Show simple item record

dc.creatorStrahinić, I.
dc.creatorBusarcević, M.
dc.creatorPavlica, Dušan
dc.creatorMilašin, Jelena
dc.creatorGolić, N.
dc.creatorTopisirović, L.
dc.date.accessioned2020-07-02T12:14:10Z
dc.date.available2020-07-02T12:14:10Z
dc.date.issued2007
dc.identifier.issn0902-0055
dc.identifier.urihttp://smile.stomf.bg.ac.rs/handle/123456789/1338
dc.description.abstractIntroduction: The objective of this study was to characterize the lactobacilli from the human oral cavity as a potential source of probiotic strains. Methods: Samples were collected from four different locations within the oral cavity: surface of healthy tooth, oral mucous membrane, surface of tooth decay and deep tooth decay. On the basis of morphological and biochemical properties eight categories were formed and 26 isolates were selected for further characterization. The isolates were determined as Lactobacillus sp. using primers specific for 16S rDNA. Sequencing of 16S rDNA genes and repetitive sequence-based polymerase chain reactions were used for determination to species and subspecies levels. Results: Predominant species were Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus salivarius and Lactobacillus paracasei subsp. paracasei, while Lactobacillus acidophilus, Lactobacillus cellobiosus, Lactobacillus delbrueckii subsp. lactis and Lactobacillus gasseri were also present. The isolates Lactobacillus salivarius BGHO1, Lactobacillus fermentum BGHO36 and BGHO64, Lactobacillus gasseri BGHO89 and Lactobacillus delbrueckii subsp. lactis BGHO99 exhibited antagonistic action on the growth of Staphylococcus aureus, Enterococcus faecalis, Micrococcus flavus, Salmonella enteritidis, Streptococcus pneumoniae and Streptococcus mutans, but not on growth of Candida albicans. Moreover, the isolates L. salivarius BGHO1 and L. gasseri BGHO89 were tolerant to low pH and high concentration of bile salts. Conclusion: Taken together, these findings imply that L. salivarius BGHO1 and L. gasseri BGHO89 might be subjects for additional investigation as potential probiotic strains.en
dc.publisherWiley, Hoboken
dc.rightsrestrictedAccess
dc.sourceOral Microbiology & Immunology
dc.subject16S rDNAen
dc.subjectlactobacillien
dc.subjectoral microfloraen
dc.subjectprobioticen
dc.subjectrepetitive sequence-based polymerase chain reactionen
dc.titleMolecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidatesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractГолић, Н.; Страхинић, И.; Павлица, Душан; Тописировић, Л.; Милашин, Јелена; Бусарцевић, М.;
dc.citation.volume22
dc.citation.issue2
dc.citation.spage111
dc.citation.epage117
dc.citation.other22(2): 111-117
dc.citation.rankM22
dc.identifier.wos000244279800006
dc.identifier.doi10.1111/j.1399-302X.2007.00331.x
dc.identifier.pmid17311634
dc.identifier.scopus2-s2.0-33847043017
dc.identifier.rcubconv_2728
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record