The effect of caffeic acid phenethyl ester on healing capacity and repair of the dentin-pulp complex: In vivo study

Abstract

The aim of the present study was to investigate the effects of caffeic acid phenethyl ester (CAPE) on wound healing capacity and repair of the pulp dentin complex on an experimental model. Eight young male pigs were used in this study. Arteficially preformed pulp lesions were treated either with calcium hydroxide (control group) or with CAPE powder (experimental group). The material for histological analysis was 4 and 14 weeks after pulp capping. Results of the histological analysis revealed that inflammatory reactions occurred in both groups. In the control group inflammation could be scored as mild to moderate, with a tendency to decrease during the observed period, while in the experimental group it was scored as severe in most of the specimens. Dentin bridge formation and complete reparation of the dentin-pulp complex was observed in most of the specimens of the control group, while the repair process in the experimental group was characterized by fibrous tissue proliferation. In the experimental group severe haemorrhagia, tissue oedema and dentin resorption were also observed. Based on reported results it could be concluded that capping material containing CAPE could not enhance the repair of the dentin-pulp complex, and healing of the dentin-pulp complex was characterized by scar tissue formation, rather than dentin bridge formation.

Cilj rad je bio da se ispita efekat fenetil estra kafeinske kiseline na proces zarastanja rane i reparaciju dentinsko-pulpnog kompleksa. Istraživanje je izvršeno na 8 svinja muškog pola t.m. 60-65 kg. Njima je arteficijelno napravljena lezija u dentinsko-pulpnom kompleksu, koja je tretirana kalcijum hidroksidom (kontrolna grupa) ili fenetil estrom kafeinske kiseline (eksperimentalna grupa). Materijal za histološku analizu uzet je 4 i 14 nedelja nakon prekrivanja pulpe. Rezultati histološ ke analize su ukazali da se u obe grupe uzoraka pojavila zapaljenska reakcija. U kontrolnoj grupi, ona je ocenjena kao blaga do umerena, sa tendencijom smanjivanja tokom observacionog perioda, dok je u eksperimentalnoj grupi, u najvećem broju uzoraka ona ocenjena kao teška. Kompletna reparacija dentinsko- pulpnog kompleksa sa stvaranjem dentinskog mostića uočena je samo u kontrolnoj grupi, dok je u eksperimentalnoj, zarastanje bilo karakterisano vezivno tkivnom proliferacijom. U velikom broju uzoraka eksperimentalne grupe uočeni su znaci krvarenja, edema i resorpcije dentina. Na osnovu rezultata dobijenih u ovoj studiji može se reći da fenetil estar kafeinske kiseline ne podstiče reparativne procese u dentinsko-pulpnom kompleksu, tako da proces zarastanja rane nije bio praćen formiranjem reparativnog dentina već vezivno-tkivnog ožiljka.