Comparison of the hybrid layer formed by Silorane adhesive, one-step self-etch and etch and rinse systems using confocal micro-Raman spectroscopy and SEM
Abstract
Objectives: To determine the extent of the hybrid layer (HL) of the novel adhesive system (Silorane, 3M ESPE) compared to one-step and etch and rinse adhesive systems, using 2D confocal micro-Roman spectroscopy and SEM. Methods: Silorane adhesive system was compared to two one-step self-etch (G Bond, GC; AdheSE One, Ivoclar Vivadent) and etch and rinse (Excite, Ivoclar Vivadent) as controls. Adhesives were applied to human dentine, cured and sections prepared perpendicular to the flat adhesive-dentine surface. Two-dimensional micro-Roman mapping was performed over 20 mu m x 20 mu m areas across the adhesive-dentine interface. SEM micrographs of the same specimens were obtained. One-way ANOVA (Tukey's post-test) was used to analyse the differences in HL thickness among the four adhesive systems and the paired t-test to compare the results obtained by micro-Roman and SEM for each adhesive. Results: Silorane adhesive system formed a HL of comparable thickness to methacrylate-based but thi...cker than the acrylamide-based one-step self-etch adhesives from SEM analysis. A gradual decrease in adhesive penetration was observed in all systems. A 1-mu m zone, between the cured primer and bond was identified by Raman but not visualised on SEM. Compared to SEM, HL calculated from Raman data were always greater. Conclusions: Micro-Roman spectroscopy gives a more precise indication of dentine demineralisation and monomer infiltration and highlights the intermediate zone of similar to 1 mu m between Silorane primer and bond, not visible with SEM at x10,000 magnification. Giving as it does, a compilation of many linear scans it allows HL mean values to be calculated.
Keywords:
confocal micro-Raman / spectroscopy / SEM / hybrid layer / self-etch adhesivesSource:
Journal of Dentistry, 2008, 36, 9, 683-691Publisher:
- Elsevier Sci Ltd, Oxford
DOI: 10.1016/j.jdent.2008.04.016
ISSN: 0300-5712
PubMed: 18550251
WoS: 000259659200003
Scopus: 2-s2.0-48549087606
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Institution/Community
Stomatološki fakultetTY - JOUR AU - Santini, Ario AU - Miletić, Vesna PY - 2008 UR - https://smile.stomf.bg.ac.rs/handle/123456789/1456 AB - Objectives: To determine the extent of the hybrid layer (HL) of the novel adhesive system (Silorane, 3M ESPE) compared to one-step and etch and rinse adhesive systems, using 2D confocal micro-Roman spectroscopy and SEM. Methods: Silorane adhesive system was compared to two one-step self-etch (G Bond, GC; AdheSE One, Ivoclar Vivadent) and etch and rinse (Excite, Ivoclar Vivadent) as controls. Adhesives were applied to human dentine, cured and sections prepared perpendicular to the flat adhesive-dentine surface. Two-dimensional micro-Roman mapping was performed over 20 mu m x 20 mu m areas across the adhesive-dentine interface. SEM micrographs of the same specimens were obtained. One-way ANOVA (Tukey's post-test) was used to analyse the differences in HL thickness among the four adhesive systems and the paired t-test to compare the results obtained by micro-Roman and SEM for each adhesive. Results: Silorane adhesive system formed a HL of comparable thickness to methacrylate-based but thicker than the acrylamide-based one-step self-etch adhesives from SEM analysis. A gradual decrease in adhesive penetration was observed in all systems. A 1-mu m zone, between the cured primer and bond was identified by Raman but not visualised on SEM. Compared to SEM, HL calculated from Raman data were always greater. Conclusions: Micro-Roman spectroscopy gives a more precise indication of dentine demineralisation and monomer infiltration and highlights the intermediate zone of similar to 1 mu m between Silorane primer and bond, not visible with SEM at x10,000 magnification. Giving as it does, a compilation of many linear scans it allows HL mean values to be calculated. PB - Elsevier Sci Ltd, Oxford T2 - Journal of Dentistry T1 - Comparison of the hybrid layer formed by Silorane adhesive, one-step self-etch and etch and rinse systems using confocal micro-Raman spectroscopy and SEM VL - 36 IS - 9 SP - 683 EP - 691 DO - 10.1016/j.jdent.2008.04.016 ER -
@article{ author = "Santini, Ario and Miletić, Vesna", year = "2008", abstract = "Objectives: To determine the extent of the hybrid layer (HL) of the novel adhesive system (Silorane, 3M ESPE) compared to one-step and etch and rinse adhesive systems, using 2D confocal micro-Roman spectroscopy and SEM. Methods: Silorane adhesive system was compared to two one-step self-etch (G Bond, GC; AdheSE One, Ivoclar Vivadent) and etch and rinse (Excite, Ivoclar Vivadent) as controls. Adhesives were applied to human dentine, cured and sections prepared perpendicular to the flat adhesive-dentine surface. Two-dimensional micro-Roman mapping was performed over 20 mu m x 20 mu m areas across the adhesive-dentine interface. SEM micrographs of the same specimens were obtained. One-way ANOVA (Tukey's post-test) was used to analyse the differences in HL thickness among the four adhesive systems and the paired t-test to compare the results obtained by micro-Roman and SEM for each adhesive. Results: Silorane adhesive system formed a HL of comparable thickness to methacrylate-based but thicker than the acrylamide-based one-step self-etch adhesives from SEM analysis. A gradual decrease in adhesive penetration was observed in all systems. A 1-mu m zone, between the cured primer and bond was identified by Raman but not visualised on SEM. Compared to SEM, HL calculated from Raman data were always greater. Conclusions: Micro-Roman spectroscopy gives a more precise indication of dentine demineralisation and monomer infiltration and highlights the intermediate zone of similar to 1 mu m between Silorane primer and bond, not visible with SEM at x10,000 magnification. Giving as it does, a compilation of many linear scans it allows HL mean values to be calculated.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Journal of Dentistry", title = "Comparison of the hybrid layer formed by Silorane adhesive, one-step self-etch and etch and rinse systems using confocal micro-Raman spectroscopy and SEM", volume = "36", number = "9", pages = "683-691", doi = "10.1016/j.jdent.2008.04.016" }
Santini, A.,& Miletić, V.. (2008). Comparison of the hybrid layer formed by Silorane adhesive, one-step self-etch and etch and rinse systems using confocal micro-Raman spectroscopy and SEM. in Journal of Dentistry Elsevier Sci Ltd, Oxford., 36(9), 683-691. https://doi.org/10.1016/j.jdent.2008.04.016
Santini A, Miletić V. Comparison of the hybrid layer formed by Silorane adhesive, one-step self-etch and etch and rinse systems using confocal micro-Raman spectroscopy and SEM. in Journal of Dentistry. 2008;36(9):683-691. doi:10.1016/j.jdent.2008.04.016 .
Santini, Ario, Miletić, Vesna, "Comparison of the hybrid layer formed by Silorane adhesive, one-step self-etch and etch and rinse systems using confocal micro-Raman spectroscopy and SEM" in Journal of Dentistry, 36, no. 9 (2008):683-691, https://doi.org/10.1016/j.jdent.2008.04.016 . .