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dc.creatorStefanović, Gordana
dc.creatorMarković, Dragana
dc.creatorIlić, Vesna
dc.creatorBrajović, Gavrilo
dc.creatorPetrović, Sonja
dc.creatorMilošević-Jovčić, Nadežda
dc.date.accessioned2020-07-02T12:12:52Z
dc.date.available2020-07-02T12:12:52Z
dc.date.issued2006
dc.identifier.issn0022-3492
dc.identifier.urihttp://smile.stomf.bg.ac.rs/handle/123456789/1318
dc.description.abstractBackground: Altered glycosylation of immunoglobulin G (IgG) has been found to affect certain immunological activities of IgG and to correlate with increased inflammation in various disease states. This work deals with the changes in distribution and galactosylation of IgG subclasses present in saliva and gingival crevicular fluid (GCF) of patients with initial and advanced periodontitis and of normal controls. Methods: IgG subclasses were quantified by dot-blot assay, and the degrees of expression of galactose in the total IgG and its individual subclasses were estimated by lectin immunoblot assay after sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) separation of IgG and by capture enzyme-linked immunosorbent assay (ELISA), using biotinylated Ricinus communis (RCA-1) and Bandeiraea simplicifolia (BS-II) lectins. Results: The distribution of IgG subclasses in both fluids was found to differ in periodontal patients compared to normal controls. In the periodontitis saliva and GCF, the IgG2 subclass dominated quantitatively, regardless of periodontal status. However, galactose was found to be expressed in IgG heavy chains in normal controls and patients with initial periodontitis but not, or at barely detectable levels, in advanced periodontitis. Conclusion: The results suggest that the shift toward hypo-galactosylated glycoforms may occur during the process of inflammation of the gingiva.en
dc.publisherAmer Acad Periodontology, Chicago
dc.rightsrestrictedAccess
dc.sourceJournal of Periodontology
dc.subjectimmunoglobulin Gen
dc.subjectperiodontitisen
dc.titleHypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitisen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМилошевић-Јовчић, Надежда; Петровић, Соња; Илић, Весна; Брајовић, Гаврило; Стефановић, Гордана; Марковић, Драгана;
dc.citation.volume77
dc.citation.issue11
dc.citation.spage1887
dc.citation.epage1893
dc.citation.other77(11): 1887-1893
dc.citation.rankM22
dc.identifier.wos000243801100013
dc.identifier.doi10.1902/jop.2006.060049
dc.identifier.pmid17076615
dc.identifier.scopus2-s2.0-33751278875
dc.identifier.rcubconv_2727
dc.type.versionpublishedVersion


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