controls. In the periodontitis saliva and GCF, the IgG2 subclass dominated quantitatively, regardless of periodontal status. However, galactose was found to be expressed in IgG heavy chains in normal controls and patients with initial periodontitis but not, or at barely detectable levels, in advanced periodontitis. Conclusion: The results suggest that the shift toward hypo-galactosylated glycoforms may occur during the process of inflammation of the gingiva.
PB  - Amer Acad Periodontology, Chicago
T2  - Journal of Periodontology
T1  - Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis
VL  - 77
IS  - 11
SP  - 1887
EP  - 1893
DO  - 10.1902/jop.2006.060049
ER  - 
@article{
author = "Stefanović, Gordana and Marković, Dragana and Ilić, Vesna and Brajović, Gavrilo and Petrović, Sonja and Milošević-Jovčić, Nadežda",
year = "2006",
abstract = "Background: Altered glycosylation of immunoglobulin G (IgG) has been found to affect certain immunological activities of IgG and to correlate with increased inflammation in various disease states. This work deals with the changes in distribution and galactosylation of IgG subclasses present in saliva and gingival crevicular fluid (GCF) of patients with initial and advanced periodontitis and of normal controls. Methods: IgG subclasses were quantified by dot-blot assay, and the degrees of expression of galactose in the total IgG and its individual subclasses were estimated by lectin immunoblot assay after sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) separation of IgG and by capture enzyme-linked immunosorbent assay (ELISA), using biotinylated Ricinus communis (RCA-1) and Bandeiraea simplicifolia (BS-II) lectins. Results: The distribution of IgG subclasses in both fluids was found to differ in periodontal patients compared to normal controls. In the periodontitis saliva and GCF, the IgG2 subclass dominated quantitatively, regardless of periodontal status. However, galactose was found to be expressed in IgG heavy chains in normal controls and patients with initial periodontitis but not, or at barely detectable levels, in advanced periodontitis. Conclusion: The results suggest that the shift toward hypo-galactosylated glycoforms may occur during the process of inflammation of the gingiva.",
publisher = "Amer Acad Periodontology, Chicago",
journal = "Journal of Periodontology",
title = "Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis",
volume = "77",
number = "11",
pages = "1887-1893",
doi = "10.1902/jop.2006.060049"
}
Stefanović, G., Marković, D., Ilić, V., Brajović, G., Petrović, S.,& Milošević-Jovčić, N.. (2006). Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis. in Journal of Periodontology
Amer Acad Periodontology, Chicago., 77(11), 1887-1893.
https://doi.org/10.1902/jop.2006.060049
Stefanović G, Marković D, Ilić V, Brajović G, Petrović S, Milošević-Jovčić N. Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis. in Journal of Periodontology. 2006;77(11):1887-1893.
doi:10.1902/jop.2006.060049 .
Stefanović, Gordana, Marković, Dragana, Ilić, Vesna, Brajović, Gavrilo, Petrović, Sonja, Milošević-Jovčić, Nadežda, "Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis" in Journal of Periodontology, 77, no. 11 (2006):1887-1893,
https://doi.org/10.1902/jop.2006.060049 . .

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