Influence of magnetic field to microbes in oral environment

Abstract

From the very beginning of life, living organisms were exposed to the influence of geoelectric and geomagnetic fields, with established balance with this respect. Development of civilization has been distorted balance and harmony, imposing the extreme need for magnetism. Respecting positive effects of magnetic field to tissues, especially 'bactericide' effect, this investigation was conducted on the aim to assess the influence of magnetic field to oral microbes. Materials and method. This investigation was carried to in vitro. To obtain adequate samples of deposits or specimens of dental plaque, were from certain microbes which were isolated from human mouth. Microbes isolated from samples were Streptococcus parasanguis, Staphylococcus epidermidis, Rodococcus equi and Candida albicans. Turbidimetry array was applied for investigation of this kind. The method of spectrophotometry was applied, to obtain optical den­sity (OD) value and to establish indirectly a growth of intensity of sowed microbe's stains on the basis of modified OD of agar. Investigation was carried on two level of density of microbes of 1 CFU/ml as well as of 10 CFU/ml after 24h period and 48h period of incubation of microbes. Results. Positive effect of magnetic field upon dental plaque of bacteria reduction in vitro was recognized. At the baseline-initial 24 hours period of measurement of exposition to magnetic field, the count of all isolated mi­crobes has been significantly reduced, in the both of agar of increased density as well as of agar with reduced OD. Over the time, the decrement of influence of magnets versus microbes in vitro has been detected. Conclusion. Count of this influence of the magnet to microbes is of extreme importance in parodontology. This way, the positive influence of magnet to supporting tissues and quality and durability of overdenture was confirmed. .

Sva živa bića su izložena dejstvu geoelektričnog i geomagnetskog polja i u tom smislu je ostvarena ravnoteža. Sa razvojem civilizacije ta ravnoteža se narušava u smislu da slabi uticaj geomagnetnog polja i postoji t.z.v. 'glad' za magnetizmom. Imajući u vidu pozitivan uticaj magneta na sva biološka tkiva, posebno 'baktericidni' efekat israživanje je sprovedeno sa ciljem da se ispita uticaj magnetnog polja na oralnu mikrofloru. Materijal i metod: Istraživanja su sprovedena in vitro. Uzorci, tj. brisevi zubnih naslaga poticali su sa zuba pacijenata Stomatološkog fakulteta u Beogradu. Iz uzoraka su izolovani: Streptococcus parasanguis, Staphylococcus epidermidis, Rodococcus equi i Candida albicans. Korišćena je metoda turbidimetrija gde se spektrofotometrom na osnovu optičke gustine bujona indirektno utvrđuje intenzitet rasta zasejanih mikro-organizama. Ispitivanje je vršeno sa 2 gustine mikroorganizama 1 i 10 CFU/ml i to nakon 24 i 48 h inkubacije mikroorganizama. Rezultati pokazuju da je došlo do redukcije broja bakterija u in vitro uslovima. U prvih 24 h izloženosti uticaju magnetnog polja ta redukcija je bila značajna kako u bujonima veće tako i manje gustine. Tokom vremena uticaj magneta na brojnost mikroflore u in vitro uslovima opada. Zaključak Ovakav uticaj magneta na brojnost mikroorganizama je značajan u parodontologiji pa samim tim ima pozitivan uticaj na potporna tkiva ispod supraden-talnih proteza, na njihov kvalitet i trajnost. .